CN108522145A - A kind of white fungus culture medium and cultivating white fungus method - Google Patents
A kind of white fungus culture medium and cultivating white fungus method Download PDFInfo
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- CN108522145A CN108522145A CN201810446884.1A CN201810446884A CN108522145A CN 108522145 A CN108522145 A CN 108522145A CN 201810446884 A CN201810446884 A CN 201810446884A CN 108522145 A CN108522145 A CN 108522145A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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Abstract
The invention belongs to field of edible fungus culture, and in particular to a kind of white fungus culture medium and cultivating white fungus method.White fungus culture medium includes that the raw material of following parts by weight is made：75~85 parts of cotton seed hulls, 15~25 parts of wheat bran and contain Cr3+Water soluble compound contains Cr3+Cr in white fungus culture medium after water soluble compound addition3+Content be 12~18mg/kg, above-mentioned culture medium access white fungus strain is subjected to Mycelium culture, Mycelium culture carries out under dark ventilation condition, daily ventilation 2~3 times, 15~30min every time, Mycelium culture include germination period, growth stage and maturity period, the output increased of white fungus 15%~22%, the white fungus ear meat of production is plump, and the yield of cultural method white fungus through the invention has obtained significant raising and do not influenced on the quality of white fungus.
Description
Technical field
The invention belongs to field of edible fungus culture, and in particular to a kind of white fungus culture medium and cultivating white fungus method.
Background technology
White fungus (Tremella fuciformis) is also known as tremella, belongs to Eumycota, Basidiomycetes, Tremellales, Tremella,
It is sweet in flavor, light, mild-natured, nontoxic, there is the effect of tonifying spleen appetizing, QI invigorating gut purge, nourishing Yin and moistening lung, obtains the good reputation of " hat in bacterium ", be me
A kind of extremely precious edible medicine dual-purpose mushroom of state.White fungus rich in nutrition content, containing protein, minerals, dietary fiber etc.,
Wherein protein is at least made of 17 kinds of amino acid.
Wild white fungus rare numbers, the in ancient times expensive tonic of generic name.White fungus is segment wood cultivated to consume huge, mesh to timber resources
Before, those skilled in the art may produce white fungus yield and its nutrition, quality cultivating white fungus culture medium prescription, cultivation condition etc.
The raw factor influenced is studied, under the premise of ensureing white fungus nutrition, quality, shorten the white fungus production cycle, improve
White fungus yield.With the continuous increase of white fungus market demand, the problem of white fungus yield is urgent need to resolve how is improved.
Invention content
The technical problem to be solved by the present invention is to：It provides and a kind of ensureing white fungus quality improve white fungus yield while
White fungus culture medium and cultivating white fungus method.
In order to solve the above-mentioned technical problem, the technical solution adopted by the present invention is：A kind of white fungus culture medium, including weigh as follows
The raw material of amount part is made：75~85 parts of cotton seed hulls, 15~25 parts of wheat bran and contain Cr3+Water soluble compound；
It is described to contain Cr3+Cr in white fungus culture medium after water soluble compound addition3+Content be 12~18mg/kg.
The present invention also provides a kind of cultivating white fungus methods, include the following steps：
Step 1, by siccative and water according to 50~55：45~50 weight ratio is hybridly prepared into white fungus culture medium, described dry
Material mixes after being crushed by the raw material of following parts by weight：75~85 parts of cotton seed hulls, 15~25 parts of wheat bran and contain Cr3+Water solubilityization
Object is closed, Cr in the white fungus culture medium3+Content be 12~18mg/kg；
Step 2, by the culture medium obtained by step 1 after mixing, bacteria stick is made after compacting pack, is accessed after bacteria stick sterilizing
White fungus strain carries out Mycelium culture, and Mycelium culture carries out under dark ventilation condition, daily ventilation 2~3 times, every time
15~30min, Mycelium culture include following growth phase：
A, germination period：Inoculation was germination period in 15 days, and first three days temperature is maintained at 26~27 DEG C after inoculation, and air is relatively wet
Degree control is 60%~65%, and a day temperature is maintained at 25~26 DEG C from the 4th day to the 15th, relative air humidity control 60%~
65%；
B, the growth stage：16th day to 35 days, temperature was maintained at 23 DEG C~25 DEG C, relative air humidity control 55%~
65%；
C, the maturity period：After 35 days, mycelia enters stage of ripeness temperature and is maintained at 18~20 DEG C, and relative air humidity control exists
65%~70%, stop water spray within 1~2 day before harvesting white fungus.
The beneficial effects of the present invention are：Through repetition test, the present invention adds in compost contains Cr3+Water-soluble chemical combination
Object controls its a concentration of 12~18mg/kg, culture medium is made with cotton seed hulls and wheat bran, the culture prepared using inventive formulation
Base cultivates white fungus, and the white fungus ear meat of the output increased of white fungus 15%~22%, production is plump, cultural method through the invention
The yield of white fungus has obtained significant raising and has not been influenced on the quality of white fungus.
Specific implementation mode
To explain the technical content, the achieved purpose and the effect of the present invention in detail, it is explained below in conjunction with embodiment.
The design of most critical of the present invention is：Addition contains Cr in culture medium dispensing3+It is a concentration of to control its for water soluble compound
12~18mg/kg cultivates white fungus, the output increased of white fungus 15%~22%, product using culture medium prepared by inventive formulation
Matter does not have difference with conventional cultivation white fungus.
The present invention provides a kind of white fungus culture medium, including the raw material of following parts by weight is made：75~85 parts of cotton seed hulls, wheat bran
15~25 parts and contain Cr3+Water soluble compound；
It is described to contain Cr3+Cr in white fungus culture medium after water soluble compound addition3+Content be 12~18mg/kg.
Further, including the raw material of following parts by weight is made：80 parts of cotton seed hulls, 20 parts of wheat bran and chromium chloride solution,
Cr in white fungus culture medium after the chromium chloride solution addition3+Content be 14~16mg/kg.
As can be seen from the above description, the beneficial effects of the present invention are：CrCl is introduced in culture medium dispensing3.By repeatedly
Experiment, it is found that the white fungus yield cultivated is improved significantly, and quality does not have notable difference with conventional cultivation white fungus.
The present invention also provides a kind of cultivating white fungus methods, include the following steps：
Step 1, by siccative and water according to 50~55：45~50 weight ratio is hybridly prepared into white fungus culture medium, described dry
Material mixes after being crushed by the raw material of following parts by weight：75~85 parts of cotton seed hulls, 15~25 parts of wheat bran and contain Cr3+Water solubilityization
Object is closed, Cr in the white fungus culture medium3+Content be 12~18mg/kg；
Step 2, by the culture medium obtained by step 1 after mixing, bacteria stick is made after compacting pack, is accessed after bacteria stick sterilizing
White fungus strain carries out Mycelium culture, and Mycelium culture carries out under dark ventilation condition, daily ventilation 2~3 times, every time
15~30min, Mycelium culture include following growth phase：
A, germination period：Inoculation was germination period in 15 days, and first three days temperature is maintained at 26~27 DEG C after inoculation, and air is relatively wet
Degree control is 60%~65%, and a day temperature is maintained at 25~26 DEG C from the 4th day to the 15th, relative air humidity control 60%~
65%；
B, the growth stage：16th day to 35 days, temperature was maintained at 23 DEG C~25 DEG C, relative air humidity control 55%~
65%；
C, the maturity period：After 35 days, mycelia enters stage of ripeness temperature and is maintained at 18~20 DEG C, and relative air humidity control exists
65%~70%, stop water spray within 1~2 day before harvesting white fungus.
Cr3+Physiological action：Cr3+As micro elements needed by human, important physiological action is played in vivo.In U.S.
State, chromium picolinate (CrPic) are a kind of widely used dietary supplements.U.S. FDA suggests Cr3+Average daily intake, man
Property is 35ug, women 25ug.GB/T 5009.195-2003《The measurement of pyridine carboxylic acid chromium content in health food》In point out,
CrPic can pass through cell membrane and directly act on histocyte, have important booster action to the treatment of diabetes.2012
《Food enrichment uses standard》Regulation, is allowed for nutrition fortifier and the compound source of special diet food, chromium
Compound be chromium sulfate (Cr2(SO4)3) and chromium chloride (CrCl3).But as food refines and processes, lead to Cr in food3+
Be largely lost, may influence the metabolism of the large biological molecules such as nucleic acid, protein, carbohydrate and fat, it is appropriate in diet
Ground supplements Cr3+Become more important, Cr is supplemented in diet3+The loss of body fat is increased, while keeping muscle；Overcome pancreas
Insulin resistance and improvement diabetes；The formation of free radical is inhibited to reduce the physiological reactions such as total cholesterol and low-density lipoprotein, it is right
Body has positive meaning.
As can be seen from the above description, the beneficial effects of the present invention are：Through repetition test, the present invention adds in compost to be contained
Cr3+Water soluble compound is controlled its a concentration of 12~18mg/kg, culture medium is made with cotton seed hulls and wheat bran, is matched using the present invention
The culture medium cultivation white fungus just prepared, the white fungus ear meat of the output increased of white fungus 15%~22%, production is plump, by this hair
The yield of bright cultural method white fungus has obtained significant raising and has not been influenced on the quality of white fungus.
Further, the step 1 is specially：By siccative and water according to 1：1 weight ratio is hybridly prepared into white fungus culture
Base, the siccative mix after being crushed by the raw material of following parts by weight：80 parts of cotton seed hulls, 20 parts of wheat bran and chromium chloride are water-soluble
Liquid, Cr in the white fungus culture medium3+Content be 14~16mg/kg.
Seen from the above description, when chromium chloride additive amount is in optimum range, can significantly improve white fungus yield 15~
22%, and white fungus quality is influenced without conspicuousness.
Further, miscellaneous bacteria should be checked for during the Mycelium culture, the bacterium bag with miscellaneous bacteria is removed in time.
Seen from the above description, the bacterium bag with miscellaneous bacteria is removed in time, prevents other bacterium bags of living contaminants.
Further, the criterion to come to the ripening period in the step 2 is that fructification auricle all stretches, and growth stops
Only, there is no microtia stamen；Color is fresh white or cream-coloured.
Further, the weight of each bacteria stick is 1.5kg in the step 2.
Embodiment 1
A kind of white fungus culture medium, including the raw material of following parts by weight are made：Cotton seed hulls 0.63kg；Wheat bran 0.12kg, addition
Chromium chloride solution, Cr in middle white fungus culture medium after chromium chloride solution addition3+Content be 12mg/kg.
The present invention also provides a kind of cultivating white fungus methods, include the following steps：Step 1, by siccative and water according to 50~55：
45~50 weight ratio is hybridly prepared into culture medium, and after mixing, hand of being subject to is micro- to hold agglomerating but not flowing water, and moisture after bacterium bag is made
It is 60%~65%, the siccative mixes after being crushed by the raw material of following weight：Cotton seed hulls 0.63kg；Wheat bran 0.12kg,
Chromium chloride solution is added, Cr in white fungus culture medium after chromium chloride solution addition3+Content be 12mg/kg；
Step 2, by the culture medium obtained by step 1 after mixing, bacteria stick is made after compacting pack, is accessed after bacteria stick sterilizing
White fungus strain carries out Mycelium culture, and Mycelium culture carries out under dark ventilation condition, daily ventilation 2~3 times, every time
15~30min, Mycelium culture include following growth phase：
A, germination period：Inoculation was germination period in 15 days, and first three days temperature is maintained at 26~27 DEG C after inoculation, and air is relatively wet
Degree control is 60%~65%, and a day temperature is maintained at 25~26 DEG C from the 4th day to the 15th, relative air humidity control 60%~
65%；
B, the growth stage：16th day to 35 days, temperature was maintained at 23 DEG C~25 DEG C, relative air humidity control 55%~
65%；
C, the maturity period：After 35 days, mycelia enters the stage of ripeness, and temperature is maintained at 18~20 DEG C, relative air humidity control
65%~70%, stop water spray within 1~2 day before harvesting white fungus.
Embodiment 2
A kind of white fungus culture medium, including the raw material of following parts by weight are made：Cotton seed hulls 0.6kg；Wheat bran 0.15kg adds sulphur
Sour chromium aqueous solution, middle Cr after the addition of chromium sulfate aqueous solution3+Content be 12mg/kg.
The present invention also provides a kind of cultivating white fungus methods, include the following steps：Step 1, by siccative and water according to 1:1 weight
Amount is than being hybridly prepared into culture medium, and after mixing, hand of being subject to is micro- to hold agglomerating but not flowing water, be made moisture after bacterium bag be 60%~
65%, the siccative mixes after being crushed by the raw material of following weight：Cotton seed hulls 0.6kg；Wheat bran 0.15kg adds chromium sulfate
Cr in bacterium bag after aqueous solution3+Content be 12mg/kg；
Step 2, by the culture medium obtained by step 1 after mixing, bacteria stick, the weight of each bacteria stick is made after compacting pack
For 1.5kg, access white fungus strain carries out Mycelium culture after bacteria stick sterilizing, and Mycelium culture carries out under dark ventilation condition,
Daily ventilation 2~3 times, 15~30min, Mycelium culture include following growth phase every time：
A, germination period：Inoculation was germination period in 15 days, and first three days temperature is maintained at 26~27 DEG C after inoculation, and air is relatively wet
Degree control is 60%~65%, and a day temperature is maintained at 25~26 DEG C from the 4th day to the 15th, relative air humidity control 60%~
65%；
B, the growth stage：16th day to 35 days, temperature was maintained at 23 DEG C~25 DEG C, relative air humidity control 55%~
65%；
C, the maturity period：After 35 days, mycelia enters the stage of ripeness, and temperature is maintained at 18~20 DEG C, relative air humidity control
65%~70%, stop water spray within 1~2 day before harvesting white fungus.
Embodiment 3
A kind of white fungus culture medium, including the raw material of following parts by weight are made：Cotton seed hulls 0.58kg；Wheat bran 0.17kg, addition
Chromium nitrate aqueous solution, middle bacterium bag wheat bran Cr after chromium nitrate aqueous solution addition3+Content be 12mg/kg.The present invention also provides one kind
Cultivating white fungus method, includes the following steps：Step 1, by siccative and water according to 1:1 weight ratio is hybridly prepared into culture medium, mixes
After even, hand of being subject to is micro- to hold agglomerating but not flowing water, and moisture is 60%~65% after bacterium bag is made, and the siccative is by following weight
Raw material mixes after crushing：Cotton seed hulls 0.58kg；Wheat bran 0.17kg adds after chromium nitrate aqueous solution Cr in bacterium bag3+Content
For 12mg/kg；
Step 2, by the culture medium obtained by step 1 after mixing, bacteria stick is made after compacting pack, is accessed after bacteria stick sterilizing
White fungus strain carries out Mycelium culture, and Mycelium culture carries out under dark ventilation condition, daily ventilation 2~3 times, every time
15~30min, Mycelium culture include following growth phase：
A, germination period：Inoculation was germination period in 15 days, and first three days temperature is maintained at 26~27 DEG C after inoculation, and air is relatively wet
Degree control is 60%~65%, and a day temperature is maintained at 25~26 DEG C from the 4th day to the 15th, relative air humidity control 60%~
65%；
B, the growth stage：16th day to 35 days, temperature was maintained at 23 DEG C~25 DEG C, relative air humidity control 55%~
65%；
C, the maturity period：After 35 days, mycelia enters the stage of ripeness, and the criterion in maturity period is that fructification auricle is all stretched
Exhibition, growth stop, and do not have microtia stamen；Color is fresh white or cream colour, temperature are maintained at 18~20 DEG C, and relative air humidity control exists
65%~70%, stop water spray within 1~2 day before harvesting white fungus.
Experiment detection：
(1) for trying white fungus strain：
White white fungus (TR21), yellow white fungus (TR01).
(2) reagent is added
Chromium chloride (CrCl3, analyze pure, purity>99.5%)；
(3) compost and proportioning
Cotton seed hulls, wheat bran (8:2)；Material-water ratio about 1:1.
(4) experimental design
Using conventional plastic bag cultivation technology, in spice, first chromic nitrate is dissolved in spice water, then is fully mixed with culture material
Material bag is packed into after even, experiment 5 concentration gradients (CK, G1, G2, G3, G4) of setting, after sterilizing, each group bacteria stick Cr actual contents are such as
Table 1 shows.5 pitch-based spheres respectively set 3 repetitions in experiment in cultivation, each to repeat 20 bags, and 2 kinds amount to：5 (processing numbers) ×
60 (3 parallel bag numbers) × 2 (kind number)=600 bags.The experiment, altogether in 2017 in same factory cultivating white fungus,
3 wheels are repeated, as a result as table 1 shows.
Bacteria stick content (mg/kg) after chromium chloride addition in 1 cultivation matrix of table
N=3；
As it can be seen from table 1 chromic nitrate is added in cultivation matrix, when Cr contents reach 14.75 ± 1.05mg/ in bacterium bag
When kg, the significant raising of white fungus yield, respectively yellow white fungus TR01 output increaseds 22%, white white fungus TR21 output increaseds
15%.Attributional analysis the result shows that, no significant difference between 2 kinds, 5 processing.
In conclusion white fungus culture medium provided by the invention and cultivating white fungus method, through repetition test, the present invention is cultivating
Addition contains Cr in material3+Water soluble compound controls its a concentration of 12~18mg/kg, culture medium is made with cotton seed hulls and wheat bran, adopts
White fungus, the output increased of white fungus 15%~22%, the white fungus ear meat fertilizer of production are cultivated with culture medium prepared by inventive formulation
The yield of thickness, cultural method white fungus through the invention has obtained significant raising and has not been influenced on the quality of white fungus.
When chromium chloride additive amount is in optimum range, white fungus yield 15~22% can be significantly improved, and to white fungus quality
No conspicuousness influences.It removes the bacterium bag with miscellaneous bacteria in time during Mycelium culture, prevents other bacterium bags of living contaminants.
Example the above is only the implementation of the present invention is not intended to limit the scope of the invention, every to utilize this hair
Equivalents made by bright description are applied directly or indirectly in relevant technical field, are similarly included in this hair
In bright scope of patent protection.
Claims (7)
1. a kind of white fungus culture medium, which is characterized in that the raw material including following parts by weight is made：75~85 parts of cotton seed hulls, wheat bran
15~25 parts and contain Cr3+Water soluble compound；
It is described to contain Cr3+Cr in white fungus culture medium after water soluble compound addition3+Content be 12~18mg/kg.
2. white fungus culture medium according to claim 1, which is characterized in that the raw material including following parts by weight is made：Cottonseed
80 parts of shell, 20 parts of wheat bran and chromium chloride solution, Cr in white fungus culture medium after the chromium chloride solution addition3+Content be 14
~16mg/kg.
3. a kind of cultivating white fungus method, which is characterized in that include the following steps：
Step 1, by siccative and water according to 50~55：45~50 weight ratio is hybridly prepared into white fungus culture medium, the siccative by
The raw material of following parts by weight mixes after crushing：75~85 parts of cotton seed hulls, 15~25 parts of wheat bran and contain Cr3+Water-soluble chemical combination
Object, Cr in the white fungus culture medium3+Content be 12~18mg/kg；
Step 2, by the culture medium obtained by step 1 after mixing, bacteria stick is made after compacting pack, white fungus is accessed after bacteria stick sterilizing
Strain carries out Mycelium culture, and Mycelium culture carries out under dark ventilation condition, daily ventilation 2~3 times, every time 15~
30min, Mycelium culture include following growth phase：
A, germination period：Inoculation was germination period in 15 days, and first three days temperature is maintained at 26~27 DEG C after inoculation, relative air humidity control
System is 60%~65%, and a day temperature is maintained at 25~26 DEG C from the 4th day to the 15th, relative air humidity control 60%~
65%；
B, the growth stage：16th day to 35 days, temperature was maintained at 23 DEG C~25 DEG C, and relative air humidity is controlled 55%~65%；
C, the maturity period：After 35 days, mycelia enters stage of ripeness temperature and is maintained at 18~20 DEG C, and relative air humidity is controlled 65%
~70%, stop water spray within 1~2 day before harvesting white fungus.
4. cultivating white fungus method according to claim 3, which is characterized in that the step 1 is specially：Siccative and water are pressed
According to 1：1 weight ratio is hybridly prepared into white fungus culture medium, and the siccative mixes after being crushed by the raw material of following parts by weight：Cotton
80 parts of seed shell, 20 parts of wheat bran and chromium chloride solution, Cr in the white fungus culture medium3+Content be 14~16mg/kg.
5. cultivating white fungus method according to claim 3, which is characterized in that should be checked for during the Mycelium culture
Miscellaneous bacteria removes the bacterium bag with miscellaneous bacteria in time.
6. cultivating white fungus method according to claim 3, which is characterized in that the judgement to come to the ripening period in the step 2
Standard is that fructification auricle all stretches, and growth stops, and does not have microtia stamen；Color is fresh white or cream-coloured.
7. cultivating white fungus method according to claim 3, which is characterized in that the weight of each bacteria stick is in the step 2
1.5kg。
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Application publication date: 20180914 |