CN103371186B - A kind of bactericidal composition - Google Patents
A kind of bactericidal composition Download PDFInfo
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- CN103371186B CN103371186B CN201210108754.XA CN201210108754A CN103371186B CN 103371186 B CN103371186 B CN 103371186B CN 201210108754 A CN201210108754 A CN 201210108754A CN 103371186 B CN103371186 B CN 103371186B
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- antibiotic
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- pyrimidine nucleoside
- nucleoside class
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Abstract
The invention discloses a kind of bactericidal composition, the bactericidal composition containing active components A and active component B, active components A is selected from antibiotic in pyrimidine nucleoside class, and active component B is selected from any one compositions following：Polyoxin, probenazole, kasugarnycin, Isoprothiolane, and the weight of active components A and active component B is than for 1: 70～70: 1.The present composition can be used for preventing and treating powdery mildew in multiple kinds of crops, downy mildew, scab, banded sclerotial blight, gray mold, rust, epidemic disease, rust, spot defoliation, leaf mold, droop, soft rot, bacterial angular leaf spot, rice blast, false smut, damping-off, leaf blight, melasma, anthrax；Have that wide sterilization spectrum, dosage be little, resistance of rainwater washing against, the obvious feature of potentiation.
Description
Technical field
The invention belongs to technical field of pesticide, it is related to a kind of bactericidal composition in crop pest
Application.
Technical background
Antibiotic in pyrimidine nucleoside class (agricultural antiobiotic 120) is called：Pesticide corrosion 120；
Antimycoin 120；120 agricultural antibiotics.Antibiotic in pyrimidine nucleoside class is a kind of efficient, wide
Spectrum biofungicide, has prevention ＆ protection and interior suction treatment double effects；The protective ingredient of this product
One layer of fine and close polymer protective film can be formed on plant and fruit surface, to multiple pathogen
There are strong suppression and inhibition；Therapeutic component can be conducted by branch and reach inside fruit,
Directly hinder the synthesis of pathogenic proteins matter, lead to it dead.This product protection is fine and close, and absorbability is strong,
Continuous using being not likely to produce Drug resistance, even if using in rainy season, still can keep stronger interior
Inhale drug effect.
Polyoxin (polyoxin) chemical name：Peptide pyrimidine nucleoside, molecular formula：C17H25N5O14,
Polyoxin belongs to broad-spectrum antibiotics class, has preferable Uptake and translocation effect, and it acts on machine
System is the interference chitinous biosynthesiss of bacterium cell wall, and germ tube contacts after medicament with mycelium, office
Portion expands, ruptures, overflows cell inclusion, and is unable to normal development, leads to death, also
Suppression pathogenic bacteria produces spore and scab expansion effect, and this medicine does not have toxicity to animal, do not have medicine to plant
Evil.
Probenazole (probenazole) chemical name：3- allyloxy -1,2- benzisothia
Azoles -1,1- dioxide, molecular formula：C10H9NO3S, probenazole can extensively be protected and root
Except land for growing field crops effect, fruit tree, grassland, vegetable pathogenic bacteria；Slightly antimicrobial acivity in isolated test；
Process Oryza sativa L., promote Root Absorption, cover crop is not subject to Pyricularia oryzae and rice bacterial leaf spot pathogenic bacteria
Infect.
Kasugarnycin (kasugamycin) chemical name：[5- amino-2-methyl -6- (2,3,4,5,6-
Carbonyl cyclohexyl oxo) pyrans -3- base amino-a- IMDA], molecular formula：C14H25N3O9,
Kasugarnycin is agricultural antibiotic, has stronger absorbability, and this medicine mainly disturbs aminoacid
Metabolism esterase system, thus affecting the synthesis of protein, suppressing hyphal elongation and causing cell
Granulation, but spore germination is no affected.
Isoprothiolane (isoprothiolane) chemical name：Diisopropyl -1,3- dithiolane-a-
Phenyl malonic ester, molecular formula：C12H18O4S2, Isoprothiolane is systemic fungicide, to rice blast
Disease has specially good effect.Rice plant accumulates on leaf texture after absorbing medicament, specifically focuses on cob and branch
Stalk, thus suppressing pathogenic bacteria intrusion, hindering pathogenic bacteria lipid metabolism, suppressing pathogen growth, playing pre-
Prevent and therapeutical effect.
Content of the invention
Antibiotic in pyrimidine nucleoside class, polyoxin, probenazole, kasugarnycin, Isoprothiolane
Single dose life-time service may bring resistance to occur, drug effect is the problems such as decline, and proposed by the present invention kills
Bacteria composition contains active components A and active component B, and appropriate surfactant and load
Body.
A kind of bactericidal composition, containing active components A and active component B it is characterised in that：
Active components A and active component B weight than for 1 70～70 1, described active components A
Selected from antibiotic in pyrimidine nucleoside class, active component B be selected from polyoxin, probenazole,
One of kasugarnycin, Isoprothiolane kind, active components A and the preferred weight of active component B
Than for 1 35～35 1；It is more preferably the weight ratio of antibiotic in pyrimidine nucleoside class and polyoxin
For 1 10～10 1, the weight of antibiotic in pyrimidine nucleoside class and probenazole is than for 1
25～5 1, antibiotic in pyrimidine nucleoside class and kasugarnycin weight are than for 1 10～20 1, phonetic
The weight of pyridine nucleoside antibiotics and Isoprothiolane is than for 1 25～5 1.
The dosage form of described bactericidal composition is wettable powder or water dispersible granules or suspending agent
Or suspension emulsion or aqueous emulsion or microemulsion or microcapsule suspending agent or microcapsule suspension-suspending agent or water
Agent.
Described bactericidal composition is used for preventing and treating cereal crop, fruit tree, vegetable, ornamental plant
The purposes of upper disease.
Described disease includes：Powdery mildew, downy mildew, scab, banded sclerotial blight, gray mold,
Rust, epidemic disease, rust, spot defoliation, leaf mold, droop, soft rot, antibacterial
Property angular leaf spot, rice blast, false smut, damping-off, leaf blight, melasma, anthrax.
The weight of active components A and active component B is than for 1 70～70 1.Generally combine
In thing, the weight percentage of active component is the 0.5%～90% of gross weight, preferably
5%～80%.According to different preparation types, active component content scope is different.Logical
Often, liquid preparation contains by weight 0.5%～70% active substance, preferably 5%～
50%；Solid preparation contains by weight 5%～80% active substance, preferably 10%～
80%.
A kind of surfactant is at least contained, when being beneficial to administration in the bactericidal composition of the present invention
Dispersion in water for the active component.Surface-active contents are the 5%～30% of total formulation weight,
Balance of solid or liquid diluent.
Surfactant selected by the bactericidal composition of the present invention is those skilled in the art institute
Known：Can be selected from one or more of dispersant, wetting agent, binding agent or defoamer.
According to different dosage form, in preparation can also contain disintegrating agent known in those skilled in the art,
Antifreeze etc..
The bactericidal composition of the present invention can be by user before use through dilution or directly use.
It is prepared and can be prepared by common processing method known in those skilled in the art, will activity
After material is mixed with liquid flux or solid carrier, add surfactant such as dispersant, steady
Determine one or more of agent, wetting agent, binding agent, defoamer etc..
The bactericidal composition of the present invention, can be processed into acceptable dose on any pesticide on demand
Type, wherein more excellent dosage form is：Wettable powder, water dispersible granules, suspending agent, aqueous emulsion,
Microemulsion, microcapsule suspending agent, water preparation, can be made into microcapsule suspension-suspending agent, outstanding breast
Agent.
Compositionss are made and are comprised following constituent content during wettable powder：Active components A
1%～70%, active component B 1%～70%, dispersant 2%～10%, wetting agent 2%～10%,
Filler surplus.
Compositionss are made and are included following constituent content during water dispersible granules：Active components A 1%～
70%th, active component B 1%～70%, dispersant 3%～12%, wetting agent 1%～8%, disintegrate
Agent 1%～10%, binding agent 0～4%, filler surplus.
Compositionss are made and are included following constituent content during suspending agent：Active components A 0.5%～50%,
Active component B 0.5%～50%, dispersant 2%～10%, wetting agent 2%～10%, defoamer
0.01%～2%, thickening agent 0.05%～2%, antifreeze 0～8%, deionized water add to 100%.
Compositionss are made and are included following component and content during water preparation：Active components A 0.5%～40%,
Active component B 0.5%～40%, dispersant 2%～15%, wetting agent 1%-10%, antifreeze
0～8%, deionized water adds to 100%.
Compositionss are made and are comprised following constituent content during aqueous emulsion：Active components A 0.5%～50%,
Active component B 0.5%～50%, solvent 0～30%, emulsifying agent 1%～15%, antifreeze 0～8%,
Thickening agent 0.05%～2%, defoamer 0.01%～2%, deionized water supply surplus.
Compositionss are made and are comprised following constituent content during microemulsion：Active components A 0.5%～50%,
Active component B 0.5%～50%, solvent 0～30%, emulsifying agent 3%～20%, antifreeze 0～8%,
Thickening agent 0.05%～2%, defoamer 0.01%～2%, stabilizer 0～4%, deionized water is supplied
Surplus.
Compositionss are made and are included following constituent content during microcapsule suspending agent：Active components A
0.5%～50%, active component B 0.5%～50%, macromolecule cyst material 2%～10%, dispersion
Agent 1%～10%, organic solvent 0～10%, emulsifying agent 1%～7%, pH adjusting agent 0.1%～5%,
Deionized water adds to 100%.
If comprising following weight portion when making microcapsule suspension-suspendinagent agent, suspension emulsion：
Compositionss are made and are included following constituent content during microcapsule suspension-suspendinagent agent：Active components A
0.5%～50%, active component B 0.5%～50%, macromolecule cyst material 2%～12%, surface
Activating agent 3%～15%, organic solvent 1%～15%, emulsifying agent 1%～6%, defoamer 0.01%
～2%, thickening agent 0.05%～2%, pH adjusting agent 0.1%～5%, deionized water add to 100%；
Compositionss are made and are included following constituent content during suspension emulsion：Active components A 0.5%～50%, work
Property composition B 0.5%～50%, dispersant 2%～10%, defoamer 0.01%～2%, solvent 0～15%,
Thickening agent 0.05%～2%, emulsifying agent 2%～12%, antifreeze 0～8%, stabilizer 0～3%,
Deionized water adds to 100%.
The wettable powder the key technical indexes of the present invention：
The water dispersible granules the key technical indexes of the present invention：
The suspending agent the key technical indexes of the present invention：
The water preparation the key technical indexes of the present invention：
The aqueous emulsion the key technical indexes of the present invention：
The microemulsion the key technical indexes of the present invention：
The microcapsule suspending agent the key technical indexes of the present invention：
The microcapsule suspension-suspendinagent agent the key technical indexes of the present invention：
The suspension emulsion the key technical indexes of the present invention：
It is an advantage of the current invention that：
(1) antibiotic in pyrimidine nucleoside class, polyoxin, probenazole, kasugarnycin, rice
After pestilence spirit is compounding, there is obvious potentiation and lasting effect；(2) to cereal crop, fruit tree,
Powdery mildew on vegetable, ornamental plant, downy mildew, scab, banded sclerotial blight, gray mold, rust
Disease, epidemic disease, rust, spot defoliation, leaf mold, droop, soft rot, bacillary
Angular leaf spot, rice blast, false smut, damping-off, leaf blight, melasma, anthrax etc. all have
Specially good effect；(3) decrease pesticide usage amount, reduce residual quantity on crop for the pesticide, mitigate
Environmental pollution；(4) to person poultry safety, Environmental compatibility is good；And preparation adhesion strength strengthens,
Resistance of rainwater washing against.
Specific embodiment
With reference to embodiment to further instruction of the present invention, the percentage ratio in embodiment is
Percentage by weight, but the invention is not limited in this.
Application Example one
Example 1～12 wettable powder
By antibiotic in pyrimidine nucleoside class, active component B, dispersant, wetting agent, filler mixed
Close mix homogeneously in cylinder, remix uniformly after pulverizing through jet mill, you can the prepared present invention
Described wettable powder product, is specifically shown in Table 1.
The each component of table 1 embodiment 1～12 and weight portion
Polyoxin, probenazole, kasugarnycin, Isoprothiolane in table are exchanged, can be obtained
Novel formulation.
Example 13～24 water dispersible granules
By antibiotic in pyrimidine nucleoside class, active component B, dispersant, wetting agent, disintegrating agent,
Filler etc. obtains the particle diameter of needs together through comminution by gas stream, adds other auxiliary agent such as binding agent,
Obtain granulation materials.Item is quantitatively sent in fluidized bed granulating dryer through pelletizing and being dried
Afterwards, you can of the present invention water dispersible granules product is obtained, is specifically shown in Table 2.
The each component of table 2 embodiment 13～24 and weight portion
Polyoxin, probenazole, kasugarnycin, Isoprothiolane in table are exchanged, can be obtained
Novel formulation.
Example 25～34 suspending agent
By dispersant, wetting agent, defoamer, thickening agent, antifreeze, mix through high speed shear
Close uniformly, add antibiotic in pyrimidine nucleoside class, active component B, ball milling 2～3 in ball mill
Hour, make diameter of particle all below 5 μm, surplus deionized water is supplied, you can system
Obtain suspending agent product of the present invention, be specifically shown in Table 3-4.
The each component of table 3 embodiment 25～30 and weight portion
The each component of table 4 embodiment 31～34 and weight portion
Polyoxin, probenazole, kasugarnycin, Isoprothiolane in table are exchanged, can be obtained
Novel formulation.
Example 35～39 aqueous emulsion
Antibiotic in pyrimidine nucleoside class, active component B, solvent, emulsifying agent are added together, make
It is dissolved into homogeneous oil phase；Deionized water, antifreeze, thickening agent, defoamer are mixed,
Become homogeneous aqueous phase.Under high velocity agitation, aqueous phase is added oil phase, surplus deionized water is supplied；
Can be prepared by aqueous emulsion product of the present invention, be specifically shown in Table 5.
The each component of table 5 embodiment 35～39 and weight portion
Polyoxin, probenazole, kasugarnycin, Isoprothiolane in table are exchanged, can be obtained
Novel formulation.
Example 40～44 microemulsion
By antibiotic in pyrimidine nucleoside class, active component B, solvent, emulsifying agent, antifreeze, increasing
Thick dose, defoamer be sufficiently mixed uniformly transparent oil phase, be slowly added into deionization under agitation
Water, forms Water in Oil emulsion, more agitated heating, is allowed to rapid phase inversion and becomes oil-in-water type,
It is cooled to room temperature and makes up to balance, through filtering, surplus deionized water is supplied；Can be prepared by this
The described microemulsion product of invention, is specifically shown in Table 6.
The each component of table 6 embodiment 40～44 and weight portion
Polyoxin, probenazole, kasugarnycin, Isoprothiolane in table are exchanged, can be obtained
Novel formulation.
Example 45-48 microcapsule suspending agent
By antibiotic in pyrimidine nucleoside class, active component B, macromolecule cyst material, solvent mixing,
Make to be dissolved into homogeneous oil phase, in a shear condition, oil phase is added to containing emulsifying agent, pH
In regulator, the aqueous phase solution of dispersant, surplus deionized water is supplied, and bi-material is in oil
Water termination reacts, and forms high score ascus wall, makes the present composition finely disseminated micro-
Capsule suspending agent product, is specifically shown in Table 7-8.
Table 7 embodiment 45,46 each components and weight portion
Table 8 embodiment 47,48 each components and weight portion
Example 49～53 water preparation
By antibiotic in pyrimidine nucleoside class, active component B, dispersant, wetting agent, antifreeze warp
Cross high speed shear-mixed uniformly, deionized water supplies surplus, of the present invention water preparation is obtained
Product.Specifically it is shown in Table 9.
The each component of table 9 example 49～53 and content
Polyoxin, kasugarnycin in table are exchanged, novel formulation can be obtained.
The embodiment of the present invention is the method being combined using Toxicity Determination and field test.
The embodiment of the present invention is the method being combined using Toxicity Determination and field test.First pass through
Toxicity Determination, the synergy ratio (SR) after clear and definite two kinds of medicaments are compounding by a certain percentage,
SR ＜ 0.5 is antagonism, and 0.5≤SR≤1.5 are summation action, and SR ＞ 1.5 is potentiation,
On this basis, then carry out field test.
Antibiotic in pyrimidine nucleoside class is compounded to crop pest Toxicity Determination with active component B.
Determine that after each medicament effective inhibition concentration scope, each medicament is divided by active constituent content through prerun
Do not set 5 dosage to process, if clear water comparison.Reference《Farm-chemical indoor determination test rule
Antibacterial》Carry out, the virulence to crop pathogenic bacteria for the medicament is measured using mycelial growth rate method.72h
Measure colony diameter with crossing method afterwards, calculate each process net growth, mycelial growth suppression
Rate.
Net growth (mm)=measurement colony diameter -5
Mycelial growth inhibition rate is converted into probit value (y), liquor strength (μ g/mL) is changed
Become logarithm value (x), virulence regression equation (y=a+bx) is tried to achieve with method of least square, and thus
Calculate the EC of every kind of medicament50Value.Two medicament different ratio are calculated according to Wadley method simultaneously
Synergy ratio (SR), SR ＜ 0.5 is antagonism, and 0.5≤SR≤1.5 are summation action,
SR ＞ 1.5 is potentiation.Computing formula is as follows：
Wherein：A, b are respectively active component antibiotic in pyrimidine nucleoside class and active component B in group
Shared ratio in conjunction；
A is antibiotic in pyrimidine nucleoside class；
B is one of polyoxin, kasugarnycin, probenazole, Isoprothiolane.
Implementation of an application example two：Antibiotic in pyrimidine nucleoside class and polyoxin compound indoor to tomato late blight
Toxicity test.
Test medicine is provided by Shaanxi Welch Crop Protection Co., Ltd..
EXPERIMENTAL DESIGN：Determine antibiotic in pyrimidine nucleoside class, polyoxin active compound through pilot study
And effective inhibition concentration scope of the two different ratio mixture.
Toxicity test result
Table 10 pyridine nucleoside antibiotics, polyoxin and its compounding toxicity test analysis of results table to tomato late blight
As shown in Table 10, antibiotic in pyrimidine nucleoside class, the EC to tomato late blight for the polyoxin50
It is respectively 3.97mg/L and 4.08mg/L, and proportioning is 70：1 to 1：When 70, potentiation
Ratio SR is all higher than 1.5, illustrates antibiotic in pyrimidine nucleoside class and polyoxin 70 1
To in the range of 1 70, mixture all shows potentiation, especially when the proportioning of the two is 1：
10 to 10：When 1, potentiation becomes apparent from projecting, and synergy ratio is all higher than 2.10, its
In when antibiotic in pyrimidine nucleoside class and cymoxanil weight more maximum than synergy ratio SR during for 11,
Potentiation is the most obvious, and test finds, when joining of antibiotic in pyrimidine nucleoside class and polyoxin
Than 10：1、9：1、8：1、7：1、6：1、5：1、4：1、3：1、2：1、
1：1、1：2、1：3、1：4、1：5、1：6、1：7、1：8、1：9、1：10
When to powdery mildew, downy mildew, scab, banded sclerotial blight, gray mold, rust, epidemic disease, red star
Disease, spot defoliation, leaf mold, droop, soft rot, angular leaf spot, rice blast, rice are bent
Substantially, synergy ratio is all big for disease, damping-off, leaf blight, melasma, anthrax potentiation
In 1.5.
Implementation of an application example three：Antibiotic in pyrimidine nucleoside class is compounded to Oryza sativa L. leaf blight room with probenazole
Interior toxicity test.
Test medicine is provided by Shaanxi Welch Crop Protection Co., Ltd..
EXPERIMENTAL DESIGN：Determine that antibiotic in pyrimidine nucleoside class, probenazole are former through pilot study
Medicine and effective inhibition concentration scope of the two different ratio mixture.
Toxicity test result
Table 11 pyridine nucleoside antibiotics, probenazole and its compounding toxicity test analysis of results table to Oryza sativa L. leaf blight
As shown in Table 11, antibiotic in pyrimidine nucleoside class, probenazole are to Oryza sativa L. leaf blight
EC50It is respectively 3.88mg/L and 19.36mg/L, and proportioning is 70：1 to 1：When 70,
Synergy ratio SR is all higher than 1.5, and antibiotic in pyrimidine nucleoside class and probenazole are described
In the range of 70 1 to 1 70, mixture all shows potentiation, especially joining when the two
Than 1：25 to 5：When 1, potentiation becomes apparent from projecting, and synergy ratio is all higher than 2.30,
Wherein when antibiotic in pyrimidine nucleoside class and probenazole weight are than synergy ratio SR during for 15
Maximum, potentiation is the most obvious, and test finds, when antibiotic in pyrimidine nucleoside class and phenyl allyloxy
The proportioning of thiazole is 5：1、4：1、3：1、2：1、1：1、1：2、1：3、1：4、
1：5、1：6、1：7、1：8、1：9、1：10、1：15、1：20、1：When 25
To powdery mildew, downy mildew, scab, banded sclerotial blight, gray mold, rust, epidemic disease, rust,
Spot defoliation, leaf mold, droop, soft rot, angular leaf spot, rice blast, false smut,
Substantially, synergy ratio is all higher than for damping-off, leaf blight, melasma, anthrax potentiation
1.5.
Implementation of an application example four：Antibiotic in pyrimidine nucleoside class and kasugarnycin compound indoor to Fructus Cucumidis sativi leaf mold
Toxicity test.
Test medicine is provided by Shaanxi Welch Crop Protection Co., Ltd..
EXPERIMENTAL DESIGN：Determine antibiotic in pyrimidine nucleoside class, kasugarnycin active compound through pilot study
And effective inhibition concentration scope of the two different ratio mixture.
Toxicity test result
Table 12 pyridine nucleoside antibiotics, kasugarnycin and its compounding toxicity test analysis of results table to Fructus Cucumidis sativi leaf mold
As shown in Table 12, antibiotic in pyrimidine nucleoside class, the EC to Fructus Cucumidis sativi leaf mold for the kasugarnycin50
It is respectively 4.12mg/L and 2.04mg/L, and proportioning is 70：1 to 1：When 70, potentiation
Ratio SR is all higher than 1.5, illustrates antibiotic in pyrimidine nucleoside class and kasugarnycin 70 1
To in the range of 1 70, mixture all shows potentiation, especially when the proportioning of the two is 1：
10 to 20：When 1, potentiation becomes apparent from projecting, and synergy ratio is all higher than 2.00, its
In when antibiotic in pyrimidine nucleoside class with kasugarnycin weight than synergy ratio SR during for 21
Greatly, potentiation is the most obvious, and test finds, when antibiotic in pyrimidine nucleoside class and kasugarnycin
Proportioning 20：1、15：1、10：1、9：1、8：1、7：1、6：1、5：1、
4：1、3：1、2：1、1：1、1：2、1：3、1：4、1：5、1：6、1：7、
1：8、1：9、1：When 10 to powdery mildew, downy mildew, scab, banded sclerotial blight, gray mold,
Rust, epidemic disease, rust, spot defoliation, leaf mold, droop, soft rot, angle speckle
Disease, rice blast, false smut, damping-off, leaf blight, melasma, anthrax potentiation are bright
Aobvious, synergy ratio is all higher than 1.5.
Implementation of an application example five：Antibiotic in pyrimidine nucleoside class and Isoprothiolane compound the indoor poison to rice blast
Power measures.
Test medicine is provided by Shaanxi Welch Crop Protection Co., Ltd..
EXPERIMENTAL DESIGN：Through pilot study determine antibiotic in pyrimidine nucleoside class, isoprothiolane and
Effective inhibition concentration scope of the two different ratio mixture.
Toxicity test result
Table 13 pyridine nucleoside antibiotics, Isoprothiolane and its compounding toxicity test analysis of results table to rice blast
As shown in Table 13, antibiotic in pyrimidine nucleoside class, the EC to rice blast for the Isoprothiolane50
It is respectively 3.76mg/L and 18.68mg/L, and proportioning is 70：1 to 1：When 70, increase
Effect ratio SR is all higher than 1.5, illustrate antibiotic in pyrimidine nucleoside class and Isoprothiolane 70 1
To in the range of 1 70, mixture all shows potentiation, especially when the proportioning of the two is 1：
25 to 5：When 1, potentiation becomes apparent from projecting, and synergy ratio is all higher than 2.10, wherein
When antibiotic in pyrimidine nucleoside class is more maximum than synergy ratio SR during for 15 with Isoprothiolane weight,
Potentiation is the most obvious, and test finds, when the proportioning of antibiotic in pyrimidine nucleoside class and Isoprothiolane
5：1、4：1、3：1、2：1、1：1、1：2、1：3、1：4、1：5、1：
6、1：7、1：8、1：9、1：10、1：15、1：20、1：When 25 to powdery mildew,
Downy mildew, scab, banded sclerotial blight, gray mold, rust, epidemic disease, rust, speckle fallen leaves
Disease, leaf mold, droop, soft rot, angular leaf spot, rice blast, false smut, damping-off,
Substantially, synergy ratio is all higher than 1.5 for leaf blight, melasma, anthrax potentiation.
Application Example Elmidone nucleoside antibiotics and active component B compound preventing and treating Fructus Vitis viniferae downy mildew
The sick test of pesticide effectiveness
, in Xi'an City, Shanxi Province Baqiao District suburb, test medicine is by Shaanxi Wei Erqi for this experimental establishment
Crop protection company limited provides, comparison medicament 4% antibiotic in pyrimidine nucleoside class water preparation (commercial),
3% Wettable polyoxin powder (commercial), 8% probenazole granule (commercial), 2%
Kasugarnycin wettable powder (commercial), 40% rice blast missible oil (commercial).
Investigate the downy mildew of garpe state of an illness before medicine, in the first time dispenser of state of an illness initial stage, apply within every 10 days
Medicine once, is total to dispenser 2 times.7 days after second dispenser, investigate disease respectively within 14 days, 30 days
Feelings index simultaneously calculates preventive effect.Experimental result is as follows：
Table 14 antibiotic in pyrimidine nucleoside class and active component B prevent and treat the downy mildew of garpe test of pesticide effectiveness
As can be seen from Table 14, antibiotic in pyrimidine nucleoside class and polyoxin, probenazole,
Kasugarnycin, Isoprothiolane can effectively prevent and treat downy mildew of garpe after compounding, and prevention effect is superior to list
The preventive effect of agent, and efficiency time is long；In test scope of medication, target crop is had no adverse effects.
Application Example seven antibiotic in pyrimidine nucleoside class and active component B compound preventing and treating apple tree speckle
The point defoliation test of pesticide effectiveness
, in Shaanxi Province's Weinan City Baishui County, test medicine is by Shaanxi Wei Erqi crop for this experimental establishment
Protection company limited provides, comparison medicament 4% antibiotic in pyrimidine nucleoside class water preparation (commercial), 3%
Wettable polyoxin powder (commercial), 8% probenazole granule (commercial), 2% spring
Thunder mycin wettable powder (commercial), 40% rice blast missible oil (commercial).
The apple peels and cores state of an illness is investigated, in the first time dispenser of state of an illness initial stage, often before medicine
Dispenser in 10 days once, is total to dispenser 2 times.7 days after second dispenser, 14 days, 30 days respectively
Investigation disease index simultaneously calculates preventive effect.Experimental result is as follows：
Table 15 antibiotic in pyrimidine nucleoside class prevents and treats the apple peels and cores test of pesticide effectiveness with active component B
As can be seen from Table 15, antibiotic in pyrimidine nucleoside class and polyoxin, probenazole,
Energy effectively preventing and treating apple-tree spot defoliation after kasugarnycin, Isoprothiolane are compounding, prevention effect is equal
Better than the preventive effect of single dose, and efficiency time is long；In test scope of medication to target crop invariably
Good impact.
Application Example eight antibiotic in pyrimidine nucleoside class and active component B compound preventing and treating Oryza sativa L. stand withered
The sick test of pesticide effectiveness
This experimental establishment is protected by Shaanxi Wei Erqi crop in Shaanxi Province Hanzhong suburban area, test medicine
Shield company limited provides, comparison medicament 4% antibiotic in pyrimidine nucleoside class water preparation (commercial), 3%
Wettable polyoxin powder (commercial), 8% probenazole granule (commercial), 2% spring
Thunder mycin wettable powder (commercial), 40% rice blast missible oil (commercial).
Investigate the rice seedling blight state of an illness before medicine, in the first time dispenser of state of an illness initial stage, apply within every 10 days
Medicine once, is total to dispenser 2 times.7 days after second dispenser, investigate disease respectively within 14 days, 30 days
Feelings index simultaneously calculates preventive effect.Experimental result is as follows：
Table 16 antibiotic in pyrimidine nucleoside class prevents and treats the rice seedling blight test of pesticide effectiveness with active component B
As can be seen from Table 16, antibiotic in pyrimidine nucleoside class and polyoxin, probenazole,
Kasugarnycin, Isoprothiolane can effectively prevent and treat rice seedling blight after compounding, and prevention effect is superior to list
The preventive effect of agent, and efficiency time is long；In test scope of medication, target crop is had no adverse effects.
Application Example nine antibiotic in pyrimidine nucleoside class and active component B compound preventing and treating Flos Rosae Rugosae white lead
The sick test of pesticide effectiveness
This experimental establishment is protected by Shaanxi Wei Erqi crop in Yunnan Province Kunming suburban area, test medicine
Shield company limited provides, comparison medicament 4% antibiotic in pyrimidine nucleoside class water preparation (commercial), 3%
Wettable polyoxin powder (commercial), 8% probenazole granule (commercial), 2% spring
Thunder mycin wettable powder (commercial), 40% rice blast missible oil (commercial).
Investigate the Flos Rosae Rugosae powdery mildew state of an illness before medicine, in the first time dispenser of state of an illness initial stage, apply within every 10 days
Medicine once, is total to dispenser 2 times.7 days after second dispenser, investigate disease respectively within 14 days, 30 days
Feelings index simultaneously calculates preventive effect.Experimental result is as follows：
Table 17 antibiotic in pyrimidine nucleoside class prevents and treats the Flos Rosae Rugosae powdery mildew test of pesticide effectiveness with active component B
As can be seen from Table 17, antibiotic in pyrimidine nucleoside class and polyoxin, probenazole,
Kasugarnycin, Isoprothiolane can effectively prevent and treat Flos Rosae Rugosae powdery mildew after compounding, and prevention effect is superior to list
The preventive effect of agent, and efficiency time is long；In test scope of medication, target crop is had no adverse effects.
Application Example ten antibiotic in pyrimidine nucleoside class is compounded with active component B and prevents and treats cucumber anthracnose
The sick test of pesticide effectiveness
, in Xi'an City, Shanxi Province Baqiao District, test medicine is by Shaanxi Wei Erqi crop for this experimental establishment
Protection company limited provides, comparison medicament 4% antibiotic in pyrimidine nucleoside class water preparation (commercial), 3%
Wettable polyoxin powder (commercial), 8% probenazole granule (commercial), 2% spring
Thunder mycin wettable powder (commercial), 40% rice blast missible oil (commercial).
Investigate the cucumber anthracnose state of an illness before medicine, in the first time dispenser of state of an illness initial stage, apply within every 10 days
Medicine once, is total to dispenser 2 times.7 days after second dispenser, investigate disease respectively within 14 days, 30 days
Feelings index simultaneously calculates preventive effect.Experimental result is as follows：
Table 18 antibiotic in pyrimidine nucleoside class prevents and treats the cucumber anthracnose test of pesticide effectiveness with active component B
As can be seen from Table 18, antibiotic in pyrimidine nucleoside class and polyoxin, probenazole,
Kasugarnycin, Isoprothiolane can effectively prevent and treat cucumber anthracnose after compounding, and prevention effect is superior to list
The preventive effect of agent, and efficiency time is long；In test scope of medication, target crop is had no adverse effects.
Test through different places throughout the country draws afterwards, antibiotic in pyrimidine nucleoside class with many
Antimycin, probenazole, kasugarnycin, Isoprothiolane compound and make wettable powder, moisture
Shot agent, suspending agent, suspension emulsion, aqueous emulsion, microemulsion, microcapsule suspending agent, microcapsule suspension
After-suspending agent, water preparation to the powdery mildew of all kinds of crops, downy mildew, scab, banded sclerotial blight,
Gray mold, rust, epidemic disease, rust, spot defoliation, leaf mold, droop, soft corruption
Disease, bacterial angular leaf spot, rice blast, false smut, damping-off, leaf blight, melasma, charcoal
, all more than 95%, better than single dose preventive effect, potentiation is obvious for the preventive effect of the Common Diseases such as cellulitis disease.
Claims (6)
1. a kind of bactericidal composition, effective active composition be active components A and active component B it is characterised in that：For 1 70 ~ 70 1, described active components A is antibiotic in pyrimidine nucleoside class to active components A and active component B weight ratio, and active component B is probenazole.
2. a kind of bactericidal composition according to claim 1 it is characterised in that：The weight of active components A and active component B is than for 1 35 ~ 35 1.
3. a kind of bactericidal composition according to claim 2 it is characterised in that：The weight of antibiotic in pyrimidine nucleoside class and probenazole is than for 1 25 ~ 51.
4. a kind of bactericidal composition according to any one of claim 1 to 3 it is characterised in that：Compositionss make wettable powder, water dispersible granules, suspending agent, suspension emulsion, aqueous emulsion, microemulsion, microcapsule suspending agent, microcapsule suspension-suspending agent.
5. a kind of bactericidal composition described in claim 4 is used for preventing and treating the purposes of disease on crops.
6. purposes according to claim 5, it is characterized in that, described disease is powdery mildew, downy mildew, scab, banded sclerotial blight, gray mold, rust, epidemic disease, rust, spot defoliation, leaf mold, droop, soft rot, bacterial angular leaf spot, rice blast, false smut, damping-off, leaf blight, melasma, anthrax.
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| WO2009090038A1 (en) * | 2008-01-16 | 2009-07-23 | Syngenta Participations Ag | Fungicidal compositions |
| CN101647441A (en) * | 2009-07-31 | 2010-02-17 | 深圳诺普信农化股份有限公司 | Sterilization combination containing azoxystrobin and application thereof |
| CN101669524A (en) * | 2009-10-16 | 2010-03-17 | 深圳诺普信农化股份有限公司 | Bactericide composition with tetraconazole |
| CN101669474A (en) * | 2009-08-23 | 2010-03-17 | 山东兆丰年生物科技有限公司 | Bactericidal composition containing kresoxim-methyl phenylate |
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| WO2009090038A1 (en) * | 2008-01-16 | 2009-07-23 | Syngenta Participations Ag | Fungicidal compositions |
| CN101647441A (en) * | 2009-07-31 | 2010-02-17 | 深圳诺普信农化股份有限公司 | Sterilization combination containing azoxystrobin and application thereof |
| CN101669474A (en) * | 2009-08-23 | 2010-03-17 | 山东兆丰年生物科技有限公司 | Bactericidal composition containing kresoxim-methyl phenylate |
| CN101669524A (en) * | 2009-10-16 | 2010-03-17 | 深圳诺普信农化股份有限公司 | Bactericide composition with tetraconazole |
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