CN103348915A - Method for strong seedling and rooting culture of strawberries - Google Patents
Method for strong seedling and rooting culture of strawberries Download PDFInfo
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- CN103348915A CN103348915A CN2013102972836A CN201310297283A CN103348915A CN 103348915 A CN103348915 A CN 103348915A CN 2013102972836 A CN2013102972836 A CN 2013102972836A CN 201310297283 A CN201310297283 A CN 201310297283A CN 103348915 A CN103348915 A CN 103348915A
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- strengthening seedling
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Abstract
The invention discloses a method for strong seedling and rooting culture of strawberries, which includes the following steps: firstly, strong seedling and rooting culture: a multiple shoot with a prophyll and 0.3-0.5 cm in height and well in growth and development is cut into a single plant and transplanted to a strong seedling and rooting culture medium for rooting culture, and transplanting is carried out after the plant is 3-5 cm in height; secondly, transplantation: the plant is firstly transplanted to a culture room and grows for 30-35 d, then is transplanted in a plastic-covered tunnel with a shading net, after a new root grows and the plant sprouts, watering frequency is gradually reduced, and the plant is transplanted to a flowerpot for ordinary management. The method for strong seedling and rooting culture of strawberries, provided by the invention, improves the survival rate of transplanted plants by 90% above through supporting measures such as improvement on minimal medium and ingredients and adjustment in culture conditions, enables seedlings to grow healthy, effectively solves the problem of genetical characterization decline of fine seedlings, effectively improves yield and quality of strawberries and enhances the economic value of strawberries at the same time.
Description
Technical field
The present invention relates to a kind of strawberry strengthening seedling and rooting cultural method, belong to the botany technical field.
Background technology
Strawberry production, its strengthening seedling and rooting process is very crucial, need to be without damage by disease and insect, and blade quantity is reasonable, color is bud green, rhizome is flourishing, even thickness, and traditional strawberry suffers damage by disease and insect easily in the strong plantlets and rootage stage, cause its root system degeneration, affected survival rate and Yield of Strawberry, therefore, be badly in need of a kind of method of cultivation of organizing it is organized the cultivation strong plantlets and rootage.
Summary of the invention
In order to overcome the deficiencies in the prior art, the object of the present invention is to provide a kind of problem that can effectively solve the degeneration of strawberry seedling germplasm, improve the strawberry strengthening seedling and rooting cultural method of survival rate and output.
In order to realize the foregoing invention purpose, the present invention is achieved through the following technical solutions:
A kind of strawberry strengthening seedling and rooting cultural method is characterized in that, may further comprise the steps:
(1) strengthening seedling and rooting is cultivated:
The Multiple Buds with a prophyll of healthy and strong length behind the 0.3-0.5cm that grow is cut into individual plant to be transferred on the strengthening seedling and rooting medium, keeping temperature is 20-25 ℃, light application time is 15-20h, cultivate 5-10 week, guarantee that the cauline leaf growth is normal, root growth is healthy and strong, when plant length arrives 3-5cm, transplants;
(2) transplant:
With the described plantlet of transplant of step (1) in culturing room, with the residual medium of clear water flush away base portion, and the maintenance indoor temperature is 20-25 ℃, behind the 30-35d, be transplanted in the shading screen plastic tunnel of obscurity 60-70%, under temperature is 20-25 ℃ condition, be transplanted in the dish of cave, and keep relative moisture more than 80%, behind the 5-10d, after new root sprouting eruption, reduce gradually irrigation times and be transplanted to flowerpot and carry out Routine Management.
Further, described strawberry strengthening seedling and rooting medium comprises improvement MS+0.2～0.3mgL
-1IBA+0.01～0.03mgL
-1NAA+0.5～0.8mg indolebutyric acid+0.2～0.4 gL
-1Active carbon+4.0～5.0gL
-1Agar+10gL
-1Sucrose, and the pH value is 5.2～5.8.
Wherein, described improvement MS comprises grand nutrition element, micronutrient element and organic reagent,
The component of described grand nutrition element and its corresponding concentration are: potassium nitrate 1800 mg/L; Ammonium sulfate 1880 mg/L; Calcium chloride dihydrate 360 mg/L; Anhydrous potassium dihydrogenphosphate 150mg/L; Disodium ethylene diamine tetraacetate 32.5 mg/L; Ferrous sulfate heptahydrate 25.3 mg/L,
The component of described micronutrient element and its corresponding concentration are: four water manganese sulphates, 18.5 mg/L; Zinc sulphate 9.5mg/L; Boric acid 7.5 mg/L; Potassium iodide 1.5mg/L; Sodium molybdate 0.5mg/L; Cobalt chloride 0.03 mg/L,
And the component of described organic reagent and its corresponding concentration are: thiamine hydrochloride 15.0 mg/L; Nicotinic acid 2.0 mg/L; Puridoxine hydrochloride 2.0mg/L; Inositol 80.0 mg/L.
The invention has the beneficial effects as follows: strawberry strengthening seedling and rooting cultural method of the present invention is by improveing the supplementary measures such as minimal medium and composition, adjustment condition of culture, so that the plantlet of transplant survival rate can reach more than 90%, seedling early growth is healthy and strong, can effectively solve good seed germplasm degenerate problem, simultaneously Effective Raise Yield of Strawberry and quality, strengthened its economic worth.
Embodiment
Below in conjunction with specific embodiment, describe the specific embodiment of the present invention in detail:
In the present embodiment, the improvement MS that adopts among all embodiment comprises grand nutrition element, micronutrient element and organic reagent,
And the component of described grand nutrition element and its corresponding concentration are: potassium nitrate 1800 mg/L; Ammonium sulfate 1880 mg/L; Calcium chloride dihydrate 360 mg/L; Anhydrous potassium dihydrogenphosphate 150mg/L; Disodium ethylene diamine tetraacetate 32.5 mg/L; Ferrous sulfate heptahydrate 25.3 mg/L,
The component of described micronutrient element and its corresponding concentration are: four water manganese sulphates, 18.5 mg/L; Zinc sulphate 9.5mg/L; Boric acid 7.5 mg/L; Potassium iodide 1.5mg/L; Sodium molybdate 0.5mg/L; Cobalt chloride 0.03 mg/L,
And the component of described organic reagent and its corresponding concentration are: thiamine hydrochloride 15.0 mg/L; Nicotinic acid 2.0 mg/L; Puridoxine hydrochloride 2.0mg/L; Inositol 80.0 mg/L.
Embodiment 1
The strengthening seedling and rooting cultural method of strawberry of the present invention is specially:
(1) strengthening seedling and rooting is cultivated:
The Multiple Buds with a prophyll of healthy and strong length behind the 0.3-0.5cm that grow is cut into individual plant is transferred on the strengthening seedling and rooting medium, described strawberry strengthening seedling and rooting medium comprises improvement MS+0.2mgL
-1IBA+0.01mgL
-1NAA+0.8mg indolebutyric acid+0.2 gL
-1Active carbon+5.0gL
-1Agar+10gL
-1Sucrose, and the pH value is 5.2, and to keep temperature be 20-25 ℃, light application time is 15-20h, cultivates for 5 weeks, and cauline leaf is grown normal, and root growth is healthy and strong, and plant is long during to 3-5cm, transplants;
(2) transplant:
With the described plantlet of transplant of step (1) in culturing room, with the residual medium of clear water flush away base portion, and the maintenance indoor temperature is 20-25 ℃, behind the 30d, be transplanted in the shading screen plastic tunnel of obscurity 60-70%, under temperature is 20-25 ℃ condition, be transplanted in the dish of cave, and keep relative moisture more than 80%, behind the 5d, after new root sprouting eruption, reduce gradually irrigation times and be transplanted to flowerpot and carry out Routine Management.
Embodiment 2
The strengthening seedling and rooting cultural method of strawberry of the present invention is specially:
(1) strengthening seedling and rooting is cultivated:
The Multiple Buds with a prophyll of healthy and strong length behind the 0.3-0.5cm that grow is cut into individual plant is transferred on the strengthening seedling and rooting medium, described strawberry strengthening seedling and rooting medium comprises improvement MS+0.3mgL
-1IBA+0.03mgL
-1NAA+0.5mg indolebutyric acid+0.4 gL
-1Active carbon+4.0gL
-1Agar+10gL
-1Sucrose, and the pH value is 5.5, and to keep temperature be 20-25 ℃, light application time is 15-20h, cultivated for 10 weeks after, cauline leaf is grown normal, root growth is healthy and strong, plant is long during to 3-5cm, transplants;
(2) transplant:
With the described plantlet of transplant of step (1) in culturing room, with the residual medium of clear water flush away base portion, and the maintenance indoor temperature is 20-25 ℃, behind the 35d, be transplanted in the shading screen plastic tunnel of obscurity 60-70%, under temperature is 20-25 ℃ condition, be transplanted in the dish of cave, and keep relative moisture more than 80%, behind the 10d, after new root sprouting eruption, reduce gradually irrigation times and be transplanted to flowerpot and carry out Routine Management.
Embodiment 3
The strengthening seedling and rooting cultural method of strawberry of the present invention is specially:
(1) strengthening seedling and rooting is cultivated:
The Multiple Buds with a prophyll of healthy and strong length behind the 0.3-0.5cm that grow is cut into individual plant is transferred on the strengthening seedling and rooting medium, described strawberry strengthening seedling and rooting medium comprises improvement MS+0.2mgL
-1IBA+0.02mgL
-1NAA+0.6mg indolebutyric acid+0.3gL
-1Active carbon+5.0gL
-1Agar+10gL
-1Sucrose, and the pH value is 5.8, and to keep temperature be 20-25 ℃, light application time is 15-20h, cultivated for 8 weeks after, cauline leaf is grown normal, root growth is healthy and strong, plant is long during to 3-5cm, transplants;
(2) transplant:
With the described plantlet of transplant of step (1) in culturing room, with the residual medium of clear water flush away base portion, and the maintenance indoor temperature is 20-25 ℃, behind the 33d, be transplanted in the shading screen plastic tunnel of obscurity 60-70%, under temperature is 20-25 ℃ condition, be transplanted in the dish of cave, and keep relative moisture more than 80%, behind the 8d, after new root sprouting eruption, reduce gradually irrigation times and be transplanted to flowerpot and carry out Routine Management.
Strawberry strengthening seedling and rooting cultural method of the present invention is by improveing the supplementary measures such as minimal medium and composition, adjustment condition of culture, so that the plantlet of transplant survival rate can reach more than 90%, seedling early growth is healthy and strong, can effectively solve good seed germplasm degenerate problem, simultaneously Effective Raise Yield of Strawberry and quality, strengthened its economic worth.
Below disclose the present invention with preferred embodiment, so it is not intended to limiting the invention, and all employings are equal to replaces or technical scheme that the equivalent transformation mode obtains, all drops within protection scope of the present invention.
Claims (3)
1. a strawberry strengthening seedling and rooting cultural method is characterized in that, may further comprise the steps:
(1) strengthening seedling and rooting is cultivated:
The Multiple Buds with a prophyll of healthy and strong length behind the 0.3-0.5cm that grow is cut into individual plant to be transferred on the strengthening seedling and rooting medium, keeping temperature is 20-25 ℃, light application time is 15-20h, cultivate 5-10 week, guarantee that the cauline leaf growth is normal, root growth is healthy and strong, when plant length arrives 3-5cm, transplants;
(2) transplant:
With the described plantlet of transplant of step (1) in culturing room, with the residual medium of clear water flush away base portion, and the maintenance indoor temperature is 20-25 ℃, behind the 30-35d, be transplanted in the shading screen plastic tunnel of obscurity 60-70%, under temperature is 20-25 ℃ condition, be transplanted in the dish of cave, and keep relative moisture more than 80%, behind the 5-10d, after new root sprouting eruption, reduce gradually irrigation times and be transplanted to flowerpot and carry out Routine Management.
2. a kind of strawberry strengthening seedling and rooting cultural method according to claim 1 is characterized in that, described strawberry strengthening seedling and rooting medium comprises improvement MS+0.2～0.3mgL
-1IBA+0.01～0.03mgL
-1NAA+0.5～0.8mg indolebutyric acid+0.2～0.4 gL
-1Active carbon+4.0～5.0gL
-1Agar+10gL
-1Sucrose, and the pH value is 5.2～5.8.
3. a kind of strawberry strengthening seedling and rooting cultural method according to claim 2 is characterized in that, described improvement MS comprises grand nutrition element, micronutrient element and organic reagent,
The component of described grand nutrition element and its corresponding concentration are: potassium nitrate 1800 mg/L; Ammonium sulfate 1880 mg/L; Calcium chloride dihydrate 360 mg/L; Anhydrous potassium dihydrogenphosphate 150mg/L; Disodium ethylene diamine tetraacetate 32.5 mg/L; Ferrous sulfate heptahydrate 25.3 mg/L,
The component of described micronutrient element and its corresponding concentration are: four water manganese sulphates, 18.5 mg/L; Zinc sulphate 9.5mg/L; Boric acid 7.5 mg/L; Potassium iodide 1.5mg/L; Sodium molybdate 0.5mg/L; Cobalt chloride 0.03 mg/L,
And the component of described organic reagent and its corresponding concentration are: thiamine hydrochloride 15.0 mg/L; Nicotinic acid 2.0 mg/L; Puridoxine hydrochloride 2.0mg/L; Inositol 80.0 mg/L.
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| CN201310297283.6A CN103348915B (en) | 2013-07-16 | 2013-07-16 | Method for strong seedling and rooting culture of strawberries |
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| CN201310297283.6A CN103348915B (en) | 2013-07-16 | 2013-07-16 | Method for strong seedling and rooting culture of strawberries |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104041410A (en) * | 2014-06-05 | 2014-09-17 | 卞佳林 | Strong strawberry seedling rooting culture method |
| CN106508351A (en) * | 2016-10-22 | 2017-03-22 | 侯亮 | Wild strawberry planting method |
| CN107333616A (en) * | 2017-08-07 | 2017-11-10 | 镇江万山红遍农业园 | A kind of peach is colonized culture of rootage method |
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| CN101049090A (en) * | 2007-05-22 | 2007-10-10 | 南京农业大学 | Method for carrying out taking off poison and quick breeding by using strawberry anther |
| JP2009118835A (en) * | 2007-11-13 | 2009-06-04 | Kankyo Saien:Kk | Liquid culture method in plant seedling production which raises seedling from runner |
| CN102369880A (en) * | 2010-08-09 | 2012-03-14 | 苗茹 | Strawberry detoxification and rapid propagation technology |
| CN102986534A (en) * | 2012-12-14 | 2013-03-27 | 苏州和美生物科技有限公司 | Initial culture medium special for preventing brown stain of strawberries and method for producing tissue culture strawberry seedlings by using initial culture medium |
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2013
- 2013-07-16 CN CN201310297283.6A patent/CN103348915B/en active Active
Patent Citations (4)
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|---|---|---|---|---|
| CN101049090A (en) * | 2007-05-22 | 2007-10-10 | 南京农业大学 | Method for carrying out taking off poison and quick breeding by using strawberry anther |
| JP2009118835A (en) * | 2007-11-13 | 2009-06-04 | Kankyo Saien:Kk | Liquid culture method in plant seedling production which raises seedling from runner |
| CN102369880A (en) * | 2010-08-09 | 2012-03-14 | 苗茹 | Strawberry detoxification and rapid propagation technology |
| CN102986534A (en) * | 2012-12-14 | 2013-03-27 | 苏州和美生物科技有限公司 | Initial culture medium special for preventing brown stain of strawberries and method for producing tissue culture strawberry seedlings by using initial culture medium |
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| SAJJAD MAHMOOD,ET AL.: "clonal propagation of strawberry through tissue culture", 《PAKISTAN J.AGRIC.RES》 * |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104041410A (en) * | 2014-06-05 | 2014-09-17 | 卞佳林 | Strong strawberry seedling rooting culture method |
| CN106508351A (en) * | 2016-10-22 | 2017-03-22 | 侯亮 | Wild strawberry planting method |
| CN107333616A (en) * | 2017-08-07 | 2017-11-10 | 镇江万山红遍农业园 | A kind of peach is colonized culture of rootage method |
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| CN103348915B (en) | 2014-11-05 |
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