CN102369880A - Strawberry detoxification and rapid propagation technology - Google Patents
Strawberry detoxification and rapid propagation technology Download PDFInfo
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- CN102369880A CN102369880A CN2010102533337A CN201010253333A CN102369880A CN 102369880 A CN102369880 A CN 102369880A CN 2010102533337 A CN2010102533337 A CN 2010102533337A CN 201010253333 A CN201010253333 A CN 201010253333A CN 102369880 A CN102369880 A CN 102369880A
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Abstract
The invention relates to a strawberry cultivation and breeding technology, particularly a strawberry detoxification and rapid propagation technology, belonging to the technical field of fruit processing. According to the invention, the detoxification is carried out on the strawberry by using anther culture, ultralow temperature processing and other methods, a high-efficiency strawberry detoxification breeding system is established, SMYEV virus can be effectively removed conveniently by using ultralow temperature detoxification method. The use of the ultralow temperature detoxification method has high detoxification rate, is easy and feasible and is convenient for operation.
Description
Technical field
The present invention relates to a kind of cultivated strawberry, propagation technique, be specifically related to a kind of strawberry detoxifying fast breeding technique, belong to woods fruit processing technique field.
Background technology
In recent years, it is individual less than normal that the serious problem of strawberry virus infections has become the strawberry fruit that causes production, inferior quality, fruit irregular key factor.The same with other asexually propagated plant, strawberry is easily infected virus in process of production, thereby causes the kind sexual involution of strawberry cultivars.Therefore, vegetative propagation quantity is big more, and virus disseminating is also fast more.Degradation phenomena such as be prone to behind the strawberry infective virus that leaf-shrinkage, fruit deformity, article qualitative change are bad, output decline, plant strain growth are slow.Virus is a key factor that causes that the strawberry yield and quality descends.
Summary of the invention
The objective of the invention is provides a kind of strawberry detoxifying fast breeding technique for overcoming the weak point of above-mentioned prior art, adopts methods such as anther culture and ultralow temperature processing; Strawberry is carried out detoxification treatment; Set up the breeding of method of strawberry detoxification efficiently, that utilizes that the ultralow temperature detoxicity method can be simple and effective removes SMYEV virus, and ultralow temperature detoxicity method not only virus elimination rate is very high; And simple, be convenient to operation.
The present invention realizes with following technical scheme: a kind of strawberry detoxifying fast breeding technique is characterized in that: this technology comprises anther culture detoxification, ultralow temperature processing detoxification, specifically carries out as follows:
(1) anther culture detoxification: will be taken from the healthy and strong strawberry bud in field; Developmental stage is monokaryon keep to the side the phase (transverse diameter 4-6mm); 4 ℃ of cold treatment 72h in refrigerator, then with bud respectively with the sterilization of 70% alcohol and 0.1% mercuric chloride, strip flower pesticide after the bud that disinfects blotted surperficial water with aseptic blotting paper; Be inoculated in the callus inducing medium; Forward to then in the regeneration culture medium, again the strawberry healthy seedling that induces is transferred on the subculture medium, forward the root media processing of taking root at last to;
(2) ultralow temperature is handled detoxification: utilize to combine interior target RT-PCR to carry out the detection of strawberry light yellow edge virus (SMYEV), to containing this viral plant, the allocation stem apex is done initial culture; Subculture expands after numerous 5 times; Stem apex about allocation 2mm utilizes vitrification ultra-low temperature liquid nitrogen treatment technology that SMYEV is removed, in viral subtractive process; Cultivating sucrose concentration in advance is 0.5mol/L, handles 3d; Loading is treated to 25 ℃, 1h; Vitrifying is treated to 0 ℃, 2h; Liquid nitrogen carries out 40 ℃ of water-bath 2min after handling 1h, and the survival rate of strawberry stem tip is 76%, and the virus elimination rate of light-duty yellow edge poison reaches 95%.
Advantage of the present invention is: employing should technology be carried out detoxification treatment to strawberry; Set up the breeding of method of strawberry detoxification efficiently, that utilizes that the ultralow temperature detoxicity method can be simple and effective removes SMYEV virus, and ultralow temperature detoxicity method not only virus elimination rate is very high; And simple, be convenient to operation.
Embodiment
Embodiment,
A kind of strawberry detoxifying fast breeding technique, this technology comprise anther culture detoxification, ultralow temperature processing detoxification, specifically carry out as follows:
(1) anther culture detoxification: will be taken from the healthy and strong strawberry bud in field; Developmental stage is monokaryon keep to the side the phase (transverse diameter 4-6mm); 4 ℃ of cold treatment 72h in refrigerator, then with bud respectively with the sterilization of 70% alcohol and 0.1% mercuric chloride, strip flower pesticide after the bud that disinfects blotted surperficial water with aseptic blotting paper; Be inoculated in the callus inducing medium; Forward to then in the regeneration culture medium, again the strawberry healthy seedling that induces is transferred on the subculture medium, forward the root media processing of taking root at last to;
(2) ultralow temperature is handled detoxification: utilize to combine interior target RT-PCR to carry out the detection of strawberry light yellow edge virus (SMYEV), to containing this viral plant, the allocation stem apex is done initial culture; Subculture expands after numerous 5 times; Stem apex about allocation 2mm utilizes vitrification ultra-low temperature liquid nitrogen treatment technology that SMYEV is removed, in viral subtractive process; Cultivating sucrose concentration in advance is 0.5mol/L, handles 3d; Loading is treated to 25 ℃, 1h; Vitrifying is treated to 0 ℃, 2h; Liquid nitrogen carries out 40 ℃ of water-bath 2min after handling 1h, and the survival rate of strawberry stem tip is 76%, and the virus elimination rate of light-duty yellow edge poison reaches 95%.
Claims (1)
1. strawberry detoxifying fast breeding technique is characterized in that: this technology comprises that anther culture detoxification, ultralow temperature handles detoxification, specifically carries out as follows:
(1) anther culture detoxification: will be taken from the healthy and strong strawberry bud in field; Developmental stage is monokaryon keep to the side the phase (transverse diameter 4-6mm); 4 ℃ of cold treatment 72h in refrigerator, then with bud respectively with the sterilization of 70% alcohol and 0.1% mercuric chloride, strip flower pesticide after the bud that disinfects blotted surperficial water with aseptic blotting paper; Be inoculated in the callus inducing medium; Forward to then in the regeneration culture medium, again the strawberry healthy seedling that induces is transferred on the subculture medium, forward the root media processing of taking root at last to;
(2) ultralow temperature is handled detoxification: utilize to combine interior target RT-PCR to carry out the detection of strawberry light yellow edge virus (SMYEV), to containing this viral plant, the allocation stem apex is done initial culture; Subculture expands after numerous 5 times; Stem apex about allocation 2mm utilizes vitrification ultra-low temperature liquid nitrogen treatment technology that SMYEV is removed, in viral subtractive process; Cultivating sucrose concentration in advance is 0.5mol/L, handles 3d; Loading is treated to 25 ℃, 1h; Vitrifying is treated to 0 ℃, 2h; Liquid nitrogen carries out 40 ℃ of water-bath 2min after handling 1h, and the survival rate of strawberry stem tip is 76%, and the virus elimination rate of light-duty yellow edge poison reaches 95%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102533337A CN102369880A (en) | 2010-08-09 | 2010-08-09 | Strawberry detoxification and rapid propagation technology |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102533337A CN102369880A (en) | 2010-08-09 | 2010-08-09 | Strawberry detoxification and rapid propagation technology |
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| CN102369880A true CN102369880A (en) | 2012-03-14 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2010102533337A Pending CN102369880A (en) | 2010-08-09 | 2010-08-09 | Strawberry detoxification and rapid propagation technology |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102893869A (en) * | 2012-10-22 | 2013-01-30 | 浙江省农业科学院 | Root tip detoxification and rapid propagation technology of strawberries |
| CN103348915A (en) * | 2013-07-16 | 2013-10-16 | 句容市春城镇年胜葡萄园 | Method for strong seedling and rooting culture of strawberries |
| CN104429940A (en) * | 2014-10-23 | 2015-03-25 | 绍兴文理学院 | Method for acquiring virus-free strawberry seedlings |
| CN104604685A (en) * | 2015-01-31 | 2015-05-13 | 四川农业大学 | Ultralow temperature detoxification method for strawberry stem tip |
| CN109329027A (en) * | 2018-12-03 | 2019-02-15 | 湖南省作物研究所 | A kind of efficient propagation method of sweet potato |
-
2010
- 2010-08-09 CN CN2010102533337A patent/CN102369880A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102893869A (en) * | 2012-10-22 | 2013-01-30 | 浙江省农业科学院 | Root tip detoxification and rapid propagation technology of strawberries |
| CN103348915A (en) * | 2013-07-16 | 2013-10-16 | 句容市春城镇年胜葡萄园 | Method for strong seedling and rooting culture of strawberries |
| CN104429940A (en) * | 2014-10-23 | 2015-03-25 | 绍兴文理学院 | Method for acquiring virus-free strawberry seedlings |
| CN104429940B (en) * | 2014-10-23 | 2016-06-29 | 绍兴文理学院 | A kind of method obtaining Fructus Fragariae Ananssae detoxic seedling |
| CN104604685A (en) * | 2015-01-31 | 2015-05-13 | 四川农业大学 | Ultralow temperature detoxification method for strawberry stem tip |
| CN109329027A (en) * | 2018-12-03 | 2019-02-15 | 湖南省作物研究所 | A kind of efficient propagation method of sweet potato |
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Application publication date: 20120314 |
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| WD01 | Invention patent application deemed withdrawn after publication |