CN103342748A - Special extraction device for main ingredient collagen II of natural cartilage matrix - Google Patents
Special extraction device for main ingredient collagen II of natural cartilage matrix Download PDFInfo
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- CN103342748A CN103342748A CN2013103201629A CN201310320162A CN103342748A CN 103342748 A CN103342748 A CN 103342748A CN 2013103201629 A CN2013103201629 A CN 2013103201629A CN 201310320162 A CN201310320162 A CN 201310320162A CN 103342748 A CN103342748 A CN 103342748A
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Abstract
The invention discloses a special extraction device for main ingredient collagen II of natural cartilage matrix. The special extraction device comprises an incubator, a centrifugal device arranged in the incubator, and a control system used for controlling the temperature in the incubator and the action of the centrifugal device, wherein the centrifugal device comprises a centrifugal machine, a container device installed on the centrifugal machine, a liquid adding system used for adding a reagent into the container device, a filtering and liquid discharging system used for discharging waste liquid in the container device, and a stirring system used for stirring the reagent. The special extraction device for main ingredient collagen II of natural cartilage matrix disclosed by the invention can be used for automatically completing the extraction of the main ingredient collagen II of the natural cartilage matrix, effectively saving the manpower, reducing the production cost and improving the production efficiency.
Description
Technical field
The present invention relates to a kind of professional production device of type, be specifically related to a kind of natural cartilage matrix main component-type and extract isolated plant, the type of extraction is mainly used in the preparation of Injectable tissue engineering cartilage gel matrix.
Background technology
Clinical common by wound or the joint caused position of osteopathy cartilage defect, have a strong impact on patients ' life quality, become the one of the main reasons of present limbs disability.U.S.'s sickness rate is 1.5 ‰～3 ‰, and China is about 5～6 times of the U.S., and is ascendant trend year by year.Joint cartilage belongs to hyaline cartilage, lacks neural blood vessel nutrition, and the damage back self is difficult to repair.All there is open defect in clinical existing treatment measure, and its expectant treatment and joint debridement art can only respite pain, can not stop the development of the course of disease; The bone and cartilage autotransplantation art can cause the damage for the district, and it is limited to originate, and it is damaged to be difficult to repair larger area; The allograph bone cartilage grafting exists immunological rejection and pathophorous possibility; Artificial joint replacement then the expense costliness, complication is more, revision rate is higher, particularly big to the influence of the body and mind of young patient, economical load is heavy.
The birth of organizational engineering and fast development thereof provide new technology for the Regeneration and Repair of joint cartilage.Tissue engineering bracket material is conducive to sticking, breed and even breaking up of cell for the three-dimensional rack that the cell that makes up tissue provides, for the cell growth provides suitable external environment.In organizational project, timbering material plays the effect of extracellular matrix, is bionical to the structure and function of extracellular matrix.It not only plays a supportive role, and keeps former shape in a organized way, but also plays template action, for cell provides the place of the boarding of relying, growth, differentiation and propagation, thereby guides the regeneration of damaged tissue and the structure of control regenerating tissues.
Yet, because tissue engineering bracket material is generally chosen and the bionical starting material of human body natural joint cartilage cell epimatrix main component identical materials as the preparation bionic cartilage extracellular matrix for tissue engineering, in preparation process, need through a plurality of steps and processing for a long time, and in preparation process, need long stirring, centrifugal treating, prepare a tissue engineering bracket material and will spend 20 days time usually, if all adopt manual operation, then can cause human cost to increase and produce the defectives such as efficient reduction of preparation.
Summary of the invention
In view of this, the object of the present invention is to provide a kind of natural cartilage matrix main component-type to extract isolated plant, this type extracts the preparation that isolated plant can be finished tissue engineering bone/cartilage collagen protein system automatically, can effectively save manpower, reduce cost of manufacture and enhance productivity.
For achieving the above object, the invention provides following technical scheme:
A kind of natural cartilage matrix main component-type extracts isolated plant, comprise thermostat container, be arranged on the centrifugal device in the described thermostat container and be used for control constant temperature the temperature inside the box and the Controlling System of centrifugal device action, described centrifugal device comprises whizzer, is installed in the vessel assembly on the whizzer, is used for adding in vessel assembly adding liquid system, be used for the filtration drainage system of waste liquid in the amount discharge container device and be used for stirring the stirring system of reagent of reagent;
Described whizzer comprises base and the rotating disk that is arranged on the base, is provided with in the described base for driving the rotating disk motor rotating;
Described vessel assembly comprises the container holder that is fixedly mounted on the described rotating disk, vertically be provided with the container that two-stage at least is used for holding reagent on the described container holder, and in the adjacent two-stage container, superposed container bottom is provided with the passage that communicates with the container that is positioned at the bottom;
Described filtration drainage system comprises that waste liquid tank and being arranged on is positioned at the described container bottom of below and is used for discharging the apocenosis passage of waste liquid, be equipped with semi-permeable membranes and control valve on described passage and the apocenosis passage, the corresponding sump pit that is provided with described apocenosis passage is provided with the waste collection passage between described sump pit and the described waste liquid tank on the described base;
Described stirring system comprises the seat of honour that is arranged on described vessel assembly top, and the bottom surface at the described seat of honour is provided with and the described container agitator arm group of corresponding setting one by one, is provided with the drive unit that moves up and down for the described seat of honour of driving between the described seat of honour and the base;
The described liquid system that adds comprises and is installed in the reagent trough that described seat of honour end face is used for holding reagent or reagent container is installed, the corresponding liquid feeding passage that is provided with one by one between described reagent trough and the described container, and described liquid feeding passage is provided with control valve;
Described Controlling System comprises controller, all link to each other with described controller and be used for to measure described constant temperature the temperature inside the box temperature sensor, be used for timing timing register, be used for the motor-drive circuit of the described motor action of control and be used for the control module of the described drive unit lifting action of control, be provided with temperature control output system in the described thermostat container, described controller links to each other with temperature control output system with described control valve respectively.
Further, described drive unit comprises the tail rod that is arranged between the described seat of honour and the base and the cylinder that is used for driving the tail rod stretching motion, and the gas circuit of described cylinder is provided with magnetic valve, and described control module links to each other with described magnetic valve.
Further, be arranged on the bulge that container on the described container holder all is coaxial setting, and in the adjacent two-stage container, be positioned at the cross-sectional area of container of bottom greater than the cross-sectional area of superposed container.
Further, described agitator arm group correspondence is arranged between the described container of adjacent two-stage, and is arranged on the vessel port top of this container with the agitator arm group of the corresponding setting of described container that is positioned at the top.
Further, described agitator arm group comprises at least one agitator arm.
Further, described liquid feeding passage comprise with described reagent trough one by one corresponding be arranged in the described seat of honour liquid feeding passage I be arranged on described agitator arm in the liquid feeding passage II that links to each other with described liquid feeding passage I respectively.
Further, described control valve is arranged on the described liquid feeding passage I.
Further, described container holder is provided with the PH sensor that also is used for measuring pH value in the described container be used to being placed on, and described PH sensor links to each other with described controller.
Further, described agitator arm is provided with agitating vane.
Beneficial effect of the present invention is:
Natural cartilage matrix main component of the present invention-type extracts isolated plant, by centrifugal device is set, under the effect of whizzer, can drive the vessel assembly high speed rotating, and the reagent that is contained in the vessel assembly is carried out centrifugal treating; By multistage container is set, and passage is set between adjacent container in vessel assembly, in conjunction with the filtrate drainage system, under the effect of semi-permeable membranes, can separation of supernatant and throw out, and select to keep supernatant liquor or throw out continues preparation; By stirring system is set, when needs stir, the agitator arm group is extend in the corresponding container, drive vessel assembly with respect to the agitator arm low speed rotation by whizzer, can realize the agitating function of solution; Add liquid system by setting, can in preparation process, in the container of correspondence, add reagent;
By Controlling System is set, controller sends steering order by the temperature signal of temperature sensor to the temperature control output system of thermostat container, makes the temperature in the thermostat container can satisfy the preparation requirement all the time; Can control motor startup-shutdown, rotating speed and rotational time etc. by motor-drive circuit, and by the up-and-down movement of control module accessory drive, stirred solution or centrifugal solution when needed; By the control to control valve, can in container, inject required reagent when needed;
Concrete, it is as follows to adopt natural cartilage matrix main component of the present invention-type extraction isolated plant to prepare the method for type:
1) under room temperature, aseptic condition, from the fresh pig femur, cut knee cartilage with scalpel, place physiological saline temporary;
2) the cartilage thin slice of washes clean is placed carry out freeze-drying in the low-temperature freeze drying machine, pulverize with Lowtemperaturepulverizer after removing moisture fully, and take by weighing chondroconia weight;
3) cartilage after will pulverizing places the superiors' container, and to inject volume in the container be 75% medical alcohol of 5 times of cartilage volumes, 24h sterilizes in 4 ℃ and gnotobasis, after sterilization is finished, utilize whizzer with the rotating speed centrifugal solution 20min of 8000rpm, open control valve after centrifugal, utilize semi-permeable membranes to filter out supernatant liquor, the throw out of reservation fully washs with distilled water;
4) injecting volume in the superiors' container is the 3rd) the 4mol/L guanidine hydrochloride solution of 10 times of volumes of step gained throw out, with throw out and this guanidine hydrochloride solution suspendible, then agitator arm is stretched in the container, utilize whizzer to drive the vessel assembly low speed rotation, behind the stirred solution 24h agitator arm is withdrawed from outside the container, utilize whizzer with the rotating speed centrifugal solution 20min of 8000rpm, open control valve after centrifugal, utilize semi-permeable membranes to filter out supernatant liquor, the throw out of reservation fully washs with 0.05mol/L Tris-HCl damping fluid and 0.5mol/L Glacial acetic acid;
5) injecting volume in the first step container is the 4th) the 1g/L stomach en--Glacial acetic acid Digestive system of 5 times of volumes of step gained throw out, in the container of the second stage, inject an amount of scavenging solution, then agitator arm is stretched in the container, utilize whizzer to drive the vessel assembly low speed rotation, behind the stirred solution 48h agitator arm is withdrawed from outside the container, utilize whizzer with the rotating speed centrifugal solution 20min of 8000rpm, centrifugal back is got rid of the scavenging solution in the container of the second stage earlier, and the recycling semi-permeable membranes is collected supernatant liquor in the partial container; After can continuing to add Digestive system in the throw out and digesting 24h, and supernatant liquor is collected in the container of the second stage;
6) in the container of the second stage, inject the rapid regulator solution of 2mol/L NaOH to pH7.5, it is 3mol/L that adding NaCl makes its ultimate density, under 4 ℃ and gnotobasis, saltout and spend the night, utilize whizzer with the rotating speed centrifugal solution 20min of 8000rpm, utilize semi-permeable membranes to filter out supernatant liquor, the throw out of reservation;
7) continue to inject tri-distilled water in the container of the second stage and dialyse, after dialysis was finished, the rotating speed centrifugal solution 20min with 8000rpm utilized semi-permeable membranes to filter out supernatant liquor, and the throw out of reservation namely obtains collagen protein;
In summary, natural cartilage matrix main component of the present invention-type extracts the preparation that isolated plant can be finished tissue engineering bone/cartilage collagen protein system automatically, can effectively save manpower, reduces cost of manufacture and enhances productivity.
Description of drawings
In order to make purpose of the present invention, technical scheme and beneficial effect clearer, the invention provides following accompanying drawing and describe:
Fig. 1 is the structural representation that natural cartilage matrix main component of the present invention-type extracts isolated plant embodiment;
Fig. 2 is the A detail drawing of Fig. 1;
Fig. 3 is the control principle block diagram;
Fig. 4 is the sectional view of agitator arm.
Embodiment
Below in conjunction with accompanying drawing, the preferred embodiments of the present invention are described in detail.
As shown in Figure 1, natural cartilage matrix main component of the present invention-type extracts the structural representation of isolated plant embodiment.The natural cartilage matrix main component of present embodiment-type extracts isolated plant, the Controlling System that comprises thermostat container 1, is arranged on the centrifugal device in the thermostat container 1 and moves for temperature and centrifugal device in the control thermostat container 1, centrifugal device comprises whizzer, is installed in the vessel assembly on the whizzer, is used for adding in vessel assembly adding liquid system, be used for the filtration drainage system of waste liquid in the amount discharge container device and be used for stirring the stirring system of reagent of reagent.
Whizzer comprises base 2 and the rotating disk 3 that is arranged on the base, is provided with in the base 2 for driving rotating disk motor rotating 4, is used for driving the rotation of vessel assembly high speed centrifugation or stirring at low speed and rotates.
Vessel assembly comprises the container holder 5 that is fixedly mounted on the rotating disk 3, vertically be provided with the container 6 that two-stage at least is used for holding reagent on the container holder 5, and in the adjacent two-stage container 6, superposed container 6 bottoms are provided with the passage 7 that communicates with the container that is positioned at the bottom.
Filtering drainage system comprises waste liquid tank 8 and is arranged on container 6 bottoms that are positioned at below and the apocenosis passage 9 that is used for discharging waste liquid, be equipped with semi-permeable membranes 10 and control valve 11 on passage 7 and the apocenosis passage 9, on the base 2 with the apocenosis passage 9 corresponding sump pits 12 that are provided with, be provided with waste collection passage 13 between sump pit 12 and the waste liquid tank 8, by at passage 7 and apocenosis passage 9 semi-permeable membranes 10 and control valve 11 being set, in preparation process, can select to keep throw out or cleaner liquid.
Stirring system comprises the seat of honour 14 that is arranged on the vessel assembly top, the bottom surface at the seat of honour 14 is provided with and the container 6 agitator arm group of corresponding setting one by one, be provided with between the seat of honour 14 and the base 2 for driving the drive unit that the seat of honour 14 moves up and down, by moving up and down of the seat of honour 14, the agitator arm group can be stretched into or withdraws from container 6.
Adding liquid system comprises and is installed in the reagent trough 15 that the seat of honour 14 end faces are used for holding reagent or reagent container is installed, the corresponding liquid feeding passage 16 that is provided with one by one between reagent trough 15 and the container 6, liquid feeding passage 16 is provided with control valve 17, by at liquid feeding passage 16 control valve 17 being set, by the switching of control control valve 17, can control to kind and the opportunitys of container 6 interior interpolation reagent.
Controlling System comprises controller 18, all link to each other with controller 18 and be used for to measure temperature in the thermostat container 1 temperature sensor 19, be used for timing timing register 20, be used for the motor-drive circuit 21 of control motor 4 actions and be used for the control module 22 of accessory drive lifting action, be provided with temperature control output system 23 in the thermostat container 1, controller 18 links to each other with temperature control output system 23 with control valve 17 respectively.
Preferably, the drive unit of present embodiment comprises the tail rod 24 that is arranged between the seat of honour 14 and the base 2 and the cylinder that is used for driving tail rod 24 stretching motions, the gas circuit of cylinder is provided with magnetic valve 25, and control module 22 links to each other with magnetic valve 25, can drive the seat of honour 14 by cylinder and move up and down.
Further, be arranged on the bulge that container 6 on the container holder 5 all is coaxial setting, and in the adjacent two-stage container 6, be positioned at the cross-sectional area of container 6 of bottom greater than the cross-sectional area of superposed container 6, adopt the vessel assembly of this structure, can make things convenient for the stirring of each container 6.Preferably, agitator arm group correspondence is arranged between the adjacent two-stage container 6, and is arranged on the vessel port top of this container 6 with the agitator arm group of the container 6 corresponding settings that are positioned at the top, can easily the agitator arm group be stretched into or withdraw from container 6.Preferably, the agitator arm group comprises at least one agitator arm 26, the container holder 5 of present embodiment is provided with two-stage container 5, the agitator arm group corresponding with the first step container of the top comprises an agitator arm 26, the agitator arm group corresponding with second stage container comprises four agitator arms 26 that annular is uniform, agitator arm 26 is provided with agitating vane 26a, can strengthen stirring capacity.
Further, liquid feeding passage 16 comprise with reagent trough 15 one by one corresponding be arranged in the seat of honour liquid feeding passage I be arranged on agitator arm 26 in the liquid feeding passage II16a that links to each other with liquid feeding passage I respectively, by liquid feeding passage II16a is arranged in the agitator arm 26, can simplified construction, and utilize agitator arm 26 to add reagent to container.Preferably, control valve 10 is arranged on the liquid feeding passage I.
Further, container holder 5 is provided with the PH sensor 27 that also is used for measuring pH value in the container 6 be used to being placed on, and PH sensor 27 links to each other with controller 18.
The natural cartilage matrix main component of present embodiment-type extracts isolated plant, by centrifugal device is set, under the effect of whizzer, can drive the vessel assembly high speed rotating, and the reagent that is contained in the vessel assembly is carried out centrifugal treating; By multistage container 6 is set, and between adjacent container 6, passage is set in vessel assembly, in conjunction with the filtrate drainage system, under the effect of semi-permeable membranes 10, can separation of supernatant and throw out, and select to keep supernatant liquor or throw out continues preparation; By stirring system is set, when needs stir, the agitator arm group is extend in the corresponding container, drive vessel assembly with respect to agitator arm 26 low speed rotation by whizzer, can realize the agitating function of solution; Add liquid system by setting, can in preparation process, in the container of correspondence, add reagent;
By Controlling System is set, controller 18 sends steering order by the temperature signal of temperature sensor 19 to the temperature control output system 23 of thermostat container 1, makes the temperature in the thermostat container 1 can satisfy the preparation requirement all the time; Can control motor 4 startup-shutdowns, rotating speed and rotational time etc. by motor-drive circuit 21, and by control module 22 accessory drive up-and-down movement, stirred solution or centrifugal solution when needed; By the control to control valve 17, can in container 6, inject required reagent when needed.
Concrete, the method that employing present embodiment natural cartilage matrix main component-type extraction isolated plant prepares type is as follows:
1) under room temperature, aseptic condition, from the fresh pig femur, cut knee cartilage with scalpel, place physiological saline temporary;
2) the cartilage thin slice of washes clean is placed carry out freeze-drying in the low-temperature freeze drying machine, pulverize with Lowtemperaturepulverizer after removing moisture fully, and take by weighing chondroconia weight;
3) cartilage after will pulverizing places the superiors' container, and to inject volumes in the container 6 be 75% medical alcohol of 5 times of cartilage volumes, 24h sterilizes in 4 ℃ and gnotobasis, after sterilization is finished, utilize whizzer with the rotating speed centrifugal solution 20min of 8000rpm, open control valve 11 after centrifugal, utilize semi-permeable membranes 10 to filter out supernatant liquor, the throw out of reservation fully washs with distilled water;
4) injecting volume in the superiors' container is the 3rd) the 4mol/L guanidine hydrochloride solution of 10 times of volumes of step gained throw out, with throw out and this guanidine hydrochloride solution suspendible, then agitator arm 26 is stretched in the container 6, utilize whizzer to drive the vessel assembly low speed rotation, behind the stirred solution 24h agitator arm is withdrawed from outside the container, utilize whizzer with the rotating speed centrifugal solution 20min of 8000rpm, open control valve after centrifugal, utilize semi-permeable membranes 10 to filter out supernatant liquor, the throw out of reservation fully washs with 0.05mol/L Tris-HCl damping fluid and 0.5mol/L Glacial acetic acid;
5) injecting volume in the first step container 6 is the 4th) the 1g/L stomach en--Glacial acetic acid Digestive system of 5 times of volumes of step gained throw out, in the container of the second stage, inject an amount of scavenging solution, then agitator arm is stretched in the container, utilize whizzer to drive the vessel assembly low speed rotation, behind the stirred solution 48h agitator arm is withdrawed from outside the container, utilize whizzer with the rotating speed centrifugal solution 20min of 8000rpm, centrifugal back is got rid of the scavenging solution in the container of the second stage earlier, and the recycling semi-permeable membranes is collected supernatant liquor in the partial container; After can continuing to add Digestive system in the throw out and digesting 24h, and supernatant liquor is collected in the container of the second stage;
6) in the container of the second stage, inject the rapid regulator solution of 2mol/L NaOH to pH7.5, it is 3mol/L that adding NaCl makes its ultimate density, under 4 ℃ and gnotobasis, saltout and spend the night, utilize whizzer with the rotating speed centrifugal solution 20min of 8000rpm, utilize semi-permeable membranes to filter out supernatant liquor, the throw out of reservation;
7) continue to inject tri-distilled water in the container of the second stage and dialyse, after dialysis was finished, the rotating speed centrifugal solution 20min with 8000rpm utilized semi-permeable membranes to filter out supernatant liquor, and the throw out of reservation namely obtains collagen protein;
In summary, natural cartilage matrix main component of the present invention-type extracts the preparation that isolated plant can be finished tissue engineering bone/cartilage collagen protein system automatically, can effectively save manpower, reduces cost of manufacture and enhances productivity.
Explanation is at last, above preferred embodiment is only unrestricted in order to technical scheme of the present invention to be described, although the present invention is described in detail by above preferred embodiment, but those skilled in the art are to be understood that, can make various changes to it in the form and details, and not depart from claims of the present invention institute restricted portion.
Claims (9)
1. natural cartilage matrix main component-type extracts isolated plant, it is characterized in that: comprise thermostat container, be arranged on the centrifugal device in the described thermostat container and be used for control constant temperature the temperature inside the box and the Controlling System of centrifugal device action, described centrifugal device comprises whizzer, is installed in the vessel assembly on the whizzer, is used for adding in vessel assembly adding liquid system, be used for the filtration drainage system of waste liquid in the amount discharge container device and be used for stirring the stirring system of reagent of reagent;
Described whizzer comprises base and the rotating disk that is arranged on the base, is provided with in the described base for driving the rotating disk motor rotating;
Described vessel assembly comprises the container holder that is fixedly mounted on the described rotating disk, vertically be provided with the container that two-stage at least is used for holding reagent on the described container holder, and in the adjacent two-stage container, superposed container bottom is provided with the passage that communicates with the container that is positioned at the bottom;
Described filtration drainage system comprises that waste liquid tank and being arranged on is positioned at the described container bottom of below and is used for discharging the apocenosis passage of waste liquid, be equipped with semi-permeable membranes and control valve on described passage and the apocenosis passage, the corresponding sump pit that is provided with described apocenosis passage is provided with the waste collection passage between described sump pit and the described waste liquid tank on the described base;
Described stirring system comprises the seat of honour that is arranged on described vessel assembly top, and the bottom surface at the described seat of honour is provided with and the described container agitator arm group of corresponding setting one by one, is provided with the drive unit that moves up and down for the described seat of honour of driving between the described seat of honour and the base;
The described liquid system that adds comprises and is installed in the reagent trough that described seat of honour end face is used for holding reagent or reagent container is installed, the corresponding liquid feeding passage that is provided with one by one between described reagent trough and the described container, and described liquid feeding passage is provided with control valve;
Described Controlling System comprises controller, all link to each other with described controller and be used for to measure described constant temperature the temperature inside the box temperature sensor, be used for timing timing register, be used for the motor-drive circuit of the described motor action of control and be used for the control module of the described drive unit lifting action of control, be provided with temperature control output system in the described thermostat container, described controller links to each other with temperature control output system with described control valve respectively.
2. natural cartilage matrix main component according to claim 1-type extracts isolated plant, it is characterized in that: described drive unit comprises the tail rod that is arranged between the described seat of honour and the base and the cylinder that is used for driving the tail rod stretching motion, the gas circuit of described cylinder is provided with magnetic valve, and described control module links to each other with described magnetic valve.
3. the extraction element of tissue engineering bone/cartilage main matrix composition-type according to claim 1 and 2, it is characterized in that: be arranged on the bulge that container on the described container holder all is coaxial setting, and in the adjacent two-stage container, be positioned at the cross-sectional area of container of bottom greater than the cross-sectional area of superposed container.
4. the extraction element of tissue engineering bone/cartilage main matrix composition-type according to claim 3, it is characterized in that: described agitator arm group correspondence is arranged between the described container of adjacent two-stage, and is arranged on the vessel port top of this container with the agitator arm group of the corresponding setting of described container that is positioned at the top.
5. natural cartilage matrix main component according to claim 4-type extracts isolated plant, and it is characterized in that: described agitator arm group comprises at least one agitator arm.
6. natural cartilage matrix main component according to claim 5-type is extracted isolated plant, it is characterized in that: described liquid feeding passage comprise with described reagent trough one by one corresponding be arranged in the described seat of honour liquid feeding passage I be arranged on described agitator arm in the liquid feeding passage II that links to each other with described liquid feeding passage I respectively.
7. natural cartilage matrix main component according to claim 6-type extracts isolated plant, and it is characterized in that: described control valve is arranged on the described liquid feeding passage I.
8. natural cartilage matrix main component according to claim 1-type extracts isolated plant, it is characterized in that: described container holder is provided with the PH sensor that also is used for measuring pH value in the described container be used to being placed on, and described PH sensor links to each other with described controller.
9. natural cartilage matrix main component according to claim 5-type extracts isolated plant, and it is characterized in that: described agitator arm is provided with agitating vane.
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| CN106366181A (en) * | 2016-09-14 | 2017-02-01 | 成都百奥诺维生物科技有限公司 | High-linearity temperature monitoring system for hydrolysis extraction of fishskin collagen |
| CN106441614A (en) * | 2016-09-14 | 2017-02-22 | 成都百奥诺维生物科技有限公司 | High-precision temperature monitoring system for hydrolysis-based fish skin collagen extraction |
| CN106432476A (en) * | 2016-09-14 | 2017-02-22 | 成都百奥诺维生物科技有限公司 | Signal compensation type temperature monitoring system for extracting fish skin collagen through hydrolysis method |
| CN106441615A (en) * | 2016-09-14 | 2017-02-22 | 成都百奥诺维生物科技有限公司 | Temperature monitoring system for fish-skin collagen extraction through hydrolysis based on bias circuit |
| CN106432477A (en) * | 2016-09-14 | 2017-02-22 | 成都百奥诺维生物科技有限公司 | Temperature monitoring system for fish skin collagen extraction using hot water hydrolysis |
| CN114106147A (en) * | 2020-11-19 | 2022-03-01 | 烟台朗迪生物科技有限公司 | Fish glue protein extraction device and extraction method thereof |
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