CN208166992U - A kind of multi-functional placenta tissue processing unit - Google Patents
A kind of multi-functional placenta tissue processing unit Download PDFInfo
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- CN208166992U CN208166992U CN201820486184.0U CN201820486184U CN208166992U CN 208166992 U CN208166992 U CN 208166992U CN 201820486184 U CN201820486184 U CN 201820486184U CN 208166992 U CN208166992 U CN 208166992U
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Abstract
A kind of multi-functional placenta tissue processing unit, it is related to field of biotechnology, and in particular to a kind of processing unit of placenta tissue.The purpose of this utility model, which is that placenta tissue open treated is easy when solving conventional placenta cells cleaning, separation, to be polluted, and artificial time-consuming in treatment process, processing is incomplete, tissue space cell obtains few problem.Multi-functional placenta tissue processing unit includes that multifunctional motor-driven bracket, placenta processing unit, cleaning solution receiver, placenta shred pedestal and hematopoietic cell/tissue collector, when cleaning placenta, placenta processing unit is connect with cleaning solution receiver, is placed on multifunctional motor-driven bracket；When broken placenta, placenta processing unit shreds pedestal with placenta and connect, and is placed on multifunctional motor-driven bracket；When collection, placenta processing unit is connect with hematopoietic cell/tissue collector, is placed on multifunctional motor-driven bracket.The utility model is mainly used for obtaining placental hematopoietic stem cell/precursor/mescenchymal stem cell pretreatment.
Description
Technical field
The utility model relates to field of biotechnology, and in particular to a kind of processing unit of placenta tissue.
Background technique
It during gradually developing into an individual is obtained by placenta after the endometrium of fertilization egg implantation and parent
Nutrition and metabolism waste.Placenta tissue is an accurate structure, is during mammalian growth between parent and baby
Important protective effect and Exchange of material and energy place.One mature diameter of placenta about 160-200mm, thickness about 20-
450-650 grams of 25mm, weight, structure include amnion, chorion frondosum, decidua basalis three parts.In chorion, have a large amount of
The protrusion being made of fetus vein and artery villus leaflet so that its large specific surface area increases to greatly about 14 square metres, greatly
The mass exchange area between fetus and parent is increased greatly, also therefore assigns placenta multiple functions, including：1. natural barrier is made
With.Filtering of the extraneous substance by placenta tissue, buffer action, prevent microorganism and toxin etc. to be directly entered in infants.
2. nutrition function of exchange.The nutrition such as sugar, fat, moisture, minerals, vitamin, antibody, hormone, the amino acid of parent are logical
It crosses and actively or passively transports, send the capillary into placenta to take nutrition to villus small interlobar fissure, and by villus leaflet
Fetal capillaries are transported after absorbing to fetus.And the urea of fetus, uric acid, bilirubin, electrolyte, HCG,
Progesterone, moisture etc. are then redirected back into maternal blood, to realize the excretion of fetus bodily waste.3. gas exchanges
Place.By placenta, oxygen is conveyed to fetus by parent, and carbon dioxide is excluded fetus.4. hormone adjustment effect.Placenta
Hormone can play the role of endocrine metabolic diseases, and at the 2nd week of the pregnancy period, HCG began to secrete in the second week of gestation, by the 8th week
Reach secretion peak；HCS starts to express in the second month of gestation, peaks at 8th month；Placenta was at pregnant the 12nd week
Start just secretion estrogen and progesterone, estrogen promotes the development of myometrium and mammary gland；Progesterone then can promote in uterine mucosa
Body of gland growth, reduces the excitability of gravid uterus, reduces early stage uterine contraction, adjusts the effects of immune.5. hematopoiesis function.Fetus liver
Transfer of the hematopoiesis to marrow hemopoiesis is to have completed in 34 week early period, and contain the CD34 sun higher than arterial blood in umbilical vein blood
Property, the primitive hematopoietic stem cell and precursor that CD38 is negative, Colony forming is very capable, thus prove the part hematopoiesis of fetus
Function is from placenta.Histological research shows in placenta containing a large amount of stem cell, including candidate stem cell, hemopoietic precursors
Cell, mescenchymal stem cell etc. have to blood cell, skeletonization, at differentiation capabilities such as cartilage and fat, therefore placenta tissue
Organization material as important regenerative medicine field.
Candidate stem cell rich content in placenta is 3-5 times in Cord blood, therefore can be used as blood disease and exempt from
Predominant haematological source of human stem cell in epidemic disease disease treatment.After placenta tissue is separated after shredding, is cultivated, being expanded, acquisition
Its research application field of mescenchymal stem cell has included a variety of in tissue damage reparation, such as cirrhosis, cartilage damage, nerve
Damage, bone injury, tendon ligament injury, skin injury etc. are the important source of human stem cell of body Regeneration and Repair.
Stem cell needs to carry out various processing, including flushing, disinfection, group to placenta tissue from obtaining in placenta tissue
Knit cut small, candidate stem cell collect, mescenchymal stem cell culture.Traditional method is to use open-sky technique method,
Placenta tissue is put in a sterile plate, in safety cabinet, is successively impregnated using aseptic water washing, removal, 75% alcohol
Disinfection move back remove, sterile water rinses again, craft scissors organize to shred, sterile water immersion separate blood cells etc. are pretreated
Journey, each process are open processes, and microbial contamination risk is very big.This process needs artificial time-consuming high simultaneously, used
Instrument is also required to clean repeatedly, sterilize, therefore at high cost.
Utility model content
The purpose of this utility model is that placenta tissue open treated is easy dirt when solving conventional placenta cells cleaning, separation
It contaminates, artificial time-consuming in treatment process, processing is incomplete, tissue space cell obtains few problem, and provides a kind of multi-functional tire
Disk tissue treatment apparatus.
A kind of multi-functional placenta tissue processing unit, it includes that multifunctional motor-driven bracket, placenta processing unit, cleaning solution connect
Receipts device, placenta shred pedestal and hematopoietic cell/tissue collector,
The multifunctional motor-driven bracket includes the first belt, the electromagnetic mixer with platform, the second belt and electronic control
Device processed, the electromagnetic mixer with platform are arranged on electric controller, and the first belt and the second belt are arranged in Electronic control
Device two sides, are respectively provided with curved portion on the first belt and the second belt, and the first belt and the second belt synchronize axial counterclockwise turn
It is dynamic, but the first belt and the second belt are moved up and down in reversed；
The placenta processing unit includes closing silicon rubber cup, connecting tube, processor lid, processor cylinder and two baffles,
Connecting tube setting is in processor tops, the port setting closing silicon rubber cup of connecting tube, the lower end of processor lid and processor cylinder
Body upper end is together by a threaded connection, and two baffles are oppositely arranged on processor cylinder inboard wall；
The cleaning solution receiver include taper treatment fluid receiver, 200 microns slip through the net, cleaning solution diversion pipe, cleaning solution
Emulsion tube and cleaning solution flow-stop valve are slipped through the net for 200 microns and are arranged in taper treatment fluid receiver, at taper treatment fluid receiver bottom
End setting cleaning solution diversion pipe, cleaning solution diversion pipe end seal connect cleaning solution emulsion tube, are arranged on cleaning solution emulsion tube
Cleaning solution flow-stop valve；
It includes pedestal and magnetic force shear knife, pedestal upper end opening, lower end closed, the bottom in pedestal that the placenta, which shreds pedestal,
Magnetic force shear knife is set in portion；
Hematopoietic cell/the tissue collector include cone-shaped collection liquid receiver, 70 microns slip through the net, collection liquid diversion pipe,
Collection liquid emulsion tube and collection liquid flow-stop valve；It slips through the net for 70 microns and is arranged in cone-shaped collection liquid receiver, connect in cone-shaped collection liquid
It receives device bottom end and collection liquid diversion pipe is set, collection liquid diversion pipe end seal connects collection liquid emulsion tube, in collection liquid emulsion tube
Upper setting collection liquid flow-stop valve；
When multi-functional placenta tissue processing unit for clean placenta when, the processor cylinder lower end of placenta processing unit with
The taper treatment fluid receiver threaded upper ends of cleaning solution receiver link together, by connecting tube in the form of processor cylinder traverse
It is respectively placed in cleaning solution diversion pipe on the first belt of multifunctional motor-driven bracket and the curved portion of the second belt；
When multi-functional placenta tissue processing unit for be crushed placenta when, the processor cylinder lower end of placenta processing unit with
The pedestal threaded upper ends that placenta shreds pedestal link together, and are set in the form of pedestal and the electromagnetic mixer face contact with platform
In on multifunctional motor-driven bracket；
When multi-functional placenta tissue processing unit is used for placenta hematopoietic cell/tissue collecting, the place of placenta processing unit
Reason device cylinder lower end links together with hematopoietic cell/tissue collector cone-shaped collection liquid receiver threaded upper ends, with processing
Connecting tube and collection liquid diversion pipe are respectively placed in the first belt and the second skin of multifunctional motor-driven bracket by device cylinder traverse form
In the curved portion of band.
The utility model advantage：One, a kind of multi-functional placenta tissue processing unit of the utility model is that collection placenta tissue is clear
It washes, sterilize, shearing, candidate stem cell separation, placenta tissue block separate the multifunctional processor of the whole body.This device can be made nothing
The closed processing mode of bacterium.The processes such as cleaning, disinfection, the shearing of placenta treatment process, traditional method are all in open disk
It is carried out in son, includes the addition of multi-step, different reagents in these steps, the removal of reagent is easy in open environment
Because the problems such as operation of personnel or control of environment, brings pollution into, to the nothing of later period candidate stem cell, mescenchymal stem cell
Bacterium separation and culture threaten.Two, a kind of multi-functional placenta tissue processing unit of the utility model is that the placenta of automation is cut
Butt formula.Traditional placental hematopoietic stem cell separate mode includes that venous perfusion or manual placenta tissue shred, crush two kinds
Mode.The candidate stem cell or precursor that reperfusion mode obtains are less, therefore most of techniques can take latter manual
Shredding or crush mode, artificial caused by this mode time-consuming, and tissue block size is uneven and is unable to reach expected scale,
A large amount of manual works such as also someone to be operated simultaneously the cleaning of pollution risk and finishing operations platform, cutting implement cleaning.Using
Cut mode is automated, these disadvantages can be fully solved.Three, a kind of multi-functional placenta tissue processing unit of the utility model is adopted
With axial rotation and the asynchronous electronic hybrid mode stirred up and down, is conducive to cleaning solution and sample, thimerosal and sample, collects
Liquid is contacted with the repetition of sample, realizes that function is recycled in efficient cleaning and cell.Four, a kind of multi-functional placenta group of the utility model
Processing unit is knitted by candidate stem cell and tissue stem cell while being separated.This method can disposably obtain thin rich in Hematopoietic Stem
Born of the same parents/precursor single cell suspension, at the same isolate be used directly for adherent method culture rich in mescenchymal stem cell
Tissue block, it is easy to operate.Five, a kind of multi-functional placenta tissue processing unit structure of the utility model is simple, cheap, uses
It is easy to clean afterwards, inside there is baffle that the repetition contact of tissue and liquid, and processor cylinder, taper treatment fluid receiver may be implemented
Be translucent epoxy material with cone-shaped collection liquid receiver, not only facilitate observation container in placenta cleaning and sterilizing process and
Degree is shredded, microbial contamination can be avoided by high pressure sterilization under the conditions of 121 DEG C before reuse.
The multi-functional placenta tissue processing unit of the utility model is for obtaining placental hematopoietic stem cell/precursor/fill
The pretreatment of matter stem cell.
Detailed description of the invention
Fig. 1 is the multifunctional motor-driven supporting structure schematic diagram of the multi-functional placenta tissue processing unit of the utility model, 1 in figure
Indicate the first belt, 2 indicate the electromagnetic mixer for having platform, and 3 indicate the second belt, and 4 indicate electric controller；
Fig. 2 is the placenta processing device structure diagram of the multi-functional placenta tissue processing unit of the utility model, 5 tables in figure
Show closing silicon rubber cup, 6 indicate connecting tube, and 7 indicate processor lid, and 8 list processing device cylinders, 9 indicate baffle；
Fig. 3 is the cleaning solution receiver architecture schematic diagram of the multi-functional placenta tissue processing unit of the utility model, 10 in figure
Indicate taper treatment fluid receiver, 11, which indicate 200 microns, slips through the net, 12 expression cleaning solution diversion pipes, cleaning solution emulsion tube at 13 tables,
14 indicate cleaning solution flow-stop valve；
Fig. 4 is placenta processing unit and cleaning solution receiver corporation structural schematic diagram when cleaning placenta, 5 expression closings in figure
Silicon rubber cup, 6 indicate connecting tube, and 7 indicate processor lid, and 8 list processing device cylinders, 9 indicate baffle, and 10 indicate that taper treatment fluid receives
Device, 11, which indicate 200 microns, slips through the net, and 12 indicate cleaning solution diversion pipe, and cleaning solution emulsion tube at 13 tables, 14 indicate cleaning solution fluid stopping
Valve；
Fig. 5 is that the placenta of the multi-functional placenta tissue processing unit of the utility model shreds schematic diagram of base structure, 15 in figure
Indicate pedestal, 16 indicate magnetic force shear knife；
Fig. 6 is hematopoietic cell/tissue collector structural schematic diagram of the multi-functional placenta tissue processing unit of the utility model,
17 indicate cone-shaped collection liquid receiver in figure, and 18, which indicate 70 microns, slips through the net, and 19 indicate collection liquid diversion pipe, collection liquid cream at 20 tables
Sebific duct, 21 indicate collection liquid flow-stop valve.
Specific embodiment
Specific embodiment one：Present embodiment is a kind of multi-functional placenta tissue processing unit, it includes multi-functional electricity
Dynamic bracket, placenta processing unit, cleaning solution receiver, placenta shred pedestal and hematopoietic cell/tissue collector,
The multifunctional motor-driven bracket includes the first belt 1, the electromagnetic mixer 2 with platform, the second belt 3 and electronic
Controller 4, the electromagnetic mixer 2 with platform are arranged on electric controller 4, and the first belt 1 and the second belt 3 are arranged in electricity
4 two sides of movement controller, are respectively provided with curved portion on the first belt 1 and the second belt 3, and the first belt 1 and the second belt 3 are synchronous inverse
Hour hands axially rotate, but the first belt 1 and the second belt 3 are moved up and down in reversed；
The placenta processing unit includes closing silicon rubber cup 5, connecting tube 6, processor lid 7, processor cylinder 8 and two gears
Plate 9, the setting of connecting tube 6 is at the top of processor lid 7, the port setting closing silicon rubber cup 5 of connecting tube 6, the lower end of processor lid 7 with
8 upper end of processor cylinder is together by a threaded connection, and two baffles 9 are oppositely arranged on 8 inner wall of processor cylinder；
The cleaning solution receiver include 10,200 microns of taper treatment fluid receiver 11, cleaning solution diversion pipes 12 of slipping through the net,
Cleaning solution emulsion tube 13 and 14,200 microns of cleaning solution flow-stop valve slip through the net 11 settings in taper treatment fluid receiver 10, in taper
Cleaning solution diversion pipe 12 is arranged in 10 bottom end for the treatment of fluid receiver, and 12 end seal of cleaning solution diversion pipe connects cleaning solution emulsion tube
13, cleaning solution flow-stop valve 14 is set on cleaning solution emulsion tube 13；
It includes pedestal 15 and magnetic force shear knife 16,15 upper end opening of pedestal, lower end closed, the bottom of at that the placenta, which shreds pedestal,
Magnetic force shear knife 16 is set on bottom in seat 15；
Hematopoietic cell/the tissue collector includes 17,70 microns of cone-shaped collection liquid receiver 18, collection liquid water conservancy diversion of slipping through the net
Pipe 19, collection liquid emulsion tube 20 and collection liquid flow-stop valve 21；70 microns are slipped through the net 18 settings in cone-shaped collection liquid receiver 17,
Collection liquid diversion pipe 19 is arranged in 17 bottom end of cone-shaped collection liquid receiver, and 19 end seal of collection liquid diversion pipe connects collection liquid latex
Collection liquid flow-stop valve 21 is arranged in pipe 20 on collection liquid emulsion tube 20；
When multi-functional placenta tissue processing unit is used to clean placenta, 8 lower end of processor cylinder of placenta processing unit
It links together with 10 threaded upper ends of taper treatment fluid receiver of cleaning solution receiver, it will in the form of 8 traverse of processor cylinder
Connecting tube 6 and cleaning solution diversion pipe 12 are respectively placed in the first belt 1 of multifunctional motor-driven bracket and the curved portion of the second belt 3
On；
When multi-functional placenta tissue processing unit is used to be crushed placenta, 8 lower end of processor cylinder of placenta processing unit
15 threaded upper ends of pedestal for shredding pedestal with placenta link together, and are connect with pedestal 15 and 2 face of electromagnetic mixer with platform
Touching form is placed on multifunctional motor-driven bracket；
When multi-functional placenta tissue processing unit is used for placenta hematopoietic cell/tissue collecting, the place of placenta processing unit
Reason 8 lower end of device cylinder links together with 17 threaded upper ends of hematopoietic cell/tissue collector cone-shaped collection liquid receiver, with place
Connecting tube 6 and collection liquid diversion pipe 19 are respectively placed in 1 He of the first belt of multifunctional motor-driven bracket by reason 8 traverse form of device cylinder
In the curved portion of second belt 3.
Specific embodiment two：The difference of present embodiment and specific embodiment one is：First belt, 1 arc
The diameter in portion is 20cm, and 3 curved portion diameter of the second belt is 20cm.Other are same as the specific embodiment one.
Specific embodiment three：One of present embodiment and specific embodiment one or two difference are：It is described with flat
The platform diameter of the electromagnetic mixer 2 of platform is 20cm.Other with specific embodiment one or two it is identical.
Specific embodiment four：One of present embodiment and specific embodiment one to three difference are：The processor
Internal screw thread is arranged in 7 lower end of lid, and diameter is 20cm；The upper and lower of processor cylinder 8 is respectively provided with external screw thread, and diameter is 20cm, high
For 25cm.Other are identical with specific embodiment one to three.
Specific embodiment five：One of present embodiment and specific embodiment one to four difference are：At the taper
It manages 10 upper end of liquid receiver and internal screw thread is set, and diameter is 20cm；Internal screw thread is arranged in 17 upper end of cone-shaped collection liquid receiver, and straight
Diameter is 20cm.Other are identical with specific embodiment one to four.
Specific embodiment six：One of present embodiment and specific embodiment one to five difference are：Described two gears
Plate 9 is rectangle, and a length of 10cm, width 4cm.Other are identical with specific embodiment one to five.
Specific embodiment seven：One of present embodiment and specific embodiment one to six difference are：The pedestal 15
Upper end be arranged internal screw thread, and diameter be 20cm.Other are identical with specific embodiment one to six.
Specific embodiment eight：One of present embodiment and specific embodiment one to seven difference are：The magnetic force is cut
Cutter 16 is in right-angled intersection, and a length of 8cm, a height of 3cm.Other are identical with specific embodiment one to seven.
The content of the present invention is not limited only to the content of the respective embodiments described above, one of them or several specific embodiments
Combination the purpose of utility model equally also may be implemented.
Using following verification experimental verifications effect of the present invention
Embodiment 1：A kind of multi-functional placenta tissue processing unit, it include multifunctional motor-driven bracket, placenta processing unit,
Cleaning solution receiver, placenta shred pedestal and hematopoietic cell/tissue collector,
The multifunctional motor-driven bracket includes the first belt 1, the electromagnetic mixer 2 with platform, the second belt 3 and electronic
Controller 4, the electromagnetic mixer 2 with platform are arranged on electric controller 4, and the first belt 1 and the second belt 3 are arranged in electricity
4 two sides of movement controller, are respectively provided with curved portion on the first belt 1 and the second belt 3, and the first belt 1 and the second belt 3 are synchronous inverse
Hour hands axially rotate, but the first belt 1 and the second belt 3 are moved up and down in reversed；
The placenta processing unit includes closing silicon rubber cup 5, connecting tube 6, processor lid 7, processor cylinder 8 and two gears
Plate 9, the setting of connecting tube 6 is at the top of processor lid 7, the port setting closing silicon rubber cup 5 of connecting tube 6, the lower end of processor lid 7 with
8 upper end of processor cylinder is together by a threaded connection, and two baffles 9 are oppositely arranged on 8 inner wall of processor cylinder；
The cleaning solution receiver include 10,200 microns of taper treatment fluid receiver 11, cleaning solution diversion pipes 12 of slipping through the net,
Cleaning solution emulsion tube 13 and 14,200 microns of cleaning solution flow-stop valve slip through the net 11 settings in taper treatment fluid receiver 10, in taper
Cleaning solution diversion pipe 12 is arranged in 10 bottom end for the treatment of fluid receiver, and 12 end seal of cleaning solution diversion pipe connects cleaning solution emulsion tube
13, cleaning solution flow-stop valve 14 is set on cleaning solution emulsion tube 13；
It includes pedestal 15 and magnetic force shear knife 16,15 upper end opening of pedestal, lower end closed, the bottom of at that the placenta, which shreds pedestal,
Magnetic force shear knife 16 is set on bottom in seat 15；
Hematopoietic cell/the tissue collector includes 17,70 microns of cone-shaped collection liquid receiver 18, collection liquid water conservancy diversion of slipping through the net
Pipe 19, collection liquid emulsion tube 20 and collection liquid flow-stop valve 21；70 microns are slipped through the net 18 settings in cone-shaped collection liquid receiver 17,
Collection liquid diversion pipe 19 is arranged in 17 bottom end of cone-shaped collection liquid receiver, and 19 end seal of collection liquid diversion pipe connects collection liquid latex
Collection liquid flow-stop valve 21 is arranged in pipe 20 on collection liquid emulsion tube 20；
When multi-functional placenta tissue processing unit is used to clean placenta, 8 lower end of processor cylinder of placenta processing unit
It links together with 10 threaded upper ends of taper treatment fluid receiver of cleaning solution receiver, it will in the form of 8 traverse of processor cylinder
Connecting tube 6 and cleaning solution diversion pipe 12 are respectively placed in the first belt 1 of multifunctional motor-driven bracket and the curved portion of the second belt 3
On；
When multi-functional placenta tissue processing unit is used to be crushed placenta, 8 lower end of processor cylinder of placenta processing unit
15 threaded upper ends of pedestal for shredding pedestal with placenta link together, and are connect with pedestal 15 and 2 face of electromagnetic mixer with platform
Touching form is placed on multifunctional motor-driven bracket；
When multi-functional placenta tissue processing unit is used for placenta hematopoietic cell/tissue collecting, the place of placenta processing unit
Reason 8 lower end of device cylinder links together with 17 threaded upper ends of hematopoietic cell/tissue collector cone-shaped collection liquid receiver, with place
Connecting tube 6 and collection liquid diversion pipe 19 are respectively placed in 1 He of the first belt of multifunctional motor-driven bracket by reason 8 traverse form of device cylinder
In the curved portion of second belt 3.
The diameter of first belt, 1 curved portion described in the present embodiment is 20cm, and 3 curved portion diameter of the second belt is 20cm.
The platform diameter of electromagnetic mixer 2 described in the present embodiment with platform is 20cm.
Internal screw thread is arranged in 7 lower end of processor lid described in the present embodiment, and diameter is 20cm；Processor cylinder 8 up and down
External screw thread is set, and diameter is 20cm, a height of 25cm.
Internal screw thread is arranged in 10 upper end of taper treatment fluid receiver described in the present embodiment, and diameter is 20cm；Cone-shaped collection liquid
Internal screw thread is arranged in 17 upper end of receiver, and diameter is 20cm.
The described two baffles 9 of the present embodiment are rectangle, and a length of 10cm, width 4cm.
Internal screw thread is arranged in the upper end of pedestal 15 described in the present embodiment, and diameter is 20cm.
Magnetic force shear knife 16 described in the present embodiment is in right-angled intersection, and a length of 8cm, a height of 3cm.
Embodiment 2：Using multi-functional placenta tissue processing unit to placenta carry out tissue treatment method, specifically by with
What lower step was completed：
One, placenta is cleaned：
1., on 8 lower end of processor cylinder of placenta processing unit and the taper treatment fluid receiver 10 of cleaning solution receiver
End is threaded togather, and the 500mL placenta of aseptic collection is put into processor cylinder 8,250mL sterile saline is led to
It crosses closing silicon rubber cup 5 to inject in processor cylinder 8, encapsulation process device cylinder 8；
2., in the form of 8 traverse of processor cylinder connecting tube 6 and cleaning solution diversion pipe 12 be respectively placed in multifunctional motor-driven branch
On first belt 1 of frame and the curved portion of the second belt 3；Electric controller 4 is opened, makes processor cylinder 8 with the speed of 50rpm
It rotates counterclockwise, simultaneous processor cylinder 8 vertically moves, and the second belt 3 moves down when the first belt 1 moves up, and washes
Wash 5min；
3., remove processor cylinder 8 from multifunctional motor-driven bracket, and placed in the form of upper vertically by closing silicon rubber cup 5,
Cleaning solution flow-stop valve 14 is opened, cleaning solution is excluded, cleaning solution flow-stop valve 14 is closed, 250mL sterile saline is passed through into closing
Silicon rubber cup 5 injects in processor cylinder 8, encapsulation process device cylinder 8；
4., in the form of 8 traverse of processor cylinder connecting tube 6 and cleaning solution diversion pipe 12 be respectively placed in multifunctional motor-driven branch
On first belt 1 of frame and the curved portion of the second belt 3；Electric controller 4 is opened, makes processor cylinder 8 with the speed of 50rpm
It rotates counterclockwise, simultaneous processor cylinder 8 vertically moves, and the second belt 3 moves down when the first belt 1 moves up, and washes
Wash 5min；
5., remove processor cylinder 8 from multifunctional motor-driven bracket, and placed in the form of upper vertically by closing silicon rubber cup 5,
Cleaning solution flow-stop valve 14 is opened, cleaning solution is excluded, closes cleaning solution flow-stop valve 14, the alcohol for being 75% by 250mL mass fraction
It is injected in processor cylinder 8 by closing silicon rubber cup 5, encapsulation process device cylinder 8；
6., in the form of 8 traverse of processor cylinder connecting tube 6 and cleaning solution diversion pipe 12 be respectively placed in multifunctional motor-driven branch
On first belt 1 of frame and the curved portion of the second belt 3；Electric controller 4 is opened, makes processor cylinder 8 with the speed of 50rpm
It rotates counterclockwise, simultaneous processor cylinder 8 vertically moves, and the second belt 3 moves down when the first belt 1 moves up, and soaks
Steep 5min；
7., remove processor cylinder 8 from multifunctional motor-driven bracket, and placed in the form of upper vertically by closing silicon rubber cup 5,
Cleaning solution flow-stop valve 14 is opened, the alcohol after excluding disinfection, by 250mL sterile saline by closing at 5 injection of silicon rubber cup
It manages in device cylinder 8, encapsulation process device cylinder 8；
8., in the form of 8 traverse of processor cylinder connecting tube 6 and cleaning solution diversion pipe 12 be respectively placed in multifunctional motor-driven branch
On first belt 1 of frame and the curved portion of the second belt 3；Electric controller 4 is opened, makes processor cylinder 8 with the speed of 50rpm
It rotates counterclockwise, simultaneous processor cylinder 8 vertically moves, and the second belt 3 moves down when the first belt 1 moves up, and washes
Wash 5min；
9., remove processor cylinder 8 from multifunctional motor-driven bracket, and placed in the form of upper vertically by closing silicon rubber cup 5,
Cleaning solution flow-stop valve 14 is opened, cleaning solution is excluded, closes cleaning solution flow-stop valve 14；
Two, it is crushed placenta：The pedestal 15 that placenta shreds pedestal replaces the taper treatment fluid receiver 10 of cleaning solution receiver
It is threaded togather with the processor cylinder 8 of placenta processing unit, 4 DEG C of 100mL of sterile saline is passed through into closing silicon
Capsule 5 injects in processor cylinder 8, is then placed in more function in the form of pedestal 15 and 2 face contact of electromagnetic mixer with platform
On energy electric bracket, the electromagnetic mixer 2 for having platform is opened, driving magnetic force shear knife 16 rotates, and revolving speed 90rpm is handled
5min；
Three, placental hematopoietic stem cell is collected：
1., hematopoietic cell/tissue collector cone-shaped collection liquid receiver 17 instead of placenta shred the pedestal 15 of pedestal with
The processor cylinder 8 of placenta processing unit is threaded togather, and is placed in the form of upper vertically by closing silicon rubber cup 5, is opened and receive
Liquid collecting flow-stop valve 21 collects the single cell suspension for being rich in candidate stem cell, closes collection liquid flow-stop valve 21；
2., by 200mL placenta tissue enzymolysis liquid by closing silicon rubber cup 5 inject processor cylinder 8 in, encapsulation process device cylinder
Then connecting tube 6 and collection liquid diversion pipe 19 are respectively placed in multifunctional motor-driven bracket in the form of 8 traverse of processor cylinder by body 8
The first belt 1 and the second belt 3 curved portion on；Electric controller 4 is opened, keeps processor cylinder 8 inverse with the speed of 50rpm
Hour hands rotation, simultaneous processor cylinder 8 vertically move, and the second belt 3 moves down when the first belt 1 moves up, and handle
10min, removes processor cylinder 8 from multifunctional motor-driven bracket, and is placed in the form of upper vertically by closing silicon rubber cup 5, opens
Collection liquid flow-stop valve 21 collects the single cell suspension for being rich in candidate stem cell；Solvent is slow for PBS in the placenta tissue enzymolysis liquid
Fliud flushing, solute are low molecular weight heparin sodium injection, collagenase type I, II Collagenase Type, IV Collagenase Type and trypsase-
EDTA, and the concentration of low molecular weight heparin sodium injection is 15IU/mL, the concentration of collagenase type I is 0.2mg/mL, II Collagen Type VI
The concentration of enzyme is 0.2mg/mL, and the concentration of IV Collagenase Type is 0.2mg/mL, and the concentration of trypsase-EDTA is 0.1mg/mL；
3., by 200mL placenta tissue enzymolysis liquid by closing silicon rubber cup 5 inject processor cylinder 8 in, encapsulation process device cylinder
Then connecting tube 6 and collection liquid diversion pipe 19 are respectively placed in multifunctional motor-driven bracket in the form of 8 traverse of processor cylinder by body 8
The first belt 1 and the second belt 3 curved portion on；Electric controller 4 is opened, keeps processor cylinder 8 inverse with the speed of 50rpm
Hour hands rotation, simultaneous processor cylinder 8 vertically move, and the second belt 3 moves down when the first belt 1 moves up, and handle
10min, removes processor cylinder 8 from multifunctional motor-driven bracket, and is placed in the form of upper vertically by closing silicon rubber cup 5, opens
Collection liquid flow-stop valve 21 collects the single cell suspension for being rich in candidate stem cell；Solvent is slow for PBS in the placenta tissue enzymolysis liquid
Fliud flushing, solute are low molecular weight heparin sodium injection, collagenase type I, II Collagenase Type, IV Collagenase Type and trypsase-
EDTA, and the concentration of low molecular weight heparin sodium injection is 15IU/mL, the concentration of collagenase type I is 0.2mg/mL, II Collagen Type VI
The concentration of enzyme is 0.2mg/mL, and the concentration of IV Collagenase Type is 0.2mg/mL, and the concentration of trypsase-EDTA is 0.1mg/mL；
4., by 200mL PBS buffer solution by closing silicon rubber cup 5 inject processor cylinder 8 in, encapsulation process device cylinder 8,
Connecting tube 6 and collection liquid diversion pipe 19 are respectively placed in the first skin of multifunctional motor-driven bracket in the form of 8 traverse of processor cylinder
With 1 and second belt 3 curved portion on；Electric controller 4 is opened, turns processor cylinder 8 counterclockwise with the speed of 50rpm
It is dynamic, handle 5min；It removes processor cylinder 8 from multifunctional motor-driven bracket, and is put in the form of upper vertically by closing silicon rubber cup 5
It sets, opens collection liquid flow-stop valve 21, collect the single cell suspension for being rich in candidate stem cell；
5., merge step 3 1.~single cell suspension rich in candidate stem cell 4. collected, that is, complete placenta tissue enzyme
Solution separation stem cell is extracted.
It repeats embodiment 2 to operate, obtains 100 parts of single cell suspensions for being rich in candidate stem cell；Using existing conventional method,
It is same to obtain 100 parts of single cell suspensions for being rich in candidate stem cell using the 500mL placenta of aseptic collection as initial feed；Statistics
Karyocyte harvests total amount, and it is (1-2) × 10 that 2 karyocyte of embodiment, which harvests total amount,10A ratio is 13.5%, karyocyte
It harvests total amount (2-5) × 1010A ratio is 28.6%, and karyocyte harvests total amount > 5 × 1010A ratio is 57.9%,
CD34 Positive Hematopoietic Stem Cells number average value is up to 3.2 × 108More than a；Conventional method from placenta tissue as " extracted Hematopoietic Stem
New method of the cell for hemopoietic stem cell bank " karyocyte harvest total amount range is (2~4) × 109It is a, CD34 Positive Hematopoietic
Stem cell population is up to 2.8 × 107It is a.
Claims (8)
1. a kind of multi-functional placenta tissue processing unit, it is characterised in that multi-functional placenta tissue processing unit includes multi-functional electricity
Dynamic bracket, placenta processing unit, cleaning solution receiver, placenta shred pedestal and hematopoietic cell/tissue collector；
The multifunctional motor-driven bracket includes the first belt (1), the electromagnetic mixer (2) with platform, the second belt (3) and electricity
Movement controller (4), the electromagnetic mixer (2) with platform are arranged on electric controller (4), the first belt (1) and the second skin
Band (3) is arranged in electric controller (4) two sides, is respectively provided with curved portion, the first skin on the first belt (1) and the second belt (3)
Band (1) rotation axial counterclockwise synchronous with the second belt (3), but the first belt (1) and the second belt (3) are moved up and down in anti-
To；
The placenta processing unit includes closing silicon rubber cup (5), connecting tube (6), processor lid (7), processor cylinder (8) and two
A baffle (9), connecting tube (6) setting is at the top of processor lid (7), port setting closing silicon rubber cup (5) of connecting tube (6), place
The lower end of reason device lid (7) is together by a threaded connection with processor cylinder (8) upper end, and two baffles (9) are oppositely arranged on place
It manages on device cylinder (8) inner wall；
The cleaning solution receiver includes taper treatment fluid receiver (10), 200 microns of slip through the net (11), cleaning solution diversion pipes
(12), cleaning solution emulsion tube (13) and cleaning solution flow-stop valve (14), 200 microns of (11) settings of slipping through the net are in taper treatment fluid receiver
(10) in, cleaning solution diversion pipe (12) are set in taper treatment fluid receiver (10) bottom end, cleaning solution diversion pipe (12) end is close
Cleaning solution flow-stop valve (14) are arranged on cleaning solution emulsion tube (13) in envelope connection cleaning solution emulsion tube (13)；
It includes pedestal (15) and magnetic force shear knife (16) that the placenta, which shreds pedestal, pedestal (15) upper end opening, lower end closed,
Magnetic force shear knife (16) are set on pedestal (15) interior bottom；
Hematopoietic cell/the tissue collector includes cone-shaped collection liquid receiver (17), 70 microns of slip through the net (18), collection liquid water conservancy diversion
Manage (19), collection liquid emulsion tube (20) and collection liquid flow-stop valve (21)；70 microns of (18) settings of slipping through the net are received in cone-shaped collection liquid
In device (17), collection liquid diversion pipe (19) are set in cone-shaped collection liquid receiver (17) bottom end, collection liquid diversion pipe (19) end
It is tightly connected collection liquid emulsion tube (20), collection liquid flow-stop valve (21) is set on collection liquid emulsion tube (20)；
When multi-functional placenta tissue processing unit for clean placenta when, processor cylinder (8) lower end of placenta processing unit with
Taper treatment fluid receiver (10) threaded upper ends of cleaning solution receiver link together, in the form of processor cylinder (8) traverse
Connecting tube (6) and cleaning solution diversion pipe (12) are respectively placed in the first belt (1) and the second belt (3) of multifunctional motor-driven bracket
Curved portion on；
When multi-functional placenta tissue processing unit for be crushed placenta when, processor cylinder (8) lower end of placenta processing unit with
Pedestal (15) threaded upper ends that placenta shreds pedestal link together, with pedestal (15) and with the electromagnetic mixer (2) of platform
Face contact form is placed on multifunctional motor-driven bracket；
When multi-functional placenta tissue processing unit is used for placenta hematopoietic cell/tissue collecting, the processor of placenta processing unit
Cylinder (8) lower end links together with hematopoietic cell/tissue collector cone-shaped collection liquid receiver (17) threaded upper ends, with place
Connecting tube (6) and collection liquid diversion pipe (19) are respectively placed in the first of multifunctional motor-driven bracket by device cylinder (8) the traverse form of managing
In the curved portion of belt (1) and the second belt (3).
2. a kind of multi-functional placenta tissue processing unit according to claim 1, it is characterised in that first belt (1)
The diameter of curved portion is 20cm, and the second belt (3) curved portion diameter is 20cm.
3. a kind of multi-functional placenta tissue processing unit according to claim 2, it is characterised in that described with platform
The platform diameter of electromagnetic mixer (2) is 20cm.
4. a kind of multi-functional placenta tissue processing unit according to claim 1, it is characterised in that the processor lid (7)
Internal screw thread is arranged in lower end, and diameter is 20cm；The upper and lower of processor cylinder (8) is respectively provided with external screw thread, and diameter is 20cm, a height of
25cm。
5. a kind of multi-functional placenta tissue processing unit according to claim 4, it is characterised in that the taper treatment fluid
Internal screw thread is arranged in receiver (10) upper end, and diameter is 20cm；Internal screw thread is arranged in cone-shaped collection liquid receiver (17) upper end, and straight
Diameter is 20cm.
6. a kind of multi-functional placenta tissue processing unit according to claim 4, it is characterised in that described two baffles (9)
It is rectangle, and a length of 10cm, width 4cm.
7. a kind of multi-functional placenta tissue processing unit according to claim 4, it is characterised in that the pedestal (15)
Internal screw thread is arranged in upper end, and diameter is 20cm.
8. a kind of multi-functional placenta tissue processing unit according to claim 7, it is characterised in that the magnetic force shear knife
It (16) is in right-angled intersection, and a length of 8cm, a height of 3cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820486184.0U CN208166992U (en) | 2018-04-08 | 2018-04-08 | A kind of multi-functional placenta tissue processing unit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820486184.0U CN208166992U (en) | 2018-04-08 | 2018-04-08 | A kind of multi-functional placenta tissue processing unit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208166992U true CN208166992U (en) | 2018-11-30 |
Family
ID=64373610
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820486184.0U Active CN208166992U (en) | 2018-04-08 | 2018-04-08 | A kind of multi-functional placenta tissue processing unit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208166992U (en) |
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2018
- 2018-04-08 CN CN201820486184.0U patent/CN208166992U/en active Active
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