CN106432476A - Signal compensation type temperature monitoring system for extracting fish skin collagen through hydrolysis method - Google Patents
Signal compensation type temperature monitoring system for extracting fish skin collagen through hydrolysis method Download PDFInfo
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- CN106432476A CN106432476A CN201610823333.3A CN201610823333A CN106432476A CN 106432476 A CN106432476 A CN 106432476A CN 201610823333 A CN201610823333 A CN 201610823333A CN 106432476 A CN106432476 A CN 106432476A
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- amplifier
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- resistance
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- audion
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
Abstract
The invention discloses a signal compensation type temperature monitoring system for extracting fish skin collagen through a hydrolysis method. The system is characterized by mainly comprising an amplifier P1, a capacitor C2 and a capacitor C3, wherein a positive pole of the capacitor C3 is connected with the positive pole of the amplifier P1, and a negative pole of the capacitor C3 is connected with an output end of the amplifier P1. The signal compensation type temperature monitoring system disclosed by the invention is capable of filtering the interfering noise in a detected signal, so as to eliminate the influence of the interfering noise; and the influence of the nonlinearity of a temperature sensor on water temperature monitoring can be eliminated, the temperature monitoring precision of the system can be increased, a worker can more effectively control the water temperature, and the water temperature can be kept at the optimal temperature, so that the extraction ratio of the fish skin collagen can be increased.
Description
Technical field
The present invention relates to collagen protein extracts field, specifically refer to a kind of Hydrolyze method extraction collagen protein of fish skin signal and mend
Repay formula temperature monitoring system.
Background technology
In recent years, the development and utilization of collagen protein has caused the extensive concern of people.Contain abundant in collagen protein
Glycine, proline and hydroxyproline, also contain abundant physiologically active peptide in its hydrolyzate, these characteristics make collagen protein
And hydrolyzate has a good application prospect in terms of medical treatment, health care and beauty treatment.Shadow due to European bovine spongiform encephalopathy and foot and mouth disease
Ring, queried by people as the safety of the collagen protein of primary raw material using Corii Bovis seu Bubali, Corii Sus domestica, people start with fish skin, fish
The marine organism materials such as bone replace pig, Corii Bovis seu Bubali as the new raw material extracting collagen protein, marine organisms collagen protein and pig, cattle etc.
The collagen protein of terrestrial animal is compared, and the property of its characteristic, composition and solution all exists dramatically different.
It is a kind of common extracting method that hot water extraction method extracts collagen protein of fish skin, through experiment, is extracted using hot water
When method extracts collagen protein of fish skin, optimal water temperature is 90 DEG C about, and when water temperature is 90 DEG C about, collagen protein of fish skin carries
Take rate highest.In order to ensure water temperature is able to maintain that people are generally supervised to water temperature using temperature monitoring system at 90 DEG C about
Survey, when water temperature over-high or too low when then in time water temperature is adjusted, so that it is guaranteed that the extraction ratio of collagen protein of fish skin.However,
The temperature monitoring system being adopted when extracting to collagen protein of fish skin at present is not high to water temperature detection precision, has had a strong impact on glue
The extraction of former albumen.
Content of the invention
It is an object of the invention to overcoming the temperature monitoring system pair being adopted when extracting at present to collagen protein of fish skin
The not high defect of water temperature detection precision, provides a kind of Hydrolyze method to extract collagen protein of fish skin signal compensation formula temperature monitoring system
System.
The purpose of the present invention is achieved through the following technical solutions:Hydrolyze method extracts collagen protein of fish skin signal compensation formula temperature
Degree monitoring system, mainly by amplifier P1, electric capacity C2, positive pole is connected with the positive pole of amplifier P1, negative pole and amplifier P1
The electric capacity C3 that outfan is connected, the diode D4 that N pole is connected with the positive pole of amplifier P1, P pole is grounded after resistance R11,
One end is connected with the negative pole of amplifier P1, the resistance R10 of other end ground connection, and N pole is connected with the positive pole of amplifier P1, negative pole
The diode D5 being connected with the negative pole of amplifier P1, the compensation circuit being connected with the positive pole of electric capacity C2, respectively with compensation electricity
The temperature collection circuit that road is connected with the negative pole of amplifier P1, the power circuit being connected with temperature collection circuit, Yi Jiyu
The amplifying circuit composition that the outfan of amplifier P1 is connected;The negative pole of described electric capacity C2 is connected with the negative pole of amplifier P1.
Further, described compensation circuit is by amplifier P4, amplifier P5, amplifier P6, positive pole after resistance R16 with put
The electric capacity C6 that big device P4, negative pole are sequentially grounded after resistance R17 and potentiometer R21, is serially connected in positive pole and the output of amplifier P4
Resistance R18 between end, the electric capacity that positive pole is connected with the positive pole of amplifier P4, negative pole is connected with the outfan of amplifier P5
C7, is serially connected in the resistance R19 between the outfan of amplifier P5 and positive pole, and one end is connected with the negative pole of amplifier P5, another
Resistance R20, the potentiometer R22 that N pole is connected with the outfan of amplifier P4, P pole is grounded after potentiometer R22 of end ground connection,
Positive pole is connected with the outfan of amplifier P4, negative pole is connected with the positive pole of amplifier P6 electric capacity C8, positive pole and amplifier
The electric capacity C9 that the positive pole of P6 is connected, negative pole is connected with the outfan of amplifier P6, and be serially connected in electric capacity C9 positive pole and
Resistance R23 composition between the control end of potentiometer R21;The positive pole of described electric capacity C6 is connected with temperature collection circuit;Described
The positive pole of amplifier P5 is connected with the control end of potentiometer R22;The minus earth of described amplifier P6, its output capacitor C2
Positive pole be connected.
By audion VT1, N pole is connected described power circuit with the base stage of audion VT1, the sending out of P pole and audion VT1
The Zener diode D1 that emitter-base bandgap grading is connected, the resistance R2 being in parallel with Zener diode D1, and the base of one end and audion VT1
Pole is connected, the emitter stage of the other end and audion VT1 is collectively forming the resistance R1 composition of power output end;Described audion
The colelctor electrode of VT1 is connected with temperature collection circuit.
Described temperature collection circuit by audion VT2, one end is connected with the emitter stage of audion VT2 after resistance R8,
The resistance R5 that the other end is sequentially connected with the colelctor electrode of audion VT2 after resistance R7 through resistance R6, P pole and audion VT1
Colelctor electrode be connected, diode D3 that N pole is connected with the junction point of resistance R7 and resistance R6, the P of positive pole and diode D3
The base stage of the electric capacity C1 that pole is connected, negative pole is connected with the base stage of audion VT2 after potentiometer R4, N pole and audion VT2
The Zener diode D2 that while being connected, ground connection, P pole are connected with the positive pole of electric capacity C1 after resistance R3, and one end and three
The resistance R9 group that the colelctor electrode of pole pipe VT2 is connected, the other end is connected with the base stage of audion VT2 after temperature sensor B
Become;Described resistance R8 is connected with the base stage of audion VT2 with the junction point of resistance R9, the connection of described resistance R5 and resistance R6
Point is connected with the negative pole of amplifier P1 and the control end of potentiometer R4 respectively, the colelctor electrode of described audion VT2 and electric capacity C6
Positive pole be connected.
By amplifier P2, amplifier P3, one end is connected described amplifying circuit with the positive pole of amplifier P2, other end ground connection
Resistance R12, be serially connected in the resistance R13 between the positive pole of amplifier P2 and outfan, P pole is connected with the outfan of amplifier P1
Connect, diode D6 that N pole is connected with the negative pole of amplifier P2, positive pole is connected with the outfan of amplifier P1, negative pole with put
The electric capacity C4 that the negative pole of big device P3 is connected, is serially connected in the resistance R14 between the positive pole of amplifier P3 and outfan, is serially connected in and puts
Resistance R15 between the outfan of big device P2 and the outfan of amplifier P3, and positive pole is connected with the outfan of amplifier P3
Connect, negative pole forms as the electric capacity C5 of outfan.
Described audion VT2 is 3CG14 type audion.
The present invention compared with the prior art, has advantages below and beneficial effect:
(1) present invention can filter to the interference noise in detection signal, thus exclusive PCR effect of noise, carries
The temperature monitoring precision of the high present invention, makes staff can preferably water temperature be controlled, so that water temperature is maintained optimal
Temperature, thus improve the extraction ratio of collagen protein of fish skin.
(2) present invention can eliminate the non-linear impact to temperature detection of temperature sensor, thus improving the present invention couple
The precision of water temperature detection.
Brief description
Fig. 1 is the overall structure figure of the present invention.
Fig. 2 is the structure chart of the compensation circuit of the present invention.
Specific embodiment
With reference to embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited to
This.
Embodiment
As shown in figure 1, the present invention is mainly by amplifier P1, electric capacity C2, positive pole is connected with the positive pole of amplifier P1, negative pole
The electric capacity C3 being connected with the outfan of amplifier P1, N pole is connected with the positive pole of amplifier P1, P pole is grounded after resistance R11
Diode D4, one end is connected with the negative pole of amplifier P1, the resistance R10 of other end ground connection, the positive pole of N pole and amplifier P1
Be connected, diode D5 that negative pole is connected with the negative pole of amplifier P1, the compensation circuit being connected with the positive pole of electric capacity C2, point
The temperature collection circuit not being connected with the negative pole of compensation circuit and amplifier P1, the power supply electricity being connected with temperature collection circuit
Road, and the amplifying circuit composition being connected with the outfan of amplifier P1.The negative pole of described electric capacity C2 is negative with amplifier P1's
Pole is connected.
This amplifier P1, electric capacity C3, diode D4, diode D5 and electric capacity C2 form a low-pass filter circuit, should
Low-pass filter circuit can filter to the interfering noise signal in the detection signal of temperature collection circuit output, thus improving
The cleannes of detection signal, due to eliminating the impact of interfering noise signal, greatly improve the water temperature detection essence of the present invention
Degree.This amplifier P1 adopts LM102 type amplifier, and the capacitance of electric capacity C2 and electric capacity C3 is 100 μ F, resistance R11 and resistance R10
Resistance be 10K Ω, diode D4 and diode D5 and be 1N4001 type diode.
Described power circuit can provide constant electric current, and it is by audion VT1, resistance R1, resistance R2 and voltage stabilizing two
Pole pipe D1 forms.
The N pole of this Zener diode D1 is connected with the base stage of audion VT1, and P pole is connected with the emitter stage of audion VT1
Connect.Resistance R2 is in parallel with Zener diode D1.One end of resistance R1 is connected with the base stage of audion VT1, the other end and three
The emitter stage of pole pipe VT1 is collectively forming power output end.The colelctor electrode of described audion VT1 is connected with temperature collection circuit.
This audion VT1 is 9013 type audions, and the resistance of resistance R1 is 2K Ω, and the resistance of resistance R2 is 10K Ω, two poles
Pipe D1 is 1N4728 type diode.
This temperature collection circuit can export corresponding voltage signal to low-pass filter circuit according to the water temperature collecting, its
By audion VT2, resistance R3, potentiometer R4, resistance R5, resistance R6, resistance R7, resistance R8, resistance R9, electric capacity C1, temperature passes
Sensor B, Zener diode D2 and diode D3 form.
During connection, one end of resistance R5 is connected with the emitter stage of audion VT2 after resistance R8, and the other end is sequentially through electricity
Resistance R6 is connected with the colelctor electrode of audion VT2 with after resistance R7.The P pole of diode D3 is connected with the colelctor electrode of audion VT1
Connect, N pole is connected with the junction point of resistance R7 and resistance R6.The positive pole of electric capacity C1 is connected with the P pole of diode D3, negative pole warp
It is connected with the base stage of audion VT2 after potentiometer R4.The N pole of Zener diode D2 is connected with the base stage of audion VT2
It is grounded, P pole is connected with the positive pole of electric capacity C1 after resistance R3 simultaneously.The colelctor electrode phase of one end of resistance R9 and audion VT2
Connect, the other end is connected with the base stage of audion VT2 after temperature sensor B.The junction point of described resistance R8 and resistance R9
It is connected with the base stage of audion VT2, junction point negative pole and the current potential with amplifier P1 respectively of described resistance R5 and resistance R6
The control end of device R4 is connected, and the colelctor electrode of described audion VT2 is connected with compensation circuit.
This temperature sensor B is used for gathering water temperature, and it need to be arranged in water, and it is limited that it reaches refrigeration plant using Foshan City's illuminate
The SEN01959 type cooling-water temperature sensor that company produces.This audion VT2, resistance R5, resistance R6, resistance R7, resistance R8 and electricity
Resistance R9 collectively constitutes a bridge circuit, and this bridge circuit is used for being converted into the resistance change rate in temperature sensor accordingly
Voltage output.The maximum value of this potentiometer R4 is 100K Ω, and the resistance of resistance R3 is 2K Ω, resistance R5, resistance R6, resistance R7
And the resistance of resistance R8 is 2.2K Ω, the resistance of resistance R9 is then 650 Ω, model 1N4728 of Zener diode D2,
Model 1N4001 of diode D3, the capacitance of electric capacity C1 is 2.5 μ F.Described audion VT2 is 3CG14 type audion.
In addition, this amplifying circuit is by amplifier P2, amplifier P3, resistance R12, resistance R13, resistance R14, resistance R15, electricity
Hold C4, electric capacity C5 and diode D6 composition.
One end of this resistance R12 is connected with the positive pole of amplifier P2, and the other end is grounded.Resistance R13 is serially connected in amplifier
Between the positive pole of P2 and outfan.The P pole of diode D6 is connected with the outfan of amplifier P1, and N pole is negative with amplifier P2's
Pole is connected.The positive pole of electric capacity C4 is connected with the outfan of amplifier P1, and negative pole is connected with the negative pole of amplifier P3.Resistance
R14 is serially connected between positive pole and the outfan of amplifier P3.Resistance R15 is serially connected in the outfan of amplifier P2 and amplifier P3's
Between outfan.The positive pole of electric capacity C5 is connected with the outfan of amplifier P3, and negative pole as outfan and connects the letter of display
Number input.
Amplifier P2, amplifier P3, resistance R13, resistance R14 and resistance R15 constitute a feedback amplifier, this feedback
Amplifier can be amplified to the voltage signal of low-pass filter circuit output, and resistance R13 and resistance R14 is feedback resistance,
This resistance R13 and resistance R14 can greatly improve the performance of amplifier.Wherein, the model of amplifier P2 and amplifier P3 is equal
For OP07, the resistance of resistance R13 and resistance R14 is 3K Ω, and the resistance of resistance R12 and resistance R15 is 5K Ω, diode D6
For 1N4001 diode, the capacitance of electric capacity C4 and electric capacity C5 is 1 μ F.
As shown in Fig. 2 this compensation circuit can carry out linear compensation to the signal of temperature collection circuit output, it is by amplifying
Device P4, amplifier P5, amplifier P6, resistance R16, resistance R17, resistance R18, resistance R19, resistance R20, potentiometer R21, current potential
Device R22, resistance R23, electric capacity C6, electric capacity C7, electric capacity C8, electric capacity C9 and diode D7 form.
During connection, the positive pole of electric capacity C6 after resistance R16 with amplifier P4, negative pole is sequentially through resistance R17 and potentiometer R21
After be grounded.Resistance R18 is serially connected between positive pole and the outfan of amplifier P4.The positive pole phase of the positive pole of electric capacity C7 and amplifier P4
Connect, negative pole is connected with the outfan of amplifier P5.Resistance R19 is serially connected between outfan and the positive pole of amplifier P5.Electricity
One end of resistance R20 is connected with the negative pole of amplifier P5, and the other end is grounded.The N pole of potentiometer R22 and the outfan of amplifier P4
It is connected, P pole is grounded after potentiometer R22.The positive pole of electric capacity C8 is connected with the outfan of amplifier P4, negative pole and amplifier
The positive pole of P6 is connected.The positive pole of electric capacity C9 is connected with the positive pole of amplifier P6, and negative pole is connected with the outfan of amplifier P6
Connect.Resistance R23 is serially connected between the positive pole of electric capacity C9 and the control end of potentiometer R21.The positive pole of described electric capacity C6 and audion
The colelctor electrode of VT2 is connected.The positive pole of described amplifier P5 is connected with the control end of potentiometer R22.Described amplifier P6's
Minus earth, the positive pole of its output capacitor C2 is connected.
This amplifier P5, electric capacity R19, diode D7, potentiometer R22 and electric capacity C7 form a positive feedback linear compensation
Circuit, this positive feedback linear compensation circuit can to temperature sensor in different temperature points due to resistance rate of change decline and
Lead to measure the part that output signal is reduced or increased, that is, eliminate the non-linear shadow bringing to temperature monitoring of temperature sensor
Ring, thus improving the precision to water temperature detection for the present invention.This potentiometer R21 is zero point regulator potentiometer, and its maximum value is
100KΩ.The maximum value of potentiometer R22 is 100K Ω, resistance R16, resistance R18, resistance R19, resistance R20, resistance R23's
Resistance is 10K Ω, and the resistance of resistance R17 is 22K Ω, and electric capacity C6, the capacitance of electric capacity C7, electric capacity C8 and electric capacity C9 are
2.2 μ F, diode D7 are 1N4001 type diode, and the model of amplifier P4, amplifier P5 and amplifier P6 is OP07.
The present invention can filter to the interference noise in detection signal, thus exclusive PCR effect of noise, simultaneously
Non-linear the impact that water temperature detection is caused because of temperature sensor can also be eliminated, improve the temperature monitoring essence of the present invention
Degree, making staff can preferably water temperature be controlled, making water temperature maintain optimal temperature, thus improving fish skin collagen
The extraction ratio of albumen.
As described above, the present invention just can be realized well.
Claims (6)
1. Hydrolyze method extract collagen protein of fish skin with signal compensation formula temperature monitoring system it is characterised in that main by amplifier
P1, electric capacity C2, the electric capacity C3 that positive pole is connected with the positive pole of amplifier P1, negative pole is connected with the outfan of amplifier P1, N pole
Be connected with the positive pole of amplifier P1, diode D4 that P pole is grounded after resistance R11, one end is connected with the negative pole of amplifier P1
Connect, the other end ground connection resistance R10, N pole is connected with the positive pole of amplifier P1, negative pole is connected with the negative pole of amplifier P1
Diode D5, the compensation circuit being connected with the positive pole of electric capacity C2, it is connected with the negative pole of compensation circuit and amplifier P1 respectively
Temperature collection circuit, the power circuit being connected with temperature collection circuit, and be connected with the outfan of amplifier P1
Amplifying circuit forms;The negative pole of described electric capacity C2 is connected with the negative pole of amplifier P1.
2. Hydrolyze method according to claim 1 extracts collagen protein of fish skin signal compensation formula temperature monitoring system, and it is special
Levy and be, described compensation circuit by amplifier P4, amplifier P5, amplifier P6, positive pole after resistance R16 with amplifier P4, negative
The electric capacity C6 that pole is sequentially grounded after resistance R17 and potentiometer R21, is serially connected in the electricity between the positive pole of amplifier P4 and outfan
Resistance R18, the electric capacity C7 that positive pole is connected with the positive pole of amplifier P4, negative pole is connected with the outfan of amplifier P5, are serially connected in
Resistance R19 between the outfan of amplifier P5 and positive pole, one end is connected with the negative pole of amplifier P5, the electricity of other end ground connection
Resistance R20, N pole is connected with the outfan of amplifier P4, P pole is grounded after potentiometer R22 potentiometer R22, positive pole and amplification
The electric capacity C8 that the outfan of device P4 is connected, negative pole is connected with the positive pole of amplifier P6, the positive pole phase of positive pole and amplifier P6
Connect, the electric capacity C9 that is connected with the outfan of amplifier P6 of negative pole, and be serially connected in the positive pole of electric capacity C9 and potentiometer R21
Resistance R23 composition between control end;The positive pole of described electric capacity C6 is connected with temperature collection circuit;Described amplifier P5 is just
Pole is connected with the control end of potentiometer R22;The minus earth of described amplifier P6, the positive pole of its output capacitor C2 are connected
Connect.
3. Hydrolyze method according to claim 2 extracts collagen protein of fish skin signal compensation formula temperature monitoring system, and it is special
Levy and be, by audion VT1, N pole is connected with the base stage of audion VT1 described power circuit, the sending out of P pole and audion VT1
The Zener diode D1 that emitter-base bandgap grading is connected, the resistance R2 being in parallel with Zener diode D1, and the base of one end and audion VT1
Pole is connected, the emitter stage of the other end and audion VT1 is collectively forming the resistance R1 composition of power output end;Described audion
The colelctor electrode of VT1 is connected with temperature collection circuit.
4. Hydrolyze method according to claim 3 extracts collagen protein of fish skin signal compensation formula temperature monitoring system, and it is special
Levy and be, described temperature collection circuit is connected with the emitter stage of audion VT2, separately by audion VT2, one end after resistance R8
The resistance R5 that one end is sequentially connected with the colelctor electrode of audion VT2 after resistance R7 through resistance R6, P pole is with audion VT1's
The P pole of the diode D3 that colelctor electrode is connected, N pole is connected with the junction point of resistance R7 and resistance R6, positive pole and diode D3
Be connected, electric capacity C1 that negative pole is connected with the base stage of audion VT2 after potentiometer R4, the base stage phase of N pole and audion VT2
The Zener diode D2 that while connection, ground connection, P pole are connected with the positive pole of electric capacity C1 after resistance R3, and one end and three poles
The resistance R9 composition that the colelctor electrode of pipe VT2 is connected, the other end is connected with the base stage of audion VT2 after temperature sensor B;
Described resistance R8 is connected with the base stage of audion VT2 with the junction point of resistance R9, the junction point of described resistance R5 and resistance R6
It is connected with the negative pole of amplifier P1 and the control end of potentiometer R4 respectively, the colelctor electrode of described audion VT2 is with electric capacity C6's
Positive pole is connected.
5. Hydrolyze method according to claim 4 extracts collagen protein of fish skin signal compensation formula temperature monitoring system, and it is special
Levy and be, by amplifier P2, amplifier P3, one end is connected with the positive pole of amplifier P2 described amplifying circuit, other end ground connection
Resistance R12, be serially connected in the resistance R13 between the positive pole of amplifier P2 and outfan, P pole is connected with the outfan of amplifier P1
Connect, diode D6 that N pole is connected with the negative pole of amplifier P2, positive pole is connected with the outfan of amplifier P1, negative pole with put
The electric capacity C4 that the negative pole of big device P3 is connected, is serially connected in the resistance R14 between the positive pole of amplifier P3 and outfan, is serially connected in and puts
Resistance R15 between the outfan of big device P2 and the outfan of amplifier P3, and positive pole is connected with the outfan of amplifier P3
Connect, negative pole forms as the electric capacity C5 of outfan.
6. Hydrolyze method according to claim 5 extracts collagen protein of fish skin signal compensation formula temperature monitoring system, and it is special
Levy and be, described audion VT2 is 3CG14 type audion.
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN110794889A (en) * | 2019-10-29 | 2020-02-14 | 刘洋 | Temperature control system for slice dyeing reaction cabin |
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CN103017217A (en) * | 2012-12-21 | 2013-04-03 | 敦泰科技有限公司 | Micro-wave oven control method, control equipment and micro-wave oven |
CN103342748A (en) * | 2013-07-26 | 2013-10-09 | 中国人民解放军第三军医大学第一附属医院 | Special extraction device for main ingredient collagen II of natural cartilage matrix |
CN105061587A (en) * | 2015-08-26 | 2015-11-18 | 华南理工大学 | Ultrasonic wave aided extraction method suitable for high-viscosity collagen |
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EP0560548B1 (en) * | 1992-03-11 | 1997-05-14 | Sony Corporation | Power control circuit |
CN103017217A (en) * | 2012-12-21 | 2013-04-03 | 敦泰科技有限公司 | Micro-wave oven control method, control equipment and micro-wave oven |
CN103342748A (en) * | 2013-07-26 | 2013-10-09 | 中国人民解放军第三军医大学第一附属医院 | Special extraction device for main ingredient collagen II of natural cartilage matrix |
CN105061587A (en) * | 2015-08-26 | 2015-11-18 | 华南理工大学 | Ultrasonic wave aided extraction method suitable for high-viscosity collagen |
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