CN106432477A - Temperature monitoring system for fish skin collagen extraction using hot water hydrolysis - Google Patents
Temperature monitoring system for fish skin collagen extraction using hot water hydrolysis Download PDFInfo
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- CN106432477A CN106432477A CN201610825302.1A CN201610825302A CN106432477A CN 106432477 A CN106432477 A CN 106432477A CN 201610825302 A CN201610825302 A CN 201610825302A CN 106432477 A CN106432477 A CN 106432477A
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- resistance
- amplifier
- pole
- audion
- outfan
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
Abstract
The invention discloses a temperature monitoring system for fish skin collagen extraction using hot water hydrolysis. The system is characterized by comprising a magnifier P1 and a capacitor C2. A positive electrode of a capacitor C3 is connected with the positive electrode of the magnifier P1. A negative electrode of the capacitor C3 is connected with the negative electrode of the magnifier P1. A N electrode is connected with the positive electrode of the magnifier P1. The system also comprises a diode D4, wherein a P electrode of the diode D4 is grounded through a resistance R11. The system can filter the interference noise in the monitored signals, and thus remove the influences caused by the interference noise. The system enhances the temperature monitoring precision, allow operators to control the water temperature in a more optimized status, make sure the water temperature be kept at the optimal level, and thereby increasing the extraction rate of the fish skin collagen.
Description
Technical field
The present invention relates to collagen protein extracts field, specifically refer to a kind of using hot water Hydrolyze method extraction collagen protein of fish skin
Use temperature monitoring system.
Background technology
In recent years, the development and utilization of collagen protein has caused the extensive concern of people.Containing abundant in collagen protein
Glycine, proline and hydroxyproline, also containing abundant physiologically active peptide in its hydrolyzate, these characteristics make collagen protein
And hydrolyzate is had a good application prospect in terms of medical treatment, health care and beauty.Due to European bovine spongiform encephalopathy and the shadow of foot and mouth disease
Ring, queried by people as the safety of the collagen protein of primary raw material using Corii Bovis seu Bubali, Corii Sus domestica, people start with fish skin, fish
The marine organism materials such as bone replace pig, Corii Bovis seu Bubali as the new raw material for extracting collagen protein, marine organisms collagen protein and pig, cattle etc.
The collagen protein of terrestrial animal is compared, and the property of its characteristic, composition and solution all exists dramatically different.
It is a kind of common extracting method that hot water extraction method extracts collagen protein of fish skin, through experiment, is extracted using hot water
When method extracts collagen protein of fish skin, optimal water temperature is 90 DEG C or so, and when water temperature is 90 DEG C or so, collagen protein of fish skin carries
Take rate highest.In order to ensure water temperature is able to maintain that people are generally supervised to water temperature using temperature monitoring system at 90 DEG C or so
Survey, when water temperature over-high or too low when then in time water temperature is adjusted, so that it is guaranteed that the extraction ratio of collagen protein of fish skin.However,
The temperature monitoring system for being adopted when extracting to collagen protein of fish skin at present is not high to water temperature detection precision, has had a strong impact on glue
The extraction of former albumen.
Content of the invention
It is an object of the invention to overcoming the temperature monitoring system pair for being adopted when extracting at present to collagen protein of fish skin
The not high defect of water temperature detection precision, provides a kind of using hot water Hydrolyze method extraction collagen protein of fish skin temperature monitoring system.
The purpose of the present invention is achieved through the following technical solutions：One kind extracts collagen protein of fish skin use using hot water Hydrolyze method
Temperature monitoring system, mainly by amplifier P1, electric capacity C2, positive pole is connected with the positive pole of amplifier P1, negative pole and amplifier P1
Electric capacity C3, the N pole that is connected of outfan be connected with the positive pole of amplifier P1, diode that P pole is grounded after resistance R11
D4, one end is connected with the negative pole of amplifier P1, resistance R10, the N pole of other end ground connection is connected with the positive pole of amplifier P1,
The diode D5 that negative pole is connected with the negative pole of amplifier P1, is connected with the positive pole of electric capacity C2 and the negative pole of amplifier P1 respectively
Temperature collection circuit, the power circuit being connected with temperature collection circuit, and be connected with the outfan of amplifier P1
Amplifying circuit constitutes；The negative pole of the electric capacity C2 is connected with the negative pole of amplifier P1.
Further, the power circuit be connected with the base stage of audion VT1 by audion VT1, N pole, P pole and three poles
The Zener diode D1 that the emitter stage of pipe VT1 is connected, the resistance R2 being in parallel with Zener diode D1, and one end and three poles
The base stage of pipe VT1 is connected, the emitter stage of the other end and audion VT1 is collectively forming the resistance R1 composition of power output end；Institute
The colelctor electrode for stating audion VT1 is connected with temperature collection circuit.
The temperature collection circuit by audion VT2, one end is connected with the emitter stage of audion VT2 after resistance R8,
Resistance R5, P pole and audion VT1 that the other end is sequentially connected with the colelctor electrode of audion VT2 after resistance R7 through resistance R6
Colelctor electrode be connected, diode D3 that N pole is connected with the junction point of resistance R7 and resistance R6, the P of positive pole and diode D3
The base stage of electric capacity C1, the N pole that pole is connected, negative pole is connected with the base stage of audion VT2 after potentiometer R4 and audion VT2
The Zener diode D2 that while being connected, ground connection, P pole are connected with the positive pole of electric capacity C1 after resistance R3, and one end and three
The resistance R9 group that the colelctor electrode of pole pipe VT2 is connected, the other end is connected with the base stage of audion VT2 after temperature sensor B
Become；The resistance R8 is connected with the base stage of audion VT2 with the junction point of resistance R9, the connection of the resistance R5 and resistance R6
Point is connected with the negative pole of amplifier P1 and the control end of potentiometer R4 respectively, the colelctor electrode of the audion VT2 and electric capacity C2
Positive pole be connected.
The amplifying circuit is by amplifier P2, amplifier P3, and one end is connected with the positive pole of amplifier P2, other end ground connection
Resistance R12, resistance R13, the P pole being serially connected between the positive pole of amplifier P2 and outfan is connected with the outfan of amplifier P1
Connect, diode D6 that N pole is connected with the negative pole of amplifier P2, positive pole is connected with the outfan of amplifier P1, negative pole with put
Electric capacity C4, the resistance R14 being serially connected between the positive pole of amplifier P3 and outfan that the negative pole of big device P3 is connected, are serially connected in and put
Big resistance R15 between the outfan of device P2 and the outfan of amplifier P3, and positive pole is connected with the outfan of amplifier P3
Connect, negative pole constitutes as the electric capacity C5 of outfan.
The audion VT2 is 3CG14 type audion.
The present invention compared with the prior art, with advantages below and beneficial effect：
The present invention can be filtered to the interference noise in detection signal, so as to exclusive PCR effect of noise, is improved
The temperature monitoring precision of the present invention, allows staff to be preferably controlled water temperature, makes water temperature maintain optimal temperature
Degree, so as to improve the extraction ratio of collagen protein of fish skin.
Description of the drawings
Fig. 1 is the overall structure figure of the present invention.
Specific embodiment
With reference to embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited to
This.
Embodiment
As shown in figure 1, the present invention is mainly by amplifier P1, electric capacity C2, positive pole is connected with the positive pole of amplifier P1, negative pole
Electric capacity C3, the N pole being connected with the outfan of amplifier P1 is connected with the positive pole of amplifier P1, P pole is grounded after resistance R11
Diode D4, one end is connected with the negative pole of amplifier P1, the positive pole of resistance R10, N pole and the amplifier P1 of other end ground connection
Be connected, diode D5 that negative pole is connected with the negative pole of amplifier P1, negative with the positive pole of electric capacity C2 and amplifier P1 respectively
The temperature collection circuit that pole is connected, the power circuit being connected with temperature collection circuit, and the outfan with amplifier P1
The amplifying circuit composition being connected.The negative pole of the electric capacity C2 is connected with the negative pole of amplifier P1.
Amplifier P1, electric capacity C3, diode D4, diode D5 and electric capacity C2 constitute a low-pass filter circuit, should
Low-pass filter circuit can be filtered to the interfering noise signal in the detection signal of temperature collection circuit output, so as to improve
The cleannes of detection signal, due to eliminating the impact of interfering noise signal, greatly improve the water temperature detection essence of the present invention
Degree.Amplifier P1 adopts LM102 type amplifier, and the capacitance of electric capacity C2 and electric capacity C3 is 100 μ F, resistance R11 and resistance R10
Resistance be 10K Ω, diode D4 and diode D5 and be 1N4001 type diode.
The power circuit can provide constant electric current, and which is by audion VT1, resistance R1, resistance R2 and voltage stabilizing two
Pole pipe D1 constitutes.
The N pole of Zener diode D1 is connected with the base stage of audion VT1, and P pole is connected with the emitter stage of audion VT1
Connect.Resistance R2 is in parallel with Zener diode D1.One end of resistance R1 is connected with the base stage of audion VT1, the other end and three
The emitter stage of pole pipe VT1 is collectively forming power output end.The colelctor electrode of the audion VT1 is connected with temperature collection circuit.
Audion VT1 is 9013 type audions, and the resistance of resistance R1 is 10K Ω for the resistance of 2K Ω, resistance R2, two poles
Pipe D1 is 1N4728 type diode.
The temperature collection circuit can export corresponding voltage signal to low-pass filter circuit according to the water temperature for collecting, its
By audion VT2, resistance R3, potentiometer R4, resistance R5, resistance R6, resistance R7, resistance R8, resistance R9, electric capacity C1, temperature is passed
Sensor B, Zener diode D2 and diode D3 constitute.
During connection, one end of resistance R5 is connected with the emitter stage of audion VT2 after resistance R8, and the other end is sequentially through electricity
Hinder R6 and be connected with the colelctor electrode of audion VT2 after resistance R7.The P pole of diode D3 is connected with the colelctor electrode of audion VT1
Connect, N pole is connected with the junction point of resistance R7 and resistance R6.The positive pole of electric capacity C1 is connected with the P pole of diode D3, negative pole warp
It is connected with the base stage of audion VT2 after potentiometer R4.The N pole of Zener diode D2 is connected with the base stage of audion VT2
While being grounded, P pole is connected with the positive pole of electric capacity C1 after resistance R3.The colelctor electrode phase of one end of resistance R9 and audion VT2
Connection, the other end is connected with the base stage of audion VT2 after temperature sensor B.The junction point of the resistance R8 and resistance R9
It is connected with the base stage of audion VT2, the junction point of the resistance R5 and resistance R6 negative pole and current potential respectively with amplifier P1
The control end of device R4 is connected, and the colelctor electrode of the audion VT2 is connected with the positive pole of electric capacity C2.
Temperature sensor B is used for gathering water temperature, and which need to be arranged in water, and it is limited which reaches refrigeration plant using Foshan City's illuminate
The SEN01959 type cooling-water temperature sensor of company's production.Audion VT2, resistance R5, resistance R6, resistance R7, resistance R8 and electricity
Resistance R9 collectively constitutes a bridge circuit, and the bridge circuit is used for being converted into the resistance change rate in temperature sensor accordingly
Voltage output.The maximum value of potentiometer R4 is 2K Ω for the resistance of 100K Ω, resistance R3, resistance R5, resistance R6, resistance R7
And the resistance of resistance R8 to be the resistance of 2.2K Ω, resistance R9 be then 650 Ω, model 1N4728 of Zener diode D2,
Model 1N4001 of diode D3, the capacitance of electric capacity C1 is 2.5 μ F.The audion VT2 is 3CG14 type audion.
In addition, the amplifying circuit is by amplifier P2, amplifier P3, resistance R12, resistance R13, resistance R14, resistance R15, electricity
Hold C4, electric capacity C5 and diode D6 composition.
One end of resistance R12 is connected with the positive pole of amplifier P2, and the other end is grounded.Resistance R13 is serially connected in amplifier
Between the positive pole of P2 and outfan.The P pole of diode D6 is connected with the outfan of amplifier P1, and N pole is negative with amplifier P2
Pole is connected.The positive pole of electric capacity C4 is connected with the outfan of amplifier P1, and negative pole is connected with the negative pole of amplifier P3.Resistance
R14 is serially connected between the positive pole of amplifier P3 and outfan.Resistance R15 is serially connected in the outfan of amplifier P2 and amplifier P3's
Between outfan.The positive pole of electric capacity C5 is connected with the outfan of amplifier P3, and negative pole as outfan and connects the letter of display
Number input.
Amplifier P2, amplifier P3, resistance R13, resistance R14 and resistance R15 constitute a feedback amplifier, the feedback
Amplifier can be amplified to the voltage signal of low-pass filter circuit output, and resistance R13 and resistance R14 is feedback resistance,
Resistance R13 and resistance R14 can greatly improve the performance of amplifier.Wherein, the model of amplifier P2 and amplifier P3 is equal
For OP07, the resistance of resistance R13 and resistance R14 is 3K Ω, and the resistance of resistance R12 and resistance R15 is 5K Ω, diode D6
For 1N4001 diode, the capacitance of electric capacity C4 and electric capacity C5 is 1 μ F.
The present invention can be filtered to the interference noise in detection signal, so as to exclusive PCR effect of noise, is improved
The temperature monitoring precision of the present invention, allows staff to be preferably controlled water temperature, makes water temperature maintain optimal temperature
Degree, so as to improve the extraction ratio of collagen protein of fish skin.
As described above, the present invention just can be realized well.
Claims (5)
1. a kind of collagen protein of fish skin temperature monitoring system is extracted using hot water Hydrolyze method, it is characterised in that main by amplifying
Device P1, electric capacity C2, positive pole is connected with the positive pole of amplifier P1, negative pole is connected with the outfan of amplifier P1 electric capacity C3, N
The diode D4 that pole is connected with the positive pole of amplifier P1, P pole is grounded after resistance R11, the negative pole phase of one end and amplifier P1
Resistance R10, the N pole that connection, the other end are grounded is connected with the positive pole of amplifier P1, negative pole is connected with the negative pole of amplifier P1
Diode D5, the temperature collection circuit being connected with the positive pole of electric capacity C2 and the negative pole of amplifier P1 respectively, with temperature acquisition
The power circuit that circuit is connected, and the amplifying circuit composition being connected with the outfan of amplifier P1；The electric capacity C2's
Negative pole is connected with the negative pole of amplifier P1.
2. according to claim 1 a kind of using hot water Hydrolyze method extraction collagen protein of fish skin temperature monitoring system, its
It is characterised by, the power circuit is connected with the base stage of audion VT1 by audion VT1, N pole, P pole and audion VT1
The Zener diode D1 that emitter stage is connected, the resistance R2 being in parallel with Zener diode D1, and one end is with audion VT1's
Base stage is connected, the emitter stage of the other end and audion VT1 is collectively forming the resistance R1 composition of power output end；The audion
The colelctor electrode of VT1 is connected with temperature collection circuit.
3. according to claim 2 a kind of using hot water Hydrolyze method extraction collagen protein of fish skin temperature monitoring system, its
Be characterised by, the temperature collection circuit by audion VT2, one end is connected with the emitter stage of audion VT2 after resistance R8,
Resistance R5, P pole and audion VT1 that the other end is sequentially connected with the colelctor electrode of audion VT2 after resistance R7 through resistance R6
Colelctor electrode be connected, diode D3 that N pole is connected with the junction point of resistance R7 and resistance R6, the P of positive pole and diode D3
The base stage of electric capacity C1, the N pole that pole is connected, negative pole is connected with the base stage of audion VT2 after potentiometer R4 and audion VT2
The Zener diode D2 that while being connected, ground connection, P pole are connected with the positive pole of electric capacity C1 after resistance R3, and one end and three
The resistance R9 group that the colelctor electrode of pole pipe VT2 is connected, the other end is connected with the base stage of audion VT2 after temperature sensor B
Become；The resistance R8 is connected with the base stage of audion VT2 with the junction point of resistance R9, the connection of the resistance R5 and resistance R6
Point is connected with the negative pole of amplifier P1 and the control end of potentiometer R4 respectively, the colelctor electrode of the audion VT2 and electric capacity C2
Positive pole be connected.
4. according to claim 3 a kind of using hot water Hydrolyze method extraction collagen protein of fish skin temperature monitoring system, its
It is characterised by, the amplifying circuit is by amplifier P2, amplifier P3, and one end is connected with the positive pole of amplifier P2, another termination
The resistance R12 on ground, is serially connected in the outfan phase of resistance R13, the P pole between the positive pole of amplifier P2 and outfan and amplifier P1
Connection, the diode D6 that is connected with the negative pole of amplifier P2 of N pole, positive pole is connected with the outfan of amplifier P1, negative pole with
The electric capacity C4 that the negative pole of amplifier P3 is connected, the resistance R14 being serially connected between the positive pole of amplifier P3 and outfan, are serially connected in
Resistance R15 between the outfan of the outfan of amplifier P2 and amplifier P3, and the outfan phase of positive pole and amplifier P3
Connection, negative pole constitute as the electric capacity C5 of outfan.
5. according to claim 4 a kind of using hot water Hydrolyze method extraction collagen protein of fish skin temperature monitoring system, its
It is characterised by, the audion VT2 is 3CG14 type audion.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610825302.1A CN106432477A (en) | 2016-09-14 | 2016-09-14 | Temperature monitoring system for fish skin collagen extraction using hot water hydrolysis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610825302.1A CN106432477A (en) | 2016-09-14 | 2016-09-14 | Temperature monitoring system for fish skin collagen extraction using hot water hydrolysis |
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| Publication Number | Publication Date |
|---|---|
| CN106432477A true CN106432477A (en) | 2017-02-22 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610825302.1A Withdrawn CN106432477A (en) | 2016-09-14 | 2016-09-14 | Temperature monitoring system for fish skin collagen extraction using hot water hydrolysis |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0560548B1 (en) * | 1992-03-11 | 1997-05-14 | Sony Corporation | Power control circuit |
| CN103017217A (en) * | 2012-12-21 | 2013-04-03 | 敦泰科技有限公司 | Micro-wave oven control method, control equipment and micro-wave oven |
| CN103342748A (en) * | 2013-07-26 | 2013-10-09 | 中国人民解放军第三军医大学第一附属医院 | Special extraction device for main ingredient collagen II of natural cartilage matrix |
| CN105061587A (en) * | 2015-08-26 | 2015-11-18 | 华南理工大学 | Ultrasonic wave aided extraction method suitable for high-viscosity collagen |
-
2016
- 2016-09-14 CN CN201610825302.1A patent/CN106432477A/en not_active Withdrawn
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0560548B1 (en) * | 1992-03-11 | 1997-05-14 | Sony Corporation | Power control circuit |
| CN103017217A (en) * | 2012-12-21 | 2013-04-03 | 敦泰科技有限公司 | Micro-wave oven control method, control equipment and micro-wave oven |
| CN103342748A (en) * | 2013-07-26 | 2013-10-09 | 中国人民解放军第三军医大学第一附属医院 | Special extraction device for main ingredient collagen II of natural cartilage matrix |
| CN105061587A (en) * | 2015-08-26 | 2015-11-18 | 华南理工大学 | Ultrasonic wave aided extraction method suitable for high-viscosity collagen |
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Application publication date: 20170222 |