CN103342598A - Mushroom stock culture material, and preparation method of culture material - Google Patents
Mushroom stock culture material, and preparation method of culture material Download PDFInfo
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- CN103342598A CN103342598A CN2013102704870A CN201310270487A CN103342598A CN 103342598 A CN103342598 A CN 103342598A CN 2013102704870 A CN2013102704870 A CN 2013102704870A CN 201310270487 A CN201310270487 A CN 201310270487A CN 103342598 A CN103342598 A CN 103342598A
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Abstract
The invention discloses a mushroom stock culture material. The mushroom stock culture material is characterized in that sheep manure particles are taken as materials; cotton seed hulls, soil and rape seed cakes are added as intergranular bridging materials to prepare mushroom stock. The moisture content of the sheep manure particles is 45%; the moisture content of the intergranular bridging materials is 65%. The invention also comprises a preparation method of the culture material. Compared with the wheat species, the mushroom stock culture material has the following advantages: 1, the sheep manure is comprehensive in nutrition, hyphae vigorously grow, and the strain is strong in vitality; 2, a granular sheep strain is less in dosage, and high in work efficiency when being transferred and cultivated; 3, escherichia coli, parasitic ova and weed seeds in the sheep manure are killed in the sterilization link of production of hybrid seeds, and the hygienic condition of a sheep yard is purified; 4, a material channel of the culture material is expanded; the preparation cost of the strain is reduced.
Description
Technical field
Content of the present invention comprises mushroom original seed culture material compatibility, and the making method of this culture material, belongs to edible fungus species and makes the field.
Background technology
Mushroom is global edible fungus culturing kind, and output occupies the edible fungus variety first place.Canned mushroom is one of topmost commercial form of whole world mushroom trade.At present, China oneself become in the world maximum mushroom big producing country and big export country.
Along with the expansion of mushroom cultivated area, the wheat consumption that the production of hybrid seeds is used also increases thereupon, for reducing grain consumption, improves strain quality, must seek the reasonable substitute of the production of hybrid seeds.
Sheep husbandry has history in several thousand in China, and on sheep raising quantity, raising scale, China has stepped into sheep raising big country ranks, and the sheep breeding stock is many, and sheep excrement quantity is big.
The sheep excrement refers to the stool of sheep, and black becomes the ight soil size of sheep as soybean grain, approximate ellipsoidal, and excrement matter is thinner, and nutrient is complete.The sheep excrement contains plant essential macroelement nitrogen, phosphorus, potassium, micro-sulphur, calcium, magnesium, iron, manganese, zinc, molybdenum, boron.
Sheep is herbivore, mainly be that to eat natural grass be main, so Mierocrystalline cellulose hemicellulose (robust fibre) content is higher in the faecal pellet of sheep, and the mushroom-cultivating material is excrement grass substratum, so the very suitable mushroom mycelium growth of sheep excrement.
Utilize the sheep faecal pellet to substitute wheat and make the mushroom original seed, be not only this behave of falling of an Edible Fungi, but also be a circular agriculture and environmental protection cause behave.
Summary of the invention
The present invention has following two aspect contents:
1, a kind of mushroom original seed culture material compatibility
2, the making method of culture material
The technical solution adopted in the present invention is:
A kind of mushroom original seed culture material compatibility has following component and quality proportioning: sheep faecal pellet 85%-90%, and cotton seed hulls 3%-5%, dried earth 3%-5%, rapeseed cake 1.5%, terra alba 3% all is the quality of dry-matter, the proportioning sum is 100%.
The sheep faecal pellet: fresh sheep excrement, standby after drying.Nitrogenous (N) 2.317% of dried sheep excrement, phosphorus (P
2O
3) 0.457%, potassium (K
2O) 1.284%.
Earth: select loam, get the following soil of arable layer 18cm-20cm, the cool solarization broken into pieces when shining to semi-wet, dries standby.
Rapeseed cake: be the byproduct after the vegetable seed oil expression.Rapeseed cake contains crude protein 33%-45%, crude fat 2%-3%, carbohydrate 24%-32%.
Terra alba: be common commercially available product.The potential of hydrogen of buffering culture material.
The making method of culture material comprises the steps:
1. shine material: the 1d-2d that before spice raw material is exposed to the sun, utilize ultraviolet ray to kill the assorted bacterium of part in the raw material;
2. prewet: water is mixed the sheep excrement wet before building heap, will there are flowing out on every side;
3. the fermentation of banking up: will mix wet sheep excrement, pile high 1.0m-1.2m, wide 2m-3m, the trapezoidal heap that length is not limit, the control of the culture material of every heap be at dry weight 250kg-500kg, carry out heap after, to pat all around, insert some vertical pores with the wooden stick of diameter 10cm-15cm at heap again, at the bottom of the straight through reactor, stomatal frequency is 1/m
3, cover straw mat at last by its spontaneous fermentation;
4. turning: after the material temperature reaches 60 ℃, keep 24h, carry out turning, admix other auxiliary material (earlier with cotton seed hulls, earth, rapeseed cake, terra alba is mixed thoroughly) then equably under drying regime, and look the suitable moisturizing of water content of windrow, turning is 2 times altogether, time remaining 3d-4d;
When 5. stopping fermentation, the sheep faecal pellet reaches no dry granular Bai Xinwu ammonia flavor, and water content was 45% (being puffy), and the water content of cotton seed hulls mixed accessories is 65%;
6. bottling: with the sheep faecal pellet culture material 1100ml * 78mmPP culturing bottle (do not press during bottling, be state of nature and get final product) of packing into, cover the breathe freely bottle cap of sponge of band then;
7. sterilize, cool off: culture material is carried out autoclaving, keep 100 ℃ of sterilization 1h earlier, rise to 121 ℃ of autoclaving 2h then; Process of cooling is: naturally cool to below 25 ℃.
Culture material is after inserting the female kind of mushroom, and culturing room's temperature control is at 22 ℃-24 ℃, and relative air humidity is controlled at 60%-75%; Through the cultivation of 26d-28d, mycelia is covered with bottle, namely can be used as to make the cultivar use.
The present invention is raw material with the sheep faecal pellet, adds cotton seed hulls, earth, rapeseed cake as intergranular bridging material, makes the mushroom original seed.Compare with the wheat kind, its advantage is: the one, and sheep excrement comprehensive nutrition, mycelial growth is vigorous, and spawn activity is strong; The 2nd, particulate state sheep caprophyl kind, during the switching cultivar, sowing quantity is few, high efficiency; The 3rd, the sterilization link of the production of hybrid seeds has been killed intestinal bacteria, parasitic ovum and weed seed in the sheep excrement, has purified the hygienic condition of Yang Chang; The 4th, widened culture material raw material channel, reduced the bacterial classification cost of manufacture.
Embodiment
Mode 1: mushroom original seed culture material comprises following component and quality proportioning in the present embodiment: sheep faecal pellet 86%, and cotton seed hulls 4.5%, dried earth 5%, rapeseed cake 1.5%, terra alba 3% all is the quality of dry-matter, the proportioning sum is 100%.
Making method:
1. shine material: the 1d-2d that before spice raw material is exposed to the sun, utilize ultraviolet ray to kill the assorted bacterium of part in the raw material;
2. prewet: water is mixed the sheep excrement wet before building heap, will there are flowing out on every side;
3. the fermentation of banking up: will mix wet sheep excrement, pile high 1.0m, wide 2.5m, the trapezoidal heap of length 4m, carry out heap after, will around pat, insert some vertical pores with the wooden stick of diameter 10cm-15cm at heap again, at the bottom of the straight through reactor, stomatal frequency is 1/m
3, cover straw mat at last by its spontaneous fermentation;
4. turning: after the material temperature reaches 60 ℃, keep 24h, carry out turning, admix other auxiliary material (earlier with cotton seed hulls, earth, rapeseed cake, terra alba is mixed thoroughly) then equably under drying regime, and look the suitable moisturizing of water content of windrow, turning is 2 times altogether, time remaining 3d-4d;
When 5. stopping fermentation, the sheep faecal pellet reaches no dry granular Bai Xinwu ammonia flavor, and water content was 45% (being puffy), and the water content of cotton seed hulls mixed accessories is 65%;
6. bottling: with the sheep faecal pellet culture material 1100ml * 78mmPP culturing bottle (do not press during bottling, be state of nature and get final product) of packing into, cover the breathe freely bottle cap of sponge of band then;
7. sterilize, cool off: culture material is carried out autoclaving, keep 100 ℃ of sterilization 1h earlier, rise to 121 ℃ of autoclaving 2h then; Process of cooling is: naturally cool to below 25 ℃.
Culture material is after inserting the female kind of mushroom, and culturing room's temperature control is at 22 ℃, and relative air humidity is controlled 65%; Through the cultivation of 27d, mycelia is covered with bottle, namely can be used as to make the cultivar use.
Mode 2: mushroom original seed culture material comprises following component and quality proportioning in the present embodiment: sheep faecal pellet 88%, and cotton seed hulls 3.5%, dried earth 4%, rapeseed cake 1.5%, terra alba 3% all is the quality of dry-matter, the proportioning sum is 100%.Making method is with reference to mode 1.
Mode 3 (control group): mushroom kernel kind culture material: wheat 97%, terra alba 3%.
By following table as can be known, the sprout time of three kinds of mode mushroom myceliums is 36h, and mycelial growth is dense, pure white, sturdy; The full bottle of sheep faecal pellet culture material time ratio control group shortens 3d, and from digestion time, the original seed that utilizes the sheep faecal pellet to make is difficult for aging, can satisfy to prolong cultivar production to the time requirement of original seed.
Table three group culture material is to the influence of original seed mycelial growth
| Mode | Sprout time (h) | Mycelial density | Healthy and strong degree | The full bottle time (d) | Digestion time (d) |
| 1 | 36 | ++++ | Pure white, sturdy | 27 | 68 |
| 2 | 36 | ++++ | Pure white, sturdy | 27 | 68 |
| 3 | 36 | ++++ | Pure white, sturdy | 30 | 60 |
Annotate: mycelial density with ++, +++and ++ ++ expression ,+number more multilist shows that mycelial density is more high.
Claims (5)
1. mushroom original seed culture material compatibility is characterized in that containing following component and quality proportioning: sheep faecal pellet 85%-90%, and cotton seed hulls 3%-5%, dried earth 3%-5%, rapeseed cake 1.5%, terra alba 3% all is the quality of dry-matter, the proportioning sum is 100%.
2. mushroom original seed culture material according to claim 1 is characterized in that described sheep faecal pellet is the argol grain after fresh sheep excrement dries.
3. mushroom original seed culture material according to claim 1 is characterized in that described earth is the following dried loam of arable layer 18cm-20cm.
4. the making method of the mushroom original seed culture material described in the claim 1-3 is characterized in that:
1. shine material: the 1d-2d that before spice raw material is exposed to the sun, utilize ultraviolet ray to kill the assorted bacterium of part in the raw material;
2. prewet: water is mixed the sheep excrement wet before building heap, will there are flowing out on every side;
3. the fermentation of banking up: will mix wet sheep excrement, pile high 1.0m-1.2m, wide 2m-3m, the trapezoidal heap that length is not limit, the control of the culture material of every heap be at dry weight 250kg-500kg, carry out heap after, to pat all around, insert some vertical pores with the wooden stick of diameter 10cm-15cm at heap again, at the bottom of the straight through reactor, stomatal frequency is 1/m
3, cover straw mat at last by its spontaneous fermentation;
4. turning: after the material temperature reaches 60 ℃, keep 24h, carry out turning, admix other auxiliary material (earlier with cotton seed hulls, earth, rapeseed cake, terra alba is mixed thoroughly) then equably under drying regime, and look the suitable moisturizing of water content of windrow, turning is 2 times altogether, time remaining 3d-4d;
When 5. stopping fermentation, the sheep faecal pellet reaches no dry granular Bai Xinwu ammonia flavor, and water content was 45% (being puffy), and the water content of cotton seed hulls mixed accessories is 65%;
6. bottling: with the sheep faecal pellet culture material 1100ml * 78mmPP culturing bottle (do not press during bottling, be state of nature and get final product) of packing into, cover the breathe freely bottle cap of sponge of band then;
7. sterilize, cool off: culture material is carried out autoclaving, keep 100 ℃ of sterilization 1h earlier, rise to 121 ℃ of autoclaving 2h then; Process of cooling is: naturally cool to below 25 ℃.
5. utilize the making method of the mushroom original seed culture material described in the claim 1-4, after it is characterized in that culture material inserts female the kind, culturing room's temperature control is at 22 ℃-24 ℃, relative air humidity control is at 60%-75%, cultivation through 26d-28d, mycelia is covered with bottle, namely can be used as to make the cultivar use.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104823712A (en) * | 2015-04-30 | 2015-08-12 | 上海市农业科学院 | Anti-aging agaricus bisporus wheat strain and preparation method thereof |
| CN105801219A (en) * | 2016-04-27 | 2016-07-27 | 许五妮 | Method for preparing mushroom culture medium by using sheep manure |
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| CN1095885A (en) * | 1993-10-30 | 1994-12-07 | 朱斗锡 | The cultivation method of hickory chick |
| CN1370396A (en) * | 2002-04-09 | 2002-09-25 | 梁峥 | Selenium-rich mushroom and its cultivation process |
| CN1788540A (en) * | 2004-12-17 | 2006-06-21 | 中国科学院沈阳应用生态研究所 | Culture medium for Agaricus bisporus production and its application |
| CN1826859A (en) * | 2005-02-28 | 2006-09-06 | 广东省微生物研究所 | Highly efficient tier rack type straw mushroom cultivation method |
| CN101302479A (en) * | 2008-07-09 | 2008-11-12 | 赵大振 | Aromatic mushroom spawn and method for producing aromatic mushroom with the same |
| CN101496486A (en) * | 2009-03-13 | 2009-08-05 | 新疆巴音郭楞蒙古自治州农业科学研究所 | Method for cultivating mushroom |
| CN102369836A (en) * | 2010-08-18 | 2012-03-14 | 赵昌银 | Cultivation method of selenium-enriched hericium erinaceus |
-
2013
- 2013-06-18 CN CN2013102704870A patent/CN103342598A/en active Pending
Patent Citations (7)
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|---|---|---|---|---|
| CN1095885A (en) * | 1993-10-30 | 1994-12-07 | 朱斗锡 | The cultivation method of hickory chick |
| CN1370396A (en) * | 2002-04-09 | 2002-09-25 | 梁峥 | Selenium-rich mushroom and its cultivation process |
| CN1788540A (en) * | 2004-12-17 | 2006-06-21 | 中国科学院沈阳应用生态研究所 | Culture medium for Agaricus bisporus production and its application |
| CN1826859A (en) * | 2005-02-28 | 2006-09-06 | 广东省微生物研究所 | Highly efficient tier rack type straw mushroom cultivation method |
| CN101302479A (en) * | 2008-07-09 | 2008-11-12 | 赵大振 | Aromatic mushroom spawn and method for producing aromatic mushroom with the same |
| CN101496486A (en) * | 2009-03-13 | 2009-08-05 | 新疆巴音郭楞蒙古自治州农业科学研究所 | Method for cultivating mushroom |
| CN102369836A (en) * | 2010-08-18 | 2012-03-14 | 赵昌银 | Cultivation method of selenium-enriched hericium erinaceus |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104823712A (en) * | 2015-04-30 | 2015-08-12 | 上海市农业科学院 | Anti-aging agaricus bisporus wheat strain and preparation method thereof |
| CN104823712B (en) * | 2015-04-30 | 2017-03-22 | 上海市农业科学院 | Anti-aging agaricus bisporus wheat strain and preparation method thereof |
| CN105801219A (en) * | 2016-04-27 | 2016-07-27 | 许五妮 | Method for preparing mushroom culture medium by using sheep manure |
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Application publication date: 20131009 |