CN103333847A - 具有辅助降血脂功能的发酵乳杆菌grx08及其应用 - Google Patents
具有辅助降血脂功能的发酵乳杆菌grx08及其应用 Download PDFInfo
- Publication number
- CN103333847A CN103333847A CN2013103092227A CN201310309222A CN103333847A CN 103333847 A CN103333847 A CN 103333847A CN 2013103092227 A CN2013103092227 A CN 2013103092227A CN 201310309222 A CN201310309222 A CN 201310309222A CN 103333847 A CN103333847 A CN 103333847A
- Authority
- CN
- China
- Prior art keywords
- cholesterol
- grx08
- group
- bacterial strain
- lactobacillus fermenti
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000186840 Lactobacillus fermentum Species 0.000 title claims abstract description 28
- 210000004369 blood Anatomy 0.000 title claims abstract description 11
- 239000008280 blood Substances 0.000 title claims abstract description 11
- 230000009467 reduction Effects 0.000 title abstract description 3
- 230000001580 bacterial effect Effects 0.000 claims abstract description 53
- 241000894006 Bacteria Species 0.000 claims abstract description 22
- 235000015140 cultured milk Nutrition 0.000 claims description 27
- 229940012969 lactobacillus fermentum Drugs 0.000 claims description 21
- 238000000855 fermentation Methods 0.000 claims description 10
- 230000004151 fermentation Effects 0.000 claims description 10
- 238000002360 preparation method Methods 0.000 claims description 10
- 239000000203 mixture Substances 0.000 claims description 4
- 230000001105 regulatory effect Effects 0.000 claims description 3
- 238000009472 formulation Methods 0.000 claims description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 abstract description 114
- 235000012000 cholesterol Nutrition 0.000 abstract description 46
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 abstract description 18
- 238000012360 testing method Methods 0.000 abstract description 10
- 235000014655 lactic acid Nutrition 0.000 abstract description 9
- 230000000968 intestinal effect Effects 0.000 abstract description 8
- 241001465754 Metazoa Species 0.000 abstract description 7
- 239000004310 lactic acid Substances 0.000 abstract description 5
- 238000006243 chemical reaction Methods 0.000 abstract description 4
- 229940099352 cholate Drugs 0.000 abstract description 4
- BHQCQFFYRZLCQQ-OELDTZBJSA-N cholic acid Chemical compound C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 BHQCQFFYRZLCQQ-OELDTZBJSA-N 0.000 abstract description 4
- 238000000354 decomposition reaction Methods 0.000 abstract description 4
- 238000002474 experimental method Methods 0.000 abstract description 4
- 238000012216 screening Methods 0.000 abstract description 4
- 238000000338 in vitro Methods 0.000 abstract description 3
- 238000001727 in vivo Methods 0.000 abstract description 2
- 230000001737 promoting effect Effects 0.000 abstract description 2
- 102000053602 DNA Human genes 0.000 abstract 2
- 108020004414 DNA Proteins 0.000 abstract 2
- 235000011389 fruit/vegetable juice Nutrition 0.000 abstract 1
- 210000004051 gastric juice Anatomy 0.000 abstract 1
- 210000001035 gastrointestinal tract Anatomy 0.000 abstract 1
- 210000004185 liver Anatomy 0.000 description 30
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 20
- 235000013336 milk Nutrition 0.000 description 19
- 239000008267 milk Substances 0.000 description 19
- 210000004080 milk Anatomy 0.000 description 19
- 241000700159 Rattus Species 0.000 description 14
- 230000003247 decreasing effect Effects 0.000 description 14
- 230000000694 effects Effects 0.000 description 14
- 238000000034 method Methods 0.000 description 14
- 239000000843 powder Substances 0.000 description 14
- 210000000941 bile Anatomy 0.000 description 12
- 239000002689 soil Substances 0.000 description 12
- 238000002156 mixing Methods 0.000 description 11
- 239000012530 fluid Substances 0.000 description 10
- 235000013305 food Nutrition 0.000 description 9
- 239000000047 product Substances 0.000 description 9
- 239000000523 sample Substances 0.000 description 8
- 238000005070 sampling Methods 0.000 description 8
- 150000002632 lipids Chemical class 0.000 description 7
- 239000002609 medium Substances 0.000 description 7
- 108010028554 LDL Cholesterol Proteins 0.000 description 6
- 230000008859 change Effects 0.000 description 6
- 239000007788 liquid Substances 0.000 description 6
- 230000001954 sterilising effect Effects 0.000 description 6
- 238000004659 sterilization and disinfection Methods 0.000 description 6
- 230000004083 survival effect Effects 0.000 description 6
- HSINOMROUCMIEA-FGVHQWLLSA-N (2s,4r)-4-[(3r,5s,6r,7r,8s,9s,10s,13r,14s,17r)-6-ethyl-3,7-dihydroxy-10,13-dimethyl-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1h-cyclopenta[a]phenanthren-17-yl]-2-methylpentanoic acid Chemical compound C([C@@]12C)C[C@@H](O)C[C@H]1[C@@H](CC)[C@@H](O)[C@@H]1[C@@H]2CC[C@]2(C)[C@@H]([C@H](C)C[C@H](C)C(O)=O)CC[C@H]21 HSINOMROUCMIEA-FGVHQWLLSA-N 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 5
- 239000003613 bile acid Substances 0.000 description 5
- 229960000935 dehydrated alcohol Drugs 0.000 description 5
- 230000002496 gastric effect Effects 0.000 description 5
- 239000006041 probiotic Substances 0.000 description 5
- 230000000529 probiotic effect Effects 0.000 description 5
- 235000018291 probiotics Nutrition 0.000 description 5
- 210000002966 serum Anatomy 0.000 description 5
- 235000013618 yogurt Nutrition 0.000 description 5
- 102000004190 Enzymes Human genes 0.000 description 4
- 108010023302 HDL Cholesterol Proteins 0.000 description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- YBCFASPYBHNQOC-UHFFFAOYSA-N [P].[S].[Fe] Chemical compound [P].[S].[Fe] YBCFASPYBHNQOC-UHFFFAOYSA-N 0.000 description 4
- 230000004913 activation Effects 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- 238000001914 filtration Methods 0.000 description 4
- 235000015243 ice cream Nutrition 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 210000002784 stomach Anatomy 0.000 description 4
- 239000006228 supernatant Substances 0.000 description 4
- 238000005303 weighing Methods 0.000 description 4
- 108020004465 16S ribosomal RNA Proteins 0.000 description 3
- 208000024172 Cardiovascular disease Diseases 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 208000035150 Hypercholesterolemia Diseases 0.000 description 3
- 241000186660 Lactobacillus Species 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 229930006000 Sucrose Natural products 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 238000001816 cooling Methods 0.000 description 3
- 235000005911 diet Nutrition 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 3
- 230000002550 fecal effect Effects 0.000 description 3
- 238000011081 inoculation Methods 0.000 description 3
- 239000002054 inoculum Substances 0.000 description 3
- 229940039696 lactobacillus Drugs 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 230000000630 rising effect Effects 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 2
- 241000283690 Bos taurus Species 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 239000008186 active pharmaceutical agent Substances 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 210000005252 bulbus oculi Anatomy 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000005138 cryopreservation Methods 0.000 description 2
- 230000002354 daily effect Effects 0.000 description 2
- 230000037213 diet Effects 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- 230000002526 effect on cardiovascular system Effects 0.000 description 2
- 239000000839 emulsion Substances 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 210000001087 myotubule Anatomy 0.000 description 2
- 235000021590 normal diet Nutrition 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 238000012856 packing Methods 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 230000035479 physiological effects, processes and functions Effects 0.000 description 2
- 239000002504 physiological saline solution Substances 0.000 description 2
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 2
- 229920000053 polysorbate 80 Polymers 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 238000005057 refrigeration Methods 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 238000013207 serial dilution Methods 0.000 description 2
- 239000000377 silicon dioxide Substances 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000003381 stabilizer Substances 0.000 description 2
- -1 sucrose ester Chemical class 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- WRFHGDPIDHPWIQ-UHFFFAOYSA-N 2-[4-[(2-butyl-4-oxo-1,3-diazaspiro[4.4]non-1-en-3-yl)methyl]-2-(ethoxymethyl)phenyl]-n-(4,5-dimethyl-1,2-oxazol-3-yl)benzenesulfonamide Chemical compound O=C1N(CC=2C=C(COCC)C(=CC=2)C=2C(=CC=CC=2)S(=O)(=O)NC=2C(=C(C)ON=2)C)C(CCCC)=NC21CCCC2 WRFHGDPIDHPWIQ-UHFFFAOYSA-N 0.000 description 1
- IZVFFXVYBHFIHY-SKCNUYALSA-N 5alpha-cholest-7-en-3beta-ol Chemical compound C1[C@@H](O)CC[C@]2(C)[C@@H](CC[C@@]3([C@@H]([C@H](C)CCCC(C)C)CC[C@H]33)C)C3=CC[C@H]21 IZVFFXVYBHFIHY-SKCNUYALSA-N 0.000 description 1
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 201000001320 Atherosclerosis Diseases 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 1
- 235000017491 Bambusa tulda Nutrition 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 206010067125 Liver injury Diseases 0.000 description 1
- 239000005913 Maltodextrin Substances 0.000 description 1
- 229920002774 Maltodextrin Polymers 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 239000005662 Paraffin oil Substances 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 244000082204 Phyllostachys viridis Species 0.000 description 1
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 1
- 241000700157 Rattus norvegicus Species 0.000 description 1
- 208000034189 Sclerosis Diseases 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- 102000003929 Transaminases Human genes 0.000 description 1
- 108090000340 Transaminases Proteins 0.000 description 1
- QCJQWJKKTGJDCM-UHFFFAOYSA-N [P].[S] Chemical compound [P].[S] QCJQWJKKTGJDCM-UHFFFAOYSA-N 0.000 description 1
- 206010000059 abdominal discomfort Diseases 0.000 description 1
- 229960000583 acetic acid Drugs 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 229940040526 anhydrous sodium acetate Drugs 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 230000002402 anti-lipaemic effect Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000000923 atherogenic effect Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 239000011425 bamboo Substances 0.000 description 1
- 238000010241 blood sampling Methods 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000013872 defecation Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000005238 degreasing Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000000378 dietary effect Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 235000016693 dipotassium tartrate Nutrition 0.000 description 1
- 230000035622 drinking Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000012869 ethanol precipitation Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 235000013861 fat-free Nutrition 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 244000144992 flock Species 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 235000015203 fruit juice Nutrition 0.000 description 1
- 239000012362 glacial acetic acid Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 231100000753 hepatic injury Toxicity 0.000 description 1
- 230000007366 host health Effects 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Substances [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 1
- 210000005229 liver cell Anatomy 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- ZBJWWKFMHOAPNS-UHFFFAOYSA-N loretin Chemical compound C1=CN=C2C(O)=C(I)C=C(S(O)(=O)=O)C2=C1 ZBJWWKFMHOAPNS-UHFFFAOYSA-N 0.000 description 1
- 229950010248 loretin Drugs 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 229940035034 maltodextrin Drugs 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 210000004165 myocardium Anatomy 0.000 description 1
- 230000000474 nursing effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000003305 oral gavage Methods 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 239000002831 pharmacologic agent Substances 0.000 description 1
- AVTYONGGKAJVTE-OLXYHTOASA-L potassium L-tartrate Chemical compound [K+].[K+].[O-]C(=O)[C@H](O)[C@@H](O)C([O-])=O AVTYONGGKAJVTE-OLXYHTOASA-L 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000007639 printing Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 108700022737 rat Fat1 Proteins 0.000 description 1
- 238000011552 rat model Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 235000020183 skimmed milk Nutrition 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 238000012859 sterile filling Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000004114 suspension culture Methods 0.000 description 1
- 239000006188 syrup Substances 0.000 description 1
- 235000020357 syrup Nutrition 0.000 description 1
- 238000007669 thermal treatment Methods 0.000 description 1
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 description 1
- 238000002525 ultrasonication Methods 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 235000008939 whole milk Nutrition 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Images
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
Description
实验分组 | 日常饲喂 | 灌胃 |
1对照组 | 普通饲料+水 | 生理盐水 |
2高脂组 | 高脂饲料+水 | 生理盐水 |
3高脂奶粉组 | 高脂饲料+水 | 10%脱脂乳 |
4高浓度组 | 高脂饲料+水 | 悬浮108CFU/mL菌株的10%脱脂乳 |
5中浓度组 | 高脂饲料+水 | 悬浮107CFU/mL菌株的10%脱脂乳 |
6低浓度组 | 高脂饲料+水 | 悬浮106CFU/mL菌株的10%脱脂乳 |
7发酵乳组 | 高脂饲料+水 | 发酵乳 |
8细胞破碎液组 | 高脂饲料+水 | 108CFU/mL菌株的10%脱脂乳的细胞破碎液 |
组别 | 肝重/体重(g/100g) |
照组 | 3.53±0.31b |
高脂组 | 4.80±0.32a |
高脂奶粉组 | 4.35±0.37a |
高浓度组 | 3.62±0.36b |
中浓度组 | 3.77±0.33b |
低浓度组 | 3.82±0.36b |
发酵乳组 | 3.43±0.27b |
细胞破碎组 | 4.20±0.31ab |
组别 | 肝脏中胆固醇含量(mg/g) |
对照组 | 9.50±0.35a |
高脂组 | 23.16±2.95b |
高脂奶粉组 | 27.99±3.56b |
高浓度组 | 14.55±1.42c |
中浓度组 | 17.99±3.20c |
低浓度组 | 20.63±1.16b |
发酵乳组 | 14.30±2.99c |
细胞破碎组 | 23.64±4.78b |
组别 | 粪便中胆固醇含量(mg/g) | 粪便中胆汁酸的含量(mg/g) |
对照组 | 6.12±1.20a | 4.23±3.91a |
高脂组 | 12.31±2.17b | 13.59±1.32b |
高脂奶粉组 | 11.91±0.82b | 12.47±1.18b |
高浓度组 | 18.92±1.96c | 17.13±2.17c |
中浓度组 | 16.58±1.22c | 16.68±1.06c |
低浓度组 | 13.99±1.76b | 13.12±1.91b |
发酵乳组 | 17.95±1.31c | 18.49±2.09c |
细胞破碎组 | 12.10±2.01b | 12.31±0.71b |
编号 | 发酵管 | 菌株 | 编号 | 发酵管 | 菌株 |
0 | CTRL | - | 25 | ESC | + |
1 | GLY | - | 26 | SAL | + |
2 | ERY | - | 27 | CEL | + |
3 | DARA | - | 28 | MAL | + |
4 | LARA | - | 29 | LAC | + |
5 | RIB | + | 30 | MEL | - |
6 | DXYL | + | 31 | SAC | - |
7 | LXYL | - | 32 | TRE | + |
8 | ADO | - | 33 | INU | - |
9 | MDX | - | 34 | MLZ | - |
10 | GAL | - | 35 | RAF | - |
11 | GLU | + | 36 | AMD | - |
12 | FRU | + | 37 | GLYG | - |
13 | MNE | + | 38 | XLT | + |
14 | SBE | + | 39 | GEN | + |
15 | RHA | - | 40 | TUR | + |
16 | DUL | + | 41 | LYX | + |
17 | INO | - | 42 | TAG | + |
18 | MAN | - | 43 | DFUC | + |
19 | SOR | + | 44 | LFUC | - |
20 | MDM | + | 45 | DARL | + |
21 | MDG | - | 46 | LARL | - |
22 | NAG | - | 47 | GNT | - |
23 | AMY | + | 48 | 2KG | - |
24 | ARB | + | 49 | 5KG | + |
原料 | 质量百分比(%) |
发酵乳杆菌grx08 | 400kg |
白砂糖 | 75kg |
全脂奶粉 | 62.5kg |
黄奶油 | 31.25kg |
椰子油 | 23kg |
糖浆 | 26.25kg |
乳化稳定剂 | 6kg |
水 | 276kg |
Claims (4)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201310309222.7A CN103333847B (zh) | 2013-07-22 | 2013-07-22 | 具有辅助降血脂功能的发酵乳杆菌grx08及其应用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201310309222.7A CN103333847B (zh) | 2013-07-22 | 2013-07-22 | 具有辅助降血脂功能的发酵乳杆菌grx08及其应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN103333847A true CN103333847A (zh) | 2013-10-02 |
CN103333847B CN103333847B (zh) | 2014-11-26 |
Family
ID=49242052
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201310309222.7A Active CN103333847B (zh) | 2013-07-22 | 2013-07-22 | 具有辅助降血脂功能的发酵乳杆菌grx08及其应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN103333847B (zh) |
Cited By (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103937716A (zh) * | 2014-04-17 | 2014-07-23 | 扬州大学 | 人源发酵乳杆菌grx07及其应用 |
WO2016008084A1 (zh) * | 2014-07-15 | 2016-01-21 | 王国全 | 自体来源乳酸菌的制造方法及系统 |
CN105765057A (zh) * | 2013-07-22 | 2016-07-13 | Pl生物株式会社 | 从长寿村成人分离的有助于排便活动的新型乳酸菌发酵乳杆菌 |
CN109430380A (zh) * | 2018-12-10 | 2019-03-08 | 扬州大学 | 一种具有辅助降低胆固醇作用的人源混合乳酸菌发酵乳及其制备方法 |
CN109479956A (zh) * | 2018-12-10 | 2019-03-19 | 扬州大学 | 一种具有调节血脂作用的人源混合乳酸菌发酵乳及其应用 |
CN110218681A (zh) * | 2019-06-25 | 2019-09-10 | 吉林农业大学 | 一株发酵乳杆菌kp101及其应用 |
CN112602775A (zh) * | 2021-01-19 | 2021-04-06 | 扬州大学 | 一种新型火麻蛋白肽发酵乳的制备方法 |
CN112708577A (zh) * | 2020-12-31 | 2021-04-27 | 扬州大学 | 一种具有高肠道粘附力和免疫调节功能的发酵乳杆菌dali02及其应用 |
CN113749155A (zh) * | 2021-08-30 | 2021-12-07 | 扬州大学 | 一种高活性发酵乳杆菌grx08发酵液的制备方法及其应用 |
CN116195743A (zh) * | 2023-03-26 | 2023-06-02 | 扬州大学 | 发酵乳杆菌grx08在制备具有缓解肥胖及糖脂代谢紊乱功能食品与药品中的应用 |
CN116268422A (zh) * | 2023-03-29 | 2023-06-23 | 扬州大学 | 发酵乳杆菌grx08在制备具有缓解酒精性肝损伤及其相关症状作用食品、功能食品与药品中应用 |
-
2013
- 2013-07-22 CN CN201310309222.7A patent/CN103333847B/zh active Active
Non-Patent Citations (3)
Title |
---|
DORA I. A. PEREIRA AND GLENN R. GIBSON: "Cholesterol Assimilation by Lactic Acid Bacteria and Bifidobacteria Isolated from the Human Gut", 《APPLIED AND ENVIRONMENTAL MICROBIOLOGY》 * |
NING XIE ET AL.: "Effects of two Lactobacillus strains on lipid metabolism and intestinal microflora in rats fed a high-cholesterol diet", 《BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE》 * |
王正根: "两株乳杆菌体外降胆固醇的筛选及相关机制的研究", 《中国博士学位论文全文数据库 医药卫生科技辑》 * |
Cited By (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105765057A (zh) * | 2013-07-22 | 2016-07-13 | Pl生物株式会社 | 从长寿村成人分离的有助于排便活动的新型乳酸菌发酵乳杆菌 |
CN103937716A (zh) * | 2014-04-17 | 2014-07-23 | 扬州大学 | 人源发酵乳杆菌grx07及其应用 |
WO2016008084A1 (zh) * | 2014-07-15 | 2016-01-21 | 王国全 | 自体来源乳酸菌的制造方法及系统 |
CN109430380A (zh) * | 2018-12-10 | 2019-03-08 | 扬州大学 | 一种具有辅助降低胆固醇作用的人源混合乳酸菌发酵乳及其制备方法 |
CN109479956A (zh) * | 2018-12-10 | 2019-03-19 | 扬州大学 | 一种具有调节血脂作用的人源混合乳酸菌发酵乳及其应用 |
CN110218681A (zh) * | 2019-06-25 | 2019-09-10 | 吉林农业大学 | 一株发酵乳杆菌kp101及其应用 |
CN110218681B (zh) * | 2019-06-25 | 2021-09-28 | 吉林农业大学 | 一株发酵乳杆菌kp101及其应用 |
CN112708577B (zh) * | 2020-12-31 | 2022-04-05 | 扬州大学 | 一种具有高肠道粘附力和免疫调节功能的发酵乳杆菌dali02及其应用 |
CN112708577A (zh) * | 2020-12-31 | 2021-04-27 | 扬州大学 | 一种具有高肠道粘附力和免疫调节功能的发酵乳杆菌dali02及其应用 |
CN112602775A (zh) * | 2021-01-19 | 2021-04-06 | 扬州大学 | 一种新型火麻蛋白肽发酵乳的制备方法 |
CN113749155A (zh) * | 2021-08-30 | 2021-12-07 | 扬州大学 | 一种高活性发酵乳杆菌grx08发酵液的制备方法及其应用 |
CN116195743A (zh) * | 2023-03-26 | 2023-06-02 | 扬州大学 | 发酵乳杆菌grx08在制备具有缓解肥胖及糖脂代谢紊乱功能食品与药品中的应用 |
CN116268422A (zh) * | 2023-03-29 | 2023-06-23 | 扬州大学 | 发酵乳杆菌grx08在制备具有缓解酒精性肝损伤及其相关症状作用食品、功能食品与药品中应用 |
Also Published As
Publication number | Publication date |
---|---|
CN103333847B (zh) | 2014-11-26 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN103333847B (zh) | 具有辅助降血脂功能的发酵乳杆菌grx08及其应用 | |
CN107502575B (zh) | 一株具有α-葡萄糖苷酶高抑制活性的植物乳杆菌 | |
CN101328470B (zh) | 具有降胆固醇及抑菌能力的鼠李糖乳杆菌grx10及其用途 | |
CN101260377B (zh) | 一种动物双歧杆菌及其用途 | |
CN100484964C (zh) | 银耳杂多糖或其提取物的新用途 | |
CN102970997B (zh) | 抗肥胖剂、抗肥胖用饮食品、糖耐量改善剂和糖耐量改善用饮食品 | |
CN101328469B (zh) | 具有酒精性肝损伤保护功能的嗜热链球菌grx02及其用途 | |
CN101649303B (zh) | 一种双歧杆菌及其应用和含有双歧杆菌的食品组合物 | |
CN103131647B (zh) | 婴儿双歧杆菌及其制剂 | |
CN110106119B (zh) | 一株分离自母乳的鼠李糖乳杆菌m9及其应用 | |
CN1568365A (zh) | 包含戊糖乳杆菌菌株的组合物及其用途 | |
CN103898017B (zh) | 源于广西巴马长寿村的鼠李糖乳杆菌在辅助降血脂方面的应用 | |
CN105815641A (zh) | 一种具有抗肿瘤活性的综合酵素液的制备方法及其用途 | |
CN116814464B (zh) | 一株发酵粘液乳杆菌jf5及其在制备减脂和助消化食品药品中的应用 | |
CN107475162A (zh) | 具有二肽基肽酶‑iv高抑制活性的鼠李糖乳杆菌及其应用 | |
CN106604736A (zh) | 副干酪乳杆菌促进生态失调后肠道菌群多样性恢复的应用 | |
CN103446552B (zh) | 用于预防和治疗消化系统疾病的发酵组合物 | |
CN106659747A (zh) | 鼠李糖乳杆菌促进生态失调后肠道菌群多样性恢复的应用 | |
CN113430148A (zh) | 一种动物双歧杆菌的发酵培养基及其应用 | |
CN108795823B (zh) | 一种改善女性孕产妇肠道菌群的益生菌的培养方法和应用 | |
CN115895966B (zh) | 一株辅助缓解痛风的两歧双歧杆菌bl002及其应用 | |
CN109576165A (zh) | 一种贝酵母菌及其应用 | |
CN113789290B (zh) | 一种具有抗疲劳作用的凝结芽孢杆菌bcn019及其菌剂和应用 | |
JP6710429B1 (ja) | 菌群 | |
WO2021020271A1 (ja) | 短鎖脂肪酸を含有する発酵液の製造方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right |
Effective date of registration: 20190703 Address after: No. 168 Tingyi Road, Tinglin Town, Jinshan District, Shanghai, 201540 Patentee after: SHANGHAI HOWYOU INDUSTRY Co.,Ltd. Address before: No. 88, South Road, University of Yangzhou, Jiangsu, Jiangsu Patentee before: Yangzhou University |
|
TR01 | Transfer of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Lactobacillus fermentans grx08 with auxiliary hypolipidemic function and its application Effective date of registration: 20220225 Granted publication date: 20141126 Pledgee: Shanghai Rural Commercial Bank Co.,Ltd. Jinshan sub branch Pledgor: SHANGHAI HOWYOU INDUSTRY Co.,Ltd. Registration number: Y2022310000037 |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
CP03 | Change of name, title or address |
Address after: Building 7, No. 168, Tingyi Road, Tinglin Town, Jinshan District, Shanghai, 201600 Patentee after: Shanghai Haoyue Biotechnology Co.,Ltd. Address before: No. 168 Tingyi Road, Tinglin Town, Jinshan District, Shanghai, 201540 Patentee before: SHANGHAI HOWYOU INDUSTRY Co.,Ltd. |
|
CP03 | Change of name, title or address | ||
PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20231107 Granted publication date: 20141126 Pledgee: Shanghai Rural Commercial Bank Co.,Ltd. Jinshan sub branch Pledgor: SHANGHAI HOWYOU INDUSTRY Co.,Ltd. Registration number: Y2022310000037 |
|
PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Fermented Lactobacillus grx08 with auxiliary lipid-lowering function and its application Granted publication date: 20141126 Pledgee: Shanghai Rural Commercial Bank Co.,Ltd. Jinshan sub branch Pledgor: Shanghai Haoyue Biotechnology Co.,Ltd. Registration number: Y2024310000039 |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right |