CN103330096B - Traditional Chinese medicine feed supplement and application thereof - Google Patents
Traditional Chinese medicine feed supplement and application thereof Download PDFInfo
- Publication number
- CN103330096B CN103330096B CN201310302628.2A CN201310302628A CN103330096B CN 103330096 B CN103330096 B CN 103330096B CN 201310302628 A CN201310302628 A CN 201310302628A CN 103330096 B CN103330096 B CN 103330096B
- Authority
- CN
- China
- Prior art keywords
- egg
- chinese herbal
- feed additive
- herbal feed
- mass parts
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
The invention discloses a traditional Chinese medicine feed supplement and an application thereof. The traditional Chinese medicine feed supplement, which is livestock and poultry feed supplement, comprises the following traditional Chinese medicine raw materials by mass part: 10-60 parts of blueberry, 10-60 parts of Indian buead, 10-60 parts of marine algae, 10-60 parts of honeysuckle and 310-60 parts of oyster. The livestock and poultry feed supplement can reduce the egg cholesterol level, improve the content of selenium, iron and zinc of the egg, as well as the DHA content of the egg and weight of spleen and bursa of fabricius, enhance the relative number of T and B lymphocyte in tonsillas in the thymus, spleen, bursa of fabricius and caecal, and improve the T lymphocyte conversion rate of blood serum of the egg.
Description
Technical field
The present invention relates to a kind of additive for farm animal feed, particularly a kind of Chinese herbal feed additive and application thereof.
Background technology
Quick and the intensivization development of modern poultry husbandry, chicken mass-sending disease is also day by day frequent, affects egg quality.Use a large amount of antibiotic, cause the medicament residue of egg to increase.Apply Chinese herb feed additive, meet the use standard of nuisanceless medicine, can not only prevent disease, and contain a large amount of nutriments, chicken group nutrition and Egg Quality are improved greatly.
DHA(Docosahexaenoic acid, i.e. DHA) be the long-chain polyunsaturated fatty acid (ω-3PUFAs) of a kind of ω of belonging to-3 series.It is one of chief component material of human brain, accounts for 10% left and right of people's cephalopin.DHA can not be synthesized in human body, only absorbs and supplements from the external world, and marine fishes, Mycophyta and seaweeds etc. are its main sources.Large quantity research shows, DHA has brain nourishing and eyesight improving, angiocardiopathy preventing, anticancer, anti-inflammatory and suppresses the function such as irritated, is the important nutrient that maintains human health.If can improve DHA content in egg, there is important function for maintaining and improving human health
Cholesterol can be divided into HDL-C (HDL-cholesterol) and LDL-C (LDL-cholesterol) according to its function.HDL-cholesterol has cleaning artery impurity, prevents arteriosclerotic effect, to human body favourable " good cholesterol "; LDL-cholesterol is the important evocator that causes disease.But the very important high nutritive peculiarity of traditional eating habit and egg is doomed its status in people's diet and is difficult to shake, how eliminating the edible taboo of egg becomes the key of egg healthy edible.For this reason, reducing cholesterol level in egg, improving cholesterol composition is the effectively section of having addressing the above problem.
Functional Egg refer to except nutritious delicious, regulate in addition the egg of human physiological activity's function.In recent years, common functional Egg has both at home and abroad:
1 iodine-enriched egg
Iodine is as the one trace element of needed by human, plays an important role maintaining in thyroid gland normal function, glucose and lipid metabolism.Large quantity research report, nutritious iodine-enriched egg is the health food that prevents iodine deficiency disease, and treatment hyperthyroidism, thyroid gland enlargement, coronary heart disease etc. are had to good result for the treatment of.Kind (1993) for a long time report on mountain, add 2% Thallus Laminariae (Thallus Eckloniae) extract (containing iodine 0.38%) in laying hen material time, in every 100 grams of yolk, content of iodine increases by 2.1～5.8mg; In the time adding 4%, in yolk, the content of iodine is about 2 times while adding 2%; In the time adding 6%, the content of iodine is about 2.5 times of interpolation 2%.Improve addition, in yolk, the content of iodine just can not be significantly increased again, and substantially keeps above-mentioned level.
2 high zinc eggs
Recent study report, the egg of high content zinc to prevention and treatment due to the disease that lacks zinc and cause, as child and Juvenile development obstacle, wound healing slowly, anaemia etc. has good clinical effectiveness.Dai Lan etc. (1994) test shows, uses certain density zinc solution to mix and stir in feed, and the 15d that feeds can make test group egg zinc content improve 67.7% than the egg zinc content of control group.Xu Jianxiong etc. (1994) report adds 1000,2000,15d Laying performance improves respectively 34.22%, 46.9% and 75.71% after 3000mg/kg zinc in feed.
The high lecithin egg of 3 low cholesterol
Research report, cellulose can adsorb cholate in enteron aisle, shortens cholesterol by the time of enteron aisle, thereby reduces the absorption of cholesterol.Rybina (1985) is increased to 15% by clover in feed, and result Cholesterol Content in Egg Yolk is only original 66.7%～50%.Wang Yucai etc. (1992) mix 2% fiber when additive is fed laying hen with chelating agent, reduced very significantly cholesterol level.
4 green eggs
Along with China's entry into the World Trade Organization, the development and production of pollution-free food more and more receives people's concern.Green egg is one of pollution-free food type, pollution-free, the noresidue that referring to that poultry produces, to the harmless egg of health.
Summary of the invention
The object of this invention is to provide a kind of Chinese herbal feed additive and application thereof.
Chinese herbal feed additive provided by the invention, its active component is made up of the Chinese medicine material of following mass parts: blueberry 10-60 mass parts, Poria cocos 10-60 mass parts, marine alga 10-60 mass parts, honeysuckle 10-60 mass parts and oyster 10-60 mass parts.
The active component of described Chinese herbal feed additive specifically can be made up of the Chinese medicine material of following mass parts: blueberry 40 mass parts, Poria cocos 40 mass parts, marine alga 40 mass parts, honeysuckle 40 mass parts and oyster 40 mass parts.
Described mass parts is dry weight.
In above-mentioned Chinese medicine material, described blueberry is the ripening fruits of Ericaceae Vaccinium plant blueberry; Described Poria cocos is On Polyporaceae Poria cocos Poria cocos(Schw.) dry sclerotia of Wolf; Described marine alga is the dwell herb of dish or Sargassum of Sargassaceae plant sheep; Described honeysuckle is the dry flower of caprifoliaceae plant honeysuckle Lonicera japonica Thunb.; Described oyster is the shell of the long oyster Ostrea of Ostreidae animal gigas Thunberg, ostrea talienwhanensis Crosse Ostrea talienwhanensis Crosse or Crassostrea rivularis Ostrea rivularis Gould.
Described Chinese medicine material can be Chinese medicine, also can be the prepared slices of Chinese crude drugs.
Described Chinese herbal feed additive can only be made up of active component, also can add other animal and fowl fodder component again.
Above-mentioned Chinese herbal feed additive is also for preparing according to following method provided by the invention the product obtaining.
The method of the described Chinese herbal feed additive of preparation provided by the invention, comprises the steps:
Described each Chinese medicine material is mixed according to described mass parts, obtain described Chinese herbal feed additive;
Or, after being mixed according to described mass parts, pulverizes again described each Chinese medicine material, obtain described Chinese herbal feed additive.
The livestock and poultry diet that contains above-mentioned any Chinese herbal feed additive and described Chinese herbal feed additive, in the application as in additive for farm animal feed, also belong to protection scope of the present invention.
Described fowl is specially chicken;
Described livestock and poultry diet is daily grain of chicken;
Described daily grain of chicken can be used as laying cycle of laying hens daily ration, also can be used as the daily ration in other period.
The mass content of additive for farm animal feed described in described daily grain of chicken can be 9%, 9%-10%, 7%-10% or 7%-9%.
The application of above-mentioned daily grain of chicken in production functional Egg also belongs to protection scope of the present invention.
Described functional Egg has at least one characteristic in a1-a5;
Wherein, described a1 is that total cholesterol level in described functional Egg is lower than the total cholesterol level in common egg;
Described a2 is that Se content in described functional Egg is higher than the Se content in described common egg;
Described a3 is that iron content in described functional Egg is higher than the iron content in described common egg;
Described a4 is that zinc content in described functional Egg is higher than the zinc content in described common egg;
Described a5 is that the content of DHA in described functional Egg is higher than the DHA content in described common egg;
Described common egg is the egg of producing according to method P:
Described method P is except the described daily grain of chicken that contains in the method for the described functional Egg of production is replaced with the common daily ration of laying cycle of laying hens, and other is all identical with the method for producing described functional Egg;
Wherein, the common daily ration of described laying cycle of laying hens is that the arbitrary described Chinese herbal feed additive in described daily grain of chicken is removed to the daily ration obtaining.
Wherein, for the production of the quality percentage composition with additive for farm animal feed described in the described daily grain of chicken of described a1 characteristic can be 9%, 9%-10%, 7%-10% or 7%-9%;
For the production of the quality percentage composition with additive for farm animal feed described in the described daily grain of chicken of described a2 characteristic can be 9%, 9%-10%, 7%-10% or 7%-9%;
For the production of the quality percentage composition with additive for farm animal feed described in the described daily grain of chicken of described a3 characteristic can be 9%, 9%-10%, 7%-10% or 7%-9%;
For the production of the quality percentage composition with additive for farm animal feed described in the described daily grain of chicken of described a4 characteristic can be 9%, 9%-10%, 7%-10% or 7%-9%;
For the production of the quality percentage composition with additive for farm animal feed described in the described daily grain of chicken of described a4 characteristic can be 9%, 9%-10%, 7%-10% or 7%-9%;
For the production of the quality percentage composition with additive for farm animal feed described in the described daily grain of chicken of described a5 characteristic can be 9%, 9%-10%, 7%-10% or 7%-9%.
The common daily ration of described laying cycle of laying hens is except not containing described additive for farm animal feed, and other raw material all can be identical with routine laying period daily ration, as contain energy feed, protein feeds, mineral feed and vitamin feed; Further, described common laying period,, feed also can contain antioxidant.The common daily ration of described laying cycle of laying hens can be made up of energy feed, protein feeds, mineral feed and vitamin feed, also can be formed by energy feed, protein feeds, mineral feed, vitamin feed and antioxidant, certainly, can also in described common laying period feed, add again the non-nutritive additives such as anticorrisive agent, colouring agent, flavor enhancement, medicinal health-care agent, growth promoter.
Wherein, described energy feed can be conventional chicken energy feed, as being selected from least one in following substances: Gu Shi, bran, potato, grass-seed and tree reality etc.
Described protein feeds can be conventional egg white matter feed, as being selected from least one in following substances: Soybean Meal, Cottonseed Meal, rapeseed dregs, fish meal, rapeseed dregs, peanut meal and the certain herbaceous plants with big flowers dregs of rice etc.
Described mineral feed can be selected from stone flour, calcium bicarbonate, salt, oyster shell whiting, bone meal, calcium monohydrogen phosphate, calcium carbonate, salt, copper sulphate, ferrous sulfate, manganese sulfate, KI, zinc oxide, sodium selenite and cobalt chloride etc.
The present invention also provides a kind of method of producing functional Egg.
The method of production functional Egg provided by the present invention, comprises the step that obtains described functional Egg to the above-mentioned daily grain of chicken of feeding in the laying hen of laying period.
It is described that to give in the laying hen of the laying period above-mentioned daily grain of chicken of feeding be to the above-mentioned daily grain of chicken 14 days above (as 14-21 days, 42 days, 14 days-42 days, 14-35 days) of feeding in the laying hen of laying period (age in 21-72 week, as 21-24 age in week, 30-37 age in week, 25-42 age in week, 43-50 age in week).
The egg of being produced by said method also belongs to protection scope of the present invention, and described egg is as propagating materials, as only as food and/or health products.
The present invention also protects described additive for farm animal feed and described livestock and poultry diet to improve the application in following at least one biochemical indicator product in chicken serum in preparation:
1) weight of raising chicken spleen;
2) weight of raising chicken bursa;
3) relative number of T lymphocyte and/or bone-marrow-derived lymphocyte in raising Immune Organs of Chichen;
4) relative number of T lymphocyte and/or bone-marrow-derived lymphocyte in raising chicken spleen;
5) relative number of T lymphocyte and/or bone-marrow-derived lymphocyte in raising chicken bursa;
6) relative number of T lymphocyte and/or bone-marrow-derived lymphocyte in raising cecal tonsil;
7) improve the lymphocytic conversion ratio of T in chicken serum.
Wherein, 1), 2) 3) 4) 5) 6) and 7) the quality percentage composition of additive for farm animal feed described in daily grain of chicken used can be 9%, 10%, 7%, 9%-10%, 7%-10% or 7%-9%;
Described product can be chicken feed.
Additive for farm animal feed of the present invention can reduce egg inner cholesterol content; The 2nd, can improve selenium, iron and the zinc content of egg; The 3rd, can improve the content of DHA in egg; The 4th, can improve the weight of spleen and the bursa of farbricius; The 5th, can strengthen the relative number of T, bone-marrow-derived lymphocyte in thymus gland, spleen, the bursa of farbricius and cecal tonsil; The 6th, improve T lymphocyte transformation rate in chicken serum.Additive for farm animal feed of the present invention, because adopting the Chinese medicine of integration of drinking and medicinal herbs, therefore to livestock and poultry animal and the mankind without any side effect.Develop green livestock and poultry industry, meet people's food security, improve nutritive value of food, strengthen the competitiveness of livestock products in international market, there is important economic implications and social benefit.
Detailed description of the invention
Following embodiment is convenient to understand better the present invention, but does not limit the present invention.Experimental technique in following embodiment, if no special instructions, is conventional method.Material, reagent etc. used in following embodiment, if no special instructions, all can obtain from commercial channels.
In the Chinese medicine material of following embodiment, blueberry is the ripening fruits of Ericaceae Vaccinium plant blueberry, Poria cocos is On Polyporaceae Poria cocos Poria cocos(Schw.) dry sclerotia of Wolf, marine alga is the dwell herb of dish or Sargassum of Sargassaceae plant sheep, honeysuckle is the dry flower of caprifoliaceae plant honeysuckle Lonicera japonica Thunb., and oyster is the shell of the long oyster Ostrea of Ostreidae animal gigas Thunberg, ostrea talienwhanensis Crosse Ostrea talienwhanensis Crosse or Crassostrea rivularis Ostrea rivularis Gould.
Embodiment 1, DHA content is high, total cholesterol level is low, the production method of the functional Egg of rich selenium, iron and zinc
One, prepare Chinese herbal feed additive
After the blueberry of integration of drinking and medicinal herbs, Poria cocos, marine alga, honeysuckle and oyster are pulverized respectively, proportioning according to blueberry 40 mass parts, Poria cocos 40 mass parts, marine alga 40 mass parts, honeysuckle 40 mass parts and oyster 40 mass parts is mixed, and obtains Chinese herbal feed additive.
Two, production functional Egg
1, laying period daily ration
Laying period,, daily ration was divided into following 4 kinds:
1.1 laying period common daily ration
Its composition is as shown in table 1.
The common daily ration composition of table 1, laying period and trophic level
| Component | Unit (gram) | ? | Trophic level |
| Corn | 63.00 | Metabolizable energy/Jkg-1 | 10852.73 |
| Dregs of beans | 24.00 | Thick protein/% | 17.6 |
| Stone flour | 8.00 | Coarse ash % | 12.94 |
| Premix | 5.00 | Crude fibre/% | 5.8 |
| ? | ? | Calcium/% | 4 |
| ? | ? | Total phosphorus/% | 1 |
| ? | ? | Available phosphorus/% | 0.62 |
| ? | ? | NaCl | 0.5 |
| ? | ? | Lysine | 1.50 |
| ? | ? | Methionine | 0.79 |
| ? | ? | Methionine+cystine | 0.80 |
| ? | ? | Threonine | 1.00 |
Wherein, in every kilogram of premix, contain vitamin A 550000U, vitamin D
3162000U, vitamin E
1100U, phytomenadione 51mg, pantothenic acid 410mg, folic acid 45mg, biotin 4.5mg, Choline Chloride 20g, Cobastab
1120mg, Cobastab
2295mg, Cobastab
666mg, Cobastab
120.25mg; Trace element contains Fe4g, Zn4.1g, Mn4.9g, Cu0.4g, I75mg, Se18mg.
Wherein, vitamin A is in vitamin(e) A acetate, 1IU vitamin A=0.344 μ g vitamin(e) A acetate;
Vitamin D
3in Vitamin D3,1IU vitamin D
3=0.025 μ g Vitamin D3;
Vitamin E
1in alpha-tocopherol, 1IU vitamin E
1=0.668mg alpha-tocopherol.
1.27% Chinese medicine daily ration.
Laying period 1.1 is added step 1 gained Chinese herbal feed additive in common daily ration, addition be this laying period common daily ration quality 7%, obtain 7% Chinese medicine daily ration.
1.39% Chinese medicine daily ration.
Laying period 1.1 is added step 1 gained Chinese herbal feed additive in common daily ration, addition be this laying period common daily ration quality 9%, obtain 9% Chinese medicine daily ration.
1.410% Chinese medicine daily ration.
Laying period 1.1 is added step 1 gained Chinese herbal feed additive in common daily ration, addition be this laying period common daily ration quality 10%, obtain 10% Chinese medicine daily ration.
2, experimental animal
Blue grey laying hen 96 plumages in health sea that age in days, weight, laying period and laying rate are close are selected in test, be divided at random 4 processed group: common daily ration group of laying period (abbreviation control group), 7% Chinese medicine daily ration group, 9% Chinese medicine daily ration group, 10% Chinese medicine daily ration group, every group of 6 repetitions, 4 chickens of each repetition.
3, test method
By above-mentioned 4 processed group respectively with corresponding laying period daily ration raise in advance 7 days, formally feed 28 days, test chicken 8:00 every day and 18:00 feeding, free choice feeding, nipple-shape water dispenser is freely drunk water, illumination every day 16h, keeps air quality in house at gravity-flow ventilation.In starting to get at random 6 pieces, egg sample each repetition in the 14th day, 24 days, 28 days from raising in advance, after sampling, egg is carried out to mark and put into 4 DEG C of refrigerators and preserve in experimental period.
Wherein, common daily ration group of laying period (abbreviation control group) with above-mentioned laying period common daily ration feed, 7% Chinese medicine daily ration group is fed with above-mentioned 7% Chinese medicine daily ration, and 9% Chinese medicine daily ration group is fed with above-mentioned 9% Chinese medicine daily ration, and 10% Chinese medicine daily ration group is fed with above-mentioned 10% Chinese medicine daily ration.Other raising condition is all identical.
Wherein, in whole experimentation, laying hen is carried out conventional immune programme for children, the stress reaction that laying hen causes without disease, environment.
4, testing index and method
4.1 performance test
Duration of test, records laying rate, egg quality and the feed intake of each group of chicken every day, calculates average egg quality, laying rate and feedstuff-egg ratio, and formula is as follows:
Average egg weight=every group of total egg quality/every group of number of laying eggs,
Laying rate=number/livestock on hand chicken number × 100% of laying eggs,
Feedstuff-egg ratio=feed consumption rate/total nitrogen quality
The mensuration of 4.2 Egg Qualities
Measure egg size, egg shape index, shell thickness, eggshell weight, hangh unit, yolk color: the Zhou Gui of every piece of egg sample according to the method for following document, Wang Like, Huang Ruihua etc.Production of Livestock and Poultry experiment study course. China Agricultyre University Press .2006.
The mensuration of 4.3 egg nutritional labelings
The yolk of egg sample and albumen are stirred evenly, smashed, in 65 DEG C of baking ovens, dry 5～6h, after taking-up, in room air, cooling 4h, weighs, and makes air-dry sample.Measure thick protein, phosphatide, vitamin A, selenium, iron, zinc, vitamin E: GB5009.5-2010, GB/T5009.128-2003, GB/T5009.82-2003 according to the method in following standard.
Egg sample is boiled, get yolk and measure cholesterol level: Zhou Gui according to the method for following document, Wang Like,, Huang Ruihua etc.Production of Livestock and Poultry experiment study course. China Agricultyre University Press, 2006.
DHA: with reference to (Li Jings such as Li Jings, Deng Zeyuan, topaz China. the mensuration [J] of Fatty Acids of Egg. University Of Nanchang's journal: natural sciences version, 2007,31 (2): 164-16) method is measured DHA content in egg, the mensuration of this experimental data is tested laboratory, Co., Ltd Beijing and is completed by composing Buddhist nun.
EPA: measure EPA content in egg with reference to GB/T541327-2007, the mensuration of this experimental data is tested laboratory, Co., Ltd Beijing and completed by composing Buddhist nun.
5, data processing
Test data adopts SAS software to carry out variance analysis, and Duncan ' s method is carried out multiple ratio, and result represents with " mean+SD ".
6, results and analysis
The impact of 6.1 Chinese herbal feed additives on Egg Quality
Chinese herbal feed additive on the impact of Egg Quality in table 2.
The egg quality of table 2, each group
| Project | Control group | 7% Chinese medicine daily ration group | 9% Chinese medicine daily ration group | 10% Chinese medicine daily ration group |
| Average egg weight/g | 64.72±0.33 b | 64.97±1.83 b | 69.46±0.47 a | 67.89±1.37 b |
| Laying rate/% | 84.32±1.32 b | 86.50±1.50 a | 88.36±1.05 b | 87.72±1.24 a |
| Feedstuff-egg ratio | 2.33±0.93 b | 2.31±0.86 ab | 2.22±0.58 a | 2.28±0.74 ab |
| Hangh unit | 78.45±4.71 a | 79.57±4.70 a | 79.59±3.72 a | 79.69±6.43 a |
| Shell thickness/mm | 0.41±0.03 a | 0.43±0.076 a | 0.43±0.04 a | 0.42±0.03 a |
| Eggshell weight/g | 7.51±0.14 a | 7.42±0.27 a | 7.37±0.13 a | 7.38±0.31 a |
| Yolk color | 7.44±0.48 b | 7.57±0.75 b | 8.57±0.37 a | 8.41±0.89 ab |
| Egg shape index | 0.75±0.06 a | 0.76±0.02 a | 0.77±0.04 a | 0.76±0.08 a |
As shown in Table 2, along with the increase of Chinese herbal feed additive addition, egg size and laying rate increase gradually, the 9% Chinese medicine daily ration group average egg weight utmost point is significantly higher than other groups (P<0.01), feedstuff-egg ratio is lower than other processed group, and other respectively process not remarkable (P>0.05) of group difference; .Along with the increase yolk color of addition is deepened, 9% Chinese medicine daily ration group color is the darkest, significant difference compared with control group (P<0.05), between other each processed group and control group without significant difference (P>0.05).Different interpolation levels significantly affects egg shape index, hangh unit, shell thickness, eggshell weight, nothing, not remarkable (P>0.05) of difference between each group.
The impact of 6.2 Chinese herbal feed additives on part nutrient composition content in egg
Chinese herbal feed additive on the impact of part nutrient composition content in egg in table 3.
Table 3, the impact of Chinese herbal feed additive on part nutrient composition content in egg
From table 3, the content of each test group amino acid and phosphatide and control group are all without significant difference (P>0.05).Compared with control group, the total cholesterol level in 7% Chinese medicine daily ration group, 9% Chinese medicine daily ration group and 10% Chinese medicine daily ration group egg significantly decreases, and wherein 9% Chinese medicine daily ration group total cholesterol level declines 69.70% with respect to control group.Compared with control group, selenium, iron, zinc content in 7% Chinese medicine daily ration group, 9% Chinese medicine daily ration group and 10% Chinese medicine daily ration group egg obviously raise.
DHA(Docosahexaenoic acid, i.e. DHA) be the long-chain polyunsaturated fatty acid (ω-3PUFAs) of a kind of ω of belonging to-3 series.It is one of chief component material of human brain, accounts for 10% left and right of people's cephalopin.DHA can not be synthesized in human body, only absorbs and supplements from the external world, and marine fishes, Mycophyta and seaweeds etc. are its main sources.Large quantity research shows, DHA has brain nourishing and eyesight improving, angiocardiopathy preventing, anticancer, anti-inflammatory and suppresses the function such as irritated, is the important nutrient that maintains human health.
As shown in Table 3, compared with control group, the DHA content in 7% Chinese medicine daily ration group, 9% Chinese medicine daily ration group and 10% Chinese medicine daily ration group egg obviously raises (P < 0.05), but the EPA content of egg the inside is not affected.
Embodiment 2, the impact of Chinese herbal feed additive on Laying-hen Serum biochemical indicator and immunity
1, daily ration
Daily ration is divided into following 4 kinds:
1.1 common daily rations
Its composition is as shown in table 4.
Table 4, common daily ration composition and trophic level
| Component | Unit (gram) | ? | Trophic level |
| Corn | 59.1 | Metabolizable energy/MJkg-1 | 11.71 |
| Dregs of beans | 25 | Thick protein/% | 15.9 |
| Wheat bran | 5 | Calcium/% | 3.49 |
| Stone flour | 9 | Available phosphorus/% | 0.60 |
| Calcium monohydrogen phosphate | 1.4 | Methionine/% | 0.31 |
| Salt | 0.39 | ? | ? |
| Methionine | 0.13 | ? | ? |
| B B-complex * | 0.02 | ? | ? |
| Trace element * * | 0.2 | ? | ? |
Wherein, every kilogram of B B-complex contains vitamin A 550000U, vitamin D
3162000U, vitamin E
1100U, phytomenadione 51mg, pantothenic acid 410mg, folic acid 45mg, biotin 4.5mg, Choline Chloride 20g, Cobastab
1120mg, Cobastab
2295mg, Cobastab
666mg, Cobastab
120.25mg, all the other are stone flour.Every kilogram of trace element contains Fe4g, Zn4.1g, and Mn4.9g, Cu0.4g, I75mg, Se18mg, all the other are stone flour.
1.27% Chinese medicine daily ration.
The Chinese herbal feed additive that adds 7% the embodiment 1 that is this common daily ration quality in 1.1 common daily ration obtains 7% Chinese medicine daily ration.In 7% Chinese medicine daily ration, the quality percentage composition of Chinese herbal feed additive is 7%.
1.39% Chinese medicine daily ration.
The Chinese herbal feed additive that adds 10% the embodiment 1 that is this common daily ration quality in 1.1 common daily ration obtains 9% Chinese medicine daily ration.In 9% Chinese medicine daily ration, the quality percentage composition of Chinese herbal feed additive is 9%.
1.410% Chinese medicine daily ration.
The Chinese herbal feed additive that adds 10% the embodiment 1 that is this common daily ration quality in 1.1 common daily ration obtains 10% Chinese medicine daily ration.In 10% Chinese medicine daily ration, the quality percentage composition of Chinese herbal feed additive is 10%.
2, experimental animal
Test selects same hen house with 320 of 14 age in days Luo Man laying hens of a collection of health, adopt single-factor RANDOMIZED BLOCK DESIGN, laying hen is divided into 4 processed group at random: common daily ration group (abbreviation control group), 7% Chinese medicine daily ration group, 9% Chinese medicine daily ration group, 10% Chinese medicine daily ration group, 5 repetitions of each processing, 16 chickens of each repetition.
3, test method
Above-mentioned 4 processed group are raised 7 days in advance with corresponding daily ration respectively, formally feed 60 days, test chicken 8:00 every day and 18:00 feeding, free choice feeding, nipple-shape water dispenser is freely drunk water, illumination every day 16h, air quality in gravity-flow ventilation, maintenance house.
Wherein, common daily ration group (abbreviation control group) is fed with above-mentioned common daily ration, 7% Chinese medicine daily ration group is fed with above-mentioned 7% Chinese medicine daily ration, and 9% Chinese medicine daily ration group is fed with above-mentioned 9% Chinese medicine daily ration, and 10% Chinese medicine daily ration group is fed with above-mentioned 10% Chinese medicine daily ration.Other raising condition is all identical.
Wherein, in whole experimentation, laying hen is carried out conventional immune programme for children, the stress reaction that laying hen causes without disease, environment.
4, test specimen collection and mensuration
When off-test, 2 lethal analysing of chicken bloodletting are got in each repetition at random,, the bursa of farbricius, spleen and cecal tonsil to every chicken, after naked eyes morphological observation, weigh the bursa of farbricius and spleen weight, record.And chicken body weight, the bursa of farbricius and the spleen numerical value of obtained each group are added to full average treatment.To the relative number of the T lymphocyte in thymus gland, the bursa of farbricius, spleen, cecal tonsil, bone-marrow-derived lymphocyte press bibliography (Liu Yubin, Gou Shijin. animal immunology experimental technique. Changchun: Jilin science tech publishing house, 364) method inspection numeration.
In the time that above-mentioned test chicken is raised 35 ages in days, 60 age in days, every group gathers 4 each 0.1ml of anti-freezing collection chicken blood, adds 1.8ml cell culture fluid, adds 1000ug/ml PHA0.1ml simultaneously, and control group does not add PHA, and cell bottle is put to 37 DEG C of 5%CO
2, cultivating 3d, 1 time/d of shake, cultivates while end and inhales and abandon most of supernatant, adds 8.5g/L NH
4cl4m1 mixes, and puts 37% water-bath 10min, the centrifugal 10min of 2500r/min, supernatant discarded, precipitation is fixed liquid 5ml, and the centrifugal 10min of 2500r/min, abandons supernatant, stays the film-making of 0.2ml sedimentation cell, speed dries up, and Ji's nurse Sa dyeing microscopic examination, calculates 200 lymphocytes, calculates percentage.
5, data processing
Data represent with average plus-minus standard deviation, adopt network analysis software SPSS11.5, carry out (LSD) of variance analysis and multiple ratio.
6, results and analysis
6.1 Chinese herbal feed additives detect Chicks ' Growth situation, body weight and immune organ weight
4 cohorts of 60 age in days chicken that add Chinese herbal feed additive are observed, and processed group jumpbogroup chicken grows normal, and feather is neat, and glossiness is good.The indexs such as control group chicken group physique, feather glossiness, the state of mind are all not as test group.Through the relative weights such as chicken body weight and immune organ spleen, the bursa of farbricius are compared, the results detailed in Table 5.
Table 5, respectively organize body weight and immune organ weight
As can be seen from Table 5,3 Chinese herb feed additive interpolation levels are not obvious to chicken weightening finish group difference, but all than control group (407.3g) height.
The impact of 6.2 Chinese herbal feed additives on T lymphocyte, bone-marrow-derived lymphocyte variation in immune organ
T lymphocyte, bone-marrow-derived lymphocyte relative number and control group in the immune organs such as thymus gland to 3 test group and control group chicken, the bursa of farbricius, spleen, the region between the heart and the diaphragm intestinal tonsil body compare, and acquired results is in table 6.
Table 6, respectively organize T in Immune Organs of Chicken, bone-marrow-derived lymphocyte relative number
As shown in Table 6, significant difference (P<0.05), but between test group, difference is not remarkable, illustrates that activation and the Rapid development of Chinese herbal feed additive to Immune Organs of Chicken has obvious facilitation.
To 35 age chicken lymphocyte transformation rate measure, 9% Chinese medicine daily ration group, 10% Chinese medicine daily ration group are respectively 88.42%, 81.0%, have improved 12.6% and 3.18% than control group (78.50%).Analysis of variance, significant difference (P<0.05), what conversion ratio was higher is 9% Chinese medicine daily ration group, the results are shown in Table 7.
The lymphocyte transformation rate of 60 age in days chickens is measured, 7% Chinese medicine daily ration group, 9% Chinese medicine daily ration group are respectively 83%, 84%, improve 9.21% and 10.52% than control group (78.50%), significant difference (P<0.05), what conversion ratio was higher is 9% Chinese medicine daily ration group, the results are shown in Table 7.
Table 7, different tests group chicken T lymphocyte transformation rate (%)
As shown in Table 7, this test has reflected that this Chinese medicine additive can promote lymphopoiesis, conversion, promotes and regulates cellular immunity and humoral immunity, brings into play immunological effect.
Claims (17)
1. a Chinese herbal feed additive, its active component is made up of the Chinese medicine material of following mass parts: blueberry 10-60 mass parts, Poria cocos 10-60 mass parts, marine alga 10-60 mass parts, honeysuckle 10-60 mass parts and oyster 10-60 mass parts.
2. additive according to claim 1, is characterized in that: described Chinese herbal feed additive is made up of the Chinese medicine material of following mass parts: blueberry 40 mass parts, Poria cocos 40 mass parts, marine alga 40 mass parts, honeysuckle 40 mass parts and oyster 40 mass parts.
3. a method of preparing Chinese herbal feed additive described in claim 1 or 2, comprises the steps:
Arbitrary claim 1 or 2 described each Chinese medicine material is mixed according to described mass parts, obtain described Chinese herbal feed additive;
Or, after being mixed according to described mass parts, pulverizes again arbitrary claim 1 or 2 described each Chinese medicine material, obtain described Chinese herbal feed additive.
4. contain the livestock and poultry diet of Chinese herbal feed additive described in claim 1 or 2.
5. livestock and poultry diet according to claim 4, is characterized in that: described fowl is chicken;
Described livestock and poultry diet is daily grain of chicken;
In described livestock and poultry diet, the quality percentage composition of described Chinese herbal feed additive is 7%-10%.
6. livestock and poultry diet according to claim 5, is characterized in that: the quality percentage composition of described Chinese herbal feed additive is 7%-9%.
7. livestock and poultry diet according to claim 5, is characterized in that: the quality percentage composition of described Chinese herbal feed additive is 9%-10%.
8. livestock and poultry diet according to claim 5, is characterized in that: the quality percentage composition of described Chinese herbal feed additive is 9%.
Described in claim 1 or 2 Chinese herbal feed additive in the application of preparing in additive for farm animal feed.
10. application according to claim 9, is characterized in that: described fowl is chicken; Described animal and fowl fodder is daily grain of chicken;
In described animal and fowl fodder, the quality percentage composition of described Chinese herbal feed additive is 7%-10%.
11. application according to claim 10, is characterized in that: the quality percentage composition of described Chinese herbal feed additive is 7%-9%.
12. application according to claim 10, is characterized in that: the quality percentage composition of described Chinese herbal feed additive is 9%-10%.
13. application according to claim 10, is characterized in that: described fowl is chicken; The quality percentage composition of described Chinese herbal feed additive is 9%.
Produce the method for functional Egg, it is characterized in that for 14. 1 kinds: described method comprises the steps:
To livestock and poultry diet described in the claim 5 of feeding in the laying hen of laying period.
15. eggs of being produced by method described in claim 14, described egg is not as propagating materials.
The application of livestock and poultry diet in production functional Egg described in Chinese herbal feed additive or claim 5 described in 16. claims 1 or 2;
Described functional Egg has at least one characteristic in a1-a5;
Wherein, described a1 is that total cholesterol level in described functional Egg is lower than the total cholesterol level in common egg;
Described a2 is that Se content in described functional Egg is higher than the Se content in described common egg;
Described a3 is that iron content in described functional Egg is higher than the iron content in described common egg;
Described a4 is that zinc content in described functional Egg is higher than the zinc content in described common egg;
Described a5 is that the content of DHA in described functional Egg is higher than the DHA content in described common egg;
Described common egg is the egg of producing according to method P:
Described method P is except replacing with the livestock and poultry diet in the method for production functional Egg described in claim 14 the common daily ration of laying cycle of laying hens, and other is all identical with the method for the production functional Egg described in claim 14;
Wherein, the common daily ration of described laying cycle of laying hens is that Chinese herbal feed additive described in the claim 1 or 2 in livestock and poultry diet described in claim 5 is removed to the daily ration obtaining.
Described in 17. claims 1 or 2, described in Chinese herbal feed additive or claim 5, livestock and poultry diet improves the application in following at least one biochemical indicator product in chicken serum in preparation:
1) weight of raising chicken spleen;
2) weight of raising chicken bursa;
3) relative number of T lymphocyte and/or bone-marrow-derived lymphocyte in raising Immune Organs of Chichen;
4) relative number of T lymphocyte and/or bone-marrow-derived lymphocyte in raising chicken spleen;
5) relative number of T lymphocyte and/or bone-marrow-derived lymphocyte in raising chicken bursa;
6) relative number of T lymphocyte and/or bone-marrow-derived lymphocyte in raising cecal tonsil;
7) improve the lymphocytic conversion ratio of T in chicken serum.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310302628.2A CN103330096B (en) | 2013-07-17 | 2013-07-17 | Traditional Chinese medicine feed supplement and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310302628.2A CN103330096B (en) | 2013-07-17 | 2013-07-17 | Traditional Chinese medicine feed supplement and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103330096A CN103330096A (en) | 2013-10-02 |
| CN103330096B true CN103330096B (en) | 2014-11-26 |
Family
ID=49238356
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310302628.2A Active CN103330096B (en) | 2013-07-17 | 2013-07-17 | Traditional Chinese medicine feed supplement and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103330096B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103689260B (en) * | 2013-12-06 | 2016-01-27 | 颍上县润海畜禽养殖有限公司 | A kind of chicken feed improving the immunity of chicken body |
| CN104839434A (en) * | 2015-02-15 | 2015-08-19 | 雷红 | Feed additive and preparation method thereof |
| CN109105330A (en) * | 2017-06-26 | 2019-01-01 | 北京中农雏鹰科技有限公司 | A kind of method of content of taurine in raising egg |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1063603A (en) * | 1992-03-07 | 1992-08-19 | 天津农机化技术推广服务中心 | Reduce the feed addictive of cholesterol level in the egg and chicken |
| CN1172654A (en) * | 1996-08-06 | 1998-02-11 | 徐建生 | Chinese medicinal herb and amino acid feed additive for birds |
| CN101283738A (en) * | 2008-05-29 | 2008-10-15 | 张旭 | Livestock and poultry feed additive made of Chinese-medicinal herbs |
| CN102742725A (en) * | 2012-07-03 | 2012-10-24 | 宁波市鄞州浩斯瑞普生物科技有限公司 | Forage for alleviating chromium poisoning of animals |
| CN103053801A (en) * | 2013-01-22 | 2013-04-24 | 北京众开颜科技有限公司 | Traditional Chinese medicine feed additive and application thereof |
-
2013
- 2013-07-17 CN CN201310302628.2A patent/CN103330096B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1063603A (en) * | 1992-03-07 | 1992-08-19 | 天津农机化技术推广服务中心 | Reduce the feed addictive of cholesterol level in the egg and chicken |
| CN1172654A (en) * | 1996-08-06 | 1998-02-11 | 徐建生 | Chinese medicinal herb and amino acid feed additive for birds |
| CN101283738A (en) * | 2008-05-29 | 2008-10-15 | 张旭 | Livestock and poultry feed additive made of Chinese-medicinal herbs |
| CN102742725A (en) * | 2012-07-03 | 2012-10-24 | 宁波市鄞州浩斯瑞普生物科技有限公司 | Forage for alleviating chromium poisoning of animals |
| CN103053801A (en) * | 2013-01-22 | 2013-04-24 | 北京众开颜科技有限公司 | Traditional Chinese medicine feed additive and application thereof |
Non-Patent Citations (4)
| Title |
|---|
| 伍树松等.花青素作为饲料添加剂的应用前景.《动物营养学报》.2011,第23卷(第12期), * |
| 天然植物饲料添加剂的研究概况;郭旭东等;《粮食与饲料工业》;20071231(第4期);32-34 * |
| 花青素作为饲料添加剂的应用前景;伍树松等;《动物营养学报》;20111231;第23卷(第12期);2097-2104 * |
| 郭旭东等.天然植物饲料添加剂的研究概况.《粮食与饲料工业》.2007,(第4期), * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103330096A (en) | 2013-10-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102100318B (en) | Chicken feed | |
| CN103053801B (en) | Traditional Chinese medicine feed additive and application thereof | |
| CN101715897B (en) | Organic feed for nourishing and body-strengthening layer chickens | |
| CN104054911A (en) | Egg-laying poultry feed additive and egg-laying poultry feed | |
| CN103519009B (en) | Healthcare sand for pigeons | |
| CN105028934A (en) | High-nutrient laying hen feed | |
| CN108201025A (en) | A kind of multifilament cray feed and preparation method thereof | |
| CN103271228A (en) | Bighead feed and production method thereof | |
| CN102987163A (en) | Immune-enhancing feed and preparation method thereof | |
| CN103330096B (en) | Traditional Chinese medicine feed supplement and application thereof | |
| CN104799106A (en) | Insect protein containing protein powder for pets | |
| CN101874566A (en) | Formula of high protein feed | |
| CN103416584B (en) | Method for improving broiler chicken quality by microecologics | |
| CN107455331A (en) | A kind of production method rich in DHA nutrition and health care eggs | |
| CN104431497A (en) | Young parrot powder feed and preparation method thereof | |
| CN104472912A (en) | Young parrot feed and preparation method thereof | |
| CN109793123A (en) | Laying hen multivitamin nutrient selenium-rich zoology feed | |
| KR20210017474A (en) | Method for preparing gelly type of nutritional supplements composition for pet using morinda citrifolia | |
| CN109170134A (en) | A kind of flavor chicken selenium-rich premixed feed and flavor selenium enriched chicken meat production method | |
| CN109497335A (en) | A kind of feed formula and method for breeding that can produce the egg containing tea polyphenols | |
| CN109588552A (en) | Atlantic Ocean seaweed, sargassum, sea lettuce compound feed additive preparation method and application | |
| CN109221621A (en) | A kind of egg feedstuff and preparation method thereof producing high calcium high zinc nutritional egg | |
| CN102715383B (en) | Feed regulation and control agent for producing DHA (docosahexaenoic acid)-rich chicken and feed containing regulation and control agent | |
| CN106343164B (en) | Production method of selenium-rich low-cholesterol eggs | |
| JP2005013091A (en) | Feed for poultry for meat and additive for the same |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address | ||
| CP03 | Change of name, title or address |
Address after: 102206 Beijing city Changping District Baishan Town East West Village profile Patentee after: Beijing haosanyuan Technology Co., Ltd. Address before: 100107, room 705, building 1, Cheong Cheong garden, media village, Beiyuan Road, Beijing, Chaoyang District Patentee before: Beijing Zhongkaiyan Technology Co., Ltd. |