CN106343164B - Production method of selenium-rich low-cholesterol eggs - Google Patents

Production method of selenium-rich low-cholesterol eggs Download PDF

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CN106343164B
CN106343164B CN201610753515.8A CN201610753515A CN106343164B CN 106343164 B CN106343164 B CN 106343164B CN 201610753515 A CN201610753515 A CN 201610753515A CN 106343164 B CN106343164 B CN 106343164B
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cholesterol
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CN106343164A (en
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车勇
辛永强
潘惟谦
张银萍
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Qingdao Pengyang Biological Engineering Co Ltd
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Abstract

The invention discloses a production method of selenium-rich low-cholesterol eggs, which is characterized by comprising the following steps: (1) uniformly mixing 0.5-2 parts by mass of seleno-carrageenan, 8-12 parts by mass of fish meal, 10-14 parts by mass of kelp powder, 9-13 parts by mass of laver powder and 10-14 parts by mass of spirulina powder to prepare the organic selenium high-nutrition feed additive; (2) in 1-2 weeks before the production of the selenium-rich low-cholesterol eggs, the organic selenium high-nutrition feed additive is added into the common ration of the laying poultry in an amount of 0.5wt%, and simultaneously, the selenium-rich nutrient water containing 0.06wt% -0.1 wt% of seleno-carrageenan is fed to the laying poultry, so that the selenium-rich low-cholesterol eggs with the selenium content of more than or equal to 300 mug/kg can be obtained. The invention mixes the raw materials of seleno-carrageenan, fish meal, kelp powder, laver powder, spirulina powder and the like according to a proportion and feeds the mixture to laying poultry to produce green healthy eggs with high selenium content, low cholesterol and no harmful substance residue, which is particularly suitable for people with special requirements such as patients with cardiovascular diseases, hyperlipoproteinemia patients, cholesterolemia patients and the like.

Description

Production method of selenium-rich low-cholesterol eggs
Technical Field
The invention relates to a production method of selenium-rich low-cholesterol eggs.
Background
Selenium is a trace element necessary for human and animals, is an important component of antioxidant enzyme (glutathione peroxidase) and selenium-P protein, plays an important role in human life activities, and can achieve the purposes of improving the immunity of the organism, resisting diseases and delaying senility by supplementing selenium for people. Selenium in human body is mainly taken by food, while the selenium content in the existing edible food is lower. Researches show that the selenium content in meat, eggs and milk of livestock and poultry can be obviously improved by feeding the livestock and poultry with the selenium-rich feed, and the disease resistance of the livestock and poultry is enhanced. At present, inorganic selenium preparation sodium selenite is mainly used by feed production enterprises, has high toxicity and low absorptivity, can cause poisoning when being used carelessly, can generate toxin accumulation in livestock and poultry bodies, and can influence human health after being eaten for a long time; the other kind uses more selenium-enriched yeast, although it is organic selenium, its selenium content is low, its preparation process is tedious and its production cost is high. The selenocarrageenan is a new-generation organic selenium compound formed by combining active polysaccharide extracted from natural seaweed and trace element selenium at a molecular level, has the characteristics of high selenium content, high bioactivity, high bioavailability, no toxic or side effect and the like, is adopted by a plurality of health care products and pharmaceutical factories at home and abroad, and has good application prospect. Therefore, the seleno-carrageenan has important significance as an organic selenium source applied to the aspect of livestock and poultry breeding.
Cholesterol is the most abundant steroid compound in the body, and is a precursor of steroid hormones, bile acids and vitamin D as well as a constituent of cell biofilms. Therefore, it is important for most tissues to ensure the supply of cholesterol and maintain its metabolic balance. Although cholesterol plays an important role in human body, if it is taken in an excessive amount, it causes a series of side effects such as atherosclerosis, hypertension and gallstones, etc., due to metabolic imbalance.
Along with the improvement of living standard of people, more and more attention is paid to living quality and body health, and the food which is rich in nutrition and beneficial to health not only can meet the pursuit of people on health, but also can create huge economic and social benefits. Eggs such as eggs and duck eggs are common food in daily life, but high cholesterol of yolk is often prohibited for patients with cardiovascular diseases, hyperlipoproteinemia and cholesterolemia. The invention aims to research the combination of reducing cholesterol in eggs and improving selenium content, so that the intake of cholesterol is reduced for people, selenium element in human bodies is supplemented, and the selenium-enriched egg is beneficial to human health.
Disclosure of Invention
The invention aims to provide a production method of selenium-rich low-cholesterol eggs, which is characterized in that raw materials such as seleno-carrageenan, fish meal, kelp powder, laver powder and spirulina powder are mixed according to a proportion, and the raw materials are fed to laying poultry by utilizing the synergistic effect of different components and proportions to produce green healthy eggs with high selenium content, low cholesterol and no harmful substance residue, so as to meet the requirements of people on high-quality eggs, and the eggs are particularly suitable for people with special requirements such as patients with cardiovascular diseases, patients with hyperlipoproteinemia and people with cholesterolemia to eat.
The technical scheme of the invention is as follows:
A production method of selenium-rich low-cholesterol eggs is characterized by comprising the following steps:
(1) Uniformly mixing 0.5-2 parts by mass of seleno-carrageenan, 8-12 parts by mass of fish meal, 10-14 parts by mass of kelp powder, 9-13 parts by mass of laver powder and 10-14 parts by mass of spirulina powder to prepare the organic selenium high-nutrition feed additive;
(2) in 1-2 weeks before the production of the selenium-rich low-cholesterol eggs, the organic selenium high-nutrition feed additive is added into the common ration of the laying poultry in an amount of 0.5wt%, and simultaneously, the selenium-rich nutrient water containing 0.06wt% -0.1 wt% of seleno-carrageenan is fed to the laying poultry, so that the selenium-rich low-cholesterol eggs with the selenium content of more than or equal to 300 mug/kg can be obtained.
The selenium content of the seleno-carrageenan is 1 wt%.
The seleno-carrageenan in the formula has the selenium-rich effect; the spirulina powder has the function of enhancing immunity; fish powder, herba Zosterae Marinae powder and thallus Porphyrae powder have cholesterol reducing effect.
The selenocarrageenan is added with the four substances of fish meal, kelp powder, laver powder and spirulina powder in proportion, so that the selenium enrichment effect is more obvious; and the fish meal, the kelp powder, the laver powder and the seleno-carrageenan are added in proportion, so that the effect of reducing the cholesterol in the egg yolk is more remarkable.
The feed additive disclosed by the invention has the following functions and functions:
1. The seleno carrageenan (also called as seaweed seleno polysaccharide) is a novel organic selenium compound which takes natural sulfate polysaccharide extracted from marine algae as a matrix and adopts an accurate molecular docking technology to ensure that trace element selenium and active polysaccharide are tightly jointed in a molecular state, and has a plurality of physiological functions of integrating the active polysaccharide and the trace element selenium necessary for human bodies. As a selenium nutrition enhancer, the selenium-enriched. The active polysaccharide has physiological effects in many aspects, has outstanding expression in the aspects of immunoregulation, radiation resistance, intestine and stomach regulation, tissue structure regeneration or repair assistance, wound healing promotion and the like, and can promote the absorption of nutrients by laying fowls and improve the growth performance. Selenium has important physiological functions in the processes of antioxidant protection and apoptosis of cells, and particularly in the processes of embryonic development and spermatogenesis, selenium is very important for maintaining a proper antioxidant system. The effectiveness of selenium in responding to stress conditions during poultry production cannot be underestimated. From the viewpoint of pre-hatch egg storage, selenium is involved in counteracting the destructive effects of free radicals and toxic metabolic components. The organic selenium in the daily feed is one of the main measures for maintaining the high yield and reproductive performance of the poultry. The selenium carrageenan is added into the daily ration, so that the concentration of selenium in the eggs can be obviously improved, and the daily ration organic selenium level has high correlation with the content of selenium in the eggs.
2. Fish meal is a high-protein feed raw material which is prepared by using one or more fishes as raw materials and carrying out deoiling, dehydration and crushing processing. The fish meal does not contain substances such as cellulose and the like which are difficult to digest, has high crude fat content and high effective energy value, and is easy to prepare high-energy feed by taking the fish meal as a raw material in production. The fish meal is rich in B vitamins, particularly high in vitamin B12 and B2, and also contains fat-soluble vitamins such as vitamin A, D, vitamin E and the like. Fish meal is a good source of minerals, has high contents of calcium and phosphorus and proper proportion, and all phosphorus can be utilized. In addition, the fish meal has high content of iodine, zinc and iron and contains a proper amount of arsenic. Fish meal contains unknown factors for promoting growth, and the substances can stimulate the growth and development of animals although the substances are not purified into compounds.
3. the kelp is a vegetable with high nutritive value and has certain medicinal value. It is rich in iodine and other mineral elements. The kelp has low calorie, moderate protein content and rich mineral substances, and researches show that the kelp has multiple biological functions of reducing blood fat, reducing blood sugar, regulating immunity, resisting coagulation, resisting tumors, expelling lead, detoxifying, resisting oxidation and the like. The kelp contains a large amount of unsaturated fatty acid and dietary fiber, can remove cholesterol attached to the vessel wall, regulate and smooth intestines and stomach, promote the excretion of cholesterol, and the addition of kelp powder in the daily ration of egg-laying poultry can reduce the accumulation of cholesterol in eggs, thereby producing low-cholesterol eggs.
4. The porphyra belongs to red algae plants, grows on shallow sea rock, has 3 colors of red purple, green purple and black purple, is purple after being dried, and is named as the porphyra because the porphyra can be used as the vegetables. The laver is nutritious, has high iodine content, and contains polysaccharide capable of remarkably reducing total content of serum cholesterol. Therefore, the addition of the laver powder in the daily ration is beneficial to the production of low-cholesterol eggs.
5. Spirulina is a type of prokaryote, a filament consisting of single or multiple cells, cylindrical, bent in a loose or tight regular spiral, shaped like a clockwork spring, hence its name. The spirulina has the functions of regulating blood sugar, enhancing immune system, protecting intestines and stomach, and the like.
The invention has the following advantages:
1. The seleno-carrageenan is an organic selenium source, has high selenium content, high biological activity, high bioavailability and no toxic or side effect, but can cause listlessness and hair fading of poultry when being singly used as a selenium-rich egg selenium source supplement for feeding the poultry for a long time. Meanwhile, the selenium content in the eggs can reach more than 300 mug/kg, and the accumulation of poultry toxin caused by adding inorganic selenium is avoided.
2. the fish meal, kelp powder and laver powder are common marine products, and are applied to feeding the eggs and poultry, and the cholesterol content in the eggs and poultry is reduced compared with that in the eggs which are not added. In addition, compared with the test poultry which only adds fish meal, kelp powder and laver powder, the seleno-carrageenan, the fish meal, the kelp powder and the laver powder are simultaneously fed to the egg poultry, and the cholesterol content in the egg products produced by the poultry is obviously reduced.
3. The seleno-carrageenan is prepared from a marine product carrageenan, and belongs to homologous substances with fish meal, kelp powder, laver powder and spirulina powder. Compared with the experimental poultry which are only added with the seleno-carrageenan in the feed, the eggs produced by the poultry which are simultaneously added with the fish meal, the kelp powder, the laver powder and the spirulina powder have obviously improved selenium content; therefore, the synergistic effect of the selenocarrageenan and the marine product powder is important for producing the selenium-rich low-cholesterol eggs.
Detailed Description
The present invention will be further described with reference to the following specific examples.
Example 1: influence of different feeding methods on selenium content and cholesterol content in eggs
Test site: qingdao flatness smooth-going farm
360 160-day-old Hailan laying hens in the same batch are selected in the test, and the hens are strong in physique, similar in weight and similar in egg laying performance. Laying hens are equally divided into six groups, each group is fed with basic ration, and the conditions of other additives are carried out according to the following table 1:
TABLE 1 feeding methods of each group
In the test process, the laying hens in the second group and the fifth group have hair loss after being fed for 20 days, and are accompanied with listlessness, and no hair loss occurs in other groups. The selenium content and the cholesterol content in the yolk of each group of eggs were detected on the 10 th day, the 20 th day and the 30 th day of feeding respectively, and the detection results are shown in table 2:
TABLE 2 Effect of different feeding methods on selenium content in eggs
TABLE 3 Effect of different feeding methods on the cholesterol content of egg yolk
From the test phenomena in combination with tables 2 and 3, the following conclusions can be drawn:
1. as can be seen from the test data, the laying hens in the groups 2 and 5 have hair loss after being fed for 20 days, which is accompanied with listlessness, while the group 6 of the combination group 3 has no such phenomenon, which proves that the immunity of the chicken can be enhanced by adding the spirulina powder, the adverse effect of the seleno-carrageenan on the laying hens can be eliminated, and the absorption of the selenium element in the eggs is not influenced.
2. the selenium content in the eggs is obviously improved compared with the group without adding the selenocarrageenan, which indicates that the selenocarrageenan has good selenium-rich effect as the selenium source of the selenium-rich eggs; and as can be seen from the 6 th group of selenium-enriched data, the combination of the fish meal, the kelp powder, the laver powder and the spirulina powder has a gain effect on the selenium-enriched effect of the selenocarrageenan.
3. The 4 th group, the 5 th group and the 6 th group are added with the fish meal, the kelp powder and the laver powder, compared with other three groups, the cholesterol content in the egg yolk is obviously reduced, which shows that the cholesterol content in the egg can be reduced by adding the fish meal, the kelp powder and the laver powder; and compared with the 5 th group and the 4 th group which are added with the seleno-carrageenan, the cholesterol is reduced more obviously, which shows that the seleno-carrageenan, fish meal, kelp powder and laver powder have promotion effect on reducing the cholesterol content in the egg yolk.
Example 2: production method of selenium-rich low-cholesterol eggs
1. Test site: qingdao flatness smooth-going farm
2. The test method comprises the following steps:
(1) Uniformly mixing 1 part of seleno-carrageenan, 9 parts of fish meal, 13 parts of kelp powder, 13 parts of laver powder and 14 parts of spirulina powder to prepare the organic selenium high-nutrition feed additive, wherein the selenium content of the seleno-carrageenan is 1 wt%; preparing organic selenium nutrient water containing 0.08 wt% of seleno-carrageenan by taking seleno-carrageenan.
(2) 180 160-day-old Hailan laying hens in the same batch are selected in the test, and the hens are strong in physique, similar in weight and similar in egg laying performance. The test group is divided into 2 groups of 90 chickens at random, and the groups are respectively a control group and a test group, each group is provided with 6 effective repeats, and each effective repeat is provided with 15 chickens for data comparison in the test group. The test is carried out for 7 days, and each group is fed with basic feed. The test period is 30 days, the first 10 days are the initial period of the test, the middle 10 days are the middle period of the test, and the last 10 days are the final period of the test. In the test period, the test group adds 0.5wt% of organic selenium high-nutrition feed additive into basic ration, and feeds organic selenium nutrient water containing 0.08 wt% of seleno-carrageenan, while the control group only feeds basic ration, and other feeding time and amount are carried out according to the original feeding management program.
(3) And recording the feed intake, the egg yield, the total egg weight, the broken egg number and the like of each group of test chickens every day by taking the repetition as a unit, and calculating the egg yield, the average egg weight, the feed-egg ratio and the breakage rate according to the record. 2 eggs were collected in each group repeatedly at the initial stage, the middle stage and the last day of the experiment, and the selenium content and the yolk cholesterol content were measured.
3. And (3) test results:
Test results show that the production performance index of the experimental group laying hens is improved by adding 0.5wt% of the organic selenium high-nutrition feed additive into the basic daily ration and feeding 0.08 wt% of organic selenium nutrient water containing seleno-carrageenan (see table 4). The selenium content in the eggs of the test group rapidly increased with increasing test time, reaching 300. mu.g/kg after 10 days, and the cholesterol content in the egg yolk was significantly reduced (see tables 5 and 6).
TABLE 4 influence of different feeding methods on the production Performance of egg-laying hens
TABLE 5 Effect of different feeding methods on selenium content in eggs
TABLE 6 Effect of different feeding methods on the cholesterol content of egg yolk
example 3: production method of selenium-rich low-cholesterol duck eggs
1. Test site: qingdao Hamming farm
2. the test method comprises the following steps:
(1) Uniformly mixing 1.2 parts of seleno-carrageenan, 10.5 parts of fish meal, 12 parts of kelp powder, 12.8 parts of laver powder and 13.5 parts of spirulina powder to prepare the organic selenium high-nutrition feed additive, wherein the seleno-carrageenan contains 1wt% of selenium; preparing organic selenium nutrient water containing 0.07 wt% of seleno-carrageenan by using seleno-carrageenan.
(2) 180 ducks which lay eggs in the same batch at the age of 150 days are selected in the test, the body constitution is strong, the body weight is similar, and the egg laying performance is similar. The duck breeding group is divided into 90 ducks in 2 groups at random, wherein the groups are respectively a control group and a test group, each group is provided with 6 effective repeats, and each effective repeat is provided with 15 ducks for data comparison in the test group. The test is carried out for 7 days, and each group is fed with basic feed. The test period is 30 days, the first 10 days are the initial period of the test, the middle 10 days are the middle period of the test, and the last 10 days are the final period of the test. In the test period, the test group adds 0.5wt% of organic selenium high-nutrition feed additive into basic ration, and feeds organic selenium nutrient water containing 0.07 wt% of seleno-carrageenan, while the control group only feeds basic ration, and other feeding time and amount are carried out according to the original feeding management program.
(3) And recording the feed intake, the egg yield, the total egg weight, the broken egg number and the like of each group of test ducks every day by taking the repetition as a unit, and calculating the egg yield, the average egg weight, the feed-egg ratio and the breakage rate according to the record. 2 duck eggs are repeatedly collected in each group in the initial stage, the middle stage and the last day of the final stage of the experiment, and the selenium content and the yolk cholesterol content are detected.
3. And (3) test results:
Test results show that the production performance index of experimental egg-laying ducks is improved by adding 0.5wt% of organic selenium high-nutrition feed additive into basic daily ration and feeding 0.07 wt% of organic selenium nutrient water containing seleno-carrageenan (see table 7). The selenium content in the duck eggs in the test group rapidly increased with increasing test time, and reached 400. mu.g/kg after 10 days, and the cholesterol content in the egg yolk was significantly reduced (see tables 8 and 9).
TABLE 7 influence of different feeding methods on the egg-laying duck production performance
TABLE 8 Effect of different feeding methods on selenium content in duck eggs
TABLE 9 Effect of different feeding methods on the cholesterol content of egg yolk
Finally, it is also noted that the above-mentioned lists merely illustrate some specific embodiments of the invention. Those skilled in the art will appreciate that modifications, additions and substitutions are possible, without departing from the scope of the invention as disclosed in the accompanying claims.

Claims (4)

1. A production method of selenium-rich low-cholesterol eggs is characterized by comprising the following steps:
(1) uniformly mixing 0.5-2 parts by mass of seleno-carrageenan, 8-12 parts by mass of fish meal, 10-14 parts by mass of kelp powder, 9-13 parts by mass of laver powder and 10-14 parts by mass of spirulina powder to prepare the organic selenium high-nutrition feed additive;
(2) In 1-2 weeks before the production of the selenium-rich low-cholesterol eggs, the organic selenium high-nutrition feed additive is added into the common ration of the laying poultry in an amount of 0.5wt%, and simultaneously, the selenium-rich nutrient water containing 0.06wt% -0.1 wt% of seleno-carrageenan is fed to the laying poultry, so that the selenium-rich low-cholesterol eggs with the selenium content of 300-461 mug/kg can be obtained.
2. The method for producing selenium-enriched low-cholesterol eggs according to claim 1, characterized in that the selenium content of the seleno-carrageenan is 1 wt%.
3. The method for producing selenium-rich low-cholesterol eggs according to claim 1, characterized in that the organic selenium high-nutrition feed additive comprises the following components by mass: 1 part of selenocarrageenan, 9 parts of fish meal, 13 parts of kelp powder, 13 parts of laver powder and 14 parts of spirulina powder.
4. The method for producing selenium-rich low-cholesterol eggs according to claim 1, characterized in that the organic selenium high-nutrition feed additive comprises the following components by mass: 1.2 parts of selenocarrageenan, 10.5 parts of fish meal, 12 parts of kelp powder, 12.8 parts of laver powder and 13.5 parts of spirulina powder.
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