CN103330096A - Traditional Chinese medicine feed supplement and application thereof - Google Patents
Traditional Chinese medicine feed supplement and application thereof Download PDFInfo
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Abstract
The invention discloses a traditional Chinese medicine feed supplement and an application thereof. The traditional Chinese medicine feed supplement, which is livestock and poultry feed supplement, comprises the following traditional Chinese medicine raw materials by mass part: 10-60 parts of blueberry, 10-60 parts of Indian buead, 10-60 parts of marine algae, 10-60 parts of honeysuckle and 310-60 parts of oyster. The livestock and poultry feed supplement can reduce the egg cholesterol level, improve the content of selenium, iron and zinc of the egg, as well as the DHA content of the egg and weight of spleen and bursa of fabricius, enhance the relative number of T and B lymphocyte in tonsillas in the thymus, spleen, bursa of fabricius and caecal, and improve the T lymphocyte conversion rate of blood serum of the egg.
Description
Technical field
The present invention relates to a kind of additive for farm animal feed, particularly a kind of Chinese herbal feed additive and application thereof.
Background technology
Quick and the intensivization development of modern poultry husbandry, chicken mass-sending disease is also frequent day by day, influences egg quality.Use a large amount of antibiotic, cause the medicament residue of egg to increase.Use Chinese herb feed additive, meet the use standard of nuisanceless medicine, can not only ward off disease, and contain a large amount of nutriments, chicken group nutrition and egg product matter are improved greatly.
DHA(Docosahexaenoic acid, i.e. DHA) be the long-chain polyunsaturated fatty acid (ω-3PUFAs) of a kind of ω of belonging to-3 series.It is one of main component of human brain, accounts for about 10% of people's cephalopin.DHA can not be synthesized in human body, has only from external world's absorption and replenishes, and marine fishes, Mycophyta and seaweeds etc. are its main sources.Studies show that in a large number DHA has brain nourishing and eyesight improving, angiocardiopathy preventing, anticancer, anti-inflammatory and suppresses function such as allergy, is the important nutrient of keeping human health.If can improve DHA content in the egg, has important function for keeping and improving human health
Cholesterol can be divided into HDL-C (HDL-cholesterol) and LDL-C (LDL-cholesterol) according to its function.The HDL-cholesterol has cleaning artery impurity, prevents arteriosclerotic effect, to human body favourable " good cholesterol "; The LDL-cholesterol then is the important evocator that causes disease.But the very important high nutritive peculiarity of traditional eating habit and egg is doomed its status in people's diet and is difficult to shake, and how to eliminate the key that the edible taboo of egg becomes the egg healthy edible.For this reason, reduce cholesterol level in the egg, to improve that cholesterol forms be the effectively section of having that addresses the above problem.
Functional Egg refer to except nutritious delicious, regulate the egg of human physiological activity's function in addition.In recent years, common functional Egg has both at home and abroad:
1 iodine-enriched egg
Iodine plays an important role in keeping thyroid gland normal function, carbohydrate and lipid metabolism as a kind of trace element of needed by human.Big quantity research report, nutritious iodine-enriched egg is the health food that prevents the iodine deficiency disease, and treatment hyperthyroidism, thyroid gland enlargement, coronary heart disease etc. are had excellent curative.Kind (1993) for a long time report on the mountain, the Thallus Laminariae (Thallus Eckloniae) extract of interpolation 2% is when (containing iodine 0.38%) in the laying hen material, and content of iodine increases by 2.1～5.8mg in per 100 gram yolk; When adding 4%, the content of iodine is about 2 times that add at 2% o'clock in the yolk; When adding 6%, the content of iodine is about 2.5 times of interpolation 2%.Improve addition again, the content of iodine just can not be significantly increased in the yolk, keeps above-mentioned level substantially.
2 high zinc eggs
Recent study report, the egg of high-load zinc to prevention and treatment owing to lack the disease that zinc causes, as child and teenager's maldevelopment, wound healing slowly, anaemia etc. has good clinical effectiveness.Dai Lan etc. (1994) test shows, uses certain density zinc solution to mix and stir in feed, and the 15d that feeds can make test group egg zinc content improve 67.7% than the egg zinc content of control group.Xu Jianxiong etc. (1994) report, in feed, add 1000,2000, behind the 3000mg/kg zinc in the 15d yolk zinc content improve 34.22%, 46.9% and 75.71% respectively.
The high lecithin egg of 3 low cholesterols
The research report, cellulose can adsorb cholate in enteron aisle, shorten cholesterol by the time of enteron aisle, thereby reduces the absorption of cholesterol.Rybina (1985) is increased to 15% with clover in the feed, and cholesterol level only is original 66.7%～50% in the yolk as a result.Wang Yucai etc. (1992) mix 2% fiber when feeding laying hen as additive with chelating agent, reduced cholesterol level very significantly.
4 green eggs
Along with China's entry into the World Trade Organization, the exploitation of pollution-free food and production more and more are subjected to people's attention.Green egg is one of pollution-free food type, pollution-free, the noresidue of producing of referring to raise chickens, to the harmless egg of health.
Summary of the invention
The purpose of this invention is to provide a kind of Chinese herbal feed additive and application thereof.
Chinese herbal feed additive provided by the invention, its active component is made by the Chinese medicine material of following mass parts: blueberry 10-60 mass parts, Poria cocos 10-60 mass parts, marine alga 10-60 mass parts, honeysuckle 10-60 mass parts and oyster 10-60 mass parts.
The active component of described Chinese herbal feed additive specifically can be made up of the Chinese medicine material of following mass parts: blueberry 40 mass parts, Poria cocos 40 mass parts, marine alga 40 mass parts, honeysuckle 40 mass parts and oyster 40 mass parts.
Described mass parts is dry weight.
In the above-mentioned Chinese medicine material, described blueberry is the ripening fruits of Ericaceae Vaccinium plant blueberry; Described Poria cocos is Polyporaceae fungi Poria cocos Poria cocos(Schw.) dry sclerotia of Wolf; Described marine alga is the dwell herb of dish or Sargassum of Sargassaceae plant sheep; Described honeysuckle is the dry flower of caprifoliaceae plant honeysuckle Lonicera japonica Thunb.; Described oyster is the shell for the long oyster Ostrea of Ostreidae animal gigas Thunberg, ostrea talienwhanensis Crosse Ostrea talienwhanensis Crosse or Crassostrea rivularis Ostrea rivularis Gould.
Described Chinese medicine material can be Chinese medicine, also can be the prepared slices of Chinese crude drugs.
Described Chinese herbal feed additive can only be made up of active component, also can add other animal and fowl fodder component again.
Above-mentioned Chinese herbal feed additive is the product for getting according to following method preparation provided by the invention also.
The method of the described Chinese herbal feed additive of preparation provided by the invention comprises the steps:
Described each Chinese medicine material according to described mass parts mixing, is obtained described Chinese herbal feed additive;
Perhaps, described each Chinese medicine material is pulverized after according to described mass parts mixing again, obtained described Chinese herbal feed additive.
Contain the livestock and poultry diet of above-mentioned any Chinese herbal feed additive and described Chinese herbal feed additive as the application in the additive for farm animal feed, also belong to protection scope of the present invention.
Described fowl is specially chicken;
Described livestock and poultry diet is daily grain of chicken;
Described daily grain of chicken can be used as the laying cycle of laying hens daily ration, also can be used as the daily ration in other period.
The mass content of additive for farm animal feed described in the described daily grain of chicken can be 9%, 9%-10%, 7%-10% or 7%-9%.
The application of above-mentioned daily grain of chicken in the production functional Egg also belongs to protection scope of the present invention.
Described functional Egg has at least a characteristic among the a1-a5;
Wherein, described a1 is that total cholesterol level in the described functional Egg is lower than the total cholesterol level in the common egg;
Described a2 is that the Se content in the described functional Egg is higher than the Se content in the described common egg;
Described a3 is that the iron content in the described functional Egg is higher than the iron content in the described common egg;
Described a4 is that the zinc content in the described functional Egg is higher than the zinc content in the described common egg;
Described a5 is that the content of DHA in the described functional Egg is higher than the DHA content in the described common egg;
Described common egg is the egg of producing according to method P:
The contain described daily grain of chicken of described method P in the method that will produce described functional Egg replaces with the common daily ration of laying cycle of laying hens, and other is all identical with the method for producing described functional Egg;
Wherein, the common daily ration of described laying cycle of laying hens is that the arbitrary described Chinese herbal feed additive in the described daily grain of chicken is removed the daily ration that obtains.
Wherein, for the production of the quality percentage composition of additive for farm animal feed described in the described daily grain of chicken with described a1 characteristic can be 9%, 9%-10%, 7%-10% or 7%-9%;
For the production of the quality percentage composition of additive for farm animal feed described in the described daily grain of chicken with described a2 characteristic can be 9%, 9%-10%, 7%-10% or 7%-9%;
For the production of the quality percentage composition of additive for farm animal feed described in the described daily grain of chicken with described a3 characteristic can be 9%, 9%-10%, 7%-10% or 7%-9%;
For the production of the quality percentage composition of additive for farm animal feed described in the described daily grain of chicken with described a4 characteristic can be 9%, 9%-10%, 7%-10% or 7%-9%;
For the production of the quality percentage composition of additive for farm animal feed described in the described daily grain of chicken with described a4 characteristic can be 9%, 9%-10%, 7%-10% or 7%-9%;
For the production of the quality percentage composition of additive for farm animal feed described in the described daily grain of chicken with described a5 characteristic can be 9%, 9%-10%, 7%-10% or 7%-9%.
The common daily ration of described laying cycle of laying hens is not except containing described additive for farm animal feed, and other raw material all can be identical with routine laying period daily ration, as contain energy feed, protein feeds, mineral feed and vitamin feed; Further, described common laying period,, feed also can contain antioxidant.The common daily ration of described laying cycle of laying hens can be made up of energy feed, protein feeds, mineral feed and vitamin feed, also can be formed by energy feed, protein feeds, mineral feed, vitamin feed and antioxidant, certainly, can also in described common laying period feed, add non-nutritive additives such as anticorrisive agent, colouring agent, flavor enhancement, medicinal health-care agent, growth promoter again.
Wherein, described energy feed can be chicken energy feed commonly used, and as being selected from least a in the following substances: paddy reality, bran, potato, grass-seed and tree are real etc.
Described protein feeds can be egg white matter feed commonly used, as being selected from least a in the following substances: big dregs of beans, the cottonseed dregs of rice, rapeseed dregs, fish meal, rapeseed dregs, peanut meal and the certain herbaceous plants with big flowers dregs of rice etc.
Described mineral feed can be selected from stone flour, calcium bicarbonate, salt, oyster shell whiting, bone meal, calcium monohydrogen phosphate, calcium carbonate, salt, copper sulphate, ferrous sulfate, manganese sulfate, KI, zinc oxide, sodium selenite and cobalt chloride etc.
The present invention also provides a kind of method of production functional Egg.
The method of production functional Egg provided by the present invention comprises to the laying hen that the is in laying period above-mentioned daily grain of chicken of feeding obtaining the step of described functional Egg.
It is described that to give the laying hen the be in laying period above-mentioned daily grain of chicken of feeding be to the laying hen that is in laying period (21-72 age in week, as 21-24 age in week, 30-37 age in week, 25-42 age in week, 43-50 age in week) the above-mentioned daily grain of chicken more than 14 days (as 14-21 days, 42 days, 14 days-42 days, 14-35 days) of feeding.
Egg by said method production also belongs to protection scope of the present invention, and described egg is as propagating materials, as only as food and/or health products.
The present invention also protects described additive for farm animal feed and described livestock and poultry diet to improve the application in the following at least a biochemical indicator product in the chicken serum in preparation:
1) weight of raising chicken spleen;
2) weight of raising chicken bursa;
3) relative number of T lymphocyte and/or bone-marrow-derived lymphocyte in the raising pigeon breast gland;
4) relative number of T lymphocyte and/or bone-marrow-derived lymphocyte in the raising chicken spleen;
5) relative number of T lymphocyte and/or bone-marrow-derived lymphocyte in the raising chicken bursa;
6) relative number of T lymphocyte and/or bone-marrow-derived lymphocyte in the raising cecal tonsil;
7) improve the lymphocytic conversion ratio of T in the chicken serum.
Wherein, 1), 2) 3) 4) 5) 6) and 7) the quality percentage composition of additive for farm animal feed described in the used daily grain of chicken can be 9%, 10%, 7%, 9%-10%, 7%-10% or 7%-9%;
Described product can be the chicken feed.
Additive for farm animal feed of the present invention can reduce egg inner cholesterol content; The 2nd, can improve selenium, iron and the zinc content of egg; The 3rd, can improve the content of DHA in the egg; The 4th, can improve the weight of spleen and the bursa of farbricius; The 5th, can strengthen the relative number of T, bone-marrow-derived lymphocyte in thymus gland, spleen, the bursa of farbricius and the cecal tonsil; The 6th, improve T lymphocyte transformation rate in the chicken serum.Additive for farm animal feed of the present invention is because adopting the Chinese medicine of integration of drinking and medicinal herbs, so to livestock and poultry animal and human without any side effect.The development green livestock and poultry already satisfies people's food security, improves nutritive value of food, strengthens livestock products in the competitiveness of international market, has important economic implications and social benefit.
The specific embodiment
Following embodiment is convenient to understand better the present invention, but does not limit the present invention.Experimental technique among the following embodiment if no special instructions, is conventional method.Used material, reagent etc. if no special instructions, all can obtain from commercial channels among the following embodiment.
In the Chinese medicine material of following embodiment, blueberry is the ripening fruits of Ericaceae Vaccinium plant blueberry, Poria cocos is Polyporaceae fungi Poria cocos Poria cocos(Schw.) dry sclerotia of Wolf, marine alga is the dwell herb of dish or Sargassum of Sargassaceae plant sheep, honeysuckle is the dry flower of caprifoliaceae plant honeysuckle Lonicera japonica Thunb., and oyster is the shell for the long oyster Ostrea of Ostreidae animal gigas Thunberg, ostrea talienwhanensis Crosse Ostrea talienwhanensis Crosse or Crassostrea rivularis Ostrea rivularis Gould.
The production method of the functional Egg of embodiment 1, DHA content height, low, the rich selenium of total cholesterol level, iron and zinc
One, preparation Chinese herbal feed additive
After blueberry, Poria cocos, marine alga, honeysuckle and the oyster of integration of drinking and medicinal herbs pulverized respectively, proportioning according to blueberry 40 mass parts, Poria cocos 40 mass parts, marine alga 40 mass parts, honeysuckle 40 mass parts and oyster 40 mass parts is mixed, and obtains Chinese herbal feed additive.
Two, production functional Egg
1, laying period daily ration
Laying period,, daily ration was divided into following 4 kinds:
1.1 laying period common daily ration
Its composition is as shown in table 1.
The common daily ration of table 1, laying period is formed and trophic level
| Component | Unit (gram) | ? | Trophic level |
| Corn | 63.00 | Metabolizable energy/Jkg-1 | 10852.73 |
| Dregs of beans | 24.00 | Thick protein/% | 17.6 |
| Stone flour | 8.00 | Coarse ash % | 12.94 |
| Premix | 5.00 | Crude fibre/% | 5.8 |
| ? | ? | Calcium/% | 4 |
| ? | ? | Total phosphorus/% | 1 |
| ? | ? | Available phosphorus/% | 0.62 |
| ? | ? | NaCl | 0.5 |
| ? | ? | Lysine | 1.50 |
| ? | ? | Methionine | 0.79 |
| ? | ? | Methionine+cystine | 0.80 |
| ? | ? | Threonine | 1.00 |
Wherein, contain vitamin A 550000U in every kilogram of premix, vitamin D
3162000U, vitamin E
1100U, vitamin K1 51mg, pantothenic acid 410mg, folic acid 45mg, biotin 4.5mg, Choline Chloride 20g, Cobastab
1120mg, Cobastab
2295mg, Cobastab
666mg, Cobastab
120.25mg; Trace element contains Fe4g, Zn4.1g, Mn4.9g, Cu0.4g, I75mg, Se18mg.
Wherein, vitamin A is in vitamin(e) A acetate, then 1IU vitamin A=0.344 μ g vitamin(e) A acetate;
Vitamin D
3In Vitamin D3,1IU vitamin D then
3=0.025 μ g Vitamin D3;
Vitamin E
1In alpha-tocopherol, 1IU vitamin E then
1=0.668mg alpha-tocopherol.
1.27% Chinese medicine daily ration.
In 1.1 laying period common daily ration, add step 1 gained Chinese herbal feed additive, addition be this laying period common daily ration quality 7%, obtain 7% Chinese medicine daily ration.
1.39% Chinese medicine daily ration.
In 1.1 laying period common daily ration, add step 1 gained Chinese herbal feed additive, addition be this laying period common daily ration quality 9%, obtain 9% Chinese medicine daily ration.
1.410% Chinese medicine daily ration.
In 1.1 laying period common daily ration, add step 1 gained Chinese herbal feed additive, addition be this laying period common daily ration quality 10%, obtain 10% Chinese medicine daily ration.
2, experimental animal
Age in days, physique amount, laying period healthy sea blue grey laying hen 96 plumages close with laying rate are selected in test for use, be divided into 4 processed group at random: common daily ration group of laying period (abbreviation control group), 7% Chinese medicine daily ration group, 9% Chinese medicine daily ration group, 10% Chinese medicine daily ration group, every group of 6 repetitions, each repeats 4 chickens.
3, test method
With above-mentioned 4 processed group respectively with corresponding laying period daily ration raised in advance 7 days, formally fed 28 days, test chicken 8:00 every day and 18:00 feeding, free choice feeding, the nipple-shape water dispenser is freely drunk water, illumination every day 16h, keeps air quality in the house at gravity-flow ventilation.In each gets 6 pieces in egg sample repeating at random from raising beginning the 14th day, 24 days, 28 days in advance, after the sampling egg carried out mark and put into 4 ℃ of refrigerators and preserve in experimental period.
Wherein, common daily ration group of laying period (abbreviation control group) with above-mentioned laying period common daily ration feed, 7% Chinese medicine daily ration group is fed with above-mentioned 7% Chinese medicine daily ration, and 9% Chinese medicine daily ration group is fed with above-mentioned 9% Chinese medicine daily ration, and 10% Chinese medicine daily ration group is fed with above-mentioned 10% Chinese medicine daily ration.Other raising condition is all identical.
Wherein, in the whole experiment, laying hen is carried out conventional immune programme for children, and laying hen does not have the stress reaction of disease, environment initiation.
4, testing index and method
4.1 performance test
Duration of test, every day, laying rate, egg quality and the feed intake of chicken respectively organized in record, calculates average egg quality, laying rate and feedstuff-egg ratio, and formula is as follows:
Average egg weight=every group of total egg quality/every group of number of laying eggs,
Laying rate=the number of laying eggs/livestock on hand chicken number * 100%,
Feedstuff-egg ratio=feed consumption rate/total nitrogen quality
4.2 the mensuration of egg product matter
Measure egg size, egg shape index, shell thickness, eggshell weight, Ha Shi unit, yolk color: the Zhou Gui of every piece of egg sample, Wang Like, Huang Ruihua etc. according to the method for following document.Production of Livestock and Poultry experiment study course. China Agricultyre University Press .2006.
4.3 egg Determination of nutritional components
Yolk and the albumen of egg sample are stirred evenly, smash, in 65 ℃ of baking ovens, dry 5～6h, after the taking-up, in room air, cool off 4h, weigh, make air-dry sample.Measure thick protein, phosphatide, vitamin A, selenium, iron, zinc, vitamin E: GB5009.5-2010, GB/T5009.128-2003, GB/T5009.82-2003 according to the method in the following standard.
The egg sample is boiled, get yolk and measure cholesterol level: Zhou Gui according to the method for following document, Wang Like,, Huang Ruihua etc.Production of Livestock and Poultry experiment study course. China Agricultyre University Press, 2006.
DHA: with reference to (Li Jings such as Li Jings, Deng Zeyuan, topaz China. the mensuration of aliphatic acid [J] in the eggs. University Of Nanchang's journal: natural sciences version, 2007,31 (2): 164-16) method is measured DHA content in the egg, and the mensuration of this experimental data is tested laboratory, Co., Ltd Beijing by the spectrum Buddhist nun and finished.
EPA: measure EPA content in the egg with reference to GB/T541327-2007, the mensuration of this experimental data is tested laboratory, Co., Ltd Beijing by the spectrum Buddhist nun and is finished.
5, data are handled
Test data adopts SAS software to carry out variance analysis, and Duncan ' s method is carried out multiple ratio, and the result represents with " mean+SD ".
6, result and analysis
6.1 Chinese herbal feed additive is to the influence of egg product matter
Chinese herbal feed additive sees Table 2 to the influence of egg product matter.
The egg quality of table 2, each group
| Project | Control group | 7% Chinese medicine daily ration group | 9% Chinese medicine daily ration group | 10% Chinese medicine daily ration group |
| Average egg weight/g | 64.72±0.33 b | 64.97±1.83 b | 69.46±0.47 a | 67.89±1.37 b |
| Laying rate/% | 84.32±1.32 b | 86.50±1.50 a | 88.36±1.05 b | 87.72±1.24 a |
| Feedstuff-egg ratio | 2.33±0.93 b | 2.31±0.86 ab | 2.22±0.58 a | 2.28±0.74 ab |
| Ha Shi unit | 78.45±4.71 a | 79.57±4.70 a | 79.59±3.72 a | 79.69±6.43 a |
| Shell thickness/mm | 0.41±0.03 a | 0.43±0.076 a | 0.43±0.04 a | 0.42±0.03 a |
| Eggshell weight/g | 7.51±0.14 a | 7.42±0.27 a | 7.37±0.13 a | 7.38±0.31 a |
| Yolk color | 7.44±0.48 b | 7.57±0.75 b | 8.57±0.37 a | 8.41±0.89 ab |
| The egg shape index | 0.75±0.06 a | 0.76±0.02 a | 0.77±0.04 a | 0.76±0.08 a |
As shown in Table 2, along with the increase of Chinese herbal feed additive addition, egg size and laying rate increase gradually, and the 9% Chinese medicine daily ration group average egg weight utmost point is significantly higher than other groups (P＜0.01), feedstuff-egg ratio is lower than other processed group, and other respectively handle group difference not significantly (P〉0.05);Along with the increase yolk color of addition is deepened, 9% Chinese medicine daily ration group color is the darkest, compares significant difference (P＜0.05) with control group, does not have significant difference (P〉0.05) between other each processed group and the control group.Different interpolation levels is heavy to egg shape index, Ha Shi unit, shell thickness, eggshell, do not have significantly influence, difference not significantly (P〉0.05) between each group.
6.2 Chinese herbal feed additive is to the influence of part nutrient composition content in the egg
Chinese herbal feed additive sees Table 3 to the influence of part nutrient composition content in the egg.
Table 3, Chinese herbal feed additive are to the influence of part nutrient composition content in the egg
By table 3 as seen, the content of each test group amino acid and phosphatide and control group all do not have significant difference (P〉0.05).Compare with control group, the total cholesterol level in 7% Chinese medicine daily ration group, 9% Chinese medicine daily ration group and the 10% Chinese medicine daily ration group egg significantly decreases, and wherein 9% Chinese medicine daily ration group total cholesterol level descends 69.70% with respect to control group.Compare with control group, selenium, iron, zinc content in 7% Chinese medicine daily ration group, 9% Chinese medicine daily ration group and the 10% Chinese medicine daily ration group egg obviously raise.
DHA(Docosahexaenoic acid, i.e. DHA) be the long-chain polyunsaturated fatty acid (ω-3PUFAs) of a kind of ω of belonging to-3 series.It is one of main component of human brain, accounts for about 10% of people's cephalopin.DHA can not be synthesized in human body, has only from external world's absorption and replenishes, and marine fishes, Mycophyta and seaweeds etc. are its main sources.Studies show that in a large number DHA has brain nourishing and eyesight improving, angiocardiopathy preventing, anticancer, anti-inflammatory and suppresses function such as allergy, is the important nutrient of keeping human health.
As shown in Table 3, compare with control group, the DHA content in 7% Chinese medicine daily ration group, 9% Chinese medicine daily ration group and the 10% Chinese medicine daily ration group egg obviously raises (P＜0.05), but the EPA content of egg the inside is not influenced.
Embodiment 2, Chinese herbal feed additive are to the influence of laying hen serum biochemistry index and immunity
1, daily ration
Daily ration is divided into following 4 kinds:
1.1 common daily ration
Its composition is as shown in table 4.
Table 4, common daily ration are formed and trophic level
| Component | Unit (gram) | ? | Trophic level |
| Corn | 59.1 | Metabolizable energy/MJkg-1 | 11.71 |
| Dregs of beans | 25 | Thick protein/% | 15.9 |
| Wheat bran | 5 | Calcium/% | 3.49 |
| Stone flour | 9 | Available phosphorus/% | 0.60 |
| Calcium monohydrogen phosphate | 1.4 | Methionine/% | 0.31 |
| Salt | 0.39 | ? | ? |
| Methionine | 0.13 | ? | ? |
| B B-complex * | 0.02 | ? | ? |
| Trace element * * | 0.2 | ? | ? |
Wherein, every kilogram of B B-complex contains vitamin A 550000U, vitamin D
3162000U, vitamin E
1100U, vitamin K1 51mg, pantothenic acid 410mg, folic acid 45mg, biotin 4.5mg, Choline Chloride 20g, Cobastab
1120mg, Cobastab
2295mg, Cobastab
666mg, Cobastab
120.25mg all the other are stone flour.Every kilogram of trace element contains Fe4g, Zn4.1g, and Mn4.9g, Cu0.4g, I75mg, Se18mg, all the other are stone flour.
1.27% Chinese medicine daily ration.
Add in 1.1 common daily ration is that the Chinese herbal feed additive of 7% embodiment 1 of this common daily ration quality obtains 7% Chinese medicine daily ration.In the 7% Chinese medicine daily ration, the quality percentage composition of Chinese herbal feed additive is 7%.
1.39% Chinese medicine daily ration.
Add in 1.1 common daily ration is that the Chinese herbal feed additive of 10% embodiment 1 of this common daily ration quality obtains 9% Chinese medicine daily ration.In the 9% Chinese medicine daily ration, the quality percentage composition of Chinese herbal feed additive is 9%.
1.410% Chinese medicine daily ration.
Add in 1.1 common daily ration is that the Chinese herbal feed additive of 10% embodiment 1 of this common daily ration quality obtains 10% Chinese medicine daily ration.In the 10% Chinese medicine daily ration, the quality percentage composition of Chinese herbal feed additive is 10%.
2, experimental animal
Test selects for use same hen house with 320 of 14 age in days Luo Man laying hens of a collection of health, adopt the single-factor RANDOMIZED BLOCK DESIGN, laying hen is divided into 4 processed group at random: common daily ration group (abbreviation control group), 7% Chinese medicine daily ration group, 9% Chinese medicine daily ration group, 10% Chinese medicine daily ration group, each handles 5 repetitions, and each repeats 16 chickens.
3, test method
Above-mentioned 4 processed group were raised 7 days in advance with corresponding daily ration respectively, formally fed 60 days, test chicken 8:00 every day and 18:00 feeding, free choice feeding, the nipple-shape water dispenser is freely drunk water, illumination every day 16h, air quality in gravity-flow ventilation, the maintenance house.
Wherein, common daily ration group (abbreviation control group) is fed with above-mentioned common daily ration, 7% Chinese medicine daily ration group is fed with above-mentioned 7% Chinese medicine daily ration, and 9% Chinese medicine daily ration group is fed with above-mentioned 9% Chinese medicine daily ration, and 10% Chinese medicine daily ration group is fed with above-mentioned 10% Chinese medicine daily ration.Other raising condition is all identical.
Wherein, in the whole experiment, laying hen is carried out conventional immune programme for children, and laying hen does not have the stress reaction of disease, environment initiation.
4, test specimen collection and mensuration
During off-test, each repeats to get at random 2 chicken bloodletting and causes death and to analyse,, to the bursa of farbricius, spleen and the cecal tonsil of every chicken behind the naked eyes morphological observation, the weighing bursa of farbricius and spleen weight, record.And to obtained each the group chicken body weight, the bursa of farbricius and spleen numerical value add full average treatment.To the relative number of the T lymphocyte in thymus gland, the bursa of farbricius, spleen, the cecal tonsil, bone-marrow-derived lymphocyte press list of references (Liu Yubin, Gou Shijin. the animal immunology experimental technique. Changchun: Jilin science tech publishing house, 364) method inspection numeration.
When above-mentioned test chicken is raised 35 ages in days, 60 ages in days, gather 4 each 0.1ml of anti-freezing collection chicken blood for every group, add the 1.8ml cell culture fluid, add 1000ug/ml PHA0.1ml simultaneously, control group does not add PHA, and the cell bottle is put 37 ℃ of 5%CO
2, cultivate 3d, shake 1 time/d, cultivate to inhale when finishing and abandon most of supernatant, add 8.5g/L NH
4The Cl4m1 mixing is put 37% water-bath 10min, the centrifugal 10min of 2500r/min, supernatant discarded, precipitation adds fixer 5ml, and the centrifugal 10min of 2500r/min abandons supernatant, stays the film-making of 0.2ml sedimentation cell, speed dries up, and Ji's nurse Sa dyeing microscopic examination calculates 200 lymphocytes, calculates percentage.
5, data are handled
Data are represented with average plus-minus standard deviation, adopt network analysis software SPSS11.5, carry out variance analysis and multiple ratio (LSD).
6, result and analysis
6.1 Chinese herbal feed additive detects chick growth developmental state, body weight and immune organ weight
4 cohorts of 60 age in days chickens that add Chinese herbal feed additive are observed, and processed group jumpbogroup chicken grows normal, and feather is neat, and glossiness is good.Indexs such as control group chicken group physique, feather glossiness, the state of mind are all not as test group.Through relative weights such as chicken body weight and immune organ spleen, the bursa of farbricius are compared, the result sees table 5 for details.
Table 5, respectively to organize body weight and immune organ heavy
As can be seen from Table 5,3 Chinese herb feed additive interpolation levels are not obvious to chicken weightening finish group difference, but all than control group (407.3g) height.
6.2 the influence that Chinese herbal feed additive changes T lymphocyte, bone-marrow-derived lymphocyte in the immune organ
T lymphocyte, bone-marrow-derived lymphocyte relative number and control group in the immune organs such as the thymus gland of 3 test group and control group chicken, the bursa of farbricius, spleen, the region between the heart and the diaphragm intestinal tonsil body are compared, and gained the results are shown in Table 6.
Table 6, respectively organize T in the chicken immune organ, bone-marrow-derived lymphocyte relative number
As shown in Table 6, significant difference (P＜0.05), but difference is not remarkable between the test group, Chinese herbal feed additive is described to the activation of chicken immune organ and grows fast to have obvious facilitation.
To 35 age chicken lymphocyte transformation rate measure, 9% Chinese medicine daily ration group, 10% Chinese medicine daily ration group are respectively 88.42%, 81.0%, have improved 12.6% and 3.18% than control group (78.50%).Through variance analysis, significant difference (P＜0.05), what conversion ratio was higher is 9% Chinese medicine daily ration group, the results are shown in Table 7.
Lymphocyte transformation rate to 60 age in days chickens is measured, and 7% Chinese medicine daily ration group, 9% Chinese medicine daily ration group are respectively 83%, 84%, improved 9.21% and 10.52% than control group (78.50%), significant difference (P＜0.05), what conversion ratio was higher is 9% Chinese medicine daily ration group, the results are shown in Table 7.
Table 7, different tests group chicken T lymphocyte transformation rate (%)
As shown in Table 7, this test has reflected that this Chinese medicine additive can promote lymphopoiesis, conversion, promotes and adjusting cellular immunity and humoral immunity the performance immunological effect.
Claims (10)
1. Chinese herbal feed additive, its active component is made up of the Chinese medicine material of following mass parts: blueberry 10-60 mass parts, Poria cocos 10-60 mass parts, marine alga 10-60 mass parts, honeysuckle 10-60 mass parts and oyster 10-60 mass parts.
2. additive according to claim 1, it is characterized in that: described Chinese herbal feed additive is made up of the Chinese medicine material of following mass parts: blueberry 40 mass parts, Poria cocos 40 mass parts, marine alga 40 mass parts, honeysuckle 40 mass parts and oyster 40 mass parts.
3. additive according to claim 1 and 2 is characterized in that: the product of described Chinese herbal feed additive for getting according to the described method preparation of claim 4.
4. a method for preparing claim 1 or 2 described Chinese herbal feed additives comprises the steps:
Claim 1 or 2 arbitrary described each Chinese medicine material according to described mass parts mixing, are obtained described Chinese herbal feed additive;
Perhaps, claim 1 or 2 arbitrary described each Chinese medicine material are pulverized after according to described mass parts mixing again, obtained described Chinese herbal feed additive.
5. the livestock and poultry diet that contains arbitrary described Chinese herbal feed additive among the claim 1-3; Or,
The arbitrary described Chinese herbal feed additive of claim 1-3 is as the application in the additive for farm animal feed.
6. livestock and poultry diet according to claim 5 or application is characterized in that: described fowl is chicken;
Described livestock and poultry diet is daily grain of chicken;
In the described livestock and poultry diet, the quality percentage composition of described Chinese herbal feed additive is 9%, 9%-10%, 7%-10% or 7%-9%.
7. arbitrary described Chinese herbal feed additive or claim 5 or 6 application of described livestock and poultry diet in the production functional Egg among the claim 1-3;
Described functional Egg has at least a characteristic among the a1-a5;
Wherein, described a1 is that total cholesterol level in the described functional Egg is lower than the total cholesterol level in the common egg;
Described a2 is that the Se content in the described functional Egg is higher than the Se content in the described common egg;
Described a3 is that the iron content in the described functional Egg is higher than the iron content in the described common egg;
Described a4 is that the zinc content in the described functional Egg is higher than the zinc content in the described common egg;
Described a5 is that the content of DHA in the described functional Egg is higher than the DHA content in the described common egg;
Described common egg is the egg of producing according to method P:
The contain claim 6 described daily grain of chicken of described method P in the method that will produce described functional Egg replaces with the common daily ration of laying cycle of laying hens, and other is all identical with the method for producing described functional Egg;
Wherein, the common daily ration of described laying cycle of laying hens is that arbitrary described Chinese herbal feed additive among the claim 1-4 in the described daily grain of chicken of claim 6 is removed the daily ration that obtains.
8. method of producing functional Egg, it is characterized in that: described method comprises the steps:
Give the laying hen the be in laying period described daily grain of chicken of claim 6 of feeding.
9. by the egg of the described method production of claim 8, described egg is not as propagating materials.
10. arbitrary described Chinese herbal feed additive, the described daily grain of chicken of claim 6 improve the application in the following at least a biochemical indicator product in the chicken serum in preparation among the claim 1-3:
1) weight of raising chicken spleen;
2) weight of raising chicken bursa;
3) relative number of T lymphocyte and/or bone-marrow-derived lymphocyte in the raising pigeon breast gland;
4) relative number of T lymphocyte and/or bone-marrow-derived lymphocyte in the raising chicken spleen;
5) relative number of T lymphocyte and/or bone-marrow-derived lymphocyte in the raising chicken bursa;
6) relative number of T lymphocyte and/or bone-marrow-derived lymphocyte in the raising cecal tonsil;
7) improve the lymphocytic conversion ratio of T in the chicken serum.
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