CN103316187A - Traditional chinese medicine extract - Google Patents
Traditional chinese medicine extract Download PDFInfo
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- CN103316187A CN103316187A CN2013102525029A CN201310252502A CN103316187A CN 103316187 A CN103316187 A CN 103316187A CN 2013102525029 A CN2013102525029 A CN 2013102525029A CN 201310252502 A CN201310252502 A CN 201310252502A CN 103316187 A CN103316187 A CN 103316187A
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Abstract
The invention relates to a traditional Chinese medicine extract which is prepared from Scutellaria baicalensis and Chinese yam decocting concentrated extract, baicalin, Chinese yam ethanol extract and Chinese yam polysaccharide according to the ratio of (1-3):(6-9):(3-7):(4-6). The composition has a good curative effect on associated diarrhea of cancer chemotherapy, and meanwhile can play synergism on cancer chemotherapy.
Description
Technical field
The present invention relates to a kind of Chinese medicine extract, relate more specifically to a kind of Chinese medicine extract that is comprised of Radix Scutellariae Rhizoma Dioscoreae water boiling concentration extract, baicalin, Rhizoma Dioscoreae ethanol extraction and Chinese yam polysaccharide, the preparation method of this Chinese medicine extract and this Chinese medicine extract are for the preparation of reducing the cancer chemotherapy induced diarrhea and treatment of cancer being played purposes in the medicine of potentiation.
Background technology
Malignant tumor is current harm humans existence, one of healthy principal disease, and its treatment means comprises operation, chemotherapy, radiotherapy, Chinese medicine, and the effect of chemotherapeutic treatment tumor is particularly outstanding.Chemotherapeutic is no matter alone or unite with the toxicity such as all can cause that the patient suffers from diarrhoea, and diarrhoea is again Chemotherapy-induced Diarrhea behind the chemotherapy of tumors.In recent years emerging and applying along with chemotherapeutics such as 5-fluorouracil, taxanes, hydroxy camptothecin, irinotecan, xeloda etc., the incidence rate of Chemotherapy-induced Diarrhea improves year by year, if do not carry out active treatment, easily cause patient's dehydration, malnutrition, serum electrolyte disorder, acid base imbalance etc., it is unfavorable greatly not only the rehabilitation of body to be brought, also bring certain difficulty to CC, therefore effective treatment of Chemotherapy-induced Diarrhea has been become the important component part of tumor patient wholistic therapy.
Chemotherapy-induced Diarrhea is with regard to its clinical manifestation, surely belongs to the Chinese medicine category of " having loose bowels ", and Chinese medicine attracts people's attention for the research of having loose bowels, but there is no at present the Chinese patent medicine that the antagonism cancer chemotherapy has alleviating diarrhoea and potentiation.Therefore, research and develop a kind of high-efficiency low-toxicity, cheap, the natural Chinese medicine that can be used for alleviating the cancer chemotherapy induced diarrhea and playing the chemotherapeutic sensitivity effect that is easy to promote becomes clinical problem demanding prompt solution.
Radix Scutellariae is the root of labiate Radix Scutellariae, cures mainly dysentery, and Rhizoma Dioscoreae is perennial voluble herb, and Chinese medicine, vegetable dual-purpose have therapeutical effect to diarrhea due to hypofunction of the spleen.In recent years, Radix Scutellariae and Rhizoma Dioscoreae antitumaous effect are confirmed, the inventor is through research and the experiment of each a large amount of formula proportion, obtained at last optimal drug ratio compositions of the present invention, and proved that it has fabulous curative effect and can simultaneously to chemotherapeutic sensitivity, finish thus the present invention the cancer chemotherapy induced diarrhea.
Summary of the invention
A kind of Chinese medicine extract provided by the invention is made of in the ratio of 1-3:6-9:3-7:4-6 by weight Radix Scutellariae Rhizoma Dioscoreae water boiling concentration extract, baicalin, Rhizoma Dioscoreae ethanol extraction and Chinese yam polysaccharide.
Above-mentioned Chinese medicine extract, wherein the usage ratio of Radix Scutellariae Rhizoma Dioscoreae water boiling concentration extract, baicalin, Rhizoma Dioscoreae ethanol extraction and Chinese yam polysaccharide is 3:9:7:6.
Wherein, Radix Scutellariae Rhizoma Dioscoreae water boiling concentration extract, baicalin, Rhizoma Dioscoreae ethanol extraction can be by well known to a person skilled in the art the conventional method acquisition of purifying, and the natural drug of the present invention that these compositions that conventional method obtains or the commercial one-tenth of buying are grouped into all can obtain identical curative effect.
Preferably, the preparation method of Radix Scutellariae Rhizoma Dioscoreae water boiling concentration extract is in the above-mentioned Chinese medicine extract:
Be 1-4 according to proportion compatibility: 1 gets Radix Scutellariae, Rhizoma Dioscoreae, distilled water immersion, and heating decocts 1h, and silk filters, and medicinal residues are adding distil water again, soaks, decocts, filters with method, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, 70 ℃ of drying under reduced pressure and get final product.
The preparation method of baicalin is in the above-mentioned Chinese medicine extract:
The Radix Scutellariae oven dry, crushing screening, 80% ethanol heating extraction twice, merging filtrate, Recycled ethanol, add an amount of distilled water, add concentrated hydrochloric acid and transfer pH3-4, leave standstill 1h after-filtration decontamination, filtrate is transferred pH1.5-2 in 40 ℃, insulation 30min, sucking filtration, precipitation is washed to pH6.5-7.0, is drying to obtain.
Rhizoma Dioscoreae ethanol extraction preparation method is in the above-mentioned Chinese medicine extract:
Get the Rhizoma Dioscoreae powder, 95% alcohol reflux 3 times removes supernatant, filtering residue extracts 4 times at 45 ℃ with distilled water, collects extracting solution, and is centrifugal, collect supernatant, with supernatant concentration, add ethanol, 4 ℃ of placements are spent the night, centrifugal, collecting precipitation is used respectively ethanol, acetone, ether washing precipitation, lyophilization, and get final product.
The Chinese yam polysaccharide preparation method is in the above-mentioned Chinese medicine extract:
Above-mentioned Rhizoma Dioscoreae ethanol extraction is added the water redissolution be made into 5% solution, add Sevag reagent, concussion, centrifugal, get supernatant, add Sevag reagent, so repeatedly, until without white intermediate layer precipitation, collect supernatant concentration, use respectively tap water and distill water dialysis, add ethanol, 4 ℃ of placements are spent the night, centrifugal, collecting precipitation is used respectively ethanol, acetone and ether washing precipitation, lyophilization, and get final product.
The present invention also provides the preferred preparation method of this Chinese medicine extract, comprising:
(1) preparation of Radix Scutellariae Rhizoma Dioscoreae water boiling concentration extract
Be 1-4 according to proportion compatibility: 1 gets Radix Scutellariae, Rhizoma Dioscoreae, distilled water immersion, and heating decocts 1h, and silk filters, and medicinal residues are adding distil water again, soaks, decocts, filters with method, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, 70 ℃ of drying under reduced pressure and get final product.
(2) preparation of baicalin
The Radix Scutellariae oven dry, crushing screening, 80% ethanol heating extraction twice, merging filtrate, Recycled ethanol, add an amount of distilled water, add concentrated hydrochloric acid and transfer pH3-4, leave standstill 1h after-filtration decontamination, filtrate is transferred pH1.5-2 in 40 ℃, insulation 30min, sucking filtration, precipitation is washed to pH6.5-7.0, is drying to obtain.
(3) Rhizoma Dioscoreae ethanol extraction preparation
Get the Rhizoma Dioscoreae powder, 95% alcohol reflux 3 times removes supernatant, filtering residue extracts 4 times at 45 ℃ with distilled water, collects extracting solution, and is centrifugal, collect supernatant, with supernatant concentration, add ethanol, 4 ℃ of placements are spent the night, centrifugal, collecting precipitation is used respectively ethanol, acetone, ether washing precipitation, lyophilization, and get final product.
(4) Chinese yam polysaccharide preparation
Above-mentioned Rhizoma Dioscoreae ethanol extraction is added the water redissolution be made into 5% solution, add Sevag reagent, concussion, centrifugal, get supernatant, add Sevag reagent, so repeatedly, until without white intermediate layer precipitation, collect supernatant concentration, use respectively tap water and distill water dialysis, add ethanol, 4 ℃ of placements are spent the night, centrifugal, collecting precipitation is used respectively ethanol, acetone and ether washing precipitation, lyophilization, and get final product.
(5) choose in proportion Radix Scutellariae Rhizoma Dioscoreae water boiling concentration extract, baicalin, Rhizoma Dioscoreae ethanol extraction and Chinese yam polysaccharide, evenly mix, and get final product.
The preparation method of above-mentioned Chinese medicine extract also comprises: the product that step (5) makes is further made capsule or the tablet that contains Chinese medicine extract 100mg or 200mg specification by the pharmacy means of routine.
The beneficial effect of Chinese medicine extract of the present invention aspect anti-Chemotherapy-induced Diarrhea and inhibition tumor is as follows:
1, Chinese medicine extract of the present invention causes studies show that of diarrhea of mouse model at irinotecan: the diarrhoea reaction for the irinotecan induced mice has obvious antagonism.
2, proved that this Chinese medicine extract can strengthen chemotherapeutics to the inhibitory action of tumor, adopted mouse tumor model, observed this Chinese medicine extract and cyclophosphamide combined to the inhibitory action of mouse interior tumor.Studies show that: this Chinese medicine extract can obviously increase cyclophosphamide for the inhibitory action of tumor.
3, the inventor is originally with Radix Scutellariae Rhizoma Dioscoreae water boiling concentration extract, baicalin, Rhizoma Dioscoreae ethanol extraction and Chinese yam polysaccharide make up, and determine consumption proportion between these components by great many of experiments, make said composition bring into play good synergism, through experimentation repeatedly, the ratio of each component of Chinese medicine extract of the present invention has produced unexpected synergism when 1-3:6-9:3-7:4-6, potentiation is obvious, extraordinary effect is played in side effect, especially especially obvious when the usage ratio of each component is 3:9:7:6, this is that the inventor pays a large amount of creative works and obtains, and those of ordinary skills only can't simply obtain this ratio range and optimum amount ratio by disclosed content.
4, this Chinese medicine extract Radix Scutellariae Rhizoma Dioscoreae water boiling concentration extract, baicalin, Rhizoma Dioscoreae ethanol extraction and each component of Chinese yam polysaccharide all can obtain by conventional means, component such as baicalin and Chinese yam polysaccharide also can be buied by commerce, and such compositions itself has the convenience of acquisition, lower-cost effect.This also is the product that has splendid curative effect and higher industrial practicality concurrently that the inventor obtains by a large amount of creative work sides.
The specific embodiment
Further set forth the preparation of Chinese medicine extract of the present invention below by embodiment and test example, with and the beneficial effect in diarrhea and antitumor, used.
Embodiment
The first step: the preparation of various extracts
(1) preparation of Radix Scutellariae Rhizoma Dioscoreae water boiling concentration extract
Be 1-4 according to proportion compatibility: 1 gets Radix Scutellariae, Rhizoma Dioscoreae, distilled water immersion, and heating decocts 1h, and silk filters, and medicinal residues are adding distil water again, soaks, decocts, filters with method, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, 70 ℃ of drying under reduced pressure and get final product.
(2) preparation of baicalin
The Radix Scutellariae oven dry, crushing screening, 80% ethanol heating extraction twice, merging filtrate, Recycled ethanol, add an amount of distilled water, add concentrated hydrochloric acid and transfer pH3-4, leave standstill 1h after-filtration decontamination, filtrate is transferred pH1.5-2 in 40 ℃, insulation 30min, sucking filtration, precipitation is washed to pH6.5-7.0, is drying to obtain.
(3) Rhizoma Dioscoreae ethanol extraction preparation
Get the Rhizoma Dioscoreae powder, 95% alcohol reflux 3 times removes supernatant, filtering residue extracts 4 times at 45 ℃ with distilled water, collects extracting solution, and is centrifugal, collect supernatant, with supernatant concentration, add ethanol, 4 ℃ of placements are spent the night, centrifugal, collecting precipitation is used respectively ethanol, acetone, ether washing precipitation, lyophilization, and get final product.
(4) Chinese yam polysaccharide preparation
Above-mentioned Rhizoma Dioscoreae ethanol extraction is added the water redissolution be made into 5% solution, add Sevag reagent, concussion, centrifugal, get supernatant, add Sevag reagent, so repeatedly, until without white intermediate layer precipitation, collect supernatant concentration, use respectively tap water and distill water dialysis, add ethanol, 4 ℃ of placements are spent the night, centrifugal, collecting precipitation is used respectively ethanol, acetone and ether washing precipitation, lyophilization, and get final product.
Second step: the preparation of preparation
(1) preparation of Chinese medicine extract capsule preparations of the present invention
Take by weighing respectively Radix Scutellariae Rhizoma Dioscoreae water boiling concentration extract 12mg, baicalin 36mg, Rhizoma Dioscoreae ethanol extraction 28mg and the Chinese yam polysaccharide 24mg of above-mentioned steps preparation, take by weighing simultaneously starch 395mg, magnesium stearate 5mg, evenly mix, be filled to capsule.
(2) preparation of Chinese medicine extract tablet of the present invention
Take by weighing respectively Radix Scutellariae Rhizoma Dioscoreae water boiling concentration extract 12mg, baicalin 36mg, Rhizoma Dioscoreae ethanol extraction 28mg and the Chinese yam polysaccharide 24mg of above-mentioned steps preparation, take by weighing simultaneously starch 295mg, lactose 100mg, evenly mix, with wet granulation, drying, mix with the 5mg magnesium stearate behind the granulate, be pressed into tablet.
Drug effect and pharmacology's data:
Compositions is in following test example: the usage ratio of Radix Scutellariae Rhizoma Dioscoreae water boiling concentration extract, baicalin, Rhizoma Dioscoreae ethanol extraction and Chinese yam polysaccharide is 3:9:7:6.Inventor's compositions that the multiple proportioning combination between 1-3:6-9:3-7:4-6 the time obtains to above-mentioned each composition usage ratio carried out with on identical experiment, find that all the Chinese medicine extract in this amount ranges can obtain good synergism, can play good effect and the treatment of cancer is had potentiation the cancer chemotherapy induced diarrhea.Below be the pharmacodynamics data that preferred embodiment obtains:
Test example 1: the pharmacodynamic study of Chinese medicine extract treatment Chemotherapy-induced Diarrhea effect
1, test material
(1) Radix Scutellariae, Rhizoma Dioscoreae is purchased from medical material market.
(2) mice, body weight 20 ± 2g, male, ad lib drinking-water before the experiment.
2, test method:
Mice is divided into 5 groups at random, and 5 every group, the A group is model group, and the B group is Radix Scutellariae Rhizoma Dioscoreae water boiling concentration extract 10g.kg
-1(being equivalent to contain the crude drug amount), the C group is baicalin 10g.kg
-1(being equivalent to contain the crude drug amount), D group Rhizoma Dioscoreae ethanol extraction mixes 10g.kg with Chinese yam polysaccharide in the 7:6 ratio
-1(being equivalent to contain the crude drug amount), the E group is Chinese medicine extract 10g.kg of the present invention
-1(being equivalent to contain the crude drug amount), irinotecan intraperitoneal injection (75mg/kg), continuous 4 days, copies the Chemotherapy-induced Diarrhea model at every day 1 time.B, C, D, E group with corresponding extract gavage 8 days, places metabolic cage with mice continuously, spreads white filter paper at the bottom of the cage observing the stool situation, and observes mice crissum situation and Mus tail fecal pollution situation, and in conjunction with cotton swab stimulation anus defecation, every day, observed and recorded was 2 times.Diarrhoea degree standard scoring is as follows: 0 minute: stool is normal or do not have; 1 minute: laxativeness, stool is visible slight wet soft; 2 minutes: moderate diarrhoea, stool wet and not molding.And there is slight crissum painted; 3 minutes, severe diarrhoea, watery stool is also painted with the severe crissum.
3, experimental result
Compare with model group, Chinese medicine extract group Incidence of Diarrhea of the present invention obviously alleviates.The results are shown in Table 1.
Table 1 present composition is on mice Chemotherapy-induced Diarrhea incidence rate and scoring impact
Annotate: a is equivalent to contain the crude drug amount
Test example 2: the pharmacodynamic study of Chinese medicine extract antitumor action of the present invention
1, experiment material
(1) Kunming mouse, body weight 20 ± 2g, ad lib drinking-water before the experiment, 24h natural light irradiation.
(2) cyclophosphamide, the permanent auspicious medical Pharmacy stock Co., Ltd in Jiangsu product.
2, test method:
To mice S
180The inhibition test of sarcoma growth
Employing was gone down to posterity 5-7 days, and well-grown milky ascites is diluted to tumor cell suspension with an amount of physiological saline solution, and cell number is 10
7/ mL, 6 every group, the right axillary fossa subcutaneous vaccination of every Mus 0.2mL(contains oncocyte 2 * 10
6Individual).The inoculation next day random packet, experimental group Chinese medicine extract of the present invention is divided into (200,100mg/mL) two dosage groups, every Mus gavage 0.5 ml gives cyclophosphamide 10gkg simultaneously
-1D
-1Negative control group gives 0.5 mL distilled water; The positive drug group gives cyclophosphamide 10gkg
-1D
-1, successive administration 10 days.The conventional raising, drinking-water, food is not limit.Drug withdrawal next day, mice all takes off cervical vertebra puts to death, and strips subcutaneous solid tumor tumor piece, weighs, and calculates tumor control rate (%) by following formula:
Tumor control rate=(the average tumor of the matched group average tumor of heavy (C)-experimental group heavy (T))/average tumor of matched group heavy (C)
3, result of the test
Chinese medicine extract of the present invention is low, high dose group all can strengthen the cyclophosphamide agent with to S
180The effect of inhibition degree, especially the strongest with the high dose group effect.(such as table 2)
Table 2 Chinese medicine extract of the present invention is to S
180The tumor inhibition effect of mice
| Group | Suppression ratio (%) |
| Cyclophosphamide | 31.5 |
| Compositions is low+cyclophosphamide | 37.1 |
| Height+cyclophosphamide | 48.5 |
Claims (5)
1. one kind is reduced the cancer chemotherapy induced diarrhea and the treatment cancer is played the Chinese medicine extract of potentiation, it is characterized in that said composition is comprised of Radix Scutellariae Rhizoma Dioscoreae water boiling concentration extract, baicalin, Rhizoma Dioscoreae ethanol extraction and the Chinese yam polysaccharide ratio in 1-3:6-9:3-7:4-6
Wherein the preparation method of Radix Scutellariae Rhizoma Dioscoreae water boiling concentration extract is:
Be 1-4 according to proportion compatibility: 1 gets Radix Scutellariae, Rhizoma Dioscoreae, distilled water immersion, and heating decocts 1h, and silk filters, and medicinal residues are adding distil water again, soaks, decocts, filters with method, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, 70 ℃ of drying under reduced pressure and get final product;
Wherein the preparation method of baicalin is:
The Radix Scutellariae oven dry, crushing screening, 80% ethanol heating extraction twice, merging filtrate, Recycled ethanol, add an amount of distilled water, add concentrated hydrochloric acid and transfer pH3-4, leave standstill 1h after-filtration decontamination, filtrate is transferred pH1.5-2 in 40 ℃, insulation 30min, sucking filtration, precipitation is washed to pH6.5-7.0, is drying to obtain;
Wherein the preparation method of Rhizoma Dioscoreae ethanol extraction is:
Get the Rhizoma Dioscoreae powder, 95% alcohol reflux 3 times removes supernatant, filtering residue extracts 4 times at 45 ℃ with distilled water, collects extracting solution, and is centrifugal, collect supernatant, with supernatant concentration, add ethanol, 4 ℃ of placements are spent the night, centrifugal, collecting precipitation is used respectively ethanol, acetone, ether washing precipitation, lyophilization, and get final product;
Wherein the preparation method of Chinese yam polysaccharide is:
Above-mentioned Rhizoma Dioscoreae ethanol extraction is added the water redissolution be made into 5% solution, add Sevag reagent, concussion, centrifugal, get supernatant, add Sevag reagent, so repeatedly, until without white intermediate layer precipitation, collect supernatant concentration, use respectively tap water and distill water dialysis, add ethanol, 4 ℃ of placements are spent the night, centrifugal, collecting precipitation is used respectively ethanol, acetone and ether washing precipitation, lyophilization, and get final product.
2. Chinese medicine extract as claimed in claim 1 is characterized in that wherein the usage ratio of Radix Scutellariae Rhizoma Dioscoreae water boiling concentration extract, baicalin, Rhizoma Dioscoreae ethanol extraction and Chinese yam polysaccharide is 3:9:7:6.
3. such as the preparation method of the Chinese medicine extract of claim 1 or 2, it is characterized in that the method comprises:
(1) preparation of Radix Scutellariae Rhizoma Dioscoreae water boiling concentration extract
Be 1-4 according to proportion compatibility: 1 gets Radix Scutellariae, Rhizoma Dioscoreae, distilled water immersion, and heating decocts 1h, and silk filters, and medicinal residues are adding distil water again, soaks, decocts, filters with method, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, 70 ℃ of drying under reduced pressure and get final product;
(2) preparation of baicalin
The Radix Scutellariae oven dry, the oven dry of powder Radix Scutellariae, crushing screening, 80% ethanol heating extraction twice, merging filtrate, Recycled ethanol adds an amount of distilled water, adds concentrated hydrochloric acid and transfers pH3-4, leaves standstill 1h after-filtration decontamination, and filtrate is transferred pH1.5-2 in 40 ℃, insulation 30min, sucking filtration, precipitation is washed to pH6.5-7.0, is drying to obtain;
(3) preparation of Rhizoma Dioscoreae ethanol extraction
Get the Rhizoma Dioscoreae powder, 95% alcohol reflux 3 times removes supernatant, filtering residue extracts 4 times at 45 ℃ with distilled water, collects extracting solution, and is centrifugal, collect supernatant, with supernatant concentration, add ethanol, 4 ℃ of placements are spent the night, centrifugal, collecting precipitation is used respectively ethanol, acetone, ether washing precipitation, lyophilization, and get final product;
(4) preparation of Chinese yam polysaccharide
Above-mentioned Rhizoma Dioscoreae ethanol extraction is added the water redissolution be made into 5% solution, add Sevag reagent, concussion, centrifugal, get supernatant, add Sevag reagent, so repeatedly, until without white intermediate layer precipitation, collect supernatant concentration, use respectively tap water and distill water dialysis, add ethanol, 4 ℃ of placements are spent the night, centrifugal, collecting precipitation is used respectively ethanol, acetone and ether washing precipitation, lyophilization, and get final product;
(5) choosing in proportion Radix Scutellariae Rhizoma Dioscoreae water boiling concentration extract, baicalin, Rhizoma Dioscoreae ethanol extraction evenly mixes with Chinese yam polysaccharide, and get final product.
4. the preparation method of Chinese medicine extract as claimed in claim 3 characterized by further comprising: the product that step (5) makes is further made capsule or the tablet that contains Chinese medicine extract 100mg or 200mg specification by the pharmacy means of routine.
5. such as the purposes of each described Chinese medicine extract of claim 1-2 in preparing the medicine that reduces the reaction of cancer chemotherapy induced diarrhea and the treatment cancer is played potentiation.
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|---|---|---|---|---|
| CN1709334A (en) * | 2005-06-14 | 2005-12-21 | 胡锦国 | Shuanghuan anti-inflammation dispersion tablet of radix berberidis and radix scutellariae and its preparing method |
| CN101461910A (en) * | 2007-12-18 | 2009-06-24 | 丁月芳 | Chinese medicine spleen-strengthening powder for treating infantile diarrhea |
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2013
- 2013-06-24 CN CN2013102525029A patent/CN103316187A/en active Pending
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1709334A (en) * | 2005-06-14 | 2005-12-21 | 胡锦国 | Shuanghuan anti-inflammation dispersion tablet of radix berberidis and radix scutellariae and its preparing method |
| CN101461910A (en) * | 2007-12-18 | 2009-06-24 | 丁月芳 | Chinese medicine spleen-strengthening powder for treating infantile diarrhea |
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Application publication date: 20130925 |