CN103304330B - Culture medium and cultivation method of hericium erinaceus - Google Patents
Culture medium and cultivation method of hericium erinaceus Download PDFInfo
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- CN103304330B CN103304330B CN201310267883.8A CN201310267883A CN103304330B CN 103304330 B CN103304330 B CN 103304330B CN 201310267883 A CN201310267883 A CN 201310267883A CN 103304330 B CN103304330 B CN 103304330B
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Abstract
The invention relates to a culture medium and a cultivation method of hericium erinaceus. The cultivation method comprises the steps of: culture medium preparation, bagging, sterilization, inoculation, and germination management. The culture medium comprises the following raw materials: 30-35% of mulberry branches, 15-20% of corn cobs, 20-28% of cannabis sativa stems, 5-10% of cannabis sativa seeds, 5-10% of rice bran, 5-8% of corn meal, 1-3% of soybean meal, 2-3% of white sugar and 3-5% of cannabis sativa bran. With the adoption of the new culture medium formula and the cultivation method, the produced hericium erinaceus is high in yield, good in taste and high in medicinal property.
Description
Technical field
The present invention relates to fungus growing technique, particularly relate to substratum and the cultivating method of a kind of Hericium erinaceus (Bull. Ex Fr.) Pers..
Technical background
Hericium erinaceus (Bull. Ex Fr.) Pers. meat is tender delicious, nutritious, moisture 10.2g, protein 26.3g, fatty 4.2g, carbohydrate 44.9g, robust fibre 6.4g, calcium 2mg, phosphorus 856mg, iron 18mg, VitB1 0.69mg, carotene 0.01mg, riboflavin 1.89mg in the dry Hericium erinaceus (Bull. Ex Fr.) Pers. of every 100g, Hericium erinaceus (Bull. Ex Fr.) Pers. also can the illness such as partner treatment anorexia and loose stool, gastric and duodenal ulcer, neurasthenia, esophagus cancer, cancer of the stomach, dizzy, impotence.Along with the development of modern science and technology and clinical trial prove repeatedly, Hericium erinaceus (Bull. Ex Fr.) Pers. pharmaceutical use is constantly excavating the intension made new advances, and the pharmaceutical use of Hericium erinaceus (Bull. Ex Fr.) Pers. is mainly manifested in the following aspects: 1, antiulcer agent and anti-inflammatory action; 2, antitumor action; 3, liver protection function; 4, anti-aging effects; 5, improve body's hypoxia tolerance, increase heart blood work output, accelerate body blood circulation; 6, the effect of blood sugar and blood fat is reduced.
So, in recent years for the characteristic of Hericium erinaceus (Bull. Ex Fr.) Pers., develop a large amount of Hericium erinaceus (Bull. Ex Fr.) Pers. health foodstuff series and medicine, dimensions of market increases, occur planting Hericium erinaceus (Bull. Ex Fr.) Pers. in enormous quantities, those skilled in the art also on affecting Hericium erinaceus (Bull. Ex Fr.) Pers. nutrition, the factor of quality and output is studied, and finds that the impact on Hericium erinaceus (Bull. Ex Fr.) Pers. nutrition, quality and output of different plantation formula materials and cultivating method is very large.
Therefore, for those skilled in the art, need the substratum and cultivating method of developing the most applicable a kind of Hericium erinaceus (Bull. Ex Fr.) Pers. badly.
Summary of the invention
The present invention seeks to overcome the deficiencies in the prior art, having invented a kind of new substratum and cultivating method, the Hericium erinaceus (Bull. Ex Fr.) Pers. output produced is large, and mouthfeel is good, and medical is strong.
For solving the problems of the technologies described above, present invention employs following technical scheme:
A kind of Hericium erinaceus (Bull. Ex Fr.) Pers. substratum, formula and the weight proportion of its raw material are as follows:
Ramulus Mori 30-35%, corn cob 15-20%, fiery waste of flax 20-28%, fiery numb seed 5-10%, rice bran 5-10%, Semen Maydis powder 5-8%, analysis for soybean powder 1-3%, white sugar 2-3%, fiery numb bran 3-5%;
Use a Hericium erinaceus culture method for above-mentioned substratum, this cultivating method comprises the following steps:
1) medium preparing:
A: major ingredient used all should ted 3-5 days under the sun;
B: by corn cob with 0.5% lime water soak 24 hours after rinse with clear water;
C: in heaps after the batching rapid stirring after above-mentioned steps process is even, and cover with plastics film and carry out water content inspection;
Water content inspection described in step 1 detects the 60-65% that the water content of substratum accounts for substratum total amount;
2) pack: step 1 is processed wild Oryza species put into 13-17cm × 27-37cm bacterium bag in, when charging 1/3, shake gently several under, substratum is compressed downwards, refills substratum, shake again, press again, until purseful, make bacterium bag consolidation tight, add the collar and plug tampon;
3) sterilizing:
E, dress bacterium bag are complete, immediately by packed for bacterium enter sterilizing kitchen;
F, enter that bag is complete opens maximum fire immediately, make temperature in stove rise to 100 DEG C, keep 12-14 hour, stewing 24 hours of fire extinguishing;
In step e, bacterium bag enters stove and should stack into a line a line and discharge from bottom to top, will stay 3-5cm gap between row and row between bag and bag;
4) inoculate: inoculate at inoculation tank after sterilizing, transfer room schedule of operation is as follows:
G, liming with 10% carry out spraying disinfection, when bacterium bag temperature is down to room temperature, bacterium bag are sent into transfer room;
H, enter transfer room before, staff will wash one's hands, change sterile unlined long gown, cap, change slippers, alcohol disinfecting both hands with 70-75%, the liming with 10% sterilization seed bottle outer wall, light spirit lamp, by flame disinfection inoculating tool, above spirit lamp flame, open seed bottle, remove old mycelia;
I, open and connect bacterium sack, with inoculating tool, bacterial classification is accessed rapidly in sack, then seal;
5) hair tube reason: after bacterium bag enters culturing room, stack bacterium bag, the 1-4 days of Jun Daichuru culturing room, can not stir, and room temperature should be transferred to 24 DEG C-26 DEG C, from the 5th day, room temperature is adjusted to less than 24 DEG C, and after the 16th day, room temperature controls at 20 DEG C-23 DEG C, humidity 60%;
The culturing room of Jun Dairu described in step 57 days checks mycelial growth situation and whether pollution microbes afterwards, and discovery living contaminants bacterium bag is removed immediately;
Stack bacterium bag described in step 5, get the discharge of " well " word bilayer during temperature height, between bag, 3-5cm space will be had; Multilayer discharge when temperature is low;
6) management of producing mushroom: bacterium bag enters mushroom canopy three-dimensional discharge pile height 8-12 layer, then opening, and every two-layer bacterium bag puts one deck bamboo pole, temperature is adjusted to 14-20 DEG C, humidity reaches 85-90%, maintenance bacterium Bag Material face is moistening, mushroom canopy ventilates, illumination 200-400Lux, and former base can be plucked after forming 10-12 days and adopt.
The present invention compared with prior art, has following characteristics and advantage:
Hericium erinaceus culture technology of the present invention, introduces fiery waste of flax, fiery numb seed, fiery numb bran in the medium, through the Hericium erinaceus (Bull. Ex Fr.) Pers. repetition test of the present inventor by output, find that Hericium erinaceus (Bull. Ex Fr.) Pers. taste is fresh and tender, delicious good to eat, for doing medicinal material, the property of medicine is played better; By cultivating method of the present invention, the quality of Hericium erinaceus (Bull. Ex Fr.) Pers. and output are all increased.
Embodiment
Further describe the present invention below in conjunction with embodiment, to make those skilled in the art can implement according to this with reference to specification sheets word, scope is not limited by embodiments of the present invention.
Embodiment 1:
A kind of Hericium erinaceus (Bull. Ex Fr.) Pers. substratum, formula and the weight proportion of its raw material are as follows:
Ramulus Mori 30%, corn cob 15%, fiery waste of flax 28%, fiery numb seed 5%, rice bran 10%, Semen Maydis powder 5%, analysis for soybean powder 1%, white sugar 2%, fiery numb bran 4%;
Use a Hericium erinaceus culture method for above-mentioned substratum, this cultivating method comprises the following steps:
1) medium preparing:
A: major ingredient used all should ted 3 days under the sun;
B: by corn cob with 0.5% lime water soak 24 hours after rinse with clear water;
C: in heaps after the batching rapid stirring after above-mentioned steps process is even, and cover with plastics film and carry out water content inspection;
Water content inspection described in step 1 is that the water content detecting substratum accounts for 60% of substratum total amount;
2) pack: step 1 is processed wild Oryza species put into 13cm × 27cm bacterium bag in, when charging 1/3, shake gently several under, substratum is compressed downwards, refills substratum, shake again, press again, until purseful, make bacterium bag consolidation tight, add the collar and plug tampon;
3) sterilizing:
E, dress bacterium bag are complete, immediately by packed for bacterium enter sterilizing kitchen;
F, enter that bag is complete opens maximum fire immediately, make temperature in stove rise to 100 DEG C, keep 12 hours, stewing 24 hours of fire extinguishing;
In step e, bacterium bag enters stove and should stack into a line a line and discharge from bottom to top, will stay 3cm gap between row and row between bag and bag;
4) inoculate: inoculate at inoculation tank after sterilizing, transfer room schedule of operation is as follows:
G, liming with 10% carry out spraying disinfection, when bacterium bag temperature is down to room temperature, bacterium bag are sent into transfer room;
H, enter transfer room before, staff will wash one's hands, change sterile unlined long gown, cap, change slippers, alcohol disinfecting both hands with 70%, the liming with 10% sterilization seed bottle outer wall, light spirit lamp, by flame disinfection inoculating tool, above spirit lamp flame, open seed bottle, remove old mycelia;
I, open and connect bacterium sack, with inoculating tool, bacterial classification is accessed rapidly in sack, then seal;
5) hair tube reason: after bacterium bag enters culturing room, stack bacterium bag, 3 days of Jun Daichuru culturing room, can not stir, room temperature should be transferred to 24 DEG C, from the 5th day, room temperature is adjusted to less than 24 DEG C, and after the 16th day, room temperature controls at 20 DEG C, humidity 60%;
The culturing room of Jun Dairu described in step 57 days checks mycelial growth situation and whether pollution microbes afterwards, and discovery living contaminants bacterium bag is removed immediately;
Stack bacterium bag described in step 5, get the discharge of " well " word bilayer during temperature height, between bag, 3cm space will be had; Multilayer discharge when temperature is low;
6) management of producing mushroom: it is high 8 layers that bacterium bag enters the three-dimensional discharge pile of mushroom canopy, then opening, and every two-layer bacterium bag puts one deck bamboo pole, temperature is adjusted to 15 DEG C, humidity reaches 85%, keeps that bacterium Bag Material face is moistening, mushroom canopy ventilates, and illumination 200Lux, can pluck after former base forms 12 days and adopt.
The present invention is by new culture medium prescription and cultivating method, and the Hericium erinaceus (Bull. Ex Fr.) Pers. output produced is large, and fresh and tender, medical is strong.
Embodiment 2
A kind of Hericium erinaceus (Bull. Ex Fr.) Pers. substratum, formula and the weight proportion of its raw material are as follows:
Ramulus Mori 35%, corn cob 15%, fiery waste of flax 20%, fiery numb seed 10%, rice bran 5%, Semen Maydis powder 8%, analysis for soybean powder 2%, white sugar 2%, fiery numb bran 3%;
Use a Hericium erinaceus culture method for above-mentioned substratum, this cultivating method comprises the following steps:
1) medium preparing:
A: major ingredient used all should ted 5 days under the sun;
B: by corn cob with 0.5% lime water soak 24 hours after rinse with clear water;
C: in heaps after the batching rapid stirring after above-mentioned steps process is even, and cover with plastics film and carry out water content inspection;
Water content inspection described in step 1 is that the water content detecting substratum accounts for 65% of substratum total amount;
2) pack: step 1 is processed wild Oryza species put into 17cm × 37cm bacterium bag in, when charging 1/3, shake gently several under, substratum is compressed downwards, refills substratum, shake again, press again, until purseful, make bacterium bag consolidation tight, add the collar and plug tampon;
3) sterilizing:
E, dress bacterium bag are complete, immediately by packed for bacterium enter sterilizing kitchen;
F, enter that bag is complete opens maximum fire immediately, make temperature in stove rise to 100 DEG C, keep 14 hours, stewing 24 hours of fire extinguishing;
In step e, bacterium bag enters stove and should stack into a line a line and discharge from bottom to top, will stay 3-5cm gap between row and row between bag and bag;
4) inoculate: inoculate at inoculation tank after sterilizing, transfer room schedule of operation is as follows:
G, liming with 10% carry out spraying disinfection, when bacterium bag temperature is down to room temperature, bacterium bag are sent into transfer room;
H, enter transfer room before, staff will wash one's hands, change sterile unlined long gown, cap, change slippers, alcohol disinfecting both hands with 75%, the liming with 10% sterilization seed bottle outer wall, light spirit lamp, by flame disinfection inoculating tool, above spirit lamp flame, open seed bottle, remove old mycelia;
I, open and connect bacterium sack, with inoculating tool, bacterial classification is accessed rapidly in sack, then seal;
5) hair tube reason: after bacterium bag enters culturing room, stack bacterium bag, 4 days of Jun Daichuru culturing room, can not stir, room temperature should be transferred to 26 DEG C, from the 5th day, room temperature is adjusted to less than 24 DEG C, and after the 16th day, room temperature controls at 23 DEG C, humidity 60%;
The culturing room of Jun Dairu described in step 57 days checks mycelial growth situation and whether pollution microbes afterwards, and discovery living contaminants bacterium bag is removed immediately;
Stack bacterium bag described in step 5, get the discharge of " well " word bilayer during temperature height, between bag, 5cm space will be had; Multilayer discharge when temperature is low;
6) management of producing mushroom: it is high 12 layers that bacterium bag enters the three-dimensional discharge pile of mushroom canopy, then opening, and every two-layer bacterium bag puts one deck bamboo pole, temperature is adjusted to 20 DEG C, humidity reaches 90%, keeps that bacterium Bag Material face is moistening, mushroom canopy ventilates, and illumination 400Lux, can pluck after former base forms 12 days and adopt.
The present invention is by new culture medium prescription and cultivating method, and the Hericium erinaceus (Bull. Ex Fr.) Pers. output produced is large, fresh and tender and with crisp sense, medical is strong.
Claims (6)
1. a Hericium erinaceus (Bull. Ex Fr.) Pers. substratum, it is characterized in that: formula and the weight proportion of its raw material are as follows: Ramulus Mori 30-35%, corn cob 15-20%, fiery waste of flax 20-28%, fiery numb seed 5-10%, rice bran 5-10%, Semen Maydis powder 5-8%, analysis for soybean powder 1-3%, white sugar 2-3%, fiery numb bran 3-5%.
2. use a Hericium erinaceus culture method for Hericium erinaceus (Bull. Ex Fr.) Pers. substratum described in claim 1, it is characterized in that: this cultivating method comprises the following steps:
1) medium preparing:
A: major ingredient used all should ted 3-5 days under the sun;
B: by corn cob with 0.5% lime water soak 24 hours after rinse with clear water;
C: in heaps after the batching after above-mentioned steps process is stirred, and cover with plastics film and carry out water content inspection;
2) pack: by step 1) process wild Oryza species put into 13-17cm × 27-37cm bacterium bag in, when charging 1/3, shake gently several under, substratum is compressed downwards, refills substratum, shake again, press again, until purseful, make bacterium bag consolidation tight, add the collar and plug tampon;
3) sterilizing: dress bacterium bag is complete, by packed for bacterium enter sterilizing kitchen, make temperature in stove rise to 100 DEG C, keep 12-14 hour, stewing 24 hours of fire extinguishing;
4) inoculate:
G, liming with 10% carry out transfer room spraying disinfection, when bacterium bag temperature is down to room temperature, bacterium bag are sent into transfer room;
H, liming sterilization seed bottle outer wall with 10%, light spirit lamp, by flame disinfection inoculating tool, above spirit lamp flame, open seed bottle, remove old mycelia;
I, open and connect bacterium sack, with inoculating tool, bacterial classification is accessed in sack, then seal;
5) hair tube reason: after bacterium bag enters culturing room, stack bacterium bag, the 1-4 days of Jun Daichuru culturing room, can not stir, room temperature should be transferred to 24 DEG C-26 DEG C, from the 5th day, room temperature is adjusted to less than 24 DEG C, after the 16th day, room temperature controls at 20 DEG C-23 DEG C, humidity 60%;
6) management of producing mushroom: bacterium bag enters mushroom canopy three-dimensional discharge pile height 8-12 layer, then opening, and every two-layer bacterium bag puts one deck bamboo pole, temperature is adjusted to 14-20 DEG C, humidity reaches 85-90%, maintenance bacterium Bag Material face is moistening, mushroom canopy ventilates, illumination 200-400Lux, and former base can be plucked after forming 10-12 days and adopt.
3. Hericium erinaceus culture method according to claim 2, is characterized in that: step 1) described water content inspection is the 60-65% that the water content detecting substratum accounts for substratum total amount.
4. Hericium erinaceus culture method according to claim 2, is characterized in that: step 3) bacterium bag enters stove and should stack into a line a line and discharge from bottom to top in described sterilization steps, will stay 3-5cm gap between row and row between bag and bag.
5. Hericium erinaceus culture method according to claim 2, is characterized in that: described step 4) before staff enters transfer room, wash one's hands, change sterile unlined long gown, cap, change slippers, with the alcohol disinfecting both hands of 70-75%.
6. Hericium erinaceus culture method according to claim 2, is characterized in that: described step 5) described in Jun Dairu culturing room within 7 days, find that living contaminants bacterium bag will be removed afterwards.
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Families Citing this family (11)
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| CN103304333B (en) * | 2013-07-02 | 2015-06-24 | 广西巴马原生长寿食品有限公司 | Culture medium for lucid ganoderma and culture method |
| CN103772048B (en) * | 2014-01-27 | 2016-05-04 | 韦承梭 | The culture medium of a kind of coprinus comatus and cultural method |
| CN104429600A (en) * | 2014-11-25 | 2015-03-25 | 刘雄 | Lentinula edodes cultivation method |
| CN104737784A (en) * | 2015-03-13 | 2015-07-01 | 邬方成 | Cultivation method for hericium erinaceus |
| CN105165398A (en) * | 2015-09-15 | 2015-12-23 | 上海雪榕生物科技股份有限公司 | Mature material cultivation method for agaricus bisporus |
| CN105746176A (en) * | 2016-05-12 | 2016-07-13 | 贵州省农作物品种资源研究所 | Method for cultivating dictyophora rubrovolvata through cannabis stalk fermented feed fungus bed |
| CN107691110A (en) * | 2017-11-28 | 2018-02-16 | 南宁市生润科技有限公司 | The breeding method of monkey mushroom bacterium |
| CN109122028A (en) * | 2018-09-30 | 2019-01-04 | 安徽神州生态农业发展有限公司 | A kind of planting technique of Hericium erinaceus |
| CN114532151A (en) * | 2018-11-30 | 2022-05-27 | 成都市农林科学院 | Method for fermenting and culturing hericium erinaceus mycoplasm and application of hericium erinaceus mycoplasm |
| CN112088719A (en) * | 2020-09-10 | 2020-12-18 | 砀山野居农作物种植专业合作社 | Method for culturing edible fungi |
| CN112602530A (en) * | 2020-12-02 | 2021-04-06 | 镇江市菇满园生态农业有限公司 | Bag-cultivated hericium erinaceus intelligent control cultivation method |
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| CN1528117A (en) * | 2003-10-21 | 2004-09-15 | 中国科学院植物研究所 | Method for producing hedgehog fungus and special culture medium thereof |
| CN101683046A (en) * | 2008-09-22 | 2010-03-31 | 赵伟 | Method for preparing female seed of hericium erinaceum |
| CN102599004A (en) * | 2011-01-20 | 2012-07-25 | 云南云百草实验室有限公司 | Method for culturing Cordyceps Sinensis mycelia with hemp seed-containing culture medium |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1528117A (en) * | 2003-10-21 | 2004-09-15 | 中国科学院植物研究所 | Method for producing hedgehog fungus and special culture medium thereof |
| CN101683046A (en) * | 2008-09-22 | 2010-03-31 | 赵伟 | Method for preparing female seed of hericium erinaceum |
| CN102599004A (en) * | 2011-01-20 | 2012-07-25 | 云南云百草实验室有限公司 | Method for culturing Cordyceps Sinensis mycelia with hemp seed-containing culture medium |
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