CN103288975A - Extracting method for refining astragalus polysaccharide - Google Patents
Extracting method for refining astragalus polysaccharide Download PDFInfo
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- CN103288975A CN103288975A CN2013101737913A CN201310173791A CN103288975A CN 103288975 A CN103288975 A CN 103288975A CN 2013101737913 A CN2013101737913 A CN 2013101737913A CN 201310173791 A CN201310173791 A CN 201310173791A CN 103288975 A CN103288975 A CN 103288975A
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Abstract
The invention discloses an extracting method for refining astragalus polysaccharide. The extracting method comprises the following steps of: adding ethanol into astragalus membranaceus to carry out reflux degreasing, filtering the resulting product by using a micro-filtration membrane, and preserving the preliminary filtrating solution and the filter residue for later use; adding water into the filter residue to carry out reflux extraction, filtering the resulting product, preserving the secondary filtrating solution, mixing the filtrating solutions obtained from the two times, and condensing the mixed filtering solution by using an ultrafiltration membrane to obtain a concentrated solution; adding the ethanol with the volume fraction of 95% into the concentrated solution, stirring the mixed ethanol and the concentrated solution, centrifuging the stirred solution, and collecting the precipitate to obtain crude astragalus polysaccharide; adding water into the crude astragalus polysaccharide, adding an enzyme preparation into the aqueous solution of the astragalus polysaccharide, thus obtaining supernate; and adding cellulose into the supernate, filtering the resulting product to obtain a filtrating solution, condensing the filtrating solution, adding ethanol with the volume fraction of 80% into the concentrated solution, carrying out precipitation and centrifugation, and collecting the precipitate to obtain refined astragalus polysaccharide. The technologies of microfiltration, ultrafiltration, alcohol extraction and enzyme solution are applied in combination, the extraction efficiency of the astragalus polysaccharide is greatly improved, product purity is high, and the membrane separation is a physical separation process and does not need heating so that polysaccharide decomposition is prevented.
Description
Technical field
The present invention relates to a kind of extracting method of vegetable polysaccharides, relate in particular to a kind of extracting method of refining astragalus polysaccharide.
Background technology
Radix Astragali flavor is sweet, and slightly warm in nature is returned liver, spleen, lung, kidney channel, the effect that has benefiting QI for strengthening the superficies, arrest sweating to take off, hold in the palm sore myogenic, inducing diuresis to remove edema admittedly, and astragalus polysaccharides is one of the most effective composition in the Radix Astragali.Extract polysaccharide from the Radix Astragali, the water extraction extraction efficiency is low, and the step complexity, length consuming time, and loss of effective components is many; Disclosed a kind of extraction of Astragalus Polysaccharides in Astragalus method adopts refluxing extraction, flocculation, ultrafiltration, mistake resin column technology separation and Extraction astragalus polysaccharides, and the complex steps complexity, purity is low.Therefore, need provide a kind of new technical scheme to solve the problems referred to above.
Summary of the invention
The extracting method that the purpose of this invention is to provide the refining astragalus polysaccharide that a kind of technology is simple, extraction efficiency is high.
The technical solution used in the present invention:
A kind of extracting method of refining astragalus polysaccharide may further comprise the steps:
Add the ethanol degreasing that refluxes in a, the Radix Astragali, return time 2-3 hour, behind the micro-filtrate membrane filtration, keep first-time filtrate, filter residue is stand-by;
The water that the 5-8 of adding filter residue quality doubly measures in b, the filter residue carried out refluxing extraction 1-1.5 hour, filtered, and kept secondary filtrate, merged filtrate twice, concentrated with ultra-filtration membrane again, was concentrated into the 1/10-1/5 of filtrate volume, got concentrated solution;
C, to add volume fraction in concentrated solution be 95% ethanol, stirs, centrifugal after, the collecting precipitation thing is pulverized, after the drying, is got the crude product astragalus polysaccharides;
Add crude product astragalus polysaccharides quality 3-5 water doubly in d, the crude product astragalus polysaccharides, become the crude product astragalus polysaccharides aqueous solution, again according to the ratio of the mol ratio 1:1-5 of crude product astragalus polysaccharides and zymin, in the astragalus polysaccharides aqueous solution, add zymin, enzymolysis 3-5 hour, hydrolysis temperature 35-40 ℃, centrifugal, get supernatant liquor;
Add Mierocrystalline cellulose in e, the supernatant liquor, boiled 10-15 minute, filter, obtain filtrate, filtrate is concentrated into 1/10 of filtrate volume, adds the ethanol of volume fraction 80% in the concentrated solution, precipitation, centrifugal, behind the collecting precipitation thing, 60-80 ℃ of vacuum-drying gets the refining astragalus polysaccharide.
The mass ratio of the Radix Astragali and ethanol is 1:2-3 in a step.
Zymin is a kind of in cellulase, hemicellulase or the proteolytic enzyme.
Advantage of the present invention is: adopts micro-filtration, ultrafiltration, alcohol extracting and enzymolysis combined utilization technology, improved the extraction of Astragalus Polysaccharides in Astragalus rate greatly, and the product purity height, membrane sepn is physical separating process, need not heating, has avoided the polysaccharide decomposition.
Embodiment
Following embodiment only is used for explanation the present invention, but can not limit protection scope of the present invention.
The technical solution used in the present invention:
Embodiment 1
A kind of extracting method of refining astragalus polysaccharide may further comprise the steps:
Add the ethanol degreasing that refluxes in a, the Radix Astragali, the mass ratio of the Radix Astragali and ethanol is 1:2, return time 2 hours, and behind the micro-filtrate membrane filtration, the reservation first-time filtrate, filter residue is stand-by;
The water that adds 5 times of amounts of filter residue quality in b, the filter residue carried out refluxing extraction 1 hour, filtered, and kept secondary filtrate, merged filtrate twice, concentrated with ultra-filtration membrane again, was concentrated into 1/10 of filtrate volume, got concentrated solution;
C, to add volume fraction in concentrated solution be 95% ethanol, stirs, centrifugal after, the collecting precipitation thing is pulverized, after the drying, is got the crude product astragalus polysaccharides;
The water that adds 3 times of crude product astragalus polysaccharides quality in d, the crude product astragalus polysaccharides, become the crude product astragalus polysaccharides aqueous solution, again according to the ratio of the mol ratio 1:1 of crude product astragalus polysaccharides and zymin, in the astragalus polysaccharides aqueous solution, add zymin, zymin is cellulase, enzymolysis 3 hours, 35 ℃ of hydrolysis temperatures, centrifugal, get supernatant liquor;
Add Mierocrystalline cellulose in e, the supernatant liquor, boiled 10 minutes, filter, obtain filtrate, filtrate is concentrated into 1/10 of filtrate volume, add the ethanol of volume fraction 80% in the concentrated solution, precipitation, centrifugal, behind the collecting precipitation thing, 60 ℃ of vacuum-dryings get the refining astragalus polysaccharide.
Embodiment 2
A kind of extracting method of refining astragalus polysaccharide may further comprise the steps:
Add the ethanol degreasing that refluxes in a, the Radix Astragali, the mass ratio of the Radix Astragali and ethanol is 1:2.5, return time 2.5 hours, and behind the micro-filtrate membrane filtration, the reservation first-time filtrate, filter residue is stand-by;
The water that adds 6 times of amounts of filter residue quality in b, the filter residue carried out refluxing extraction 1.2 hours, filtered, and kept secondary filtrate, merged filtrate twice, concentrated with ultra-filtration membrane again, was concentrated into 1/8 of filtrate volume, got concentrated solution;
C, to add volume fraction in concentrated solution be 95% ethanol, stirs, centrifugal after, the collecting precipitation thing is pulverized, after the drying, is got the crude product astragalus polysaccharides;
The water that adds 4 times of crude product astragalus polysaccharides quality in d, the crude product astragalus polysaccharides, become the crude product astragalus polysaccharides aqueous solution, again according to the ratio of the mol ratio 1:3 of crude product astragalus polysaccharides and zymin, in the astragalus polysaccharides aqueous solution, add zymin, zymin is hemicellulase, enzymolysis 4 hours, 37 ℃ of hydrolysis temperatures, centrifugal, get supernatant liquor;
Add Mierocrystalline cellulose in e, the supernatant liquor, boiled 12 minutes, filter, obtain filtrate, filtrate is concentrated into 1/10 of filtrate volume, add the ethanol of volume fraction 80% in the concentrated solution, precipitation, centrifugal, behind the collecting precipitation thing, 70 ℃ of vacuum-dryings get the refining astragalus polysaccharide.
Embodiment 3
A kind of extracting method of refining astragalus polysaccharide may further comprise the steps:
Add the ethanol degreasing that refluxes in a, the Radix Astragali, the mass ratio of the Radix Astragali and ethanol is 1:3, return time 3 hours, and behind the micro-filtrate membrane filtration, the reservation first-time filtrate, filter residue is stand-by;
The water that adds 8 times of amounts of filter residue quality in b, the filter residue carried out refluxing extraction 1.5 hours, filtered, and kept secondary filtrate, merged filtrate twice, concentrated with ultra-filtration membrane again, was concentrated into 1/5 of filtrate volume, got concentrated solution;
C, to add volume fraction in concentrated solution be 95% ethanol, stirs, centrifugal after, the collecting precipitation thing is pulverized, after the drying, is got the crude product astragalus polysaccharides;
The water that adds 5 times of crude product astragalus polysaccharides quality in d, the crude product astragalus polysaccharides, become the crude product astragalus polysaccharides aqueous solution, again according to the ratio of the mol ratio 1:5 of crude product astragalus polysaccharides and zymin, in the astragalus polysaccharides aqueous solution, add zymin, zymin is proteolytic enzyme, enzymolysis 5 hours, 40 ℃ of hydrolysis temperatures, centrifugal, get supernatant liquor;
Add Mierocrystalline cellulose in e, the supernatant liquor, boiled 15 minutes, filter, obtain filtrate, filtrate is concentrated into 1/10 of filtrate volume, add the ethanol of volume fraction 80% in the concentrated solution, precipitation, centrifugal, behind the collecting precipitation thing, 80 ℃ of vacuum-dryings get the refining astragalus polysaccharide.
Add the ethanol degreasing that refluxes in the Radix Astragali, return time 3 hours, behind the micro-filtrate membrane filtration, keep first-time filtrate, filter residue is stand-by, and filter residue is further extracted, after filter residue carries out the water backflow, get secondary filtrate, behind the merging filtrate, carry out ultrafiltration, the adding volume fraction is 95% ethanol in the concentrated solution, obtain crude product, further purify to crude product, add zymin, zymin have mild condition, easily remove impurity, keep the advantage of astragalus polysaccharides activity, behind the enzymolysis, get supernatant liquor and further concentrate and alcohol extracting, get the refining astragalus polysaccharide at last.
Adopt micro-filtration, ultrafiltration, alcohol extracting and enzymolysis combined utilization technology, improved the extraction of Astragalus Polysaccharides in Astragalus rate greatly, the product purity height, membrane sepn is physical separating process, need not heating, has avoided the polysaccharide decomposition.
Claims (3)
1. the extracting method of a refining astragalus polysaccharide is characterized in that, may further comprise the steps:
Add the ethanol degreasing that refluxes in a, the Radix Astragali, return time 2-3 hour, behind the micro-filtrate membrane filtration, keep first-time filtrate, filter residue is stand-by;
The water that the 5-8 of adding filter residue quality doubly measures in b, the filter residue carried out refluxing extraction 1-1.5 hour, filtered, and kept secondary filtrate, merged filtrate twice, concentrated with ultra-filtration membrane again, was concentrated into the 1/10-1/5 of filtrate volume, got concentrated solution;
C, to add volume fraction in concentrated solution be 95% ethanol, stirs, centrifugal after, the collecting precipitation thing is pulverized, after the drying, is got the crude product astragalus polysaccharides;
Add crude product astragalus polysaccharides quality 3-5 water doubly in d, the crude product astragalus polysaccharides, become the crude product astragalus polysaccharides aqueous solution, again according to the ratio of the mol ratio 1:1-5 of crude product astragalus polysaccharides and zymin, in the astragalus polysaccharides aqueous solution, add zymin, enzymolysis 3-5 hour, hydrolysis temperature 35-40 ℃, centrifugal, get supernatant liquor;
Add Mierocrystalline cellulose in e, the supernatant liquor, boiled 10-15 minute, filter, obtain filtrate, filtrate is concentrated into 1/10 of filtrate volume, adds the ethanol of volume fraction 80% in the concentrated solution, precipitation, centrifugal, behind the collecting precipitation thing, 60-80 ℃ of vacuum-drying gets the refining astragalus polysaccharide.
2. the extracting method of a kind of refining astragalus polysaccharide according to claim 1, it is characterized in that: the mass ratio of the Radix Astragali and ethanol is 1:2-3 in a step.
3. the extracting method of a kind of refining astragalus polysaccharide according to claim 1 is characterized in that: zymin is a kind of in cellulase, hemicellulase or the proteolytic enzyme.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103772523A (en) * | 2014-01-14 | 2014-05-07 | 武汉新国峰科技开发有限公司 | New technology for preparing poria polysaccharide extract through membrane separation and purification technology |
| CN104558224A (en) * | 2014-12-08 | 2015-04-29 | 向华 | Method for preparing astragalus polysaccharide and water reservoir gel from astragalus residues |
| WO2015154691A1 (en) * | 2014-04-09 | 2015-10-15 | Phytohealth Corporation | A composition comprising astragalus membranaceus extract for preventing/treating brain injury and its preparation method |
| CN105412209A (en) * | 2015-12-08 | 2016-03-23 | 广东艾希德药业有限公司 | Astragalus extract and preparation method thereof and application |
| CN106977620A (en) * | 2017-06-03 | 2017-07-25 | 河南中医药大学 | A kind of method of purification of astragalus polyose |
| CN108084288A (en) * | 2017-12-26 | 2018-05-29 | 浦江县美泽生物科技有限公司 | efficient astragalus polyose extracting method |
| CN108117606A (en) * | 2016-11-30 | 2018-06-05 | 广西大学 | A kind of extraction Onion Polysaccharide crude extract technique |
| CN110218266A (en) * | 2018-12-18 | 2019-09-10 | 权冉(银川)科技有限公司 | A kind of fructus lycii or astragalus polyose process for extracting |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103772523A (en) * | 2014-01-14 | 2014-05-07 | 武汉新国峰科技开发有限公司 | New technology for preparing poria polysaccharide extract through membrane separation and purification technology |
| CN103772523B (en) * | 2014-01-14 | 2016-02-10 | 武汉新国峰科技开发有限公司 | A kind of membrane separation and purification technology prepares Pachymose extract novel process |
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| CN105412209B (en) * | 2015-12-08 | 2020-10-02 | 广东艾希德药业有限公司 | Astragalus extract and preparation method and application thereof |
| CN108117606A (en) * | 2016-11-30 | 2018-06-05 | 广西大学 | A kind of extraction Onion Polysaccharide crude extract technique |
| CN106977620A (en) * | 2017-06-03 | 2017-07-25 | 河南中医药大学 | A kind of method of purification of astragalus polyose |
| CN108084288A (en) * | 2017-12-26 | 2018-05-29 | 浦江县美泽生物科技有限公司 | efficient astragalus polyose extracting method |
| CN110218266A (en) * | 2018-12-18 | 2019-09-10 | 权冉(银川)科技有限公司 | A kind of fructus lycii or astragalus polyose process for extracting |
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Application publication date: 20130911 |