CN103665178B - A kind of gastrodia elata polysaccharide extracting method - Google Patents
A kind of gastrodia elata polysaccharide extracting method Download PDFInfo
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- CN103665178B CN103665178B CN201310585026.2A CN201310585026A CN103665178B CN 103665178 B CN103665178 B CN 103665178B CN 201310585026 A CN201310585026 A CN 201310585026A CN 103665178 B CN103665178 B CN 103665178B
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- 150000004676 glycans Chemical class 0.000 title claims abstract description 36
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 36
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 36
- 241000305491 Gastrodia elata Species 0.000 title claims abstract description 26
- 238000000034 method Methods 0.000 title claims abstract description 15
- 239000000284 extract Substances 0.000 claims abstract description 21
- 238000000605 extraction Methods 0.000 claims abstract description 16
- 238000000108 ultra-filtration Methods 0.000 claims abstract description 16
- 239000012528 membrane Substances 0.000 claims abstract description 15
- PUQSUZTXKPLAPR-UJPOAAIJSA-N Gastrodin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC=C(CO)C=C1 PUQSUZTXKPLAPR-UJPOAAIJSA-N 0.000 claims abstract description 13
- 238000005520 cutting process Methods 0.000 claims abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000012530 fluid Substances 0.000 claims abstract description 11
- 239000007788 liquid Substances 0.000 claims description 31
- 238000010298 pulverizing process Methods 0.000 claims description 10
- 238000010438 heat treatment Methods 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 5
- 239000000706 filtrate Substances 0.000 claims description 5
- 239000002994 raw material Substances 0.000 abstract description 7
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 239000003814 drug Substances 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 4
- 235000013305 food Nutrition 0.000 abstract description 2
- 230000001681 protective effect Effects 0.000 abstract 1
- 238000010992 reflux Methods 0.000 abstract 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 7
- 238000001556 precipitation Methods 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 230000008901 benefit Effects 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 238000003809 water extraction Methods 0.000 description 3
- 230000002082 anti-convulsion Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 244000025254 Cannabis sativa Species 0.000 description 1
- 206010007247 Carbuncle Diseases 0.000 description 1
- 206010010904 Convulsion Diseases 0.000 description 1
- 241001516470 Cypripedium acaule Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000305492 Gastrodia Species 0.000 description 1
- 241000045476 Gastrodieae Species 0.000 description 1
- 206010019233 Headaches Diseases 0.000 description 1
- 206010062717 Increased upper airway secretion Diseases 0.000 description 1
- 241000233855 Orchidaceae Species 0.000 description 1
- 208000002193 Pain Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000036592 analgesia Effects 0.000 description 1
- 230000000202 analgesic effect Effects 0.000 description 1
- 230000003556 anti-epileptic effect Effects 0.000 description 1
- 230000001663 anti-spastic effect Effects 0.000 description 1
- 239000001961 anticonvulsive agent Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 230000003177 cardiotonic effect Effects 0.000 description 1
- 210000000748 cardiovascular system Anatomy 0.000 description 1
- 210000003169 central nervous system Anatomy 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000036461 convulsion Effects 0.000 description 1
- 238000011026 diafiltration Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 208000002173 dizziness Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000013467 fragmentation Methods 0.000 description 1
- 238000006062 fragmentation reaction Methods 0.000 description 1
- 231100000869 headache Toxicity 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
- 230000002519 immonomodulatory effect Effects 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000002366 mineral element Substances 0.000 description 1
- 231100000862 numbness Toxicity 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000036407 pain Effects 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 208000026435 phlegm Diseases 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000012207 quantitative assay Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 239000001117 sulphuric acid Substances 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 229940126680 traditional chinese medicines Drugs 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
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- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to a kind of rhizoma Gastrodiae preparing Gastrodine medicinal extract with pharmaceutical factory, to extract residue be that raw material extracts the method for gastrodia elata polysaccharide.Technical scheme of the present invention is that rhizoma Gastrodiae Gastrodine medicinal extract being prepared by pharmaceutical factory extracts residue, in microwave reactor, uses water refluxing extraction, vat liquor first adopts molecule cutting quantity 150 ~ 120KDa ultra-filtration membrane to carry out uf processing, filtered solution continues to adopt the ultra-filtration membrane of molecule cutting quantity 20 ~ 10KDa to carry out uf processing, and trapped fluid lyophilize obtains gastrodia elata polysaccharide.Adopt the ultrafiltration of microwave-assisted ultra-filtration membrane to extract gastrodia elata polysaccharide, both improve the yield of polysaccharide, and turn improved the purity of polysaccharide, and also maintained the activity of polysaccharide.Have production technique simple, the operating time is short, is easy to the feature of suitability for industrialized production, and the gastrodia elata polysaccharide of production can be widely used in various protective foods and medicine.
Description
Technical field
The present invention relates to a kind of rhizoma Gastrodiae preparing Gastrodine medicinal extract with pharmaceutical factory, to extract residue be that raw material extracts the method for gastrodia elata polysaccharide, belongs to vegetable active composition and extract manufacture field.
Background technology
Rhizoma Gastrodiae (GastrodiaelataBl) belongs to the orchid family (Orehidaceae), the perennial heterotrophism herbaceous plant of Gastrodia (Gastrodieae).Have another name called DINGFENGCAO, clutch grass, celestial being's pin, yellow moccasin flower, rhizoma gastrodiae etc., rhizoma Gastrodiae is the rare Chinese medicine commonly used of doctor at all times, is also green health treasure, the applicating history of existing two thousand years so far.Rhizoma Gastrodiae is good to treat the disease such as dizziness and headache, convulsions tic, numbness of the limbs, rheumatism, body pain, language is unsuccessful, blood vessels are obstructed, phlegm carbuncle vapour lock, therefore have " three towns ", " three resist ", the saying of " one mends ", i.e. anti-epileptic, anticonvulsion, rheumatism, calmness, antispastic, analgesia, qi-restoratives.Modern pharmacological research shows, rhizoma Gastrodiae mainly contains four large therapeutic actions: to calmness, the anticonvulsion and analgesic activity of central nervous system; To the cardiotonic of cardiovascular systems; There is the effect of resisting oxygen lack and enhancing immunologic function; Meanwhile, rhizoma Gastrodiae also has good health-care effect.The principle active component of rhizoma Gastrodiae is the phenoloids such as Gastrodine and gastrodia elata polysaccharide, wherein gastrodia elata polysaccharide have immunomodulatory, hypoglycemic, remove interior free yl, the effect such as delay senility.Separately have multiple mineral element and protein, amino acid, but the research of modern science to rhizoma Gastrodiae mainly concentrates on Gastrodine, and for other composition contained in rhizoma Gastrodiae, particularly gastrodia elata polysaccharide research is less, both system was not deep enough yet not.In existing patent documentation, only there is CN101357192B with the method for separating and preparing of the fresh rhizoma Gastrodiae rhizoma Gastrodiae Crude polysaccharides that is raw material and rhizoma Gastrodiae alcohol soluble substance, fresh rhizoma Gastrodiae completes, fragmentation, the defibrination that adds water, slurries filter, clear liquid alcohol precipitation, obtain rhizoma Gastrodiae Crude polysaccharides, the alcohol diafiltration of rhizoma Gastrodiae filter residue is extracted, extracting solution through concentrated, dry, rhizoma Gastrodiae alcohol soluble substance.Its rhizoma Gastrodiae Crude polysaccharides contains protein, protein bound sugar, oligose etc.In non-patent literature: [brightly to build. the separation and purification of rhizoma Gastrodiae water-soluble polysaccharide and physico-chemical property research. Food science, 2008,29 (9): 344-348; Zhao Peng. the research overview of gastrodia elata polysaccharide. Gansu Chinese of Traditional Chinese Medicine's journal, 2008,25 (4): 49-52; Zhu Xiaoxia etc. Extraction Parameters for Polysaccharides of Gastrodia elata Bl optimization. time precious traditional Chinese medicines medicine, 2007,18(4): 906-907; Liu Mingqing etc. Extraction Parameters for Polysaccharides of Gastrodia elata Bl and purifying research. Chinese pharmacists .2011,14 (11): 1593-1596] all with dry rhizoma Gastrodiae for raw material, water extraction, Seaveg method removing protein, alcohol precipitation etc. prepare gastrodia elata polysaccharide; Have no and prepare the rhizoma Gastrodiae extraction residue of Gastrodine medicinal extract for raw material with pharmaceutical factory, membrane sepn prepares the report of gastrodia elata polysaccharide.
Summary of the invention
The invention provides a kind of rhizoma Gastrodiae preparing Gastrodine medicinal extract with pharmaceutical factory, to extract residue be that raw material extracts the method for gastrodia elata polysaccharide, take full advantage of secondary resource, by simple and feasible, can the strong method of action row, the problems such as the step of preparation process solving gastrodia elata polysaccharide is complicated, production cost is higher.
The technical solution used in the present invention: after the rhizoma Gastrodiae extraction residue of Gastrodine medicinal extract, drying, pulverizing being prepared by pharmaceutical factory, add 6 ~ 15 times of water, in microwave reactor, when microwave power 600 ~ 800W, heating extraction 5 ~ 12 minutes, filters through gallus-type whizzer, obtains feed liquid 1; Filter residue adds 5 ~ 10 times of water yields in microwave power 600 ~ 800W, then extracts 2 ~ 6 minutes, filters to obtain feed liquid 2; Merge feed liquid 1 and feed liquid 2, the feed liquid of merging is again through the filter of clear tubular-bowl centrifuge essence; Essence filtrate liquid adopts molecule cutting quantity 150 ~ 120KDa ultra-filtration membrane to be 0.08 ~ 0.20MPa at pressure, uf processing is carried out when temperature is 20 ~ 40 DEG C, filtered solution continues to adopt the ultra-filtration membrane of molecule cutting quantity 20 ~ 10KDa to be 0.08 ~ 0.25MPa at pressure, temperature is 20 ~ 40 DEG C and carries out uf processing, collects trapped fluid; Trapped fluid lyophilize, obtains gastrodia elata polysaccharide after pulverizing.
Advantage of the present invention is: with pharmaceutical factory prepare Gastrodine medicinal extract rhizoma Gastrodiae extract residue be raw material, the effective constituent taken full advantage of in rhizoma Gastrodiae, decrease the wasting of resources; Gastrodia elata polysaccharide is extracted in microwave-assisted ultra-filtration membrane ultrafiltration simultaneously, and the operating time is short, and the yield of gastrodia elata polysaccharide is greatly improved than traditional water extraction and alcohol precipitation method, and the content of the polysaccharide of acquisition, also higher than the product of water extraction and alcohol precipitation method, also maintains the structure and energy of polysaccharide; By phend-sulphuric acid quantitative assay, the polysaccharide content that present method is extracted is 55 ~ 62%.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail, but embodiment is not limitation of the invention.
Embodiment one
After the rhizoma Gastrodiae extraction residue drying of Gastrodine medicinal extract, pulverizing being prepared by pharmaceutical factory, add 15 times of water, in microwave reactor, when microwave power 600W, heating extraction 10 minutes, filter to obtain feed liquid 1, filter residue adds 8 times of water yields in microwave power 600W, then extracts 4 minutes, filters to obtain feed liquid 2, merge feed liquid 1 and feed liquid 2, the feed liquid of merging is through the filter of clear tubular-bowl centrifuge essence; Essence filtrate liquid adopts molecule cutting quantity 150KDa ultra-filtration membrane to be 0.10MPa at pressure, uf processing is carried out when temperature is 35 DEG C, filtered solution continues to adopt the ultra-filtration membrane of molecule cutting quantity 20KDa to be 0.09MPa at pressure, temperature is 30 DEG C and carries out uf processing, collect trapped fluid, trapped fluid lyophilize, obtains gastrodia elata polysaccharide after pulverizing.
Embodiment two
After the rhizoma Gastrodiae extraction residue drying of Gastrodine medicinal extract, pulverizing being prepared by pharmaceutical factory, add 12 times of water, in microwave reactor, when microwave power 800W, heating extraction 5 minutes, filter to obtain feed liquid 1, filter residue adds 6 times of water yields in microwave power 800W, then extracts 2 minutes, filters to obtain feed liquid 2, merge feed liquid 1 and feed liquid 2, the feed liquid of merging is through the filter of clear tubular-bowl centrifuge essence; Essence filtrate liquid adopts molecule cutting quantity 150KDa ultra-filtration membrane to be 0.15MPa at pressure, temperature is 30 DEG C, carry out uf processing, filtered solution continues to adopt the ultra-filtration membrane of molecule cutting quantity 20KDa to be 0.12MPa at pressure, temperature is 30 DEG C and carries out uf processing, collect trapped fluid, trapped fluid lyophilize, obtains gastrodia elata polysaccharide after pulverizing.
Embodiment three
After the rhizoma Gastrodiae extraction residue drying of Gastrodine medicinal extract, pulverizing being prepared by pharmaceutical factory, add 10 times of water, in microwave reactor, when microwave power 800W, heating extraction 6 minutes, filter to obtain feed liquid 1, filter residue adds 8 times of water yields in microwave power 600W, then extracts 4 minutes, filters to obtain feed liquid 2, merge feed liquid 1 and feed liquid 2, the feed liquid of merging is through the filter of clear tubular-bowl centrifuge essence; Essence filtrate liquid adopts molecule cutting quantity 120KDa ultra-filtration membrane to be 0.20MPa at pressure, temperature is 40 DEG C and carries out uf processing, filtered solution continues to adopt the ultra-filtration membrane of molecule cutting quantity 10KDa to be 0.15MPa at pressure, temperature is 40 DEG C and carries out uf processing, collect trapped fluid, trapped fluid lyophilize, obtains gastrodia elata polysaccharide after pulverizing.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention.All any amendments done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within protection scope of the present invention.
Claims (2)
1. a gastrodia elata polysaccharide extracting method, described method comprises:
(1), after being prepared by pharmaceutical factory the rhizoma Gastrodiae extraction residue of Gastrodine medicinal extract, drying, pulverizing, add 6 ~ 20 times of water, in microwave reactor, heating is extracted, and filters to obtain feed liquid 1; Filter residue adds 5 ~ 10 times of water, reheats extraction in microwave reactor, filters to obtain feed liquid 2; Merge feed liquid 1 and feed liquid 2, the feed liquid of merging is again through the filter of clear tubular-bowl centrifuge essence;
(2) smart filtrate liquid adopts molecule cutting quantity 150 ~ 120KDa ultra-filtration membrane to be 0.08 ~ 0.20MPa at pressure, temperature is 20 ~ 40 DEG C and carries out uf processing, filtered solution continues to adopt the ultra-filtration membrane of molecule cutting quantity 20 ~ 10KDa to be 0.08 ~ 0.25MPa at pressure, temperature is 20 ~ 40 DEG C and carries out uf processing, collects trapped fluid;
(3) trapped fluid lyophilize, obtains gastrodia elata polysaccharide after pulverizing.
2. the extracting method of a kind of gastrodia elata polysaccharide as claimed in claim 1, is characterized in that: during the extracting through microwave heating of step (1), microwave power 600 ~ 800W, 5 ~ 12 minutes extraction times of first time, 2 ~ 6 minutes extraction times of second time.
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| CN105859899A (en) * | 2016-04-10 | 2016-08-17 | 黄河水利职业技术学院 | Rhizoma gastrodiae polysaccharide extraction method |
| CN113754706A (en) * | 2021-09-10 | 2021-12-07 | 广东青云山药业有限公司 | Gastrodia elata extract and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102464725A (en) * | 2010-11-17 | 2012-05-23 | 中国科学院上海药物研究所 | Gastrodia elata BL. polysaccharide and degradation products thereof and preparation methods and applications of degradation products |
| CN103242461A (en) * | 2012-02-06 | 2013-08-14 | 陕西理工学院 | Novel method for simultaneously extracting gastrodia elata polysaccharide and gastrodine |
-
2013
- 2013-11-19 CN CN201310585026.2A patent/CN103665178B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102464725A (en) * | 2010-11-17 | 2012-05-23 | 中国科学院上海药物研究所 | Gastrodia elata BL. polysaccharide and degradation products thereof and preparation methods and applications of degradation products |
| CN103242461A (en) * | 2012-02-06 | 2013-08-14 | 陕西理工学院 | Novel method for simultaneously extracting gastrodia elata polysaccharide and gastrodine |
Non-Patent Citations (2)
| Title |
|---|
| "天麻提取物工艺及其功效成分含量的分析";金虹等;《中草药》;20090525;第32卷(第5期);第799-802页 * |
| "微波法提取天麻多糖的研究";李志英等;《山西大学学报（自然科学版）》;20081115;第31卷(第4期);第573-576页 * |
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Effective date of registration: 20231126 Address after: No. 101, Building 10, Wumenggu Town, Longquan Street, Zhaoyang District, Zhaotong City, Yunnan Province, 657099 Patentee after: Yunnan Bangming Tianma Biotechnology Co.,Ltd. Address before: Yunnan city of Kunming Province, Kunming University of Science and Technology lotus Huayuan 37-3-206 Patentee before: Liu Xiangyi |