CN103665178B - A kind of gastrodia elata polysaccharide extracting method - Google Patents

A kind of gastrodia elata polysaccharide extracting method Download PDF

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CN103665178B
CN103665178B CN201310585026.2A CN201310585026A CN103665178B CN 103665178 B CN103665178 B CN 103665178B CN 201310585026 A CN201310585026 A CN 201310585026A CN 103665178 B CN103665178 B CN 103665178B
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gastrodia elata
polysaccharide
feed liquid
ultra
rhizoma gastrodiae
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CN103665178A (en
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刘祥义
李雪梅
毕荣璐
刘镭
马仲军
赵声富
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Yunnan Bangming Tianma Biotechnology Co ltd
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Abstract

The present invention relates to a kind of rhizoma Gastrodiae preparing Gastrodine medicinal extract with pharmaceutical factory, to extract residue be that raw material extracts the method for gastrodia elata polysaccharide.Technical scheme of the present invention is that rhizoma Gastrodiae Gastrodine medicinal extract being prepared by pharmaceutical factory extracts residue, in microwave reactor, uses water refluxing extraction, vat liquor first adopts molecule cutting quantity 150 ~ 120KDa ultra-filtration membrane to carry out uf processing, filtered solution continues to adopt the ultra-filtration membrane of molecule cutting quantity 20 ~ 10KDa to carry out uf processing, and trapped fluid lyophilize obtains gastrodia elata polysaccharide.Adopt the ultrafiltration of microwave-assisted ultra-filtration membrane to extract gastrodia elata polysaccharide, both improve the yield of polysaccharide, and turn improved the purity of polysaccharide, and also maintained the activity of polysaccharide.Have production technique simple, the operating time is short, is easy to the feature of suitability for industrialized production, and the gastrodia elata polysaccharide of production can be widely used in various protective foods and medicine.

Description

A kind of gastrodia elata polysaccharide extracting method
Technical field
The present invention relates to a kind of rhizoma Gastrodiae preparing Gastrodine medicinal extract with pharmaceutical factory, to extract residue be that raw material extracts the method for gastrodia elata polysaccharide, belongs to vegetable active composition and extract manufacture field.
Background technology
Rhizoma Gastrodiae (GastrodiaelataBl) belongs to the orchid family (Orehidaceae), the perennial heterotrophism herbaceous plant of Gastrodia (Gastrodieae).Have another name called DINGFENGCAO, clutch grass, celestial being's pin, yellow moccasin flower, rhizoma gastrodiae etc., rhizoma Gastrodiae is the rare Chinese medicine commonly used of doctor at all times, is also green health treasure, the applicating history of existing two thousand years so far.Rhizoma Gastrodiae is good to treat the disease such as dizziness and headache, convulsions tic, numbness of the limbs, rheumatism, body pain, language is unsuccessful, blood vessels are obstructed, phlegm carbuncle vapour lock, therefore have " three towns ", " three resist ", the saying of " one mends ", i.e. anti-epileptic, anticonvulsion, rheumatism, calmness, antispastic, analgesia, qi-restoratives.Modern pharmacological research shows, rhizoma Gastrodiae mainly contains four large therapeutic actions: to calmness, the anticonvulsion and analgesic activity of central nervous system; To the cardiotonic of cardiovascular systems; There is the effect of resisting oxygen lack and enhancing immunologic function; Meanwhile, rhizoma Gastrodiae also has good health-care effect.The principle active component of rhizoma Gastrodiae is the phenoloids such as Gastrodine and gastrodia elata polysaccharide, wherein gastrodia elata polysaccharide have immunomodulatory, hypoglycemic, remove interior free yl, the effect such as delay senility.Separately have multiple mineral element and protein, amino acid, but the research of modern science to rhizoma Gastrodiae mainly concentrates on Gastrodine, and for other composition contained in rhizoma Gastrodiae, particularly gastrodia elata polysaccharide research is less, both system was not deep enough yet not.In existing patent documentation, only there is CN101357192B with the method for separating and preparing of the fresh rhizoma Gastrodiae rhizoma Gastrodiae Crude polysaccharides that is raw material and rhizoma Gastrodiae alcohol soluble substance, fresh rhizoma Gastrodiae completes, fragmentation, the defibrination that adds water, slurries filter, clear liquid alcohol precipitation, obtain rhizoma Gastrodiae Crude polysaccharides, the alcohol diafiltration of rhizoma Gastrodiae filter residue is extracted, extracting solution through concentrated, dry, rhizoma Gastrodiae alcohol soluble substance.Its rhizoma Gastrodiae Crude polysaccharides contains protein, protein bound sugar, oligose etc.In non-patent literature: [brightly to build. the separation and purification of rhizoma Gastrodiae water-soluble polysaccharide and physico-chemical property research. Food science, 2008,29 (9): 344-348; Zhao Peng. the research overview of gastrodia elata polysaccharide. Gansu Chinese of Traditional Chinese Medicine's journal, 2008,25 (4): 49-52; Zhu Xiaoxia etc. Extraction Parameters for Polysaccharides of Gastrodia elata Bl optimization. time precious traditional Chinese medicines medicine, 2007,18(4): 906-907; Liu Mingqing etc. Extraction Parameters for Polysaccharides of Gastrodia elata Bl and purifying research. Chinese pharmacists .2011,14 (11): 1593-1596] all with dry rhizoma Gastrodiae for raw material, water extraction, Seaveg method removing protein, alcohol precipitation etc. prepare gastrodia elata polysaccharide; Have no and prepare the rhizoma Gastrodiae extraction residue of Gastrodine medicinal extract for raw material with pharmaceutical factory, membrane sepn prepares the report of gastrodia elata polysaccharide.
Summary of the invention
The invention provides a kind of rhizoma Gastrodiae preparing Gastrodine medicinal extract with pharmaceutical factory, to extract residue be that raw material extracts the method for gastrodia elata polysaccharide, take full advantage of secondary resource, by simple and feasible, can the strong method of action row, the problems such as the step of preparation process solving gastrodia elata polysaccharide is complicated, production cost is higher.
The technical solution used in the present invention: after the rhizoma Gastrodiae extraction residue of Gastrodine medicinal extract, drying, pulverizing being prepared by pharmaceutical factory, add 6 ~ 15 times of water, in microwave reactor, when microwave power 600 ~ 800W, heating extraction 5 ~ 12 minutes, filters through gallus-type whizzer, obtains feed liquid 1; Filter residue adds 5 ~ 10 times of water yields in microwave power 600 ~ 800W, then extracts 2 ~ 6 minutes, filters to obtain feed liquid 2; Merge feed liquid 1 and feed liquid 2, the feed liquid of merging is again through the filter of clear tubular-bowl centrifuge essence; Essence filtrate liquid adopts molecule cutting quantity 150 ~ 120KDa ultra-filtration membrane to be 0.08 ~ 0.20MPa at pressure, uf processing is carried out when temperature is 20 ~ 40 DEG C, filtered solution continues to adopt the ultra-filtration membrane of molecule cutting quantity 20 ~ 10KDa to be 0.08 ~ 0.25MPa at pressure, temperature is 20 ~ 40 DEG C and carries out uf processing, collects trapped fluid; Trapped fluid lyophilize, obtains gastrodia elata polysaccharide after pulverizing.
Advantage of the present invention is: with pharmaceutical factory prepare Gastrodine medicinal extract rhizoma Gastrodiae extract residue be raw material, the effective constituent taken full advantage of in rhizoma Gastrodiae, decrease the wasting of resources; Gastrodia elata polysaccharide is extracted in microwave-assisted ultra-filtration membrane ultrafiltration simultaneously, and the operating time is short, and the yield of gastrodia elata polysaccharide is greatly improved than traditional water extraction and alcohol precipitation method, and the content of the polysaccharide of acquisition, also higher than the product of water extraction and alcohol precipitation method, also maintains the structure and energy of polysaccharide; By phend-sulphuric acid quantitative assay, the polysaccharide content that present method is extracted is 55 ~ 62%.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail, but embodiment is not limitation of the invention.
Embodiment one
After the rhizoma Gastrodiae extraction residue drying of Gastrodine medicinal extract, pulverizing being prepared by pharmaceutical factory, add 15 times of water, in microwave reactor, when microwave power 600W, heating extraction 10 minutes, filter to obtain feed liquid 1, filter residue adds 8 times of water yields in microwave power 600W, then extracts 4 minutes, filters to obtain feed liquid 2, merge feed liquid 1 and feed liquid 2, the feed liquid of merging is through the filter of clear tubular-bowl centrifuge essence; Essence filtrate liquid adopts molecule cutting quantity 150KDa ultra-filtration membrane to be 0.10MPa at pressure, uf processing is carried out when temperature is 35 DEG C, filtered solution continues to adopt the ultra-filtration membrane of molecule cutting quantity 20KDa to be 0.09MPa at pressure, temperature is 30 DEG C and carries out uf processing, collect trapped fluid, trapped fluid lyophilize, obtains gastrodia elata polysaccharide after pulverizing.
Embodiment two
After the rhizoma Gastrodiae extraction residue drying of Gastrodine medicinal extract, pulverizing being prepared by pharmaceutical factory, add 12 times of water, in microwave reactor, when microwave power 800W, heating extraction 5 minutes, filter to obtain feed liquid 1, filter residue adds 6 times of water yields in microwave power 800W, then extracts 2 minutes, filters to obtain feed liquid 2, merge feed liquid 1 and feed liquid 2, the feed liquid of merging is through the filter of clear tubular-bowl centrifuge essence; Essence filtrate liquid adopts molecule cutting quantity 150KDa ultra-filtration membrane to be 0.15MPa at pressure, temperature is 30 DEG C, carry out uf processing, filtered solution continues to adopt the ultra-filtration membrane of molecule cutting quantity 20KDa to be 0.12MPa at pressure, temperature is 30 DEG C and carries out uf processing, collect trapped fluid, trapped fluid lyophilize, obtains gastrodia elata polysaccharide after pulverizing.
Embodiment three
After the rhizoma Gastrodiae extraction residue drying of Gastrodine medicinal extract, pulverizing being prepared by pharmaceutical factory, add 10 times of water, in microwave reactor, when microwave power 800W, heating extraction 6 minutes, filter to obtain feed liquid 1, filter residue adds 8 times of water yields in microwave power 600W, then extracts 4 minutes, filters to obtain feed liquid 2, merge feed liquid 1 and feed liquid 2, the feed liquid of merging is through the filter of clear tubular-bowl centrifuge essence; Essence filtrate liquid adopts molecule cutting quantity 120KDa ultra-filtration membrane to be 0.20MPa at pressure, temperature is 40 DEG C and carries out uf processing, filtered solution continues to adopt the ultra-filtration membrane of molecule cutting quantity 10KDa to be 0.15MPa at pressure, temperature is 40 DEG C and carries out uf processing, collect trapped fluid, trapped fluid lyophilize, obtains gastrodia elata polysaccharide after pulverizing.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention.All any amendments done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within protection scope of the present invention.

Claims (2)

1. a gastrodia elata polysaccharide extracting method, described method comprises:
(1), after being prepared by pharmaceutical factory the rhizoma Gastrodiae extraction residue of Gastrodine medicinal extract, drying, pulverizing, add 6 ~ 20 times of water, in microwave reactor, heating is extracted, and filters to obtain feed liquid 1; Filter residue adds 5 ~ 10 times of water, reheats extraction in microwave reactor, filters to obtain feed liquid 2; Merge feed liquid 1 and feed liquid 2, the feed liquid of merging is again through the filter of clear tubular-bowl centrifuge essence;
(2) smart filtrate liquid adopts molecule cutting quantity 150 ~ 120KDa ultra-filtration membrane to be 0.08 ~ 0.20MPa at pressure, temperature is 20 ~ 40 DEG C and carries out uf processing, filtered solution continues to adopt the ultra-filtration membrane of molecule cutting quantity 20 ~ 10KDa to be 0.08 ~ 0.25MPa at pressure, temperature is 20 ~ 40 DEG C and carries out uf processing, collects trapped fluid;
(3) trapped fluid lyophilize, obtains gastrodia elata polysaccharide after pulverizing.
2. the extracting method of a kind of gastrodia elata polysaccharide as claimed in claim 1, is characterized in that: during the extracting through microwave heating of step (1), microwave power 600 ~ 800W, 5 ~ 12 minutes extraction times of first time, 2 ~ 6 minutes extraction times of second time.
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CN105859899A (en) * 2016-04-10 2016-08-17 黄河水利职业技术学院 Rhizoma gastrodiae polysaccharide extraction method
CN113754706A (en) * 2021-09-10 2021-12-07 广东青云山药业有限公司 Gastrodia elata extract and preparation method thereof

Citations (2)

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Publication number Priority date Publication date Assignee Title
CN102464725A (en) * 2010-11-17 2012-05-23 中国科学院上海药物研究所 Gastrodia elata BL. polysaccharide and degradation products thereof and preparation methods and applications of degradation products
CN103242461A (en) * 2012-02-06 2013-08-14 陕西理工学院 Novel method for simultaneously extracting gastrodia elata polysaccharide and gastrodine

Patent Citations (2)

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Publication number Priority date Publication date Assignee Title
CN102464725A (en) * 2010-11-17 2012-05-23 中国科学院上海药物研究所 Gastrodia elata BL. polysaccharide and degradation products thereof and preparation methods and applications of degradation products
CN103242461A (en) * 2012-02-06 2013-08-14 陕西理工学院 Novel method for simultaneously extracting gastrodia elata polysaccharide and gastrodine

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Patentee before: Liu Xiangyi