CN103665178A - Method for extracting gastrodia elata polysaccharide - Google Patents
Method for extracting gastrodia elata polysaccharide Download PDFInfo
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- CN103665178A CN103665178A CN201310585026.2A CN201310585026A CN103665178A CN 103665178 A CN103665178 A CN 103665178A CN 201310585026 A CN201310585026 A CN 201310585026A CN 103665178 A CN103665178 A CN 103665178A
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- gastrodia elata
- elata polysaccharide
- polysaccharide
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- 150000004676 glycans Chemical class 0.000 title claims abstract description 37
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 37
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 37
- 241000305491 Gastrodia elata Species 0.000 title claims abstract description 31
- 238000000034 method Methods 0.000 title claims abstract description 17
- 238000000108 ultra-filtration Methods 0.000 claims abstract description 16
- 239000012528 membrane Substances 0.000 claims abstract description 15
- PUQSUZTXKPLAPR-UJPOAAIJSA-N Gastrodin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC=C(CO)C=C1 PUQSUZTXKPLAPR-UJPOAAIJSA-N 0.000 claims abstract description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 12
- 238000005520 cutting process Methods 0.000 claims abstract description 11
- 239000012530 fluid Substances 0.000 claims abstract description 11
- 238000000605 extraction Methods 0.000 claims abstract description 6
- 239000007788 liquid Substances 0.000 claims description 31
- 239000000284 extract Substances 0.000 claims description 22
- 238000010438 heat treatment Methods 0.000 claims description 6
- 239000000706 filtrate Substances 0.000 claims description 5
- 238000010298 pulverizing process Methods 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims 2
- 239000003814 drug Substances 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 4
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 238000005516 engineering process Methods 0.000 abstract description 2
- PUQSUZTXKPLAPR-KSSYENDESA-N 4-(beta-D-Glucopyranosyloxy) benzyl alcohol Natural products O([C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1)c1ccc(CO)cc1 PUQSUZTXKPLAPR-KSSYENDESA-N 0.000 abstract 2
- 229930193974 gastrodin Natural products 0.000 abstract 2
- PUQSUZTXKPLAPR-NZEXEKPDSA-N helicidol Natural products O([C@H]1[C@H](O)[C@H](O)[C@@H](O)[C@@H](CO)O1)c1ccc(CO)cc1 PUQSUZTXKPLAPR-NZEXEKPDSA-N 0.000 abstract 2
- 229940079593 drug Drugs 0.000 abstract 1
- 238000001035 drying Methods 0.000 abstract 1
- 230000008014 freezing Effects 0.000 abstract 1
- 238000007710 freezing Methods 0.000 abstract 1
- 235000013402 health food Nutrition 0.000 abstract 1
- 238000002386 leaching Methods 0.000 abstract 1
- 238000010992 reflux Methods 0.000 abstract 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 7
- 239000002994 raw material Substances 0.000 description 6
- 238000001556 precipitation Methods 0.000 description 4
- 230000008901 benefit Effects 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 238000003809 water extraction Methods 0.000 description 3
- 230000002082 anti-convulsion Effects 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000002002 slurry Substances 0.000 description 2
- 244000025254 Cannabis sativa Species 0.000 description 1
- 206010007247 Carbuncle Diseases 0.000 description 1
- 206010010904 Convulsion Diseases 0.000 description 1
- 241001516470 Cypripedium acaule Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000305492 Gastrodia Species 0.000 description 1
- 241000045476 Gastrodieae Species 0.000 description 1
- 206010019233 Headaches Diseases 0.000 description 1
- 206010062717 Increased upper airway secretion Diseases 0.000 description 1
- 241000233855 Orchidaceae Species 0.000 description 1
- 208000002193 Pain Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000036592 analgesia Effects 0.000 description 1
- 230000000202 analgesic effect Effects 0.000 description 1
- 230000003556 anti-epileptic effect Effects 0.000 description 1
- 230000001663 anti-spastic effect Effects 0.000 description 1
- 239000001961 anticonvulsive agent Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 230000003177 cardiotonic effect Effects 0.000 description 1
- 210000000748 cardiovascular system Anatomy 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000036461 convulsion Effects 0.000 description 1
- 238000011026 diafiltration Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 208000002173 dizziness Diseases 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 231100000869 headache Toxicity 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
- 230000002519 immonomodulatory effect Effects 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000002366 mineral element Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 231100000862 numbness Toxicity 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000036407 pain Effects 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- OQUKIQWCVTZJAF-UHFFFAOYSA-N phenol;sulfuric acid Chemical compound OS(O)(=O)=O.OC1=CC=CC=C1 OQUKIQWCVTZJAF-UHFFFAOYSA-N 0.000 description 1
- 208000026435 phlegm Diseases 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 229940126680 traditional chinese medicines Drugs 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
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- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to a method for extracting gastrodia elata polysaccharide from gastodia elata Bl extracting dregs generated from gastrodin extractum prepared by a pharmaceutical factory. The technical scheme adopted by the method comprises the following steps: placing gastodia elata Bl extracting dregs generated from gastrodin extractum prepared by the pharmaceutical factory into a microwave reactor and performing reflux extraction with water; ultrafiltering a leaching solution with an ultrafiltration membrane which is 150-120 KDa in molecular cut-off quantity; further ultrafiltering a filtered solution with an ultrafiltration membrane which is 20-10 KDa in molecular cutting quantity; freezing and drying a trapped fluid to obtain gastrodia elata polysaccharide. As microwave assistance and ultrafiltration membrane for ultrafiltration are adopted to obtain gastrodia elata polysaccharide, the yield of the gastrodia elata polysaccharide is increased, the purity of the gastrodia elata polysaccharide is improved and the activity of the gastrodia elata polysaccharide is maintained; the method has the characteristics of simple manufacturing technology, short operating time and facilitation of industrialized production, and the produced gastrodia elata polysaccharide can be widely applied to various health food and medicines.
Description
Technical field
The present invention relates to a kind ofly take rhizoma Gastrodiae that pharmaceutical factory prepares Gastrodine medicinal extract to extract residue be the method that raw material extracts gastrodia elata polysaccharide, belong to vegetable active composition and extract manufacture field.
Background technology
Rhizoma Gastrodiae (Gastrodia elata Bl) belongs to the orchid family (Orehidaceae), the perennial heterotrophism herbaceous plant of Gastrodia (Gastrodieae).Have another name called DINGFENGCAO, clutch grass, celestial being's pin, yellow moccasin flower, rhizoma gastrodiae etc., rhizoma Gastrodiae is the conventional rare Chinese medicine of doctor at all times, is also green health treasure, so far the applicating history of existing two thousand years.Rhizoma Gastrodiae is good at diseases such as treatment dizziness and headache, convulsions tic, numbness of the limbs, rheumatism, body pain, language is unsuccessful, blood vessels are obstructed, phlegm carbuncle vapour locks, therefore there be saying of " three towns ", " three is anti-", " mending ", be anti-epileptic, anticonvulsion, rheumatism, calmness, antispastic, analgesia, qi-restoratives.Modern pharmacological research shows, rhizoma Gastrodiae mainly contains four large therapeutic actions: to the calmness of nervous center system, anticonvulsion and analgesic activity; Cardiotonic to cardiovascular systems; There is the effect of resisting oxygen lack and enhancing immunologic function; Meanwhile, rhizoma Gastrodiae also has good health-care effect.The main effective constituent of rhizoma Gastrodiae is the phenoloids such as Gastrodine and gastrodia elata polysaccharide, wherein gastrodia elata polysaccharide have immunomodulatory, hypoglycemic, remove interior free yl, the effect such as delay senility.Separately have multiple mineral element and protein, amino acid, but modern science mainly concentrates on Gastrodine to the research of rhizoma Gastrodiae, and for other composition contained in rhizoma Gastrodiae, particularly gastrodia elata polysaccharide research is less, both system was not deep enough yet not.In existing patent documentation, only there is CN101357192B to take rhizoma Gastrodiae Crude polysaccharides that fresh rhizoma Gastrodiae is raw material and the method for separating and preparing of rhizoma Gastrodiae alcohol soluble substance, fresh rhizoma Gastrodiae completes, broken, add water mill slurry, slurries filter, clear liquid alcohol precipitation, obtain rhizoma Gastrodiae Crude polysaccharides, rhizoma Gastrodiae filter residue uses alcohol diafiltration to extract, and extracting solution is through concentrating, be dried, obtain rhizoma Gastrodiae alcohol soluble substance.Its rhizoma Gastrodiae Crude polysaccharides has comprised protein, protein bound sugar, oligose etc.In non-patent literature: [bright build etc. the separation and purification of rhizoma Gastrodiae water-soluble polysaccharide and physico-chemical property research. Food science, 2008,29 (9): 344-348; Zhao Peng. the research overview of gastrodia elata polysaccharide. Gansu college of traditional Chinese medicine journal, 2008,25 (4): 49-52; Zhu Xiaoxia etc. Extraction Parameters for Polysaccharides of Gastrodia elata Bl optimization. time precious traditional Chinese medicines medicine, 2007,18(4): 906-907; Liu Mingqing etc. Extraction Parameters for Polysaccharides of Gastrodia elata Bl and purifying research. Chinese pharmacists .2011,14 (11): 1593-1596] all take dry rhizoma Gastrodiae as raw material, water extraction, Seaveg method are removed albumen, alcohol precipitation etc. and are prepared gastrodia elata polysaccharide; Have no and take rhizoma Gastrodiae that pharmaceutical factory prepares Gastrodine medicinal extract to extract residue be raw material, membrane sepn is prepared the report of gastrodia elata polysaccharide.
Summary of the invention
The invention provides and a kind ofly take rhizoma Gastrodiae that pharmaceutical factory prepares Gastrodine medicinal extract to extract residue be the method that raw material extracts gastrodia elata polysaccharide, take full advantage of secondary resource, by simple and feasible, can the strong method of action row, the problem such as the step of preparation process that solved gastrodia elata polysaccharide is complicated, production cost is higher.
The technical solution used in the present invention: the rhizoma Gastrodiae that Gastrodine medicinal extract is prepared in pharmaceutical factory extract residue, dry, pulverize after, add 6～15 times of water, in microwave reactor, when microwave power 600～800W, heating is extracted 5～12 minutes, through gallus-type whizzer, filters, and obtains feed liquid 1; Filter residue adds 5～10 times of water yields in microwave power 600～800W, then extracts 2～6 minutes, filters to obtain feed liquid 2; Merge feed liquid 1 and feed liquid 2, the feed liquid of merging is again through the filter of clear tubular-bowl centrifuge essence; It is 0.08～0.20MPa at pressure that essence filtrate liquid adopts molecule cutting quantity 150～120KDa ultra-filtration membrane, when being 20～40 ℃, temperature carries out uf processing, it is 0.08～0.25MPa at pressure that filtered solution continues to adopt the ultra-filtration membrane of molecule cutting quantity 20～10KDa, temperature is 20～40 ℃ and carries out uf processing, collects trapped fluid; Trapped fluid lyophilize, obtains gastrodia elata polysaccharide after pulverizing.
Advantage of the present invention is: the rhizoma Gastrodiae that pharmaceutical factory prepares Gastrodine medicinal extract of take extract residue as raw material, take full advantage of the effective constituent in rhizoma Gastrodiae, reduced the wasting of resources; Gastrodia elata polysaccharide is extracted in microwave-assisted ultra-filtration membrane ultrafiltration simultaneously, and the operating time is short, and the yield of gastrodia elata polysaccharide is greatly improved than traditional water extraction and alcohol precipitation method, and the content of the polysaccharide of acquisition, also higher than the product of water extraction and alcohol precipitation method, has also kept the structure of polysaccharide with active; By phenolsulfuric acid standard measure, measure, the polysaccharide content that present method is extracted is 55～62%.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail, but embodiment is not limitation of the invention.
Embodiment mono-
The rhizoma Gastrodiae that Gastrodine medicinal extract is prepared in pharmaceutical factory extract residue dry, pulverize after, add 15 times of water, in microwave reactor, when microwave power 600W, heating is extracted 10 minutes, filter to obtain feed liquid 1, filter residue adds 8 times of water yields in microwave power 600W, then extracts 4 minutes, filters to obtain feed liquid 2, merge feed liquid 1 and feed liquid 2, the feed liquid of merging is through the filter of clear tubular-bowl centrifuge essence; It is 0.10MPa at pressure that essence filtrate liquid adopts molecule cutting quantity 150KDa ultra-filtration membrane, when being 35 ℃, temperature carries out uf processing, it is 0.09MPa at pressure that filtered solution continues to adopt the ultra-filtration membrane of molecule cutting quantity 20KDa, temperature is 30 ℃ and carries out uf processing, collect trapped fluid, trapped fluid lyophilize, obtains gastrodia elata polysaccharide after pulverizing.
Embodiment bis-
The rhizoma Gastrodiae that Gastrodine medicinal extract is prepared in pharmaceutical factory extract residue dry, pulverize after, add 12 times of water, in microwave reactor, when microwave power 800W, heating is extracted 5 minutes, filter to obtain feed liquid 1, filter residue adds 6 times of water yields in microwave power 800W, then extracts 2 minutes, filters to obtain feed liquid 2, merge feed liquid 1 and feed liquid 2, the feed liquid of merging is through the filter of clear tubular-bowl centrifuge essence; It is 0.15MPa at pressure that essence filtrate liquid adopts molecule cutting quantity 150KDa ultra-filtration membrane, temperature is 30 ℃, carry out uf processing, it is 0.12MPa at pressure that filtered solution continues to adopt the ultra-filtration membrane of molecule cutting quantity 20KDa, temperature is 30 ℃ and carries out uf processing, collect trapped fluid, trapped fluid lyophilize, obtains gastrodia elata polysaccharide after pulverizing.
Embodiment tri-
The rhizoma Gastrodiae that Gastrodine medicinal extract is prepared in pharmaceutical factory extract residue dry, pulverize after, add 10 times of water, in microwave reactor, when microwave power 800W, heating is extracted 6 minutes, filter to obtain feed liquid 1, filter residue adds 8 times of water yields in microwave power 600W, then extracts 4 minutes, filters to obtain feed liquid 2, merge feed liquid 1 and feed liquid 2, the feed liquid of merging is through the filter of clear tubular-bowl centrifuge essence; It is 0.20MPa at pressure that essence filtrate liquid adopts molecule cutting quantity 120KDa ultra-filtration membrane, temperature is 40 ℃ and carries out uf processing, it is 0.15MPa at pressure that filtered solution continues to adopt the ultra-filtration membrane of molecule cutting quantity 10KDa, temperature is 40 ℃ and carries out uf processing, collect trapped fluid, trapped fluid lyophilize, obtains gastrodia elata polysaccharide after pulverizing.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention.All any modifications of doing within the spirit and principles in the present invention, be equal to and replace and improvement etc., within all should being included in protection scope of the present invention.
Claims (3)
1. a gastrodia elata polysaccharide extracting method, described method comprises:
(1) rhizoma Gastrodiae of being prepared by pharmaceutical factory to Gastrodine medicinal extract extract residue, dry, pulverize after, add 6～20 times of water, in microwave reactor, heating is extracted, filter to obtain feed liquid 1, filter residue adds in 5～10 times of water yield microwave reactors and reheats extraction, filter to obtain feed liquid 2, merge feed liquid 1 and feed liquid 2, the feed liquid of merging is again through the filter of clear tubular-bowl centrifuge essence.
(2) smart filtrate liquid employing molecule cutting quantity 150～120KDa ultra-filtration membrane is 0.08～0.20MPa at pressure, temperature is 20～40 ℃ and carries out uf processing, it is 0.08～0.25MPa at pressure that filtered solution continues to adopt the ultra-filtration membrane of molecule cutting quantity 20～10KDa, temperature is 20～40 ℃ and carries out uf processing, collects trapped fluid.
(3) trapped fluid lyophilize, obtains gastrodia elata polysaccharide after pulverizing.
2. the extracting method of a kind of gastrodia elata polysaccharide as claimed in claim 1, is characterized in that: during the extracting through microwave heating of step 1, and microwave power 600～800W, for the first time 5～12 minutes extraction times, for the first time 2～6 minutes extraction times.
3. the extracting method of a kind of gastrodia elata polysaccharide as claimed in claim 1, is characterized in that: the filtration of step 1 is first through gallus-type whizzer coarse filtration, then through the filter of clear tubular-bowl centrifuge essence.
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| CN201310585026.2A CN103665178B (en) | 2013-11-19 | 2013-11-19 | A kind of gastrodia elata polysaccharide extracting method |
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| CN201310585026.2A CN103665178B (en) | 2013-11-19 | 2013-11-19 | A kind of gastrodia elata polysaccharide extracting method |
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| CN103665178A true CN103665178A (en) | 2014-03-26 |
| CN103665178B CN103665178B (en) | 2016-03-16 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105859899A (en) * | 2016-04-10 | 2016-08-17 | 黄河水利职业技术学院 | Rhizoma gastrodiae polysaccharide extraction method |
| CN113754706A (en) * | 2021-09-10 | 2021-12-07 | 广东青云山药业有限公司 | Gastrodia elata extract and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102464725A (en) * | 2010-11-17 | 2012-05-23 | 中国科学院上海药物研究所 | Gastrodia elata BL. polysaccharide and degradation products thereof and preparation methods and applications of degradation products |
| CN103242461A (en) * | 2012-02-06 | 2013-08-14 | 陕西理工学院 | Novel method for simultaneously extracting gastrodia elata polysaccharide and gastrodine |
-
2013
- 2013-11-19 CN CN201310585026.2A patent/CN103665178B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102464725A (en) * | 2010-11-17 | 2012-05-23 | 中国科学院上海药物研究所 | Gastrodia elata BL. polysaccharide and degradation products thereof and preparation methods and applications of degradation products |
| CN103242461A (en) * | 2012-02-06 | 2013-08-14 | 陕西理工学院 | Novel method for simultaneously extracting gastrodia elata polysaccharide and gastrodine |
Non-Patent Citations (3)
| Title |
|---|
| 李志英等: ""微波法提取天麻多糖的研究"", 《山西大学学报（自然科学版）》, vol. 31, no. 4, 15 November 2008 (2008-11-15) * |
| 杨义芳等: "《中药提取分离新技术》", 30 April 2010, article ""膜技术在中药提取分离、制备中的应用实例"" * |
| 金虹等: ""天麻提取物工艺及其功效成分含量的分析"", 《中草药》, vol. 32, no. 5, 25 May 2009 (2009-05-25) * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105859899A (en) * | 2016-04-10 | 2016-08-17 | 黄河水利职业技术学院 | Rhizoma gastrodiae polysaccharide extraction method |
| CN113754706A (en) * | 2021-09-10 | 2021-12-07 | 广东青云山药业有限公司 | Gastrodia elata extract and preparation method thereof |
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| CN103665178B (en) | 2016-03-16 |
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Effective date of registration: 20231126 Address after: No. 101, Building 10, Wumenggu Town, Longquan Street, Zhaoyang District, Zhaotong City, Yunnan Province, 657099 Patentee after: Yunnan Bangming Tianma Biotechnology Co.,Ltd. Address before: Yunnan city of Kunming Province, Kunming University of Science and Technology lotus Huayuan 37-3-206 Patentee before: Liu Xiangyi |