CN103288976A - High-efficiency method for extracting astragalus polysaccharide - Google Patents
High-efficiency method for extracting astragalus polysaccharide Download PDFInfo
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- CN103288976A CN103288976A CN 201310179270 CN201310179270A CN103288976A CN 103288976 A CN103288976 A CN 103288976A CN 201310179270 CN201310179270 CN 201310179270 CN 201310179270 A CN201310179270 A CN 201310179270A CN 103288976 A CN103288976 A CN 103288976A
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Abstract
The invention discloses a high-efficiency method for extracting astragalus polysaccharide. The method comprises the following steps of: adding ethanol with the volume concentration of 95 percent into astragalus membranaceus for reflux degreasing, and filtering the mixture to obtain a filtered liquid; concentrating the filtered liquid until the volume of the filtered liquid is 1/5 to obtain a concentrated liquid; adding protease into the concentrated liquid for enzymolysis, boiling the concentrated liquid, and centrifugating the concentrated liquid to obtain supernate; adding cellulose into the supernate, boiling the supernate for 10-20 minutes, filtering the supernate, adding ethanol with the mass concentration of 80 percent into the filtered liquid, performing precipitation, standing the filtered liquid overnight, centrifugating the filtered liquid, and collecting precipitates; performing ultrasonic hydrolysis on the precipitates, diluting the precipitates by deionized water, and concentrating the deionized water to obtain white solids; and adding water into the white solids, purifying and separating the white solids by gel column chromatography, diluting the white solids by the deionized water, concentrating the diluted liquid, and drying the diluted liquid to obtain the astragalus polysaccharide. According to the method, degreasing is executed at first, and then the filtered liquid is concentrated; the protease is added into the concentrated liquid; after enzymolysis is executed, the cellulose is added into the concentrated liquid; the degreasing is executed again, and water dilution is executed; the obtained polysaccharide is more pure; the extraction rate is higher; and the cost is low.
Description
Technical field
The present invention relates to a kind of extracting method of vegetable polysaccharides, relate in particular to a kind of efficient extraction of Astragalus Polysaccharides in Astragalus method.
Background technology
Astragalus polysaccharides is one of the most effective composition in the Radix Astragali.The method of disclosed a kind of high purity astragalus polysaccharides, centrifugal after the constant temperature microwave extraction, the supernatant liquor decolorizing with activated carbon, alcohol precipitation, gel permeation chromatography gets final product, but this method length consuming time, materials are many, in the leaching process, note temperature variation constantly.Therefore, need provide a kind of new technical scheme to solve the problems referred to above.
Summary of the invention
The purpose of this invention is to provide the simple efficient extraction of Astragalus Polysaccharides in Astragalus method of a kind of technology.
The technical solution used in the present invention:
A kind of efficient extraction of Astragalus Polysaccharides in Astragalus method may further comprise the steps:
A, get the Radix Astragali, add volumetric concentration and be the degreasing that refluxes of 95% ethanol, filter, get filtrate;
B, filtrate is concentrated into 1/5 of filtrate volume, obtains concentrated solution, add proteolytic enzyme in concentrated solution, the mol ratio of proteolytic enzyme and the Radix Astragali is 1-3:1, under 35-40 ℃ of condition, and enzymolysis 3-5 hour, boil 10-20 minute, centrifugal, get supernatant liquor;
Add Mierocrystalline cellulose in c, the supernatant liquor, boiled 10-20 minute, filter, in filtrate, add the ethanol of mass concentration 80%, precipitation, standing over night, centrifugal after, the collecting precipitation thing;
D, throw out carry out ultrasonic water dissolution, and the deionized water wash-out concentrates, and obtains white solid;
E, white solid are dissolved in water, and through the gel filtration chromatography purifies and separates, the deionized water wash-out concentrates, and drying gets astragalus polysaccharides.
In the d step, add the deionized water wash-out, the quality of deionized water is sedimentary 5-8 times.
In the e step, drying is-4 ℃ of lyophilizes under-1 ℃ of condition.
Advantage of the present invention is: first degreasing, again filtrate is concentrated, add proteolytic enzyme in the concentrated solution, add Mierocrystalline cellulose behind the enzymolysis after, degreasing again, water elution, the polysaccharide that obtains is purer, extraction yield is higher, and is with low cost.
Embodiment
Following embodiment only is used for explanation the present invention, but can not limit protection scope of the present invention.
The technical solution used in the present invention:
Embodiment 1
A kind of efficient extraction of Astragalus Polysaccharides in Astragalus method may further comprise the steps:
A, get the Radix Astragali, add volumetric concentration and be the degreasing that refluxes of 95% ethanol, filter, get filtrate;
B, filtrate is concentrated into 1/5 of filtrate volume, obtains concentrated solution, add proteolytic enzyme in concentrated solution, the mol ratio of proteolytic enzyme and the Radix Astragali is 1:1, and under 35 ℃ of conditions, enzymolysis 3 hours boils 10 minutes, and is centrifugal, gets supernatant liquor;
Add Mierocrystalline cellulose in c, the supernatant liquor, boiled 10 minutes, filter, in filtrate, add the ethanol of mass concentration 80%, precipitation, standing over night, centrifugal after, the collecting precipitation thing;
D, throw out carry out ultrasonic water dissolution, the deionized water wash-out, and the quality of deionized water is sedimentary 5 times.Concentrate, obtain white solid;
E, white solid are dissolved in water, and through the gel filtration chromatography purifies and separates, the deionized water wash-out concentrates, drying, and drying is-4 ℃ of lyophilizes under the condition, gets astragalus polysaccharides.
Embodiment 2
A kind of efficient extraction of Astragalus Polysaccharides in Astragalus method may further comprise the steps:
A, get the Radix Astragali, add volumetric concentration and be the degreasing that refluxes of 95% ethanol, filter, get filtrate;
B, filtrate is concentrated into 1/5 of filtrate volume, obtains concentrated solution, add proteolytic enzyme in concentrated solution, the mol ratio of proteolytic enzyme and the Radix Astragali is 2:1, and under 37 ℃ of conditions, enzymolysis 4 hours boils 15 minutes, and is centrifugal, gets supernatant liquor;
Add Mierocrystalline cellulose in c, the supernatant liquor, boiled 15 minutes, filter, in filtrate, add the ethanol of mass concentration 80%, precipitation, standing over night, centrifugal after, the collecting precipitation thing;
D, throw out carry out ultrasonic water dissolution, the deionized water wash-out, and the quality of deionized water is sedimentary 6 times.Concentrate, obtain white solid;
E, white solid are dissolved in water, and through the gel filtration chromatography purifies and separates, the deionized water wash-out concentrates, drying, and drying is-2 ℃ of lyophilizes under the condition, gets astragalus polysaccharides.
Embodiment 3
A kind of efficient extraction of Astragalus Polysaccharides in Astragalus method may further comprise the steps:
A, get the Radix Astragali, add volumetric concentration and be the degreasing that refluxes of 95% ethanol, filter, get filtrate;
B, filtrate is concentrated into 1/5 of filtrate volume, obtains concentrated solution, add proteolytic enzyme in concentrated solution, the mol ratio of proteolytic enzyme and the Radix Astragali is 3:1, and under 40 ℃ of conditions, enzymolysis 5 hours boils 20 minutes, and is centrifugal, gets supernatant liquor;
Add Mierocrystalline cellulose in c, the supernatant liquor, boiled 20 minutes, filter, in filtrate, add the ethanol of mass concentration 80%, precipitation, standing over night, centrifugal after, the collecting precipitation thing;
D, throw out carry out ultrasonic water dissolution, the deionized water wash-out, and the quality of deionized water is sedimentary 8 times.Concentrate, obtain white solid;
E, white solid are dissolved in water, and through the gel filtration chromatography purifies and separates, the deionized water wash-out concentrates, and drying, drying are 1 ℃ of lyophilize under the condition, get astragalus polysaccharides.
Elder generation's degreasing concentrates filtrate again, adds proteolytic enzyme in the concentrated solution, add Mierocrystalline cellulose behind the enzymolysis after, degreasing again, water elution, the polysaccharide that obtains is purer, extraction yield is higher, and is with low cost.
Claims (3)
1. an efficient extraction of Astragalus Polysaccharides in Astragalus method is characterized in that, may further comprise the steps:
A, get the Radix Astragali, add volumetric concentration and be the degreasing that refluxes of 95% ethanol, filter, get filtrate;
B, filtrate is concentrated into 1/5 of filtrate volume, obtains concentrated solution, add proteolytic enzyme in concentrated solution, the mol ratio of proteolytic enzyme and the Radix Astragali is 1-3:1, under 35-40 ℃ of condition, and enzymolysis 3-5 hour, boil 10-20 minute, centrifugal, get supernatant liquor;
Add Mierocrystalline cellulose in c, the supernatant liquor, boiled 10-20 minute, filter, in filtrate, add the ethanol of mass concentration 80%, precipitation, standing over night, centrifugal after, the collecting precipitation thing;
D, throw out carry out ultrasonic water dissolution, and the deionized water wash-out concentrates, and obtains white solid;
E, white solid are dissolved in water, and through the gel filtration chromatography purifies and separates, the deionized water wash-out concentrates, and drying gets astragalus polysaccharides.
2. a kind of efficient extraction of Astragalus Polysaccharides in Astragalus method according to claim 1 is characterized in that: in the d step, add the deionized water wash-out, the quality of deionized water be sedimentary 5-8 doubly.
3. a kind of efficient extraction of Astragalus Polysaccharides in Astragalus method according to claim 1, it is characterized in that: in the e step, drying is-4 ℃ of lyophilizes under-1 ℃ of condition.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201310179270 CN103288976A (en) | 2013-05-15 | 2013-05-15 | High-efficiency method for extracting astragalus polysaccharide |
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| CN 201310179270 CN103288976A (en) | 2013-05-15 | 2013-05-15 | High-efficiency method for extracting astragalus polysaccharide |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104311689A (en) * | 2014-11-11 | 2015-01-28 | 济南凯因生物科技有限公司 | Extraction method of radix astragali polysaccharide |
| CN105504079A (en) * | 2015-12-14 | 2016-04-20 | 陈燕妮 | Process for producing astragalus polysaccharide by using ultrasonic technology |
-
2013
- 2013-05-15 CN CN 201310179270 patent/CN103288976A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104311689A (en) * | 2014-11-11 | 2015-01-28 | 济南凯因生物科技有限公司 | Extraction method of radix astragali polysaccharide |
| CN105504079A (en) * | 2015-12-14 | 2016-04-20 | 陈燕妮 | Process for producing astragalus polysaccharide by using ultrasonic technology |
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Application publication date: 20130911 |