CN102775509A - Fractional extraction method of crude polysaccharide with Chinese herbal medicine - Google Patents
Fractional extraction method of crude polysaccharide with Chinese herbal medicine Download PDFInfo
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Abstract
The invention relates to an extraction process of crude polysaccharide containing plant functional components, and particularly relates to a fractional extraction method of crude polysaccharide with Chinese herbal medicine, which is characterized by comprising the following steps of: extracting Chinese herbal medicine containing crude polysaccharide with hot water, adding pectinase to the obtained solution for treatment, clarification, concentration and filtration, precipitating, separating and extracting crude polysaccharide with alcohol precipitation from low to high respectively according to eight concentration gradient by different alcohol concentration. The fractional extraction method provided by the invention comes from the practical application and summary in the studying process of crude polysaccharide. Alcohol is generally used as solvent or extraction additive in processing and practical application. So classifying crude polysaccharide according to the different solubilit in different concentrations of alcohol has strong practicality and feasibility. The operation method of extraction is simple and the cost is low.
Description
Technical field
The present invention relates to the extraction process of vegetalitas effective component Crude polysaccharides, especially a kind of method with Chinese medicinal materials grading extraction Crude polysaccharides.
Background technology
Along with the development of modern age biotechnology and research means, the effective component of many Chinese medicinal materialss has obtained affirmation.Like matrimony vine, the matrimony vine Crude polysaccharides is the effective component of its strengthening immunity and alleviating physical fatigue.Many about the extraction of Crude polysaccharides research, taking after the hot water extraction method with 80% alcohol precipitation extraction Crude polysaccharides earlier is that maturation also is to be used the most frequent method.The method of Crude polysaccharides being carried out grading extraction is also a lot, has the DEAE of employing cellulose column staging to obtain LBP-I, LBP-II, LBP-III, LBP-V, LBP-V.Also have and adopt ultrafiltration process to carry out the grading extraction LBP by LBP molecular weight size, obtain molecular weight below 5000,5000-1 ten thousand, 10,000-30,000,30,000-100,000,100,000-300,000 and 300,000 above 6 other methods of level.But the end is seen with Crude polysaccharides different character of solvability in the spirituous solution of different concns up to now, extracts the method for Crude polysaccharides.
Crude polysaccharides is the functional polysaccharide of one type of complicacy, is each saccharoidal mixtinite with the Crude polysaccharides of 80% (V/V) alcoholic extraction.DEAE cellulose column staging only is the embodiment of different Crude polysaccharides different in kind on the DEAE cellulose column, has limitation.Ultrafiltration process grading extraction Crude polysaccharides is a kind of physical method, and grading effect is poor, and each high-molecular weight component all possibly contain the Crude polysaccharides of LMWC.
Summary of the invention
The purpose of this invention is to provide a kind of method, so that further study the chemical property and the biological activity of these different stage Crude polysaccharides with Chinese medicinal materials grading extraction Crude polysaccharides.
A kind of method with Chinese medicinal materials grading extraction Crude polysaccharides; Its special feature is; The Chinese medicinal materials that comprises the steps: to contain Crude polysaccharides with hot water extraction after; The solution that obtains adds polygalacturonase again to be handled, clarify, concentrates and filter, and divides 8 concentration gradients by different ethanol concns, extracts Crude polysaccharides with the alcohol deposition method precipitate and separate respectively from low to high.
Further; Comprise the steps: to contain the water rehydration of the Chinese medicinal materials of Crude polysaccharides with 5-10 times of weight, be heated to 60-100 ℃, hold-time 20-60min extracts Crude polysaccharides; Separate the dregs of a decoction and soup then; Taking liquid, is clarified behind the 24h diatomite filtration again and is handled with polygalacturonase 40-55 ℃ of insulation 3h through 10-30PPM food, is concentrated to the 1/10-9/10 of original volume at last; With the soup after concentrating; Using edible ethanol to adjust soup alcoholic strength V/V successively is 10%, 20%, 30%, 40%, 50%, 60%, 70% and 80%; Then soup was kept 20 days under this ethanol concn respectively; Thereby Crude polysaccharides is fully precipitated, promptly obtain the Crude polysaccharides of different stage again through the spinning post precipitation.
Further, comprise the steps:
(1) it is for use to take by weighing the Chinese medicinal materials that contains Crude polysaccharides;
The Chinese medicinal materials that (2) will contain Crude polysaccharides adds the purified water of 5-10 times of raw material weight; Be heated to 60-100 ℃ of refluxing extraction 20-60min; The filtering separation extracting solution obtains extracting for the first time soup then; Obtain extracting soup for the second time after the purified water that adds 2-5 times of raw material weight in the dregs of a decoction once more repeats to extract, merge 2 times and extract soup;
The extraction soup that (3) will obtain adds 10-30PPM food and mixes at 40-55 ℃ of insulation 3h with polygalacturonase, uses diatomite filtration behind the clarification 24h, removes precipitated impurities, gets the soup clear liquid;
(4) 1/10-9/10 that the soup clear liquid that obtains is concentrated into original volume in vacuum concentration equipment doubly;
(5) concentrate back soup alcoholic strength to 10%V/V with edible ethanol adjustment, behind the mixing after leaving standstill 20 days under 10 ℃ of conditions spinning clear liquid and deposition, the collecting precipitation material is dried to moisture content and is lower than 13% and is Crude polysaccharides A;
Concentrate back soup alcoholic strength to 20%V/V with edible ethanol adjustment, behind the mixing after leaving standstill 20 days under 10 ℃ of conditions spinning clear liquid and deposition, the collecting precipitation material is dried to moisture content and is lower than 13% and is Crude polysaccharides B;
Concentrate back soup alcoholic strength to 30%V/V with edible ethanol adjustment, behind the mixing after leaving standstill 20 days under 10 ℃ of conditions spinning clear liquid and deposition, the collecting precipitation material is dried to moisture content and is lower than 13% and is Crude polysaccharides C;
Concentrate back soup alcoholic strength to 40%V/V with edible ethanol adjustment, behind the mixing after leaving standstill 20 days under 10 ℃ of conditions spinning clear liquid and deposition, the collecting precipitation material is dried to moisture content and is lower than 13% and is Crude polysaccharides D;
Concentrate back soup alcoholic strength to 50%V/V with edible ethanol adjustment, behind the mixing after leaving standstill 20 days under 10 ℃ of conditions spinning clear liquid and deposition, the collecting precipitation material is dried to moisture content and is lower than 13% and is Crude polysaccharides E;
Concentrate back soup alcoholic strength to 60%V/V with edible ethanol adjustment, behind the mixing after leaving standstill 20 days under 10 ℃ of conditions spinning clear liquid and deposition, the collecting precipitation material is dried to moisture content and is lower than 13% and is Crude polysaccharides F;
Concentrate back soup alcoholic strength to 70%V/V with edible ethanol adjustment, behind the mixing after leaving standstill 20 days under 10 ℃ of conditions spinning clear liquid and deposition, the collecting precipitation material is dried to moisture content and is lower than 13% and is Crude polysaccharides G;
Concentrate back soup alcoholic strength to 80%V/V with edible ethanol adjustment, behind the mixing after leaving standstill 20 days under 10 ℃ of conditions spinning clear liquid and deposition, the collecting precipitation material is dried to moisture content and is lower than 13% and is Crude polysaccharides H.
The Chinese medicinal materials that wherein contains Crude polysaccharides is wolfberry fruit, Fructus Schisandrae Chinensis, the Radix Astragali or glossy ganoderma.
Wherein edible ethanol concentration is 95%V/V.
Wherein the drying of sedimentable matter is carried out in vacuum drying oven, drying temperature 50-80 ℃.
Fractional extraction method of the present invention is summed up from the practical application and the conclusion in the research process of Crude polysaccharides; Generally all be as solvent or extraction auxiliary agent in processing and practical application with alcohol; Therefore by the solvability difference of Crude polysaccharides in different concns alcohol Crude polysaccharides is carried out classification; Have very strong practicality and feasibility, and it is simple, with low cost to extract working method.
Embodiment
Experiment material and equipment that the embodiment of the invention adopted are following:
| Device name | Unit type | Manufacturer |
| Matrimony vine | 500 | Ningxia HongZhongning medlar Products Co., Ltd |
| Polygalacturonase | 3.5 ten thousand u/g | Ningxia He Shi wall biotech company |
| Efficient circulation is extracted unit | TQWZ-3-500 | Wenzhou, Zhejiang Province auspicious grand medicine ltd |
| Vacuum concentration pot | NZ-500 | Wenzhou, Zhejiang Province auspicious grand medicine ltd |
| Whizzer | 80-2B | Anting Scientific Instrument Factory, Shanghai |
| Vacuum drying oven | ZK-35AB | Shen, Shanghai light instrument ltd |
Embodiment 1:
Take by weighing after wolfberry fruit 1000g cleans with flowing water, add the purified water of 5000g (5000ml).
Be heated to 60 ℃ at the efficient circulation extracting machine, refluxing extraction 20min after-filtration obtains extracting for the first time soup.The purified water that adds 5000g (5000ml) in the dregs of a decoction once more repeats to obtain extracting solution for the second time after the said extracted step, merges No. 2 extracting solutions and gets the 6000ml soup.
Fluid temperature adds 10PPM food and uses the polygalacturonase mixing in the time of 55 ℃, insulation 3h enzyme is handled, and use diatomite filtration after leaving standstill 24h, removes precipitated impurities, must the soup clear liquid.
The soup clear liquid is concentrated into 9/10 times of original volume in vacuum concentration equipment.
Adjust the soup alcoholic strength to 10% mixing with 95% (V/V) edible ethanol; After leaving standstill 20 days under 10 ℃ of conditions; Whizzer is with 3000 rev/mins of spinning clear liquids and deposition, and the collecting precipitation material is lower than 13% through vacuum drying oven dry (60 ℃ of drying temperatures, down together) to moisture content and is Crude polysaccharides A; Adjust above-mentioned steps gained soup alcoholic strength to 20% mixing with 95% (V/V) edible ethanol; After leaving standstill 20 days under 10 ℃ of conditions; Whizzer is with 3000 rev/mins of spinning clear liquids and deposition, and the collecting precipitation material is dried to moisture content through vacuum drying oven and is lower than 13% and is Crude polysaccharides B; Adjust above-mentioned steps gained soup alcoholic strength to 30% mixing with 95% (V/V) edible ethanol; After leaving standstill 20 days under 10 ℃ of conditions; Whizzer is with 3000 rev/mins of spinning clear liquids and deposition, and the collecting precipitation material is dried to moisture content through vacuum drying oven and is lower than 13% and is Crude polysaccharides C; Adjust above-mentioned steps gained soup alcoholic strength to 40% mixing with 95% (V/V) edible ethanol; After leaving standstill 20 days under 10 ℃ of conditions; Whizzer is with 3000 rev/mins of spinning clear liquids and deposition, and the collecting precipitation material is dried to moisture content through vacuum drying oven and is lower than 13% and is Crude polysaccharides D; Adjust above-mentioned steps gained soup alcoholic strength to 50% mixing with 95% (V/V) edible ethanol; After leaving standstill 20 days under 10 ℃ of conditions; Whizzer is with 3000 rev/mins of spinning clear liquids and deposition, and the collecting precipitation material is dried to moisture content through vacuum drying oven and is lower than 13% and is Crude polysaccharides E; Adjust above-mentioned steps gained soup alcoholic strength to 60% mixing with 95% (V/V) edible ethanol; After leaving standstill 20 days under 10 ℃ of conditions; Whizzer is with 3000 rev/mins of spinning clear liquids and deposition, and the collecting precipitation material is dried to moisture content through vacuum drying oven and is lower than 13% and is Crude polysaccharides F; Adjust above-mentioned steps gained soup alcoholic strength to 70% mixing with 95% (V/V) edible ethanol; After leaving standstill 20 days under 10 ℃ of conditions; Whizzer is with 3000 rev/mins of spinning clear liquids and deposition, and the collecting precipitation material is dried to moisture content through vacuum drying oven and is lower than 13% and is Crude polysaccharides G; Adjust above-mentioned steps gained soup alcoholic strength to 80% mixing with 95% (V/V) edible ethanol; After leaving standstill 20 days under 10 ℃ of conditions; Whizzer is with 3000 rev/mins of spinning clear liquids and deposition, and the collecting precipitation material is dried to moisture content through vacuum drying oven and is lower than 13% and is Crude polysaccharides H.
Embodiment 2:
Take by weighing after wolfberry fruit 1000g cleans with flowing water, add the purified water of 7000g (7000ml).
Be heated to 70 ℃ at the efficient circulation extracting machine, refluxing extraction 30min after-filtration obtains extracting for the first time soup.The purified water that adds 2000g (2000ml) in the dregs of a decoction once more repeats to obtain extracting solution for the second time after the said extracted step, merges No. 2 extracting solutions and gets the 6000ml soup.
Fluid temperature adds 10PPM food and mixes with polygalacturonase in the time of 50 ℃, insulation 3h enzyme is handled, and use diatomite filtration after leaving standstill 24h, removes precipitated impurities, must the soup clear liquid.
The soup clear liquid is concentrated into 4/5 times of original volume in vacuum concentration equipment.
Adjust the soup alcoholic strength to 10% mixing with 95% (V/V) edible ethanol; After leaving standstill 20 days under 10 ℃ of conditions; Whizzer is with 3000 rev/mins of spinning clear liquids and deposition, and the collecting precipitation material is lower than 13% through vacuum drying oven dry (50 ℃ of drying temperatures, down together) to moisture content and is Crude polysaccharides A; Adjust above-mentioned steps gained soup alcoholic strength to 20% mixing with 95% (V/V) edible ethanol; After leaving standstill 20 days under 10 ℃ of conditions; Whizzer is with 3000 rev/mins of spinning clear liquids and deposition, and the collecting precipitation material is dried to moisture content through vacuum drying oven and is lower than 13% and is Crude polysaccharides B; Adjust above-mentioned steps gained soup alcoholic strength to 30% mixing with 95% (V/V) edible ethanol; After leaving standstill 20 days under 10 ℃ of conditions; Whizzer is with 3000 rev/mins of spinning clear liquids and deposition, and the collecting precipitation material is dried to moisture content through vacuum drying oven and is lower than 13% and is Crude polysaccharides C; Adjust above-mentioned steps gained soup alcoholic strength to 40% mixing with 95% (V/V) edible ethanol; After leaving standstill 20 days under 10 ℃ of conditions; Whizzer is with 3000 rev/mins of spinning clear liquids and deposition, and the collecting precipitation material is dried to moisture content through vacuum drying oven and is lower than 13% and is Crude polysaccharides D; Adjust above-mentioned steps gained soup alcoholic strength to 50% mixing with 95% (V/V) edible ethanol; After leaving standstill 20 days under 10 ℃ of conditions; Whizzer is with 3000 rev/mins of spinning clear liquids and deposition, and the collecting precipitation material is dried to moisture content through vacuum drying oven and is lower than 13% and is Crude polysaccharides E; Adjust above-mentioned steps gained soup alcoholic strength to 60% mixing with 95% (V/V) edible ethanol; After leaving standstill 20 days under 10 ℃ of conditions; Whizzer is with 3000 rev/mins of spinning clear liquids and deposition, and the collecting precipitation material is dried to moisture content through vacuum drying oven and is lower than 13% and is Crude polysaccharides F; Adjust above-mentioned steps gained soup alcoholic strength to 70% mixing with 95% (V/V) edible ethanol; After leaving standstill 20 days under 10 ℃ of conditions; Whizzer is with 3000 rev/mins of spinning clear liquids and deposition, and the collecting precipitation material is dried to moisture content through vacuum drying oven and is lower than 13% and is Crude polysaccharides G; Adjust above-mentioned steps gained soup alcoholic strength to 80% mixing with 95% (V/V) edible ethanol; After leaving standstill 20 days under 10 ℃ of conditions; Whizzer is with 3000 rev/mins of spinning clear liquids and deposition, and the collecting precipitation material is dried to moisture content through vacuum drying oven and is lower than 13% and is Crude polysaccharides H.
Embodiment 3:
Take by weighing after wolfberry fruit 1000g cleans with flowing water, add the purified water of 8000g (8000ml).
Be heated to 80 ℃ at the efficient circulation extracting machine, refluxing extraction 20min after-filtration obtains extracting for the first time soup.The purified water that adds 3000g (3000ml) in the dregs of a decoction once more repeats to obtain extracting solution for the second time after the said extracted step, merges No. 2 extracting solutions and gets the 7000ml soup.
Fluid temperature adds 20PPM food and uses the polygalacturonase mixing in the time of 45 ℃, insulation 3h enzyme is handled, and use diatomite filtration after leaving standstill 24h, removes precipitated impurities, must the soup clear liquid.
The soup clear liquid is concentrated into 1/2 times of original volume in vacuum concentration equipment.
Adjust the soup alcoholic strength to 10% mixing with 95% (V/V) edible ethanol; After leaving standstill 20 days under 10 ℃ of conditions; Whizzer is with 3000 rev/mins of spinning clear liquids and deposition, and the collecting precipitation material is lower than 13% through vacuum drying oven dry (65 ℃ of drying temperatures, down together) to moisture content and is Crude polysaccharides A; Adjust above-mentioned steps gained soup alcoholic strength to 20% mixing with 95% (V/V) edible ethanol; After leaving standstill 20 days under 10 ℃ of conditions; Whizzer is with 3000 rev/mins of spinning clear liquids and deposition, and the collecting precipitation material is dried to moisture content through vacuum drying oven and is lower than 13% and is Crude polysaccharides B; Adjust above-mentioned steps gained soup alcoholic strength to 30% mixing with 95% (V/V) edible ethanol; After leaving standstill 20 days under 10 ℃ of conditions; Whizzer is with 3000 rev/mins of spinning clear liquids and deposition, and the collecting precipitation material is dried to moisture content through vacuum drying oven and is lower than 13% and is Crude polysaccharides C; Adjust above-mentioned steps gained soup alcoholic strength to 40% mixing with 95% (V/V) edible ethanol; After leaving standstill 20 days under 10 ℃ of conditions; Whizzer is with 3000 rev/mins of spinning clear liquids and deposition, and the collecting precipitation material is dried to moisture content through vacuum drying oven and is lower than 13% and is Crude polysaccharides D; Adjust above-mentioned steps gained soup alcoholic strength to 50% mixing with 95% (V/V) edible ethanol; After leaving standstill 20 days under 10 ℃ of conditions; Whizzer is with 3000 rev/mins of spinning clear liquids and deposition, and the collecting precipitation material is dried to moisture content through vacuum drying oven and is lower than 13% and is Crude polysaccharides E; Adjust above-mentioned steps gained soup alcoholic strength to 60% mixing with 95% (V/V) edible ethanol; After leaving standstill 20 days under 10 ℃ of conditions; Whizzer is with 3000 rev/mins of spinning clear liquids and deposition, and the collecting precipitation material is dried to moisture content through vacuum drying oven and is lower than 13% and is Crude polysaccharides F; Adjust above-mentioned steps gained soup alcoholic strength to 70% mixing with 95% (V/V) edible ethanol; After leaving standstill 20 days under 10 ℃ of conditions; Whizzer is with 3000 rev/mins of spinning clear liquids and deposition, and the collecting precipitation material is dried to moisture content through vacuum drying oven and is lower than 13% and is Crude polysaccharides G; Adjust above-mentioned steps gained soup alcoholic strength to 80% mixing with 95% (V/V) edible ethanol; After leaving standstill 20 days under 10 ℃ of conditions; Whizzer is with 3000 rev/mins of spinning clear liquids and deposition, and the collecting precipitation material is dried to moisture content through vacuum drying oven and is lower than 13% and is Crude polysaccharides H.
Embodiment 4:
Take by weighing after wolfberry fruit 1000g cleans with flowing water, add the purified water of 10000g (10000ml).
Be heated to 100 ℃ at the efficient circulation extracting machine, refluxing extraction 60min after-filtration obtains extracting for the first time soup.The purified water that adds 2000g (2000ml) in the dregs of a decoction once more repeats to obtain extracting solution for the second time after the said extracted step, merges No. 2 extracting solutions and gets the 8000ml soup.
Fluid temperature adds 30PPM food and uses the polygalacturonase mixing in the time of 40 ℃, insulation 3h enzyme is handled, and use diatomite filtration after leaving standstill 24h, removes precipitated impurities, must the soup clear liquid.
The soup clear liquid is concentrated into 1/10 times of original volume in vacuum concentration equipment.
Adjust the soup alcoholic strength to 10% mixing with 95% (V/V) edible ethanol; After leaving standstill 20 days under 10 ℃ of conditions; Whizzer is with 3000 rev/mins of spinning clear liquids and deposition, and the collecting precipitation material is lower than 13% through vacuum drying oven dry (60 ℃ of drying temperatures, down together) to moisture content and is Crude polysaccharides A; Adjust above-mentioned steps gained soup alcoholic strength to 20% mixing with 95% (V/V) edible ethanol; After leaving standstill 20 days under 10 ℃ of conditions; Whizzer is with 3000 rev/mins of spinning clear liquids and deposition, and the collecting precipitation material is dried to moisture content through vacuum drying oven and is lower than 13% and is Crude polysaccharides B; Adjust above-mentioned steps gained soup alcoholic strength to 30% mixing with 95% (V/V) edible ethanol; After leaving standstill 20 days under 10 ℃ of conditions; Whizzer is with 3000 rev/mins of spinning clear liquids and deposition, and the collecting precipitation material is dried to moisture content through vacuum drying oven and is lower than 13% and is Crude polysaccharides C; Adjust above-mentioned steps gained soup alcoholic strength to 40% mixing with 95% (V/V) edible ethanol; After leaving standstill 20 days under 10 ℃ of conditions; Whizzer is with 3000 rev/mins of spinning clear liquids and deposition, and the collecting precipitation material is dried to moisture content through vacuum drying oven and is lower than 13% and is Crude polysaccharides D; Adjust above-mentioned steps gained soup alcoholic strength to 50% mixing with 95% (V/V) edible ethanol; After leaving standstill 20 days under 10 ℃ of conditions; Whizzer is with 3000 rev/mins of spinning clear liquids and deposition, and the collecting precipitation material is dried to moisture content through vacuum drying oven and is lower than 13% and is Crude polysaccharides E; Adjust above-mentioned steps gained soup alcoholic strength to 60% mixing with 95% (V/V) edible ethanol; After leaving standstill 20 days under 10 ℃ of conditions; Whizzer is with 3000 rev/mins of spinning clear liquids and deposition, and the collecting precipitation material is dried to moisture content through vacuum drying oven and is lower than 13% and is Crude polysaccharides F; Adjust above-mentioned steps gained soup alcoholic strength to 70% mixing with 95% (V/V) edible ethanol; After leaving standstill 20 days under 10 ℃ of conditions; Whizzer is with 3000 rev/mins of spinning clear liquids and deposition, and the collecting precipitation material is dried to moisture content through vacuum drying oven and is lower than 13% and is Crude polysaccharides G; Adjust above-mentioned steps gained soup alcoholic strength to 80% mixing with 95% (V/V) edible ethanol; After leaving standstill 20 days under 10 ℃ of conditions; Whizzer is with 3000 rev/mins of spinning clear liquids and deposition, and the collecting precipitation material is dried to moisture content through vacuum drying oven and is lower than 13% and is Crude polysaccharides H.
Claims (6)
1. method with Chinese medicinal materials grading extraction Crude polysaccharides; It is characterized in that; The Chinese medicinal materials that comprises the steps: to contain Crude polysaccharides with hot water extraction after; The solution that obtains adds polygalacturonase again to be handled, clarify, concentrates and filter, and divides 8 concentration gradients by different ethanol concns, extracts Crude polysaccharides with the alcohol deposition method precipitate and separate respectively from low to high.
2. a kind of method with Chinese medicinal materials grading extraction Crude polysaccharides as claimed in claim 1 is characterized in that, comprises the steps: the water rehydration of the Chinese medicinal materials that contains Crude polysaccharides with 5-10 times of weight; Be heated to 60-100 ℃; Hold-time 20-60min extracts Crude polysaccharides, separates the dregs of a decoction and soup then, and taking liquid is incubated 3h through 10-30PPM food with polygalacturonase 40-55 ℃; Diatomite filtration is handled after clarifying 24h again, is concentrated to the 1/10-9/10 of original volume at last; With the soup after concentrating; Using edible ethanol to adjust soup alcoholic strength V/V successively is 10%, 20%, 30%, 40%, 50%, 60%, 70% and 80%; Then soup was kept 20 days under this ethanol concn respectively; Thereby Crude polysaccharides is fully precipitated, promptly obtain the Crude polysaccharides of different stage again through the spinning post precipitation.
3. according to claim 1 or claim 2 a kind of method with Chinese medicinal materials grading extraction Crude polysaccharides is characterized in that, comprises the steps:
(1) it is for use to take by weighing the Chinese medicinal materials that contains Crude polysaccharides;
The Chinese medicinal materials that (2) will contain Crude polysaccharides adds the purified water of 5-10 times of raw material weight; Be heated to 60-100 ℃ of refluxing extraction 20-60min; The filtering separation extracting solution obtains extracting for the first time soup then; Obtain extracting soup for the second time after the purified water that adds 2-5 times of raw material weight in the dregs of a decoction once more repeats to extract, merge 2 times and extract soup;
The extraction soup that (3) will obtain adds 10-30PPM food and mixes at 40-55 ℃ of insulation 3h with polygalacturonase, uses diatomite filtration behind the clarification 24h, removes precipitated impurities, gets the soup clear liquid;
(4) 1/10-9/10 that the soup clear liquid that obtains is concentrated into original volume in vacuum concentration equipment is doubly:
(5) concentrate back soup alcoholic strength to 10%V/V with edible ethanol adjustment, behind the mixing after leaving standstill 20 days under 10 ℃ of conditions spinning clear liquid and deposition, the collecting precipitation material is dried to moisture content and is lower than 13% and is Crude polysaccharides A;
Concentrate back soup alcoholic strength to 20%V/V with edible ethanol adjustment, behind the mixing after leaving standstill 20 days under 10 ℃ of conditions spinning clear liquid and deposition, the collecting precipitation material is dried to moisture content and is lower than 13% and is Crude polysaccharides B;
Concentrate back soup alcoholic strength to 30%V/V with edible ethanol adjustment, behind the mixing after leaving standstill 20 days under 10 ℃ of conditions spinning clear liquid and deposition, the collecting precipitation material is dried to moisture content and is lower than 13% and is Crude polysaccharides C;
Concentrate back soup alcoholic strength to 40%V/V with edible ethanol adjustment, behind the mixing after leaving standstill 20 days under 10 ℃ of conditions spinning clear liquid and deposition, the collecting precipitation material is dried to moisture content and is lower than 13% and is Crude polysaccharides D;
Concentrate back soup alcoholic strength to 50%V/V with edible ethanol adjustment, behind the mixing after leaving standstill 20 days under 10 ℃ of conditions spinning clear liquid and deposition, the collecting precipitation material is dried to moisture content and is lower than 13% and is Crude polysaccharides E;
Concentrate back soup alcoholic strength to 60%V/V with edible ethanol adjustment, behind the mixing after leaving standstill 20 days under 10 ℃ of conditions spinning clear liquid and deposition, the collecting precipitation material is dried to moisture content and is lower than 13% and is Crude polysaccharides F;
Concentrate back soup alcoholic strength to 70%V/V with edible ethanol adjustment, behind the mixing after leaving standstill 20 days under 10 ℃ of conditions spinning clear liquid and deposition, the collecting precipitation material is dried to moisture content and is lower than 13% and is Crude polysaccharides G;
Concentrate back soup alcoholic strength to 80%V/V with edible ethanol adjustment, behind the mixing after leaving standstill 20 days under 10 ℃ of conditions spinning clear liquid and deposition, the collecting precipitation material is dried to moisture content and is lower than 13% and is Crude polysaccharides H.
4. a kind of method with Chinese medicinal materials grading extraction Crude polysaccharides as claimed in claim 1 is characterized in that: the Chinese medicinal materials that wherein contains Crude polysaccharides is wolfberry fruit, Fructus Schisandrae Chinensis, the Radix Astragali or glossy ganoderma.
5. a kind of method with Chinese medicinal materials grading extraction Crude polysaccharides as claimed in claim 1 is characterized in that: wherein edible ethanol concentration is 95%V/V.
6. a kind of method with Chinese medicinal materials grading extraction Crude polysaccharides as claimed in claim 1, it is characterized in that: wherein the drying of sedimentable matter is carried out in vacuum drying oven, drying temperature 50-80 ℃.
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