CN107903328A - A kind of wrinkled papaya seed polysaccharide and preparation method thereof - Google Patents
A kind of wrinkled papaya seed polysaccharide and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a kind of wrinkled papaya seed polysaccharide and preparation method thereof.The pawpaw seed polysaccharide is using the pawpaw seeds dregs of rice by squeezing as raw material, by following steps：A：By the pawpaw seeds dregs of rice drying after squeezing, crush, degreasing is carried out to the pawpaw seeds dregs of rice with nonpolar solvent, obtains degreasing dregs of rice powder；B：Distilled water is added according to certain mass volume ratio to extract at a certain temperature, extracting solution centrifugation removal of impurities, concentration, alcohol precipitation, be dried to obtain pawpaw seeds Thick many candies；C：Pawpaw seeds Thick many candies redissolve, and certain proportion adds Sevage solvents and takes off albumen；D：Polysaccharide solution after de- albumen further isolates and purifies.Polysaccharide yield 2 10% prepared by the method, polyoses content 70 95%.
Description
Technical field
The present invention relates to polysaccharide technical field.
Background technology
Wrinkled papaya is the perennial machaka of rose family Chaenomeles or dungarunga, and there are extensive cultivation in north and south various regions at home
Training, is a kind of important economic tree.The fruits nutrition of wrinkled papaya enriches, fragrant odour contains abundant carbohydrate, saponin(e, has
Machine acid, flavones, vitamin etc., have the special efficacies such as protect liver, anti-oxidant, antibacterial, antitumor, reducing blood lipid.Remember according to Compendium of Materia Medica
Carry, pawpaw have relax channels and collaterals, strengthening the spleen and stomach, benefiting essence-blood pharmacological action, clinically be used for treat vomiting and diarrhoea abdominal pain, rheumatic arthritis,
The diseases such as soreness of waist and knee joint.Wrinkled papaya, which is generally processed, is used as melon dried meat, medicine materical crude slice, papaya wine or papaya ester, has economical well
Value.Skin pawpaw seeds are the processing byproduct of wrinkled papaya, available for squeezing pawpaw seeds oil.The wrinkled papaya seed dregs of rice are pawpaw seeds pressure
Substantial residual thing after having squeezed, the substantial amounts of pawpaw seeds dregs of rice are dropped, and harmful effect are not only caused to environment, and resource does not have yet
To making full use of.
Polysaccharide is the macromolecular polymer that monose is formed by glycosidic bond links, and is widely present in plant, microorganism, algae
In class and animal body.In terms of information interchange of the polysaccharide between cell and cell, cell absorption and immune system molecular recognition
Play and its important effect, therefore also increasingly cause the attention of researcher.In recent years, extract to obtain from natural resources
Some polysaccharide cause the great concern of biochemistry and area of pharmacology.Research shows that polysaccharide has anti-oxidant, antitumor, drop
A variety of physiological activity such as blood fat, anti-aging, antiviral, antiulcer.Wherein, polysaccharide has security in auxiliary for treating cancer
Height, toxic side effect are small, and suppress the advantages that tumor effect is good.Therefore, before the extraction of polysaccharide has a vast market and applies
Scape.
Existing technology discloses the extracting method of a variety of polysaccharide, such as Publication No. CN105820262A and
Enzymatic Extraction polysaccharide is used in the Chinese patent of CN103113489A, the former employs papain and neutral proteinase extraction
Bitter melon polysaccharide, the latter extracts Xinjiang jun dates polysaccharide using biotinylated biomolecule combined-enzyme method, although Enzymatic Extraction polysaccharide mild condition,
The method is complicated, enzyme preparation price is high and enzyme can influence the purity of polysaccharide after inactivation as impurity.Publication No.
In the Chinese patent of CN106589158A, screwtree root polysaccharide is extracted using postcritical method, though this method is easy to operate, is set
Industrialization is difficult to realize for cost is excessive.In the Chinese patent of Publication No. CN103044559A, extracted using Hot water extraction
Red jujube polysaccharide, Extracting temperature are up to 120 DEG C, and extraction time is up to 5h, and the hot environment in this extracting method is easily destroyed polysaccharide
Structure, and the production cycle grow.In the Chinese patent of Publication No. CN103755829A, using inner ebullition decompression method extraction pot
Bottle jujube polysaccharide, this method need to provide condition of negative pressure, to equipment requirement height.
Wrinkled papaya seed total sugar content enriches, but on wrinkled papaya seed polysaccharide preparation method there is not yet related report
Road.
The content of the invention
Surplus economy it is an object of the invention to make full use of the wrinkled papaya seed dregs of rice is worth, there is provided a kind of wrinkled papaya seed
The extracting method of polysaccharide.
To achieve the above object, the present invention uses following steps：
(1) extract
By the pawpaw seeds dregs of rice drying after squeezing, crush, degreasing；Seed dregs of rice distilled water extraction after degreasing, concentration, adds anhydrous second
Alcohol precipitates, and pawpaw seeds Thick many candies are obtained after dry；
(2) separate and purify
A, removing protein；
B, further isolate and purify, obtain the higher pawpaw seed polysaccharide of purity；
B is further isolated and purified, including：
1), dialyse：Pawpaw seeds Thick many candies solution after de- albumen is put into bag filter, is dialysed respectively with tap water, distilled water,
Solution is freeze-dried in bag after dialysis obtains pawpaw seeds two level Thick many candies；
2), ion-exchange chromatography：Pawpaw seeds two level Thick many candies are carried out using 52 cellulose chromatographies of DEAE-cellulose
Isolate and purify, through collecting, being concentrated under reduced pressure, dialysing and being freeze-dried, obtain pawpaw seeds refined polysaccharide；
3), gel filtration chromatography：Pawpaw seeds refined polysaccharide is purified using glucose gel Sephadex G-100, elution,
Collect, concentrate, dialysing, freeze-drying, obtaining the single pawpaw seed polysaccharide of component.
Step (1) is extracted as：By the pawpaw seeds dregs of rice drying after squeezing, crush, by certain mass than 1:10~1:20（g:
ml）, petroleum ether is added, heating and refluxing extraction 1 ~ 2 time at 40 ~ 60 DEG C, when extraction 8 ~ 16 is small every time, filters to obtain filter residue, filter residue
Vacuum dried removing, obtains degreasing pawpaw seeds dregs of rice powder；Extracting degreasing pawpaw seeds dregs of rice powder, by certain mass than 1:10~1:40（g:ml）,
Distilled water is added, stirring or ultrasonic wave assisted extraction or microwave radiation exaraction 1 ~ 3 time, each 20min ~ 4h at 25 ~ 85 DEG C,
Centrifuge and merge supernatant, supernatant is concentrated under reduced pressure at 40 ~ 80 DEG C;It is 1 according to volume ratio:4 (polysaccharide concentrates:Anhydrous second
Alcohol) absolute ethyl alcohol is added into the polysaccharide concentrate after de- albumen, 4 DEG C of overnight alcohol precipitations, are collected by centrifugation precipitation, and repeatedly to precipitation
Thing adds absolute ethyl alcohol and acetone washing；The rotated evaporation of precipitation removing organic solvent completely after washing, adds distilled water
Redissolve, pawpaw seeds Thick many candies are obtained after redissolving solution freeze-drying.
Separation and purifying in step (2)：
A, removing protein：Pawpaw seeds Thick many candies are dissolved in the distilled water of 10 ~ 15 times of quality, obtain the water-soluble of pawpaw seeds Thick many candies
Liquid, reuses Sevage method removing proteins, i.e., chloroform and n-butanol, concentrate, chloroform are added in the aqueous solution of pawpaw seeds Thick many candies
Volume ratio with n-butanol is 25:4:1,10 ~ 20min is shaken, high speed centrifugation, removes organic layer and middle white albumin layer, weight
Multiple extraction occurs up to no white egg white.
During step (2) is separated and purified：1) dialyse：Pawpaw seeds Thick many candies solution after de- albumen is put into molecular cut off
In bag filter for 8000 ~ 12000 Da, respectively with tap water, distilled water dialysis 48h and 24h, solution warp in the bag after dialysis
Freeze-drying obtains pawpaw seeds two level Thick many candies；
During step (2) is separated and purified：2) ion-exchange chromatography：Using 52 cellulose chromatographies pair of DEAE-cellulose
Pawpaw seeds two level Thick many candies are isolated and purified, and weigh a certain amount of pawpaw seeds two level Thick many candies, add the water of 10 ~ 30 times of quality
Dissolving, solution cross 0.45 μm of miillpore filter, gained filtrate 52 cellulose chromatographic columns of upper DEAE-cellulose, with distilled water
And the elution of 0.05mol/L, 0.1mol/L, 0.3mol/L Nacl solution, flow velocity are arranged to 0.8ml/min, automatic fraction collector
Collect, often pipe collects 8.0ml；The outflow of phend-sulphuric acid tracking and monitoring polysaccharide；Using light absorption value as ordinate, elution pipe number is
Abscissa draws elution curve, collects the polysaccharide solution of first and the 4th eluting peak respectively, is concentrated under reduced pressure, distilled water dialysis
48h, freeze-drying obtain pawpaw seeds refined polysaccharide water elution component and Nacl elution fractions.
During step (2) is separated and purified：3) gel filtration chromatography：Using glucose gel Sephadex G-100 to pawpaw seeds
Two components of refined polysaccharide are purified, and take a certain amount of pawpaw seeds refined polysaccharide water elution component and Nacl elution fractions respectively
The water dissolving of 10 ~ 30 times of quality is added, solution crosses 0.45 μm of miillpore filter, the upper glucose gel Sephadex of gained filtrate
G-100 chromatographic columns, using distilled water as eluent, flow velocity is set to 0.3ml/min, and automatic fraction collector is collected, and often pipe is collected
8.0ml；The outflow of phend-sulphuric acid tracking and monitoring polysaccharide；Using light absorption value as ordinate, elution pipe number draws elution for abscissa
Curve, collects first main elution peak and is concentrated under reduced pressure, and distilled water dialysis 48h, freeze-drying obtains single pawpaw seed polysaccharide water
Elution fraction, Nacl elution fractions.
Beneficial effects of the present invention：
1. of the invention easy to operate, condition is easy to control, and purity of polysaccharide is higher；
2. the present invention can turn waste into wealth, the utility value of the wrinkled papaya seed dregs of rice is lifted, improves the comprehensive utilization ratio of wrinkled papaya seed.
Brief description of the drawings
The present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments：
Fig. 1 is 52 column chromatography elution profiles of wrinkled papaya seed polysaccharide DEAE-cellulose of the present invention.
Embodiment
Following embodiments are some citings of the present invention, should not be seen as limitation of the invention.
Embodiment 1
By the pawpaw seeds dregs of rice drying after squeezing, crush, in mass ratio 1:20（g:ml）, petroleum ether is added, is heated at 45 DEG C
Refluxing extraction 2 times, when extraction 10 is small every time, filters to obtain filter residue, the vacuum dried removing of filter residue, obtains degreasing pawpaw seeds dregs of rice powder；Take
Degreasing pawpaw seeds dregs of rice powder, by certain mass than 1:20（g:ml）, distilled water is added, is stirred 2 times, each 2h at 70 DEG C, is centrifuged
And merging supernatant, supernatant is concentrated under reduced pressure at 55 DEG C;It is 1 according to volume ratio:4 (polysaccharide concentrates:Absolute ethyl alcohol) to de-
Absolute ethyl alcohol is added in polysaccharide concentrate after albumen, 4 DEG C of overnight alcohol precipitations, are collected by centrifugation precipitation, and added repeatedly to sediment
Absolute ethyl alcohol and acetone washing；The rotated evaporation of precipitation removing organic solvent completely after washing, adds distilled water redissolution, multiple
Pawpaw seeds Thick many candies are obtained after solution freeze-drying；
Pawpaw seeds Thick many candies are dissolved in the distilled water of 15 times of quality, obtain the aqueous solution of pawpaw seeds Thick many candies, reuse
Add chloroform and n-butanol in Sevage method removing proteins, the i.e. aqueous solution of pawpaw seeds Thick many candies, concentrate, chloroform and n-butanol
Volume ratio is 25:4:1,10min is shaken, high speed centrifugation, removes organic layer and middle white albumin layer, repeats extraction until without white
Chromoprotein layer occurs；
Pawpaw seeds Thick many candies solution after de- albumen is put into the bag filter that molecular cut off is 8000 ~ 12000 Da, respectively
With tap water, distilled water dialysis 48h and 24h, solution is freeze-dried in the bag after dialysis obtains pawpaw seeds two level Thick many candies；
Pawpaw seeds two level Thick many candies are isolated and purified using 52 cellulose chromatographies of DEAE-cellulose, are weighed certain
The pawpaw seeds two level Thick many candies of amount, add the water dissolving of 10 ~ 30 times of quality, and solution crosses 0.45 μm of miillpore filter, gained filtrate
Upper 52 cellulose chromatographic columns of DEAE-cellulose, it is molten with distilled water and 0.05mol/L, 0.1mol/L, 0.3mol/L Nacl
Liquid elutes, and flow velocity is arranged to 0.8ml/min, and automatic fraction collector is collected, and often pipe collects 8.0ml；Phend-sulphuric acid tracking prison
Survey the outflow of polysaccharide；Using light absorption value as ordinate, elution pipe number draws elution curve for abscissa, collects first and the respectively
The polysaccharide solution of four eluting peaks, is concentrated under reduced pressure, and distilled water dialysis 48h, freeze-drying obtains pawpaw seeds refined polysaccharide water elution
Component and Nacl elution fractions；
Two components of pawpaw seeds refined polysaccharide are purified using glucose gel Sephadex G-100, are taken respectively a certain amount of
Pawpaw seeds refined polysaccharide water elution component and Nacl elution fractions add the water dissolvings of 10 ~ 30 times of quality, solution crosses 0.45 μm
Miillpore filter, the upper glucose gel Sephadex G-100 chromatographic columns of gained filtrate, using distilled water as eluent, flow velocity is set to
0.3ml/min, automatic fraction collector are collected, and often pipe collects 8.0ml；The outflow of phend-sulphuric acid tracking and monitoring polysaccharide；To inhale
Light value is ordinate, and elution pipe number draws elution curve for abscissa, collects first main elution peak and is concentrated under reduced pressure, distilled water is saturating
48h is analysed, freeze-drying obtains single pawpaw seed polysaccharide water elution component, Nacl elution fractions.The yield of polysaccharide in this approach
Higher than 3.5%, and the content of phend-sulphuric acid measure polysaccharide, its content are more than 75%.
Embodiment 2
By the pawpaw seeds dregs of rice drying after squeezing, crush, in mass ratio 1:15（g:ml）, petroleum ether is added, is heated at 50 DEG C
Refluxing extraction 2 times, when extraction 12 is small every time, filters to obtain filter residue, the vacuum dried removing of filter residue, obtains degreasing pawpaw seeds dregs of rice powder；Take
Degreasing pawpaw seeds dregs of rice powder, by certain mass than 1:25（g:ml）, distilled water is added, is stirred 2 times, each 2.5h at 80 DEG C, from
The heart simultaneously merges supernatant, and supernatant is concentrated under reduced pressure at 60 DEG C;It is 1 according to volume ratio:4 (polysaccharide concentrates:Absolute ethyl alcohol) to
Absolute ethyl alcohol is added in polysaccharide concentrate after de- albumen, 4 DEG C of overnight alcohol precipitations, are collected by centrifugation precipitation, and add repeatedly to sediment
Enter absolute ethyl alcohol and acetone washing；The rotated evaporation of precipitation removing organic solvent completely after washing, adds distilled water redissolution,
Pawpaw seeds Thick many candies are obtained after redissolving solution freeze-drying；
Pawpaw seeds Thick many candies are dissolved in the distilled water of 10 times of quality, obtain the aqueous solution of pawpaw seeds Thick many candies, reuse
Add chloroform and n-butanol in Sevage method removing proteins, the i.e. aqueous solution of pawpaw seeds Thick many candies, concentrate, chloroform and n-butanol
Volume ratio is 25:4:1,15min is shaken, high speed centrifugation, removes organic layer and middle white albumin layer, repeats extraction until without white
Chromoprotein layer occurs；
Pawpaw seeds Thick many candies solution after de- albumen is put into the bag filter that molecular cut off is 8000 ~ 12000 Da, respectively
With tap water, distilled water dialysis 48h and 24h, solution is freeze-dried in the bag after dialysis obtains pawpaw seeds two level Thick many candies；
Pawpaw seeds two level Thick many candies are isolated and purified using 52 cellulose chromatographies of DEAE-cellulose, are weighed certain
The pawpaw seeds two level Thick many candies of amount, add the water dissolving of 10 ~ 30 times of quality, and solution crosses 0.45 μm of miillpore filter, gained filtrate
Upper 52 cellulose chromatographic columns of DEAE-cellulose, it is molten with distilled water and 0.05mol/L, 0.1mol/L, 0.3mol/L Nacl
Liquid elutes, and flow velocity is arranged to 0.8ml/min, and automatic fraction collector is collected, and often pipe collects 8.0ml；Phend-sulphuric acid tracking prison
Survey the outflow of polysaccharide；Using light absorption value as ordinate, elution pipe number draws elution curve for abscissa, collects first and the respectively
The polysaccharide solution of four eluting peaks, is concentrated under reduced pressure, and distilled water dialysis 48h, freeze-drying obtains pawpaw seeds refined polysaccharide water elution
Component and Nacl elution fractions；
Two components of pawpaw seeds refined polysaccharide are purified using glucose gel Sephadex G-100, are taken respectively a certain amount of
Pawpaw seeds refined polysaccharide water elution component and Nacl elution fractions add the water dissolvings of 10 ~ 30 times of quality, solution crosses 0.45 μm
Miillpore filter, the upper glucose gel Sephadex G-100 chromatographic columns of gained filtrate, using distilled water as eluent, flow velocity is set to
0.3ml/min, automatic fraction collector are collected, and often pipe collects 8.0ml；The outflow of phend-sulphuric acid tracking and monitoring polysaccharide；To inhale
Light value is ordinate, and elution pipe number draws elution curve for abscissa, collects first main elution peak and is concentrated under reduced pressure, distilled water is saturating
48h is analysed, freeze-drying obtains single pawpaw seed polysaccharide water elution component, Nacl elution fractions.The yield of polysaccharide in this approach
Higher than 3.7%, and the content of phend-sulphuric acid measure polysaccharide, its content are more than 78%.
Claims (7)
1. a kind of wrinkled papaya seed polysaccharide and preparation method thereof, its feature exists：
1) extract
By the pawpaw seeds dregs of rice drying after squeezing, crush, degreasing；Seed dregs of rice distilled water extraction after degreasing, concentration, adds anhydrous second
Alcohol precipitates, and pawpaw seeds Thick many candies are obtained after dry；
2) separate and purify
A. removing protein；B. further isolate and purify, obtain the higher pawpaw seed polysaccharide of purity.
2. a kind of wrinkled papaya seed polysaccharide according to claim 1 and preparation method thereof, it is characterised in that the b is into one
Step separation and purifying, including：
1), dialyse：Pawpaw seeds Thick many candies solution after de- albumen is put into bag filter, is dialysed respectively with tap water, distilled water,
Solution is freeze-dried in bag after dialysis obtains pawpaw seeds two level Thick many candies；
2), ion-exchange chromatography：Pawpaw seeds two level Thick many candies are carried out using 52 cellulose chromatographies of DEAE-cellulose
Isolate and purify, through collecting, being concentrated under reduced pressure, dialysing and being freeze-dried, obtain pawpaw seeds refined polysaccharide；
3), gel filtration chromatography：Pawpaw seeds refined polysaccharide is purified using glucose gel Sephadex G-100, elution,
Collect, concentrate, dialysing, freeze-drying, obtaining the single pawpaw seed polysaccharide of component.
3. a kind of wrinkled papaya seed polysaccharide according to claim 1 and preparation method thereof, its feature exists, step
(1) it is extracted as：By the pawpaw seeds dregs of rice drying after squeezing, crush, by certain mass than 1:10~1:20（g:ml）, add
Petroleum ether, heating and refluxing extraction 2 ~ 4 times at 40 ~ 60 DEG C, when extraction 8 ~ 16 is small every time, filter to obtain filter residue, filter residue is done through vacuum
Dry removing solvent, obtains degreasing pawpaw seeds dregs of rice powder；Extracting degreasing pawpaw seeds dregs of rice powder, by certain mass than 1:10~1:40（g:ml）, add
Distilled water, stirring extraction 1 ~ 3 time, each 20min ~ 4h, centrifuges and merges supernatant, supernatant is in 40 ~ 80 at 25 ~ 85 DEG C
It is concentrated under reduced pressure at DEG C;It is 1 according to volume ratio:4 (polysaccharide concentrates:Absolute ethyl alcohol) added into the polysaccharide concentrate after de- albumen
Absolute ethyl alcohol, 4 DEG C of overnight alcohol precipitations, are collected by centrifugation precipitation, and add absolute ethyl alcohol and acetone washing to sediment repeatedly；After washing
Precipitation it is rotated evaporation completely removing organic solvent, add distilled water redissolution, redissolve solution freeze-drying after obtain pawpaw
Seed Thick many candies.
4. a kind of wrinkled papaya seed polysaccharide according to claim 1 and preparation method thereof, its feature exists, step
(2) in separating and purify：
A, removing protein is:Pawpaw seeds Thick many candies are dissolved in the distilled water of 10 ~ 15 times of quality, obtain the water-soluble of pawpaw seeds Thick many candies
Liquid, reuses Sevage method removing proteins, i.e., chloroform and n-butanol, pawpaw seeds Thick many candies are added in the aqueous solution of pawpaw seeds Thick many candies
The volume ratio of aqueous solution, chloroform and n-butanol is 25:4:1,10 ~ 20min is shaken, high speed centrifugation, removes organic layer and centre is white
Chromoprotein layer, repeats extraction up to no white egg white occurs.
5. a kind of wrinkled papaya seed polysaccharide according to claim 2 and preparation method thereof, its feature exists, described further to divide
From with purifying:1) dialyse：It is 8000 ~ 12000 Da's that pawpaw seeds Thick many candies solution after de- albumen is put into molecular cut off
In bag filter, respectively with tap water, distilled water dialysis 48h and 24h, solution is freeze-dried in the bag after dialysis obtains pawpaw seeds
Two level Thick many candies.
6. a kind of wrinkled papaya seed polysaccharide according to claim 2 and preparation method thereof, its feature exists, described further to divide
From with purifying:2) ion-exchange chromatography：It is slightly more to pawpaw seeds two level using 52 cellulose chromatographies of DEAE-cellulose
Sugar is isolated and purified, and weighs a certain amount of pawpaw seeds two level Thick many candies, adds the water dissolving of 10 ~ 30 times of quality, solution mistake
0.45 μm of miillpore filter, the upper 52 cellulose chromatographic columns of DEAE-cellulose of gained filtrate, with distilled water and 0.05mol/L,
0.1mol/L, the elution of 0.3mol/L Nacl solution, flow velocity are arranged to 0.8ml/min, and automatic fraction collector is collected, and often pipe is received
Collect 8.0ml；The outflow of phend-sulphuric acid tracking and monitoring polysaccharide；Using light absorption value as ordinate, elution pipe number is drawn for abscissa and is washed
De- curve, collects the polysaccharide solution of first and the 4th eluting peak, is concentrated under reduced pressure respectively, distilled water dialysis 48h, freeze-drying
Obtain pawpaw seeds refined polysaccharide water elution component and Nacl elution fractions.
7. a kind of wrinkled papaya seed polysaccharide according to claim 2 and preparation method thereof, its feature exists, described further to divide
From with purifying:3) gel filtration chromatography：Using glucose gel Sephadex G-100 to two components of pawpaw seeds refined polysaccharide
Purified, take a certain amount of pawpaw seeds refined polysaccharide water elution component and Nacl elution fractions to add 10 ~ 30 times of quality respectively
Water dissolving, solution crosses 0.45 μm of miillpore filter, the upper glucose gel Sephadex G-100 chromatographic columns of gained filtrate, to steam
Distilled water is eluent, and flow velocity is set to 0.3ml/min, and automatic fraction collector is collected, and often pipe collects 8.0ml；Phend-sulphuric acid with
Track monitors the outflow of polysaccharide；Using light absorption value as ordinate, elution pipe number draws elution curve for abscissa, collects first elution
Main peak is concentrated under reduced pressure, and distilled water dialysis 48h, freeze-drying obtains single pawpaw seed polysaccharide water elution component, Nacl elution groups
Point.
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| CN110128559A (en) * | 2019-04-29 | 2019-08-16 | 华南农业大学 | A kind of preparation method and applications with immunoregulatory fire ginseng fruit pericarp polysaccharide |
| CN110128559B (en) * | 2019-04-29 | 2021-02-19 | 华南农业大学 | Preparation method and application of polysaccharide of fruit peel of fire ginseng with immunoregulation function |
| CN112753927A (en) * | 2021-01-25 | 2021-05-07 | 陕西师范大学 | Fruit juice bacteriostatic agent based on neutral polysaccharide of lentinus edodes stems |
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