CN103270172B - 血清中二噁英类的新生物检测方法及其在代谢综合征和相关病症中的诊断用途 - Google Patents

血清中二噁英类的新生物检测方法及其在代谢综合征和相关病症中的诊断用途 Download PDF

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CN103270172B
CN103270172B CN201180053287.9A CN201180053287A CN103270172B CN 103270172 B CN103270172 B CN 103270172B CN 201180053287 A CN201180053287 A CN 201180053287A CN 103270172 B CN103270172 B CN 103270172B
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dioxin
serum
promotor
reporter gene
gene
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CN103270172A (zh
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李弘揆
金永美
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Industry Academic Cooperation Foundation of Kyung Hee University
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • C—CHEMISTRY; METALLURGY
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5067—Liver cells
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    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C12N2015/8527—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic for producing animal models, e.g. for tests or diseases
    • C12N2015/8572—Animal models for proliferative diseases, e.g. comprising an oncogene
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/04—Endocrine or metabolic disorders

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  • Health & Medical Sciences (AREA)
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  • Wood Science & Technology (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201180053287.9A 2010-09-10 2011-09-06 血清中二噁英类的新生物检测方法及其在代谢综合征和相关病症中的诊断用途 Active CN103270172B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR1020100088905A KR101272315B1 (ko) 2010-09-10 2010-09-10 새로운 혈청 내 다이옥신류의 생물학적 검출 방법, 및 이의 대사증후군 및 관련 증상에 대한 진단적 유용성
KR10-2010-0088905 2010-09-10
PCT/KR2011/006583 WO2012033324A2 (ko) 2010-09-10 2011-09-06 새로운 혈청 내 다이옥신류의 생물학적 검출 방법, 및 이의 대사증후군 및 관련 증상에 대한 진단적 유용성

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CN103270172A CN103270172A (zh) 2013-08-28
CN103270172B true CN103270172B (zh) 2016-03-16

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US (3) US20130171655A1 (ko)
EP (1) EP2615179B1 (ko)
JP (1) JP5736458B2 (ko)
KR (1) KR101272315B1 (ko)
CN (1) CN103270172B (ko)
DK (1) DK2615179T3 (ko)
HR (1) HRP20171436T1 (ko)
SI (1) SI2615179T1 (ko)
WO (1) WO2012033324A2 (ko)

Families Citing this family (4)

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Publication number Priority date Publication date Assignee Title
KR101272315B1 (ko) 2010-09-10 2013-06-07 경희대학교 산학협력단 새로운 혈청 내 다이옥신류의 생물학적 검출 방법, 및 이의 대사증후군 및 관련 증상에 대한 진단적 유용성
CN109306001A (zh) * 2018-11-21 2019-02-05 浙江海洋大学 芳香烃受体核转位蛋白arnt——一种新型海洋生物污染检测标志物
CN109813913B (zh) * 2019-01-31 2021-11-09 中国医学科学院肿瘤医院 芳烃受体(AhR)在预测免疫治疗效果中的应用
CN114459859B (zh) * 2022-02-16 2022-09-09 北京卢米斯生物科技有限公司 基于荧光素酶报告基因法的高通量二噁英自动检测装置

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20010046920A (ko) * 1999-11-16 2001-06-15 정명식 다이옥신류에 의하여 발현이 조절되는 재조합 리포터유전자를 가지는 형질전환 세포주를 이용한 다이옥신류의생물학적 분석방법
US6432692B1 (en) * 1999-06-21 2002-08-13 Wisconsin Alumni Research Foundation Sensitive bioassay for detecting agonists of the aryl hydrocarbon receptor
EP1767622A1 (en) * 2004-05-24 2007-03-28 Yamanashi University Plasmid responsive to dioxin, etc., transgenic cell for assay of dioxin, etc. and utilizing the same, dioxin, etc. detecting method and biosensor

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5854010A (en) * 1997-03-10 1998-12-29 Denison; Michael S. Bioassay for detecting 2,3,7,8-tetrachlorodibenzo-para-dioxin and TCDD-like compounds and novel recombinant cell line useful therefor
JP2006254714A (ja) * 2005-03-15 2006-09-28 Sumitomo Chemical Co Ltd Ａｈレセプター転写促進能増強細胞及びその利用
JPWO2007004361A1 (ja) 2005-07-01 2009-01-22 国立大学法人山梨大学 遺伝子導入非ヒト哺乳動物、およびこれを用いた環境中有害化学物質のモニタリング方法
KR101272315B1 (ko) 2010-09-10 2013-06-07 경희대학교 산학협력단 새로운 혈청 내 다이옥신류의 생물학적 검출 방법, 및 이의 대사증후군 및 관련 증상에 대한 진단적 유용성

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6432692B1 (en) * 1999-06-21 2002-08-13 Wisconsin Alumni Research Foundation Sensitive bioassay for detecting agonists of the aryl hydrocarbon receptor
KR20010046920A (ko) * 1999-11-16 2001-06-15 정명식 다이옥신류에 의하여 발현이 조절되는 재조합 리포터유전자를 가지는 형질전환 세포주를 이용한 다이옥신류의생물학적 분석방법
EP1767622A1 (en) * 2004-05-24 2007-03-28 Yamanashi University Plasmid responsive to dioxin, etc., transgenic cell for assay of dioxin, etc. and utilizing the same, dioxin, etc. detecting method and biosensor

Also Published As

Publication number Publication date
KR101272315B1 (ko) 2013-06-07
CN103270172A (zh) 2013-08-28
WO2012033324A3 (ko) 2012-06-14
JP5736458B2 (ja) 2015-06-17
SI2615179T1 (sl) 2017-12-29
US20190309376A1 (en) 2019-10-10
HRP20171436T1 (hr) 2017-11-03
US20130171655A1 (en) 2013-07-04
EP2615179A4 (en) 2014-03-19
EP2615179A2 (en) 2013-07-17
EP2615179B1 (en) 2017-08-02
DK2615179T3 (da) 2017-11-13
JP2013540433A (ja) 2013-11-07
WO2012033324A2 (ko) 2012-03-15
US20170107584A1 (en) 2017-04-20
KR20120026788A (ko) 2012-03-20
US10697027B2 (en) 2020-06-30

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