WO2012033324A3 - 새로운 혈청 내 다이옥신류의 생물학적 검출 방법, 및 이의 대사증후군 및 관련 증상에 대한 진단적 유용성 - Google Patents

새로운 혈청 내 다이옥신류의 생물학적 검출 방법, 및 이의 대사증후군 및 관련 증상에 대한 진단적 유용성 Download PDF

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WO2012033324A3
WO2012033324A3 PCT/KR2011/006583 KR2011006583W WO2012033324A3 WO 2012033324 A3 WO2012033324 A3 WO 2012033324A3 KR 2011006583 W KR2011006583 W KR 2011006583W WO 2012033324 A3 WO2012033324 A3 WO 2012033324A3
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serum
dioxins
biological detection
dioxin
metabolic syndrome
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PCT/KR2011/006583
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English (en)
French (fr)
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WO2012033324A2 (ko
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이홍규
김영미
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경희대학교 산학협력단
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Priority to SI201131306T priority Critical patent/SI2615179T1/sl
Priority to EP11823757.7A priority patent/EP2615179B1/en
Priority to US13/821,779 priority patent/US20130171655A1/en
Priority to CN201180053287.9A priority patent/CN103270172B/zh
Priority to JP2013528112A priority patent/JP5736458B2/ja
Priority to DK11823757.7T priority patent/DK2615179T3/da
Publication of WO2012033324A2 publication Critical patent/WO2012033324A2/ko
Publication of WO2012033324A3 publication Critical patent/WO2012033324A3/ko
Priority to US15/051,385 priority patent/US20170107584A1/en
Priority to HRP20171436TT priority patent/HRP20171436T1/hr
Priority to US16/417,523 priority patent/US10697027B2/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5067—Liver cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C12N2015/8527—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic for producing animal models, e.g. for tests or diseases
    • C12N2015/8572—Animal models for proliferative diseases, e.g. comprising an oncogene
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/04—Endocrine or metabolic disorders

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  • Health & Medical Sciences (AREA)
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  • Chemical & Material Sciences (AREA)
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  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

본 발명은 새로운 혈청 내 다이옥신류의 생물학적 검출 방법, 및 이의 대사증후군 및 관련 증상에 대한 진단적 유용성에 관한 것으로서, 구체적으로 다이옥신 반응 요소(dioxin responsive element), 프로모터, 및 리포터 유전자가 작동가능하게 연결된 유전자 컨스트럭트를 포함하는 재조합 벡터로 형질전환된 세포와 혈청 전체를 사용함으로써, 루시퍼라제 활성을 측정하여 혈청 내 다이옥신 함량과 신체 변수들 간의 유의적인 상관성을 확인하였고, 혈청으로부터 다이옥신을 정제하기 위한 전처리 과정이 요구되는 기존의 방법으로부터 개선되어, 혈청 전체를 사용함에 따라 다수의 시료를 극소량으로도 쉽고 정확하게 분석할 수 있으므로, 다이옥신류 화합물에 의하여 발현이 조절되는 재조합 리포터 유전자를 포함하는 형질전환 세포주에 전체 혈청(total serum)을 처리하는 본 발명의 검출 시스템은 혈청 내 다이옥신의 생물학적 검출에 유용하게 이용할 수 있고, 이러한 방법을 사용하여 혈청 내 존재하는 다이옥신과 같은 POPs의 여부를 정확히 검출함으로써, 특정 POPs와 환자의 질병인자 간의 상관성을 연구하고, 이를 질병의 발병 예측 및 치료 여부를 판정하는데 유용하게 사용할 수 있다.
PCT/KR2011/006583 2010-09-10 2011-09-06 새로운 혈청 내 다이옥신류의 생물학적 검출 방법, 및 이의 대사증후군 및 관련 증상에 대한 진단적 유용성 WO2012033324A2 (ko)

Priority Applications (9)

Application Number Priority Date Filing Date Title
SI201131306T SI2615179T1 (sl) 2010-09-10 2011-09-06 Novi biološki detekcijski postopek za dioksine v serumu in njegova diagnostnična uporaba za metabolični sindrom in sorodna stanja
EP11823757.7A EP2615179B1 (en) 2010-09-10 2011-09-06 Novel biological detection method for dioxins in serum, and a diagnostic use therefor in metabolic syndrome and related conditions
US13/821,779 US20130171655A1 (en) 2010-09-10 2011-09-06 Novel biological detection method for dioxins in serum, and a diagnostic use therefor in metabolic syndrome and related conditions
CN201180053287.9A CN103270172B (zh) 2010-09-10 2011-09-06 血清中二噁英类的新生物检测方法及其在代谢综合征和相关病症中的诊断用途
JP2013528112A JP5736458B2 (ja) 2010-09-10 2011-09-06 血清内ダイオキシン類の新規生物学的検出方法、及びその代謝症候群及び関連症状に対する診断的使用
DK11823757.7T DK2615179T3 (da) 2010-09-10 2011-09-06 Hidtil ukendt biologisk påvisningsfremgangsmåde til dioxiner i serum, og en diagnostisk anvendelse derfor i metabolisk syndrom og beslægtede tilstande
US15/051,385 US20170107584A1 (en) 2010-09-10 2016-02-23 Novel biological detection method for dioxins in serum, and a diagnostic use therefor in metabolic syndrome and related conditions
HRP20171436TT HRP20171436T1 (hr) 2010-09-10 2017-09-22 Novi biološki postupak detektiranja dioksina u serumu i njegova dijagnostička upotreba kod metaboličkog sindroma i srodnih stanja
US16/417,523 US10697027B2 (en) 2010-09-10 2019-05-20 Biological detection method for dioxins in serum, and diagnostic use therefor in metabolic syndrome and related conditions

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020100088905A KR101272315B1 (ko) 2010-09-10 2010-09-10 새로운 혈청 내 다이옥신류의 생물학적 검출 방법, 및 이의 대사증후군 및 관련 증상에 대한 진단적 유용성
KR10-2010-0088905 2010-09-10

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US13/821,779 A-371-Of-International US20130171655A1 (en) 2010-09-10 2011-09-06 Novel biological detection method for dioxins in serum, and a diagnostic use therefor in metabolic syndrome and related conditions
US15/051,385 Division US20170107584A1 (en) 2010-09-10 2016-02-23 Novel biological detection method for dioxins in serum, and a diagnostic use therefor in metabolic syndrome and related conditions

Publications (2)

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WO2012033324A2 WO2012033324A2 (ko) 2012-03-15
WO2012033324A3 true WO2012033324A3 (ko) 2012-06-14

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PCT/KR2011/006583 WO2012033324A2 (ko) 2010-09-10 2011-09-06 새로운 혈청 내 다이옥신류의 생물학적 검출 방법, 및 이의 대사증후군 및 관련 증상에 대한 진단적 유용성

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US (3) US20130171655A1 (ko)
EP (1) EP2615179B1 (ko)
JP (1) JP5736458B2 (ko)
KR (1) KR101272315B1 (ko)
CN (1) CN103270172B (ko)
DK (1) DK2615179T3 (ko)
HR (1) HRP20171436T1 (ko)
SI (1) SI2615179T1 (ko)
WO (1) WO2012033324A2 (ko)

Families Citing this family (4)

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Publication number Priority date Publication date Assignee Title
KR101272315B1 (ko) 2010-09-10 2013-06-07 경희대학교 산학협력단 새로운 혈청 내 다이옥신류의 생물학적 검출 방법, 및 이의 대사증후군 및 관련 증상에 대한 진단적 유용성
CN109306001A (zh) * 2018-11-21 2019-02-05 浙江海洋大学 芳香烃受体核转位蛋白arnt——一种新型海洋生物污染检测标志物
CN109813913B (zh) * 2019-01-31 2021-11-09 中国医学科学院肿瘤医院 芳烃受体(AhR)在预测免疫治疗效果中的应用
CN114459859B (zh) * 2022-02-16 2022-09-09 北京卢米斯生物科技有限公司 基于荧光素酶报告基因法的高通量二噁英自动检测装置

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5854010A (en) * 1997-03-10 1998-12-29 Denison; Michael S. Bioassay for detecting 2,3,7,8-tetrachlorodibenzo-para-dioxin and TCDD-like compounds and novel recombinant cell line useful therefor
KR20010046920A (ko) * 1999-11-16 2001-06-15 정명식 다이옥신류에 의하여 발현이 조절되는 재조합 리포터유전자를 가지는 형질전환 세포주를 이용한 다이옥신류의생물학적 분석방법

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6432692B1 (en) * 1999-06-21 2002-08-13 Wisconsin Alumni Research Foundation Sensitive bioassay for detecting agonists of the aryl hydrocarbon receptor
JP4815602B2 (ja) * 2004-05-24 2011-11-16 国立大学法人山梨大学 ダイオキシン類等応答性プラスミド、ダイオキシン類等測定用遺伝子導入細胞、並びにそれを用いたダイオキシン類等検出方法及びバイオセンサー
JP2006254714A (ja) * 2005-03-15 2006-09-28 Sumitomo Chemical Co Ltd Ａｈレセプター転写促進能増強細胞及びその利用
JPWO2007004361A1 (ja) 2005-07-01 2009-01-22 国立大学法人山梨大学 遺伝子導入非ヒト哺乳動物、およびこれを用いた環境中有害化学物質のモニタリング方法
KR101272315B1 (ko) 2010-09-10 2013-06-07 경희대학교 산학협력단 새로운 혈청 내 다이옥신류의 생물학적 검출 방법, 및 이의 대사증후군 및 관련 증상에 대한 진단적 유용성

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5854010A (en) * 1997-03-10 1998-12-29 Denison; Michael S. Bioassay for detecting 2,3,7,8-tetrachlorodibenzo-para-dioxin and TCDD-like compounds and novel recombinant cell line useful therefor
KR20010046920A (ko) * 1999-11-16 2001-06-15 정명식 다이옥신류에 의하여 발현이 조절되는 재조합 리포터유전자를 가지는 형질전환 세포주를 이용한 다이옥신류의생물학적 분석방법

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
MURK A.J. ET AL.: "The CALUX (Chemical-activated luciferase expression) assay adapted and validated for measuring TCDD equivalents in blood plasma", ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, vol. 16, no. 8, August 1997 (1997-08-01), pages 1583 - 1589, XP055096725 *
VONDRACEK J. ET AL.: "Monitoring river sediments contaminated predominantly with polyaromatic hydrocarbons by chemical and in vitro bioassay techniques", ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, vol. 20, no. 7, July 2001 (2001-07-01), pages 1499 - 1506, XP055096727 *

Also Published As

Publication number Publication date
EP2615179A4 (en) 2014-03-19
CN103270172A (zh) 2013-08-28
US20170107584A1 (en) 2017-04-20
SI2615179T1 (sl) 2017-12-29
CN103270172B (zh) 2016-03-16
US20190309376A1 (en) 2019-10-10
JP2013540433A (ja) 2013-11-07
JP5736458B2 (ja) 2015-06-17
DK2615179T3 (da) 2017-11-13
US20130171655A1 (en) 2013-07-04
WO2012033324A2 (ko) 2012-03-15
EP2615179B1 (en) 2017-08-02
KR20120026788A (ko) 2012-03-20
EP2615179A2 (en) 2013-07-17
HRP20171436T1 (hr) 2017-11-03
KR101272315B1 (ko) 2013-06-07
US10697027B2 (en) 2020-06-30

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