CN103263660A - Earthworm protein for reducing blood sugar and blood pressure and eliminating diabetic complications and application pf earthworm protein - Google Patents
Earthworm protein for reducing blood sugar and blood pressure and eliminating diabetic complications and application pf earthworm protein Download PDFInfo
- Publication number
- CN103263660A CN103263660A CN2013101739035A CN201310173903A CN103263660A CN 103263660 A CN103263660 A CN 103263660A CN 2013101739035 A CN2013101739035 A CN 2013101739035A CN 201310173903 A CN201310173903 A CN 201310173903A CN 103263660 A CN103263660 A CN 103263660A
- Authority
- CN
- China
- Prior art keywords
- powder
- lumbricus
- earthworm protein
- blood
- parts
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000361919 Metaphire sieboldi Species 0.000 title claims abstract description 134
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 121
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 121
- 210000004369 blood Anatomy 0.000 title claims abstract description 108
- 239000008280 blood Substances 0.000 title claims abstract description 108
- 230000036772 blood pressure Effects 0.000 title claims abstract description 22
- 208000002249 Diabetes Complications Diseases 0.000 title claims abstract description 17
- 206010012655 Diabetic complications Diseases 0.000 title claims abstract description 16
- 230000000694 effects Effects 0.000 claims abstract description 62
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 40
- 238000003756 stirring Methods 0.000 claims abstract description 26
- 239000012535 impurity Substances 0.000 claims abstract description 23
- 238000001914 filtration Methods 0.000 claims abstract description 18
- 102000004190 Enzymes Human genes 0.000 claims abstract description 16
- 108090000790 Enzymes Proteins 0.000 claims abstract description 16
- 241001233061 earthworms Species 0.000 claims abstract description 16
- 238000004140 cleaning Methods 0.000 claims abstract description 12
- 238000001035 drying Methods 0.000 claims abstract description 12
- 238000004108 freeze drying Methods 0.000 claims abstract description 6
- 239000000843 powder Substances 0.000 claims description 141
- 241000243684 Lumbricus Species 0.000 claims description 116
- 230000004089 microcirculation Effects 0.000 claims description 53
- 230000006872 improvement Effects 0.000 claims description 50
- 241000689272 Senna sophera Species 0.000 claims description 49
- 230000008030 elimination Effects 0.000 claims description 47
- 238000003379 elimination reaction Methods 0.000 claims description 47
- 239000003814 drug Substances 0.000 claims description 44
- 240000004507 Abelmoschus esculentus Species 0.000 claims description 32
- 235000003934 Abelmoschus esculentus Nutrition 0.000 claims description 32
- 239000007788 liquid Substances 0.000 claims description 32
- 235000014101 wine Nutrition 0.000 claims description 31
- 230000036541 health Effects 0.000 claims description 30
- 239000000047 product Substances 0.000 claims description 29
- 239000002994 raw material Substances 0.000 claims description 28
- 238000011282 treatment Methods 0.000 claims description 28
- 235000013339 cereals Nutrition 0.000 claims description 25
- 238000012545 processing Methods 0.000 claims description 25
- 238000005406 washing Methods 0.000 claims description 20
- 206010012601 diabetes mellitus Diseases 0.000 claims description 18
- 239000002131 composite material Substances 0.000 claims description 16
- 239000006228 supernatant Substances 0.000 claims description 15
- 238000004587 chromatography analysis Methods 0.000 claims description 13
- 240000001008 Dimocarpus longan Species 0.000 claims description 12
- 235000000235 Euphoria longan Nutrition 0.000 claims description 12
- 230000001737 promoting effect Effects 0.000 claims description 12
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 11
- 241001614060 Amynthas aspergillus Species 0.000 claims description 10
- 241001006778 Amythas Species 0.000 claims description 10
- 101100381997 Danio rerio tbc1d32 gene Proteins 0.000 claims description 10
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 10
- 241000912926 Metaphire tschiliensis Species 0.000 claims description 10
- 101100381999 Mus musculus Tbc1d32 gene Proteins 0.000 claims description 10
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 claims description 10
- 239000012153 distilled water Substances 0.000 claims description 10
- 238000005286 illumination Methods 0.000 claims description 10
- 238000002360 preparation method Methods 0.000 claims description 10
- 235000020095 red wine Nutrition 0.000 claims description 10
- 239000002893 slag Substances 0.000 claims description 10
- 239000001509 sodium citrate Substances 0.000 claims description 10
- 235000011083 sodium citrates Nutrition 0.000 claims description 10
- 230000001954 sterilising effect Effects 0.000 claims description 10
- 238000004659 sterilization and disinfection Methods 0.000 claims description 10
- 239000011550 stock solution Substances 0.000 claims description 10
- 238000000108 ultra-filtration Methods 0.000 claims description 10
- 238000010792 warming Methods 0.000 claims description 10
- 239000004475 Arginine Substances 0.000 claims description 9
- 206010029164 Nephrotic syndrome Diseases 0.000 claims description 9
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims description 9
- 230000002265 prevention Effects 0.000 claims description 8
- 239000000243 solution Substances 0.000 claims description 8
- 244000281702 Dioscorea villosa Species 0.000 claims description 7
- 235000004879 dioscorea Nutrition 0.000 claims description 7
- 235000013305 food Nutrition 0.000 claims description 7
- 210000000582 semen Anatomy 0.000 claims description 7
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 claims description 6
- LUEWUZLMQUOBSB-FSKGGBMCSA-N (2s,3s,4s,5s,6r)-2-[(2r,3s,4r,5r,6s)-6-[(2r,3s,4r,5s,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5s,6r)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-4,5-dihydroxy-2-(hydroxymethyl)oxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@@H](O[C@@H]2[C@H](O[C@@H](OC3[C@H](O[C@@H](O)[C@@H](O)[C@H]3O)CO)[C@@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O LUEWUZLMQUOBSB-FSKGGBMCSA-N 0.000 claims description 5
- 241000190633 Cordyceps Species 0.000 claims description 5
- 235000005903 Dioscorea Nutrition 0.000 claims description 5
- 235000000504 Dioscorea villosa Nutrition 0.000 claims description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 5
- 229920002581 Glucomannan Polymers 0.000 claims description 5
- 241000545442 Radix Species 0.000 claims description 5
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 5
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 5
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 5
- 238000005571 anion exchange chromatography Methods 0.000 claims description 5
- 238000011109 contamination Methods 0.000 claims description 5
- 230000003247 decreasing effect Effects 0.000 claims description 5
- 238000007598 dipping method Methods 0.000 claims description 5
- 238000007710 freezing Methods 0.000 claims description 5
- 230000008014 freezing Effects 0.000 claims description 5
- 229940046240 glucomannan Drugs 0.000 claims description 5
- 238000000227 grinding Methods 0.000 claims description 5
- 238000000703 high-speed centrifugation Methods 0.000 claims description 5
- 238000001802 infusion Methods 0.000 claims description 5
- 239000012528 membrane Substances 0.000 claims description 5
- 238000005374 membrane filtration Methods 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
- 230000000474 nursing effect Effects 0.000 claims description 5
- 239000003208 petroleum Substances 0.000 claims description 5
- 239000011148 porous material Substances 0.000 claims description 5
- 239000002244 precipitate Substances 0.000 claims description 5
- 238000010298 pulverizing process Methods 0.000 claims description 5
- 229920006395 saturated elastomer Polymers 0.000 claims description 5
- 238000012216 screening Methods 0.000 claims description 5
- 238000004062 sedimentation Methods 0.000 claims description 5
- 238000002791 soaking Methods 0.000 claims description 5
- 239000011780 sodium chloride Substances 0.000 claims description 5
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims description 5
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical class [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 claims description 5
- 238000001291 vacuum drying Methods 0.000 claims description 5
- 230000002829 reductive effect Effects 0.000 abstract description 3
- 238000002386 leaching Methods 0.000 abstract description 2
- 150000003839 salts Chemical class 0.000 abstract description 2
- 238000011010 flushing procedure Methods 0.000 abstract 1
- 238000004537 pulping Methods 0.000 abstract 1
- 235000018102 proteins Nutrition 0.000 description 104
- 241000237636 Pheretima Species 0.000 description 20
- 208000007536 Thrombosis Diseases 0.000 description 19
- 238000012360 testing method Methods 0.000 description 15
- 239000003146 anticoagulant agent Substances 0.000 description 12
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 12
- 229940088598 enzyme Drugs 0.000 description 12
- 239000008103 glucose Substances 0.000 description 11
- 102000015779 HDL Lipoproteins Human genes 0.000 description 10
- 108010010234 HDL Lipoproteins Proteins 0.000 description 10
- 210000004027 cell Anatomy 0.000 description 10
- 230000007423 decrease Effects 0.000 description 10
- 230000006870 function Effects 0.000 description 10
- 210000004204 blood vessel Anatomy 0.000 description 9
- 239000007789 gas Substances 0.000 description 9
- 239000000203 mixture Substances 0.000 description 9
- 230000002537 thrombolytic effect Effects 0.000 description 9
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 8
- 235000009697 arginine Nutrition 0.000 description 8
- 201000010099 disease Diseases 0.000 description 8
- 239000000284 extract Substances 0.000 description 8
- 235000019197 fats Nutrition 0.000 description 8
- 230000000630 rising effect Effects 0.000 description 8
- 206010020772 Hypertension Diseases 0.000 description 7
- 229940079593 drug Drugs 0.000 description 7
- 210000000952 spleen Anatomy 0.000 description 7
- 241001465754 Metazoa Species 0.000 description 6
- 206010028980 Neoplasm Diseases 0.000 description 6
- 239000011543 agarose gel Substances 0.000 description 6
- 235000001014 amino acid Nutrition 0.000 description 6
- 150000001413 amino acids Chemical class 0.000 description 6
- 230000023555 blood coagulation Effects 0.000 description 6
- 210000003734 kidney Anatomy 0.000 description 6
- 230000000144 pharmacologic effect Effects 0.000 description 6
- 210000002381 plasma Anatomy 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 102000009123 Fibrin Human genes 0.000 description 5
- 108010073385 Fibrin Proteins 0.000 description 5
- 229940127219 anticoagulant drug Drugs 0.000 description 5
- 230000009286 beneficial effect Effects 0.000 description 5
- 230000017531 blood circulation Effects 0.000 description 5
- 238000004440 column chromatography Methods 0.000 description 5
- 239000004615 ingredient Substances 0.000 description 5
- BWGVNKXGVNDBDI-UHFFFAOYSA-N Fibrin monomer Chemical compound CNC(=O)CNC(=O)CN BWGVNKXGVNDBDI-UHFFFAOYSA-N 0.000 description 4
- 108010088842 Fibrinolysin Proteins 0.000 description 4
- 101000622137 Homo sapiens P-selectin Proteins 0.000 description 4
- 241000699670 Mus sp. Species 0.000 description 4
- 102100023472 P-selectin Human genes 0.000 description 4
- 229930003427 Vitamin E Natural products 0.000 description 4
- 230000004913 activation Effects 0.000 description 4
- 210000001736 capillary Anatomy 0.000 description 4
- 208000026106 cerebrovascular disease Diseases 0.000 description 4
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 4
- 230000002526 effect on cardiovascular system Effects 0.000 description 4
- 229950003499 fibrin Drugs 0.000 description 4
- 229940001501 fibrinolysin Drugs 0.000 description 4
- 239000003527 fibrinolytic agent Substances 0.000 description 4
- 230000003480 fibrinolytic effect Effects 0.000 description 4
- IXZISFNWUWKBOM-ARQDHWQXSA-N fructosamine Chemical compound NC[C@@]1(O)OC[C@@H](O)[C@@H](O)[C@@H]1O IXZISFNWUWKBOM-ARQDHWQXSA-N 0.000 description 4
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 4
- 230000003345 hyperglycaemic effect Effects 0.000 description 4
- FDGQSTZJBFJUBT-UHFFFAOYSA-N hypoxanthine Chemical compound O=C1NC=NC2=C1NC=N2 FDGQSTZJBFJUBT-UHFFFAOYSA-N 0.000 description 4
- 230000036039 immunity Effects 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 230000003014 reinforcing effect Effects 0.000 description 4
- 231100000027 toxicology Toxicity 0.000 description 4
- 239000011573 trace mineral Substances 0.000 description 4
- 235000013619 trace mineral Nutrition 0.000 description 4
- 235000019165 vitamin E Nutrition 0.000 description 4
- 229940046009 vitamin E Drugs 0.000 description 4
- 239000011709 vitamin E Substances 0.000 description 4
- 208000024172 Cardiovascular disease Diseases 0.000 description 3
- 235000002722 Dioscorea batatas Nutrition 0.000 description 3
- 208000005189 Embolism Diseases 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- 208000001435 Thromboembolism Diseases 0.000 description 3
- 201000011510 cancer Diseases 0.000 description 3
- 230000002490 cerebral effect Effects 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 235000009508 confectionery Nutrition 0.000 description 3
- 239000000470 constituent Substances 0.000 description 3
- 238000010828 elution Methods 0.000 description 3
- 238000011156 evaluation Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000000835 fiber Substances 0.000 description 3
- 239000000499 gel Substances 0.000 description 3
- 230000001077 hypotensive effect Effects 0.000 description 3
- 230000007774 longterm Effects 0.000 description 3
- 210000004072 lung Anatomy 0.000 description 3
- 239000012567 medical material Substances 0.000 description 3
- 231100000252 nontoxic Toxicity 0.000 description 3
- 230000003000 nontoxic effect Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 208000024891 symptom Diseases 0.000 description 3
- 208000011580 syndromic disease Diseases 0.000 description 3
- 230000001225 therapeutic effect Effects 0.000 description 3
- 230000035922 thirst Effects 0.000 description 3
- 201000005665 thrombophilia Diseases 0.000 description 3
- XNRNNGPBEPRNAR-JQBLCGNGSA-N thromboxane B2 Chemical compound CCCCC[C@H](O)\C=C\[C@H]1OC(O)C[C@H](O)[C@@H]1C\C=C/CCCC(O)=O XNRNNGPBEPRNAR-JQBLCGNGSA-N 0.000 description 3
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 3
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 3
- 210000002700 urine Anatomy 0.000 description 3
- LRFVTYWOQMYALW-UHFFFAOYSA-N 9H-xanthine Chemical compound O=C1NC(=O)NC2=C1NC=N2 LRFVTYWOQMYALW-UHFFFAOYSA-N 0.000 description 2
- 206010003210 Arteriosclerosis Diseases 0.000 description 2
- 206010008190 Cerebrovascular accident Diseases 0.000 description 2
- 102000029816 Collagenase Human genes 0.000 description 2
- 108060005980 Collagenase Proteins 0.000 description 2
- 206010010904 Convulsion Diseases 0.000 description 2
- 206010011224 Cough Diseases 0.000 description 2
- 240000001811 Dioscorea oppositifolia Species 0.000 description 2
- 235000003416 Dioscorea oppositifolia Nutrition 0.000 description 2
- 102000008946 Fibrinogen Human genes 0.000 description 2
- 108010049003 Fibrinogen Proteins 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- 208000031226 Hyperlipidaemia Diseases 0.000 description 2
- UGQMRVRMYYASKQ-UHFFFAOYSA-N Hypoxanthine nucleoside Natural products OC1C(O)C(CO)OC1N1C(NC=NC2=O)=C2N=C1 UGQMRVRMYYASKQ-UHFFFAOYSA-N 0.000 description 2
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 2
- 208000008589 Obesity Diseases 0.000 description 2
- 244000061456 Solanum tuberosum Species 0.000 description 2
- 235000002595 Solanum tuberosum Nutrition 0.000 description 2
- 208000006011 Stroke Diseases 0.000 description 2
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 2
- 230000001154 acute effect Effects 0.000 description 2
- 230000032683 aging Effects 0.000 description 2
- YZXBAPSDXZZRGB-DOFZRALJSA-N arachidonic acid Chemical compound CCCCC\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O YZXBAPSDXZZRGB-DOFZRALJSA-N 0.000 description 2
- 208000011775 arteriosclerosis disease Diseases 0.000 description 2
- 208000006673 asthma Diseases 0.000 description 2
- 210000000748 cardiovascular system Anatomy 0.000 description 2
- 235000012000 cholesterol Nutrition 0.000 description 2
- 229960002424 collagenase Drugs 0.000 description 2
- 230000036461 convulsion Effects 0.000 description 2
- 208000029078 coronary artery disease Diseases 0.000 description 2
- 235000014113 dietary fatty acids Nutrition 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 230000000916 dilatatory effect Effects 0.000 description 2
- 230000000857 drug effect Effects 0.000 description 2
- 210000003725 endotheliocyte Anatomy 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 210000003743 erythrocyte Anatomy 0.000 description 2
- 229930195729 fatty acid Natural products 0.000 description 2
- 239000000194 fatty acid Substances 0.000 description 2
- 150000004665 fatty acids Chemical class 0.000 description 2
- 229940012952 fibrinogen Drugs 0.000 description 2
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 239000011777 magnesium Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000002398 materia medica Substances 0.000 description 2
- 230000002503 metabolic effect Effects 0.000 description 2
- 235000010755 mineral Nutrition 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- 231100000957 no side effect Toxicity 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 235000020824 obesity Nutrition 0.000 description 2
- 230000036581 peripheral resistance Effects 0.000 description 2
- 238000010791 quenching Methods 0.000 description 2
- 230000008439 repair process Effects 0.000 description 2
- 210000002345 respiratory system Anatomy 0.000 description 2
- 150000003384 small molecules Chemical class 0.000 description 2
- 230000035943 smell Effects 0.000 description 2
- 208000010110 spontaneous platelet aggregation Diseases 0.000 description 2
- 230000002110 toxicologic effect Effects 0.000 description 2
- 231100000041 toxicology testing Toxicity 0.000 description 2
- 230000002936 tranquilizing effect Effects 0.000 description 2
- 230000002792 vascular Effects 0.000 description 2
- 210000003556 vascular endothelial cell Anatomy 0.000 description 2
- MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 1
- OYHQOLUKZRVURQ-NTGFUMLPSA-N (9Z,12Z)-9,10,12,13-tetratritiooctadeca-9,12-dienoic acid Chemical compound C(CCCCCCC\C(=C(/C\C(=C(/CCCCC)\[3H])\[3H])\[3H])\[3H])(=O)O OYHQOLUKZRVURQ-NTGFUMLPSA-N 0.000 description 1
- WRIDQFICGBMAFQ-UHFFFAOYSA-N (E)-8-Octadecenoic acid Natural products CCCCCCCCCC=CCCCCCCC(O)=O WRIDQFICGBMAFQ-UHFFFAOYSA-N 0.000 description 1
- BFSVOASYOCHEOV-UHFFFAOYSA-N 2-diethylaminoethanol Chemical compound CCN(CC)CCO BFSVOASYOCHEOV-UHFFFAOYSA-N 0.000 description 1
- LQJBNNIYVWPHFW-UHFFFAOYSA-N 20:1omega9c fatty acid Natural products CCCCCCCCCCC=CCCCCCCCC(O)=O LQJBNNIYVWPHFW-UHFFFAOYSA-N 0.000 description 1
- QSBYPNXLFMSGKH-UHFFFAOYSA-N 9-Heptadecensaeure Natural products CCCCCCCC=CCCCCCCCC(O)=O QSBYPNXLFMSGKH-UHFFFAOYSA-N 0.000 description 1
- 101800000263 Acidic protein Proteins 0.000 description 1
- 206010002383 Angina Pectoris Diseases 0.000 description 1
- 208000006820 Arthralgia Diseases 0.000 description 1
- 201000001320 Atherosclerosis Diseases 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 102000016938 Catalase Human genes 0.000 description 1
- 108010053835 Catalase Proteins 0.000 description 1
- 208000017667 Chronic Disease Diseases 0.000 description 1
- 206010053567 Coagulopathies Diseases 0.000 description 1
- 206010069729 Collateral circulation Diseases 0.000 description 1
- 206010010071 Coma Diseases 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 201000006306 Cor pulmonale Diseases 0.000 description 1
- 206010051055 Deep vein thrombosis Diseases 0.000 description 1
- 244000096857 Dioscorea batatas Species 0.000 description 1
- 235000006536 Dioscorea esculenta Nutrition 0.000 description 1
- 208000000059 Dyspnea Diseases 0.000 description 1
- 206010013975 Dyspnoeas Diseases 0.000 description 1
- 102000010911 Enzyme Precursors Human genes 0.000 description 1
- 108010062466 Enzyme Precursors Proteins 0.000 description 1
- 208000007530 Essential hypertension Diseases 0.000 description 1
- 208000009386 Experimental Arthritis Diseases 0.000 description 1
- 208000022461 Glomerular disease Diseases 0.000 description 1
- 102000053187 Glucuronidase Human genes 0.000 description 1
- 108010060309 Glucuronidase Proteins 0.000 description 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 206010019468 Hemiplegia Diseases 0.000 description 1
- 108010033040 Histones Proteins 0.000 description 1
- 206010020608 Hypercoagulation Diseases 0.000 description 1
- 206010020741 Hyperpyrexia Diseases 0.000 description 1
- 208000001953 Hypotension Diseases 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 1
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 1
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 1
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
- 102000004882 Lipase Human genes 0.000 description 1
- 108090001060 Lipase Proteins 0.000 description 1
- 239000004367 Lipase Substances 0.000 description 1
- -1 Lumbrukinase Proteins 0.000 description 1
- 102000004317 Lyases Human genes 0.000 description 1
- 108090000856 Lyases Proteins 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- ZOKXTWBITQBERF-UHFFFAOYSA-N Molybdenum Chemical compound [Mo] ZOKXTWBITQBERF-UHFFFAOYSA-N 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- 102000008300 Mutant Proteins Human genes 0.000 description 1
- 108010021466 Mutant Proteins Proteins 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- 239000005642 Oleic acid Substances 0.000 description 1
- ZQPPMHVWECSIRJ-UHFFFAOYSA-N Oleic acid Natural products CCCCCCCCC=CCCCCCCCC(O)=O ZQPPMHVWECSIRJ-UHFFFAOYSA-N 0.000 description 1
- 206010030302 Oliguria Diseases 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 1
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 1
- 108091000080 Phosphotransferase Proteins 0.000 description 1
- 102000013566 Plasminogen Human genes 0.000 description 1
- 108010051456 Plasminogen Proteins 0.000 description 1
- 208000004880 Polyuria Diseases 0.000 description 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
- 208000004186 Pulmonary Heart Disease Diseases 0.000 description 1
- 102000018120 Recombinases Human genes 0.000 description 1
- 108010091086 Recombinases Proteins 0.000 description 1
- 208000001647 Renal Insufficiency Diseases 0.000 description 1
- 208000034189 Sclerosis Diseases 0.000 description 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 235000021355 Stearic acid Nutrition 0.000 description 1
- 208000005718 Stomach Neoplasms Diseases 0.000 description 1
- 208000007271 Substance Withdrawal Syndrome Diseases 0.000 description 1
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 1
- 208000024248 Vascular System injury Diseases 0.000 description 1
- 208000012339 Vascular injury Diseases 0.000 description 1
- 206010047249 Venous thrombosis Diseases 0.000 description 1
- 206010048222 Xerosis Diseases 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 231100000215 acute (single dose) toxicity testing Toxicity 0.000 description 1
- 238000011047 acute toxicity test Methods 0.000 description 1
- 238000009098 adjuvant therapy Methods 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 235000004279 alanine Nutrition 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 229940114079 arachidonic acid Drugs 0.000 description 1
- 235000021342 arachidonic acid Nutrition 0.000 description 1
- 230000006793 arrhythmia Effects 0.000 description 1
- 206010003119 arrhythmia Diseases 0.000 description 1
- 235000003704 aspartic acid Nutrition 0.000 description 1
- 206010003549 asthenia Diseases 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 230000004531 blood pressure lowering effect Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 230000008344 brain blood flow Effects 0.000 description 1
- 210000004958 brain cell Anatomy 0.000 description 1
- 210000005013 brain tissue Anatomy 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000000747 cardiac effect Effects 0.000 description 1
- 210000003169 central nervous system Anatomy 0.000 description 1
- 206010008118 cerebral infarction Diseases 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- XTEGARKTQYYJKE-UHFFFAOYSA-N chloric acid Chemical compound OCl(=O)=O XTEGARKTQYYJKE-UHFFFAOYSA-N 0.000 description 1
- 229940005991 chloric acid Drugs 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 230000004087 circulation Effects 0.000 description 1
- 230000035602 clotting Effects 0.000 description 1
- 230000015271 coagulation Effects 0.000 description 1
- 238000005345 coagulation Methods 0.000 description 1
- 239000010941 cobalt Substances 0.000 description 1
- 229910017052 cobalt Inorganic materials 0.000 description 1
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 210000003792 cranial nerve Anatomy 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000006735 deficit Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000001784 detoxification Methods 0.000 description 1
- 230000035487 diastolic blood pressure Effects 0.000 description 1
- 210000002249 digestive system Anatomy 0.000 description 1
- 230000010159 dioecy Effects 0.000 description 1
- 208000035475 disorder Diseases 0.000 description 1
- 230000035619 diuresis Effects 0.000 description 1
- 230000035622 drinking Effects 0.000 description 1
- 230000002124 endocrine Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 206010016256 fatigue Diseases 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 108010074605 gamma-Globulins Proteins 0.000 description 1
- 206010017758 gastric cancer Diseases 0.000 description 1
- 238000003304 gavage Methods 0.000 description 1
- 210000005086 glomerual capillary Anatomy 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- 230000003862 health status Effects 0.000 description 1
- 210000005003 heart tissue Anatomy 0.000 description 1
- 238000005534 hematocrit Methods 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- IPCSVZSSVZVIGE-UHFFFAOYSA-N hexadecanoic acid Chemical compound CCCCCCCCCCCCCCCC(O)=O IPCSVZSSVZVIGE-UHFFFAOYSA-N 0.000 description 1
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 201000001421 hyperglycemia Diseases 0.000 description 1
- 230000036543 hypotension Effects 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 229940125396 insulin Drugs 0.000 description 1
- QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 description 1
- 239000010977 jade Substances 0.000 description 1
- 201000006370 kidney failure Diseases 0.000 description 1
- 230000003907 kidney function Effects 0.000 description 1
- 230000002147 killing effect Effects 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 210000000088 lip Anatomy 0.000 description 1
- 235000019421 lipase Nutrition 0.000 description 1
- 230000037356 lipid metabolism Effects 0.000 description 1
- 210000003141 lower extremity Anatomy 0.000 description 1
- 235000018977 lysine Nutrition 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 206010062198 microangiopathy Diseases 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000011733 molybdenum Substances 0.000 description 1
- 229910052750 molybdenum Inorganic materials 0.000 description 1
- 208000010125 myocardial infarction Diseases 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 229910052759 nickel Inorganic materials 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 235000021049 nutrient content Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 description 1
- OQCDKBAXFALNLD-UHFFFAOYSA-N octadecanoic acid Natural products CCCCCCCC(C)CCCCCCCCC(O)=O OQCDKBAXFALNLD-UHFFFAOYSA-N 0.000 description 1
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 description 1
- 235000021313 oleic acid Nutrition 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000032696 parturition Effects 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 210000005259 peripheral blood Anatomy 0.000 description 1
- 239000011886 peripheral blood Substances 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
- 150000003904 phospholipids Chemical class 0.000 description 1
- 102000020233 phosphotransferase Human genes 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 230000010118 platelet activation Effects 0.000 description 1
- 150000004032 porphyrins Chemical class 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000011321 prophylaxis Methods 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 230000000171 quenching effect Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000002787 reinforcement Effects 0.000 description 1
- 230000008521 reorganization Effects 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 229910052711 selenium Inorganic materials 0.000 description 1
- 239000011669 selenium Substances 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 239000008117 stearic acid Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 201000011549 stomach cancer Diseases 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 229910052712 strontium Inorganic materials 0.000 description 1
- CIOAGBVUUVVLOB-UHFFFAOYSA-N strontium atom Chemical compound [Sr] CIOAGBVUUVVLOB-UHFFFAOYSA-N 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 230000035488 systolic blood pressure Effects 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 230000009424 thromboembolic effect Effects 0.000 description 1
- 230000001732 thrombotic effect Effects 0.000 description 1
- 231100000820 toxicity test Toxicity 0.000 description 1
- 238000009602 toxicology test Methods 0.000 description 1
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 229940035893 uracil Drugs 0.000 description 1
- 235000014393 valine Nutrition 0.000 description 1
- 239000004474 valine Substances 0.000 description 1
- 208000019553 vascular disease Diseases 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 239000000052 vinegar Substances 0.000 description 1
- 235000021419 vinegar Nutrition 0.000 description 1
- 238000013389 whole blood assay Methods 0.000 description 1
- 229940075420 xanthine Drugs 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses an earthworm protein for reducing blood sugar and blood pressure and eliminating diabetic complications. The earthworm protein is prepared by adopting the following steps of: 1) cleaning earthworms, namely, selecting live earthworms, removing impurities and dead earthworms, putting the live earthworms into water of which the pH value is 4 to 5.8 for 10 to 15 minutes, meanwhile, adding 50 g of salt, leaching the cleaned live earthworms, and flushing the live earthworms by using warm water of 28 DEG C to remove redundant moisture and impurities on the outer surfaces; 2) pulping; 3) filtering or centrifuging the homogenate obtained in the step 2) to remove rough residue therein, and filtering the residue removed homogenate to obtain clear liquor, wherein each milliliter of the clear liquor contains more than 180 enzyme activity units; putting the clear liquor into a disinfected container, injecting high-pressure CO2 gas, mechanically and fully stirring the clear liquor to be treated for 3 to 8 minutes, and releasing pressure through a conduit, wherein the solubility of CO2 is quickly reduced after the pressure is reduced; then filtering to remove impurities, and settling for 12 hours to obtain supernate; and 4) freeze-drying, namely drying the supernate in a freeze drier of 28 DEG C below zero to obtain a powdery product, wherein each milligram of the product contains 2,000 to 3,000 enzyme activity units.
Description
Technical field
The invention belongs to field of health care products, relate in particular to earthworm protein and the application thereof of a kind of blood sugar lowering, microcirculation improvement, elimination complication.
Background technology
Earthworm protein is extracted from Pheretima, contains multiple compositions such as collagenase, fibrinolysin, Lumbrukinase, nucleic acid, trace element, and its molecular weight belongs to the short chain small-molecule substance at 5000-10000.Pheretima extract (comprising earthworm protein etc.) has wide influence to the blood coagulation system in the body and fibrinolytic system, both can obviously reduce the rat platelet adherence rate, thrombosis and dissolving thrombus in vivo can increase the rat brain blood flow again in the extension body, reduce cerebral vascular resistance.Pheretima extract can also improve the mouse red blood cell deformability, thereby improves hemorheological property and microcirculation disturbance.
Nutritive value
Rich in proteins in the Pheretima body in stem body multiselect 56～66%, is the half as much again of Semen sojae atricolor, Oryza glutinosa six times.Contain multiple free amino acid in the earthworm protein, they are the topmost nutritional labelings of Pheretima.Its protein is made up of 18 seed amino acids, wherein the content with leucine and glutamic acid is the highest, every hectogram sample contains 22.161 and 6.227 grams respectively, and next has aspartic acid, valine, lysine, arginine, alanine, glycine, methionine, serine, Soviet Union's chloric acid, phenylalanine, proline, histidine and tyrosine etc.Eight kinds of free amino acids that comprised needed by human.Therefore, Lumbricus is a kind of full-valence animal egg Huang Yuan.The lipid of Pheretima partly contains the fatty acid of stearic acid, Palmic acid, highly unsaturated fatty acid, straight-chain odd number carbon and paniculate fatty acid, phospholipid, cholesterol etc.Highly unsaturated fatty acid such as oleic acid, linoleic acid, arachidonic acid, linolenic content are higher, have higher pharmacologically active, can prevent and treat atherosclerosis, are one of effective ingredient of Pheretima blood circulation promoting and blood stasis dispelling, control cardiovascular and cerebrovascular disease.Simultaneously, these unsaturated fatty acids also have certain Nutrition to human body.
Pheretima also contains nucleotide compositions such as more hypoxanthine, xanthine, guanine, uracil, can provide requisite raw material for human body rib acid metabolic.Wherein. hypoxanthine still is the Pheretima blood pressure lowering, the bent active relative branch of relievining asthma.Trouble has abundant fibrinolysin and catalase, peroxidase, dismutase, 8-D glucuronidase, alkali phosphatase in the Pheretima. lipase, porphyrin synzyme etc.These enzymes are to the human body beneficial.Contain abundant trace element such as strontium, selenium, magnesium, zinc, copper, molybdenum, nickel, cobalt etc. in the Pheretima, these elements all are to guarantee that health institute is requisite.
Collagenase is arranged in the earthworm protein, fibrinolysin, earthworm protein, nucleic acid, multiple composition such as trace element, its molecular weight is at 5000-10000, belong to the short chain small-molecule substance, can enter blood capillary smoothly, little bolt in the dissolving blood capillary, allow the blood capillary of sclerosis recover elasticity, thereby remove microvascular obstruction, the torsional deformation situation, directly dissolving forms human thrombus's main substance fibrin, thereby the direct thrombolytic effect that performance is powerful, dissolve fresh and old thrombosis, can improve the anticoagulant function of human body self, can activate the differentiation characteristic of vascular endothelial cell, get through passages through which vital energy circulates, promote blood capillary regeneration, set up collateral circulation, for the heart and brain tissues of focal zone is opened up new blood access, repair downright bad cranial nerve cell, cure hemiplegia, thereby reach the effect that continues thrombolytic, to the prevention of cardiovascular and cerebrovascular disease, the rehabilitation of apoplexy sequela is significant.Simultaneously help to eliminate the aging relevant with microcirculation disturbance and tumor again and take place, also improved the multiple physiological function of whole body and health status, improve immunity.
Thrombus
Earthworm protein fibre rich protein resolvase can activate the fibrinoclase unit in the thrombosis, makes it to be converted into to have active fibrinoclase, and then the fibrin in the thrombus, fresh thromboembolism.Fibrinoclase in this product is direct thrombus again, the thromboembolism of old.Thrombus does not influence the clotting mechanism in the body, the side effect that does not cause bleeding.
Vessel softening
Active contained fibrinoclase and other active matter mass-energy are dissolved fibrin net on the atherosis blood vessel wall, eliminate attached pathological products such as cholesterol speckle thereon, promote synthesizing of vascular endothelial cell DNA, promote the reparation of vascular damaged, blood vessel dilating, recover blood vessel elasticity, make unblocked blood flowing, change to a certain extent and stoped arteriosclerotic natural history.
It is sticking to reduce blood
After taking active earthworm protein, along with the dissolving of thrombosis, softening, the microcirculatory improvement of blood vessel, reduced blood viscosity, make unusual hemorheology index recover normal, it is normal that the nail fold microcirculation is detected by transferring to unusually, thereby eliminated the hidden danger that cordis and cerebral accident takes place.
The two-ways regulation blood pressure
Active earthworm protein is improved the circumference of cannon bone state by recovering blood elasticity, blood vessel dilating, thrombus, microcirculation improvement, reduces the blood flow Peripheral resistance, blood viscosity lowering and have stable hypotensive effect.After essential hypertension is taken, can make controlling of blood pressure normal back near normal level; The concurrent hypertension of severe hypertension disease or cardiovascular and cerebrovascular vessel, clothes can strengthen the hypotensive effect of other depressor after this product, reduce its dosage and malicious 1 side effect, and this product is by improving blood quality and Peripheral resistance has regulating action to hypotension simultaneously.
Blood sugar lowering
Because the optimum positive source comprehensive effect of active earthworm protein has improved the multiple function of human body, has improved the interior environment of body, has promoted the recovery of islet function, after clothes this product certain blood sugar reducing function is arranged.
Property of raw material
Earthworm protein is extracted from Pheretima, and Pheretima has another name called Lumbricus, and [3] Pheretima is one of important Chinese crude drug of China.In 67 kinds of animal drugs recording in the Chinese materia medica monograph Shennong's Herbal the earliest Lumbricus is just arranged.Classify low-grades as at Shennong's Herbal, have heat clearing away arresting convulsion, collateral dredging, relieving asthma, the effect of diuresis.Be used for diseases such as hyperpyrexia, coma, infantile convulsion tic, arthralgia, dyspnea and cough due to lung-heat, oliguria edema, hypertension after the normal parch.The main several amino acids that contains.Because the product of giving birth to fishy smell is too heavy, generally need through parch so be used as medicine.TAO Hong-Jing meaning: " if the dried earthworm of clothes must be endured and consider to be worth doing ".Tradition use Pheretima, and method for making is a lot, mainly contains medicine system, vinegar system, boils, methods such as processed with wine, oil system, Pulvis concha Mactrae parch, processed with salt, makes its quality loosely steep crisp, detoxification, correct stink and be convenient to fried taking.
Pheretima is except having better nutritivity value, and it also has a lot of health cares, can strengthen the premunition of human body, improves people's health level.In recent ten years, Pheretima has been carried out big quantity research chemical, the pharmacology aspect both at home and abroad, find that Pheretima not only contains rich nutrient contents, also contain multiple pharmacological component, have many-sided pharmacological action, relate to each system of human body such as cardiovascular system, blood system, immune system, central nervous system, respiratory system, digestive system etc.Especially unify aspect the blood system at cardiovascular system, Pheretima is prophylaxis of hypertension, myocardial infarction, arrhythmia, apoplexy thromboembolism well.
Application
The thrombus disease aspect
Earthworm protein has thrombolytic effect, and its effective ingredient is white enzyme of dawn.This kind of enzyme is can the direct hydrolysis fiber dawn white, and has the fight effect of living of inviting of proenzyme of fibre.Real fine horse result shows that the effective ingredient of this fibrinolysin thrombolytic is present in that DEAE ties up the 5th dawn white peak of fine plain column chromatography and the 2nd dawn white peak of sepbadeG-50 column chromatography sympathized.This dimension lyase energy anticoagulant, anticoagulant, reduction blood viscosity, thus thrombosis stoped.
The cardiovascular and cerebrovascular disease aspect
Experiment shows, earthworm protein can obviously increase blood flow rate and the blood flow of rat serum circulation, reduce hematocrit percentage rate and the blood viscosity of cell, obviously improve percentage composition and the erythrocyte Mg of blood total protein ++ content, explanatorily some chemical constituent of dragon protein really can be to some common cardiac and cerebral vascular diseases, as hypertension, coronary heart disease, arteriosclerosis and the fatigue disease of other blood etc., good prevention effect is arranged all.
Anti-tumor aspect
Find during with Pheretima extracting solution treatment gastric cancer that this extracting solution has significant inhibitory effect to cancerous cell DNA synthetic; After mice with tumor treatment 38 days, tumor cell complete obiteration as a result.Also have many about treating multiple cancer patient with earthworm protein and all obtaining the clinical report of certain curative effect over the years.This all explanatorily some contained chemical constituent of dragon protein the cancerous protuberance cell is had very strong inhibition differentiation and killing action.Or may coagulate the human blood hypercoagulability that the factor causes because of cancerous cell secretion cancer because some activation factor has been removed cancer patient, thereby the therapeutic purposes that play blood circulation promoting and blood stasis dispelling, remove swollen dispersing tumor.
Earthworm protein has been proved to be clinical value preferably, and it is a kind of polymolecular reorganization oral formulations, with thrombosis (fibrin) special affinity is arranged, can follow the tracks of thrombolytic, effectively dissolve little bolt, microcirculation improvement adds heart tonifying, cerebrovascular side Zhi Xunhuan, the open vascular injury endotheliocyte of repairing, increase blood vessel elasticity, improve blood vessel oxygen supply function, blood viscosity lowering, reduce platelet aggregation rate, suppress thrombosis and form again.Repair the downright bad brain cell of thrombosis generation rear perimeter edge, save half dark space.Be widely used in clinically at present, and be used in the heart, cerebrovascular, endocrine, prevention and treatment of diseases centers such as respiratory system more and more.
Present clinical trial shows that earthworm protein is definite to commonly encountered diseases therapeutical effect such as angina pectoris, cerebral infarction, diabetes, the nephrotic syndrome, pulmonary heart disease and deep vein thrombosis of lower extremity, and bad reflection is little, and the patient easily tolerates.Although there still have many problems to need to be extensive, clinical control is researched and solved for a long time, looks forward to the future, and the application of earthworm protein in clinical disease has better prospect.
At present, the application of China's Chinese medicine has more and more obtained the approval of international community, its multiple medical material is evident in efficacy, the medicine that is used for blood sugar lowering, blood pressure lowering, blood fat reducing is various especially, but real evident in efficacy in the practical application, have only several, for example Herb Gynostemmae Pentaphylli, Semen Cassiae etc., and the application mention pulse family Cassia sophera Cassia sophera L., use then seldom.The applicant finds in the production of reality with in using in the leaching process in earthworm protein, compound a certain amount of blood-sugar lowering tcm drug, the earthworm protein of locating of compound extraction has remarkable result aspect blood sugar lowering, significant curative effect is arranged in fat-reducing, blood fat reducing and blood pressure lowering too, especially after adding the batching of certain proportional quantity, effect is obviously strengthened.
Earthworm protein is called as the king of thrombolytic in medical circle, is extracted from Lumbricus.Be applied to rapid osmotic behind the affected part, thrombus becomes silted up and hinders, and makes unobstructed blood vessel, and varicosis falls after rise gradually.Aspects such as earthworm protein treatment diabetes, kidney syndrome have remarkable result equally.
Summary of the invention
For realizing purpose of the present invention, concrete scheme is as follows:
The earthworm protein of a kind of blood sugar lowering, microcirculation improvement, elimination complication is prepared by following steps:
1) Lumbricus is cleaned: select Lumbricus alive for use, remove impurity and dead rotten Lumbricus, it is 4～5.8 water 10～15 minutes that Lumbricus is put into pH value, adds 50g Sal simultaneously, and illumination simultaneously 2-3 hour impels Lumbricus to get rid of the interior dirt of body and cleans the Lumbricus body surface; Lumbricus alive after cleaning is carried out drip washing, use 28 ℃ warm water washing, wash away excessive moisture and the impurity of outer surface; 2) slurrying: be positioned in the container after the Lumbricus that step 1) is obtained is weighed, meter is put into 30～50 parts by weight, pH value is 10～20 parts of 5.0～5.3 sodium citrates, put into 10～30 parts of Chinese medicine composite powders again, described Chinese medicine composite powder is: 5 parts of Semen Vaccariae, 3 parts of the Radixs Astragali, 2 parts of Rhizoma Dioscoreaes, 2 parts in the Radix Rehmanniae, 2 parts of Fructus Schisandrae Chinensis, 1 part of Radix Ophiopogonis, 1 part of Fructus Mume, 1 part of Rhizoma Polygonati, 1.5 parts of Fructus Lyciis, 1 part of Radix Puerariae, 1 part on Folium Mori, 1 part of Cordyceps, 2 parts of Fructus Jujubaes, 2 parts of Arillus Longan, 2 parts of Cassia sophera Linn seeds are crushed to 350～500 orders through super micron mill and make powder and stir and be composited; The pure water that adds 3～8 times of Lumbricus weight then, with pearl mill, colloidal mill or high pressure homogenizer above-mentioned Lumbricus is carried out cell breakage after, add the sodium citrate of above-mentioned parts by weight and Chinese medicine composite powder and fully stir and make homogenate.3) with step 2) homogenate that obtains is by filtering or the centrifugal thick slag of removing wherein of sedimentation centrifuge, homogenate after the slagging-off, adopt per minute 6000 to leave centrifugal 16～24 minutes of scheming or the processing of 0.35 micron pore size membrane filtration, obtain clear liquor, described clear liquor be every milliliter greater than the liquid of 180 enzyme activity units; 4) saltout: add ammonium sulfate by 35% saturated concentration in the step 3) gained clear liquor, leave standstill after stirring is dissolved fully and got mixed liquor in 50 minutes; 5) centrifugal ultrafiltration: with the vertical and high-speed centrifuge through high speed centrifugation with step 4) in the mixed liquor thin slag remove, make clear liquid, by the 50000dt membrane ultrafiltration, get ultrafiltrate again.6) chromatographic column separates: adopt anion-exchange chromatography post or affinity column separating step 5) ultrafiltrate that obtains, with petroleum ether, 0.005～1.0 mole of arginine or sodium chloride solution eluting, collect the chromatography effluent of active peak part; 7) chromatography effluent sterilization: with above-mentioned steps 6) is put into the sealed sterilised container of carrying pipe, in container, inject high pressure CO 2 gases through conduit, machinery fully stirs pending clear liquor 5～8min simultaneously, through conduit output release pressure, pressure reduces back CO2 dissolubility and reduces rapidly, the rapid loss of a large amount of CO2 gases in the container; Filter removal of contamination then, precipitate 10 hours and get supernatant; 8) with the lyophilization of step 7) gained supernatant, in subzero 28～35 degree freezer dryers freezing 5～8 hours, under the vacuum of 0.2mmHg, be warming up to 25～30 ℃ then, dry 8 hours; Continue to be warming up to 35～39 ℃, vacuum drying 10～12 hours obtains powdery product, every milligram of 2000～3000 enzyme activity units.Described Abelmoschus esculentus is Abelmoschus esculentus stem and leaf, flower and the seed after cleaning up and drying.
Preferably, the earthworm protein of a kind of blood sugar lowering, microcirculation improvement, elimination complication, described Lumbricus is selected from: day stannum Dula earthworm, Japanese Dula earthworm, Pheretima aspergillum, Bing Shi Pheretimatschiliensis, Amythas dancatala, flat greatly No. two earthworms.
Preferably, the earthworm protein of a kind of blood sugar lowering, microcirculation improvement, elimination complication, described Lumbricus is selected from: day stannum Dula earthworm, Japanese Dula earthworm, Pheretima aspergillum, Bing Shi Pheretimatschiliensis, Amythas dancatala, flat greatly No. two earthworms; The described Lumbricus of selecting for use began to feed Cassia sophera Linn seed powder or Rhizoma Dioscoreae powder or Radix Et Rhizoma Fagopyri Tatarici powder or oatmeal or Abelmoschus esculentus powder in advance in 30 days, continue to feed the raw material that is elected to be the preparation earthworm protein after 30 days, described Cassia sophera Linn seed powder or Rhizoma Dioscoreae powder or Radix Et Rhizoma Fagopyri Tatarici powder or oatmeal or Abelmoschus esculentus powder are that Cassia sophera Linn seed, Rhizoma Dioscoreae, Radix Et Rhizoma Fagopyri Tatarici, Herba bromi japonici, Abelmoschus esculentus drying are crushed to the powder that 350～500 orders are made by super micron mill.
Preferably, the health care oral liquid that the earthworm protein of a kind of blood sugar lowering, microcirculation improvement, elimination complication is made, by weight, get 30～50 parts in the earthworm protein powder of above-mentioned blood sugar lowering, microcirculation improvement, elimination complication, adopt the superfine grinding apparatus processing to pulverize again; Make the powder grain diameter of pulverizing reach standby between 1～160 micron; Make again in every 100CC oral liquid for decreasing blood sugar of processing and contain 1-55g glucomannan powder, 3～15g xylosic alcohol powder; Its raw material sum is 100% ratio; After in above 72g raw material, adding the 90CC distilled water earlier again; Through abundant stirring and evenly mixing after-filtration, again distilled water is added to 100CC in the stock solution after filtration; Get the stock solution embedding at last, irradiation sterilization, after the check, packed products;
The health promoting wine that the earthworm protein of a kind of blood sugar lowering, blood fat reducing, blood pressure lowering, microcirculation improvement, elimination diabetic complication is made, it is characterized in that, the described Lumbricus of selecting for use began to feed Cassia sophera Linn seed powder or Rhizoma Dioscoreae powder or Radix Et Rhizoma Fagopyri Tatarici powder or oatmeal or Abelmoschus esculentus powder in advance in 30 days, continue to feed the raw material that is elected to be the preparation earthworm protein after 30 days, described Cassia sophera Linn seed powder or Rhizoma Dioscoreae powder or Radix Et Rhizoma Fagopyri Tatarici powder or oatmeal or Abelmoschus esculentus powder are that Cassia sophera Linn seed, Rhizoma Dioscoreae, Radix Et Rhizoma Fagopyri Tatarici, Herba bromi japonici, Abelmoschus esculentus drying are crushed to the powder that 350～500 orders are made by super micron mill; The Lumbricus of described nursing after full 30 days cleaned, and it is 4～5.8 water 10～15 minutes that Lumbricus is put into pH value, adds 50g Sal simultaneously, and illumination simultaneously 2-3 hour impels Lumbricus to get rid of in the body dirt and cleans the Lumbricus body surface; Lumbricus alive after cleaning is carried out drip washing, use 28 ℃ warm water washing, wash away the Lumbricus after the excessive moisture of outer surface and impurity obtain handling; Lumbricus after the above-mentioned processing adopts 55 degree cereal wines to soak or volume ratio is 1: 1 55 degree cereal wines and the mixed liquid dipping of 20 degree red wines, add Fructus Jujubae when soaking, Fructus Lycii, Arillus Longan, the supernatant that 11 days impurity screenings of the Cassia sophera Linn stem and leaf bubble of oven dry obtain, add described earthworm protein powder again and be described health promoting wine, wherein infusion proportioning is that 55 degree cereal wine 10kg or volume ratio are 1: 1 55 degree cereal wines and the mixed liquor 10kg of 20 degree red wines, Lumbricus 1kg after the described processing, Fructus Jujubae 300g, Fructus Lycii 1kg, Arillus Longan 100g, the Cassia sophera Linn stem and leaf 100g of oven dry, described earthworm protein powder 50g.
Preferably, the purposes of the earthworm protein of a kind of blood sugar lowering, microcirculation improvement, elimination complication, it is used to prepare the medicament for the treatment of or prevent diabetes.
Preferably, the purposes of the earthworm protein of a kind of blood sugar lowering, microcirculation improvement, elimination complication, it is used to prepare food or the health product for the treatment of or prevent diabetes.
Preferably, the purposes of the earthworm protein of a kind of blood sugar lowering, microcirculation improvement, elimination complication, it is used to prepare the medicament for the treatment of or the prevention nephrotic syndrome.
Described Chinese medicinal ingredients is through verifying, put down in writing and test gained for many years, though their effect is different, but choosing of each Chinese medicine is not arbitrarily to be, the applicant is according to the proportioning of for many years each composition of test gained, it is not the raw material effect simple superposition with each composition, because may have multiple interactivelies such as interpromoting relation in five elements, mutual reinforcement between, mutual restraint between two drugs or the mutual-detoxication between the various raw material of Chinese medicine, and the applicant has selected above-mentioned raw materials accordingly according to this principle exactly, and determined the best proportioning between them, therefore, the application has significant meaning.
Beneficial effect:
It is raw material sources that the present invention selects natural animal and plant medical material for use, utilize the comprehensive function of each natural constituents, treatment diabetic complication, kidney syndrome, through acute toxicology and long-term toxicological test, proof is to human body nonhazardous effect, its drug effect height can improve high blood coagulation state, improves blood fat, blood sugar lowering, thrombus, mediation microcirculation, improves immunity of organisms simultaneously.
The oral liquid that the earthworm protein that the present invention extracts is made, composition properties and characteristics be taupe, liquid, fragrant and sweet flavor is arranged, soluble in water.Show through the safety toxicology test: LD50 is 20000mg/kg, and the safety toxicological evaluation is nontoxic level, has no side effect.
Drink through human experiment, every day, 1-2 propped up, every 50ml.150 philtrums have obesity (with hyperlipidemia), hypertension, hyperglycemia symptom patient, and everyone drinks 30-60 days, and drug withdrawal 30-60 days.Obesity patient's (with hyperlipidemia symptom) 50 people wherein, through check fasting plasma lipid, T-CHOL CT, triglyceride TG and high density lipoprotein HDL, contrast test before and after drinking, carry out curative effect more relatively: test was drunk 30 days, experimenter TC, TG significantly descend, HDL obviously rises, and TC descends 32.2%, 43.3%, 55.5% respectively;
50 hypertension symptom persons wherein, drink 30 days after, obtain good blood pressure lowering effect simultaneously, mean blood pressure descends 30%, the blood pressure drops amplitude: systolic pressure decline 3.18kpa, diastolic pressure decline 2.62%kpa, its hypotensive effect is remarkable;
50 hyperglycemic patients are also obtained good blood sugar lowering, lipid-lowering effect; After the experimenter drinks 30 days, the TC 1.10mmol/L that on average descends, HDL rising 0.80mmol/L, the effect of TC and rising HDL wherein obviously descends, its effect is higher than vitamin E, the average rate of decrease that plays its glucose is 12.3%, and the average rate of decrease of fructosamine is that its blood glucose value of check all reaches normal level after 26.5%, 30 day.Result of the test shows: the health product effect that the present invention relates to is remarkable.
Show through pharmacological testing: it is raw material sources that the present invention selects natural animal and plant medical material for use, utilize the comprehensive function of each Chinese medicinal components, treatment diabetic syndrome and nephrotic syndrome, through acute toxicology and long-term toxicological test, proof is to human body nonhazardous effect, its drug effect height can improve high blood coagulation state, improves blood fat, blood sugar lowering, thrombus, mediation microcirculation, improves immunity of organisms simultaneously.
Specific embodiment
Embodiment 1
The Cassia sophera Linn Semen Cassiae, pulse family Cassia sophera Cassia sophera L..
Rhizoma Dioscoreae, the common name Rhizoma Dioscoreae.Herbaceos perennial, stem is overgrow, and often is with purple, and tuber is cylindrical, and leaf is to life, and is avette or oval, flower milky, dioecism.Starch-containing and the protein of tuber.Rhizoma Dioscoreae-medicinal part, plant name: Rhizoma Dioscoreae (y ù), the Latin language formal name used at school: Dioscorea opposita another name: Rhizoma Dioscoreae, triangle, RHIIZOMA DIOSCOREAE frOm Henan of China, Rhizoma dioscoreae, medicated egg, native potato, mountain potato, jade prolong.Abnormal smells from the patient: (root) is sweet, warm, flat, nontoxic.English name: nagaimo, yamaimo, Chinese yam, Japanese mountain yam, Korean yam; Compendium of Materia Medica " record, Rhizoma Dioscoreae is because the Tang Dynasty ancestor is Li Yu, for taboo changes Rhizoma Dioscoreae into, again because Song Yingzong is Zhao Shu, for taboo changes Rhizoma Dioscoreae into.Rhizoma Dioscoreae is one of composition medicine of LIUWEI DIHUANG WAN.[1] Rhizoma Dioscoreae " book on Chinese herbal medicine is looked for the truth ": " go in the drugs for nourishing yin and should give birth to usefulness, go into the suitable parching to brown of spleen reinforcing lung medicine and use." " this genus food, flat though gas is warm, be the moon of spleen reinforcing lung.Be moistening fur, longue meat, sweet in the mouth is held concurrently salty, again can the kidney tonifying reinforcing YIN-essence.", in the research of experimental arthritis rat, Rhizoma Dioscoreae water extract can significantly suppress the Denaturation of the gamma Globulin of Cu2+, shows to have antarthritic effect.Rhizoma Dioscoreae water extract also can be eliminated urine protein, mutant cell is had the effect that suppresses generation.Invigorating the spleen and replenishing QI: TIEGUISHANYAO is because being rich in kind of trace element surplus 18 seed amino acids and 10, and other mineral, so strengthening the spleen and stomach, tonifying the lung kidney, invigorating the spleen and replenishing QI, spleen invigorating tonify deficiency are arranged, the effects such as beneficial essence, beneficial heart and tranquilizing mind of reinforcing the kidney, saying of " all kinds of diseases and ailments are reinforced the kidney, controlled to spleen invigorating tonification, taste essence, treats five kinds of strain and seven kinds of impairment " arranged in the Li Shizhen (1518-1593 A.D.) Compendium of Material Medica.Quench one's thirst and promote the production of body fluid: this Rhizoma Dioscoreae has the effect of the life of quenching one's thirst: the Chinese traditional treatment asthenia is quenched one's thirst often has the Rhizoma Dioscoreae single to use in (diabetes) prescription, or share with other medicines, and effect is better.Health care: because fresh Rhizoma dioscoreae is rich in multivitamin, aminoacid and mineral, can prevent and treat the human body abnormalities of sugar/lipid metabolism, and arteriosclerosis, to safeguarding that the insulin normal function also has certain effect, the enhancing body immunity is arranged, beneficial heart and tranquilizing mind, rather cough health-care effects such as Dingchuan, slow down aging.Skin care: Yuan Dynasty taste expert Li Gao says: " control xerosis cutis and moisten it with this thing." Li Shizhen (1518-1593 A.D.) is write: " Rhizoma Dioscoreae can be moistened fur." Rhizoma Dioscoreae is to nourishes rough skin, and vigorous and graceful skin care has unique curative effect.
On effect, Cassia sophera Linn, Rhizoma Dioscoreae all are good at chronic diseases such as adjuvant therapy of diabetes complication.The earthworm protein product is more single in the market, does not have the precedent of earthworm protein and Chinese medicine Application of composite substantially, belongs to technological gap.The present invention utilizes the compound earthworm protein of Chinese medicine to make oral liquid through long-term test and clinical verification.Product meets the growing health care demand of people, future market promise well.Verified further that in clinical practice various medicines share by proportioning in the prescription, the single result of use of comparing obviously improves.
Other Chinese medicinal ingredients that relate to are through verifying, put down in writing and test gained for many years, though their effect is different, choosing of each Chinese medicine is not to be that length is limit, and does not carry out exhaustive at this arbitrarily;
The earthworm protein of a kind of blood sugar lowering, microcirculation improvement, elimination complication is prepared by following steps:
1) Lumbricus is cleaned: select Lumbricus alive for use, remove impurity and dead rotten Lumbricus, it is 4 water 10 minutes that Lumbricus is put into pH value, adds 50g Sal simultaneously, and illumination simultaneously 2 hours impels Lumbricus to get rid of the interior dirt of body and cleans the Lumbricus body surface; Lumbricus alive after cleaning is carried out drip washing, use 28 ℃ warm water washing, wash away excessive moisture and the impurity of outer surface; 2) slurrying: be positioned in the container after the Lumbricus that step 1) is obtained is weighed, meter is put into 30 parts by weight, pH value is 5.0 10 parts of sodium citrates, puts into 10 parts of Chinese medicine composite powders again, and described Chinese medicine composite powder is: 2 parts of 5 parts of Semen Vaccariae, 3 parts of the Radixs Astragali, 2 parts of Rhizoma Dioscoreaes, 2 parts in the Radix Rehmanniae, 2 parts of Fructus Schisandrae Chinensis, 1 part of Radix Ophiopogonis, 1 part of Fructus Mume, 1 part of Rhizoma Polygonati, 1.5 parts of Fructus Lyciis, 1 part of Radix Puerariae, 1 part on Folium Mori, 1 part of Cordyceps, 2 parts of Fructus Jujubaes, 2 parts of Arillus Longan, Cassia sophera Linn seed are crushed to 350～500 orders through super micron mill and make powder and stir and be composited; The pure water that adds 3 times of Lumbricus weight then, with pearl mill, colloidal mill or high pressure homogenizer above-mentioned Lumbricus is carried out cell breakage after, add the sodium citrate of above-mentioned parts by weight and Chinese medicine composite powder and fully stir and make homogenate.3) with step 2) homogenate that obtains is by filtering or the centrifugal thick slag of removing wherein of sedimentation centrifuge, homogenate after the slagging-off, adopt per minute 6000 to leave centrifugal 16 minutes of scheming or the processing of 0.35 micron pore size membrane filtration, obtain clear liquor, described clear liquor be every milliliter greater than the liquid of 180 enzyme activity units; 4) saltout: add ammonium sulfate by 35% saturated concentration in the step 3) gained clear liquor, leave standstill after stirring is dissolved fully and got mixed liquor in 50 minutes; 5) centrifugal ultrafiltration: with the vertical and high-speed centrifuge through high speed centrifugation with step 4) in the mixed liquor thin slag remove, make clear liquid, by the 50000dt membrane ultrafiltration, get ultrafiltrate again.6) chromatographic column separates: adopt anion-exchange chromatography post or affinity column separating step 5) ultrafiltrate that obtains, with petroleum ether, 0.005～1.0 mole of arginine or sodium chloride solution eluting, collect the chromatography effluent of active peak part; 7) chromatography effluent sterilization: with above-mentioned steps 6) is put into the sealed sterilised container of carrying pipe, injects high pressure CO through conduit in container
2Gas, machinery fully stirs pending clear liquor 5min simultaneously, and through conduit output release pressure, pressure reduces back CO
2Dissolubility reduces rapidly, a large amount of CO in the container
2The rapid loss of gas; Filter removal of contamination then, precipitate 10 hours and get supernatant; 8) with the lyophilization of step 7) gained supernatant, in subzero 28～35 degree freezer dryers freezing 5 hours, under the vacuum of 0.2mmHg, be warming up to 25 ℃ then, dry 8 hours; Continue to be warming up to 35 ℃, vacuum drying 10 hours obtains powdery product, every milligram of 2000～3000 enzyme activity units.
Wherein chromatographic column separates employing Mono-Q or Q-agarose gel H.P chromatography, carries out eluting with 0.005～1.0 mole of arginine.Perhaps adopt carboxymethyl-or DEAE-glucose gel or agarose gel column chromatography, go out the different earthworm protein of molecular weight with 1.0～1.5 mole nacls are gradient elution separation.
Preferably, the earthworm protein of a kind of blood sugar lowering, microcirculation improvement, elimination complication, described Lumbricus is selected from: day stannum Dula earthworm, Japanese Dula earthworm, Pheretima aspergillum, Bing Shi Pheretimatschiliensis, Amythas dancatala, flat greatly No. two earthworms.
Preferably, the earthworm protein of a kind of blood sugar lowering, microcirculation improvement, elimination complication, described Lumbricus is selected from: day stannum Dula earthworm, Japanese Dula earthworm, Pheretima aspergillum, Bing Shi Pheretimatschiliensis, Amythas dancatala, flat greatly No. two earthworms; The described Lumbricus of selecting for use began to feed Cassia sophera Linn seed powder or Rhizoma Dioscoreae powder or Radix Et Rhizoma Fagopyri Tatarici powder or oatmeal or Abelmoschus esculentus powder in advance in 30 days, continue to feed the raw material that is elected to be the preparation earthworm protein after 30 days, described Cassia sophera Linn seed powder or Rhizoma Dioscoreae powder or Radix Et Rhizoma Fagopyri Tatarici powder or oatmeal or Abelmoschus esculentus powder are that Cassia sophera Linn seed, Rhizoma Dioscoreae, Radix Et Rhizoma Fagopyri Tatarici, Herba bromi japonici, Abelmoschus esculentus drying are crushed to the powder that 350～500 orders are made by super micron mill.
Preferably, the health care oral liquid that the earthworm protein of a kind of blood sugar lowering, microcirculation improvement, elimination complication is made, by weight, get 30～50 parts in the earthworm protein powder of above-mentioned blood sugar lowering, microcirculation improvement, elimination complication, adopt the superfine grinding apparatus processing to pulverize again; Make the powder grain diameter of pulverizing reach standby between 1～160 micron; Make again in every 100CC oral liquid for decreasing blood sugar of processing and contain 1-55g glucomannan powder, 3～15g xylosic alcohol powder; Its raw material sum is 100% ratio; After in above 72g raw material, adding the 90CC distilled water earlier again; Through abundant stirring and evenly mixing after-filtration, again distilled water is added to 100CC in the stock solution after filtration; Get the stock solution embedding at last, irradiation sterilization, after the check, packed products;
The health promoting wine that the earthworm protein of a kind of blood sugar lowering, blood fat reducing, blood pressure lowering, microcirculation improvement, elimination diabetic complication is made, it is characterized in that, the described Lumbricus of selecting for use began to feed Cassia sophera Linn seed powder or Rhizoma Dioscoreae powder or Radix Et Rhizoma Fagopyri Tatarici powder or oatmeal or Abelmoschus esculentus powder in advance in 30 days, continue to feed the raw material that is elected to be the preparation earthworm protein after 30 days, described Cassia sophera Linn seed powder or Rhizoma Dioscoreae powder or Radix Et Rhizoma Fagopyri Tatarici powder or oatmeal or Abelmoschus esculentus powder are that Cassia sophera Linn seed, Rhizoma Dioscoreae, Radix Et Rhizoma Fagopyri Tatarici, Herba bromi japonici, Abelmoschus esculentus drying are crushed to the powder that 350～500 orders are made by super micron mill; The Lumbricus of described nursing after full 30 days cleaned, and it is 4～5.8 water 10～15 minutes that Lumbricus is put into pH value, adds 50g Sal simultaneously, and illumination simultaneously 2-3 hour impels Lumbricus to get rid of in the body dirt and cleans the Lumbricus body surface; Lumbricus alive after cleaning is carried out drip washing, use 28 ℃ warm water washing, wash away the Lumbricus after the excessive moisture of outer surface and impurity obtain handling; Lumbricus after the above-mentioned processing adopts 55 degree cereal wines to soak or volume ratio is 1: 1 55 degree cereal wines and the mixed liquid dipping of 20 degree red wines, add Fructus Jujubae when soaking, Fructus Lycii, Arillus Longan, the supernatant that 11 days impurity screenings of the Cassia sophera Linn stem and leaf bubble of oven dry obtain, add described earthworm protein powder again and be described health promoting wine, wherein infusion proportioning is that 55 degree cereal wine 10kg or volume ratio are 1: 1 55 degree cereal wines and the mixed liquor 10kg of 20 degree red wines, Lumbricus 1kg after the described processing, Fructus Jujubae 300g, Fructus Lycii 1kg, Arillus Longan 100g, the Cassia sophera Linn stem and leaf 100g of oven dry, described earthworm protein powder 50g.
Preferably, the purposes of the earthworm protein of a kind of blood sugar lowering, microcirculation improvement, elimination complication, it is used to prepare the medicament for the treatment of or prevent diabetes.
Preferably, the purposes of the earthworm protein of a kind of blood sugar lowering, microcirculation improvement, elimination complication, it is used to prepare food or the health product for the treatment of or prevent diabetes.
Preferably, the purposes of the earthworm protein of a kind of blood sugar lowering, microcirculation improvement, elimination complication, it is used to prepare the medicament for the treatment of or the prevention nephrotic syndrome.
Acute toxicity test:
Experimental subject: 10 of 20-23g healthy mices, male and female half and half
Experiment medicine: the oral liquid that earthworm protein dry powder of the present invention is made
Test method: 50 times, 100 times dosage according to the human body administration gavages to white mice, negative control group gives the normal saline of matched doses, animal is freely taken the photograph life and the death condition that the diseases caused by retention of fluid food is observed animal, administration after 10 days is slaughtered the white mice of survival, get the variation of every index of its its peripheral blood of whole blood assay, take to improve karber's method (The Fourth Military Medical University's journal, the 7th phase in 2002) and ask LD
50Be 20000mg/kg, the safety toxicological evaluation is nontoxic level, has no side effect.
Above-mentioned earthworm protein is made oral liquid, is that the patient with diabetes carries out pharmacological evaluation to 50.50 hyperglycemic patients are obtained good blood sugar lowering, lipid-lowering effect; After the experimenter drinks 30 days, the TC 1.05mmol/L that on average descends, HDL rising 0.85mmol/L, the effect of TC and rising HDL wherein obviously descends, its effect is higher than vitamin E, the average rate of decrease of glucose is 12.5%, and the average rate of decrease of fructosamine is that its blood glucose value of check all reaches normal level after 26.2%, 30 day.Result of the test shows: the health product effect that the present invention relates to is remarkable.
Earthworm protein treatment diabetes: blood plasma GMP140 is one of activation releasing product of platelet or endotheliocyte, studies show that in the blood plasma GMP140 Determination on content also can antimer in hematoblastic activation degree and thrombophilia, and TXB2 is the metabolic end product of XA2, and the latter has very strong short platelet aggregation and contracting blood vessel function.Many reports show that diabetics blood plasma GMP140 and TXB2 level significantly increase, and illustrate that platelet is in the state of activation in the diabetics body.The platelet adhesion aggregation capability increases, and this is one of reason of diabetics arteriosclerosis generation morning and incidence rate height and easy concurrent microangiopathies.Earthworm protein is that the compatriot from the artificial breeding likes to win the one group of acidic protein that extracts the Lumbricus.The oral earthworm protein 2 all backs blood plasma TXB2 levels of diabetics obviously descend, and do not have significant difference with matched group, and the prompting earthworm protein is assembled platelet adhesion the obvious suppression effect.Also significantly descend behind the blood plasma GMP140 horizontal stretcher, explanatorily dragon protein has the effect that suppresses the diabetics platelet activation to a certain extent.
The earthworm protein treatment nephrotic syndrome: earthworm protein is a histone hydrolytic enzyme that extracts from extraordinary Lumbricus.The preliminary study results of unit such as Tsing-Hua University showed that earthworm protein had thrombolytic in 1984, anticoagulant and go fine effect.Confirmed its mechanism of action at present, the one, activator of plasminogen (similar t-PA) effect is arranged; The 2nd, be combined with fibrin fibrin is degraded rapidly.Has direct and indirect thrombolytic function.The blood coagulation activity of coagulation system strengthens in the nephrotic syndrome patient Chang You.Fibrinogen increases and impels blood coagulation, impels microthrombusis in the glomerular capillary.NS patient's majority is in hypercoagulability, and thrombotic tendency is arranged.Results of study such as the attached Hou Ming of institute of Shandong medical university prove that fibrinolytic kinase activity (mainly being t-PA) reduces and fibrinolytic suppresses active (mainly being a2-PA) enhancing, are one of major reasons of the formation of chronic glomerulus disease high blood coagulation state and progress.Test check prompting fibrinogen and the blood viscosity index of this group significantly increase, and this conforms to substantially with more domestic scholars' report.Imagine this group and use the effect of earthworm protein treatment PNS, just be to replenish or increased the content of t-PA in the blood, thereby activate in the blood fibrinolytic system with the imbalance state that suppresses factor when making NS and be able to balance again, play anticoagulant and thrombolytic and improve the microcirculatory effect of glomerule, reach the purpose that reduces urine protein and improve renal function.The U.S. former permanent earth-worm extractive as raw material that uses of nineteen eighty-three Japan's Miyazaki University, the thousands of examples of clinical treatment thromboembolic disorders, effective percentage reaches more than 80%.Zhu waited report 9 routine primary glomerulopathies long the company in 1993, and the twenty-four-hour urine protein quantification obviously reduces before the treatment behind the oral earthworm protein extract, and 2 routine renal insufficiency persons are through having clear improvement after the earthworm protein treatment.Therapeutic effect is challenging.This organizes 20 routine PNS total effective rate after treating is 95%, and apparently higher than 65% of matched group, there is significant difference P＜0.05.Difference as for I type and II type curative effect may be long with its course of disease, and the degree of renal damage is heavier, and the histological type difference is relevant.Owing to multiple reason fails to do the research that concerns between pathology typing and the curative effect, remain further to be explored.
Embodiment 2
The earthworm protein of a kind of blood sugar lowering, microcirculation improvement, elimination complication is prepared by following steps:
1) Lumbricus is cleaned: select Lumbricus alive for use, remove impurity and dead rotten Lumbricus, it is 4.9 water 12.5 minutes that Lumbricus is put into pH value, adds 50g Sal simultaneously, and illumination simultaneously 2.5 hours impels Lumbricus to get rid of the interior dirt of body and cleans the Lumbricus body surface; Lumbricus alive after cleaning is carried out drip washing, use 28 ℃ warm water washing, wash away excessive moisture and the impurity of outer surface; 2) slurrying: be positioned in the container after the Lumbricus that step 1) is obtained is weighed, meter is put into 40 parts by weight, pH value is 5.1 15 parts of sodium citrates, puts into 15 parts of Chinese medicine composite powders again, and described Chinese medicine composite powder is: 2 parts of 5 parts of Semen Vaccariae, 3 parts of the Radixs Astragali, 2 parts of Rhizoma Dioscoreaes, 2 parts in the Radix Rehmanniae, 2 parts of Fructus Schisandrae Chinensis, 1 part of Radix Ophiopogonis, 1 part of Fructus Mume, 1 part of Rhizoma Polygonati, 1.5 parts of Fructus Lyciis, 1 part of Radix Puerariae, 1 part on Folium Mori, 1 part of Cordyceps, 2 parts of Fructus Jujubaes, 2 parts of Arillus Longan, Cassia sophera Linn seed are crushed to 400 orders through super micron mill and make powder and stir and be composited; The pure water that adds 5 times of Lumbricus weight then, with pearl mill, colloidal mill or high pressure homogenizer above-mentioned Lumbricus is carried out cell breakage after, add the sodium citrate of above-mentioned parts by weight and Chinese medicine composite powder and fully stir and make homogenate.3) with step 2) homogenate that obtains is by filtering or the centrifugal thick slag of removing wherein of sedimentation centrifuge, homogenate after the slagging-off, adopt per minute 6000 to leave centrifugal 20 minutes of scheming or the processing of 0.35 micron pore size membrane filtration, obtain clear liquor, described clear liquor be every milliliter greater than the liquid of 180 enzyme activity units; 4) saltout: add ammonium sulfate by 35% saturated concentration in the step 3) gained clear liquor, leave standstill after stirring is dissolved fully and got mixed liquor in 50 minutes; 5) centrifugal ultrafiltration: with the vertical and high-speed centrifuge through high speed centrifugation with step 4) in the mixed liquor thin slag remove, make clear liquid, by the 50000dt membrane ultrafiltration, get ultrafiltrate again.6) chromatographic column separates: adopt anion-exchange chromatography post or affinity column separating step 5) ultrafiltrate that obtains, with petroleum ether, 0.005～1.0 mole of arginine or sodium chloride solution eluting, collect the chromatography effluent of active peak part; 7) chromatography effluent sterilization: with above-mentioned steps 6) is put into the sealed sterilised container of carrying pipe, injects high pressure CO through conduit in container
2Gas, machinery fully stirs pending clear liquor 5～8min simultaneously, and through conduit output release pressure, pressure reduces back CO2 dissolubility and reduces rapidly, a large amount of CO in the container
2The rapid loss of gas; Filter removal of contamination then, precipitate 10 hours and get supernatant; 8) with the lyophilization of step 7) gained supernatant, in subzero 28～35 degree freezer dryers freezing 5～8 hours, under the vacuum of 0.2mmHg, be warming up to 25～30 ℃ then, dry 8 hours; Continue to be warming up to 35～39 ℃, vacuum drying 10～12 hours obtains powdery product, every milligram of 2000～3000 enzyme activity units.
Wherein chromatographic column separates employing Mono-Q or Q-agarose gel H.P chromatography, carries out eluting with 0.005～1.0 mole of arginine.Perhaps adopt carboxymethyl-or DEAE-glucose gel or agarose gel column chromatography, go out the different earthworm protein of molecular weight with 1.0～1.5 mole nacls are gradient elution separation.
Preferably, the earthworm protein of a kind of blood sugar lowering, microcirculation improvement, elimination complication, described Lumbricus is selected from: day stannum Dula earthworm, Japanese Dula earthworm, Pheretima aspergillum, Bing Shi Pheretimatschiliensis, Amythas dancatala, flat greatly No. two earthworms.
Preferably, the earthworm protein of a kind of blood sugar lowering, microcirculation improvement, elimination complication, described Lumbricus is selected from: day stannum Dula earthworm, Japanese Dula earthworm, Pheretima aspergillum, Bing Shi Pheretimatschiliensis, Amythas dancatala, flat greatly No. two earthworms; The described Lumbricus of selecting for use began to feed Cassia sophera Linn seed powder or Rhizoma Dioscoreae powder or Radix Et Rhizoma Fagopyri Tatarici powder or oatmeal or Abelmoschus esculentus powder in advance in 30 days, continue to feed the raw material that is elected to be the preparation earthworm protein after 30 days, described Cassia sophera Linn seed powder or Rhizoma Dioscoreae powder or Radix Et Rhizoma Fagopyri Tatarici powder or oatmeal or Abelmoschus esculentus powder are that Cassia sophera Linn seed, Rhizoma Dioscoreae, Radix Et Rhizoma Fagopyri Tatarici, Herba bromi japonici, Abelmoschus esculentus drying are crushed to the powder that 350～500 orders are made by super micron mill.
Preferably, the health care oral liquid that the earthworm protein of a kind of blood sugar lowering, microcirculation improvement, elimination complication is made, by weight, get 30～50 parts in the earthworm protein powder of above-mentioned blood sugar lowering, microcirculation improvement, elimination complication, adopt the superfine grinding apparatus processing to pulverize again; Make the powder grain diameter of pulverizing reach standby between 1～160 micron; Make again in every 100CC oral liquid for decreasing blood sugar of processing and contain 1-55g glucomannan powder, 3-15g xylosic alcohol powder; Its raw material sum is 100% ratio; After in above 72g raw material, adding the 90CC distilled water earlier again; Through abundant stirring and evenly mixing after-filtration, again distilled water is added to 100CC in the stock solution after filtration; Get the stock solution embedding at last, irradiation sterilization, after the check, packed products;
The health promoting wine that the earthworm protein of a kind of blood sugar lowering, blood fat reducing, blood pressure lowering, microcirculation improvement, elimination diabetic complication is made, it is characterized in that, the described Lumbricus of selecting for use began to feed Cassia sophera Linn seed powder or Rhizoma Dioscoreae powder or Radix Et Rhizoma Fagopyri Tatarici powder or oatmeal or Abelmoschus esculentus powder in advance in 30 days, continue to feed the raw material that is elected to be the preparation earthworm protein after 30 days, described Cassia sophera Linn seed powder or Rhizoma Dioscoreae powder or Radix Et Rhizoma Fagopyri Tatarici powder or oatmeal or Abelmoschus esculentus powder are that Cassia sophera Linn seed, Rhizoma Dioscoreae, Radix Et Rhizoma Fagopyri Tatarici, Herba bromi japonici, Abelmoschus esculentus drying are crushed to the powder that 350～500 orders are made by super micron mill; The Lumbricus of described nursing after full 30 days cleaned, and it is 4～5.8 water 10～15 minutes that Lumbricus is put into pH value, adds 50g Sal simultaneously, and illumination simultaneously 2-3 hour impels Lumbricus to get rid of in the body dirt and cleans the Lumbricus body surface; Lumbricus alive after cleaning is carried out drip washing, use 28 ℃ warm water washing, wash away the Lumbricus after the excessive moisture of outer surface and impurity obtain handling; Lumbricus after the above-mentioned processing adopts 55 degree cereal wines to soak or volume ratio is 1: 1 55 degree cereal wines and the mixed liquid dipping of 20 degree red wines, add Fructus Jujubae when soaking, Fructus Lycii, Arillus Longan, the supernatant that 11 days impurity screenings of the Cassia sophera Linn stem and leaf bubble of oven dry obtain, add described earthworm protein powder again and be described health promoting wine, wherein infusion proportioning is that 55 degree cereal wine 10kg or volume ratio are 1: 1 55 degree cereal wines and the mixed liquor 10kg of 20 degree red wines, Lumbricus 1kg after the described processing, Fructus Jujubae 300g, Fructus Lycii 1kg, Arillus Longan 100g, the Cassia sophera Linn stem and leaf 100g of oven dry, described earthworm protein powder 50g.
Preferably, the purposes of the earthworm protein of a kind of blood sugar lowering, microcirculation improvement, elimination complication, it is used to prepare the medicament for the treatment of or prevent diabetes.
Preferably, the purposes of the earthworm protein of a kind of blood sugar lowering, microcirculation improvement, elimination complication, it is used to prepare food or the health product for the treatment of or prevent diabetes.
Preferably, the purposes of the earthworm protein of a kind of blood sugar lowering, microcirculation improvement, elimination complication, it is used to prepare the medicament for the treatment of or the prevention nephrotic syndrome.
Above-mentioned earthworm protein is made oral liquid, is that the patient with diabetes carries out pharmacological evaluation to 50.50 hyperglycemic patients are obtained good blood sugar lowering, lipid-lowering effect; After the experimenter drinks 30 days, the TC 1.06mmol/L that on average descends, HDL rising 0.80mmol/L, the effect of TC and rising HDL wherein obviously descends, its effect is higher than vitamin E, the average rate of decrease that plays its glucose is 12.3%, and the average rate of decrease of fructosamine is that its blood glucose value of check all reaches normal level after 26.4%, 30 day.Result of the test shows: the health product effect that the present invention relates to is remarkable.
Embodiment 3
The earthworm protein of a kind of blood sugar lowering, microcirculation improvement, elimination complication is prepared by following steps:
1) Lumbricus is cleaned: select Lumbricus alive for use, remove impurity and dead rotten Lumbricus, it is 5.8 water 15 minutes that Lumbricus is put into pH value, adds 50g Sal simultaneously, and illumination simultaneously 3 hours impels Lumbricus to get rid of the interior dirt of body and cleans the Lumbricus body surface; Lumbricus alive after cleaning is carried out drip washing, use 28 ℃ warm water washing, wash away excessive moisture and the impurity of outer surface; 2) slurrying: be positioned in the container after the Lumbricus that step 1) is obtained is weighed, meter is put into 50 parts by weight, pH value is 5.3 20 parts of sodium citrates, puts into 30 parts of Chinese medicine composite powders again, and described Chinese medicine composite powder is: 2 parts of 5 parts of Semen Vaccariae, 3 parts of the Radixs Astragali, 2 parts of Rhizoma Dioscoreaes, 2 parts in the Radix Rehmanniae, 2 parts of Fructus Schisandrae Chinensis, 1 part of Radix Ophiopogonis, 1 part of Fructus Mume, 1 part of Rhizoma Polygonati, 1.5 parts of Fructus Lyciis, 1 part of Radix Puerariae, 1 part on Folium Mori, 1 part of Cordyceps, 2 parts of Fructus Jujubaes, 2 parts of Arillus Longan, Cassia sophera Linn seed are crushed to 500 orders through super micron mill and make powder and stir and be composited; The pure water that adds 8 times of Lumbricus weight then, with pearl mill, colloidal mill or high pressure homogenizer above-mentioned Lumbricus is carried out cell breakage after, add the sodium citrate of above-mentioned parts by weight and Chinese medicine composite powder and fully stir and make homogenate.3) with step 2) homogenate that obtains is by filtering or the centrifugal thick slag of removing wherein of sedimentation centrifuge, homogenate after the slagging-off, adopt per minute 6000 to leave centrifugal 24 minutes of scheming or the processing of 0.35 micron pore size membrane filtration, obtain clear liquor, described clear liquor be every milliliter greater than the liquid of 180 enzyme activity units; 4) saltout: add ammonium sulfate by 35% saturated concentration in the step 3) gained clear liquor, leave standstill after stirring is dissolved fully and got mixed liquor in 50 minutes; 5) centrifugal ultrafiltration: with the vertical and high-speed centrifuge through high speed centrifugation with step 4) in the mixed liquor thin slag remove, make clear liquid, by the 50000dt membrane ultrafiltration, get ultrafiltrate again.6) chromatographic column separates: adopt anion-exchange chromatography post or affinity column separating step 5) ultrafiltrate that obtains, with petroleum ether, 0.005～1.0 mole of arginine or sodium chloride solution eluting, collect the chromatography effluent of active peak part; 7) chromatography effluent sterilization: with above-mentioned steps 6) is put into the sealed sterilised container of carrying pipe, injects high pressure CO through conduit in container
2Gas, machinery fully stirs pending clear liquor 5～8min simultaneously, and through conduit output release pressure, pressure reduces back CO
2Dissolubility reduces rapidly, the rapid loss of a large amount of CO2 gases in the container; Filter removal of contamination then, precipitate 10 hours and get supernatant; 8) with the lyophilization of step 7) gained supernatant, in subzero 28～35 degree freezer dryers freezing 5～8 hours, under the vacuum of 0.2mmHg, be warming up to 25～30 ℃ then, dry 8 hours; Continue to be warming up to 35～39 ℃, vacuum drying 10～12 hours obtains powdery product, every milligram of 2000～3000 enzyme activity units.
Wherein chromatographic column separates employing Mono-Q or Q-agarose gel H.P chromatography, carries out eluting with 0.005～1.0 mole of arginine.Perhaps adopt carboxymethyl-or DEAE-glucose gel or agarose gel column chromatography, go out the different earthworm protein of molecular weight with 1.0～1.5 mole nacls are gradient elution separation.
Preferably, the earthworm protein of a kind of blood sugar lowering, microcirculation improvement, elimination complication, described Lumbricus is selected from: day stannum Dula earthworm, Japanese Dula earthworm, Pheretima aspergillum, Bing Shi Pheretimatschiliensis, Amythas dancatala, flat greatly No. two earthworms.
Preferably, the earthworm protein of a kind of blood sugar lowering, microcirculation improvement, elimination complication, described Lumbricus is selected from: day stannum Dula earthworm, Japanese Dula earthworm, Pheretima aspergillum, Bing Shi Pheretimatschiliensis, Amythas dancatala, flat greatly No. two earthworms; The described Lumbricus of selecting for use began to feed Cassia sophera Linn seed powder or Rhizoma Dioscoreae powder or Radix Et Rhizoma Fagopyri Tatarici powder or oatmeal or Abelmoschus esculentus powder in advance in 30 days, continue to feed the raw material that is elected to be the preparation earthworm protein after 30 days, described Cassia sophera Linn seed powder or Rhizoma Dioscoreae powder or Radix Et Rhizoma Fagopyri Tatarici powder or oatmeal or Abelmoschus esculentus powder are that Cassia sophera Linn seed, Rhizoma Dioscoreae, Radix Et Rhizoma Fagopyri Tatarici, Herba bromi japonici, Abelmoschus esculentus drying are crushed to the powder that 350～500 orders are made by super micron mill.
Preferably, the health care oral liquid that the earthworm protein of a kind of blood sugar lowering, microcirculation improvement, elimination complication is made, by weight, get 30～50 parts in the earthworm protein powder of above-mentioned blood sugar lowering, microcirculation improvement, elimination complication, adopt the superfine grinding apparatus processing to pulverize again; Make the powder grain diameter of pulverizing reach standby between 1～160 micron; Make again in every 100CC oral liquid for decreasing blood sugar of processing and contain 1-55g glucomannan powder, 3～15g xylosic alcohol powder; Its raw material sum is 100% ratio; After in above 72g raw material, adding the 90CC distilled water earlier again; Through abundant stirring and evenly mixing after-filtration, again distilled water is added to 100CC in the stock solution after filtration; Get the stock solution embedding at last, irradiation sterilization, after the check, packed products;
The health promoting wine that the earthworm protein of a kind of blood sugar lowering, blood fat reducing, blood pressure lowering, microcirculation improvement, elimination diabetic complication is made, it is characterized in that, the described Lumbricus of selecting for use began to feed Cassia sophera Linn seed powder or Rhizoma Dioscoreae powder or Radix Et Rhizoma Fagopyri Tatarici powder or oatmeal or Abelmoschus esculentus powder in advance in 30 days, continue to feed the raw material that is elected to be the preparation earthworm protein after 30 days, described Cassia sophera Linn seed powder or Rhizoma Dioscoreae powder or Radix Et Rhizoma Fagopyri Tatarici powder or oatmeal or Abelmoschus esculentus powder are that Cassia sophera Linn seed, Rhizoma Dioscoreae, Radix Et Rhizoma Fagopyri Tatarici, Herba bromi japonici, Abelmoschus esculentus drying are crushed to the powder that 350～500 orders are made by super micron mill; The Lumbricus of described nursing after full 30 days cleaned, and it is 4～5.8 water 10～15 minutes that Lumbricus is put into pH value, adds 50g Sal simultaneously, and illumination simultaneously 2-3 hour impels Lumbricus to get rid of in the body dirt and cleans the Lumbricus body surface; Lumbricus alive after cleaning is carried out drip washing, use 28 ℃ warm water washing, wash away the Lumbricus after the excessive moisture of outer surface and impurity obtain handling; Lumbricus after the above-mentioned processing adopts 55 degree cereal wines to soak or volume ratio is 1: 1 55 degree cereal wines and the mixed liquid dipping of 20 degree red wines, add Fructus Jujubae when soaking, Fructus Lycii, Arillus Longan, the supernatant that 11 days impurity screenings of the Cassia sophera Linn stem and leaf bubble of oven dry obtain, add described earthworm protein powder again and be described health promoting wine, wherein infusion proportioning is that 55 degree cereal wine 10kg or volume ratio are 1: 1 55 degree cereal wines and the mixed liquor 10kg of 20 degree red wines, Lumbricus 1kg after the described processing, Fructus Jujubae 300g, Fructus Lycii 1kg, Arillus Longan 100g, the Cassia sophera Linn stem and leaf 100g of oven dry, described earthworm protein powder 50g.
Preferably, the purposes of the earthworm protein of a kind of blood sugar lowering, microcirculation improvement, elimination complication, it is used to prepare the medicament for the treatment of or prevent diabetes.
Preferably, the purposes of the earthworm protein of a kind of blood sugar lowering, microcirculation improvement, elimination complication, it is used to prepare food or the health product for the treatment of or prevent diabetes.
Preferably, the purposes of the earthworm protein of a kind of blood sugar lowering, microcirculation improvement, elimination complication, it is used to prepare the medicament for the treatment of or the prevention nephrotic syndrome.
Above-mentioned earthworm protein is made oral liquid, is that the patient with diabetes carries out pharmacological evaluation to 50.50 hyperglycemic patients are obtained good blood sugar lowering, lipid-lowering effect; After the experimenter drinks 30 days, the TC 1.06mmol/L that on average descends, HDL rising 0.80mmol/L, the effect of TC and rising HDL wherein obviously descends, its effect is higher than vitamin E, the average rate of decrease that plays its glucose is 12.3%, and the average rate of decrease of fructosamine is that its blood glucose value of check all reaches normal level after 26.4%, 30 day.Result of the test shows: the health product effect that the present invention relates to is remarkable.
This explanation be that the numerical range that relates among the embodiment all is through repeatedly testing gained, each point can be realized, is not confined to end points or terminal point, length is limit, and does not enumerate one by one at this.
It should be noted that at last: above embodiment only in order to technical scheme of the present invention to be described, is not intended to limit; Although with reference to previous embodiment the present invention is had been described in detail, those of ordinary skill in the art is to be understood that: it still can be made amendment to the technical scheme that aforementioned each embodiment puts down in writing, and perhaps part technical characterictic wherein is equal to replacement; And these modifications or replacement do not make the essence of appropriate technical solution break away from the spirit and scope of various embodiments of the present invention technical scheme.
Claims (8)
1. the earthworm protein of a blood sugar lowering, blood fat reducing, blood pressure lowering, microcirculation improvement, elimination diabetic complication is characterized in that being prepared by following steps:
1) Lumbricus is cleaned: select Lumbricus alive for use, remove impurity and dead rotten Lumbricus, it is 4～5.8 water 10～15 minutes that Lumbricus is put into pH value, adds 50g Sal simultaneously, and illumination simultaneously 2-3 hour impels Lumbricus to get rid of the interior dirt of body and cleans the Lumbricus body surface; Lumbricus alive after cleaning is carried out drip washing, use 28 ℃ warm water washing, wash away excessive moisture and the impurity of outer surface; 2) slurrying: be positioned in the container after the Lumbricus that step 1) is obtained is weighed, meter is put into 30～50 parts by weight, pH value is 10～20 parts of 5.0～5.3 sodium citrates, put into 10～30 parts of Chinese medicine composite powders again, described Chinese medicine composite powder is: 5 parts of Semen Vaccariae, 3 parts of the Radixs Astragali, 2 parts of Rhizoma Dioscoreaes, 2 parts in the Radix Rehmanniae, 2 parts of Fructus Schisandrae Chinensis, 1 part of Radix Ophiopogonis, 1 part of Fructus Mume, 1 part of Rhizoma Polygonati, 1.5 parts of Fructus Lyciis, 1 part of Radix Puerariae, 1 part on Folium Mori, 1 part of Cordyceps, 2 parts of Fructus Jujubaes, 2 parts of Arillus Longan, 2 parts of Cassia sophera Linn seeds, being crushed to 350～500 orders through super micron mill makes powder and stirs and be composited; The pure water that adds 3～8 times of Lumbricus weight then, with pearl mill, colloidal mill or high pressure homogenizer above-mentioned Lumbricus is carried out cell breakage after, add the sodium citrate of above-mentioned parts by weight and Chinese medicine composite powder and fully stir and make homogenate.3) with step 2) homogenate that obtains is by filtering or the centrifugal thick slag of removing wherein of sedimentation centrifuge, homogenate after the slagging-off, adopt per minute 6000 to leave centrifugal 16～24 minutes of scheming or the processing of 0.35 micron pore size membrane filtration, obtain clear liquor, described clear liquor be every milliliter greater than the liquid of 180 enzyme activity units; 4) saltout: add ammonium sulfate by 35% saturated concentration in the step 3) gained clear liquor, leave standstill after stirring is dissolved fully and got mixed liquor in 50 minutes; 5) centrifugal ultrafiltration: with the vertical and high-speed centrifuge through high speed centrifugation with step 4) in the mixed liquor thin slag remove, make clear liquid, by the 50000dt membrane ultrafiltration, get ultrafiltrate again.6) chromatographic column separates: adopt anion-exchange chromatography post or affinity column separating step 5) ultrafiltrate that obtains, with petroleum ether, 0.005～1.0 mole of arginine or sodium chloride solution eluting, collect the chromatography effluent of active peak part; 7) chromatography effluent sterilization: with above-mentioned steps 6) is put into the sealed sterilised container of carrying pipe, injects high pressure CO through conduit in container
2Gas, machinery fully stirs pending clear liquor 5～8min simultaneously, and through conduit output release pressure, pressure reduces back CO
2Dissolubility reduces rapidly, a large amount of CO in the container
2The rapid loss of gas; Filter removal of contamination then, precipitate 10 hours and get supernatant; 8) with the lyophilization of step 7) gained supernatant, in subzero 28～35 degree freezer dryers freezing 5～8 hours, under the vacuum of 0.2mmHg, be warming up to 25～30 ℃ then, dry 8 hours; Continue to be warming up to 35～39 ℃, vacuum drying 10～12 hours obtains powdery product, every milligram of 2000～3000 enzyme activity units.
2. the earthworm protein of a kind of blood sugar lowering according to claim 1, blood fat reducing, blood pressure lowering, microcirculation improvement, elimination diabetic complication, it is characterized in that described Lumbricus is selected from: day stannum Dula earthworm, Japanese Dula earthworm, Pheretima aspergillum, Bing Shi Pheretimatschiliensis, Amythas dancatala, flat greatly No. two earthworms.
3. the earthworm protein of a kind of blood sugar lowering according to claim 2, blood fat reducing, blood pressure lowering, microcirculation improvement, elimination diabetic complication, it is characterized in that described Lumbricus is selected from: day stannum Dula earthworm, Japanese Dula earthworm, Pheretima aspergillum, Bing Shi Pheretimatschiliensis, Amythas dancatala, flat greatly No. two earthworms; The described Lumbricus of selecting for use began to feed Cassia sophera Linn seed powder or Rhizoma Dioscoreae powder or Radix Et Rhizoma Fagopyri Tatarici powder or oatmeal or Abelmoschus esculentus powder in advance in 30 days, continue to feed the raw material that is elected to be the preparation earthworm protein after 30 days, described Cassia sophera Linn seed powder or Rhizoma Dioscoreae powder or Radix Et Rhizoma Fagopyri Tatarici powder or oatmeal or Abelmoschus esculentus powder are that Cassia sophera Linn seed, Rhizoma Dioscoreae, Radix Et Rhizoma Fagopyri Tatarici, Herba bromi japonici, Abelmoschus esculentus drying are crushed to the powder that 350～500 orders are made by super micron mill.
4. the health care oral liquid made of the earthworm protein of the described a kind of blood sugar lowering of claim 1-3, blood fat reducing, blood pressure lowering, microcirculation improvement, elimination diabetic complication, it is characterized in that, by weight, get 30～50 parts in the earthworm protein powder of above-mentioned blood sugar lowering, microcirculation improvement, elimination complication, adopt the superfine grinding apparatus processing to pulverize again; Make the powder grain diameter of pulverizing reach standby between 1～160 micron; Make again in every 100CC oral liquid for decreasing blood sugar of processing and contain 1-55g glucomannan powder, 3～15g xylosic alcohol powder; Its raw material sum is 100% ratio; After in above 72g raw material, adding the 90CC distilled water earlier again; Through abundant stirring and evenly mixing after-filtration, again distilled water is added to 100CC in the stock solution after filtration; Get the stock solution embedding at last, irradiation sterilization, after the check, packed products.
5. the health promoting wine made of the earthworm protein of the described a kind of blood sugar lowering of claim 3, blood fat reducing, blood pressure lowering, microcirculation improvement, elimination diabetic complication, it is characterized in that, the described Lumbricus of selecting for use began to feed Cassia sophera Linn seed powder or Rhizoma Dioscoreae powder or Radix Et Rhizoma Fagopyri Tatarici powder or oatmeal or Abelmoschus esculentus powder in advance in 30 days, continue to feed the raw material that is elected to be the preparation earthworm protein after 30 days, described Cassia sophera Linn seed powder or Rhizoma Dioscoreae powder or Radix Et Rhizoma Fagopyri Tatarici powder or oatmeal or Abelmoschus esculentus powder are that Cassia sophera Linn seed, Rhizoma Dioscoreae, Radix Et Rhizoma Fagopyri Tatarici, Herba bromi japonici, Abelmoschus esculentus drying are crushed to the powder that 350～500 orders are made by super micron mill; The Lumbricus of described nursing after full 30 days cleaned, and it is 4～5.8 water 10～15 minutes that Lumbricus is put into pH value, adds 50g Sal simultaneously, and illumination simultaneously 2-3 hour impels Lumbricus to get rid of in the body dirt and cleans the Lumbricus body surface; Lumbricus alive after cleaning is carried out drip washing, use 28 ℃ warm water washing, wash away the Lumbricus after the excessive moisture of outer surface and impurity obtain handling; Lumbricus after the above-mentioned processing adopts 55 degree cereal wines to soak or volume ratio is 1: 1 55 degree cereal wines and the mixed liquid dipping of 20 degree red wines, add Fructus Jujubae when soaking, Fructus Lycii, Arillus Longan, the supernatant that 11 days impurity screenings of the Cassia sophera Linn stem and leaf bubble of oven dry obtain, add described earthworm protein powder again and be described health promoting wine, wherein infusion proportioning is that 55 degree cereal wine 10kg or volume ratio are 1: 1 55 degree cereal wines and the mixed liquor 10kg of 20 degree red wines, Lumbricus 1kg after the described processing, Fructus Jujubae 300g, Fructus Lycii 1kg, Arillus Longan 100g, the Cassia sophera Linn stem and leaf 100g of oven dry, described earthworm protein powder 50g.
6. the purposes of the earthworm protein of the described a kind of blood sugar lowering of claim 1, blood fat reducing, blood pressure lowering, microcirculation improvement, elimination diabetic complication is characterized in that, it is used to prepare the medicament for the treatment of or prevent diabetes.
7. the purposes of the earthworm protein of the described a kind of blood sugar lowering of claim 1, blood fat reducing, blood pressure lowering, microcirculation improvement, elimination diabetic complication is characterized in that, it is used to prepare food or the health product for the treatment of or prevent diabetes.
8. the purposes of the earthworm protein of the described a kind of blood sugar lowering of claim 1, blood fat reducing, blood pressure lowering, microcirculation improvement, elimination diabetic complication is characterized in that, it is used to prepare the medicament for the treatment of or the prevention nephrotic syndrome.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310173903.5A CN103263660B (en) | 2013-05-13 | 2013-05-13 | Earthworm protein for reducing blood sugar and blood pressure and eliminating diabetic complications and application pf earthworm protein |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310173903.5A CN103263660B (en) | 2013-05-13 | 2013-05-13 | Earthworm protein for reducing blood sugar and blood pressure and eliminating diabetic complications and application pf earthworm protein |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103263660A true CN103263660A (en) | 2013-08-28 |
| CN103263660B CN103263660B (en) | 2015-05-20 |
Family
ID=49007353
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310173903.5A Expired - Fee Related CN103263660B (en) | 2013-05-13 | 2013-05-13 | Earthworm protein for reducing blood sugar and blood pressure and eliminating diabetic complications and application pf earthworm protein |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103263660B (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104224846A (en) * | 2013-09-03 | 2014-12-24 | 脇制药株式会社 | Method for making earthworm dry powder |
| CN107736607A (en) * | 2017-10-30 | 2018-02-27 | 中盐工程技术研究院有限公司 | A kind of earthworm subtracts sodium seasoning salt and preparation method thereof |
| CN107927764A (en) * | 2017-11-20 | 2018-04-20 | 黑龙江美丽石岛科技开发有限公司 | A kind of health food containing sea cucumber extract and earthworm albumen powder and preparation method thereof |
| CN109420155A (en) * | 2017-08-24 | 2019-03-05 | 熊小利 | Hypoglycemic improves ground dragon protein and its application that diabetic complication is eliminated in microcirculation |
| CN109771633A (en) * | 2019-03-15 | 2019-05-21 | 湖南补天药业股份有限公司 | A kind of cream taste and preparation method thereof with function of blood sugar reduction |
| CN111053248A (en) * | 2018-10-16 | 2020-04-24 | 东莞市维康医疗投资有限公司 | Earthworm protein oral liquid and preparation method thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6475431A (en) * | 1987-09-17 | 1989-03-22 | Hisashi Mihara | Novel protease preparation |
| CN1037345A (en) * | 1988-04-04 | 1989-11-22 | 牛勃 | The preparation of ERTHWORM THROM BOLYTIC ENZYME and authentication method thereof |
| CN1539464A (en) * | 2003-10-30 | 2004-10-27 | 军 解 | Capsule of Chinese traditional medicine for treating thrombus |
| WO2005117895A2 (en) * | 2004-06-04 | 2005-12-15 | Boehringer Ingelheim International Gmbh | Compositions comprising meloxicam |
| CN101760454A (en) * | 2009-12-11 | 2010-06-30 | 青岛国大药业有限公司 | Preparation method of Lumbrokinase |
| CN102551065A (en) * | 2012-03-19 | 2012-07-11 | 肖梅芬 | Blood sugar reducing food series |
-
2013
- 2013-05-13 CN CN201310173903.5A patent/CN103263660B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6475431A (en) * | 1987-09-17 | 1989-03-22 | Hisashi Mihara | Novel protease preparation |
| CN1037345A (en) * | 1988-04-04 | 1989-11-22 | 牛勃 | The preparation of ERTHWORM THROM BOLYTIC ENZYME and authentication method thereof |
| CN1539464A (en) * | 2003-10-30 | 2004-10-27 | 军 解 | Capsule of Chinese traditional medicine for treating thrombus |
| WO2005117895A2 (en) * | 2004-06-04 | 2005-12-15 | Boehringer Ingelheim International Gmbh | Compositions comprising meloxicam |
| CN101760454A (en) * | 2009-12-11 | 2010-06-30 | 青岛国大药业有限公司 | Preparation method of Lumbrokinase |
| CN102551065A (en) * | 2012-03-19 | 2012-07-11 | 肖梅芬 | Blood sugar reducing food series |
Non-Patent Citations (1)
| Title |
|---|
| 樊文玲等: "超滤膜分离地龙匀浆液中蛋白物质的操作条件优化研究", 《中成药》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104224846A (en) * | 2013-09-03 | 2014-12-24 | 脇制药株式会社 | Method for making earthworm dry powder |
| CN109420155A (en) * | 2017-08-24 | 2019-03-05 | 熊小利 | Hypoglycemic improves ground dragon protein and its application that diabetic complication is eliminated in microcirculation |
| CN107736607A (en) * | 2017-10-30 | 2018-02-27 | 中盐工程技术研究院有限公司 | A kind of earthworm subtracts sodium seasoning salt and preparation method thereof |
| CN107927764A (en) * | 2017-11-20 | 2018-04-20 | 黑龙江美丽石岛科技开发有限公司 | A kind of health food containing sea cucumber extract and earthworm albumen powder and preparation method thereof |
| CN111053248A (en) * | 2018-10-16 | 2020-04-24 | 东莞市维康医疗投资有限公司 | Earthworm protein oral liquid and preparation method thereof |
| CN109771633A (en) * | 2019-03-15 | 2019-05-21 | 湖南补天药业股份有限公司 | A kind of cream taste and preparation method thereof with function of blood sugar reduction |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103263660B (en) | 2015-05-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103239706B (en) | Earthworm protein for reducing blood glucose, improving microcirculation and eliminating diabetic complication and application thereof | |
| CN103263660B (en) | Earthworm protein for reducing blood sugar and blood pressure and eliminating diabetic complications and application pf earthworm protein | |
| CN107929420A (en) | Improve energy and blood of human body function and improve the Chinese medicine composition and detection method of immunity | |
| CN103027155A (en) | Mulberry leaf lipid-reducing pressure-reducing health-care tea and preparation method thereof | |
| CN106806643A (en) | A kind of Chinese medicine composition for treating diabetes | |
| CN106244371A (en) | A kind of blood sugar lowering Rhizoma Polygonati grape health care wine and production technology thereof | |
| CN101664180B (en) | Health-care nutritional complexing agent with health effect and preparation method thereof | |
| CN103202470A (en) | Health food for reducing blood sugar in auxiliary manner and preparation method of health food | |
| CN115137069A (en) | Lactobacillus delbrueckii subspecies bulgaricus SF-L-18 fermented product and preparation method and application thereof | |
| KR20170122655A (en) | Composition for preventing and treating vascular disorders | |
| CN107095300A (en) | A kind of biological nutrition compound composition of auxiliary treatment diabetes | |
| CN103893620A (en) | Chinese medicinal composition for preventing and treating diabetes and preparation method thereof | |
| CN102552435B (en) | Health-care medicinal formula for improving alimentary anemia and enhancing immunity | |
| CN104739916A (en) | Alpha-glucosidase active inhibitor as well as preparation method and application thereof | |
| CN107812115A (en) | A kind of Chinese medicine composition for treating diabetes | |
| CN114747766A (en) | Health-care food composition capable of reducing uric acid and preparation method and application thereof | |
| CN102988747B (en) | Aristida triseta electuary for reducing hypertension, hyperglycemia and hyperlipidemia and preparation method thereof | |
| CN105055967B (en) | A kind of complex health care product and preparation method with auxiliary hyperglycemic function | |
| CN112843099A (en) | Method for extracting cordyceps sinensis mycelia with effects of moistening lung and tonifying kidney | |
| CN103340991B (en) | Oral liquid for nursing diabetics and preparation method thereof | |
| CN102302212A (en) | Anti-aging and life-increasing beverage with effect of restoring youthful energy | |
| CN106138900A (en) | Auxiliary treats hyperglycemia and the compositions of diabetic complication and application thereof | |
| CN1284848C (en) | Dry red persimmon wine and its brewing method | |
| CN104740345B (en) | Treat Chinese medicine composition of diabetes and preparation method thereof | |
| CN109420155A (en) | Hypoglycemic improves ground dragon protein and its application that diabetic complication is eliminated in microcirculation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150520 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |