CN103251741B - Chinese medicine composition for treating toxoplasmosisinswine, and preparation method and application thereof - Google Patents
Chinese medicine composition for treating toxoplasmosisinswine, and preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses a Chinese medicine composition for treating toxoplasmosisinswine, and a preparation method and application thereof. The preparation method of the Chinese medicine composition disclosed by the invention comprises the following steps of: decocting four kinds of traditional Chinese medicines consisting of negundo chastetree leaves, Chinese wampee leaves, inonotus obliquus and liquorice by adding distilled water for 1-3 times, mixing decoctions, concentrating to be thick, adding ethanol with the concentration of 90-95% to adjust alcohol concentration to be 60-70%, precipitating, filtering, concentrating filtrate, adding ethanol with the concentration of 90-95% again into the concentrated liquid medicine to adjust alcohol concentration to be 75-80%, precipitating, filtering, concentrating filtrate, refrigerating and precipitating concentrated liquid medicine by adding distilled water, filtering, and concentrating to obtain liquid medicine. The curative rate of the Chinese medicine composition disclosed by the invention on toxoplasmosisinswine is above 85%; and the Chinese medicine composition has the advantages of low side effect and low use amount and is safe and convenient to use and rapid to absorb.
Description
Technical field
The invention belongs to field of traditional Chinese medicine pharmacy, be specifically related to a boar and apply in the herbal medicine thing of preparation treatment pig toxoplasmosis with Chinese medicine composition and preparation method thereof and this Chinese medicine composition.
Background technology
Toxoplasmosis is a kind of Amphixenosis, and host type is very extensive, and humans and animals all can infection morbidity, but the sickness rate of pig is higher, and nineteen seventies is just familiar with the cause of disease of primary disease.Sulfa drugs is used in treatment to primary disease always, though there is certain effect, sulfa drugs intramuscular injection is larger to tissue irritation, and the relatively more tired lock of intravenous injection, the course for the treatment of is long.And sulfa drugs is also larger to liver, kidney side effect, if ill domestic animal liver, renal function are bad, can not use sulfa drugs, life-time service sulfa drugs, can make some antibacterial produce drug resistance simultaneously, and this is just for treatment primary disease brings difficulty.After sulfa drugs is used, in livestock products, there is drug residue, directly the health of harm humans.
Summary of the invention
The object of the present invention is to provide a kind of Chinese medicine composition for the treatment of pig toxoplasmosis.
Another object of the present invention is to provide the preparation method of above-mentioned Chinese medicine composition.
A further object of the invention is to provide above-mentioned Chinese medicine composition and applies in the herbal medicine thing of preparation treatment pig toxoplasmosis.
The object of the invention is to realize by following technical measures:
Treat a Chinese medicine composition for pig toxoplasmosis, said composition is made by the crude drug of following weight portion: Folium Viticis Negundo 15～35 weight portions, Folium clausenae lansii 15～35 weight portions, Phaeoporus obliquus 8～14 weight portions, Radix Glycyrrhizae 1～6 weight portion.
Preferably said composition is made by the crude drug of following weight portion: Folium Viticis Negundo 15～30 weight portions, Folium clausenae lansii 15～30 weight portions, Phaeoporus obliquus 8～12 weight portions, Radix Glycyrrhizae 2～6 weight portions.
The dosage form that described Chinese medicine composition adopts can be injection or oral liquid.
The Chinese medicine composition of above-mentioned treatment pig toxoplasmosis prepares through the following steps:
A. after Folium Viticis Negundo, Folium clausenae lansii, Phaeoporus obliquus and Radix Glycyrrhizae adding distil water being soaked, decoct 1～3 time, collecting decoction is also concentrated into thick shape concentrated solution;
B. to add in concentrated solution concentration be 90%～95% ethanol to be adjusted to determining alcohol be 60%～70%, fully stirring and evenly mixing, staticly settles more than 24 hours, filter, filtrate recycling ethanol, reclaiming medicinal liquid after ethanol, to add concentration be that to be adjusted to determining alcohol be 75%～80% to 90%～95% ethanol again, stir evenly, staticly settle more than 24 hours, filter, filtrate recycling ethanol reconcentration is extremely without alcohol taste, the cold preservation of medicinal liquid adding distil water staticly settles more than 24 hours, filter, filtrate is concentrated, preparation.Cold preservation described in step " b " staticly settles more than 24 hours, filters, and after filtrate is concentrated, obtains medicinal liquid,, preparation; Also can add the chlorobutanol of medicinal liquid weight 0.1%～1.0% as analgesic.
The Chinese medicine composition preparation method of described treatment pig toxoplasmosis, the method comprises the following steps:
A. after Folium Viticis Negundo, Folium clausenae lansii, Phaeoporus obliquus and Radix Glycyrrhizae adding distil water being soaked, decoct 1～3 time, collecting decoction is also concentrated into thick shape concentrated solution;
B. to add in concentrated solution concentration be 90%～95% ethanol to be adjusted to determining alcohol be 60%～70%, fully stirring and evenly mixing, staticly settles more than 24 hours, filter, filtrate recycling ethanol, reclaiming medicinal liquid after ethanol, to add concentration be that to be adjusted to determining alcohol be 75%～80% to 90%～95% ethanol again, stir evenly, staticly settle more than 24 hours, filter, filtrate recycling ethanol reconcentration is extremely without alcohol taste, the cold preservation of medicinal liquid adding distil water staticly settles more than 24 hours, filter, filtrate is concentrated, preparation.
Wherein, the cold preservation described in step " b " staticly settles more than 24 hours, filters, and after filtrate is concentrated, obtains medicinal liquid, adds the chlorobutanol of medicinal liquid weight 0.1%～1.0%.
The preparation method of the Chinese medicine composition of described treatment pig toxoplasmosis, specifically can follow these steps to preparation:
A. the distilled water immersion of 3～4 times of Folium Viticis Negundos, Folium clausenae lansii, Phaeoporus obliquus, 4 kinds of medical material gross weights of Radix Glycyrrhizae is decocted after 24～30 hours 3 times, after boiling, maintain 0.5～1 hour at every turn, collecting decoction also concentratedly obtains thick shape concentrated solution;
B. to adding concentration in concentrated solution, be that to be adjusted to determining alcohol be 60%～70% for 95% ethanol, abundant stirring and evenly mixing, staticly settle 24～48 hours, filter, filtrate recycling ethanol, reclaiming medicinal liquid after ethanol, to add concentration be that to be adjusted to determining alcohol be 75%～80% for 95% ethanol again, stir evenly, staticly settle 24～48 hours, filter, filtrate recycling ethanol reconcentration is extremely without alcohol taste, the cold preservation of medicinal liquid adding distil water staticly settles 24～48 hours, filter, filtrate is concentrated, obtaining concentration is every milliliter of medicinal liquid that contains 1 gram of crude drug amount, 0.1%～1.0% chlorobutanol that adds medicinal liquid weight, adjust pH to 7, make injection or oral liquid.
Chinese medicine composition of the present invention application in the herbal medicine thing of preparation treatment pig toxoplasmosis has significant effect.
Beneficial effect of the present invention compared with the prior art:
The present invention draws the marrow of traditional Chinese veterinary medicine, reasonable recipe, determined curative effect.In compositions Folium Viticis Negundo Vitex negundo L. there is treating malaria, killing parasites for relieving itching, the thermolysis of inducing sweat.Folium clausenae lansii Clausena lansium (Lour.) Skeels has treating malaria, the heat radiation of inducing sweat, promoting the circulation of QI and reducing phlegm, diuresis, Detoxication.Phaeoporus obliquus Inonotus obliquus (Fr.) Pilat control digestive tract disease, cardiovascular disease and the tumor of being widely used among the people.Radix Glycyrrhizae Glycyrrhiza uralensis Fisch. have QI invigorating and in, heat-clearing and toxic substances removing, coordinating the actions of various ingredients in a prescription, sweet slow lung moistening, expelling phlegm for arresting cough effect.4 kinds of compatibility of drugss, reach the effect for the treatment of both the principal and secondary aspects of a disease.Treatment verminosis of animal, be main substantially, but pig toxoplasma is cytozoicus with chemical drugs now, belong to protozoacide, only have at present sulfa drugs therapeutic effect better, and sulfa drugs treatment toxoplasmosis, untoward reaction is more, has a lot of side effect, easily develops immunity to drugs.With the present invention, treat pig toxoplasmosis, curative effect and sulfa drugs are close, and have no side effect, and welcome by raiser.
The present invention is pure Chinese medicine formula, and side effect is little, uses safety, and cure rate reaches more than 85%, is difficult for recurrence, has avoided the generation of residue of veterinary drug and social effects of pollution.
With regard to clinical effectiveness of the present invention, further illustrate curative effect of the present invention below:
One, present composition animal inoculation test
1, medicine: this test be take the present invention as test drug, sulfamonomethoxine sodium injection is Western medicine contrast.
2, Toxoplasma gondii kind: RH strain of Toxoplasma gondii source Yangzhou University veterinary college.
3, experimental animal: Kunming white mice, clean level, 180,20～22 grams of body weight, male and female half and half, purchased from Yangzhou University.Wherein, 144 for Drug therapy test, another 36 for median lethal dose(LD 50) determination test.
4, test grouping: be divided at random 4 groups, 36 every group, wherein 18 for adding up cure rate, 6 for counting polypide number, and 12 for measuring SOD activity.The 1st group of inoculation polypide treated by medicine of the present invention (preparing according to embodiment 1 method) gavage after 4 hours, every 500 milligrams of crude drug/0.5 milliliter, 0.5 milliliter every, a gavage, every day 2 times, medication 5 days.The 2nd group of inoculation polypide used sulfamonomethoxine sodium injection (Sulfamonomethoxine Sodium Injection after 4 hours, lot number: veterinary drug word (2006) 100171616, Jiangsu south agriculture high-tech animal pharmaceutical estate company limited, specification: 10 milliliters containing 1000 milligrams) gavage treatment, is dissolved in normal saline, by Mouse Weight, is mixed with 200 mgs/kg of dosages, 0.5 milliliter every, a gavage, every day 2 times, medication 5 days.The 3rd group of not medication, inoculation polypide by 0.5 ml physiological saline gavage, every day 2 times, was used in conjunction 5 heaven-made positive controls after 4 hours.Do not inoculate polypide for the 4th group, not medication, by 0.5 ml physiological saline gavage, every day 2 times, is used in conjunction 5 days, makes negative control.Therapeutic outcome is in Table 1.
Table 1 white mice artificial vaccination toxoplasma therapeutic test result
5, test method:
(1) polypide recovery: take out worm kind from liquid nitrogen, the warm water that is placed in rapidly 37 ℃ is recovered, through intraperitoneal inoculation mice, continuous passage 3 times, until worm strain stable after, collect peritoneal fluid standby.
(2) median lethal dose(LD 50) is measured: standby peritoneal fluid is counted to polypide content through blood counting chamber, inoculate respectively 6 groups, every group 6, by 5 of every group of inoculations/only, 10/only, 15/only, 20/only, 25/only, the peritoneal fluids of 30/polypides, observe 20 days, see its death condition, its median lethal dose(LD 50) is 10/polypides.During formal inoculation test, except negative control group, all the other respectively organize 20/0.3 milliliter of every mouse peritoneal inoculation toxoplasma tachyzoite RH strain, 0.3 milliliter/of negative control group mouse peritoneal injecting normal saline.
(3) administration time: mice is treated with different pharmaceutical after 4 hours at inoculation toxoplasma, in experimentation, observe and record case every day, inoculates 20 days and finish experiment.
(4) SOD determination of activity: after inoculation toxoplasma, every group was used 6 mices take a blood sample and prepare serological method and measure activity of SOD in serum by plucking eyeball, the results are shown in Table 2 in the 3rd day, the 6th day.
(5) polypide counting: each group after inoculation toxoplasma when morbidity in the 7th day random acquisition 6 mouse peritoneal liquid, microscopies count polypide quantity respectively, the results are shown in Table 3.
Table 2 is respectively organized mouse serum SOD determination of activity
Under morbid state, in Mice Body, for removing the free radical producing, make SOD(serum superoxide dismutases in early days) the temporary rising of active appearance; In the later stage, consume SOD for removing part free radical in body on the one hand, and existing SOD will be subject to the destruction of free radical on the other hand, so SOD activity will significantly decline.As shown in Table 2, after using medicine of the present invention, can improve and maintain SOD activity in body, reduce the damage that radical pair body produces.
Table 3 is respectively organized intraperitoneal mouse liquid tachyzoite average
Two, the present invention observes pig toxoplasmosis clinical treatment result
Annual 5～the JIUYUE of pig toxoplasmosis is morbidity season, of all ages, sex, kind.Main clinical manifestation is fervescence, and cough and asthma, is hard and dry, poor appetite or absolutely useless.With medicinal liquid of the present invention (preparing according to embodiment 2 methods), in Jiangsu, morbidity pig has been carried out to Drug therapy, by pig body weight dosage be 200 milligrams of crude drugs/kilogram, intramuscular injection, every day 2 times, is used in conjunction 5 (medicine specification: 1 milliliter containing 1 gram of crude drug amount).Treat altogether 1183, cure 1024, cure rate is more than 85%, specifically in Table 4.
The curative effect statistics of table 4 clinical treatment pig toxoplasmosis
The specific embodiment:
Below in conjunction with specific embodiment, the invention will be further described:
Embodiment 1:
Prescription: 15 grams of Folium Viticis Negundos; 17 grams of Folium clausenae lansives; 8 grams of Phaeoporus obliquus; 5 grams, Radix Glycyrrhizae.
Concrete preparation process is as follows:
A. to Folium Viticis Negundo, Folium clausenae lansii, Phaeoporus obliquus, Radix Glycyrrhizae, mix the distilled water immersion of 3 times after 24 hours that adds 4 taste Chinese medicine gross weights in medical material, decoct 3 times, after boiling, maintain 0.5 hour at every turn, collecting decoction is also concentrated into thick shape concentrated solution.
B. to adding concentration in concentrated solution, be that to be adjusted to determining alcohol be 60% to 95% ethanol, abundant stirring and evenly mixing, staticly settle 24 hours, filter, filtrate recycling ethanol, reclaiming medicinal liquid after ethanol, to add concentration be that to be adjusted to determining alcohol be 75% for 95% ethanol again, stir evenly, staticly settle 48 hours, filter, filtrate recycling ethanol reconcentration is extremely without alcohol taste, in the medicinal liquid after concentrated, add 0 ℃～4 ℃ cold preservations of 4 times of amount distilled water of medicinal liquid, staticly settle 48 hours, filter, filtrate is concentrated into every milliliter containing 1 gram of crude drug amount, obtains the medicinal liquid that Folium Viticis Negundo, Folium clausenae lansii, Phaeoporus obliquus, Radix Glycyrrhizae make.
C. the chlorobutanol that adds medicinal liquid weight 0.3% in the medicinal liquid making to Folium Viticis Negundo, Folium clausenae lansii, Phaeoporus obliquus, Radix Glycyrrhizae, adjusts pH to 7, makes oral liquid, flowing steam sterilization and get final product.
Embodiment 2:
Prescription: 30 grams of Folium Viticis Negundos; 26 grams of Folium clausenae lansives; 12 grams of Phaeoporus obliquus; 2 grams, Radix Glycyrrhizae.
A. the distilled water immersion that mixes to Folium Viticis Negundo, Folium clausenae lansii, Phaeoporus obliquus, Radix Glycyrrhizae 3 times of amounts that add 4 taste Chinese medicine gross weights in medical material, after 30 hours, decocts 3 times, after boiling, maintains 1 hour at every turn, and collecting decoction is also concentrated into thick shape concentrated solution.
B. to adding concentration in concentrated solution, be that to be adjusted to determining alcohol be 65% to 95% ethanol, abundant stirring and evenly mixing, precipitate 48 hours, filter, filtrate recycling ethanol, reclaiming medicinal liquid after ethanol, to add concentration be that to be adjusted to medicinal liquid determining alcohol be 75% for 95% ethanol again, stir evenly, staticly settle 48 hours, filter, filtrate recycling ethanol reconcentration is extremely without alcohol taste, and the medicinal liquid after concentrated adds 2 times of amount distilled water cold preservations precipitations of medicinal liquid 24 hours, filter, filtrate is concentrated into every milliliter and measures containing 1 gram of crude drug the medicinal liquid that Folium Viticis Negundo, Folium clausenae lansii, Phaeoporus obliquus, Radix Glycyrrhizae make.
C. the medicinal liquid making to Folium Viticis Negundo, Folium clausenae lansii, Phaeoporus obliquus, Radix Glycyrrhizae adds the chlorobutanol of medicinal liquid weight 0.6%, adjusts pH to 7, makes injection, flowing steam sterilization and get final product.
Embodiment 3:
Prescription: 35 grams of Folium Viticis Negundos; 35 grams of Folium clausenae lansives; 14 grams of Phaeoporus obliquus; 4 grams, Radix Glycyrrhizae.
A. to Folium Viticis Negundo, Folium clausenae lansii, Phaeoporus obliquus, Radix Glycyrrhizae, mix 4 times of distilled water that add 4 taste medical material gross weights in medical material, soak and decoct 3 times after 27 hours, after boiling, maintain 1 hour at every turn, collecting decoction is also concentrated into thick shape concentrated solution;
B. to adding concentration in concentrated solution, be that to be adjusted to determining alcohol be 70% for 95% ethanol, abundant stirring and evenly mixing, staticly settle 48 hours, filter, filtrate recycling ethanol, reclaiming medicinal liquid after ethanol, to add concentration be that to be adjusted to medicinal liquid determining alcohol be 80% for 95% ethanol again, stir evenly, staticly settle 48 hours, filter, filtrate recycling ethanol reconcentration is extremely without alcohol taste, and the medicinal liquid after concentrated adds 2 times of weight distilled water cold preservations precipitations of medicinal liquid 48 hours, filter, filtrate is concentrated into every milliliter and measures containing 1 gram of crude drug the medicinal liquid that Folium Viticis Negundo, Folium clausenae lansii, Phaeoporus obliquus, Radix Glycyrrhizae make;
C. the chlorobutanol that adds medicinal liquid weight 0.9% in the medicinal liquid making to Folium Viticis Negundo, Folium clausenae lansii, Phaeoporus obliquus, Radix Glycyrrhizae, adjusts pH to 7, makes injection, flowing steam sterilization and get final product.
Claims (6)
1. treat a Chinese medicine composition for pig toxoplasmosis, it is characterized in that said composition made by the crude drug of following weight portion: Folium Viticis Negundo 15～30 weight portions, Folium clausenae lansii 15～30 weight portions, Phaeoporus obliquus 8～12 weight portions, Radix Glycyrrhizae 2～6 weight portions.
2. the Chinese medicine composition for the treatment of pig toxoplasmosis according to claim 1, is characterized in that the dosage form that described Chinese medicine composition adopts is injection or oral liquid.
3. the preparation method of the Chinese medicine composition for the treatment of claimed in claim 1 pig toxoplasmosis, is characterized in that the method is:
A. after Folium Viticis Negundo, Folium clausenae lansii, Phaeoporus obliquus and Radix Glycyrrhizae adding distil water being soaked, decoct 1～3 time, collecting decoction is also concentrated into thick shape concentrated solution;
B. to add in concentrated solution concentration be 90%～95% ethanol to be adjusted to determining alcohol be 60%～70%, fully stirring and evenly mixing, staticly settles more than 24 hours, filter, filtrate recycling ethanol, reclaiming medicinal liquid after ethanol, to add concentration be that to be adjusted to determining alcohol be 75%～80% to 90%～95% ethanol again, stir evenly, staticly settle more than 24 hours, filter, filtrate recycling ethanol reconcentration is extremely without alcohol taste, the cold preservation of medicinal liquid adding distil water staticly settles more than 24 hours, filter, filtrate is concentrated, preparation.
4. the preparation method of the Chinese medicine composition for the treatment of according to claim 3 pig toxoplasmosis, it is characterized in that the cold preservation described in step " b " staticly settles more than 24 hours, filter, after filtrate is concentrated, obtain medicinal liquid, add the chlorobutanol of medicinal liquid weight 0.1%～1.0%.
5. the preparation method of the Chinese medicine composition for the treatment of according to claim 3 pig toxoplasmosis, is characterized in that the method specifically can follow these steps to preparation:
A. get Folium Viticis Negundo, Folium clausenae lansii, Phaeoporus obliquus, 4 kinds of medical materials of Radix Glycyrrhizae, add the distilled water immersion of 3～4 times of amounts of medical material gross weight after 24～30 hours, to decoct 3 times, after boiling, maintain 0.5～1 hour at every turn, collecting decoction also concentrates and obtains thick shape concentrated solution;
B. to adding concentration in concentrated solution, be that to be adjusted to determining alcohol be 60%～70% for 95% ethanol, abundant stirring and evenly mixing, staticly settle 24～48 hours, filter, filtrate recycling ethanol, reclaiming medicinal liquid after ethanol, to add concentration be that to be adjusted to determining alcohol be 75%～80% for 95% ethanol again, stir evenly, staticly settle 24～48 hours, filter, filtrate recycling ethanol reconcentration is extremely without alcohol taste, the cold preservation of medicinal liquid adding distil water staticly settles 24～48 hours, filter, filtrate is concentrated, obtaining concentration is every milliliter of medicinal liquid that contains 1 gram of crude drug amount, 0.1%～1.0% chlorobutanol that adds medicinal liquid weight, adjust pH to 7, make injection or oral liquid.
6. the Chinese medicine composition described in claim 1 is applied in the herbal medicine thing of preparation treatment pig toxoplasmosis.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103877444B (en) * | 2014-03-15 | 2016-08-17 | 青岛绿曼生物工程有限公司 | Chinese medicine composition for the treatment of swine toxoplasmosis and preparation method thereof |
| CN104431347A (en) * | 2014-09-30 | 2015-03-25 | 全椒县大地种植专业合作社 | Feed additive for preventing and treating swine toxoplasmosis |
| MY191486A (en) * | 2016-03-11 | 2022-06-28 | Leeward Pacific Pte Ltd | Compound for use in the treatment and/or prevention of parasitic mediated diseases |
| CN106822536B (en) * | 2017-03-22 | 2018-09-11 | 闫颖颖 | A kind of Chinese medicine composition and the preparation method and application thereof for treating toxplasmosis in pigs |
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| CN1337245A (en) * | 2000-08-16 | 2002-02-27 | 钟梓光 | Cold treating medicine |
| CN102416060A (en) * | 2010-09-27 | 2012-04-18 | 姚昂 | Traditional Chinese medicine for preventing influenza |
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| CN1337245A (en) * | 2000-08-16 | 2002-02-27 | 钟梓光 | Cold treating medicine |
| CN102416060A (en) * | 2010-09-27 | 2012-04-18 | 姚昂 | Traditional Chinese medicine for preventing influenza |
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| Title |
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| 4种中药治疗急性弓形虫感染小鼠的疗效观察;王明建等;《中国兽医杂志》;20100131;第46卷(第01期);第33页左栏第3段，表1 * |
| 中药抗弓形虫感染的研究思路与方法;宋贤响等;《江西中医学院学报》;20060310;第18卷(第01期);全文 * |
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