CN103250867A - Production method of raw milk lactic acid bacteria antimicrobial peptide additive for livestock and poultry - Google Patents
Production method of raw milk lactic acid bacteria antimicrobial peptide additive for livestock and poultry Download PDFInfo
- Publication number
- CN103250867A CN103250867A CN2013101722833A CN201310172283A CN103250867A CN 103250867 A CN103250867 A CN 103250867A CN 2013101722833 A CN2013101722833 A CN 2013101722833A CN 201310172283 A CN201310172283 A CN 201310172283A CN 103250867 A CN103250867 A CN 103250867A
- Authority
- CN
- China
- Prior art keywords
- livestock
- lactic acid
- culture medium
- production method
- acid bacteria
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a production method of a raw milk lactic acid bacteria antimicrobial peptide additive for livestock and poultry, relates to a production method of fermentation in various plant feed proteins and solves problems that the production strains of the antimicrobial peptide additive for the livestock and the poultry is undefined and the activity of antimicrobial peptide is low. The production method comprises the following steps that 1, frozen Lactobacillus acidophilus strains and Lactobacillus casei are activated by MRS culture mediums and then are respectively inoculated in enrichment mediums so that the enrichment culture solution is obtained; 2, the enrichment culture solution is inoculated in a seed culture medium and then is fermented at 35-40 DEG C to obtain a seed culture solution; 3, the seed culture solution is inoculated to the fermentation culture medium again to be fermented to obtain fermentation products; and 4, the fermentation products are dried, dehydrated, crushed and mixed to obtain the lactic acid bacteria antimicrobial peptide additive. The bacteria antimicrobial peptide additive obtained by the production method disclosed by the invention has defined strains, the activity of the antimicrobial peptide is high, and the obtained bacteria antimicrobial peptide additive is mainly applied to prevention and cure of disease of digestive tract of the livestock and poultry.
Description
Technical field
The present invention relates to a kind of production method of the various plants forage protein that ferments.
Background technology
Lactobacillus acidophilus and Lactobacillus casei cheese subspecies belong to 42 kinds that U.S. FDA announces can directly feed the probio kind of raising.Probio is the active microorganism raw-food material that a class is of value to health, and probio commonly used at present has lactobacillus acidophilus and Bifidobacterium two big classes, is called as " healthy three beneficial bacterium " with Lactobacillus casei.Lactobacillus acidophilus and Lactobacillus casei cheese subspecies have significant benefit to its host's nutrition, immunity, diseases prevention etc. and give birth to effect, more and more become the focus of people's research, exploitation, production.After Metchnikoff in 1908 finds that lactobacillus can suppress harmful bacterium in the enteron aisle, each each kind that belongs to kind that the various countries scholar finds lactic acid bacteria almost successively, even each strain that can produce lactein.These lacteins become one of native peptides class anticorrisive agent and originate greatly.Lactobacillus casei can suppress and kill many spoilage organisms and the pathogenic bacteria in the food, and do not influence the food proterties, even can improve food properties, therefore it is added to can make product high-quality more in the food as leavening, and the anti-corrosive fresh-keeping in the food storage process is also played positive role.Originally discover, Lactobacillus casei cheese subspecies ATCC334 genome contains 2 series antimicrobial peptide genes bunch (Lac705beta and Lac705alpha) and 1 independent gene (Lactobacillus curvatus plasmid pRC18), lactobacillus acidophilus CGMCC103342 contains 1 antibacterial peptide gene (acd A), lactobacillus acidophilus and Lactobacillus casei cheese subspecies tunning have significant inhibitory effect to staphylococcus aureus and Escherichia coli, the shelf-life of energy significant prolongation fresh milk.
Disease of digestive tract easily takes place in livestock and poultry such as pig, chicken, as diarrhea of weaned piglets, transmissible gastroenteritis, chicken intestinal diorder, salmonellosis etc., has a strong impact on production performance, even causes death.At present, antibiotic therapy more and more is restricted, and oneself becomes the trend of prevention and disease of alimentary tract of livestock husbandry and birds such as treatment pig, chicken development green natural antibacterial substance.Breast source lactic acid bacteria antibacterial peptide has the intestines and stomach of killing cause of disease bacterium, and the balance gastrointestinal bacterial flora such as promotes to digest and assimilate at effect.At present, domestic existing antibacterial peptide product is used more and more wider in animal husbandry, but existing product also exists fermented bacterium indeterminate, technical problems such as zymotechnique instability.The antibacterial peptide bacterial classification is effectively produced in screening, and the microbial metabolism of product antibacterial peptide is regulated and control, and sets up the zymotechnique that is fit to, and is the important technological problems that needs solution during antibacterial peptide is produced.
Lactobacillus acidophilus and Lactobacillus casei cheese subspecies have stronger product acid, produce perfume, produce abilities such as sticking and antagonism pathogenic bacteria, by optimizing lactobacillus acidophilus and Lactobacillus casei cheese subspecies propagation and ferment effect and low temperature drying, can remove ANFs such as urase in the dregs of rice albuminoid, trypsin ihhibitor, phytic acid, phenols, improve the active and restricted essential amino acids content of antibacterial peptide, the molecular mass of reduction protein is improved palatability and efficient is digested and assimilated in raising.Lactobacillus acidophilus and Lactobacillus casei cheese subspecies still are rarely used in feed antibiotic health care production at present, and its fermented product has vast market prospect for disease of alimentary tract of livestock husbandry and birds control (system).
Summary of the invention
To the present invention seeks to have the problem that livestock and poultry are indeterminate with antibacterial peptide Additive Production bacterial classification and the antibacterial peptide activity is on the low side now in order solving, and the production method of livestock and poultry with newborn source lactic acid bacteria antibacterial peptide additive to be provided.
Livestock and poultry of the present invention follow these steps to realize with the production method of newborn source lactic acid bacteria antibacterial peptide additive:
One, frozen lactobacillus acidophilus bacterial classification and Lactobacillus casei cheese subspecies are activated with the MRS culture medium, then by volume the inoculum concentration of percentage composition 1%～5% with the lactobacillus acidophilus bacterial classification inoculation in proliferated culture medium A, at 35～40 ° of C bottom fermentation 35～37h, obtain lactobacillus acidophilus propagation nutrient solution, by volume the inoculum concentration of percentage composition 1%～5% is inoculated in Lactobacillus casei cheese subspecies among the proliferated culture medium B, at 35～40 ° of C bottom fermentation 35～37h, obtain Lactobacillus casei cheese subspecies propagation nutrient solution; Wherein every liter of proliferated culture medium A is made by the distilled water of 25～30g sucrose, 35～40g beef extract, 5～6gNaCl, 10～15gTween-80 and surplus, and pH is 5.0; Every liter of proliferated culture medium B is made by the distilled water of 9～10g sucrose, 25～30g soy peptone, 6～7gNaCl, 9～10gTween-80 and surplus, and pH is 6.0;
Two, the lactobacillus acidophilus propagation nutrient solution that step 1 is obtained and Lactobacillus casei cheese subspecies propagation nutrient solution respectively by volume the inoculum concentration of percentage composition 10%～20% be inoculated in the seed culture medium, at 35～40 ° of C bottom fermentation 35～37h, obtain lactobacillus acidophilus seed culture fluid and Lactobacillus casei cheese subspecies seed culture fluid, wherein every liter of seed culture medium is by 200～250g dry, 15～20g urea, the distilled water of the hot inactivated bacterial liquid of 35～40g staphylococcus aureus and surplus is made, and dregs of beans and corn protein powder that the bent mass ratio of dry is 2.0～3.0:1 are formed;
Three, the lactobacillus acidophilus seed culture fluid that respectively step 2 is obtained and Lactobacillus casei cheese subspecies the seed culture fluid by volume inoculum concentration of percentage composition 5%～20% are inoculated in the fermentation medium, spend 35～40 ° of C bottom fermentation 45～50h, obtain lactobacillus acidophilus tunning and Lactobacillus casei cheese subspecies tunning, wherein every liter of fermentation medium is made by mixed powder wood and the distilled water of 450～500g, and mixed-powder is that bean cake powder, cotton dregs powder, dish dregs of rice powder and the corn flour of 13:3:3:4 formed by mass ratio;
Four, lactobacillus acidophilus tunning and Lactobacillus casei cheese subspecies tunning are dried to water content 12%～15% respectively under 30～40 ° of C, be crushed to 35～45 orders then, 1:1 mixes tunning by mass ratio, obtains livestock and poultry with newborn source lactic acid bacteria antibacterial peptide additive.
Livestock and poultry of the present invention are raw material with the antibiotic skin additive of newborn source lactic acid bacteria with dregs of rice albuminoid and corn flour, carry out biofermentation respectively by lactobacillus acidophilus and Lactobacillus casei cheese subspecies, the Additive Production bacterial classification is clear and definite, significantly improve the active and restricted essential amino acids content of antibacterial peptide, reduce ANFs content such as urase in the dregs of rice albuminoid, trypsin ihhibitor, phytic acid, phenols, and improved little molecular mass soluble protein content.
The livestock and poultry that the present invention is obtained are raised with feeding after feed mixes by the content of 0.1～1g/ pig cock jin body weight/day with newborn source lactic acid bacteria antibacterial peptide additive, play the control disease of alimentary tract of livestock husbandry and birds, improve livestock and poultry production performance and product quality.After adding antibacterial peptide additive 24h in 4 ° of former milk of C, the thalline number descends gradually in the former milk, illustrate that the antibacterial peptide additive brings into play bacteriostasis in former milk, makes the bacterium that exists in the former milk stop breeding, even death.The present invention is mainly used in the control of disease of alimentary tract of livestock husbandry and birds.
Description of drawings
Fig. 1 is the livestock and poultry that obtain of embodiment one with the inhibition of newborn source lactic acid bacteria antibacterial peptide additive to 4 ° of former milk totals number of bacteria of C, ■ represents former milk blank, ● expression Lactobacillus casei cheese subspecies are to the inhibition of 4 ° of former milk totals number of bacteria of C, and ▲ expression lactobacillus acidophilus bacterial classification is to the inhibition of 4 ° of former milk totals number of bacteria of C;
Fig. 2 is the livestock and poultry that obtain of embodiment one with the inhibition of newborn source lactic acid bacteria antibacterial peptide additive to 25 ° of former milk totals number of bacteria of C, ■ represents former milk blank, ◆ expression Lactobacillus casei cheese subspecies are to the inhibition of 25 ° of former milk totals number of bacteria of C, and ▲ expression lactobacillus acidophilus bacterial classification is to the inhibition of 25 ° of former milk totals number of bacteria of C.
The specific embodiment
The specific embodiment one: the present embodiment livestock and poultry follow these steps to realize with the production method of newborn source lactic acid bacteria antibacterial peptide additive:
One, frozen lactobacillus acidophilus bacterial classification and Lactobacillus casei cheese subspecies are activated with the MRS culture medium, then by volume the inoculum concentration of percentage composition 1%～5% with the lactobacillus acidophilus bacterial classification inoculation in proliferated culture medium A, at 35～40 ° of C bottom fermentation 35～37h, obtain lactobacillus acidophilus propagation nutrient solution, by volume the inoculum concentration of percentage composition 1%～5% is inoculated in Lactobacillus casei cheese subspecies among the proliferated culture medium B, spend 35～40 ° of C bottom fermentation 35～37h, obtain Lactobacillus casei cheese subspecies propagation nutrient solution; Wherein every liter of proliferated culture medium A is made by the distilled water of 25～30g sucrose, 35～40g beef extract, 5～6gNaCl, 10～15gTween-80 and surplus, and pH is 5.0; Every liter of proliferated culture medium B is made by the distilled water of 9～10g sucrose, 25～30g soy peptone, 6～7gNaCl, 9～10gTween-80 and surplus, and pH is 6.0;
Two, the lactobacillus acidophilus propagation nutrient solution that step 1 is obtained and Lactobacillus casei cheese subspecies propagation nutrient solution respectively by volume the inoculum concentration of percentage composition 10%～20% be inoculated in the seed culture medium, at 35～40 ° of C bottom fermentation 35～37h, obtain lactobacillus acidophilus seed culture fluid and Lactobacillus casei cheese subspecies seed culture fluid, wherein every liter of kind in culture medium by 200～250g dry, 15～20g urea, the distilled water of the hot inactivated bacterial liquid of 35～40g staphylococcus aureus and surplus is made, and dry is that dregs of beans and the corn protein powder of 2.8～3.0:1 formed by mass ratio;
Three, the lactobacillus acidophilus seed culture fluid that respectively step 2 is obtained and Lactobacillus casei cheese subspecies the seed culture fluid by volume inoculum concentration of percentage composition 5%～20% are inoculated in the fermentation medium, at 35～40 ° of C bottom fermentation 45～50h, obtain lactobacillus acidophilus tunning and Lactobacillus casei cheese subspecies tunning, wherein every liter of fermentation medium is made by mixed-powder and the distilled water of 450～500g, and mixed-powder is that bean cake powder, cotton dregs powder, dish dregs of rice powder and the corn flour of 13:3:3:4 formed by mass ratio;
Four, lactobacillus acidophilus tunning and Lactobacillus casei cheese subspecies tunning are dried to water content 12%～15% respectively under 30～40 ° of C, be crushed to 35～45 orders then, 1:1 mixes tunning by mass ratio, obtains livestock and poultry with newborn source lactic acid bacteria antibacterial peptide additive.
Present embodiment lactobacillus acidophilus bacterial classification (Lacrobacillusacidophilus) NCFM, deposit number is CGMCC1.03342; Lactobacillus casei cheese subspecies (Lactobacillus casei subs.casei), deposit number is ATCC334.Used lactobacillus acidophilus bacterial classification and Lactobacillus casei cheese subspecies can obtain by purchase.
Present embodiment is raw material with dregs of rice albuminoid and corn flour etc., by lactobacillus acidophilus and the Lactobacillus casei cheese subspecies capable biofermentation that spouts respectively, significantly improve the active and restricted essential amino acids content of antibacterial peptide, ANFs content such as the urase in the remarkable reduction dregs of rice albuminoid, trypsin ihhibitor, phytic acid, phenols, and improve little molecular mass soluble protein content.
The specific embodiment two: present embodiment and the specific embodiment one are different be step 1 by volume the inoculum concentration of percentage composition 5% with the lactobacillus acidophilus bacterial classification inoculation in proliferated culture medium A.Other step and parameter are identical with the specific embodiment one.
The specific embodiment three: what present embodiment was different with the specific embodiment one or two is that every liter of proliferated culture medium A of step 1 is made by the distilled water of 30g sucrose, 40g beef extract, 5gNaCl, 15gTween-80 and surplus, and pH is 5.0.Other step and parameter are identical with the specific embodiment one or two.
The specific embodiment four: what present embodiment was different with one of specific embodiment one to three is that every liter of proliferated culture medium B of step 1 is made by the distilled water of 10g sucrose, 30g soy peptone, 7gNaCl, 10gTween-80 and surplus, and pH is 6.0.Other step and parameter are identical with one of specific embodiment one to three.
The specific embodiment five: present embodiment is different with one of specific embodiment one to four be step 2 lactobacillus acidophilus propagation nutrient solution and Lactobacillus casei cheese subspecies propagation nutrient solution respectively by volume the inoculum concentration of percentage composition 10% be inoculated in the seed culture medium.Other step and parameter are identical with one of specific embodiment one to four.
The specific embodiment six: what present embodiment was different with one of specific embodiment one to five is that every liter of seed culture medium of step 2 is by the 200g dry, 20g urea, the distilled water of the hot inactivated bacterial liquid of 40g staphylococcus aureus and surplus is made, and dry is that dregs of beans and the corn protein powder of 3.0:1 formed by mass ratio.Other step and parameter are identical with one of specific embodiment one to five.
The specific embodiment seven: present embodiment is different with one of specific embodiment one to six is that the granularity of step 2 dregs of beans and corn protein powder is 40 orders.Other step and parameter are identical with one of specific embodiment one to six.
The specific embodiment eight: present embodiment is different with one of specific embodiment one to seven be step 3 lactobacillus acidophilus seed culture fluid and Lactobacillus casei cheese subspecies seed culture fluid by volume the inoculum concentration of percentage composition 10% be inoculated in the fermentation medium.Other step and parameter are identical with one of specific embodiment one to seven.
Embodiment one: the present embodiment livestock and poultry follow these steps to realize with the production method of newborn source lactic acid bacteria antibacterial peptide additive:
One, frozen lactobacillus acidophilus bacterial classification and Lactobacillus casei cheese subspecies are activated with the MRS culture medium, then by volume the inoculum concentration of percentage composition 5% with the lactobacillus acidophilus bacterial classification inoculation in proliferated culture medium A, at 37 ° of C bottom fermentation 36h, obtain lactobacillus acidophilus propagation nutrient solution, by volume the inoculum concentration of percentage composition 5% is inoculated in Lactobacillus casei cheese subspecies among the proliferated culture medium B, at 37 ° of C bottom fermentation 36h, obtain Lactobacillus casei cheese subspecies propagation nutrient solution; Wherein every liter of proliferated culture medium A is made by the distilled water of 30g sucrose, 40g beef extract, 5gNaCl, 15g Tween-80 and surplus, and pH is 5.0; Every liter of proliferated culture medium B is made by the distilled water of 10g sucrose, 30g soy peptone, 7gNaCl, 10g Tween-80 and surplus, and pH is 6.0;
Two, the lactobacillus acidophilus that step 1 obtained propagation nutrient solution and Lactobacillus casei cheese subspecies propagation nutrient solution respectively by volume the inoculum concentration of percentage composition 10% be inoculated in the seed culture medium, at 37 ° of C bottom fermentation 36h, obtain lactobacillus acidophilus seed culture fluid and Lactobacillus casei cheese subspecies seed culture fluid, wherein every liter of seed culture medium is by the 200g dry, 20g urea, the distilled water of the hot inactivated bacterial liquid of 40g staphylococcus aureus and surplus is made, and dry is that dregs of beans and the corn protein powder of 3.0:1 formed by mass ratio;
Three, the lactobacillus acidophilus seed culture fluid that respectively step 2 is obtained and Lactobacillus casei cheese subspecies the seed culture fluid by volume inoculum concentration of percentage composition 10% are inoculated in the fermentation medium, at 37 ° of C bottom fermentation 48h, obtain lactobacillus acidophilus tunning and Lactobacillus casei cheese subspecies tunning, wherein every liter of fermentation medium is made by mixed-powder and the distilled water of 500g, and mixed-powder is that bean cake powder, cotton dregs powder, dish dregs of rice powder and the corn flour of 13:3:3:4 formed by mass ratio;
Four, lactobacillus acidophilus tunning and Lactobacillus casei cheese subspecies tunning are dried to water content 14% respectively under 30 ° of C, are crushed to 40 orders then, 1:1 mixes tunning by mass ratio, obtains livestock and poultry with newborn source lactic acid bacteria antibacterial peptide additive.
The livestock and poultry that present embodiment obtains with newborn source lactic acid bacteria antibacterial peptide additive to the inhibition of 4 ° of former milk totals number of bacteria of C as shown in Figure 1, to the inhibition of 25 ° of former milk totals number of bacteria of C as shown in Figure 2.
It is slow than the increase of control group bacterial population with the experimental group of newborn source lactic acid bacteria antibacterial peptide additive to add livestock and poultry as shown in Figure 1, add back 0～24h livestock and poultry with newborn source lactic acid bacteria antibacterial peptide additive, the thalline number raises gradually in the former milk, illustrates that bacterium breeds in a large number in the former milk; After adding antibacterial peptide 24h, the thalline number descends gradually in the former milk, illustrate that antibacterial peptide brings into play bacteriostasis in former milk, makes the bacterium that exists in the former milk stop breeding, even dead, and the antibiotic flesh that Lactobacillus casei produces after adding 72h is controlled former milk bacterium number 15 * 10
4Below the cfu/ml, the former milk bacterium number of antibacterial peptide control that lactobacillus acidophilus produces is 30 * 10
4Below the cfu/ml, and control group total number of bacteria after 36h exceeds standard, and enters the growth platform phase, demonstrates good antibacterial peptide activity.
Under the condition of 25 ° of C, the former milk bacterium number of blank group increases very rapid as shown in Figure 2.It is slow relatively than the increase of control group bacterial population to add the antibacterial peptide group, and bacterial population begins to descend after 24h, produced the inhibitory or killing effect to bacterium, visible present embodiment livestock and poultry also can play obvious effect to milk preservation with newborn source lactic acid bacteria antibacterial peptide additive under 25 ° of C.
Claims (8)
1. livestock and poultry is characterized in that livestock and poultry follow these steps to realize with the production method of newborn source lactic acid bacteria antibacterial peptide additive with the production method of newborn source lactic acid bacteria antibacterial peptide additive:
One, frozen lactobacillus acidophilus bacterial classification and Lactobacillus casei cheese subspecies are activated with the MRS culture medium, then by volume the inoculum concentration of percentage composition 1%～5% with the lactobacillus acidophilus bacterial classification inoculation in proliferated culture medium A, at 35～40 ℃ of bottom fermentation 35～37h, obtain lactobacillus acidophilus propagation nutrient solution, by volume the inoculum concentration of percentage composition 1%～5% is inoculated in Lactobacillus casei cheese subspecies among the proliferated culture medium B, at 35～40 ℃ of bottom fermentation 35～37h, obtain Lactobacillus casei cheese subspecies propagation nutrient solution; Wherein every liter of proliferated culture medium A is made by the distilled water of 25～30g sucrose, 35～40g beef extract, 5～6g NaCl, 10～15g Tween-80 and surplus, and pH is 5.0; Every liter of proliferated culture medium B is made by the distilled water of 9～10g sucrose, 25～30g soy peptone, 6～7g NaCl, 9～10g Tween-80 and surplus, and pH is 6.0;
Two, the lactobacillus acidophilus propagation nutrient solution that step 1 is obtained and Lactobacillus casei cheese subspecies propagation nutrient solution respectively by volume the inoculum concentration of percentage composition 10%～20% be inoculated in the seed culture medium, at 35～40 ℃ of bottom fermentation 35～37h, obtain lactobacillus acidophilus seed culture fluid and Lactobacillus casei cheese subspecies seed culture fluid, wherein every liter of seed culture medium is by 200～250g dry, 15～20g urea, the distilled water of the hot inactivated bacterial liquid of 35～40g staphylococcus aureus and surplus is made, and dry is that dregs of beans and the corn protein powder of 2.8～3.0:1 formed by mass ratio;
Three, the lactobacillus acidophilus seed culture fluid that respectively step 2 is obtained and Lactobacillus casei cheese subspecies the seed culture fluid by volume inoculum concentration of percentage composition 5%～20% are inoculated in the fermentation medium, at 35～40 ℃ of bottom fermentation 45～50h, obtain lactobacillus acidophilus tunning and Lactobacillus casei cheese subspecies tunning, wherein every liter of fermentation medium is made by mixed-powder and the distilled water of 450～500g, and mixed-powder is that bean cake powder, cotton dregs powder, dish dregs of rice powder and the corn flour of 13:3:3:4 formed by mass ratio;
Four, lactobacillus acidophilus tunning and Lactobacillus casei cheese subspecies tunning are dried to water content 12%～15% respectively under 30～40 ℃, be crushed to 35～45 orders then, 1:1 mixes tunning by mass ratio, obtains livestock and poultry with newborn source lactic acid bacteria antibacterial peptide additive.
2. livestock and poultry according to claim 1 are with the production method of newborn source lactic acid bacteria antibacterial peptide additive, it is characterized in that step 1 by volume the inoculum concentration of percentage composition 5% with the lactobacillus acidophilus bacterial classification inoculation in proliferated culture medium A.
3. livestock and poultry according to claim 1 are with the production method of newborn source lactic acid bacteria antibacterial peptide additive, it is characterized in that every liter of proliferated culture medium A of step 1 is made by the distilled water of 30g sucrose, 40g beef extract, 5g NaCl, 15g Tween-80 and surplus, pH is 5.0.
4. livestock and poultry according to claim 1 are with the production method of newborn source lactic acid bacteria antibacterial peptide additive, it is characterized in that every liter of proliferated culture medium B of step 1 is made by the distilled water of 10g sucrose, 30g soy peptone, 7g NaCl, 10g Tween-80 and surplus, pH is 6.0.
5. livestock and poultry according to claim 1 are with the production method of newborn source lactic acid bacteria antibacterial peptide additive, it is characterized in that step 2 lactobacillus acidophilus propagation nutrient solution and Lactobacillus casei cheese subspecies propagation nutrient solution respectively by volume the inoculum concentration of percentage composition 10% be inoculated in the seed culture medium.
6. livestock and poultry according to claim 1 are with the production method of newborn source lactic acid bacteria antibacterial peptide additive, it is characterized in that every liter of seed culture medium of step 2 is by the 200g dry, 20g urea, the distilled water of the hot inactivated bacterial liquid of 40g staphylococcus aureus and surplus is made, and dry is that dregs of beans and the corn protein powder of 3.0:1 formed by mass ratio.
7. livestock and poultry according to claim 1 are with the production method of newborn source lactic acid bacteria antibacterial peptide additive, and the granularity that it is characterized in that step 2 dregs of beans and corn protein powder is 40 orders.
8. livestock and poultry according to claim 1 are with the production method of newborn source lactic acid bacteria antibacterial peptide additive, it is characterized in that step 3 lactobacillus acidophilus seed culture fluid and Lactobacillus casei cheese subspecies seed culture fluid by volume the inoculum concentration of percentage composition 10% be inoculated in the fermentation medium.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013101722833A CN103250867A (en) | 2013-05-10 | 2013-05-10 | Production method of raw milk lactic acid bacteria antimicrobial peptide additive for livestock and poultry |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013101722833A CN103250867A (en) | 2013-05-10 | 2013-05-10 | Production method of raw milk lactic acid bacteria antimicrobial peptide additive for livestock and poultry |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103250867A true CN103250867A (en) | 2013-08-21 |
Family
ID=48955379
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2013101722833A Pending CN103250867A (en) | 2013-05-10 | 2013-05-10 | Production method of raw milk lactic acid bacteria antimicrobial peptide additive for livestock and poultry |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103250867A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106391651A (en) * | 2016-08-31 | 2017-02-15 | 中创宏远（北京）环保科技有限公司 | Resource treatment method for animal carcass |
| CN112553131A (en) * | 2021-01-06 | 2021-03-26 | 广东辉阳生物科技有限公司 | Preparation method of anti-diarrhea compound microbial preparation |
| CN112625975A (en) * | 2021-01-06 | 2021-04-09 | 广东辉阳生物科技有限公司 | Preparation method of compound microbial preparation capable of being used together with antibiotics |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101886109A (en) * | 2010-06-25 | 2010-11-17 | 上海邦成生物科技有限公司 | Method for producing multifunctional antibacterial peptide |
| CN102210413A (en) * | 2011-06-27 | 2011-10-12 | 陕西大秦汉集团有限公司 | Dorking microorganism viable bacteria fermentation concentrated feed and production method thereof |
| WO2012110777A2 (en) * | 2011-02-18 | 2012-08-23 | Dupont Nutrition Biosciences Aps | Feed additive composition |
| CN102948621A (en) * | 2012-11-12 | 2013-03-06 | 东北农业大学 | Prebiotic peptide biological feed additive and preparation method and application thereof |
-
2013
- 2013-05-10 CN CN2013101722833A patent/CN103250867A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101886109A (en) * | 2010-06-25 | 2010-11-17 | 上海邦成生物科技有限公司 | Method for producing multifunctional antibacterial peptide |
| WO2012110777A2 (en) * | 2011-02-18 | 2012-08-23 | Dupont Nutrition Biosciences Aps | Feed additive composition |
| CN102210413A (en) * | 2011-06-27 | 2011-10-12 | 陕西大秦汉集团有限公司 | Dorking microorganism viable bacteria fermentation concentrated feed and production method thereof |
| CN102948621A (en) * | 2012-11-12 | 2013-03-06 | 东北农业大学 | Prebiotic peptide biological feed additive and preparation method and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 乔彬，等: "产抗菌肽乳源乳酸菌的筛选及定向培养", 《中国乳品工业》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106391651A (en) * | 2016-08-31 | 2017-02-15 | 中创宏远（北京）环保科技有限公司 | Resource treatment method for animal carcass |
| CN112553131A (en) * | 2021-01-06 | 2021-03-26 | 广东辉阳生物科技有限公司 | Preparation method of anti-diarrhea compound microbial preparation |
| CN112625975A (en) * | 2021-01-06 | 2021-04-09 | 广东辉阳生物科技有限公司 | Preparation method of compound microbial preparation capable of being used together with antibiotics |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104195075B (en) | Enterococcus faecium EF08 as well as feed additive and feed containing enterococcus faecium EF08 | |
| CN102168045B (en) | Bacillus subtilis preparation and preparation method thereof | |
| CN102630852B (en) | Fermented broiler feed and preparation method thereof | |
| CN103039699B (en) | Mixed fermentation feed and preparation method and application method of mixed fermentation feed | |
| CN102742728B (en) | Livestock and poultry composite probiotics feed additive and premix, concentrate feed and batch | |
| CN106906154B (en) | Lactobacillus plantarum strain, feed additive and feed thereof | |
| CN102907563B (en) | Method for preparing high-activity probiotic preparation for livestock breeding | |
| CN102356828B (en) | Piglet early-stage microbial fermented antibiotic-free feed | |
| CN101278702B (en) | Novel microbial feed additive and method of producing the same | |
| CN102696884B (en) | Composite feed additive product | |
| CN105851492A (en) | Traditional Chinese medicine probiotic fermented feed and preparation method | |
| CN102422996B (en) | Preliminary microbial fermentation antibiotic-free feed for growth fattening pigs | |
| CN105475624A (en) | Microbial fermentation feed as well as production method and application thereof | |
| CN106962609A (en) | A kind of direct putting type biological fermentation feed additive and its production and use | |
| CN103907749A (en) | Biological fermentation active carrier type composite premix feed as well as preparation method and application thereof | |
| CN105994941B (en) | A kind of nonreactive feed of microbial fermentation preparation | |
| CN106591182A (en) | Microecological preparation with bacillus subtilis fermenting astragalus membranaceus and application thereof | |
| CN102028110A (en) | Probiotic-astragalus polysaccharide composition for feeding commercial chicken | |
| CN106901015A (en) | Feed addictive containing active probiotic and its production and use | |
| CN102669435A (en) | Feed additive product | |
| CN106359904A (en) | Application of lactobacillus reuteri in replacement of feed antibiotic | |
| CN104212735A (en) | Poultry composite probiotic preparation and preparation method thereof | |
| CN102919549A (en) | Method for preparing aquatic feed additive by solid state fermentation of peanut meal | |
| CN103555707A (en) | Method for preparing compound bacterium agent | |
| CN110179015A (en) | A kind of high-survival rate fermented shrimp material and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20130821 |