CN103240137B - Quantitative collection box of little arthropod soil animals - Google Patents
Quantitative collection box of little arthropod soil animals Download PDFInfo
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- CN103240137B CN103240137B CN201310150590.1A CN201310150590A CN103240137B CN 103240137 B CN103240137 B CN 103240137B CN 201310150590 A CN201310150590 A CN 201310150590A CN 103240137 B CN103240137 B CN 103240137B
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Abstract
The invention provides a quantitative collection box of little arthropod soil animals. The quantitative collection box comprises a box body and a separation and collection device, wherein the separation and collection device is arranged in the box body; a heating device is arranged on a top cover of the box body; a refrigerating device is arranged at the bottom of the box body; the separation and collection device comprises a sample holder, a bearing plate and one or more separator-collectors; the bearing plate is arranged on the sample holder and is provided with bearing holes; each separator-collector is arranged in the corresponding bearing hole and comprises a sample cup, a sieving net, a net hoop and a collection vessel; and each sieving net is arranged at the lower end of the corresponding sample cup and then is connected with the corresponding collection vessel after being fixed by the corresponding net hoop. The quantitative collection box disclosed by the invention can be applied to separation of the little arthropod soil animals and can accurately quantify the little arthropod soil animals in a soil sample. Moreover, the quantitative collection box provides a lucifugal environment so as to prevent easily-photolyzed substances in soil from being decomposed, and thus the actual condition of the soil can be really reflected.
Description
Technical field
The present invention relates to eco-environment technology field, a little segmental appendage class soil animal quantitative collection case, effectively can be separated little segmental appendage class soil animal specifically.
Background technology
The risk assessment of contaminated soil is the key link of carrying out contaminated soil risk management and reparation.Soil animal is the ecological receptor needing when carrying out soil pollution urban eco landscape forest to consider.Wherein, little segmental appendage class soil animal such as springtail, acarid etc. is the ecological receptor that current soil ecology risk assessment and the timing of soil ecology reference system are commonly used.
For being separated little segmental appendage class soil animal from soil, 20 beginning of the century Italy scholar Berlesc have invented baking worm funnel, by the improvement of Tullgren, be used widely, people are called Tullgren method or dry funnel method, its principle mainly utilizes the motion of soil animal self and the characteristic of lucifuge, and the animal in soil is expelled out.Be mainly used in the separation and Extraction of the Meso-micro soil fauna of the long 0.2-2 mm of body.Current Chinese scholars still adopts Tullgren method to be separated to the collection of Meso-micro soil fauna more.But common equipment is comparatively simple and crude, cannot carry out temperature control, collection efficiency is low and can only carry out qualitative or sxemiquantitative and collect, and cannot meet the accurate quantitative analysis demand needed for soil fauna effect study.On the other hand, original equipment is used for natural soils investigation, and the illumination methods that uses heats more, easily has an impact to such as the organic pollution etc. of the pollutant in soil.
Summary of the invention
the technical problem solved:the object of this invention is to provide a kind of little segmental appendage class soil animal quantitative collection case, can be applicable to the separation of little segmental appendage class soil animal, and accurate quantitative analysis is carried out to the little segmental appendage class soil animal in pedotheque.In addition, the device provides the environment of a lucifuge, avoid the decomposition of easy photodissociation material in soil, the actual state of true reflect soil.
technical scheme:little segmental appendage class soil animal quantitative collection case, comprise casing and separated and collected device, separated and collected device is located in casing; Described top cover of box is provided with heater, and bottom half is provided with refrigerating plant; Described separated and collected device comprises specimen holder, loading plate and separated and collected device, and loading plate is located on specimen holder, and it offers bearing holes, and separated and collected device is located in bearing holes; Described separated and collected device comprises specimen cup, Screening Network, net hoop and collects ware, and Screening Network is located at specimen cup lower end, is connected after fixing by net hoop with collection ware.
Described loading plate is that porous material is made.
Described loading plate is provided with thermal insulation layer, and described thermal insulation layer is made up of heat-barrier material.
Described cabinet wall is stainless steel material, and outer wall is exotic material.
The aperture of described Screening Network is 1mm.
Casing is provided with programmed temperature-rising controller: XMT-8000P programmable, intelligent temperature controller, and described heater is electrothermal tube, and this electrothermal tube is connected with programmed temperature-rising controller.
Casing is provided with programmed cooling controller simultaneously: XMT-8000P programmable, intelligent temperature controller, described refrigerating plant is refrigerator pipes, and this refrigerator pipes is connected with programmed cooling controller.
Described loading plate or thermal insulation layer outer and cabinet wall contact portion gapless, separated and collected device and bearing holes contact portion gapless.
Described bearing holes is provided with the cover plate of flexible connection.
Gypsum/active carbon Mixed culture matrix or ethylene glycol is covered with bottom described collection ware.
beneficial effect:the present invention is simple to operation, make the upper and lower formation temperature of separated and collected device poor by loading plate, formation temperature gradient difference in casing can be made further by arranging thermal insulation layer, this device is provided with temperature programming and can makes temperature precise control with cooling controller simultaneously, avoid the too high death of animal Yin Wendu in soil, temperature is too low numb with cold, and encapsulation process has been carried out to contact point in casing, ensure that cold and hot transmission major part is by separated and collected device, reduce the convection current of air, ensure that the quantitative collection of little segmental appendage class soil animal, react the truth of pedotheque.
Accompanying drawing explanation
Fig. 1 is the body structure schematic diagram of little segmental appendage class soil animal quantitative collection case, casing 1, heater 3, refrigerating plant 4 in figure;
Fig. 2 is the separated and collected apparatus structure schematic diagram of little segmental appendage class soil animal quantitative collection case, separated and collected device 2, specimen holder 5, loading plate 6, separated and collected device 7, bearing holes 8, specimen cup 9, Screening Network 10, net hoop 11, collection ware 12, thermal insulation layer 13 in figure.
Detailed description of the invention
Following detailed description of the invention does not limit technical scheme of the present invention in any form, and the technical scheme that the mode that every employing is equal to replacement or equivalent transformation obtains all drops on protection scope of the present invention.
embodiment 1:
Little segmental appendage class soil animal quantitative collection case, comprise casing 1 and separated and collected device 2, separated and collected device is located in casing, and cabinet wall is stainless steel material, and outer wall is exotic material; Described top cover of box is provided with heater 3, and bottom half is provided with refrigerating plant 4; Described separated and collected device comprises specimen holder 5, loading plate 6 and separated and collected device 7, loading plate is located on specimen holder, loading plate is provided with thermal insulation layer 13, and described thermal insulation layer is made up of heat-barrier material, such as foamed plastics or sponge, described loading plate is that porous heat-resistant plastics or metal are made, it offers bearing holes 8, separated and collected device is located in bearing holes, and bearing holes 8 is provided with the cover plate of flexible connection, if part bearing holes does not place separated and collected device in use, hole is blocked by available cover plate; Described separated and collected device comprises specimen cup 9, Screening Network 10, net hoop 11 and collects ware 12, Screening Network is located at specimen cup lower end, be connected with collection ware after fixing by net hoop, the aperture of Screening Network is 1mm, casing is provided with programmed temperature-rising controller: XMT-8000P programmable, intelligent temperature controller, described heater is electrothermal tube, this electrothermal tube is connected with programmed temperature-rising controller, casing is provided with programmed cooling controller simultaneously: XMT-8000P programmable, intelligent temperature controller, described refrigerating plant is refrigerator pipes, and this refrigerator pipes is connected with programmed cooling controller.Loading plate or thermal insulation layer outer and cabinet wall contact portion gapless, separated and collected device and bearing holes contact portion gapless, to guarantee that in casing, air does not want convection current, form the temperature difference of upper heat and lower cold.
embodiment 2:
Adopt the device described in embodiment 1, when collecting ecological effect experiment soil little segmental appendage class animal sample, nylon mesh subnetting 10 is fixed on the bottom of specimen cup 9 by net hoop 11, pedotheque is loaded in specimen cup, the collection ware 12 that bottom is covered with gypsum/active carbon Mixed culture matrix is connected to below net hoop 11, be placed on specimen cup loading plate 6, be covered with thermal insulation layer 13.Open equipment total power switch, heat switch, freeze switch.Regulate programmed cooling controller, temperature is set to 5 DEG C.Regulate programmed temperature-rising controller, collect 12h for 25 DEG C, collect 12h for 35 DEG C, collect 24h for 45 DEG C, amount to 48h.Collection efficiency reaches more than 95%.
embodiment 3:
Adopt the device described in embodiment 1, when collecting natural soils investigation soil little segmental appendage class animal sample, nylon mesh subnetting 10 is fixed on the bottom of specimen cup 9 by net hoop 11, pedotheque is loaded in specimen cup, the collection ware 12 filling ethylene glycol 20mL is connected to below net hoop 11, be placed on specimen cup loading plate 6, be covered with thermal insulation layer 13.Open equipment total power switch, heat switch, freeze switch.Regulate programmed cooling controller, temperature is set to 5 DEG C.Regulate programmed temperature-rising controller, from 25 DEG C, every 24h heats up 5 DEG C, collects sample 5 days.Collection efficiency reaches more than 90%.
Claims (1)
1. little segmental appendage class soil animal quantitative collection case, it is characterized in that comprising casing (1) and separated and collected device (2), separated and collected device is located in casing, and cabinet wall is stainless steel material, and outer wall is exotic material; Described top cover of box is provided with heater (3), and bottom half is provided with refrigerating plant (4); Described separated and collected device comprises specimen holder (5), loading plate (6) and separated and collected device (7), loading plate is located on specimen holder, loading plate is provided with thermal insulation layer (13), described thermal insulation layer is made up of heat-barrier material, described loading plate is that porous heat-resistant plastics or metal are made, it offers bearing holes (8), separated and collected device is located in bearing holes, bearing holes (8) is provided with the cover plate of flexible connection, if part bearing holes does not place separated and collected device in use, hole is blocked by available cover plate; Described separated and collected device comprises specimen cup (9), Screening Network (10), net hoop (11) and collects ware (12), Screening Network is located at specimen cup lower end, be connected with collection ware after fixing by net hoop, the aperture of Screening Network is 1mm, casing is provided with programmed temperature-rising controller XMT-8000P programmable, intelligent temperature controller, described heater is electrothermal tube, this electrothermal tube is connected with programmed temperature-rising controller, casing is provided with simultaneously programmed cooling controller XMT-8000P programmable, intelligent temperature controller, described refrigerating plant is refrigerator pipes, and this refrigerator pipes is connected with programmed cooling controller; Loading plate or thermal insulation layer outer and cabinet wall contact portion gapless, separated and collected device and bearing holes contact portion gapless, to guarantee that in casing, air does not want convection current, form the temperature difference of upper heat and lower cold.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310150590.1A CN103240137B (en) | 2013-04-27 | 2013-04-27 | Quantitative collection box of little arthropod soil animals |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310150590.1A CN103240137B (en) | 2013-04-27 | 2013-04-27 | Quantitative collection box of little arthropod soil animals |
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| Publication Number | Publication Date |
|---|---|
| CN103240137A CN103240137A (en) | 2013-08-14 |
| CN103240137B true CN103240137B (en) | 2015-07-01 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201310150590.1A Expired - Fee Related CN103240137B (en) | 2013-04-27 | 2013-04-27 | Quantitative collection box of little arthropod soil animals |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104998707B (en) * | 2015-07-17 | 2017-04-19 | 河北爱能生物科技股份有限公司 | Unified liquid adding and changing oscillation shaking box applied in decellularization reaction process |
| CN106670088A (en) * | 2017-02-14 | 2017-05-17 | 中国科学院华南植物园 | Separation device for small animals in soil |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2762102Y (en) * | 2005-01-05 | 2006-03-01 | 中国科学院东北地理与农业生态研究所 | Soil animal separator |
| CN201048523Y (en) * | 2006-11-27 | 2008-04-23 | 新疆维吾尔自治区疾病预防控制中心 | Multifunctional small animal separator |
| KR20110039595A (en) * | 2009-10-12 | 2011-04-20 | 서울대학교산학협력단 | Pitfall trap with filter module |
| CN102407172A (en) * | 2011-09-07 | 2012-04-11 | 中国科学院南京土壤研究所 | Portable soil animal collection device |
| CN202823413U (en) * | 2012-08-17 | 2013-03-27 | 乐山师范学院 | Portable soil animal collecting device |
| CN203227508U (en) * | 2013-04-27 | 2013-10-09 | 中国科学院南京土壤研究所 | Small-section limb-type soil animal separation and quantitative collection device |
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