CN101398348A - Sampling apparatus for plant and nematode and detection method thereof - Google Patents
Sampling apparatus for plant and nematode and detection method thereof Download PDFInfo
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- CN101398348A CN101398348A CNA2008100720632A CN200810072063A CN101398348A CN 101398348 A CN101398348 A CN 101398348A CN A2008100720632 A CNA2008100720632 A CN A2008100720632A CN 200810072063 A CN200810072063 A CN 200810072063A CN 101398348 A CN101398348 A CN 101398348A
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Abstract
The invention provides a sampling device used for plant nematode and soil nematode and a detection method thereof, relating to the plant nematode and the soil nematode. The sampling device is provided with a box body, an atomizer and at least one set of separation device; the box body is provided with a movable box cover; the position in the box body close to the bottom is provided with a grid-typed cushion plate; one side of the box body close to the bottom is provided with an drainage valve; the atomizer is arranged at the center of the box body; the separation device is provided with a screen, a screen bracket and nematode receiving utensils; the screen is sheathed on the screen bracket and arranged above the nematode receiving utensils; and the separation device is put on the grid-typed cushion plate. Plant root samples or soil samples are put in the screen; the box cover is opened, the screen bracket is sheathed on the nematode receiving utensil, subsequently, the screen with embedded samples is arranged on the screen bracket; the separation device in the sampling device of plant nematode and the soil nematode is uniformly put in the box body and the box cover is covered; the power of the atomizer is turned on and the atomizer generates water mist; after 12-24 hours, the power is turned off, the box cover is opened and the nematode receiving utensils are sequentially taken out and put under a binocular dissecting microscope and an advanced microscope so as to check the nematode and the types of the nematode.
Description
Technical field
The present invention relates to Plant nematode and soil nematodes, especially relate to sampling apparatus and the detection method thereof of a kind of Plant nematode and soil nematodes.
Background technology
Nematodiasis is the class disease of many plants, and it makes crop failure 30%～50%, causes the tremendous economic loss.Because most nematodes are present in plant root, therefore often out in the cold, mistaken diagnosis takes place through regular meeting, not only cause unnecessary economic loss and disease popular, even pesticide abuse, pollute crops, contaminated environment.So from plant tissue and soil, isolate nematode, identify that its kind just seems very necessary to carry out correct diagnosis.
Usually detecting nematode all is to continue to use the graceful funnel method of Bel, and its basic device is the funnel of a suitable diameter, and one section proofed sleeve of filler tube termination is with the switching of spring water stopper control tube.Funnel is placed on the annulus of brandreth, fills with clear water in it.Get radicula from plant root, wrap in the square gauze after cleaning, be immersed in the water.Nematode is swum out of in root, sinks at the bottom of filler tube through gauze.Behind diel, open spring clamp, flow out low amounts of water, be collected in the glass dish, water contains nematode.Find nematode with bitubular anatomical lens, again the nematode that contains with microscopic examination water be any nematode (Bi Zhishu, lijin compiles. plant nematology, agriculture publishing house, 211-212).Though this method can be isolated nematode, have following significant disadvantages: it much is dead separating 1) that the nematode obtain has; 2) temperature high season, water is smelly through regular meeting; 3) funnel can only separate a duplicate samples, inefficiency, and a plurality of funnels tie up lab space, and water wasting.
Summary of the invention
Purpose of the present invention is intended to provide a kind of high efficiency, can detects the plant of nematode live body and sampling apparatus and the detection method thereof of soil nematodes fast from plant or soil.
The sampling apparatus of plant of the present invention and soil nematodes is provided with the tripping device of casing, atomizer and at least 1 cover, and casing is provided with the active box cover, is provided with the grid type backing plate by the bottom in the casing, and a water discharging valve is established in the nearly bottom of casing one side; Atomizer is located at casing central authorities; Tripping device is provided with screen cloth, screen cloth support and connects the worm ware, and screen cloth is enclosed within on the screen cloth support and places and connects worm ware top, and tripping device is placed on the grid type backing plate.
Screen cloth is preferably 40～200 purpose sieves.
On the sampling apparatus of plant of the present invention and soil nematodes, preferably dispose a temperature regulating device, can in 20～45 ℃ of scopes, steady temperature be set, be suitable for day use in cold area.
The detection method of plant of the present invention and soil nematodes may further comprise the steps:
1) plant root sample or pedotheque are put into screen cloth;
2) open the casing cover, screen frame (unit) is enclosed within connects on the worm ware, again the screen cloth of built-in sample is propped up on screen frame (unit);
3) tripping device in the sampling apparatus of plant and soil nematodes is placed in the casing equably, covers the case cover;
4) connect the atomizer power supply, atomizer produces water smoke;
5) behind 12～24h, powered-down is opened the case cover, takes out one by one and connects the worm ware, is placed on the kind of checking nematode and nematode under bitubular anatomical lens and the high-power microscope.
The principle that the present invention utilizes nematode to move on moisture film, the very thin moisture film that the water smoke that produces by atomizer forms at sample surfaces, thereby making Plant nematode and soil nematodes dissociate out from sample is identified, it has following advantage: 1) water smoke that constantly produces provides sufficient oxygen, and the isolated nematode overwhelming majority is (nematode that separates with the tray method of funnel method have I haven't seen you for ages dies) that lives; 2) avoid the water sample that contains nematode of resulting separation smelly; 3) using water wisely also reduces taking lab space; 4) directly get and connect the worm ware and get final product microscopy, easy to operate; 5) can separate multiple sample, high efficiency simultaneously.
Description of drawings
Fig. 1 is the structural representation of the sampling apparatus embodiment 1 of plant of the present invention and soil nematodes.
Fig. 2 is the plan structure synoptic diagram of the sampling apparatus embodiment 1 of plant of the present invention and soil nematodes.
Embodiment
Below by embodiment the present invention is elaborated.
As depicted in figs. 1 and 2,24 cover tripping devices 3 are arranged in around the atomizer 2 in the casing 1 of 100cm * 100cm * 50cm equably.Every cover tripping device comprises screen cloth 31, screen frame (unit) 32 and connects worm ware 33.Screen cloth 31 is 80 purpose sieves, and it is transparent connecing worm ware 33, and diameter 9cm is propping up screen frame (unit) 32 in it, screen frame (unit) 32 screen cloth 31 of putting on the shelf.Casing 1 adopts organic glass to make, and inside can be seen clearly.Establish a grid type backing plate 12 apart from bottom 5cm place in the case, the unnecessary aggegation water in atomizing back flows under the grid, and a valve 13 finishes the back draining for experiment usefulness is established in the nearly bottom of casing one side.Also be provided with temperature regulating device 4 in the case.
Gather plant root sample, be cut into 1～2cm segment after cleaning and put into screen cloth.Open case cover 11, screen cloth is propped up with screen frame (unit) connecing on the worm ware.Connect the atomizer power supply, the water smoke that atomizer produces is condensed into moisture film at sample surfaces, and nematode dissociates out in root, moisture film thicken the water droplet that forms nematode lentamente drop go into connecing in the worm ware of its below.Behind the 12h, powered-down is opened case lid, takes out to connect the worm ware supernatant that inclines, and is placed on the kind of inspection nematode and nematode bitubular anatomical lens and high-power microscope under.
The earth sample that fetches earth is put into 200 purpose screen clothes, and the separation method of nematode is with embodiment 1, and behind the 24h, powered-down is opened the case cover, takes out to connect the worm ware supernatant that inclines, and is placed on the kind of inspection nematode and nematode bitubular anatomical lens and high-power microscope under.
Claims (5)
1. the sampling apparatus of plant and soil nematodes is characterized in that being provided with casing, atomizer and at least 1 tripping device that overlaps, and casing is provided with the active box cover, is provided with the grid type backing plate by the bottom in the casing, and a water discharging valve is established in the nearly bottom of casing one side; Atomizer is located at casing central authorities; Tripping device is provided with screen cloth, screen cloth support and connects the worm ware, and screen cloth is enclosed within on the screen cloth support and places and connects worm ware top, and tripping device is placed on the grid type backing plate.
2. the sampling apparatus of plant as claimed in claim 1 and soil nematodes is characterized in that screen cloth is 40～200 purpose sieves.
3. the sampling apparatus of plant as claimed in claim 1 and soil nematodes is characterized in that being provided with a temperature regulating device.
4. the sampling apparatus of plant as claimed in claim 3 and soil nematodes, the temperature that it is characterized in that temperature regulating device is 20～45 ℃.
5. the detection method of plant and soil nematodes is characterized in that may further comprise the steps:
1) plant root sample or pedotheque are put into screen cloth;
2) open the casing cover, screen frame (unit) is enclosed within connects on the worm ware, again the screen cloth of built-in sample is propped up on screen frame (unit);
3) tripping device in the sampling apparatus of plant and soil nematodes is placed in the casing equably, covers the case cover;
4) connect the atomizer power supply, atomizer produces water smoke;
5) behind 12～24h, powered-down is opened the case cover, takes out one by one and connects the worm ware, is placed on the kind of checking nematode and nematode under bitubular anatomical lens and the high-power microscope.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008100720632A CN101398348B (en) | 2008-11-03 | 2008-11-03 | Sampling apparatus for plant and nematode and detection method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008100720632A CN101398348B (en) | 2008-11-03 | 2008-11-03 | Sampling apparatus for plant and nematode and detection method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101398348A true CN101398348A (en) | 2009-04-01 |
| CN101398348B CN101398348B (en) | 2010-08-11 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2008100720632A Expired - Fee Related CN101398348B (en) | 2008-11-03 | 2008-11-03 | Sampling apparatus for plant and nematode and detection method thereof |
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| Country | Link |
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| CN (1) | CN101398348B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102033006A (en) * | 2010-09-20 | 2011-04-27 | 杜宇 | Intelligent nematode separating box |
| CN102357423A (en) * | 2011-09-09 | 2012-02-22 | 中国科学院东北地理与农业生态研究所 | Potassium chloride flotation method of plant and soil nematodes |
| CN102407172A (en) * | 2011-09-07 | 2012-04-11 | 中国科学院南京土壤研究所 | Portable soil animal collection device |
-
2008
- 2008-11-03 CN CN2008100720632A patent/CN101398348B/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102033006A (en) * | 2010-09-20 | 2011-04-27 | 杜宇 | Intelligent nematode separating box |
| CN102033006B (en) * | 2010-09-20 | 2012-02-29 | 杜宇 | Intelligent nematode separating box |
| CN102407172A (en) * | 2011-09-07 | 2012-04-11 | 中国科学院南京土壤研究所 | Portable soil animal collection device |
| CN102357423A (en) * | 2011-09-09 | 2012-02-22 | 中国科学院东北地理与农业生态研究所 | Potassium chloride flotation method of plant and soil nematodes |
| CN102357423B (en) * | 2011-09-09 | 2013-04-10 | 中国科学院东北地理与农业生态研究所 | Potassium chloride flotation method of plant and soil nematodes |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101398348B (en) | 2010-08-11 |
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Granted publication date: 20100811 Termination date: 20171103 |