CN103240137A - Quantitative collection box of little arthropod soil animals - Google Patents
Quantitative collection box of little arthropod soil animals Download PDFInfo
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- CN103240137A CN103240137A CN2013101505901A CN201310150590A CN103240137A CN 103240137 A CN103240137 A CN 103240137A CN 2013101505901 A CN2013101505901 A CN 2013101505901A CN 201310150590 A CN201310150590 A CN 201310150590A CN 103240137 A CN103240137 A CN 103240137A
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Abstract
The invention provides a quantitative collection box of little arthropod soil animals. The quantitative collection box comprises a box body and a separation and collection device, wherein the separation and collection device is arranged in the box body; a heating device is arranged on a top cover of the box body; a refrigerating device is arranged at the bottom of the box body; the separation and collection device comprises a sample holder, a bearing plate and one or more separator-collectors; the bearing plate is arranged on the sample holder and is provided with bearing holes; each separator-collector is arranged in the corresponding bearing hole and comprises a sample cup, a sieving net, a net hoop and a collection vessel; and each sieving net is arranged at the lower end of the corresponding sample cup and then is connected with the corresponding collection vessel after being fixed by the corresponding net hoop. The quantitative collection box disclosed by the invention can be applied to separation of the little arthropod soil animals and can accurately quantify the little arthropod soil animals in a soil sample. Moreover, the quantitative collection box provides a lucifugal environment so as to prevent easily-photolyzed substances in soil from being decomposed, and thus the actual condition of the soil can be really reflected.
Description
Technical field
The present invention relates to the ecological environment technical field, a trifle limb class soil animal quantitative collection case can effectively separate trifle limb class soil animal specifically.
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Background technology
The risk assessment of contaminated soil is the key link of carrying out contaminated soil risk management and reparation.Soil animal is the ecological acceptor that need consider when carrying out the assessment of soil pollution ecological risk.Wherein, trifle limb class soil animal such as springtail, acarid etc. are the ecological acceptors that present soil ecology risk assessment and soil ecology reference system are regularly used always.
For from soil, separating trifle limb class soil animal, 20 beginnings of the century, Italy scholar Berlesc invented baking worm funnel, improvement by Tullgren, be used widely, people are called Tullgren method or dried funnel method, its principle mainly is to utilize the motion of soil animal self and the characteristic of lucifuge, and the animal in the soil is expelled out.Be mainly used in the separation and Extraction of the middle-size and small-size soil animal of the long 0.2-2 mm of body.Chinese scholars still adopts the Tullgren methods to separate to the collection of middle-size and small-size soil animal more at present.But common equipment is comparatively simple and crude, can't carry out temperature control, and collection efficiency is low and can only carry out qualitative or the sxemiquantitative collection, can't satisfy the required accurately quantitative demand of soil animal Research on Ecological Effect.On the other hand, original equipment is used for the natural soils investigation, uses the illumination mode to heat more, easily the pollutant in the soil such as organic pollution etc. is exerted an influence.
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Summary of the invention
The technical problem that solves:The purpose of this invention is to provide a kind of trifle limb class soil animal quantitative collection case, can be applicable to the separation of trifle limb class soil animal, and the trifle limb class soil animal in the pedotheque is carried out accurately quantitatively.In addition, this device provides the environment of a lucifuge, avoids the easily decomposition of photodissociation material in the soil, truly reflects the actual state of soil.
Technical scheme:Trifle limb class soil animal quantitative collection case comprises casing and separated and collected device, and the separated and collected device is located in the casing; Described casing top cover is provided with heater, and bottom half is provided with refrigerating plant; Described separated and collected device comprises specimen holder, loading plate and separated and collected device, and loading plate is located on the specimen holder, offers bearing holes on it, and the separated and collected device is located in the bearing holes; Described separated and collected device comprises specimen cup, Screening Network, net hoop and collection ware, and Screening Network is located at the specimen cup lower end, is connected with the collection ware by the fixing back of net hoop.
Described loading plate is that porous material is made.
Described loading plate is provided with thermal insulation layer, and described thermal insulation layer is made by heat-barrier material.
Described cabinet wall is stainless steel material, and outer wall is exotic material.
The aperture of described Screening Network is 1mm.
Casing is provided with programmed temperature-rising controller: XMT-8000P programmable, intelligent temperature controller, described heater are electrothermal tube, and this electrothermal tube is connected with programmed temperature-rising controller.
Be provided with the programmed cooling controller on the casing simultaneously: XMT-8000P programmable, intelligent temperature controller, described refrigerating plant are refrigerator pipes, and this refrigerator pipes is connected with the programmed cooling controller.
Described loading plate or thermal insulation layer outer and cabinet wall contact portion do not have the gap, and separated and collected device and bearing holes contact portion do not have the gap.
Described bearing holes is provided with the cover plate of flexible connection.
Described collection ware bottom is covered with gypsum/active carbon Mixed culture matrix or ethylene glycol.
Beneficial effect:The present invention is simple to operation, up and down formation temperature is poor to make the separated and collected device by loading plate, further can make formation temperature gradient difference in the casing by thermal insulation layer is set, simultaneously this device be provided with temperature programming and the cooling controller can be so that the temperature precise control, avoid the too high death of animal Yin Wendu in the soil, temperature is crossed low numb with cold, and contact point in the casing has been carried out encapsulation process, guarantee that cold and hot transmission major part is by the separated and collected device, reduce the convection current of cold and hot air, guarantee the quantitative collection of trifle limb class soil animal, reacted the truth of pedotheque.
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Description of drawings
Fig. 1 is the body structure schematic diagram of trifle limb class soil animal quantitative collection case, casing 1, heater 3, refrigerating plant 4 among the figure;
Fig. 2 is the separated and collected apparatus structure schematic diagram of trifle limb class soil animal quantitative collection case, separated and collected device 2, specimen holder 5, loading plate 6, separated and collected device 7, bearing holes 8, specimen cup 9, Screening Network 10, net hoop 11, collection ware 12, thermal insulation layer 13 among the figure.
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The specific embodiment
The following specific embodiment does not limit technical scheme of the present invention in any form, and every employing is equal to replaces or technical scheme that the mode of equivalent transformation obtains all drops on protection scope of the present invention.
Embodiment 1:
Trifle limb class soil animal quantitative collection case comprises casing 1 and separated and collected device 2, and the separated and collected device is located in the casing, and cabinet wall is stainless steel material, and outer wall is exotic material; Described casing top cover is provided with heater 3, and bottom half is provided with refrigerating plant 4; Described separated and collected device comprises specimen holder 5, loading plate 6 and separated and collected device 7, loading plate is located on the specimen holder, loading plate is provided with thermal insulation layer 13, and described thermal insulation layer is made by heat-barrier material, for example foamed plastics or sponge, described loading plate is that porous heat-resistant plastics or metal are made, offer bearing holes 8 on it, the separated and collected device is located in the bearing holes, and bearing holes 8 is provided with the cover plate of flexible connection, if the part bearing holes is not placed the separated and collected device, available cover plate is blocked the hole in use; Described separated and collected device comprises specimen cup 9, Screening Network 10, net hoop 11 and collects ware 12, Screening Network is located at the specimen cup lower end, be connected with the collection ware by the fixing back of net hoop, the aperture of Screening Network is 1mm, casing is provided with programmed temperature-rising controller: XMT-8000P programmable, intelligent temperature controller, described heater is electrothermal tube, this electrothermal tube is connected with programmed temperature-rising controller, be provided with the programmed cooling controller on the casing simultaneously: XMT-8000P programmable, intelligent temperature controller, described refrigerating plant is refrigerator pipes, and this refrigerator pipes is connected with the programmed cooling controller.Loading plate or thermal insulation layer outer and cabinet wall contact portion do not have the gap, and separated and collected device and bearing holes contact portion do not have the gap, to guarantee that cold and hot air is not wanted convection current in the casing, form the temperature difference of upper heat and lower cold.
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Embodiment 2:
The device that adopts embodiment 1 to put down in writing, when collecting ecological effect experiment soil trifle limb class animal sample, net hoop 11 is fixed in nylon mesh subnetting 10 bottom of specimen cup 9, pedotheque is packed in the specimen cup, the collection ware 12 that the bottom is covered with gypsum/active carbon Mixed culture matrix is connected to net hoop 11 belows, place on the specimen cup loading plate 6, be covered with thermal insulation layer 13.Open the equipment total power switch, heat switch, the refrigeration switch.Regulate the programmed cooling controller, temperature is set to 5 ℃.Regulate programmed temperature-rising controller, collect 12h for 25 ℃, collect 12h for 35 ℃, collect 24h for 45 ℃, amount to 48h.Collection efficiency reaches more than 95%.
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Embodiment 3:
The device that adopts embodiment 1 to put down in writing, when collecting natural soils investigation soil trifle limb class animal sample, net hoop 11 is fixed in nylon mesh subnetting 10 bottom of specimen cup 9, pedotheque is packed in the specimen cup, the collection ware 12 that fills ethylene glycol 20mL is connected to net hoop 11 belows, place on the specimen cup loading plate 6, be covered with thermal insulation layer 13.Open the equipment total power switch, heat switch, the refrigeration switch.Regulate the programmed cooling controller, temperature is set to 5 ℃.Regulate programmed temperature-rising controller, from 25 ℃, every 24h heats up 5 ℃, collects sample 5 days.Collection efficiency reaches more than 90%.
Claims (10)
1. trifle limb class soil animal quantitative collection case is characterized in that comprising casing (1) and separated and collected device (2), and the separated and collected device is located in the casing; Described casing top cover is provided with heater (3), and bottom half is provided with refrigerating plant (4); Described separated and collected device comprises specimen holder (5), loading plate (6) and separated and collected device (7), and loading plate is located on the specimen holder, offers bearing holes (8) on it, and the separated and collected device is located in the bearing holes; Described separated and collected device comprises specimen cup (9), Screening Network (10), net hoop (11) and collection ware (12), and Screening Network is located at the specimen cup lower end, is connected with the collection ware by the fixing back of net hoop.
2. trifle limb class soil animal quantitative collection case according to claim 1 is characterized in that described loading plate is that porous material is made.
3. trifle limb class soil animal quantitative collection case according to claim 1 is characterized in that described loading plate is provided with thermal insulation layer (13), and described thermal insulation layer is made by heat-barrier material.
4. trifle limb class soil animal quantitative collection case according to claim 1 is characterized in that described cabinet wall is stainless steel material, and outer wall is exotic material.
5. trifle limb class soil animal quantitative collection case according to claim 1, the aperture that it is characterized in that described Screening Network is 1mm.
6. trifle limb class soil animal quantitative collection case according to claim 1, it is characterized in that casing is provided with programmed temperature-rising controller: XMT-8000P programmable, intelligent temperature controller, described heater is electrothermal tube, and this electrothermal tube is connected with programmed temperature-rising controller.
7. trifle limb class soil animal quantitative collection case according to claim 1, it is characterized in that casing is provided with the programmed cooling controller: XMT-8000P programmable, intelligent temperature controller, described refrigerating plant is refrigerator pipes, and this refrigerator pipes is connected with the programmed cooling controller.
8. trifle limb class soil animal quantitative collection case according to claim 3 is characterized in that loading plate or thermal insulation layer outer and cabinet wall contact portion do not have the gap, and separated and collected device and bearing holes contact portion do not have the gap.
9. trifle limb class soil animal quantitative collection case according to claim 1 is characterized in that described bearing holes (8) is provided with the cover plate of flexible connection.
10. trifle limb class soil animal quantitative collection case according to claim 1 is characterized in that described collection ware (12) bottom is covered with gypsum/active carbon Mixed culture matrix or ethylene glycol.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310150590.1A CN103240137B (en) | 2013-04-27 | 2013-04-27 | Quantitative collection box of little arthropod soil animals |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310150590.1A CN103240137B (en) | 2013-04-27 | 2013-04-27 | Quantitative collection box of little arthropod soil animals |
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| Publication Number | Publication Date |
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| CN103240137A true CN103240137A (en) | 2013-08-14 |
| CN103240137B CN103240137B (en) | 2015-07-01 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201310150590.1A Expired - Fee Related CN103240137B (en) | 2013-04-27 | 2013-04-27 | Quantitative collection box of little arthropod soil animals |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104998707A (en) * | 2015-07-17 | 2015-10-28 | 河北爱能生物科技股份有限公司 | Unified liquid adding and changing oscillation shaking box applied in decellularization reaction process |
| CN106670088A (en) * | 2017-02-14 | 2017-05-17 | 中国科学院华南植物园 | Separation device for small animals in soil |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2762102Y (en) * | 2005-01-05 | 2006-03-01 | 中国科学院东北地理与农业生态研究所 | Soil animal separator |
| CN201048523Y (en) * | 2006-11-27 | 2008-04-23 | 新疆维吾尔自治区疾病预防控制中心 | Multifunctional small animal separator |
| KR20110039595A (en) * | 2009-10-12 | 2011-04-20 | 서울대학교산학협력단 | Pitpole Trap with Filter Module |
| CN102407172A (en) * | 2011-09-07 | 2012-04-11 | 中国科学院南京土壤研究所 | Portable soil animal collection device |
| CN202823413U (en) * | 2012-08-17 | 2013-03-27 | 乐山师范学院 | Portable soil animal collecting device |
| CN203227508U (en) * | 2013-04-27 | 2013-10-09 | 中国科学院南京土壤研究所 | Small-section limb-type soil animal separation and quantitative collection device |
-
2013
- 2013-04-27 CN CN201310150590.1A patent/CN103240137B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2762102Y (en) * | 2005-01-05 | 2006-03-01 | 中国科学院东北地理与农业生态研究所 | Soil animal separator |
| CN201048523Y (en) * | 2006-11-27 | 2008-04-23 | 新疆维吾尔自治区疾病预防控制中心 | Multifunctional small animal separator |
| KR20110039595A (en) * | 2009-10-12 | 2011-04-20 | 서울대학교산학협력단 | Pitpole Trap with Filter Module |
| CN102407172A (en) * | 2011-09-07 | 2012-04-11 | 中国科学院南京土壤研究所 | Portable soil animal collection device |
| CN202823413U (en) * | 2012-08-17 | 2013-03-27 | 乐山师范学院 | Portable soil animal collecting device |
| CN203227508U (en) * | 2013-04-27 | 2013-10-09 | 中国科学院南京土壤研究所 | Small-section limb-type soil animal separation and quantitative collection device |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104998707A (en) * | 2015-07-17 | 2015-10-28 | 河北爱能生物科技股份有限公司 | Unified liquid adding and changing oscillation shaking box applied in decellularization reaction process |
| CN106670088A (en) * | 2017-02-14 | 2017-05-17 | 中国科学院华南植物园 | Separation device for small animals in soil |
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| Publication number | Publication date |
|---|---|
| CN103240137B (en) | 2015-07-01 |
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