CN104998707B - Unified liquid adding and changing oscillation shaking box applied in decellularization reaction process - Google Patents
Unified liquid adding and changing oscillation shaking box applied in decellularization reaction process Download PDFInfo
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- CN104998707B CN104998707B CN201510421391.9A CN201510421391A CN104998707B CN 104998707 B CN104998707 B CN 104998707B CN 201510421391 A CN201510421391 A CN 201510421391A CN 104998707 B CN104998707 B CN 104998707B
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- 239000007788 liquid Substances 0.000 title claims abstract description 42
- 238000006243 chemical reaction Methods 0.000 title abstract description 16
- 230000010355 oscillation Effects 0.000 title abstract description 9
- 238000007599 discharging Methods 0.000 claims abstract description 9
- 238000007789 sealing Methods 0.000 claims abstract description 5
- 238000000034 method Methods 0.000 claims description 23
- 230000008569 process Effects 0.000 claims description 21
- 230000000694 effects Effects 0.000 claims description 19
- 241001125879 Gobio Species 0.000 claims description 9
- 239000011521 glass Substances 0.000 claims description 5
- 241001465754 Metazoa Species 0.000 abstract description 21
- 238000004519 manufacturing process Methods 0.000 abstract description 6
- 239000000376 reactant Substances 0.000 description 17
- 230000008859 change Effects 0.000 description 7
- 230000006872 improvement Effects 0.000 description 6
- 238000010828 elution Methods 0.000 description 4
- 230000001413 cellular effect Effects 0.000 description 3
- 239000000284 extract Substances 0.000 description 3
- 238000007689 inspection Methods 0.000 description 3
- 241000269799 Perca fluviatilis Species 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000004886 process control Methods 0.000 description 2
- 206010054949 Metaplasia Diseases 0.000 description 1
- 241001074085 Scophthalmus aquosus Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000015689 metaplastic ossification Effects 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
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Abstract
The invention relates to a unified liquid adding and changing oscillation shaking box device applied in a decellularization reaction process; the device includes a box body with the front end opening, the top of the box body is provided with a liquid adding pipe and a drain pipe, and the box body is provided with a conical bottom plate; a lowest position of the conical bottom plate is provided with a liquid discharging pipe, and a working zone is formed between the conical bottom plate and the top of the box body; the working zone inside the box body is provided with an object bearing bracket, the object bearing bracket is provided with a plurality of layers of slots, and plate and frame type adjustable fixtures are detachably inserted in the slots; the box body is hinged with a vertical plate used for making the working zone form a closed space and capable of quickly being opened and closed, the vertical plate is locked tightly with the box body through a locking device, and a sealing strip is arranged between the vertical plate and the box body. Compared with a traditional oscillation shaking table, the unified liquid adding and changing oscillation shaking box device has the following advantages that unified liquid adding and changing is achieved, a decellularization reaction system is uniform and constant, the size range of treated animal tissue samples is flexible and adjustable, the equal space processing ability is larger, and scaling and industrialized production is facilitated.
Description
Technical field
The present invention is to be related to device prepared by a kind of medical de- cellular system engineering support production, and specially one kind is applied to
The unification of de- cell effect process add change liquid vibration shake case apparatus.
Background technology
In the production preparation process of medical de- cellular system engineering support, core process is that the wash-out of zooblast is processed,
Main reaction mechanism is：Under the conditions of certain reaction temperature, animal tissue's print is immersed in reactant liquor, under oscillating condition
Reaction, and reactant liquor is replaced as frequently as, until print DNA residue detection results show and meet after quality standard, terminating reaction.Pass
The consersion unit of system is vibration shaking table, and processing procedure is：Animal tissue's print to be processed is respectively put into into one by one glass triangle
In bottle, then in triangular flask a certain amount of reactant liquor is added one by one, then triangular flask is fixed on one by one by spring perch and is shaken
On the support board of bed, then under certain temperature, oscillation amplitude and frequency condition, after the oscillating reactions regular hour, stop
Vibration, the reactant liquor being poured off one by one successively in each triangular flask, then new reactant liquor is changed to again, again by spring perch
Triangular flask is fixed on the support board of shaking table, oscillating reactions is then carried out again, so repeatedly, until the DNA of print
Residue detection is qualified, terminating reaction, into next program.This traditional vibration shaking table and de- cell effect process, it is only suitable
Close test and small-scale production and processing is used, it is impossible to meet the needs of large-scale production, and there is by hand plus change liquid consumption
When it is longer and be mingled with human factor, each triangular flask have a style of one's own reaction system heterogeneity, process print size be subject to triangular flask
Many unfavorable factors such as specification restriction, these exactly hamper medical de- cellular system engineering support scale, industrialized development
Bottleneck problem, and the present invention is exactly for solving the innovation done of these problems, it will to promoting medical de- groups of cells weaver
The scale of engineering support, industrialization production play positive effect.
The content of the invention
The technical problem to be solved is to provide one kind to be applied to the de- cell effect process of animal tissue's print
Unification add and change liquid vibration and shake case apparatus, it is possible to achieve it is unified plus change liquid operation, the reaction system of uniformity, scale
Production and processing.
In order to solve above-mentioned technical problem, the technical scheme that the present invention takes is as follows：
A kind of unification for being applied to de- cell effect process adds changes liquid vibration and shakes case apparatus, and it includes the case of front opening
Body, the riser being arranged at the casing front opening, the object carrying support being arranged in the casing and plate and frame flexible jig；
Liquid-feeding tube and evacuated tube are provided with the top of the casing, charging valve, the casing are provided with the liquid-feeding tube
Conical bottom board is provided with, the lowest point of the conical bottom board is provided with liquid discharging tube, bleeder valve is provided with the liquid discharging tube, described
Workspace is formed between conical bottom board and the casing top；
Multilayer slot is provided with the object carrying support, the plate and frame flexible jig detachable interposing is placed in the slot
It is interior；
It is hinged with the casing for making workspace form closing space and can be with rapidly opened and closed riser, the riser
Locked by locking device with the casing, sealing strip is provided between the riser and the casing, in the object carrying support
It is placed in the workspace.
Used as further improvement of the invention, the plate and frame flexible jig is made up of two n shape frames being mutually inserted.
As further improvement of the invention, on the n shapes frame compressing tablet is provided with.
Used as further improvement of the invention, the riser lower end is hinged by rotary shaft with the lower box, described
Riser upper end is connected by the locking device with the casing top.
Used as further improvement of the invention, riser lower end both sides are provided with gudgeon, and the institute casing both sides are arranged
There is the otic placode corresponding with the gudgeon, jack is provided with the otic placode, the gudgeon activity is inserted in the jack.
Used as further improvement of the invention, the locking device includes movable bolt and matched nut, institute
State casing both sides and be provided with movable bolt, one end of the movable bolt is hinged with the casing, the movable bolt it is another
Rotation on side is equipped with nut, and the both sides of the riser are provided with locking slot.
As further improvement of the invention, on the riser glass vision panel is provided with.
Compared with prior art, having the beneficial effect that acquired by the present invention：
Case is shaken in the unified liquid feeding vibration for being applied to the de- cell effect process of animal tissue's print provided by the present invention, realizes
The unification of animal tissue's print is added in de- cell effect changes liquid, meanwhile, also with de- cell effect system uniformity, place
The advantages of size range of reason animal tissue print is flexibly adjustable, equal spatial manipulation ability is bigger, more conducively scale, industry
Metaplasia is produced.
The plate and frame flexible jig of the present invention is made up of two n shape frames being mutually inserted, and can as needed be furthered or be drawn
The distance of remote two n shape frames, realizes the fixation to different size animal tissue print.Compressing tablet is provided with n shape frames, can be fine
Fixed animal tissue's print.
Riser lower end of the present invention is hinged by rotary shaft with the lower box, and riser upper end is by locking device and casing
Top connects, and is easy to open and fastens and shakes case.Locking device is arranged on the movable bolt of casing sidepiece using one end hinge, operation
Convenient locking is firm.
Glass vision panel, convenient observation box house situation, convenient operation are provided with the front end riser of the present invention.
Description of the drawings
Accompanying drawing 1 is structural representation of the invention；
Accompanying drawing 2 opens riser status architecture schematic diagram for the present invention；
Accompanying drawing 3 opens riser state front view for the present invention；
Accompanying drawing 4 is body structure schematic diagram of the present invention；
Accompanying drawing 5 is the structural representation of the object carrying support of the present invention；
Accompanying drawing 6 is the structural representation of the plate and frame flexible jig of the present invention；
In the accompanying drawings：
1 casing, 1-1 liquid-feeding tubes, 1-2 conical bottom boards, 1-3 liquid discharging tubes, 1-4 otic placodes, 1-5 jacks, 1-6 charging valves, 1-7 are put
Liquid valve, 1-8 evacuated tubes, 2 risers, 2-1 gudgeons, 2-2 locking slots, 2-3 windowpanes, 3 object carrying supports, 3-1 slots, 4 plate and frames can
Adjust fixture, 4-1n shape frames, 4-2 compressing tablets, 5 sealing strips, 6-1 movable bolts, 6-2 nuts, 100 workspaces.
Specific embodiment
Further details of narration is carried out to the present invention below in conjunction with Fig. 1 ~ Fig. 6.
As shown in figures 1-6, a kind of unification for being applied to the de- cell effect process of animal tissue's print adds and changes liquid vibration and shake
Case apparatus, it includes the casing 1 of front opening, and the top of the casing 1 is provided with liquid-feeding tube 1-1 and evacuated tube 1-8, described to add
Charging valve 1-6 is provided with liquid pipe 1-1, the casing 1 is provided with conical bottom board 1-2, and the lowest point of the conical bottom board 1-2 sets
Liquid discharging tube 1-3 is equipped with, bleeder valve 1-7, the conical bottom board 1-2 and the top of the casing 1 are provided with the liquid discharging tube 1-3
Between form workspace 100, the liquid discharging tube 1-3 extends through the side wall of the casing 1, and the bottom of casing 1 plays fixed support and makees
With.Top outside the two side of casing 1 is hinged with the movable bolt 6-1 that can be swung with horizontal direction, is nested with movable bolt 6-1
There is nut 6-2.Bottom outside the two side of casing 1 is provided with the otic placode 1-4 with jack 1-5.
The lower end both sides of riser 2 are provided with gudgeon 2-1, and the gudgeon 2-1 activities are inserted in the jack 1-5.It is described
The both sides of riser 2 are provided with locking slot 2-2, and the locking slot 2-2 is used for holding movable bolt 6-1, between riser 2 and casing 1
Sealing strip 5 is provided with, for the top of riser 2 and two side walls and casing 1 of conical bottom board 1-2 and casing 1 to be formed into envelope
The workspace 100 closed, is provided with glass vision panel 2-3, convenient observation on riser 2.
Object carrying support 3 is placed with workspace 100, as shown in figs. 4 and 5, multilayer is provided with the object carrying support 3 and is inserted
Groove 3-1, can plant plate and frame flexible jig 4 in the slot 3-1, the n shape frames that plate and frame flexible jig 4 is mutually inserted by two
4-1 is constituted, and the compressing tablet 4-2 for fixing animal tissue's print is provided with the n shapes frame.
The using method of the present invention is as follows：
According to the size of animal tissue's print to be dealt with, plate and frame flexible jig is adjusted, animal tissue's print is consolidated
Surely step up, plate and frame flexible jig is pushed in multilayer object carrying support, then object carrying support is put into shakes in case, fix, close
Outermost cant board, is snapped in locking slot with fixing bolt, tightens nut；Bleeder valve is closed, casing top charging valve is opened, is added
Reactant liquor, after reaching requirement liquid level, closes charging valve, after inspection is errorless, controls temperature, starts oscillation switch, starts reaction.
In course of reaction, when changing liquid, stop oscillation, open bleeder valve, put the reactant liquor shaken only in case, close bleeder valve,
New reactant liquor is added, oscillating reactions is then proceeded to；When changing liquid again, repeat aforesaid operations.
After reaching reaction end, net reactant liquor is put, open outermost cant board, take out object carrying support, extract plate and frame out one by one
Flexible jig, removes animal tissue's print, then carries out next processing routine.
Process to animal tissue's sample is specifically shown in following two examples：
Example 1
Animal tissue's print of working process 10cm*10cm, by adjusting plate and frame flexible jig, will be taken off at cell
Animal tissue's print of reason is flatly fixed with compressing tablet, then adjustment plate and frame flexible jig is pushed one by one into multilayer loading
In frame, then object carrying support is put into shakes case, fix, shut outermost cant board, good by bolt buckle fixing seal, closing is put
Liquid valve, opens the charging valve on case top, is put into reactant liquor, controls temperature, starts vibration.According to technological requirement, press to the stipulated time
Afterwards, stop oscillation, open bleeder valve, put net reactant liquor, close bleeder valve, add new reactant liquor, realization to change liquid again, continue
Vibration；When changing liquid again, repeat aforesaid operations, until elution of reactive terminates, put net reactant liquor, open outermost cant board, take out
Object carrying support support, extracts one by one plate and frame flexible jig out, removes animal tissue's print, and Jing inspections meet pilot process control will
Ask, be for further processing, an elution process is completed to this.
Example 2
Animal tissue's print of working process 10cm*20cm, by adjusting plate and frame flexible jig, will be taken off at cell
Animal tissue's print of reason is flatly fixed with compressing tablet, then adjustment plate and frame flexible jig is pushed one by one into multilayer loading
In frame, then object carrying support is put into shakes case, fix and shut outermost cant board, good by bolt buckle fixing seal, closing is put
Liquid valve, opens the charging valve on case top, is put into reactant liquor, controls temperature, starts vibration.According to technological requirement, press to the stipulated time
Afterwards, stop oscillation, open bleeder valve, put net reactant liquor, close bleeder valve, add new reactant liquor, realization to change liquid again, continue
Vibration；When changing liquid again, repeat aforesaid operations, until elution of reactive terminates, put net reactant liquor, open outermost cant board, take out
Object carrying support support, extracts one by one plate and frame flexible jig out, removes animal tissue's print, and Jing inspections meet pilot process control will
Ask, be for further processing, an elution process is completed to this.
The above embodiment is only the preferred embodiments of the present invention, and and the feasible enforcement of non-invention exhaustion.It is right
For persons skilled in the art, to any the showing done by it on the premise of without departing substantially from the principle of the invention and spirit
The change being clear to, all should be contemplated as falling with the claims of the present invention.
Claims (7)
1. a kind of unification for being applied to de- cell effect process adds and changes liquid vibration and shake case apparatus, it is characterised in that it includes that front end is opened
The casing (1) of mouth, the riser (2) being arranged at the casing (1) front opening, the loading being arranged in the casing (1)
Frame (3) and plate and frame flexible jig (4)；
Liquid-feeding tube (1-1) and evacuated tube (1-8) are provided with the top of the casing (1), are provided with the liquid-feeding tube (1-1) and are added
Liquid valve（1-6）, the casing (1) is provided with conical bottom board (1-2), and the lowest point of the conical bottom board (1-2) is provided with liquid discharging tube
(1-3), it is provided with bleeder valve on the liquid discharging tube (1-3)（1-7）, the conical bottom board (1-2) and the casing (1) top
Between formed workspace (100)；
Multilayer slot (3-1) is provided with the object carrying support (3), plate and frame flexible jig (4) detachable interposing is placed in institute
State in slot (3-1)；
The riser (2) for making workspace (100) form closing space, the riser (2) and institute are hinged with the casing (1)
State casing (1) to lock by locking device, between the riser (2) and the casing (1) sealing strip (5), the load are provided with
Thing support (3) is built in the workspace (100).
2. a kind of unification for being applied to de- cell effect process according to claim 1 adds and changes liquid vibration and shake case apparatus, its
It is characterised by, the plate and frame flexible jig (4) is made up of two n shape frames (4-1) being mutually inserted.
3. a kind of unification for being applied to de- cell effect process according to claim 2 adds and changes liquid vibration and shake case apparatus, its
It is characterised by, compressing tablet (4-2) is provided with the n shapes frame.
4. a kind of unification for being applied to de- cell effect process according to claim 1 adds and changes liquid vibration and shake case apparatus, its
It is characterised by, riser (2) lower end is hinged by rotary shaft with the casing (1) bottom, riser (2) upper end passes through institute
State locking device to be connected with the casing (1) top.
5. a kind of unification for being applied to de- cell effect process according to claim 1 adds and changes liquid vibration and shake case apparatus, its
It is characterised by, riser (2) the lower end both sides are provided with gudgeon (2-1), institute casing (1) both sides are provided with and the gudgeon
(2-1) corresponding otic placode (1-4), is provided with jack (1-5) on the otic placode (1-4), the gudgeon (2-1) activity is inserted in
In the jack (1-5).
6. a kind of unification for being applied to de- cell effect process according to claim 1 adds and changes liquid vibration and shake case apparatus, its
It is characterised by, the locking device includes movable bolt (6-1) and matched nut (6-2), the two of the casing (1)
Side is provided with movable bolt (6-1), and one end and the casing (1) of the movable bolt (6-1) are hinged, the movable bolt
(6-1) rotation on opposite side is equipped with nut (6-2), and the both sides of the riser (2) are provided with locking slot (2-2).
7. a kind of unification for being applied to de- cell effect process according to claim 1 adds and changes liquid vibration and shake case apparatus, its
It is characterised by, on the riser (2) glass vision panel (2-3) is provided with.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510421391.9A CN104998707B (en) | 2015-07-17 | 2015-07-17 | Unified liquid adding and changing oscillation shaking box applied in decellularization reaction process |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510421391.9A CN104998707B (en) | 2015-07-17 | 2015-07-17 | Unified liquid adding and changing oscillation shaking box applied in decellularization reaction process |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104998707A CN104998707A (en) | 2015-10-28 |
| CN104998707B true CN104998707B (en) | 2017-04-19 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510421391.9A Active CN104998707B (en) | 2015-07-17 | 2015-07-17 | Unified liquid adding and changing oscillation shaking box applied in decellularization reaction process |
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| Country | Link |
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| CN (1) | CN104998707B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107158464B (en) * | 2017-05-31 | 2020-10-30 | 陕西瑞盛生物科技有限公司 | Automatic fluid infusion formula system of taking off cells |
| CN113214977B (en) * | 2021-05-12 | 2022-04-29 | 忻州师范学院 | A biological laboratory uses an incubator shaker |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3430926A (en) * | 1967-09-12 | 1969-03-04 | New Brunswick Scientific Co | Counterweight system for shaker apparatus |
| DE3815528C1 (en) * | 1988-05-06 | 1989-08-10 | W.C. Heraeus Gmbh, 6450 Hanau, De | |
| CN103240137B (en) * | 2013-04-27 | 2015-07-01 | 中国科学院南京土壤研究所 | Quantitative collection box of little arthropod soil animals |
| CN203303878U (en) * | 2013-06-18 | 2013-11-27 | 北京贝丽莱斯生物化学有限公司 | Box-type vertical reciprocating temperature-control shaking table |
| CN204952937U (en) * | 2015-07-17 | 2016-01-13 | 河北爱能生物科技股份有限公司 | Be applied to unity of taking off cell reaction sequence and shake case with trading liquid vibration |
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2015
- 2015-07-17 CN CN201510421391.9A patent/CN104998707B/en active Active
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| Publication number | Publication date |
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| CN104998707A (en) | 2015-10-28 |
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| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A unified fluid adding and changing oscillating shaking box applied to the process of acellular reaction Effective date of registration: 20220706 Granted publication date: 20170419 Pledgee: China Everbright Bank Limited by Share Ltd. Shijiazhuang branch Pledgor: HEBEI AINENG BIOTECHNOLOGY Co.,Ltd. Registration number: Y2022130000046 |
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Date of cancellation: 20230809 Granted publication date: 20170419 Pledgee: China Everbright Bank Limited by Share Ltd. Shijiazhuang branch Pledgor: HEBEI AINENG BIOTECHNOLOGY Co.,Ltd. Registration number: Y2022130000046 |
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Denomination of invention: A Unified Adding and Changing Fluid Oscillating Shake Box Applied to the Process of Acellular Reaction Effective date of registration: 20230814 Granted publication date: 20170419 Pledgee: China Everbright Bank Limited by Share Ltd. Shijiazhuang branch Pledgor: HEBEI AINENG BIOTECHNOLOGY Co.,Ltd. Registration number: Y2023980052030 |
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Granted publication date: 20170419 Pledgee: China Everbright Bank Limited by Share Ltd. Shijiazhuang branch Pledgor: HEBEI AINENG BIOTECHNOLOGY Co.,Ltd. Registration number: Y2023980052030 |
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Denomination of invention: A unified shaking incubator for decellularization reaction process Granted publication date: 20170419 Pledgee: China Everbright Bank Limited by Share Ltd. Shijiazhuang branch Pledgor: HEBEI AINENG BIOTECHNOLOGY Co.,Ltd. Registration number: Y2024980034479 |
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