CN103232555A - Method for extracting pectin from lemon peel residues by utilizing enzymatic method - Google Patents
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Abstract
The invention discloses a method for extracting pectin from lemon peel residues by utilizing an enzymatic method. The method comprises the following steps of: pre-treating lemon peel residues; mixing the pre-treated lemon peel residues with a buffer solution having a pH value of 4-8 in a weight ratio of (1:15) to (1:30); adding 0.01%-0.3% of enzyme preparation in terms of the weight of the lemon peel residues for stirring and extracting for 1-5 hours under the temperature of 40 DEG C to 70 DEG C; and carrying out centrifugal separating, vacuum concentrating, cooling, discoloring, alcohol precipitating, sugar eliminating, drying and crushing in sequence to obtain a pectin powder finished product. According to the method for extracting pectin from lemon peel residues by utilizing the enzymatic method, the buffer solution with the pH value of 4-8 is added to be mixed with the enzyme preparation, so that the pectin extraction time is greatly shortened, the extraction efficiency is improved, the usage amount of the enzyme preparation is reduced, the extraction cost is lowered, and the pectin extraction rate and the product purity are further improved. Moreover, the used protease is mostly pure natural plant extracted from the plant, lower in price and more beneficial to realizing commercial application.
Description
Technical field
The invention belongs to and utilize Enzymatic Extraction pectin technical field, be specifically related to a kind of method of utilizing enzyme process from the lemon peel slag, to extract pectin.
Background technology
Along with modern nectar industrial expansion, a large amount of discarded pomace that produces in the course of processing of squeezing juice from fresh fruit fails to be utilized effectively for a long time, causes the huge waste of resource, and the while is serious environment pollution also.If these processing fruits wastes are carried out comprehensive process, resource is used adequately reasonably, improve the overall economic efficiency of processing fruits greatly, also can reduce the pollution to environment.Thereby a lot of scientific workers begin one's study and extract soluble dietary fibre from discarded pomaces---pectin.
Pectin is identified as one of 21st century food function composition, is called as " balancing agent of HUMAN HEALTH ".Pectin is mainly used in foodstuff additive, as thickening material, gelifying agent, stablizer, for fat agent, emulsifying agent etc., also can be used as water-soluble dietary fibre for reducing blood pressure and blood lipoid, promote the absorption of VITAMIN, prevent lead poisoning, prevent and treat health cares such as arteriosclerosis (Voragen AG J, et al. Struct Chem, 2009,20,263).
The report pectin starting material surpasses 40 kinds at present, but the main raw material of commodity pectin still is peel of Citrus reticulata Blanco and Pericarpium Mali pumilae (May CD. Carbohydrate Polymers, 1990,12 (1): 79).Lemon is a kind of good body-care fruit, has very high economic worth, the lemon processing of China mainly is products such as production lemon oil, lemon juice (lemon beverage), lemon dry plate, and based on roughing, so lemon produces 40 ~ 50% skin slag approximately after roughing.After deliberation, the lemon peel slag is rich in pectin, and than general Citrus raw material pectin content height, the orange peel residue pectin content only is 20%, and the pectin content of lemon peel slag can be up to 30%(Hai Xiang Wei etc. food research and development, 2006,27,157).Moreover, citrus pectin still be in all pectin performance best, color and luster the best, price be the highest pectin also, but regrettably at present domesticly also do not have a commercial citrus pectin.
The existing main method of extracting pectin have classical acid extraction method, ion exchange method, microwave method, microbial method, Continuous Countercurrent Extraction method, enzyme process etc. (Tian Hui etc. Food Additives Used in China, 2007, (1): 126).
Traditional method is adopted in the production of domestic goods pectin more---the acid extraction ethanol precipitation.Even so, but the shortcoming that sour formulation exists is tangible: 1) local hydrolysis easily takes place in pectin molecule in leaching process, can reduce the relative molecular weight of pectin, influences pectin yield and quality; Need use a large amount of mineral acids when 2) extracting, this can increase cost on the one hand, and acid solution is understood etching apparatus on the other hand, and environment is had pollution; 3) when extracting pectin with ethanol sedimentation, the amount of alcohol of consumption is too big, and the high and recycling difficulty of ethanol price makes the cost of extraction too high in addition, and economic benefit is not obvious.So people are devoted to acid technological process is improved.As (foodstuffs industry science and technology, 2007,28 (6): 182) just improved pretreatment process, namely adopted microwave deactivating enzyme and composite phosphate pre-treatment lemon peel, thereby improved the pectin yield such as Xu Dan.(food research and development, 2012,33 (9): 76) then adopt organic acid to extract pectin from lemon peel, reduced the erosion rate of acid solution to equipment effectively such as Liu Yiwu.It is raw material with the lemon peel that Wu Yongxian etc. disclose a kind of in CN 1107858, the method for acid extraction pectin.This method replaces vacuum concentration with ultrafiltration and concentration, has overcome the high shortcoming of vacuum concentration energy consumption.It is raw material with the lemon peel that Lv Weixue discloses a kind of in CN102731679, acid extraction, combining ultrafiltration nanofiltration, spraying drying are extracted the method for pectin, reduced the usage quantity of organic solvent, keep original biological activity, just can increase effective period of food quality, nourishing function etc. when pectin adds in the food like this.Masmoudi M etc. find again to extract pectin from lemon peel with acidifying jujube juice, can increase local flavor and the gellifying property of pectin from lemon peel and reduce pectin esterification degree (Carbohyd Polym, 2008,74,185; Food Sci Technol Int, 2010,16,105; Food Bioproc Technol 2012,5,687.).Studies show that by the composite phosphate pre-treatment of people such as Wan Guofu can effectively improve pectin from lemon peel extraction yield (foodstuffs industry science and technology, 27 (2): 78), and utilize ultrasonic wave, microwave-assisted can improve the pectin from lemon peel extraction yield (Wan Guofu etc. food research and development, 2006,27 (7): 115; Li Mingyuan. food research and development, 2007,28 (5): 91).
Ion exchange method production cost height needs higher temperature and long-time heating, and the pectin that contains in the raw material can produce sex change inevitably and decompose and destroy, and reduces the quality and quantity that pectin extracts.The microwave rule exists: microwave heating speed is too fast, wayward Heating temperature; The wavelength of microwave and power need strict control, otherwise can cause pectin product form difference to some extent; The microwave method misoperation is leaked easily, and biology is produced harm.The influence of microbial method extracts pectin when being subjected to pre-treatment, the reaction of raw material solid-to-liquid ratio, microbial growth time, soaking time and pH value is bigger.So these extracting method all are difficult to realize industrialization.
Enzymatic Extraction pectin has phase of history.In the phase at the end of the twenties in last century, Paul etc. just take off ester with Rohapect MPE and prepare low-ester pectin.Employing protopectinases such as Sakamoto extract pectin (Process Biochemistry, 1995,30 (5): 403).(Food Hydrocolloids such as Shkodina, 1998,12 (3): 313) then by relatively acid system and enzyme process (cellulase, hemicellulase and Glycosylase) extract pectin from pumpkin, the output of finding Enzymatic Extraction pectin all is higher than acid system, wherein the output of cellulase is the highest, be more than the twice of acid system, but the time reach 20 h.Zhang Yiqing etc. have studied with homemade protopectinase crude enzyme liquid (not having enzyme analysis content) and have extracted pectin in the Cirtus peel, and the pectin productive rate reaches the 35.7%(Food science, and 2005,26 (1): 150).What disclose recently utilizes pectin researchs such as cellulase extraction apple peel, orange peel, pineapple peel, orange peel and premna microphylla also more.A kind of method of utilizing cellulase, hemicellulase and mixed preparation thereof to extract pectin in citrus fruits, tropical fruit, apple, beet, Sunflower Receptacle, soybean and composition thereof is disclosed in CN 101160327 as Gu Zeke etc.But extraction time is longer, and the longest extraction time reaches 72h.Zhao Yue etc. disclose in CN 102161713 and have adopted cellulase, hemicellulase, Glycosylase and mixed enzyme thereof to prepare the method for high purity pectin continuously by enzymolysis, ultrafiltration and concentration, drying process with atomizing.Contreras-Esquivel etc. (Food Sci Biotechnol 2006,15,163) find that endo-polygalacturonase (genus polygalacturonase) is conducive to reduce the pectin from lemon peel gamma value.Though Enzymatic Extraction pectin is because of high efficiency and the specificity of enzyme effect, pectin yield height, the technological process of production and equipment are simple, technology is easy to control, and the degree of gelation that can keep product better, but because the enzyme that uses is mainly derived from microorganism culturing, so can be because of the difference of bacterial classification, the difference of culture condition makes the composition difference of enzyme, in addition the complex structure of enzyme itself, even if make that the extraction efficiency of using different manufacturers enzyme of the same race is also different, and both at home and abroad in the research of Enzymatic Extraction pectin, all ubiquity extracts the cost height with enzyme, be difficult to realize industrialization problem (Han Dongmei etc. foodstuffs industry science and technology, 2011,32 (2): 183).
Summary of the invention
The objective of the invention is from the lemon peel slag, to extract the problem that pectin exists at existing enzyme process, a kind of method of utilizing enzyme process to extract pectin from the lemon peel slag is provided.
A kind of method of utilizing enzyme process from the lemon peel slag, to extract pectin provided by the invention, processing step and the condition of this method are as follows:
1) the lemon peel slag is put into water in 2 ~ 3 times of tare weights, heated and boiled 5 ~ 10 min, oven dry, pulverize to colourless with the clear water rinsing;
2) be that 4 ~ 8 damping fluid mixes with the lemon peel slag pulverized by weight 1:15 ~ 1:30 and pH, add then in lemon peel slag weight 0.01 ~ 0.3% zymin and slowly stir, be incubated extraction 1 ~ 5 h down in 40 ~ 70 ℃;
3) with the pectin extracting soln centrifugation, gained pectin stoste is concentrated into and concentrates 1/4 ~ 1/2 of preceding extracting liquid volume, i.e. cooling fast;
4) hydrogen peroxide of adding fructose concentrating liquid quality meter 0.5 ~ 3% in fructose concentrating liquid, 30 ~ 60 min decolour under the room temperature, add 1 ~ 3 times of concentration then and be 95% ethanol, leave standstill 30 ~ 60 min, filter, 75% washing with alcohol that in filter cake, adds 2 ~ 3 times of weight again, wash repeatedly 3 ~ 5 times, filter, the washing with alcohol dehydration, drying is the pectin powder finished product after the pulverizing.
The used damping fluid of aforesaid method is that citric acid one citric acid is received or citric acid one Sodium phosphate dibasic is formed, and its ratio is 4 ~ 8 to be as the criterion with the pH regulator of damping fluid.
The used zymin of aforesaid method is at least a in cellulase, hemicellulase, papoid, bromeline and the ficin, the natural plant enzyme that preferably extracts from plant---at least a in papoid, bromeline and the ficin.
The lemon peel slag of pulverizing in the aforesaid method is that 6 ~ 8 damping fluid mixes by weight preferred 1:20 ~ 1:30 and pH.
The consumption of used enzyme preparation preferred 0.1 ~ 0.3% in the aforesaid method; Preferred 50 ~ 60 ℃ of holding temperature, preferred 2 ~ 3 h of soaking time; The hydrogen peroxide consumption is preferred 2.0%, preferred 50 ~ 60 min of bleaching time.
Used hydrogen peroxide is that commercially available concentration is 30% hydrogen peroxide in the aforesaid method.
Compared with prior art, advantage of the present invention is:
Since the inventive method when using zymin to extract pectin, also add pH and be 4 ~ 8 damping fluid and cooperated with it, thereby not only shortened the extraction time of pectin greatly, improved extraction efficiency, and also reduced the usage quantity of zymin, reduced extraction cost.
2. because the inventive method adopts the natural plant enzyme to extract pectin, under the prerequisite of the higher extraction yield of maintenance, not only the consumption of enzyme can reduce, and price is lower, thereby both can further reduce production costs, and can improve product purity height (〉=65%), make it to satisfy that China (GB 25533-2010) and European Union formulate that foodstuff additive---the standard of pectin directly comes into the market as products in circulation.
3. not only cost is low for the natural plant enzyme that adopts owing to the inventive method, and consumption is few, and its pectin extraction yield height, thereby the economic benefit of industrialization is very considerable.
4. adopt the medical disinfecting level ethanol of concentration 75% owing in the desugar technology of the inventive method, thereby reduced the influence to product such as microorganism, bacterium, further improved the pectin quality.
5. operation is simple for extracting method provided by the invention, is suitable for suitability for industrialized production.
Embodiment
Carry out concrete description below by the present invention of embodiment; be necessary to be pointed out that at this present embodiment only is used for the present invention is further specified; can not be interpreted as limiting the scope of the invention, the person skilled in the art in this field can make some nonessential improvement and adjustment according to the content of the invention described above.
What deserves to be explained is: the extraction yield of following each embodiment gained pectin, weight loss on drying, total galacturonic acid and gamma value are all measured according to the method for pectin analysis part among the GB 25533-2010.
Embodiment 1
The lemon peel slag is put into water in 2 times of tare weights, and heated and boiled 5 min to colourless, tentatively remove various non-colloidalmaterials such as aromatoising substance, sugar, bitter substance and pigment with the clear water rinsing to destroy the polygalacturonase in the lemon peel slag, oven dry, pulverize; Be that 5 citric acid one citric acid is received damping fluid and mixed with the lemon peel slag pulverized by weight 1:15 and pH, add then in lemon peel slag weight 0.01% cellulase and slowly stir, in 50 ℃ of insulation extraction 3 h down; The pectin extracting soln centrifugation is got pectin stoste; With the equal-volume water washing of pectin stoste, centrifugation again, merging filtrate, the filtrate after the merging is concentrated into and concentrates 1/2 of preceding extracting liquid volume, i.e. cooling fast; The hydrogen peroxide that in fructose concentrating liquid, adds fructose concentrating liquid quality meter 0.5%, decolouring 30 min under the room temperature add 3 times of concentration and are 95% ethanol then, leave standstill 30min, filter; In filter cake, add 75% washing with alcohol of 2 times of weight again, wash repeatedly 3 times, filter, 95% washing with alcohol dehydration, drying is the pectin powder finished product after the pulverizing.Collect filtrate, vacuum concentration reclaims ethanol and recycles.
The extraction yield of present embodiment pectin is 21.2%, weight loss on drying 7.0%, total galacturonic acid 70.7%, gamma value 71.1%.
Embodiment 2
The lemon peel slag is put into water in 2 times of tare weights, and heated and boiled 5 min, pulverize at oven dry; Be that 6 citric acid one citric acid is received damping fluid and mixed with the lemon peel slag pulverized by weight 1:30 and pH, add then in lemon peel slag weight 0.05% cellulase and slowly stir, in 40 ℃ of insulation extraction 4 h down; The pectin extracting soln centrifugation is got pectin stoste; With the equal-volume water washing of pectin stoste, centrifugation again, merging filtrate, the filtrate after the merging is concentrated into and concentrates 1/3 of preceding extracting liquid volume, i.e. cooling fast; The hydrogen peroxide that in fructose concentrating liquid, adds fructose concentrating liquid quality meter 1.0%, decolouring 40 min under the room temperature add 2 times of concentration and are 95% ethanol then, leave standstill 30min, filter; Add 75% washing with alcohol of 2 times of weight again in filter cake, wash repeatedly 4 times, filter, with 95% washing with alcohol dehydration, drying is the pectin powder finished product after the pulverizing.Collect filtrate, vacuum concentration reclaims ethanol and recycles.
The extraction yield of present embodiment pectin is 22.6%, weight loss on drying 7.2%, total galacturonic acid 70.3%, gamma value 71.0%.
Embodiment 3
The lemon peel slag is put into water in 2.3 times of tare weights, and heated and boiled 5 min, pulverize at oven dry; Be that 7 citric acid one Sodium phosphate dibasic damping fluid mixes with the lemon peel slag pulverized by weight 1:25 and pH, add then in lemon peel slag weight 0.1% ficin and slowly stir, in 70 ℃ of insulation extraction 5 h down; The pectin extracting soln centrifugation is got pectin stoste; With the equal-volume water washing of pectin stoste, centrifugation again, merging filtrate, the filtrate after the merging is concentrated into and concentrates 1/3 of preceding extracting liquid volume, i.e. cooling fast; The hydrogen peroxide that in fructose concentrating liquid, adds fructose concentrating liquid quality meter 2.0%, decolouring 50 min under the room temperature add 2 times of concentration and are 95% ethanol then, leave standstill 60min, filter; Add 75% washing with alcohol of 2.3 times of weight again in filter cake, wash repeatedly 5 times, filter, with 95% washing with alcohol dehydration, drying is the pectin powder finished product after the pulverizing.Collect filtrate, vacuum concentration reclaims ethanol and recycles.
The extraction yield of present embodiment pectin is 26.6%, weight loss on drying 7.6%, total galacturonic acid 70.4%, gamma value 60.2%.
Embodiment 4
The lemon peel slag is put into water in 2.6 times of tare weights, and heated and boiled 10 min, pulverize at oven dry; Be that 7.5 citric acid one Sodium phosphate dibasic damping fluid mixes with the lemon peel slag pulverized by weight 1:30 and pH, add then in lemon peel slag weight 0.3% ficin and slowly stir, in 55 ℃ of insulation extraction 2 h down; The pectin extracting soln centrifugation is got pectin stoste; With the equal-volume water washing of pectin stoste, centrifugation again, merging filtrate, the filtrate after the merging is concentrated into and concentrates 1/4 of preceding extracting liquid volume, i.e. cooling fast; The hydrogen peroxide that in fructose concentrating liquid, adds fructose concentrating liquid quality meter 2.0%, decolouring 50 min under the room temperature add 1 times of concentration and are 95% ethanol then, leave standstill 60min, filter; Add 75% washing with alcohol of 2.3 times of weight again in filter cake, wash repeatedly 4 times, filter, with 95% washing with alcohol dehydration, drying is the pectin powder finished product after the pulverizing.Collect filtrate, vacuum concentration reclaims ethanol and recycles.
The extraction yield of present embodiment pectin is 31.4%, weight loss on drying 7.8%, total galacturonic acid 66.0%, gamma value 63.4%.
Embodiment 5
The lemon peel slag is put into water in 3 times of tare weights, and heated and boiled 8 min, pulverize at oven dry; Be that 4 citric acid one Sodium phosphate dibasic damping fluid mixes with the lemon peel slag pulverized by weight 1:30 and pH, add then in lemon peel slag weight 0.2% hemicellulase and slowly stir, in 60 ℃ of insulation extraction 2 h down; The pectin extracting soln centrifugation is got pectin stoste; With the equal-volume water washing of pectin stoste, centrifugation again, merging filtrate, the filtrate after the merging is concentrated into and concentrates 1/2 of preceding extracting liquid volume, i.e. cooling fast; The hydrogen peroxide that in fructose concentrating liquid, adds fructose concentrating liquid quality meter 2.0%, decolouring 60 min under the room temperature add 1.5 times of concentration and are 95% ethanol then, leave standstill 40min, filter; Add 75% washing with alcohol of 2 times of weight again in filter cake, wash repeatedly 4 times, filter, with 95% washing with alcohol dehydration, drying is the pectin powder finished product after the pulverizing.Collect filtrate, vacuum concentration reclaims ethanol and recycles.
The extraction yield of present embodiment pectin is 23.6%, weight loss on drying 7.4%, total galacturonic acid 71.0%, gamma value 65.8%.
Embodiment 6
The lemon peel slag is put into water in 2 times of tare weights, and heated and boiled 7 min, pulverize at oven dry; Be that 8 citric acid one sodium citrate buffer solution mixes with the lemon peel slag pulverized by weight 1:15 and pH, add then in lemon peel slag weight 0.25% bromeline and slowly stir, in 45 ℃ of insulation extraction 3 h down; The pectin extracting soln centrifugation is got pectin stoste; With the equal-volume water washing of pectin stoste, centrifugation again, merging filtrate, the filtrate after the merging is concentrated into and concentrates 1/2 of preceding extracting liquid volume, i.e. cooling fast; The hydrogen peroxide that in fructose concentrating liquid, adds fructose concentrating liquid quality meter 2.0%, decolouring 60 min under the room temperature add 1.5 times of concentration and are 95% ethanol then, leave standstill 40min, filter; Add 75% washing with alcohol of 2.6 times of weight again in filter cake, wash repeatedly 3 times, filter, with 95% washing with alcohol dehydration, drying is the pectin powder finished product after the pulverizing.Collect filtrate, vacuum concentration reclaims ethanol and recycles.
The extraction yield of present embodiment pectin is 26.4%, weight loss on drying 7.3%, total galacturonic acid 67.8%, gamma value 68.4%.
Embodiment 7
The lemon peel slag is put into water in 2 times of tare weights, and heated and boiled 7 min, pulverize at oven dry; Be that 6.5 citric acid one sodium citrate buffer solution mixes with the lemon peel slag pulverized by weight 1:20 and pH, add then in lemon peel slag weight 0.1% papoid and slowly stir, in 65 ℃ of insulation extraction 1 h down; The pectin extracting soln centrifugation is got pectin stoste; With the equal-volume water washing of pectin stoste, centrifugation again, merging filtrate, the filtrate after the merging is concentrated into and concentrates 1/2 of preceding extracting liquid volume, i.e. cooling fast; The hydrogen peroxide that in fructose concentrating liquid, adds fructose concentrating liquid quality meter 2.0%, decolouring 55 min under the room temperature add 3 times of concentration and are 95% ethanol then, leave standstill 50min, filter; Add 75% washing with alcohol of 2.6 times of weight again in filter cake, wash repeatedly 3 times, filter, with 95% washing with alcohol dehydration, drying is the pectin powder finished product after the pulverizing.Collect filtrate, vacuum concentration reclaims ethanol and recycles.
The extraction yield of present embodiment pectin is 29.4%, weight loss on drying 7.1%, total galacturonic acid 67.6%, gamma value 65.5%.
Embodiment 8
The lemon peel slag is put into water in 2.6 times of tare weights, and heated and boiled 8 min, pulverize at oven dry; Be that 8 citric acid one Sodium phosphate dibasic damping fluid mixes with the lemon peel slag pulverized by weight 1:20 and pH, add then in lemon peel slag weight 0.05% cellulase and 0.05% papoid and slowly stir, in 55 ℃ of insulation extraction 2h down; The pectin extracting soln centrifugation is got pectin stoste; With the equal-volume water washing of pectin stoste, centrifugation again, merging filtrate, the filtrate after the merging is concentrated into and concentrates 1/3 of preceding extracting liquid volume, i.e. cooling fast; The hydrogen peroxide that in fructose concentrating liquid, adds fructose concentrating liquid quality meter 2.0%, decolouring 50 min under the room temperature add 2 times of concentration and are 95% ethanol then, leave standstill 50min, filter; Add 75% washing with alcohol of 3 times of weight again in filter cake, wash repeatedly 3 times, filter, with 95% washing with alcohol dehydration, drying is the pectin powder finished product after the pulverizing.Collect filtrate, vacuum concentration reclaims ethanol and recycles.
The extraction yield of present embodiment pectin is 33.4%, weight loss on drying 7.5%, total galacturonic acid 65.4%, gamma value 64.0%.
Embodiment 9
The lemon peel slag is put into water in 3 times of tare weights, and heated and boiled 10 min, pulverize at oven dry; Be that 6 citric acid one sodium citrate buffer solution mixes with the lemon peel slag pulverized by weight 1:20 and pH, add then in lemon peel slag weight 0.05% hemicellulase and 0.05% ficin and slowly stir, in 60 ℃ of insulation extraction 2.5h down; The pectin extracting soln centrifugation is got pectin stoste; With the equal-volume water washing of pectin stoste, centrifugation again, merging filtrate, the filtrate after the merging is concentrated into and concentrates 1/2 of preceding extracting liquid volume, i.e. cooling fast; The hydrogen peroxide that in fructose concentrating liquid, adds fructose concentrating liquid quality meter 2.0%, decolouring 50 min under the room temperature add 2 times of concentration and are 95% ethanol then, leave standstill 60min, filter; Add 75% washing with alcohol of 3 times of weight again in filter cake, wash repeatedly 3 times, filter, with 95% washing with alcohol dehydration, drying is the pectin powder finished product after the pulverizing.Collect filtrate, vacuum concentration reclaims ethanol and recycles.
The extraction yield of present embodiment pectin is 37.6%, weight loss on drying 7.6%, total galacturonic acid 65.1%, gamma value 62.4%.
Embodiment 10
The lemon peel slag is put into water in 3 times of tare weights, and heated and boiled 10 min, pulverize at oven dry; Be that 7 citric acid one Sodium phosphate dibasic damping fluid mixes with the lemon peel slag pulverized by weight 1:25 and pH, add then in lemon peel slag weight 0.1% papoid and 0.1% bromeline and slowly stir, in 50 ℃ of insulation extraction 3h down; The pectin extracting soln centrifugation is got pectin stoste; With the equal-volume water washing of pectin stoste, centrifugation again, merging filtrate, the filtrate after the merging is concentrated into and concentrates 1/3 of preceding extracting liquid volume, i.e. cooling fast; The hydrogen peroxide that in fructose concentrating liquid, adds fructose concentrating liquid quality meter 3.0%, decolouring 50 min under the room temperature add 2 times of concentration and are 95% ethanol then, leave standstill 60min, filter; Add 75% washing with alcohol of 3 times of weight again in filter cake, wash repeatedly 3 times, filter, with 95% washing with alcohol dehydration, drying is the pectin powder finished product after the pulverizing.Collect filtrate, vacuum concentration reclaims ethanol and recycles.
The extraction yield of present embodiment pectin is 36.9%, weight loss on drying 7.7%, total galacturonic acid 65.4%, gamma value 61.3%.
Claims (7)
1. method of utilizing enzyme process from the lemon peel slag, to extract pectin, processing step and the condition of this method are as follows:
1) the lemon peel slag is put into water in 2 ~ 3 times of tare weights, heated and boiled 5 ~ 10 min, oven dry, pulverize to colourless with the clear water rinsing;
2) be that 4 ~ 8 damping fluid mixes with the lemon peel slag pulverized by weight 1:15 ~ 1:30 and pH, add then in lemon peel slag weight 0.01 ~ 0.3% zymin and slowly stir, be incubated extraction 1 ~ 5 h down in 40 ~ 70 ℃;
3) with the pectin extracting soln centrifugation, gained pectin stoste is concentrated into and concentrates 1/4 ~ 1/2 of preceding extracting liquid volume, i.e. cooling fast;
4) in fructose concentrating liquid, add hydrogen peroxide in fructose concentrating liquid quality 0.5 ~ 3%, 30 ~ 60 min decolour under the room temperature, add 1 ~ 3 times of concentration then and be 95% ethanol, leave standstill 30 ~ 60 min, filter, 75% washing with alcohol that in filter cake, adds 2 ~ 3 times of weight again, wash repeatedly 3 ~ 5 times, filter, the washing with alcohol dehydration, drying is the pectin powder finished product after the pulverizing.
2. the method for utilizing enzyme process to extract pectin from the lemon peel slag according to claim 1, the used damping fluid of this method are that citric acid one citric acid is received or citric acid one Sodium phosphate dibasic is formed.
3. the method for utilizing enzyme process to extract pectin from the lemon peel slag according to claim 1 and 2, the used zymin of this method are at least a in cellulase, hemicellulase, papoid, bromeline and the ficin.
4. the method for utilizing enzyme process to extract pectin from the lemon peel slag according to claim 1 and 2, the used zymin of this method are at least a in papoid, bromeline and the ficin.
5. the method for utilizing enzyme process to extract pectin from the lemon peel slag according to claim 1 and 2, the lemon peel slag of pulverizing in this method are that 6 ~ 8 damping fluid mixes by weight 1:20 ~ 1:30 and pH.
6. the method for utilizing enzyme process to extract pectin from the lemon peel slag according to claim 3, the lemon peel slag of pulverizing in this method are that 6 ~ 8 damping fluid mixes by weight 1:20 ~ 1:30 and pH.
7. the method for utilizing enzyme process to extract pectin from the lemon peel slag according to claim 4, the lemon peel slag of pulverizing in this method are that 6 ~ 8 damping fluid mixes by weight 1:20 ~ 1:30 and pH.
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| CN103923226A (en) * | 2014-04-23 | 2014-07-16 | 赛珂睿德生物医药科技(上海)有限公司 | Method for pretreating raw material for preparing pectin |
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| CN104592415A (en) * | 2015-01-04 | 2015-05-06 | 砀山海升果胶有限责任公司 | Novel pretreating process of raw materials for producing pectin |
| CN107880151A (en) * | 2017-12-30 | 2018-04-06 | 烟台燕晟信息技术有限公司 | A kind of extracting method of pectin |
| CN108148149A (en) * | 2016-12-05 | 2018-06-12 | 漳州市皓康生物科技有限公司 | A kind of method of pectin in Enzymatic Extraction orange peel |
| CN108314748A (en) * | 2018-03-23 | 2018-07-24 | 仲恺农业工程学院 | A kind of aloe skin pectous extracting method |
| CN108424940A (en) * | 2018-04-13 | 2018-08-21 | 安徽宇宁果胶股份有限公司 | A kind of production method of the medicinal pectin of targeted therapy |
| CN108713763A (en) * | 2018-03-26 | 2018-10-30 | 内江师范学院 | A kind of high-quality lemon diet fibers complex enzyme preparation method |
| CN111543629A (en) * | 2020-04-07 | 2020-08-18 | 杭州莱恩纳尔生物科技有限公司 | Method for simultaneously preparing pectin, fruit vinegar, ferment, probiotics and carbon black from peel residues |
| CN113712189A (en) * | 2021-08-27 | 2021-11-30 | 南宁学院 | Bio-enzyme extraction process for pectin in peel of pawpaw |
| CN115417936A (en) * | 2022-10-23 | 2022-12-02 | 华南理工大学 | Method for extracting pectin from waste beet pulp after sugar production by double-enzyme method |
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| US20120190831A1 (en) * | 2011-01-25 | 2012-07-26 | Cp Kelco Aps | Methods for Steam Flash Extraction of Pectin |
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Cited By (16)
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| CN103467619A (en) * | 2013-09-23 | 2013-12-25 | 安徽宇宁生物科技有限公司 | Method for decoloring pectin |
| CN103641930A (en) * | 2013-11-27 | 2014-03-19 | 威海市桢昊生物技术有限公司 | Method for extraction of pectin from pineapple peels and residue by enzymatic process |
| CN103641930B (en) * | 2013-11-27 | 2016-09-07 | 威海市桢昊生物技术有限公司 | A kind of method utilizing enzyme process to extract pectin from pineapple peel, slag |
| CN103923226A (en) * | 2014-04-23 | 2014-07-16 | 赛珂睿德生物医药科技(上海)有限公司 | Method for pretreating raw material for preparing pectin |
| CN103923226B (en) * | 2014-04-23 | 2016-08-24 | 赛珂睿德生物医药科技(上海)有限公司 | A kind of preprocessing method of raw materials prepared for pectin |
| CN104353104A (en) * | 2014-11-10 | 2015-02-18 | 苏州蔻美新材料有限公司 | Breathable medical gel material free of side effects and preparation method thereof |
| CN104592415A (en) * | 2015-01-04 | 2015-05-06 | 砀山海升果胶有限责任公司 | Novel pretreating process of raw materials for producing pectin |
| CN108148149A (en) * | 2016-12-05 | 2018-06-12 | 漳州市皓康生物科技有限公司 | A kind of method of pectin in Enzymatic Extraction orange peel |
| CN107880151A (en) * | 2017-12-30 | 2018-04-06 | 烟台燕晟信息技术有限公司 | A kind of extracting method of pectin |
| CN108314748A (en) * | 2018-03-23 | 2018-07-24 | 仲恺农业工程学院 | A kind of aloe skin pectous extracting method |
| CN108713763A (en) * | 2018-03-26 | 2018-10-30 | 内江师范学院 | A kind of high-quality lemon diet fibers complex enzyme preparation method |
| CN108424940A (en) * | 2018-04-13 | 2018-08-21 | 安徽宇宁果胶股份有限公司 | A kind of production method of the medicinal pectin of targeted therapy |
| CN111543629A (en) * | 2020-04-07 | 2020-08-18 | 杭州莱恩纳尔生物科技有限公司 | Method for simultaneously preparing pectin, fruit vinegar, ferment, probiotics and carbon black from peel residues |
| CN113712189A (en) * | 2021-08-27 | 2021-11-30 | 南宁学院 | Bio-enzyme extraction process for pectin in peel of pawpaw |
| CN115417936A (en) * | 2022-10-23 | 2022-12-02 | 华南理工大学 | Method for extracting pectin from waste beet pulp after sugar production by double-enzyme method |
| CN115417936B (en) * | 2022-10-23 | 2023-04-07 | 华南理工大学 | Method for extracting pectin from waste beet pulp after sugar production by double-enzyme method |
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