CN115417936B - Method for extracting pectin from waste beet pulp after sugar production by double-enzyme method - Google Patents
Method for extracting pectin from waste beet pulp after sugar production by double-enzyme method Download PDFInfo
- Publication number
- CN115417936B CN115417936B CN202211298443.4A CN202211298443A CN115417936B CN 115417936 B CN115417936 B CN 115417936B CN 202211298443 A CN202211298443 A CN 202211298443A CN 115417936 B CN115417936 B CN 115417936B
- Authority
- CN
- China
- Prior art keywords
- pectin
- beet pulp
- drying
- sugar
- buffer solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0045—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid alpha-D-Galacturonans, e.g. methyl ester of (alpha-1,4)-linked D-galacturonic acid units, i.e. pectin, or hydrolysis product of methyl ester of alpha-1,4-linked D-galacturonic acid units, i.e. pectinic acid; Derivatives thereof
- C08B37/0048—Processes of extraction from organic materials
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention belongs to the technical field of plant component extraction, and discloses a method for extracting pectin from waste beet pulp after sugar production by a two-enzyme method. The method comprises the following steps: cleaning beet pulp, drying, pulverizing, extracting at high temperature by steaming, adding the mixture into cellulose complex enzyme solution, performing enzymatic extraction to obtain beet pectin solution, adding 95% ethanol for precipitation, washing the precipitate with ethanol-water solution, and oven drying to obtain beet pectin. Compared with the acid extraction method, the oxalic acid-ammonium oxalate extraction method, the fermentation method and the like in the prior art, the pectin extraction rate is improved from 10-12% to 13-19%, and the emulsifying activity and the emulsifying stability of the beet pectin are improved.
Description
Technical Field
The invention relates to the technical field of plant component extraction, in particular to a method for extracting pectin from waste beet pulp after sugar refining by a double-enzyme method.
Background
Pectin has excellent gelling, thickening, emulsifying, stabilizing and other functional properties, and is recommended by the food additive association committee as a safe natural food additive without limiting the daily allowable intake. According to the degree of esterification, pectins can be classified into high-ester pectins and low-ester pectins. Most natural pectins are high-ester pectins and few low-ester pectins. Because the pectin can be gelled under the condition of low sugar or sugar-free, the low-ester pectin is widely used for producing low-sugar jam, jelly, yoghourt or sugar-free health food and the like. However, due to the limitation of production technology, the yield of low-ester pectin in China is far less than the demand amount, and the low-ester pectin still depends on foreign import so far, thereby seriously restricting the development of related food industries in China.
Pectin is one of the main active ingredients in beet pulp. The beet pulp is used as the main byproduct of beet sugar production, and the pectin content reaches 25 percent. The process of converting low cost into high value-added products is realized by extracting pectin from beet pulp. Because of its very good emulsifiability, beet pectin can be used in the preparation of milk beverages, and because of its many properties, it is widely used as a food additive in the baking industry and jam production. Pectin is widely present in the cell wall of plants, and is one of the supporting substances and plant cell wall components inside plant cells. The principle of extracting pectin from plant cell walls is to break the cell wall structure of the plant in the presence of an extractant or a certain physical action to separate pectin bound to other polysaccharides in the cell wall.
The pectin extracting method mainly comprises the traditional acid heat extraction method, ultrasonic wave auxiliary extraction method, enzyme extraction method, chelating agent extraction method and the like. However, the traditional acid-heat extraction method has larger damage degree to pectin and is easy to cause pollution, and compared with the traditional acid extraction method, the enzyme method has mild conditions and no pollution.
In addition, we have found that drying takes a lot of time during the experiment, and the material needs to be transferred after drying is completed, so that time is wasted and the possibility of material pollution is possibly existed.
Disclosure of Invention
The purpose of the invention is as follows: in order to provide a better two-enzyme method for extracting pectin from beet pulp discarded after sugar refining, a specific purpose is to see a plurality of substantial technical effects of a specific implementation part.
In order to achieve the purpose, the invention adopts the following technical scheme:
the method for extracting pectin from the waste beet pulp after sugar production by the double-enzyme method is characterized by comprising the following steps:
(1) Cleaning sugar beet pulp, removing impurities, drying, and pulverizing;
(2) Adding the crushed beet pulp powder into a buffer solution with the pH value of 4.0-5.0, wherein the buffer solution is a citric acid-sodium citrate buffer solution, then integrally placing the buffer solution into an autoclave for high-temperature cooking, and taking out the buffer solution after the pressure and temperature reduction are finished to room temperature; the high-temperature cooking conditions are that the solid-to-liquid ratio of the beet pulp is 1; the pressure is 50kpa;
(3) Taking out the mixture obtained in the step (2), adjusting the pH value to be 4.0-5.0, adding a mixed solution of cellulase and hemicellulase, stirring and extracting, heating the solution to inactivate the enzyme, filtering and separating to realize primary impurity removal, wherein undersize products obtained by filtering and separating are solid residues, and a beet pulp pectin solution and the solid residues obtained by enzymatic extraction are obtained; the enzyme adding amount is 40-100 mu L, and the ratio of the cellulase to the hemicellulase is 2:1, the extraction temperature is 45-55 ℃, the extraction mode is that the extraction is carried out in a water bath kettle under stirring, and the extraction time is 1-2h;
(4) Centrifuging the pectin solution obtained in the step (3) to remove protein impurities, removing impurities for the second time, taking the centrifuged supernatant, adjusting the pH to 2.0-3.0, adding 95% ethanol while stirring, standing until pectin is separated out, filtering with filter cloth, and washing with ethanol-water solution to obtain beet pectin; the centrifugation conditions in the step (4) are 4 ℃,8000rpm and 20min; adjusting pH with citric acid solution, and adding 95% ethanol with 3 times of supernatant volume; the ethanol-water solution washing refers to washing with 75% and 95% ethanol respectively, and the ethanol washing is to remove alcohol-soluble impurities, which is the third impurity removal.
The further technical scheme of the invention is that the drying is air blast drying until the moisture content is below 8%.
The invention further adopts the technical scheme that the filter cloth used in the filtering in the step (3) is 400-mesh filter cloth.
The further technical scheme of the invention is that the drying, the crushing and the adding of the citric acid-sodium citrate buffer solution are completed by special machines in batch production;
batch production's purpose-built machinery is dedicated dry crushing compounding all-in-one, dry crushing compounding all-in-one structure as follows:
a circumferential ventilation structure and an internal multipurpose drying and cutting structure which are arranged concentrically are arranged;
the circumferential ventilation structure is a cylindrical structure with a circular section, a cavity 11 in the cylindrical structure is connected with a fan ventilation opening 6, and a circle of air dispersing openings 7 are arranged on the inner wall of the cylindrical structure;
the multipurpose drying and cutting structure comprises a perforated cylinder 1, an opening is arranged above the perforated cylinder 1, the body of the perforated cylinder 1 is cylindrical, an arc-shaped bottom wall is arranged below the body of the perforated cylinder 1, and a plurality of powder through holes are formed in the body and the bottom wall of the perforated cylinder 1; still contain motor shaft 3 that can rotate at the middle part of taking a hole section of thick bamboo 1, arranged multiunit cutting stirring piece 4 on motor shaft 3, motor shaft 3 is connecting motor 2.
The further technical proposal of the invention is that the plurality of groups of cutting and stirring blades 4 are blades with blades.
The technical scheme is that a material powder storage area 8 is arranged below the circumferential ventilation structure, the side of the material powder storage area 8 is connected with a buffer solution injection port 9, and a mixture outlet pipe 10 is arranged below the material powder storage area 8.
The further technical scheme of the invention is that a weight sensor is arranged in the material powder storage area 8, and a flow meter is arranged on a pipeline of the buffer solution injection port 9.
A further technical solution of the present invention consists in that the mixture outlet pipe 10 is connected to an autoclave.
The invention further adopts the technical scheme that beet pulp is put into a perforated cylinder, then air is blown through an air vent of a fan, the air is diffused from a cavity through an air diffuser and blown to the material in the perforated cylinder, then the material is dried, in the drying process, a motor drives a cutting stirring sheet to be properly stirred, after the material is dried, the power of the motor is increased, the cutting stirring sheet is enabled to rotate at a high speed, the material is crushed to a proper particle size and falls down through a powder through hole of the perforated cylinder, the material enters a material powder storage area, then a buffer solution is put into the material powder storage area through a buffer solution injection port, and a mixture outlet pipe enters the next process.
Compared with the prior art, the invention adopting the technical scheme has the following beneficial effects: (1) The invention has high pectin extraction rate, low esterification degree, small relative molecular weight and light color; the content of galactaric acid of the pectin is high;
(2) The invention firstly provides a two-step method for extracting beet pulp pectin, namely high-temperature cooking and an enzyme method, compared with the acid-heat method, a chelating agent method and the like in the prior art, the extraction rate of the pectin is improved from 10-12 percent to 13-17 percent, and the emulsifying activity and the emulsifying stability are improved;
(3) Compared with the prior art, the extraction method has the advantages of no pollution and low pectin damage degree.
(4) The large-scale production and the drying and crushing are integrated; no complicated material is used for transferring. Can realize continuous industrial production, basically does not need a belt conveyor to transport materials after drying, and can realize the configuration of automatic buffer solution and powder.
Drawings
To further illustrate the present invention, further description is provided below with reference to the accompanying drawings:
FIG. 1 is a graph showing the GPC molecular weight measurement results of beet pulp pectin obtained in example 1 of the present invention;
FIG. 2 is a spectrum of an infrared scanning spectrum of beet pulp pectin obtained in example 1 of the present invention;
FIG. 3 is a galactonic acid map of beet pulp pectin obtained in example 1 according to the present invention;
FIG. 4 is a graph showing the distribution of particle sizes of the beet pulp emulsions prepared by the application example of the present invention after 0, 1, 3, 7 and 14 days at normal temperature; along with the prolonging of the storage time, the emulsion has slight destabilization, the single peak of the particle size distribution slightly deviates in the direction, but the distribution has little change on the whole, the phenomenon of serious rupture and destabilization does not occur, and the stability is better;
FIG. 5 is an optical microscope photograph of an emulsion prepared from beet pulp obtained in the example of the application of the present invention;
FIG. 6 is an appearance diagram of an emulsion prepared from beet pulp obtained in the application example of the present invention;
FIG. 7 is a schematic illustration of a combination machine of step one and step two;
FIG. 8 is a cross-sectional schematic view of FIG. 7;
FIG. 9 is a schematic view from another perspective of FIG. 7;
wherein: 1. a perforated cylinder; 2. a motor; 3. a motor shaft; 4. cutting the stirring sheet; 5. a cavity; 6. a fan vent; 7. an air diffuser; 8. a material powder storage area; 9. a buffer solution injection port; 10. a mixture outlet pipe; 11. a cavity.
Detailed description of the preferred embodiments
The present invention will be further illustrated with reference to the accompanying drawings and detailed description, which will be understood as being illustrative only and not limiting in scope. In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", "top", "bottom", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention. Furthermore, unless expressly stated or limited otherwise, the terms "mounted," "connected," and "coupled" are to be construed broadly and encompass, for example, both fixed and removable coupling as well as integral coupling; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
The patent provides a plurality of parallel schemes, and different expressions belong to an improved scheme based on a basic scheme or a parallel scheme. Each solution has its own unique features. Furthermore, the technical features involved in the different embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other. The fixing mode which is not described in the text can be any one of the fixing modes such as thread fixing, bolt fixing or glue bonding.
Examples
Cleaning sugar-processed beet pulp with clear water for several times, removing impurities, air drying at 50 deg.C for 12 hr, mechanically pulverizing dried beet pulp with a pulverizer for 30min to obtain beet pulp fine powder, sieving with 60 mesh sieve, sealing in sample bag, and storing at room temperature; adding the crushed and sieved beet pulp powder into the mixture according to the solid-liquid ratio of 1:20 Adding 600mL of citric acid-sodium citrate buffer solution (pH 4.5) according to the proportion of (W/V, g/mL), uniformly stirring, wrapping with gauze, wrapping with newspaper, sealing with cotton rope, placing into an autoclave, setting at 121 ℃ for 15min, reducing pressure, cooling to 100 ℃, taking out, and cooling in ice water bath. After cooling, adjusting the pH value to 4.5 by using citric acid, adding 0.6g of cellulase and 0.3g of hemicellulase, uniformly stirring, carrying out water bath at 50 ℃ and 270rpm for 2h, heating the extract to 100 ℃, continuously boiling for 5min for inactivating enzyme, quickly taking out the extract, cooling to room temperature, filtering by using 400-mesh filter cloth, centrifuging the pectin crude extract at 8000rpm/min, 20min and 4 ℃, adjusting the pH value of the supernatant to 3.0 by using citric acid, adding three times of 95% ethanol solution in volume while stirring, standing at room temperature for 1h, filtering by using 400-mesh filter cloth, washing by using 75% and 95% ethanol aqueous solution for 2 times in sequence, and drying in a drying box at 40 ℃ to constant weight to obtain the beet pulp pectin. Tests show that the beet pulp pectin yield is 14% (W/W, g/g), the uronic acid content is 68%, and the molecular weight is 312586Da.
Examples
Cleaning sugar-processed beet pulp with clear water for several times, removing impurities, air drying at 50 deg.C for 12 hr, mechanically pulverizing dried beet pulp with a pulverizer for 30min to obtain beet pulp fine powder, sieving with 60 mesh sieve, sealing in sample bag, and storing at room temperature; adding the crushed and sieved beet pulp powder into the mixture according to the solid-liquid ratio of 1:20 Adding 600mL of citric acid-sodium citrate buffer solution (pH 4.5) according to the proportion of (W/V, g/mL), uniformly stirring, wrapping with gauze, wrapping with newspaper, sealing with cotton rope, placing into an autoclave, setting at 121 ℃ for 15min, reducing pressure, cooling to 100 ℃, taking out, and cooling in ice water bath. After cooling, adjusting the pH value to 4.5 by using citric acid, adding 0.6g of cellulase and 0.3g of hemicellulase, uniformly stirring, carrying out water bath at 50 ℃ and 270rpm for 1h, heating the extract to 100 ℃, continuously boiling for 5min for inactivating enzyme, quickly taking out the extract, cooling to room temperature, filtering by using 400-mesh filter cloth, centrifuging the pectin crude extract at 8000rpm/min, 20min and 4 ℃, adjusting the pH value of the supernatant to 3.0 by using citric acid, adding three times of 95% ethanol solution in volume while stirring, standing at room temperature for 1h, filtering by using 400-mesh filter cloth, washing by using 75% and 95% ethanol aqueous solution for 2 times in sequence, and drying in a drying box at 40 ℃ to constant weight to obtain the beet pulp pectin. Tests show that the beet pulp pectin yield is 13.22% (W/W, g/g), the uronic acid content is 67%, and the molecular weight is 309256Da.
Examples
Cleaning sugar beet pulp with clear water for several times, removing impurities, air drying at 50 deg.C for 12 hr, mechanically pulverizing dried beet pulp with pulverizer for 30min to obtain beet pulp fine powder, sieving with 60 mesh sieve, sealing in sample bag, and storing at room temperature; adding the crushed and sieved beet pulp powder into the mixture according to the solid-liquid ratio of 1:20 Adding 600mL of citric acid-sodium citrate buffer solution (pH 4.5) according to the proportion of (W/V, g/mL), uniformly stirring, wrapping with gauze, wrapping with newspaper, sealing with cotton rope, placing into an autoclave, setting at 121 ℃ for 15min, reducing pressure, cooling to 100 ℃, taking out, and cooling in ice water bath. After cooling, adjusting the pH value to 4.5 by using citric acid, adding 0.6g of cellulase and 0.6g of hemicellulase, uniformly stirring, carrying out water bath at 50 ℃ and 270rpm for 1h, heating the extract to 100 ℃, continuously boiling for 5min for inactivating enzyme, quickly taking out an ice water bath, cooling to room temperature, filtering by using 400-mesh filter cloth, centrifuging the pectin crude extract at 8000rpm/min, 20min and 4 ℃, adjusting the pH value of a supernatant to 3.0 by using citric acid, adding 95% ethanol solution with three times of volume while stirring, standing at room temperature for 1h, filtering by using 400-mesh filter cloth, washing by using 75% and 95% ethanol aqueous solution for 2 times in sequence, and drying to constant weight in a drying box at 40 ℃ to obtain the beet pulp pectin. Tests show that the pectin yield of the beet pulp is 13 percent (W/W, g/g), the uronic acid content is 68 percent, and the molecular weight is 326025Da.
0.5g of pectin obtained in inventive example 1 was dissolved in 25ml of citric acid buffer (pH 3.5, concentration 50Mm, sodium benzoate content 0.1%), stirred overnight to dissolve it sufficiently, 7.5g of medium-chain fatty acid was added, and citric acid buffer was added to 50g. Pre-homogenizing with a handheld high-speed shearing machine at 20000rpm for 2 min, and further homogenizing with an ultrasonic cell disruptor at 3min,2s/2s. Storing the emulsion at normal temperature, measuring the particle diameter of the emulsion on 0, 1, 3, 5, 7 and 14 days, and taking an optical microscope picture and an appearance picture.
The emulsion in the application example has slight deviation of a single peak of the emulsion particle size distribution to a large particle size direction, a small amount of large-particle-size droplets appear after the storage time exceeds 3 days, the proportion of the droplets slowly increases along with the time, and although the changes occur in the storage process, the emulsion particle size distribution has small change on the whole, no serious fracture instability phenomenon occurs, and the stability is better.
It should be noted that the plurality of schemes provided in this patent include their own basic schemes, which are independent of each other and are not restricted to each other, but they may be combined with each other without conflict, so as to achieve a plurality of effects.
Example four: as a further improvement or a parallel scheme or an alternative independent scheme, the drying, the crushing and the adding of the subsequent citric acid-sodium citrate buffer solution are completed by a special machine for batch production;
the special machinery of batch production is dedicated dry crushing compounding all-in-one, dry crushing compounding all-in-one structure as follows:
a circumferential ventilation structure and an internal multipurpose drying and cutting structure which are arranged concentrically are arranged;
the circumferential ventilation structure is a cylindrical structure with a circular section, a cavity 11 in the cylindrical structure is connected with a fan ventilation opening 6, and a circle of air dispersing openings 7 are arranged on the inner wall of the cylindrical structure;
the multipurpose drying and cutting structure comprises a perforated cylinder 1, an opening is arranged above the perforated cylinder 1, the body of the perforated cylinder 1 is cylindrical, an arc-shaped bottom wall is arranged below the body of the perforated cylinder 1, and a plurality of powder through holes are formed in the body and the bottom wall of the perforated cylinder 1; still contain motor shaft 3 that can rotate at the middle part of taking a hole section of thick bamboo 1, arranged multiunit cutting stirring piece 4 on motor shaft 3, motor shaft 3 is connecting motor 2. The technical scheme of the invention has the following substantial technical effects and the realization process: beet pulp is put into a perforated cylinder, then the fan vent blows, wind distributes through the scattered wind gap in the cavity and blows to the material in the perforated cylinder after the perforated cylinder, carry out the drying, dry in-process, the motor drives the cutting stirring piece and suitably stirs, after the drying, increase motor power afterwards, let the cutting stirring piece rotate at a high speed, the material is smashed and is fallen down in the powder clearing hole through the perforated cylinder after suitable particle diameter, get into the material powder and deposit the region, put into buffer solution through the buffer solution filling opening afterwards, the mixture outlet pipe gets into next process.
The large-scale production and the drying and crushing are integrated; no complicated material is used for transferring. Can realize continuous industrial production, basically does not need a belt conveyor to transport materials after drying, and can realize the configuration of automatic buffer solution and powder. Is an important step of the laboratory to the industrial production.
Example five: as a further modification or a side-by-side or an alternative independent solution, the plurality of cutting and stirring blades 4 are blades with blades. The technical scheme of the invention has the following substantial technical effects and the realization process thereof: can conveniently cut.
Example six: as a further improvement or a parallel scheme or an alternative independent scheme, a material powder storage area 8 is arranged below the circumferential ventilation structure, the side of the material powder storage area 8 is connected with a buffer solution injection port 9, and a mixture outlet pipe 10 is further arranged below the material powder storage area 8.
Example seven: as a further modification or a side-by-side or alternatively an independent solution, a weight sensor is disposed in the material powder storage area 8, and a flow meter is disposed on a pipe of the buffer solution injection port 9. The mixture outlet pipe 10 is connected to an autoclave.
The foregoing shows and describes the general principles, principal features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are intended to illustrate the principles of the invention, but that various changes and modifications may be made without departing from the spirit and scope of the invention, and the invention is to be limited to the embodiments described above.
Claims (8)
1. The method for extracting pectin from the waste beet pulp after sugar production by the double-enzyme method is characterized by comprising the following steps:
(1) Cleaning beet pulp after sugar production, removing impurities, drying, and pulverizing;
(2) Adding the crushed beet pulp powder into a buffer solution with the pH value of 4.0-5.0, wherein the buffer solution is a citric acid-sodium citrate buffer solution, then integrally placing the whole into an autoclave for high-temperature cooking, and taking out after the pressure reduction and temperature reduction are finished to room temperature; the high-temperature cooking conditions are that the solid-to-liquid ratio of the beet pulp is 1; the pressure is 50kpa;
(3) Taking out the mixture obtained in the step (2), adjusting the pH value to be 4.0-5.0, adding a mixed solution of cellulase and hemicellulase, stirring and extracting, heating the solution to inactivate enzyme and filtering and separating to realize first impurity removal, wherein undersize materials obtained by filtering and separating are solid residues, and a beet pulp pectin solution and the solid residues obtained by enzymatic extraction are obtained; the enzyme adding amount is 40-100 mu L, and the ratio of the cellulase to the hemicellulase is 2:1, the extraction temperature is 45-55 ℃, the extraction mode is that the extraction is carried out in a water bath kettle under stirring, and the extraction time is 1-2h;
(4) Centrifuging the pectin solution obtained in the step (3) to remove protein impurities, realizing secondary impurity removal, taking a centrifuged supernatant, adjusting the pH to 2.0-3.0, adding 95% ethanol while stirring, standing until pectin is separated out, filtering with a filter cloth, and washing with an ethanol-water solution to obtain beet pectin; the centrifugation conditions in the step (4) are 4 ℃,8000rpm and 20min; adjusting pH with citric acid solution, adding 95% ethanol 3 times of supernatant volume; the ethanol-water solution washing refers to washing with 75% and 95% ethanol respectively, and the ethanol washing is to remove alcohol-soluble impurities, namely, removing impurities for the third time;
the drying, the crushing and the adding of the subsequent citric acid-sodium citrate buffer solution are completed by a special machine for batch production;
batch production's purpose-built machinery is dedicated dry crushing compounding all-in-one, dry crushing compounding all-in-one structure as follows:
a circumferential ventilation structure and an internal multipurpose drying and cutting structure which are arranged concentrically are arranged;
the circumferential ventilation structure is a cylindrical structure with a circular section, a cavity (11) in the cylindrical structure is connected with a fan ventilation opening (6), and a circle of air dispersing openings (7) are arranged on the inner wall of the cylindrical structure;
the multipurpose drying and cutting structure comprises a perforated cylinder (1), an opening is arranged above the perforated cylinder (1), the body of the perforated cylinder (1) is cylindrical, an arc-shaped bottom wall is arranged below the body of the perforated cylinder (1), and a plurality of powder through holes are formed in the body and the bottom wall of the perforated cylinder (1); still contain motor shaft (3) that can rotate in the middle part of taking a hole section of thick bamboo (1), arranged multiunit cutting stirring piece (4) on motor shaft (3), motor (2) are being connected in motor shaft (3).
2. The method for extracting pectin from beet pulp discarded after sugar manufacturing by the two-enzyme method according to claim 1, wherein the drying is air drying until the moisture content is below 8%.
3. The method for extracting pectin from beet pulp discarded after sugar manufacturing by the two-enzyme method according to claim 1, wherein the filter cloth used in the filtering in step (3) is 400 mesh filter cloth.
4. The method for extracting pectin from beet pulp discarded after sugar manufacturing by the two-enzyme method according to claim 1, wherein the plurality of cutting and stirring blades (4) are blades with blades.
5. The method for extracting pectin from sugar beet pulp discarded after sugar production by the two-enzyme method according to claim 1, wherein a material powder storage area (8) is arranged below the circumferential ventilation structure, the side of the material powder storage area (8) is connected with a buffer solution inlet (9), and a mixture outlet pipe (10) is arranged below the material powder storage area (8).
6. The method for extracting pectin from sugar beet pulp discarded after sugar production by the two-enzyme method according to claim 5, wherein a weight sensor is disposed in the material powder storage area (8), and a flow meter is disposed on a pipe of the buffer solution inlet (9).
7. The method for extracting pectin from sugar beet pulp discarded after sugar manufacturing by the two-enzyme method according to claim 5, wherein the mixture outlet pipe (10) is connected to an autoclave.
8. The method for extracting pectin from beet pulp discarded after sugar manufacture by the two-enzyme method according to claim 5, wherein the beet pulp is put into a perforated cylinder, then a ventilation opening of a fan blows, the air is diffused from the cavity through an air diffusing opening and blown to the material in the perforated cylinder for drying, during the drying process, a motor drives a cutting and stirring piece to properly stir, after the drying process, the power of the motor is increased, the cutting and stirring piece is rotated at high speed, the material is crushed to a proper particle size and falls down through a powder through hole of the perforated cylinder to enter a material powder storage area, then a buffer solution is put through a buffer solution inlet, and a mixture outlet pipe enters the next process.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211298443.4A CN115417936B (en) | 2022-10-23 | 2022-10-23 | Method for extracting pectin from waste beet pulp after sugar production by double-enzyme method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211298443.4A CN115417936B (en) | 2022-10-23 | 2022-10-23 | Method for extracting pectin from waste beet pulp after sugar production by double-enzyme method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115417936A CN115417936A (en) | 2022-12-02 |
| CN115417936B true CN115417936B (en) | 2023-04-07 |
Family
ID=84208018
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211298443.4A Active CN115417936B (en) | 2022-10-23 | 2022-10-23 | Method for extracting pectin from waste beet pulp after sugar production by double-enzyme method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115417936B (en) |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103232555A (en) * | 2013-05-16 | 2013-08-07 | 内江师范学院 | Method for extracting pectin from lemon peel residues by utilizing enzymatic method |
| CN103880976A (en) * | 2014-04-02 | 2014-06-25 | 石河子大学 | Microwave-assisted extraction method of beet pectin |
| CN104211831A (en) * | 2014-07-31 | 2014-12-17 | 新疆阜丰生物科技有限公司 | Preparation method of low-methoxyl pectin |
| CN104725527A (en) * | 2013-12-18 | 2015-06-24 | 中粮营养健康研究院有限公司 | Beet pectin and extraction method thereof |
| CN105085706A (en) * | 2014-05-23 | 2015-11-25 | 常州毅博生物科技有限公司 | Method for extracting beet pectin by using protopectinase |
| CN105906741A (en) * | 2016-06-02 | 2016-08-31 | 华南理工大学 | Method for extracting pectin from waste sisal residues |
| CN106800610A (en) * | 2017-01-20 | 2017-06-06 | 杭州垚信生物科技有限公司 | A kind of preparation method of middle gelling beet pectin |
| CN113667034A (en) * | 2021-07-16 | 2021-11-19 | 浙江工业大学 | Method for extracting citrus pectin by using complex enzyme |
-
2022
- 2022-10-23 CN CN202211298443.4A patent/CN115417936B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103232555A (en) * | 2013-05-16 | 2013-08-07 | 内江师范学院 | Method for extracting pectin from lemon peel residues by utilizing enzymatic method |
| CN104725527A (en) * | 2013-12-18 | 2015-06-24 | 中粮营养健康研究院有限公司 | Beet pectin and extraction method thereof |
| CN103880976A (en) * | 2014-04-02 | 2014-06-25 | 石河子大学 | Microwave-assisted extraction method of beet pectin |
| CN105085706A (en) * | 2014-05-23 | 2015-11-25 | 常州毅博生物科技有限公司 | Method for extracting beet pectin by using protopectinase |
| CN104211831A (en) * | 2014-07-31 | 2014-12-17 | 新疆阜丰生物科技有限公司 | Preparation method of low-methoxyl pectin |
| CN105906741A (en) * | 2016-06-02 | 2016-08-31 | 华南理工大学 | Method for extracting pectin from waste sisal residues |
| CN106800610A (en) * | 2017-01-20 | 2017-06-06 | 杭州垚信生物科技有限公司 | A kind of preparation method of middle gelling beet pectin |
| CN113667034A (en) * | 2021-07-16 | 2021-11-19 | 浙江工业大学 | Method for extracting citrus pectin by using complex enzyme |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115417936A (en) | 2022-12-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105942131B (en) | A kind of compound ginger juice clarifying agent and the preparation method for clarifying ginger juice extracting solution | |
| CN104710541A (en) | Method for preparing laminarin from kelp | |
| CN103283931A (en) | Cadmium-eliminated desensitized rice protein, and preparation method and application thereof | |
| CN109198654A (en) | A kind of equipment for extracting dietary fiber from wheat bran | |
| SE461659B (en) | PROCEDURES FOR PREPARING ENZYMET SPS-AS WITH THE ABILITY TO SUBSTITUTE HIGH-MOLECULE CARBON HYDROGEN | |
| CN115417936B (en) | Method for extracting pectin from waste beet pulp after sugar production by double-enzyme method | |
| CN107827996B (en) | Production line and process for extracting green alga proteoglycan from fresh green alga | |
| CN211620489U (en) | Production device for extracting amino acids in sesame seed meal by fermentation method | |
| CN205893218U (en) | Fen -flavor white spirit vertical fermenting equipment | |
| CN112998242A (en) | Preparation device and preparation method of black-bone chicken brewed soy sauce | |
| CN209359530U (en) | A kind of Huanghua pear dark tea pile fermentation machine | |
| CN107692241A (en) | A kind of sweet potato dietary fiber with the slim function of fat reducing and preparation method thereof | |
| CN109619359A (en) | A kind of clarification kiwifruit whole fruit instant powder and preparation method thereof | |
| CN214142386U (en) | Novel extraction, separation and drying integrated equipment | |
| CN107586594A (en) | A kind of peony seed oil extracting method of ethanol homogeneous auxiliary | |
| CN113736615A (en) | Health food rich in SOD and emblic leafflower fruit and its production process | |
| CN109329901A (en) | A kind of lucid ganoderma spirulina tablet of strengthen immunity and preparation method thereof | |
| CN214781753U (en) | Biogas slurry production system for edible mushroom cultivation | |
| CN218530746U (en) | Fruit grain mixing arrangement is used in dairy products processing | |
| CN116649585A (en) | Mulberry deep processing extraction device and extraction process | |
| CN103224544B (en) | Method for preparing rice separation protein by using rice residue | |
| CN101878872B (en) | Broccoli emulsion honey and preparation method thereof | |
| CN217709492U (en) | Slurry mixing and liquefying system for food-grade rice protein | |
| CN117322629B (en) | Preparation method of horseshoe lipid-lowering composition based on fermentation enzymolysis technology | |
| CN214635972U (en) | Homogeneity device of pea starch thick liquid |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |