CN105085706A - Method for extracting beet pectin by using protopectinase - Google Patents
Method for extracting beet pectin by using protopectinase Download PDFInfo
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- CN105085706A CN105085706A CN201410232825.6A CN201410232825A CN105085706A CN 105085706 A CN105085706 A CN 105085706A CN 201410232825 A CN201410232825 A CN 201410232825A CN 105085706 A CN105085706 A CN 105085706A
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Abstract
The invention discloses a method for extracting beet pectin by using protopectinase. The method comprises the following steps: 1, weighing 10kg of 80 mesh beet pulp, adding water with the amount 5-10 times the mass of beet pulp, carrying out immersion washing at 80-90DEG C for 5-15min to remove soluble sugar and impurities and passivate enzymes in the beet pulp, and filtering by using gauze to obtain wet beet pulp; 2, adding 100-300L of a citric acid-sodium citrate buffer solution with the pH value of 4.0-6.0 to the wet beet pulp, adding 1-2.5L of a crude protopectinase solution, and reacting at 40-70DEG C under 150rpm for 2-4h; 3, sequentially respectively carrying out plate and frame filtration and filter core filtration on a pectin extraction liquid to obtain a pectin stock solution, wherein a plate and frame filter has 20 layers of a filter membrane, the filtration precision of the plate and frame filter is 20[mu]m, a filter core filter is a PP folding-20-3 type filter core filter, and the precision of the filter core filter is 0.1[mu]m; 4, carrying out concentrating filtration on the filtered pectin stock solution through using a 8-tube tubular ultrafilter membrane to thoroughly remove impurities and concentrate the pectin stock solution to 3-5%; and 5, drying the concentrated and impurity-removed pectin stock solution through using an experimental high-speed centrifugal spray drier.
Description
Technical field:
The present invention relates to food-processing and process, particularly a kind of method utilizing protopectinase to extract beet pectin.
Background technology:
As everyone knows, pectin substance is a kind of high molecular, electronegative acidic polysaccharose molecule.The main chain of pectin molecule is connected with α-Isosorbide-5-Nitrae-glycosidic link by D-galacturonic acid, and namely so-called " smooth areas ", the carboxyl of the galacturonic acid on main chain has methylating in various degree, and unmethylated carboxyl and K
1+, Na
1+and C
2+combine; Side chain is made up of poly-rhamno-galacturonic acid, and the neutral sugars such as araban, poly-semi-lactosi and poly-arabogalactan are connected with the rhamnosyl in side chain with α-Isosorbide-5-Nitrae-glycosidic link.
Pectin substance extensively exists in the root of higher plant, stem, leaf and fruit, and in beet, apple residue and orange peel, pectin content is the abundantest.The relative molecular mass of the pectin of different sources is all not identical with chemical structure, and the molecular weight of the pectin substance in apple and lemon is 200-369kDa, and the molecular size range of pectin substance contained in orange and beet pulp is but at 40-50kDa.
Beet pulp is that one is rich in the food fibres such as pectin, the byproduct that added value is high, and at present the beet pulp in the whole world is mainly used as feed, only has only a few to utilize sugaring, the case that pectin produced by the beet waste residue of grain distillery.The cheap raw material of the production pectin that beet pulp is that one is rich in pectin (about 15%-30%) and abundance is stablized, can store throughout the year.Current China can produce beet 1,500 ten thousand tons/year, can produce beet pulp about 800,000 tons/year, and beet pulp about contains 25% galacturonic acid (pectin main component) in dry slag, calculates by extraction yield 85%, namely beet pectin 170,000 tons can be produced every year, considerable.Beet pectin molecule formula weight is little, acetyl content height gel force is weak, but but has the emulsifying property that other pectin do not have, and merits attention.
Pectin is the natural additive for foodstuff be widely used in foodstuffs industry, and it is nontoxic, use safety, and pectin is recommended as safe foodstuff additive by foodstuff additive joint committee, and its addition is unrestricted.The annual whole world more than 30000 tons, and keeps the increment of annual approximately 4-5% to the demand of pectin.
At present, a large amount of methods is used for the extraction of pectin, in traditional industry, pectin is obtained from beet pulp, apple residue and orange peel by chemical process, chemical process is traditional acid hydrolyzation, as oxalic acid, hydrochloric acid, nitric acid and sulfuric acid etc., from the angle of economy, chemically extraction pectin is comparatively simple, quick, efficiency is high, and cost is low, but the pectin molecule amount obtained by the method is little, follow-up residue separation difficulty, needs corrosion resistant equipment, meanwhile, produce the pollutent having harm in a large number, cause serious environmental problem; Human consumer more and more hankers after natural food, more and more pays close attention to the chemical additive in food.Therefore, many researchs start the pectin extraction method of a kind of green of steering exploration, under the prerequisite not affecting pectin separating effect, the usage quantity of harmful chemical reagent are dropped to minimum level.
At present, the physical method extracting pectin mainly contains, and supersonic method, autoclaving, milling process and Subcritical Water Extraction method etc., Physical is efficiently fairly simple, but energy consumption is high, is difficult to industrial applications.Enzyme process is carried pectin and be have also been obtained general concern, and it is gentle that enzyme process carries pectin reaction conditions, pollute low, and energy consumption is little, but at present the pectin that obtains of the enzyme process of report of poor quality, extraction yield is low, therefore find a kind of can the enzyme of high efficiency extraction pectin imperative.
Summary of the invention:
In view of above-mentioned technical problem, the invention provides a kind of method utilizing protopectinase to extract beet pectin.The method adopts the protopectinase of different sources to extract pectin, and pectin is not almost degraded, and products obtained therefrom molecular mass is high, and avoids using mineral acid and reducing the application of organic solvent in extraction, reduces environmental pollution.
Concrete technical scheme of the present invention is as follows:
Utilize protopectinase to extract a method for beet pectin, comprise the steps:
A. raw materials pretreatment: take 80 order 10kg beet pulps, add the water of 5 ~ 10 times of beet pulp quality, 80 ~ 90 DEG C of washing by soaking 5 ~ 15 minutes, remove solubility sugar, impurity, itself enzyme in the beet pulp of passivation simultaneously; By filtered through gauze, obtain wet beet pulp;
B. enzymolysis and extraction: the pH value adding 100 ~ 300L in wet beet pulp is 4.0 ~ 6.0 citric acid-sodium citrate buffer, adds 1 ~ 2.5L protopectinase crude enzyme liquid, reacts 2 ~ 4 hours under 40 ~ 70 DEG C of 150rpm conditions;
C. filter: pectin extracting soln, successively respectively through Plate Filtration and filter element filtering, obtains pectin stoste; Plate filter 20 layers of filtering membrane, filtering accuracy is 20 μm, and candle filter is the folding-20-3 type filter cartridge type filter of PP, and filtering accuracy is 0.1 μm;
D. ultrafiltration and concentration: the eight pipe tubular ultra-filtration membranes of the pectin stoste after filtration carry out thickening filtration, thorough removal of impurities, and pectin stoste is concentrated into 3% ~ 5%;
E. dry: the pectin stoste test-type Highspeedcentrifugingandsprayingdrier of concentrating and impurity removing carries out drying.
In such scheme, in described step b, protopectinase crude enzyme liquid is from Paenibacillus polymyxa, enzyme 210U/ml alive.
In such scheme, in described step b, protopectinase crude enzyme liquid is from subtilis, enzyme 150U/ml alive.
In such scheme, in described step b, protopectinase crude enzyme liquid is from Penicillium notatum, enzyme 250U/ml alive.
The whole technique of the inventive method does not use any organic reagent and acid-base material, environmental protection, efficient, and technique is simple, easy to operate.Adopt protopectinase to carry out pectin extraction, have efficient specificity, gained pectin is not almost degraded, and molecular mass is large.
Embodiment:
Make a concrete analysis of below by way of 3 exemplary embodiments and set forth process of the present invention and result, but the treatment condition that the inventive method provides according to scheme are not limited only to following examples.
Embodiment 1:
A. raw materials pretreatment: take 80 order 10kg beet pulps, add the water of 8 times of beet pulp quality, 85 DEG C of washing by soaking 10 minutes, remove solubility sugar, impurity, itself enzyme in the beet pulp of passivation simultaneously.By filtered through gauze, obtain wet beet pulp;
B. enzymolysis and extraction: add 250L citric acid-sodium citrate buffer (pH=4.7) in wet beet pulp, add the protopectinase crude enzyme liquid (enzyme alive 210U/ml) of 2.5L from Paenibacillus polymyxa, react 3 hours under 45 DEG C of 150rpm conditions;
C. filter: pectin extracting soln, successively respectively through Plate Filtration and filter element filtering, obtains pectin stoste; Plate filter 20 layers of filtering membrane, filtering accuracy is 20 μm, and candle filter is the folding-20-3 type filter cartridge type filter of PP, and filtering accuracy is 0.1 μm;
D. ultrafiltration and concentration: the eight pipe tubular ultra-filtration membranes of the pectin stoste after filtration carry out thickening filtration, thorough removal of impurities, and pectin stoste is concentrated into 5%;
E. dry: the pectin stoste test-type Highspeedcentrifugingandsprayingdrier of concentrating and impurity removing carries out drying.
The net result of the present embodiment is: pectin yield is 11.9%, and viscosity-average molecular weight is 47kDa.
Embodiment 2:
A. raw materials pretreatment: take 80 order 10kg beet pulps, add the water of 10 times of beet pulp quality, 85 DEG C of washing by soaking 15 minutes, remove solubility sugar, impurity, itself enzyme in the beet pulp of passivation simultaneously.By filtered through gauze, obtain wet beet pulp;
B. enzymolysis and extraction: add 200L citric acid-sodium citrate buffer (pH=6.0) in wet beet pulp, add the protopectinase crude enzyme liquid (enzyme alive 150U/ml) of 2.0L from subtilis, react 4 hours under 55 DEG C of 150rpm conditions;
C. filter: pectin extracting soln, successively respectively through Plate Filtration and filter element filtering, obtains pectin stoste; Plate filter 20 layers of filtering membrane, filtering accuracy is 20 μm, and candle filter is the folding-20-3 type filter cartridge type filter of PP, and filtering accuracy is 0.1 μm;
D. ultrafiltration and concentration: the eight pipe tubular ultra-filtration membranes of the pectin stoste after filtration carry out thickening filtration, thorough removal of impurities, and pectin stoste is concentrated into 4.5%;
E. dry: the pectin stoste test-type Highspeedcentrifugingandsprayingdrier of concentrating and impurity removing carries out drying.
The net result of the present embodiment is: pectin yield is 23%, and viscosity-average molecular weight is 48kDa.
Embodiment 3:
A. raw materials pretreatment: take 80 order 10kg beet pulps, add the water of 5 ~ 10 times of beet pulp quality, 80 ~ 90 DEG C of washing by soaking 5 ~ 15 minutes, remove solubility sugar, impurity, itself enzyme in the beet pulp of passivation simultaneously.By filtered through gauze, obtain wet beet pulp;
B. enzymolysis and extraction: at wet beet pulp, add 150L citric acid-sodium citrate buffer (pH=6.5), add the protopectinase crude enzyme liquid (enzyme alive 250U/ml) of 1.5L from Penicillium notatum, react 3 hours under 60 DEG C of 150rpm conditions;
C. filter: pectin extracting soln successively, respectively after Plate Filtration and filter element filtering, obtains pectin stoste; Plate filter 20 layers of filtering membrane, filtering accuracy is 20 μm, and candle filter is the folding-20-3 type filter cartridge type filter of PP, and filtering accuracy is 0.1 μm;
D. ultrafiltration and concentration: the eight pipe tubular ultra-filtration membranes of the pectin stoste after filtration carry out thickening filtration, thorough removal of impurities, and pectin stoste is concentrated into 5%;
E. dry: the pectin stoste test-type Highspeedcentrifugingandsprayingdrier of concentrating and impurity removing carries out drying.
The net result of the present embodiment is: pectin yield is 16.6%, and viscosity-average molecular weight is 41kDa.
According to above-described embodiment, protopectinase is the enzyme extracting pectin from plant tissue that can be efficiently special, and non-degradable pectin itself, adopts the crude enzyme liquid of protopectinase to carry out the extraction of pectin in the present invention, have not yet to see the patent extracting pectin with protopectinase;
In addition, adopt the method for ultrafiltration and concentration, can soluble impurity be removed, such as: water colo(u)r, small molecular sugar, small molecular protein etc.; Membrane filter plant also can be utilized simultaneously to complete the concentrated of extracting solution, improve drying efficiency, this technique can reduce floor space, reduce energy consumption, decreasing pollution; Another effect of film device carries out classification to product, utilizes the difference of pectin molecule amount size, by the interception of different magnitude film, beet pectin extracting solution is divided into different grades, with the demand of satisfied different client.
More than show and describe ultimate principle of the present invention and principal character and advantage of the present invention.The technician of the industry should understand; the present invention is not restricted to the described embodiments; what describe in above-described embodiment and specification sheets just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.Application claims protection domain is defined by appending claims and equivalent thereof.
Claims (4)
1. utilize protopectinase to extract a method for beet pectin, comprise the steps:
A. raw materials pretreatment: take 80 order 10kg beet pulps, add the water of 5 ~ 10 times of beet pulp quality, 80 ~ 90 DEG C of washing by soaking 5 ~ 15 minutes, remove solubility sugar, impurity, itself enzyme in the beet pulp of passivation simultaneously; By filtered through gauze, obtain wet beet pulp;
B. enzymolysis and extraction: the pH value adding 100 ~ 300L in wet beet pulp is 4.0 ~ 6.0 citric acid-sodium citrate buffer, adds 1 ~ 2.5L protopectinase crude enzyme liquid, reacts 2 ~ 4 hours under 40 ~ 70 DEG C of 150rpm conditions;
C. filter: pectin extracting soln, successively respectively through Plate Filtration and filter element filtering, obtains pectin stoste; Plate filter 20 layers of filtering membrane, filtering accuracy is 20 μm, and candle filter is the folding-20-3 type filter cartridge type filter of PP, and filtering accuracy is 0.1 μm;
D. ultrafiltration and concentration: the eight pipe tubular ultra-filtration membranes of the pectin stoste after filtration carry out thickening filtration, thorough removal of impurities, and pectin stoste is concentrated into 3% ~ 5%;
E. dry: the pectin stoste test-type Highspeedcentrifugingandsprayingdrier of concentrating and impurity removing carries out drying.
2. the method utilizing protopectinase to extract beet pectin according to claim 1, it is characterized in that, in described step b, protopectinase crude enzyme liquid is from Paenibacillus polymyxa, enzyme 210U/ml alive.
3. the method utilizing protopectinase to extract beet pectin according to claim 1, it is characterized in that, in described step b, protopectinase crude enzyme liquid is from subtilis, enzyme 150U/ml alive.
4. the method utilizing protopectinase to extract beet pectin according to claim 1, it is characterized in that, in described step b, protopectinase crude enzyme liquid is from Penicillium notatum, enzyme 250U/ml alive.
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| CN201410232825.6A CN105085706A (en) | 2014-05-23 | 2014-05-23 | Method for extracting beet pectin by using protopectinase |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105533352A (en) * | 2015-12-17 | 2016-05-04 | 安徽砀山海升果业有限责任公司 | Method for preparing concentrated red beet juice by using multi-enzyme preparation |
| CN106337063A (en) * | 2016-08-26 | 2017-01-18 | 内江师范学院 | Bitter gourd residue comprehensive utilization method |
| RU2658701C1 (en) * | 2017-08-01 | 2018-06-22 | Андрей Михайлович Черников | Method for obtaining a medical pectin |
| CN111253501A (en) * | 2020-04-07 | 2020-06-09 | 武汉轻工大学 | Method for extracting natural pectin from snake skin fruits, natural pectin and application |
| CN111349183A (en) * | 2020-04-22 | 2020-06-30 | 黑龙江八一农垦大学 | Pectin and its extraction method and preparation method |
| CN114634655A (en) * | 2022-03-24 | 2022-06-17 | 重庆檬泰生物科技有限公司 | Pectin production process for coating probiotics |
| CN115417936A (en) * | 2022-10-23 | 2022-12-02 | 华南理工大学 | Method for extracting pectin from waste beet pulp after sugar production by double-enzyme method |
| RU2798564C1 (en) * | 2022-12-15 | 2023-06-23 | федеральное государственное бюджетное образовательное учреждение высшего образования "Уфимский университет науки и технологий" | Method for producing pectin from sugar production waste |
-
2014
- 2014-05-23 CN CN201410232825.6A patent/CN105085706A/en active Pending
Non-Patent Citations (3)
| Title |
|---|
| LEE SEUNG-CHEOL等: "Extraction of pectin from apple pomace with protopectinase produced by Bacillus subtilis IFO 12113", 《HANGUK NONGWHAHAK HOECHI》 * |
| TATSUJI SAKAMOTO等: "Protopectinase-T: a rhamnogalacturonase able to solubilize protopectin from sugar beet", 《CARBOHYDRATE RESEARCH》 * |
| 王姝清等: "从甜菜渣中萃取和净化果胶方法的改进", 《西北林学院学报》 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105533352A (en) * | 2015-12-17 | 2016-05-04 | 安徽砀山海升果业有限责任公司 | Method for preparing concentrated red beet juice by using multi-enzyme preparation |
| CN106337063A (en) * | 2016-08-26 | 2017-01-18 | 内江师范学院 | Bitter gourd residue comprehensive utilization method |
| RU2658701C1 (en) * | 2017-08-01 | 2018-06-22 | Андрей Михайлович Черников | Method for obtaining a medical pectin |
| CN111253501A (en) * | 2020-04-07 | 2020-06-09 | 武汉轻工大学 | Method for extracting natural pectin from snake skin fruits, natural pectin and application |
| CN111349183A (en) * | 2020-04-22 | 2020-06-30 | 黑龙江八一农垦大学 | Pectin and its extraction method and preparation method |
| CN111349183B (en) * | 2020-04-22 | 2022-04-01 | 黑龙江八一农垦大学 | Pectin and its extraction method and preparation method |
| CN114634655A (en) * | 2022-03-24 | 2022-06-17 | 重庆檬泰生物科技有限公司 | Pectin production process for coating probiotics |
| CN115417936A (en) * | 2022-10-23 | 2022-12-02 | 华南理工大学 | Method for extracting pectin from waste beet pulp after sugar production by double-enzyme method |
| CN115417936B (en) * | 2022-10-23 | 2023-04-07 | 华南理工大学 | Method for extracting pectin from waste beet pulp after sugar production by double-enzyme method |
| RU2798564C1 (en) * | 2022-12-15 | 2023-06-23 | федеральное государственное бюджетное образовательное учреждение высшего образования "Уфимский университет науки и технологий" | Method for producing pectin from sugar production waste |
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Application publication date: 20151125 |