CN114634655A - Pectin production process for coating probiotics - Google Patents
Pectin production process for coating probiotics Download PDFInfo
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- CN114634655A CN114634655A CN202210302928.XA CN202210302928A CN114634655A CN 114634655 A CN114634655 A CN 114634655A CN 202210302928 A CN202210302928 A CN 202210302928A CN 114634655 A CN114634655 A CN 114634655A
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- pectin
- water
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- clear water
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- 239000001814 pectin Substances 0.000 title claims abstract description 47
- 235000010987 pectin Nutrition 0.000 title claims abstract description 47
- 229920001277 pectin Polymers 0.000 title claims abstract description 47
- 239000006041 probiotic Substances 0.000 title claims abstract description 26
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 26
- 239000011248 coating agent Substances 0.000 title claims abstract description 21
- 238000000576 coating method Methods 0.000 title claims abstract description 18
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 28
- 230000000529 probiotic effect Effects 0.000 claims abstract description 15
- 238000000034 method Methods 0.000 claims abstract description 13
- 235000005979 Citrus limon Nutrition 0.000 claims abstract description 12
- 238000002156 mixing Methods 0.000 claims abstract description 11
- 238000003756 stirring Methods 0.000 claims abstract description 11
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 claims abstract description 7
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 claims abstract description 7
- 235000011114 ammonium hydroxide Nutrition 0.000 claims abstract description 7
- 229910017604 nitric acid Inorganic materials 0.000 claims abstract description 7
- 230000008569 process Effects 0.000 claims abstract description 7
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 4
- 238000004140 cleaning Methods 0.000 claims abstract description 4
- 239000002245 particle Substances 0.000 claims abstract description 4
- 244000248349 Citrus limon Species 0.000 claims abstract 6
- CDBYLPFSWZWCQE-UHFFFAOYSA-L sodium carbonate Substances [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 20
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims description 11
- 239000000661 sodium alginate Substances 0.000 claims description 11
- 235000010413 sodium alginate Nutrition 0.000 claims description 11
- 229940005550 sodium alginate Drugs 0.000 claims description 11
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 8
- 238000005303 weighing Methods 0.000 claims description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 6
- 238000001556 precipitation Methods 0.000 claims description 6
- 239000007787 solid Substances 0.000 claims description 6
- 238000005507 spraying Methods 0.000 claims description 4
- 108010059820 Polygalacturonase Proteins 0.000 claims description 3
- 238000009835 boiling Methods 0.000 claims description 3
- 108010093305 exopolygalacturonase Proteins 0.000 claims description 3
- 238000007873 sieving Methods 0.000 claims description 3
- 150000001875 compounds Chemical class 0.000 abstract description 3
- 230000002255 enzymatic effect Effects 0.000 abstract description 3
- 238000005469 granulation Methods 0.000 abstract description 3
- 230000003179 granulation Effects 0.000 abstract description 3
- 230000009467 reduction Effects 0.000 abstract description 3
- 239000000243 solution Substances 0.000 description 16
- 241000894006 Bacteria Species 0.000 description 9
- 230000004083 survival effect Effects 0.000 description 9
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 6
- 244000131522 Citrus pyriformis Species 0.000 description 6
- 239000000203 mixture Substances 0.000 description 5
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 4
- 210000004051 gastric juice Anatomy 0.000 description 4
- 230000000968 intestinal effect Effects 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 239000000725 suspension Substances 0.000 description 4
- 240000006024 Lactobacillus plantarum Species 0.000 description 3
- 235000013965 Lactobacillus plantarum Nutrition 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 239000001569 carbon dioxide Substances 0.000 description 3
- 229910002092 carbon dioxide Inorganic materials 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 3
- 238000007710 freezing Methods 0.000 description 3
- 230000008014 freezing Effects 0.000 description 3
- 230000002496 gastric effect Effects 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- 229940072205 lactobacillus plantarum Drugs 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- XKRFYHLGVUSROY-UHFFFAOYSA-N Argon Chemical compound [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 238000003825 pressing Methods 0.000 description 2
- 238000010926 purge Methods 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 239000012488 sample solution Substances 0.000 description 2
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- 229920001202 Inulin Polymers 0.000 description 1
- 239000007836 KH2PO4 Substances 0.000 description 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
- 102000057297 Pepsin A Human genes 0.000 description 1
- 108090000284 Pepsin A Proteins 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 235000004279 alanine Nutrition 0.000 description 1
- 229960003767 alanine Drugs 0.000 description 1
- FYGDTMLNYKFZSV-DZOUCCHMSA-N alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-D-Glcp Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)O[C@H](O[C@@H]2[C@H](OC(O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-DZOUCCHMSA-N 0.000 description 1
- 229910052786 argon Inorganic materials 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000000941 bile Anatomy 0.000 description 1
- 238000007664 blowing Methods 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 235000011148 calcium chloride Nutrition 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 229940099352 cholate Drugs 0.000 description 1
- BHQCQFFYRZLCQQ-OELDTZBJSA-N cholic acid Chemical compound C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 BHQCQFFYRZLCQQ-OELDTZBJSA-N 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 229960002086 dextran Drugs 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- PXEDJBXQKAGXNJ-QTNFYWBSSA-L disodium L-glutamate Chemical compound [Na+].[Na+].[O-]C(=O)[C@@H](N)CCC([O-])=O PXEDJBXQKAGXNJ-QTNFYWBSSA-L 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- FTSSQIKWUOOEGC-RULYVFMPSA-N fructooligosaccharide Chemical compound OC[C@H]1O[C@@](CO)(OC[C@@]2(OC[C@@]3(OC[C@@]4(OC[C@@]5(OC[C@@]6(OC[C@@]7(OC[C@@]8(OC[C@@]9(OC[C@@]%10(OC[C@@]%11(O[C@H]%12O[C@H](CO)[C@@H](O)[C@H](O)[C@H]%12O)O[C@H](CO)[C@@H](O)[C@@H]%11O)O[C@H](CO)[C@@H](O)[C@@H]%10O)O[C@H](CO)[C@@H](O)[C@@H]9O)O[C@H](CO)[C@@H](O)[C@@H]8O)O[C@H](CO)[C@@H](O)[C@@H]7O)O[C@H](CO)[C@@H](O)[C@@H]6O)O[C@H](CO)[C@@H](O)[C@@H]5O)O[C@H](CO)[C@@H](O)[C@@H]4O)O[C@H](CO)[C@@H](O)[C@@H]3O)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@@H](O)[C@@H]1O FTSSQIKWUOOEGC-RULYVFMPSA-N 0.000 description 1
- 229940107187 fructooligosaccharide Drugs 0.000 description 1
- 210000004211 gastric acid Anatomy 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- JYJIGFIDKWBXDU-MNNPPOADSA-N inulin Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@]1(OC[C@]2(OC[C@]3(OC[C@]4(OC[C@]5(OC[C@]6(OC[C@]7(OC[C@]8(OC[C@]9(OC[C@]%10(OC[C@]%11(OC[C@]%12(OC[C@]%13(OC[C@]%14(OC[C@]%15(OC[C@]%16(OC[C@]%17(OC[C@]%18(OC[C@]%19(OC[C@]%20(OC[C@]%21(OC[C@]%22(OC[C@]%23(OC[C@]%24(OC[C@]%25(OC[C@]%26(OC[C@]%27(OC[C@]%28(OC[C@]%29(OC[C@]%30(OC[C@]%31(OC[C@]%32(OC[C@]%33(OC[C@]%34(OC[C@]%35(OC[C@]%36(O[C@@H]%37[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O%37)O)[C@H]([C@H](O)[C@@H](CO)O%36)O)[C@H]([C@H](O)[C@@H](CO)O%35)O)[C@H]([C@H](O)[C@@H](CO)O%34)O)[C@H]([C@H](O)[C@@H](CO)O%33)O)[C@H]([C@H](O)[C@@H](CO)O%32)O)[C@H]([C@H](O)[C@@H](CO)O%31)O)[C@H]([C@H](O)[C@@H](CO)O%30)O)[C@H]([C@H](O)[C@@H](CO)O%29)O)[C@H]([C@H](O)[C@@H](CO)O%28)O)[C@H]([C@H](O)[C@@H](CO)O%27)O)[C@H]([C@H](O)[C@@H](CO)O%26)O)[C@H]([C@H](O)[C@@H](CO)O%25)O)[C@H]([C@H](O)[C@@H](CO)O%24)O)[C@H]([C@H](O)[C@@H](CO)O%23)O)[C@H]([C@H](O)[C@@H](CO)O%22)O)[C@H]([C@H](O)[C@@H](CO)O%21)O)[C@H]([C@H](O)[C@@H](CO)O%20)O)[C@H]([C@H](O)[C@@H](CO)O%19)O)[C@H]([C@H](O)[C@@H](CO)O%18)O)[C@H]([C@H](O)[C@@H](CO)O%17)O)[C@H]([C@H](O)[C@@H](CO)O%16)O)[C@H]([C@H](O)[C@@H](CO)O%15)O)[C@H]([C@H](O)[C@@H](CO)O%14)O)[C@H]([C@H](O)[C@@H](CO)O%13)O)[C@H]([C@H](O)[C@@H](CO)O%12)O)[C@H]([C@H](O)[C@@H](CO)O%11)O)[C@H]([C@H](O)[C@@H](CO)O%10)O)[C@H]([C@H](O)[C@@H](CO)O9)O)[C@H]([C@H](O)[C@@H](CO)O8)O)[C@H]([C@H](O)[C@@H](CO)O7)O)[C@H]([C@H](O)[C@@H](CO)O6)O)[C@H]([C@H](O)[C@@H](CO)O5)O)[C@H]([C@H](O)[C@@H](CO)O4)O)[C@H]([C@H](O)[C@@H](CO)O3)O)[C@H]([C@H](O)[C@@H](CO)O2)O)[C@@H](O)[C@H](O)[C@@H](CO)O1 JYJIGFIDKWBXDU-MNNPPOADSA-N 0.000 description 1
- 229940029339 inulin Drugs 0.000 description 1
- FZWBNHMXJMCXLU-BLAUPYHCSA-N isomaltotriose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@@H](OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O)O1 FZWBNHMXJMCXLU-BLAUPYHCSA-N 0.000 description 1
- 239000000832 lactitol Substances 0.000 description 1
- VQHSOMBJVWLPSR-JVCRWLNRSA-N lactitol Chemical compound OC[C@H](O)[C@@H](O)[C@@H]([C@H](O)CO)O[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O VQHSOMBJVWLPSR-JVCRWLNRSA-N 0.000 description 1
- 229960003451 lactitol Drugs 0.000 description 1
- 235000010448 lactitol Nutrition 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 235000013923 monosodium glutamate Nutrition 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 229940111202 pepsin Drugs 0.000 description 1
- 238000011056 performance test Methods 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 235000020183 skimmed milk Nutrition 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 229960004249 sodium acetate Drugs 0.000 description 1
- 229940073490 sodium glutamate Drugs 0.000 description 1
- 239000000600 sorbitol Substances 0.000 description 1
- 229960002920 sorbitol Drugs 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L5/00—Compositions of polysaccharides or of their derivatives not provided for in groups C08L1/00 or C08L3/00
- C08L5/06—Pectin; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/025—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
- C08K3/00—Use of inorganic substances as compounding ingredients
- C08K3/18—Oxygen-containing compounds, e.g. metal carbonyls
- C08K3/24—Acids; Salts thereof
- C08K3/26—Carbonates; Bicarbonates
- C08K2003/265—Calcium, strontium or barium carbonate
Abstract
The invention discloses a pectin production process for coating probiotics, and relates to the technical field of pectin production. The invention comprises the following steps: the first step is as follows: mixing fresh lemon peel with clear water according to the proportion of 1: 5, mixing in a container, cleaning the fresh lemon peels for 30min, and repeating for 3 times; the second step is that: mixing the washed lemon peel with clear water according to the proportion of 1: 6 is put into a stirring tank to be stirred, the temperature is controlled to be 75-84 ℃, and 65 percent of concentrated nitric acid is added according to 2 per mill of the addition of clear water; the third step: keeping the temperature at 80 ℃, stirring for 2h, and then adding ammonia water with the concentration of 23 percent to adjust the pH value to 3; the fourth step: separating the extractive solution with plate-and-frame filter press, removing residual insoluble particles from the separated extractive solution with diatomite, and vacuum concentrating with experimental MVR evaporator until pectin content is greater than 2.8%. By adopting the enzymatic viscosity reduction and compound granulation modes, the pectin produced by the process has the characteristics of low viscosity, good solubility and good probiotic coating performance.
Description
Technical Field
The invention belongs to the technical field of pectin production, and particularly relates to a production process of pectin for coating probiotics.
Background
Pectin is a high-molecular high-viscosity substance, is difficult to dissolve in water and is often difficult to realize the function development, so various pectin production processes appear in the market; however, pectin produced by the pectin production process in the prior art has high viscosity and poor solubility, and the coating performance of probiotics is generally not ideal.
Disclosure of Invention
The invention aims to provide a pectin production process for coating probiotics, which aims to solve the existing problems that: pectin produced by the pectin production process in the prior art is high in viscosity and poor in solubility, and the coating performance of probiotics is generally not ideal.
In order to solve the technical problems, the invention is realized by the following technical scheme:
the invention relates to a pectin production process for coating probiotics, which comprises the following steps:
the first step is as follows: mixing fresh lemon peel with clear water according to the proportion of 1: 5 in a container, cleaning the fresh lemon peel for 30min, and repeating for 3 times;
the second step is that: mixing the washed lemon peel with clear water according to the proportion of 1: 6 is put into a stirring tank to be stirred, the temperature is controlled to be 75-84 ℃, and 65 percent of concentrated nitric acid is added according to 2 per mill of the addition of clear water;
the third step: keeping the temperature at 80 ℃, stirring for 2h, and then adding ammonia water with the concentration of 23 percent to adjust the pH value to 3;
the fourth step: separating the extractive solution with plate-and-frame filter press, removing residual insoluble particles from the separated extractive solution with diatomite, vacuum concentrating with experimental MVR evaporator until pectin content is more than 2.8%, and maintaining the temperature of the concentrated solution at 40 deg.C;
the fifth step: adjusting pH of the concentrated solution to 3.6 with 23% concentrated ammonia water, adding 500U/mg pectinase (one thirty ten-thousandth of the total amount of the solution), performing enzymolysis for 25min, and adjusting pH to 2 with 65% concentrated nitric acid to stop enzymolysis;
and a sixth step: according to the volume ratio of the enzymolysis liquid to 78% alcohol of 1: 2.2, performing mixed precipitation, separating, and then performing water precipitation for 15min by using 85% alcohol with the same volume, wherein the separated solid is wet pectin, and the solid content is controlled at 78%;
the seventh step: weighing sodium alginate and sodium carbonate, and dissolving in water;
eighth step: spraying the dissolved sodium alginate and sodium carbonate on the surface of the wet pectin, uniformly stirring, drying at 90 ℃ by using a boiling dryer, and crushing after drying until the water content is less than 6%;
the ninth step: and (4) sieving the mixture by a 60-mesh sieve to obtain finished pectin, and using the sieved pectin in a probiotic coating performance test.
Further, the temperature in the second step is controlled at 80 ℃.
Further, the weighed amount of sodium alginate in the seventh step is 0.44% of the weight of the wet pectin, the weighed amount of sodium carbonate is 0.22% of the weight of the wet pectin, and the weighed amount of water is 5% of the weight of the wet pectin.
The invention has the following beneficial effects:
the pectin production process adopts an enzymatic viscosity reduction and compound granulation mode, so that the pectin produced by the process has the characteristics of low viscosity, good solubility and good probiotic coating performance.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention relates to a pectin production process for coating probiotics, which comprises the following steps:
the first step is as follows: mixing fresh lemon peel with clear water according to the proportion of 1: 5 in a container, cleaning the fresh lemon peel for 30min, and repeating for 3 times;
the second step is that: mixing the washed lemon peel with clear water according to the proportion of 1: 6 is put into a stirring tank to be stirred, the temperature is controlled to be 75-84 ℃, in the embodiment, the temperature can be controlled to be 80 ℃, and then 65 percent of concentrated nitric acid is added according to 2 per mill of the addition of clear water;
the third step: keeping the temperature at 80 ℃, stirring for 2h, and then adding ammonia water with the concentration of 23 percent to adjust the pH value to 3;
the fourth step: separating the extractive solution with filter pressing equipment, removing residual insoluble particles from the separated extractive solution with diatomite, vacuum concentrating with experimental MVR evaporator until pectin content is more than 2.8%, and maintaining the temperature of the concentrated solution at 40 deg.C; in the embodiment, in order to effectively separate the extracting solution, the filter pressing equipment adopts a plate-and-frame filter press;
the fifth step: adjusting pH of the concentrated solution to 3.6 with 23% concentrated ammonia water, adding 500U/mg pectinase one-thirty-ten-thousandth of the total amount of the solution, performing enzymolysis for 25min, and adjusting pH to 2 with 65% concentrated nitric acid to stop enzymolysis;
and a sixth step: according to the volume ratio of the enzymolysis liquid to 78% alcohol of 1: 2.2, performing mixed precipitation, separating, and then performing water precipitation for 15min by using 85% alcohol with the same volume, wherein the separated solid is wet pectin, and the solid content is controlled at 78%;
the seventh step: weighing sodium alginate and sodium carbonate, dissolving the sodium alginate and the sodium carbonate in water, wherein the weighing amount of the sodium alginate is 0.44% of the weight of the wet pectin, the weighing amount of the sodium carbonate is 0.22% of the weight of the wet pectin, and the weighing amount of the water is 5% of the weight of the wet pectin;
eighth step: spraying the dissolved sodium alginate and sodium carbonate on the surface of the wet pectin, uniformly stirring, drying at 90 ℃ by using a boiling dryer, and crushing after drying until the water content is less than 6%;
the ninth step: then sieving the pectin with a 60-mesh sieve to obtain the finished pectin.
Finally, the finished pectin after being screened by the 60-mesh sieve is used for testing the probiotic coating performance experiment, and the method comprises the following specific steps:
(1) adding the finished pectin produced by the process into a coating agent to prepare a probiotic suspension;
(2) spraying the probiotic suspension in a high-pressure jet device to form atomized uniform coated liquid drops, and blowing the atomized liquid drops by using frozen airflow to quickly solidify the formed uniformly coated probiotic atomized liquid drops; in the embodiment, the high-pressure air can be one of nitrogen, argon or carbon dioxide, the injection pressure of the high-pressure air is 10-15MPa, and the purging pressure is 1-10 MPa; the purging pressure of the freezing airflow is 1-10MPa, the freezing airflow is specifically freezing carbon dioxide, and the temperature of the adopted carbon dioxide is-40 ℃ to-60 ℃;
(3) and collecting the solidified powder product, and decompressing and heating to normal temperature to obtain the uniformly coated probiotic product.
In the step (1), the concentration of the probiotic suspension is 2-5% (w/w), and the concentration of the coating agent is 30-50% (w/w);
specifically, the coating agent can be one or more of fucoidin, skimmed milk powder, sodium alginate, dextran, inulin, sorbitol, sodium acetate, malto-oligosaccharide, alanine, sodium glutamate, lactitol, vitamin C, calcium chloride, and fructo-oligosaccharide;
in detail, the flow rate of the probiotic suspension liquid is 5-10cm 3/s;
in this embodiment, there are 2 probiotic turbid liquid inlet pipes, and the symmetric distribution.
The probiotic bacteria after coating have gastric acid and bile resistance experiment:
1. gastric juice preparation and manipulation
Weighing a plurality of products with 150 hundred million/g of viable bacteria of the coated lactobacillus plantarum prepared by the process as samples, wherein the weight of each sample is 1g, transferring the samples into a preheated test tube filled with 9mL of simulated gastric juice, and treating the samples for 1h, 2h, 3h and 4h at 37 ℃ and 80r/min respectively, wherein each treatment is repeated for 3 times.
The simulated gastric juice is prepared according to the following method: diluting with 9.5% hydrochloric acid and distilled water to pH 1.5; adding 1.0g of pepsin into each 100mL of the mixture, uniformly mixing the mixture, and filtering the mixture by using a sterile filter membrane of 0.22 mu m, wherein the mixture is used as the preparation;
performing gradient dilution on the treated sample solution and the control strain solution, counting viable bacteria by using a pouring method, and calculating the survival rate, wherein the survival rate is (the number of treated bacteria/the number of original bacteria) multiplied by 100%;
2. preparation and operation of intestinal juice
Weighing a plurality of products with 100 hundred million/g of viable bacteria of the coated lactobacillus plantarum prepared by the process as samples, wherein the weight of each sample is 1g, transferring the samples into a test tube filled with 9mL of simulated intestinal juice, and treating the samples for 1h, 2h, 3h and 4h at 37 ℃ and 80r/min respectively, wherein each treatment is repeated for 3 times;
the simulated intestinal juice is prepared according to the following method: dissolving 6.8g of KH2PO4 in 500mL of distilled water, adding 3g of cholate and 10g of trypsin, adjusting the pH value of the solution to 6.8 by using a NaOH solution with the concentration of 4g/L, fixing the volume to 1L by using the distilled water, mixing uniformly, and filtering by using a sterile filter membrane with the diameter of 0.22 mu m, wherein the preparation is ready for use;
performing gradient dilution on the treated sample solution and the control strain solution, counting viable bacteria by using a pouring method, and calculating the survival rate, wherein the survival rate is (the number of treated bacteria/the number of original bacteria) multiplied by 100%;
TABLE 1 JYBB-163 survival rate data sheet under in vitro simulated gastrointestinal environment
The survival rate data of coated lactobacillus plantarum in an in vitro simulated gastrointestinal environment is shown in table 1, and the survival rate of coated probiotics in an in vitro simulated gastrointestinal environment is very high, 4 hours in gastric juice, the survival rate is still more than 80%, 4 hours in intestinal juice, the survival rate reaches more than 90%, and the functional action is performed to fill up the foundation for the fixed planting of subsequent strains on intestinal tracts.
In conclusion, the pectin production process adopts an enzymatic viscosity reduction and compound granulation mode, so that the obtained pectin has the characteristics of low viscosity, good solubility and good probiotic coating performance.
In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The preferred embodiments of the invention disclosed above are intended to be illustrative only. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise embodiments disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best utilize the invention. The invention is limited only by the claims and their full scope and equivalents.
Claims (3)
1. A production process of pectin for coating probiotics is characterized by comprising the following steps: the method comprises the following steps:
the first step is as follows: mixing fresh lemon peel with clear water according to the proportion of 1: 5 in a container, cleaning the fresh lemon peel for 30min, and repeating for 3 times;
the second step is that: mixing the washed lemon peel with clear water according to the proportion of 1: 6 is put into a stirring tank to be stirred, the temperature is controlled to be 75-84 ℃, and 65 percent of concentrated nitric acid is added according to 2 per mill of the addition of clear water;
the third step: keeping the temperature at 80 ℃, stirring for 2h, and then adding ammonia water with the concentration of 23 percent to adjust the pH value to 3;
the fourth step: separating the extractive solution with plate-and-frame filter press, removing residual insoluble particles from the separated extractive solution with diatomite, vacuum concentrating with experimental MVR evaporator until pectin content is more than 2.8%, and maintaining the temperature of the concentrated solution at 40 deg.C;
the fifth step: adjusting pH of the concentrated solution to 3.6 with 23% concentrated ammonia water, adding 500U/mg pectinase one-thirty-ten-thousandth of the total amount of the solution, performing enzymolysis for 25min, and adjusting pH to 2 with 65% concentrated nitric acid to stop enzymolysis;
and a sixth step: according to the volume ratio of the enzymolysis liquid to 78% alcohol of 1: 2.2, performing mixed precipitation, separating, and then performing water precipitation for 15min by using 85% alcohol with the same volume, wherein the separated solid is wet pectin, and the solid content is controlled at 78%;
the seventh step: weighing sodium alginate and sodium carbonate, and dissolving in water;
eighth step: spraying the dissolved sodium alginate and sodium carbonate on the surface of the wet pectin, uniformly stirring, drying at 90 ℃ by using a boiling dryer, and crushing after drying until the water content is less than 6%;
the ninth step: sieving with 60 mesh sieve to obtain pectin product.
2. The process for the production of pectin for probiotic coating according to claim 1, characterized in that said temperature in said second step is controlled at 80 ℃.
3. The process for producing pectin for probiotic coating according to claim 1, characterized in that said sodium alginate in said seventh step is weighed as 0.44%, said sodium carbonate is weighed as 0.22% and said water is weighed as 5% of the weight of the wet pectin.
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