CN104497100A - Protein separating agent as well as preparation method and application thereof - Google Patents
Protein separating agent as well as preparation method and application thereof Download PDFInfo
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- CN104497100A CN104497100A CN201410817999.9A CN201410817999A CN104497100A CN 104497100 A CN104497100 A CN 104497100A CN 201410817999 A CN201410817999 A CN 201410817999A CN 104497100 A CN104497100 A CN 104497100A
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- wilkinite
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- separating agent
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Abstract
The invention relates to the field of process water treatment and protein recovery in the food processing industry and provides a protein separating agent. The protein separating agent is prepared from the following raw materials: bentonite, sodium alginate, diol gluconate, edible gelatin, water-soluble chitosan and modified starch. The invention also discloses a preparation method of the protein separating agent and application of the protein separating agent in treating food processing process water and recovering soluble protein. The protein separating agent is a composite flocculant and is prepared from all food-grade raw materials, and therefore, the composite flocculant is safe and reliable, good in flocculation effect, high in speed and low in pH influence. The process for recovering the soluble protein from the food processing process water by use of the protein separating agent is a protein cold separation technology which is energy-saving and also capable of increasing the recovery rate of the protein; as a result, more than 98% of protein can be separated and extracted from the process water or waste water.
Description
Technical field
The invention belongs to the process of food-processing industry process water and reclaim albumen field, especially relate to a kind of protein isolation agent and its preparation method and application.
Background technology
Along with improving constantly of living standards of the people, the brewageing of China, food-processing industry fast development, almost there are starch factory, grain distillery in each county, city.Often produce fermentation starch (comprising the self-produced starch of distillery) per year and reach more than 6,000,000 tons.Starch be with starch based cereal or plant roots and stems block as corn, potato class and rice etc. for fermenting raw materials brew forms.During the fermentation, be only that carbohydrate portions is converted into the series product of starch, ethanol and a small amount of ethanol through fermentable, and protein, inorganic salt, crude fat etc. remain in mother liquor.A large amount of nutritive substances is contained as can be seen here in starch wastewater, its nutritive value is roughly suitable with soybean, there is higher recycling be worth, wherein modal comprehensive utilization mode extracts protein from food processing technology water, water after extraction gets back to reuse in technique, realize the zero release of waste water, reach the object of cleaner production.
Summary of the invention
The object of this invention is to provide a kind of protein isolation agent and its preparation method and application.
For realizing above-mentioned goal of the invention, technical scheme of the present invention is as follows:
A kind of protein isolation agent, comprises following feed composition: wilkinite, sodium alginate, gluconic acid two alcohol ester, edible Gelatinum oxhide, water-soluble chitosan and modified starch.
Preferably, the weight percent of described each feed composition is: wilkinite 80 ~ 90%, sodium alginate 2 ~ 5%, gluconic acid two alcohol ester 3 ~ 5%, edible Gelatinum oxhide 1 ~ 3%, water-soluble chitosan 1 ~ 2%, modified starch 1 ~ 5%.
More preferably, the weight percent of described each feed composition is: wilkinite 87%, sodium alginate 3.5%, gluconic acid two alcohol ester 5%, edible Gelatinum oxhide 1.5%, water-soluble chitosan 1%, modified starch 2%.
Described wilkinite, sodium alginate, gluconic acid two alcohol ester, edible Gelatinum oxhide, water-soluble chitosan and modified starch all adopt food grade.
Present invention also offers the preparation method of this protein isolation agent, comprise the steps:
(1) by sodium alginate, gluconic acid two alcohol ester, edible Gelatinum oxhide, water-soluble chitosan and modified starch are ground to 100 orders respectively, mix in proportion;
(2) by wilkinite screening and filtering, the wilkinite after filter is ground to 100 orders again;
(3) above-mentioned wilkinite is dried;
(4) raw material mixed in step (1) is joined in the wilkinite after drying in a certain amount of step (3), fully mix in mixing tank;
(5) the above-mentioned raw material mixed is crossed to filter by 100 object vibratory screening apparatuss be greater than 100 object raw materials, namely obtain protein isolation agent.
Present invention also offers the application of protein isolation agent on process food processing technology Water Sproading soluble proteins.
The principle that protein isolation agent is separated soluble proteins is: soluble protein particulate interface, the potential difference between adsorbed close layer is the current potential of the stern layer of so-called dispersed particle.This protein isolation agent is very easily dissolved in water, charged spherical suede body can be formed rapidly within 5 minutes, and be uniformly distributed in pretreated solution, the attraction of positive and negative charge accelerates the rapid flocculation of soluble protein in solution, break the equilibrium system of original solution, accelerate the bright motion of step between molecule simultaneously, intermolecular collision is aggravated, the small molecules not easily precipitated is made to be combined into macromole, the flocculation of final generation precipitation, makes the soluble protein particulate disperseed occur to assemble rapidly.Its effect is neutralization or the stern layer current potential reducing dispersed particle, both colloidal grain surface was to the position of heterocharge, produce violent adsorption, the micelle diffusion layer of particle surface particle is compressed, the current potential of stern layer reduces, reach the potential barrier reducing close proximity between particle, add the probability of collision between particle, so make soluble protein particulate condense rapidly, form larger throw out, and rapid sedimentation under gravity or mechanical external force effect, reach the object being separated soluble protein.
The advantage that the present invention has and positively effect are: common organic or inorganic flocculation agent has single charge neutrality effect, so just cause its flocculating effect limited.Protein isolation agent in the present invention is a kind of composite flocculation agent, and whole raw material adopts the edible raw material met in GB2760, safe and reliable.Except having the stronger charge neutrality ability of inorganic polymer flocculant and adsorption-flocculation effect, also have the strong adsorption bridging action of organic polymer coargulator, organic and inorganic synergy, flocculating effect is good.In addition, this protein isolation agent also overcomes the problem such as secondary pollution, destruction protein that common organic or inorganic flocculation agent exists.The technique of this protein isolation agent process food processing technology Water Sproading soluble proteins is utilized to be the cold isolation technique of protein, both the energy-conservation rate of recovery turn improving protein, the protein of separable extraction more than 98% from waste water, discharge waste water to discharge after biochemical treatment, its water quality can reach discharging standards.
Embodiment
Below in conjunction with specific embodiment, the invention will be further described, but do not limit protection scope of the present invention.
Embodiment
By sodium alginate, gluconic acid two alcohol ester, edible Gelatinum oxhide, water-soluble chitosan and modified starch are ground to 100 orders respectively, with sodium alginate 3.5%, and gluconic acid two alcohol ester 5%, edible Gelatinum oxhide 1.5%, water-soluble chitosan 1%, the ratio of modified starch 2% mixes; By wilkinite screening and filtering, the wilkinite after filter is again ground to 100 orders and dries; By the above-mentioned sodium alginate mixed in proportion, gluconic acid two alcohol ester, edible Gelatinum oxhide, water-soluble chitosan, modified starch joins in the wilkinite after 87% oven dry, fully mix in mixing tank, then cross to filter by 100 object vibratory screening apparatuss and be greater than 100 object raw materials, namely obtain protein isolation agent.
This protein isolation agent is added in starch wastewater, short mix, solvability albumen in starch wastewater forms flocs unit, the starch wastewater forming flocs unit enters precipitation filling and carries out sedimentation, supernatant liquor removes the original biochemical treatment system of plant area, the albumen of precipitation carries out centrifuge dehydration, namely solids after centrifuge dehydration obtains recovery protein powder further after microwave drying, this technique is the cold isolation technique of protein, both the energy-conservation rate of recovery turn improving protein, the protein of separable extraction more than 98% from waste water, discharge waste water to discharge after biochemical treatment, its water quality can reach discharging standards.
Above embodiments of the invention have been described in detail, but described content being only preferred embodiment of the present invention, can not being considered to for limiting practical range of the present invention.All equalizations done according to the present patent application scope change and improve, and all should still belong within patent covering scope of the present invention.
Claims (6)
1. a protein isolation agent, is characterized in that, comprises following feed composition: wilkinite, sodium alginate, gluconic acid two alcohol ester, edible Gelatinum oxhide, water-soluble chitosan and modified starch.
2. protein isolation agent according to claim 1, is characterized in that, the weight percent of described each feed composition is: wilkinite 80 ~ 90%, sodium alginate 2 ~ 5%, gluconic acid two alcohol ester 3 ~ 5%, edible Gelatinum oxhide 1 ~ 3%, water-soluble chitosan 1 ~ 2%, modified starch 1 ~ 5%.
3. protein isolation agent according to claim 2, is characterized in that, the weight percent of described each feed composition is: wilkinite 87%, sodium alginate 3.5%, gluconic acid two alcohol ester 5%, edible Gelatinum oxhide 1.5%, water-soluble chitosan 1%, modified starch 2%.
4. the protein isolation agent according to any one of claim 1-3, is characterized in that, described wilkinite, sodium alginate, gluconic acid two alcohol ester, and edible Gelatinum oxhide, water-soluble chitosan and modified starch all adopt food grade.
5. a preparation method for protein isolation agent according to claim 4, is characterized in that, comprises the steps:
(1) by sodium alginate, gluconic acid two alcohol ester, edible Gelatinum oxhide, water-soluble chitosan and modified starch are ground to 100 orders respectively, mix in proportion;
(2) by wilkinite screening and filtering, the wilkinite after filter is ground to 100 orders;
(3) above-mentioned wilkinite is dried;
(4) raw material mixed in step (1) is joined in the wilkinite after drying in a certain amount of step (3), fully mix in mixing tank;
(5) the above-mentioned raw material mixed is crossed to filter by 100 object vibratory screening apparatuss be less than 100 object raw materials, namely obtain protein isolation agent.
6. the application of protein isolation agent according to claim 1 on process food processing technology Water Sproading soluble proteins.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410817999.9A CN104497100A (en) | 2014-12-24 | 2014-12-24 | Protein separating agent as well as preparation method and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410817999.9A CN104497100A (en) | 2014-12-24 | 2014-12-24 | Protein separating agent as well as preparation method and application thereof |
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| CN104497100A true CN104497100A (en) | 2015-04-08 |
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| CN201410817999.9A Pending CN104497100A (en) | 2014-12-24 | 2014-12-24 | Protein separating agent as well as preparation method and application thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106262764A (en) * | 2016-08-17 | 2017-01-04 | 武汉正天成生物科技有限公司 | Natural clarifying agent and application thereof |
| CN110218239A (en) * | 2019-06-18 | 2019-09-10 | 安徽省环境科学研究院 | A kind of method and application recycling Sweet potato starch wastewater albumen |
| CN110841604A (en) * | 2019-09-26 | 2020-02-28 | 西安交通大学 | Copper ion chelated carboxyl functionalized magnetic bentonite and preparation method and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1693483A (en) * | 2005-06-14 | 2005-11-09 | 福建师范大学 | Method of clarifying saccharic crop pressed juice mixture by using bentonite-chitin composite flocculant |
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2014
- 2014-12-24 CN CN201410817999.9A patent/CN104497100A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1693483A (en) * | 2005-06-14 | 2005-11-09 | 福建师范大学 | Method of clarifying saccharic crop pressed juice mixture by using bentonite-chitin composite flocculant |
Non-Patent Citations (3)
| Title |
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| 李妮妮: "壳聚糖可食性复合薄膜的性能", 《广东工业大学学报》 * |
| 李永明: "壳聚糖絮凝剂在水处理中的应用研究进展", 《水处理技术》 * |
| 李芳蓉: "水处理絮凝剂的应用现状及发展趋势", 《甘肃科技》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106262764A (en) * | 2016-08-17 | 2017-01-04 | 武汉正天成生物科技有限公司 | Natural clarifying agent and application thereof |
| CN106262764B (en) * | 2016-08-17 | 2020-02-11 | 武汉正天成生物科技有限公司 | Natural clarifying agent and application thereof |
| CN110218239A (en) * | 2019-06-18 | 2019-09-10 | 安徽省环境科学研究院 | A kind of method and application recycling Sweet potato starch wastewater albumen |
| CN110841604A (en) * | 2019-09-26 | 2020-02-28 | 西安交通大学 | Copper ion chelated carboxyl functionalized magnetic bentonite and preparation method and application thereof |
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