CN102180980A - Method for producing rice starch by adopting enzyme method and product obtained by using method - Google Patents
Method for producing rice starch by adopting enzyme method and product obtained by using method Download PDFInfo
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Abstract
The invention relates to a method for improving the extraction rate of rice starch produced by using an enzyme method. The method comprises the steps of broken rice pretreatment, pulping, enzyme reaction, cyclone washing, dehydration, air flow drying and the like. The invention also relates to the rice starch obtained by the method. The starch granules are small, the starch has uniform granularity (2 to 8 microns) and complete structure, the protein content is less than or equal to 0.35 percent, the quality of the starch reaches American food and drug administration (FDA) standards, the extraction rate of the starch is more than or equal to 90 percent, and the starch can be widely applied in the industries of cosmetics, pharmacy, food, chemical industry and the like.
Description
[technical field]
The invention belongs to rice intensive processing technical field.More specifically, invention relates to a kind of method that adopts the Production by Enzymes Starch rice, also relates to the Starch rice for preparing according to described method.
[background technology]
Starch rice is the major ingredient in cracking rice, and the form with starch granules in rice exists.In the middle of all known cereal, the rice starch granule minimum, particle diameter is about 2 μ m-8 μ m, and its shape majority is irregular polygon, and corner angle are remarkable.Starch rice suction after the gelatinization is fast, and the matter structure is soft and smooth like cream, has the mouthfeel of fat.Based on these characteristics of Starch rice, it can be used as powder and paper grade (stock) glue, lubricant, the sugar-coat of candy and the vehicle of tablet etc. that makeup are applied powder, developing out paper is used.Therefore, Starch rice is widely used in industries such as food, pharmacy, chemical industry with its distinctive character, and huge market potential is arranged.
It is crucial removing albumen in the process of extracting starch, protein content≤0.5% in the U.S. FDA standard-required starch.The compact internal structure of rice endosperm, starch granules is tiny, and almost all exist with the composite grain form, protein combines closely with the starch granules envelope, it more than the former 80% the very big gluten of molecular weight in addition, intermolecularly undertaken crosslinkedly being condensed into fine and close nettedly by disulfide linkage and hydrophobic grouping, therefore compare with other cereal, the removal of rice protein is difficulty comparatively.
Starch extracting method commonly used both at home and abroad mainly contains alkaline leaching and enzyme process.Alkaline leaching is to soak rice with dilute alkaline soln earlier, remove soak solution after the immersion, add new alkali lye wet-milling, put into settling bath then and carry out size classification by the precipitator method, remove top clarification part, with the starch emulsion washed through the centrifuge dehydration separating starch, drying and crushing gets product, but under the high alkalinity condition, the gained starch structure is easily impaired, protein is residual higher, the too high starch of protein content is apt to deteriorate, protein can and starch in the glucose generation non-enzymatic browning that transforms, influence quality product and outward appearance, and this method production cycle is long, the cost height has caused pollution to environment in large-scale industrial production.Enzyme process is that the effect by proteolytic enzyme makes proteolysis, thereby is easy to separate with starch, and this method does not have destructiveness, production cost low, pollution-free to Starch rice.But existing research adopts combined-enzyme method protolysate to produce in the process of Starch rice, because rice starch granule minimum, and molecular weight of albumen is bigger, the small-particle proportion of latter part of proteic big fragment of hydrolysis and partial starch is more approaching, when cyclonic separation, starch can not effectively separate with albumen, in order to reduce the protein content in the starch, obtain quality Starch rice preferably, will remove albumen as much as possible, will carry a certain amount of starch secretly in the isolating albumen, make the extraction yield of starch reduce.
CN200310112702.0 discloses a kind of Enzymatic Production Technology Of Rice Starch, has used β proteolytic enzyme in the method, and when the protein content in the product drops to 0.3% when following, starch yield reaches more than 55%.
CN200610010960.1 discloses a kind of method of utilizing prozyme to produce rice starch and rice gluten polypeptide powder, Sumizyme MP, aspartic protease, neutral protease and flavor protease have wherein been used, protein content in the rice starch that obtains is lower than 0.5%, and starch yield is more than 90%.
In order to solve the disadvantage of prior art, the inventor is through test of many times, and the present invention has been finished in research.
[summary of the invention]
[technical problem that will solve]
The purpose of this invention is to provide a kind of method that improves Production by Enzymes Starch rice extraction yield.
Another object of the present invention provides the Starch rice that obtains according to described method.
[technical scheme]
The present invention is achieved through the following technical solutions.
The present invention relates to a kind of method that adopts the Production by Enzymes Starch rice.Described method comprises the steps:
(1) pre-treatment of cracking rice
According to the weight ratio 1 of cracking rice with water: 1-2, crack rice once with water rinse, discard washing water of rice, with the water logging bubble of the same amount 2-3h that cracks rice, pulverize then again, slurry obtains cracking rice;
(2) defibrination
Step (1) obtains, and the slurry of cracking rice grinds to form Rice ﹠ peanut milk with colloidal mill, add then entry with the concentration adjustment of Rice ﹠ peanut milk to 7-8 ° of B é, with aqueous sodium hydroxide solution its pH value is adjusted to 8.5-9.0 again;
(3) enzyme reaction
The Rice ﹠ peanut milk that step (2) is obtained is heated to temperature 50-60 ℃, add then in the quality 0.4-0.6% Sumizyme MP of cracking rice, stir, carry out enzyme digestion reaction 1.5-2.5h, add again in the quality 0.4-0.6% neutral protease of cracking rice, continue enzyme digestion reaction 2.5-3.5h, thereby obtain a kind of enzymolysis solution;
(4) eddy flow washing
The enzymolysis solution that step (3) is obtained is delivered to cyclone separator and is carried out separating, washing and concentrate, and reclaims eddy flow washing heavy phase and starch milk;
(5) dehydration
With described eddy flow washing heavy phase and described starch milk mixing, by the filter press dehydration, make the moisture weight percentage in its material be lower than 50% then;
(6) air stream drying
The material that step (5) obtains carries out drying by the pulsed gas flow drying installation again, obtains described Starch rice.
A preferred embodiment of the invention, the fineness of pulverizing of cracking rice in the step (1) is 60 orders.
According to another kind of preferred implementation of the present invention, the Rice ﹠ peanut milk after colloidal mill grinds in the step (2) is by 80 mesh sieves.
According to another kind of preferred implementation of the present invention, the concentration expressed in percentage by weight of aqueous sodium hydroxide solution is 35-45% described in the step (2).
According to another kind of preferred implementation of the present invention, in the step (3), the Rice ﹠ peanut milk that step (2) obtains is heated to temperature 50-55 ℃.
According to another kind of preferred implementation of the present invention, the enzyme work of Sumizyme MP is 200,000 IU/g; The enzyme work of neutral protease is 130,000 IU/g.
According to another kind of preferred implementation of the present invention, described cyclone separator is 18 grades of cyclone separators, and wherein 9 grades are separated washing, 3 grades of concentrated and 6 grades of secondary separations washings.
According to another kind of preferred implementation of the present invention, the washing water yield of eddy flow washing counts 1 with starch weight in the step (4): 2-2.5, swirler feed pressure 0.45-0.6MPa, oil pressure relief 0.2-0.3MPa, wake flow pressure 0.1-0.2MPa.
According to another kind of preferred implementation of the present invention, the inlet temperature of pulsed gas flow drying installation is 130-150 ℃ in the step (6), and temperature out is lower than 60 ℃.
The invention still further relates to a kind of Starch rice for preparing according to described method, described rice starch granule granularity is 2-8 μ m, structural integrity, albumen weight percentage≤0.35% wherein.
Below the present invention will be described in more detail.
(1) pre-treatment of cracking rice
According to cracking rice and water weight ratio 1: 1-2, crack rice once with water rinse, discard washing water of rice, steep the 2-3h that cracks rice with the water logging of same amount again, to pulverize then, fineness reaches 60 orders, obtains Rice ﹠ peanut milk.
The raw material range that the present invention uses is wider, both can use the finished product rice, also can use and crack rice, and the no sandstone that requires to crack rice, metallic impurity, nothing are gone mouldy, no insect pest.
Crack rice and carry out rinsing, immersion and coarse reduction in advance, make the ground rice fineness reach 60 orders.
Pulverizer and screening plant all are that use usually in the present technique field, product sold in the market.
(2) defibrination
With colloidal mill the Rice ﹠ peanut milk that step (1) obtains is ground to form Rice ﹠ peanut milk, Rice ﹠ peanut milk after the grinding adds entry then by 80 mesh sieves, with the concentration adjustment of Rice ﹠ peanut milk to 7-8 ° of B é, add the aqueous sodium hydroxide solution that concentration expressed in percentage by weight is 35-45% then, with its pH regulator to 8.5-9.0.
The ultimate principle of colloidal mill be fluid or semi-fluid material by at a high speed relatively interlock decide be subjected to effects such as powerful shearing force, friction and high-frequency vibration between tooth and the moving tooth, material is disperseed effectively, floatingization, pulverizing, homogeneous.The colloidal mill that the present invention uses is that adopt usually in the present technique field, product sold in the market, the JML series colloidal mill that for example can use Wenzhou City company of the imperial colloidal mill of person of outstanding talent factory to sell.
In order to extract Starch rice and convenient preparation wherein as much as possible, suitable rice slag particle degree is to be lower than 80 sieve meshes.The sieve that can adopt in the market the those skilled in the art that sell to use always screens.
The present invention uses aqueous sodium hydroxide solution to regulate the pH value of Rice ﹠ peanut milk.The concentration expressed in percentage by weight of aqueous sodium hydroxide solution is 35-45%.When the concentration expressed in percentage by weight of aqueous sodium hydroxide solution during greater than 35-45%, can cause local alkali overrich, destroy the starch granules structure, albumen forms gel; When the concentration expressed in percentage by weight of aqueous sodium hydroxide solution during less than 35-45%, can cause and add alkali lye simultaneously, alkali lye and albumen constantly react, the increasing of alkali lye consumption.
Because the pH value of Rice ﹠ peanut milk will influence follow-up enzyme digestion reaction, therefore, the pH value of described feed liquid is adjusted to 8.5-9.0 is advisable.If it is shorter that the pH value of described feed liquid less than 8.5, then can cause the required alkaline condition of alkaline enzyme effect to hold time, the reduction alive of alkaline enzyme is too fast; If the pH value of described feed liquid greater than 9.0, then can cause starch granules destructurized.
(3) enzyme reaction
With 50-60 ℃ of the Rice ﹠ peanut milk Heating temperature behind step (2) the adjusting pH, preferably 50-55 ℃, in described Rice ﹠ peanut milk, add then in the quality 0.4-0.6% Sumizyme MP of cracking rice, stir, carry out enzyme digestion reaction 1.5-2.5h, add again in the quality 0.4-0.6% neutral protease of cracking rice, carry out enzyme digestion reaction 2.5-3.5h, thereby obtain a kind of enzymolysis solution.
Sumizyme MP is to make the 2709 withered grass bar microorganisms that educate through bacterium protoplastis mutafacient system to reach the refining a kind of proteolytic ferment that forms by submerged fermentation, extraction, belong to the crisp outer high alkaline proteases of a kind of Serine, it can generate polypeptide or amino acid by protein hydrolysate molecule peptide chain, has the ability of stronger decomposing protein.It is suitable for using under 40-55 ℃, the alkaline condition of pH9-11, and the vigor that exceeds above scope enzyme descends.
Enzyme activity is defined as 1 gram enzyme powder or 1ml enzyme liquid under 40 ℃ of conditions with pH4.6, and it is 1 enzyme work unit that 1 hour decomposing soluble starch produces the enzyme amount of 1mg glucose.
The enzyme work of the Sumizyme MP of Shi Yonging in the present invention is 50,000～1,500,000 IU/g, preferably 200,000 IU/g.Its addition is in the quality 0.4-0.6% that cracks rice.
The Sumizyme MP that the present invention uses for example is the Sumizyme MP that Novozymes Company produces.
In the present invention, in Rice ﹠ peanut milk, add, when the addition of Sumizyme MP is lower than this scope, can cause proteolysis insufficient, can not effectively separate with starch in the quality 0.4-0.6% Sumizyme MP of cracking rice; When the addition of Sumizyme MP was higher than this scope, it was too fast to cause material liquid pH to descend, and enzyme work can not play one's part to the full, and causes the waste of enzyme.
After adding described Sumizyme MP, under temperature 50-60 ℃, carry out enzyme digestion reaction.The temperature condition of enzyme digestion reaction is most important, if be lower than this temperature range, can reduce enzyme and live; If be higher than this temperature range, then can the enzyme denaturation inactivation, starch pasting.
Neutral protease is got through fermented extracted by subtilis, belongs to a kind of restriction endonuclease, can be used for the range protein hydrolysis treatment.Under certain temperature, pH value, neutral protease can be hydrolyzed to macro-molecular protein products such as amino acid.Its suitable hydrolysis temperature is 35-55 ℃, and the appropriate pH value is 6.0-7.5.
The enzyme work of the neutral protease of Shi Yonging in the present invention is 50,000～300,000 IU/g, preferably 130,000 IU/g.Its addition is in the quality 0.4-0.6% that cracks rice.
The neutral protease that the present invention uses for example is the neutral protease that Novozymes Company produces.
In the present invention, in Rice ﹠ peanut milk, add, when the addition of neutral proteolytic enzyme is lower than this scope, can cause proteolysis insufficient, can not effectively separate with starch in the quality 0.4-0.6% neutral protease of cracking rice; When the addition of neutral proteolytic enzyme is higher than this scope, material liquid pH is reduced too much, near most of proteic iso-electric points, cause dissolved albumen to wait again and a little separate out, be unfavorable for separating with starch.
After adding described neutral protease, under temperature 50-60 ℃, carry out enzyme digestion reaction.The temperature condition of enzyme digestion reaction is most important, if be lower than this temperature range, can reduce enzyme and live; If be higher than this temperature range, then can the enzyme denaturation inactivation, starch pasting.
(4) eddy flow washing
The enzymolysis solution that step (3) is obtained is delivered to cyclone separator and is carried out separating, washing and concentrate, and reclaims eddy flow washing heavy phase and starch milk.
Swirler is that the liquid cyclone pipe by many minor diameters combines, and is more extensive at starch industry and close sector application.The Rice ﹠ peanut milk that contains starch enters from certain level, in turn by at different levels.From the discharging of last step underflow, wash water from the last step fresh feed pump enter, adverse current is discharged by first step overflow by until previous stage successively.The purpose of this device is further to remove to separate after husband's matter solvable and insoluble impurity in the starch milk, and these impurity mainly are protein.Insoluble protein is the part of husband's matter, and soluble impurity comprises soluble protein and inorganic salt one class impurity.
The washing water yield of eddy flow washing is in starch weight 1: 2-2.5, swirler feed pressure 0.45-0.6MPa, oil pressure relief 0.2-0.3MPa, wake flow pressure 0.1-0.2MPa.
18 grades of cyclone separators that the present invention uses are product solds on the market, for example are the products of being produced by the auspicious general mechanical company limited in Wuxi.18 grades of cyclone separators that the present invention uses, wherein 9 grades are separated washing, 3 grades of light phases of concentrated higher level's eddy flow washing gained, eddy flow washing heavy phase and starch milk are reclaimed in 6 grades of secondary separation washings.
(5) dehydration
With described eddy flow washing heavy phase and described starch milk mixing, by the filter press dehydration, make the moisture weight percentage in its material be lower than 50% then.
Described filter press dehydration is a kind of employing plate-and-frame filter press dehydration method, the plate-and-frame filter press that the present invention uses is the various plate-and-frame filter press of selling in the market, and for example company of Hangzhou Beit Filter Co., Ltd is with the plate-and-frame filter press of trade(brand)name XAM1500-UBK template paper blotter press sale.
(6) air stream drying
By setting pulse formula Pneumatic drying device, make its inlet temperature at 130-150 ℃, temperature out is below 60 ℃, and the material that step (5) obtains is further dry, obtains described Starch rice.
Air stream drying claims wink-dry again.Be from the small-particle that is easy to dewater, Powdered wet stock, to remove moisture content (mainly being surface water) rapidly.In the air stream drying,, make the quality behind the product drying obtain best the assurance because residence time of material is extremely short.
The pulsed pneumatic drying device that the present invention uses can be the pulsed pneumatic drying device produced with trade(brand)name QG series of pulses pneumatic drier of Jiangyin golden hair drying plant company limited for example.
In the present invention, the method for calculation of Starch rice extraction yield and residual protein content are as follows:
Pure Starch rice * 100% in pure Starch rice/raw material that Starch rice extraction yield (%)=extraction obtains
Therefore adopt method of the present invention, the Starch rice extraction yield can reach more than 90%, and the Starch rice extraction yield that prior art can reach is 70%, the Starch rice extraction yield of the present invention Starch rice extraction yield that can reach far above prior art.
Albumen weight/product weight (butt) * 100% in the weight percentage of residual protein (%)=goods
Adopt method of the present invention, the weight percentage of residual protein≤0.35%, quality product reaches the U.S. FDA standard.
In the present invention, adopt conventional laser particle size analysis method to analyze the granularity of Starch rice of the present invention, analytical results is seen accompanying drawing 1.
The Starch rice that obtains prepared according to the methods of the invention can be widely used in industries such as makeup, pharmacy, food, papermaking, chemical industry.
[beneficial effect]
Compared with prior art, the inventive method has following advantage: adopt combined-enzyme method protolysate, by controlling proteic hydrolysis degree, destroy the network structure between starch and the albumen, most of proteolysis can be become the polypeptide of solubility etc., removed by solid-liquid separation, increase the difference in specific gravity between undissolved protein fragments and the starch granule simultaneously, earlier with the feed liquid behind 9 grades of eddy flow washing separation enzymolysis, the heavy phase that obtains meets target level of product quality, light concentrating through 3 grades mutually reaches 6 grades of separation washings, adjusting by the eddy flow parameter, increase the separation force between undissolved protein fragments and the small molecules starch, reclaim the starch that albumen is carried secretly, with the heavy phase combination drying, make the gained starch granules little, homogeneous grain diameter (2-8 μ m), structural integrity, protein content in the product is less than 0.35%, quality reaches U.S. FDA standard (the FDA standard is that protein content is less than 0.5%), the extraction yield of starch is greater than 90%, existing Starch rice extractive technique has raising by a relatively large margin, and, having alleviated the bitter taste of hydrolyzed solution by control to protein degree, the protein after the hydrolysis can also further be used to produce the rice protein peptide.
[description of drawings]
Accompanying drawing 1 is a Starch rice particle size distribution figure (normal distribution scope 2-8 μ m) prepared according to the methods of the invention.
[embodiment]
Embodiment 1:The method of raising Production by Enzymes Starch rice extraction yield of the present invention
The step of this method is as follows:
(1) pre-treatment of cracking rice
Take by weighing early rice 500kg (starch content 76.5%), be delivered to steeping tank through vibratory screening apparatus, tramp iron separator, chapelet, according to cracking rice and water weight ratio 1: 1, crack rice once with water rinse, discard washing water of rice, steep the 2.5h that cracks rice with the water logging of same amount again, the raw material after soaking is delivered to hammer crusher by the stainless steel conveying belt carries out coarse reduction to 60 order, obtain Rice ﹠ peanut milk.
(2) defibrination
Slurries input colloidal mill after pulverizing is ground, Rice ﹠ peanut milk after the grinding adds entry then by 80 order centrifugal screens, and the concentration expressed in percentage by weight of Rice ﹠ peanut milk is adjusted to 8 ° of B é, add concentration expressed in percentage by weight then and be 40% aqueous sodium hydroxide solution, with Rice ﹠ peanut milk pH regulator to 8.5.
(3) enzyme reaction
With 52 ℃ of the Rice ﹠ peanut milk Heating temperatures behind step (2) the adjusting pH, in described Rice ﹠ peanut milk, add the Sumizyme MP of producing in quality 0.6% Novozymes Company of cracking rice (enzyme lives 200,000) then, stir, carry out enzyme digestion reaction 2h, add the neutral protease of producing in quality 0.6% Novozymes Company of cracking rice (enzyme lives 130,000) again, carry out enzyme digestion reaction 2.5h, thereby obtain enzymolysis solution.
(4) eddy flow washing
After enzyme digestion reaction finishes, the enzymolysis solution of step (3) is squeezed into 18 grades of cyclone separators (the auspicious general mechanical company limited in Wuxi), wherein 9 grades are separated washing, the washing water yield is counted 1: 2.3 with starch weight, swirler feed pressure 0.6MPa, overflow 0.25MPa, wake flow 0.1MPa, 3 grades of light phases of concentrated higher level's eddy flow washing gained, the starch in the concentrated solution is reclaimed in 6 grades of secondary separation washings.
(5) dehydration
With the heavy phase and the recovery gained starch milk mixing of twice eddy flow washing gained, by the plate-and-frame filter press dehydration that Hangzhou Beit Filter Co., Ltd produces, remove moisture wherein, make moisture content of material be lower than 50% concentration expressed in percentage by weight;
(6) air stream drying
Pulsed gas flow drying installation by the production of control Jiangyin golden hair drying plant company limited, make its inlet temperature at 132 ℃, temperature out is below 60 ℃, the material that step (5) obtains is further dry, obtain described Starch rice 428kg, adopt the methods analyst of describing in this specification sheets to obtain: starch content 80.8%, residual protein content 0.35%, extraction yield 90.5%.
Embodiment 2:The method of raising Production by Enzymes Starch rice extraction yield of the present invention
The step of this method is as follows:
(1) pre-treatment of cracking rice
Take by weighing early rice 600kg (starch content 77.4%), be delivered to steeping tank through vibratory screening apparatus, tramp iron separator, chapelet, according to cracking rice and water weight ratio 1: 1.5, crack rice once with water rinse, discard washing water of rice, steep the 2h that cracks rice with the water logging of same amount again, the raw material after soaking is delivered to hammer crusher by the stainless steel conveying belt carries out coarse reduction to 60 order, obtain Rice ﹠ peanut milk.
(2) defibrination
The colloidal mill that company of the imperial colloidal mill of the slurries input person of outstanding talent of Wenzhou City factory after pulverizing is produced grinds, Rice ﹠ peanut milk after the grinding is by 80 order centrifugal screens, add entry then, the concentration expressed in percentage by weight of Rice ﹠ peanut milk is adjusted to 7.5 ° of B é, add concentration expressed in percentage by weight then and be 45% aqueous sodium hydroxide solution, with Rice ﹠ peanut milk pH regulator to 8.7.
(3) enzyme reaction
With 50 ℃ of the Rice ﹠ peanut milk Heating temperatures behind step (2) the adjusting pH, in Rice ﹠ peanut milk, add the Sumizyme MP of producing in the quality 0.5%. Novozymes Company of cracking rice (enzyme lives 200,000) then, stir, carry out enzyme digestion reaction 2.5h, add the neutral protease of producing in quality 0.5% Novozymes Company of cracking rice (enzyme lives 130,000) again, carry out enzyme digestion reaction 3h, thereby obtain enzymolysis solution.
(4) eddy flow washing
After enzyme digestion reaction finishes, the enzymolysis solution of step (3) is squeezed into 18 grades of cyclone separators (the auspicious general mechanical company limited in Wuxi), wherein 9 grades are separated washing, the washing water yield is counted 1: 2 with starch weight, swirler feed pressure 0.52MPa, overflow 0.3MPa, wake flow 0.15MPa, 3 grades of light phases of concentrated higher level's eddy flow washing gained, the starch in the concentrated solution is reclaimed in 6 grades of secondary separation washings.
(5) dehydration
With the heavy phase and the recovery gained starch milk mixing of twice eddy flow washing gained, by the plate-and-frame filter press dehydration that Hangzhou Beit Filter Co., Ltd produces, remove moisture wherein, make moisture content of material be lower than 50% concentration expressed in percentage by weight;
(6) air stream drying
Pulsed gas flow drying installation by the production of control Jiangyin golden hair drying plant company limited, make its inlet temperature at 130 ℃, temperature out is below 60 ℃, the material that step (5) obtains is further dry, obtain described Starch rice 535kg, adopt the methods analyst of describing in this specification sheets to obtain: starch content 79.0%, residual protein content 0.40%, yield 91.0%.
Embodiment 3:The method of raising Production by Enzymes Starch rice extraction yield of the present invention
The step of this method is as follows:
(1) pre-treatment of cracking rice
Take by weighing early rice 800kg (starch content 76.1%), be delivered to steeping tank through vibratory screening apparatus, tramp iron separator, chapelet, according to cracking rice and water weight ratio 1: 2, crack rice once with water rinse, discard washing water of rice, steep the 3h that cracks rice with the water logging of same amount again, the raw material after soaking is delivered to hammer crusher by the stainless steel conveying belt carries out coarse reduction to 60 order, obtain Rice ﹠ peanut milk.
(2) defibrination
Slurries input colloidal mill after pulverizing is ground, Rice ﹠ peanut milk after the grinding adds entry then by 80 order centrifugal screens, and the concentration expressed in percentage by weight of Rice ﹠ peanut milk is adjusted to 7 ° of B é, add concentration expressed in percentage by weight then and be 35% aqueous sodium hydroxide solution, with Rice ﹠ peanut milk pH regulator to 9.0.
(3) enzyme reaction
With 55 ℃ of the Rice ﹠ peanut milk Heating temperatures behind step (2) the adjusting pH, in Rice ﹠ peanut milk, add the Sumizyme MP of producing in quality 0.4% Novozymes Company of cracking rice (enzyme lives 200,000) then, stir, carry out enzyme digestion reaction 1.5h, add the neutral protease of producing in quality 0.4% Novozymes Company of cracking rice (enzyme lives 130,000) again, carry out enzyme digestion reaction 3.5h, thereby obtain enzymolysis solution.
(4) eddy flow washing
After enzyme digestion reaction finishes, the enzymolysis solution of step (3) is squeezed into 18 grades of cyclone separators (the auspicious general mechanical company limited in Wuxi), wherein 9 grades are separated washing, the washing water yield is counted 1: 2.5 with starch weight, swirler feed pressure 0.45MPa, overflow 0.2MPa, wake flow 0.2MPa, 3 grades of light phases of concentrated higher level's eddy flow washing gained, the starch in the concentrated solution is reclaimed in 6 grades of secondary separation washings.
(5) dehydration
With the heavy phase and the recovery gained starch milk mixing of twice eddy flow washing gained, by the plate-and-frame filter press dehydration that Hangzhou Beit Filter Co., Ltd produces, remove moisture wherein, make moisture content of material be lower than 50% concentration expressed in percentage by weight;
(6) air stream drying
Pulsed gas flow drying installation by the production of control Jiangyin golden hair drying plant company limited, make its inlet temperature at 150 ℃, temperature out is below 60 ℃, the material that step (5) obtains is further dry, obtain described Starch rice 681kg, adopt the methods analyst of describing in this specification sheets to obtain: starch content 81.5%, residual protein content 0.38, yield 91.2%.
Claims (10)
1. a method that adopts the Production by Enzymes Starch rice is characterized in that described method comprises the steps:
(1) pre-treatment of cracking rice
According to the weight ratio 1 of cracking rice with water: 1-2, crack rice once with water rinse, discard washing water of rice, with the water logging bubble of the same amount 2-3h that cracks rice, pulverize then again, slurry obtains cracking rice;
(2) defibrination
Step (1) obtains, and the slurry of cracking rice grinds to form Rice ﹠ peanut milk with colloidal mill, add then entry with the concentration adjustment of Rice ﹠ peanut milk to 7-8 ° of B é, with aqueous sodium hydroxide solution its pH value is adjusted to 8.5-9.0 again;
(3) enzyme reaction
The Rice ﹠ peanut milk that step (2) is obtained is heated to temperature 50-60 ℃, add then in the quality 0.4-0.6% Sumizyme MP of cracking rice, stir, carry out enzyme digestion reaction 1.5-2.5h, add again in the quality 0.4-0.6% neutral protease of cracking rice, continue enzyme digestion reaction 2.5-3.5h, thereby obtain a kind of enzymolysis solution;
(4) eddy flow washing
The enzymolysis solution that step (3) is obtained is delivered to cyclone separator and is carried out separating, washing and concentrate, and reclaims eddy flow washing heavy phase and starch milk;
(5) dehydration
With described eddy flow washing heavy phase and described starch milk mixing, by the filter press dehydration, make the moisture weight percentage in its material be lower than 50% then;
(6) air stream drying
The material that step (5) obtains carries out drying by the pulsed gas flow drying installation again, obtains described Starch rice.
2. method according to claim 1, the fineness of pulverizing that it is characterized in that cracking rice in the described step (1) is-60 orders.
3. method according to claim 1 is characterized in that in the described step (2), and the Rice ﹠ peanut milk after colloidal mill grinds is by 80 mesh sieves.
4. method according to claim 1 is characterized in that the concentration expressed in percentage by weight of aqueous sodium hydroxide solution described in the described step (2) is 35-45%.
5. method according to claim 1 is characterized in that in the described step (3) that the Rice ﹠ peanut milk that step (2) obtains is heated to temperature 50-55 ℃.
6. method according to claim 1, the enzyme work that it is characterized in that described Sumizyme MP is 200,000 IU/g; The enzyme work of neutral protease is 130,000 IU/g.
7. method according to claim 1 is characterized in that described cyclone separator is 18 grades of cyclone separators, and wherein 9 grades are separated washing, 3 grades of concentrated and 6 grades of secondary separations washings.
8. method according to claim 1 is characterized in that in the described step (4), and the washing water yield of eddy flow washing counts 1 with starch weight: 2-2.5, swirler feed pressure 0.45-0.6MPa, oil pressure relief 0.2-0.3MPa, wake flow pressure 0.1-0.2MPa.
9. method according to claim 1 is characterized in that in the described step (6), the inlet temperature of pulsed gas flow drying installation is 130-150 ℃, and temperature out is lower than 60 ℃.
10. the Starch rice for preparing according to the described method of each claim of claim 1-9 is characterized in that the rice starch granule granularity is 2-8 μ m, structural integrity, wherein albumen weight percentage≤0.35%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110090114A CN102180980B (en) | 2011-04-12 | 2011-04-12 | Method for producing rice starch by adopting enzyme method and product obtained by using method |
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| CN102771703A (en) * | 2012-08-14 | 2012-11-14 | 莆田市东南香米业发展有限公司 | New method for producing rice noodles |
| CN102876762A (en) * | 2012-09-25 | 2013-01-16 | 安徽省旌德县辉煌粮油有限责任公司 | Processing technology for extracting protein by broken rice enzyme method |
| CN103392987A (en) * | 2013-08-15 | 2013-11-20 | 江南大学 | Method for producing high quality glutinous rice powder and soluble glutinous rice protein through enzyme method |
| CN103609830A (en) * | 2013-11-15 | 2014-03-05 | 安徽佳力宝生物科技有限公司 | Extraction method of rice protein |
| CN104039164A (en) * | 2011-11-14 | 2014-09-10 | 龟田制菓株式会社 | Rice-protein composition and method for manufacturing same |
| CN104630309A (en) * | 2015-01-30 | 2015-05-20 | 广西轻工业科学技术研究院 | Method for extracting small granule starch from manioc waste |
| CN105348395A (en) * | 2015-12-04 | 2016-02-24 | 益阳市佑林米业有限公司 | Method for extracting starch from broken rice and extracting device |
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| CN104039164A (en) * | 2011-11-14 | 2014-09-10 | 龟田制菓株式会社 | Rice-protein composition and method for manufacturing same |
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| CN102771703A (en) * | 2012-08-14 | 2012-11-14 | 莆田市东南香米业发展有限公司 | New method for producing rice noodles |
| CN102876762A (en) * | 2012-09-25 | 2013-01-16 | 安徽省旌德县辉煌粮油有限责任公司 | Processing technology for extracting protein by broken rice enzyme method |
| CN103392987A (en) * | 2013-08-15 | 2013-11-20 | 江南大学 | Method for producing high quality glutinous rice powder and soluble glutinous rice protein through enzyme method |
| CN103609830B (en) * | 2013-11-15 | 2016-05-18 | 安徽佳力宝生物科技有限公司 | A kind of rice protein extracting method |
| CN103609830A (en) * | 2013-11-15 | 2014-03-05 | 安徽佳力宝生物科技有限公司 | Extraction method of rice protein |
| CN104630309A (en) * | 2015-01-30 | 2015-05-20 | 广西轻工业科学技术研究院 | Method for extracting small granule starch from manioc waste |
| CN104630309B (en) * | 2015-01-30 | 2018-01-09 | 广西轻工业科学技术研究院 | The method that starch granule is extracted from manioc waste |
| CN105348395A (en) * | 2015-12-04 | 2016-02-24 | 益阳市佑林米业有限公司 | Method for extracting starch from broken rice and extracting device |
| CN107226868A (en) * | 2017-07-21 | 2017-10-03 | 江南大学 | A kind of middle method for testing for producing high-purity chestnut starch |
| CN108314743A (en) * | 2018-03-22 | 2018-07-24 | 山东省科学院生物研究所 | A kind of Wetting technique for soaking corn |
| CN108314743B (en) * | 2018-03-22 | 2020-11-24 | 山东省科学院生物研究所 | Novel wet corn soaking process |
| CN112641041A (en) * | 2020-09-25 | 2021-04-13 | 湖南康洁食品科技发展有限公司 | Method for producing tasty instant rice noodles by using broken rice |
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