CN115417936A

CN115417936A - Method for extracting pectin from waste beet pulp after sugar production by double-enzyme method

Info

Publication number: CN115417936A
Application number: CN202211298443.4A
Authority: CN
Inventors: 孟赫诚; 周景园; 李丽丽; 谢莞霖; 吕智浩
Original assignee: South China University of Technology SCUT; Jinan University
Current assignee: South China University of Technology SCUT; Jinan University
Priority date: 2022-10-23
Filing date: 2022-10-23
Publication date: 2022-12-02
Anticipated expiration: 2042-10-23
Also published as: CN115417936B

Abstract

The invention belongs to the technical field of plant component extraction, and discloses a method for extracting pectin from waste beet pulp after sugar production by a two-enzyme method. The method comprises the following steps: cleaning, drying and crushing beet pulp, then carrying out high-temperature cooking extraction, adding the mixture into a cellulose complex enzyme solution, carrying out enzymatic extraction to obtain a beet pectin solution, adding 95% ethanol for precipitation, and washing the obtained precipitate with an ethanol-water solution and drying the precipitate in an oven to obtain the beet pectin. Compared with the prior acid extraction method, oxalic acid-ammonium oxalate extraction method, fermentation method and the like, the pectin extraction rate is improved from 10-12% to 13-19%, and the emulsifying activity and the emulsifying stability of the beet pectin are improved.

Description

Method for extracting pectin from waste beet pulp after sugar production by double-enzyme method

Technical Field

The invention relates to the technical field of plant component extraction, in particular to a method for extracting pectin from waste beet pulp after sugar refining by a double-enzyme method.

Background

Pectin has excellent gelling, thickening, emulsifying, stabilizing and other functional properties, and is recommended by the food additive association committee as a safe natural food additive without limiting the daily allowable intake. According to the degree of esterification, pectins can be classified into high-ester pectins and low-ester pectins. Most natural pectins are high-ester pectins and few low-ester pectins. Because the pectin can be gelled under the condition of low sugar or no sugar, the low-ester pectin is widely used for producing low-sugar jam, jelly, yoghourt or sugar-free health food and the like. However, due to the limitation of production technology, the yield of low-ester pectin in China is far less than the demand amount, and the low-ester pectin still depends on foreign import so far, thereby seriously restricting the development of related food industries in China.

Pectin is one of the main active ingredients in beet pulp. The beet pulp is used as the main byproduct of beet sugar production, and the pectin content reaches 25 percent. The process of converting low cost into high value-added products is realized by extracting pectin from beet pulp. Beet pectin can be used for preparing milk beverages due to its very good emulsifying property, and can be widely used as a food additive in the baking industry and the jam manufacturing industry due to its various properties. Pectin is widely present in the cell wall of plants, and is one of the supporting substances and plant cell wall components inside plant cells. The principle of extracting pectin from plant cell walls is to break the cell wall structure of the plant in the presence of an extractant or a certain physical action to separate pectin bound to other polysaccharides in the cell wall.

The pectin extracting method mainly comprises the traditional acid-heat extraction method, ultrasonic-assisted extraction method, enzyme extraction method, chelating agent extraction method and the like. However, the traditional acid-heat extraction method has larger damage degree to pectin and is easy to cause pollution, and compared with the traditional acid extraction method, the enzyme method has mild conditions and no pollution.

In addition, we have found that drying takes a lot of time during the experiment, and the material needs to be transferred after drying is completed, so that time is wasted and the possibility of material pollution is possibly existed.

Disclosure of Invention

The purpose of the invention is as follows: in order to provide a better two-enzyme method for extracting pectin from beet pulp discarded after sugar refining, a specific purpose is to see a plurality of substantial technical effects of a specific implementation part.

In order to achieve the purpose, the invention adopts the following technical scheme:

the method for extracting pectin from the waste beet pulp after sugar production by the double-enzyme method is characterized by comprising the following steps:

(1) Cleaning sugar beet pulp, removing impurities, drying, and pulverizing;

(2) Adding the crushed beet pulp powder into a buffer solution with the pH value of 4.0-5.0, wherein the buffer solution is a citric acid-sodium citrate buffer solution, then integrally placing the buffer solution into an autoclave for high-temperature cooking, and taking out the buffer solution after the pressure and temperature reduction are finished to room temperature; the high-temperature cooking condition is that the solid-to-liquid ratio of the beet pulp is 1:20g/mL, the temperature is 121 ℃, and the extraction time is 15min; the pressure is 50kpa;

(3) Taking out the mixture obtained in the step (2), adjusting the pH value to be 4.0-5.0, adding a mixed solution of cellulose and hemicellulase, stirring and extracting, heating the solution to inactivate enzyme, filtering and separating to realize first impurity removal, wherein undersize materials obtained by filtering and separating are solid residues, and thus a beet pulp pectin solution and the solid residues obtained by enzymatic extraction are obtained; the enzyme adding amount is 40-100 mu L, and the ratio of the cellulase to the hemicellulase is 2:1, the extraction temperature is 45-55 ℃, the extraction mode is that the extraction is carried out in a water bath kettle under stirring, and the extraction time is 1-2h;

(4) Centrifuging the pectin solution obtained in the step (3) to remove protein impurities, realizing secondary impurity removal, taking a centrifuged supernatant, adjusting the pH to 2.0-3.0, adding 95% ethanol while stirring, standing until pectin is separated out, filtering with a filter cloth, and washing with an ethanol-water solution to obtain beet pectin; the centrifugation conditions in the step (4) are 4 ℃,8000rpm and 20min; adjusting pH with citric acid solution, adding 95% ethanol 3 times of supernatant volume; the ethanol-water solution washing refers to washing with 75% and 95% ethanol respectively, and the ethanol washing is to remove alcohol-soluble impurities, which is the third impurity removal.

The further technical scheme of the invention is that the drying is blast drying until the moisture content is below 8%.

The invention further adopts the technical scheme that the filter cloth used in the filtering in the step (3) is 400-mesh filter cloth.

The further technical scheme of the invention is that the drying, the crushing and the adding of the subsequent citric acid-sodium citrate buffer solution are completed by a special machine for batch production;

the special machinery of batch production is dedicated dry crushing compounding all-in-one, dry crushing compounding all-in-one structure as follows:

a circumferential ventilation structure and an internal multipurpose drying and cutting structure which are arranged concentrically are arranged;

the circumferential ventilation structure is a cylindrical structure with a circular section, a cavity 11 in the cylindrical structure is connected with a fan vent 6, and a circle of air dispersing openings 7 are arranged on the inner wall of the cylindrical structure;

the multipurpose drying and cutting structure comprises a perforated cylinder 1, an opening is arranged above the perforated cylinder 1, the body of the perforated cylinder 1 is cylindrical, an arc-shaped bottom wall is arranged below the body, and a plurality of powder through holes are formed in the body and the bottom wall of the perforated cylinder 1; still contain motor shaft 3 that can rotate at the middle part of taking a hole section of thick bamboo 1, arranged multiunit cutting stirring piece 4 on motor shaft 3, motor shaft 3 is connecting motor 2.

The further technical proposal of the invention is that the plurality of groups of cutting and stirring blades 4 are blades with blades.

The invention further adopts the technical scheme that a material powder storage area 8 is arranged below the circumferential ventilation structure, the side of the material powder storage area 8 is connected with a buffer solution injection port 9, and a mixture outlet pipe 10 is also arranged below the material powder storage area 8.

The further technical scheme of the invention is that a weight sensor is arranged in the material powder storage area 8, and a flow meter is arranged on a pipeline of the buffer solution injection port 9.

A further technical solution of the present invention is that the mixture outlet pipe 10 is connected to an autoclave.

The invention further adopts the technical scheme that beet pulp is put into a perforated cylinder, then air is blown through an air vent of a fan, the air is diffused from a cavity through an air diffuser and blown to the material in the perforated cylinder, then the material is dried, in the drying process, a motor drives a cutting stirring sheet to be properly stirred, after the material is dried, the power of the motor is increased, the cutting stirring sheet is enabled to rotate at a high speed, the material is crushed to a proper particle size and falls down through a powder through hole of the perforated cylinder, the material enters a material powder storage area, then a buffer solution is put into the material powder storage area through a buffer solution injection port, and a mixture outlet pipe enters the next process.

Compared with the prior art, the invention adopting the technical scheme has the following beneficial effects: (1) The invention has high pectin extraction rate, low esterification degree, small relative molecular weight and light color; the content of galactaric acid of the pectin is high;

(2) The invention firstly proposes a two-step method for extracting beet pulp pectin, namely a high-temperature cooking and an enzyme method, compared with the acid-heating method, a chelating agent method and the like in the prior art, the extraction rate of the pectin is improved from 10-12% to 13-17%, and the emulsifying activity and the emulsifying stability are improved;

(3) Compared with the prior art, the extraction method has the advantages of no pollution and low pectin damage degree.

(4) The large-scale production and the drying and crushing are integrated; no complicated material is used for transferring. Can realize continuous industrial production, basically does not need a belt conveyor to transport materials after drying, and can realize the configuration of automatic buffer solution and powder.

Drawings

To further illustrate the present invention, further description is provided below with reference to the accompanying drawings:

FIG. 1 is a GPC molecular weight measurement chart of beet pulp pectin obtained in example 1 of the present invention;

FIG. 2 is an infrared scanning spectrum of beet pulp pectin obtained in example 1 of the present invention;

FIG. 3 is a plot of the galactonic acid signature of beet pulp pectin obtained in example 1 of the present invention;

FIG. 4 is a graph showing the distribution of particle sizes of emulsions prepared from beet pulp obtained in the application example of the present invention at normal temperature for 0, 1, 3, 7 and 14 days; with the prolonging of the storage time, the emulsion has slight instability, the single-peak direction of the particle size distribution slightly deviates, but the distribution has small change on the whole, the phenomenon of serious fracture and instability does not occur, and the stability is better;

FIG. 5 is an optical microscope photograph of an emulsion prepared from beet pulp obtained in the example of the application of the present invention;

FIG. 6 is an appearance diagram of an emulsion prepared from beet pulp obtained in the application example of the present invention;

FIG. 7 is a schematic illustration of a combination machine of steps one and two;

FIG. 8 is a schematic cross-sectional view of FIG. 7;

FIG. 9 is a schematic view from another perspective of FIG. 7;

wherein: 1. a perforated cylinder; 2. a motor; 3. a motor shaft; 4. cutting the stirring sheet; 5. a cavity; 6. a fan vent; 7. an air diffuser; 8. a material powder storage area; 9. a buffer solution injection port; 10. a mixture outlet pipe; 11. a cavity.

Detailed Description

The present invention will be further illustrated with reference to the accompanying drawings and detailed description, which will be understood as being illustrative only and not limiting in scope. In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", "top", "bottom", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention. Furthermore, unless expressly stated or limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, as they may be fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

The patent provides a plurality of parallel schemes, and different expressions belong to an improved scheme based on a basic scheme or a parallel scheme. Each solution has its own unique features. In addition, the technical features involved in the different embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other. The fixing means, which is not described herein, may be any one of screw fixing, bolt fixing, or glue bonding.

Example 1

Cleaning sugar beet pulp with clear water for several times, removing impurities, air drying at 50 deg.C for 12 hr, mechanically pulverizing dried beet pulp with pulverizer for 30min to obtain beet pulp fine powder, sieving with 60 mesh sieve, sealing in sample bag, and storing at room temperature; adding the crushed and sieved beet pulp powder into the mixture according to the solid-liquid ratio of 1:20 Adding 600mL of citric acid-sodium citrate buffer solution (pH 4.5) according to the proportion of (W/V, g/mL), uniformly stirring, wrapping with gauze, wrapping with newspaper, sealing with cotton rope, placing into an autoclave, setting at 121 ℃ for 15min, reducing the pressure, cooling to 100 ℃, taking out an ice water bath, and cooling. After cooling, adjusting the pH value to 4.5 by using citric acid, adding 0.6g of cellulase and 0.3g of hemicellulase, uniformly stirring, carrying out water bath at 50 ℃ and 270rpm for 2h, heating the extract to 100 ℃, continuously boiling for 5min for enzyme deactivation, quickly taking out an ice water bath, cooling to room temperature, filtering by using 400-mesh filter cloth, centrifuging the pectin crude extract at 8000rpm/min, 20min and 4 ℃, adjusting the pH value of a supernatant to 3.0 by using citric acid, adding 95% ethanol solution with three times of volume while stirring, standing at room temperature for 1h, filtering by using 400-mesh filter cloth, washing by using 75% and 95% ethanol aqueous solution for 2 times in sequence, and drying to constant weight in a drying box at 40 ℃ to obtain the beet pulp pectin. Tests show that the beet pulp pectin yield is 14% (W/W, g/g), the uronic acid content is 68%, and the molecular weight is 312586Da.

Example 2

Cleaning sugar beet pulp with clear water for several times, removing impurities, air drying at 50 deg.C for 12 hr, mechanically pulverizing dried beet pulp with pulverizer for 30min to obtain beet pulp fine powder, sieving with 60 mesh sieve, sealing in sample bag, and storing at room temperature; adding the crushed and sieved beet pulp powder into the mixture according to the solid-to-liquid ratio of 1:20 Adding 600mL of citric acid-sodium citrate buffer solution (pH 4.5) according to the proportion of (W/V, g/mL), uniformly stirring, wrapping with gauze, wrapping with newspaper, sealing with cotton rope, placing into an autoclave, setting at 121 ℃ for 15min, reducing the pressure, cooling to 100 ℃, taking out an ice water bath, and cooling. After cooling, adjusting the pH value to 4.5 by using citric acid, adding 0.6g of cellulase and 0.3g of hemicellulase, uniformly stirring, carrying out water bath at 50 ℃ and 270rpm for 1h, heating the extract to 100 ℃, continuously boiling for 5min for inactivating enzyme, quickly taking out the extract, cooling to room temperature, filtering by using 400-mesh filter cloth, centrifuging the pectin crude extract at 8000rpm/min, 20min and 4 ℃, adjusting the pH value of the supernatant to 3.0 by using citric acid, adding three times of 95% ethanol solution in volume while stirring, standing at room temperature for 1h, filtering by using 400-mesh filter cloth, washing by using 75% and 95% ethanol aqueous solution for 2 times in sequence, and drying in a drying box at 40 ℃ to constant weight to obtain the beet pulp pectin. Tests show that the beet pulp pectin yield is 13.22% (W/W, g/g), the uronic acid content is 67%, and the molecular weight is 309256Da.

Example 3

Cleaning sugar-processed beet pulp with clear water for several times, removing impurities, air drying at 50 deg.C for 12 hr, mechanically pulverizing dried beet pulp with a pulverizer for 30min to obtain beet pulp fine powder, sieving with 60 mesh sieve, sealing in sample bag, and storing at room temperature; adding the crushed and sieved beet pulp powder into the mixture according to the solid-to-liquid ratio of 1:20 Adding 600mL of citric acid-sodium citrate buffer solution (pH 4.5) according to the proportion of (W/V, g/mL), uniformly stirring, wrapping with gauze, wrapping with newspaper, sealing with cotton rope, placing into an autoclave, setting at 121 ℃ for 15min, reducing pressure, cooling to 100 ℃, taking out, and cooling in ice water bath. After cooling, adjusting the pH value to 4.5 by using citric acid, adding 0.6g of cellulase and 0.6g of hemicellulase, uniformly stirring, carrying out water bath at 50 ℃ and 270rpm for 1h, heating the extract to 100 ℃, continuously boiling for 5min for inactivating enzyme, quickly taking out the extract, cooling to room temperature, filtering by using 400-mesh filter cloth, centrifuging the pectin crude extract at 8000rpm/min, 20min and 4 ℃, adjusting the pH value of the supernatant to 3.0 by using citric acid, adding three times of 95% ethanol solution in volume while stirring, standing at room temperature for 1h, filtering by using 400-mesh filter cloth, washing by using 75% and 95% ethanol aqueous solution for 2 times in sequence, and drying in a drying box at 40 ℃ to constant weight to obtain the beet pulp pectin. The test shows that the beet pulp pectin yield is 13% (W/W, g/g), the uronic acid content is 68%, and the molecular weight is 326025Da.

0.5g of pectin obtained in inventive example 1 was dissolved in 25ml of citric acid buffer (pH 3.5, concentration 50Mm, sodium benzoate content 0.1%), stirred overnight to dissolve it sufficiently, 7.5g of medium-chain fatty acid was added, and citric acid buffer was added to 50g. Pre-homogenizing with a handheld high-speed shearing machine at 20000rpm for 2 min, and further homogenizing with an ultrasonic cell disruptor at 3min,2s/2s. Storing the emulsion at normal temperature, and measuring the particle size of the emulsion on 0 th, 1 th, 3 th, 5 th, 7 th and 14 th days, and taking an optical microscope picture and an appearance picture.

The emulsion in the application example has slight deviation of a single peak of the emulsion particle size distribution to a large particle size direction, a small amount of large-particle-size droplets appear after the storage time exceeds 3 days, the proportion of the droplets slowly increases along with the time, and although the changes occur in the storage process, the emulsion particle size distribution has small change on the whole, no serious fracture instability phenomenon occurs, and the stability is better.

It should be noted that the plurality of schemes provided in this patent include their own basic schemes, which are independent of each other and are not restricted to each other, but they may be combined with each other without conflict, so as to achieve a plurality of effects.

Example four: as a further improvement or a parallel scheme or an alternative independent scheme, the drying, the crushing and the adding of the subsequent citric acid-sodium citrate buffer solution are completed by a special machine in batch production;

batch production's purpose-built machinery is dedicated dry crushing compounding all-in-one, dry crushing compounding all-in-one structure as follows:

the circumferential ventilation structure is a cylindrical structure with a circular section, a cavity 11 in the cylindrical structure is connected with a fan ventilation opening 6, and a circle of air dispersing openings 7 are arranged on the inner wall of the cylindrical structure;

the multipurpose drying and cutting structure comprises a perforated cylinder 1, an opening is arranged above the perforated cylinder 1, the body of the perforated cylinder 1 is cylindrical, an arc-shaped bottom wall is arranged below the body, and a plurality of powder through holes are formed in the body and the bottom wall of the perforated cylinder 1; still contain motor shaft 3 that can rotate at the middle part of taking a hole section of thick bamboo 1, arranged multiunit cutting stirring piece 4 on motor shaft 3, motor shaft 3 is connecting motor 2. The technical scheme of the invention has the following substantial technical effects and the realization process thereof: beet pulp is put into a perforated cylinder, then the fan vent blows, wind distributes through the scattered wind gap in the cavity and blows to the material in the perforated cylinder after the perforated cylinder, carry out the drying, dry in-process, the motor drives the cutting stirring piece and suitably stirs, after the drying, increase motor power afterwards, let the cutting stirring piece rotate at a high speed, the material is smashed and is fallen down in the powder clearing hole through the perforated cylinder after suitable particle diameter, get into the material powder and deposit the region, put into buffer solution through the buffer solution filling opening afterwards, the mixture outlet pipe gets into next process.

The large-scale production and the drying and crushing are integrated; no complicated material is used for transferring. Can realize continuous industrial production, basically does not need a belt conveyor to transport materials after drying, and can realize the configuration of automatic buffer solution and powder. Is an important step of the laboratory to the industrial production.

Example five: as a further improvement scheme or a side-by-side scheme or an alternative independent scheme, the multiple groups of cutting and stirring blades 4 are blades with blades. The technical scheme of the invention has the following substantial technical effects and the realization process: can conveniently cut.

Example six: as a further improvement or a parallel scheme or an alternative independent scheme, a material powder storage area 8 is arranged below the circumferential ventilation structure, the side of the material powder storage area 8 is connected with a buffer solution injection port 9, and a mixture outlet pipe 10 is further arranged below the material powder storage area 8.

Example seven: as a further modification or a side-by-side or alternatively an independent solution, a weight sensor is disposed in the material powder storage area 8, and a flow meter is disposed on a pipe of the buffer solution injection port 9. The mixture outlet pipe 10 is connected to an autoclave.

The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are merely illustrative of the principles of the invention, but various changes and modifications may be made without departing from the spirit and scope of the invention, which is defined by the appended claims.

Claims

1. The method for extracting pectin from the waste beet pulp after sugar production by the double-enzyme method is characterized by comprising the following steps:

(1) Cleaning beet pulp after sugar production, removing impurities, drying, and pulverizing;

(4) Centrifuging the pectin solution obtained in the step (3) to remove protein impurities, realizing secondary impurity removal, taking a centrifuged supernatant, adjusting the pH to 2.0-3.0, adding 95% ethanol while stirring, standing until pectin is separated out, filtering with a filter cloth, and washing with an ethanol-water solution to obtain beet pectin; the centrifugation conditions in the step (4) are 4 ℃,8000rpm and 20min; adjusting pH with citric acid solution, and adding 95% ethanol with 3 times of supernatant volume; the ethanol-water solution washing refers to washing with 75% and 95% ethanol respectively, and the ethanol washing is to remove alcohol-soluble impurities, which is the third impurity removal.

2. The method for extracting pectin from beet pulp discarded after sugar manufacturing by the two-enzyme method according to claim 1, wherein the drying is air drying until the moisture content is below 8%.

3. The method for extracting pectin from beet pulp discarded after sugar manufacture by the two-enzyme method according to claim 1, wherein the filter cloth used in the filtering of step (3) is 400 mesh filter cloth.

4. The method for extracting pectin from beet pulp discarded after sugar manufacturing by the two-enzyme method according to claim 1, wherein the drying, pulverizing and adding the subsequent citric acid-sodium citrate buffer solution are performed by a special machine for mass production;

the circumferential ventilation structure is a cylindrical structure with a circular section, a cavity (11) in the cylindrical structure is connected with a fan ventilation opening (6), and a circle of air dispersing openings (7) are arranged on the inner wall of the cylindrical structure;

the multipurpose drying and cutting structure comprises a perforated cylinder (1), an opening is arranged above the perforated cylinder (1), the body of the perforated cylinder (1) is cylindrical, an arc-shaped bottom wall is arranged below the body of the perforated cylinder (1), and a plurality of powder through holes are formed in the body and the bottom wall of the perforated cylinder (1); still contain middle part pivoted motor shaft (3) that can take a hole section of thick bamboo (1), arranged multiunit cutting stirring piece (4) on motor shaft (3), motor (2) are being connected in motor shaft (3).

5. The method for extracting pectin from sugar beet pulp discarded after sugar production by the two-enzyme method according to claim 4, wherein the plurality of cutting stirring blades (4) are blades with blades.

6. The method for extracting pectin from sugar beet pulp discarded after sugar production by the two-enzyme method according to claim 4, wherein a material powder storage area (8) is arranged below the circumferential ventilation structure, the side of the material powder storage area (8) is connected with a buffer solution inlet (9), and a mixture outlet pipe (10) is arranged below the material powder storage area (8).

7. The method for extracting pectin from sugar beet pulp discarded after sugar production by the two-enzyme method according to claim 6, wherein a weight sensor is disposed in the material powder storage area (8), and a flow meter is disposed on a pipe of the buffer solution inlet (9).

8. The method for extracting pectin from sugar beet pulp discarded after sugar production by the two-enzyme method according to claim 6, wherein the mixture outlet pipe (10) is connected to an autoclave.

9. The method for extracting pectin from beet pulp discarded after sugar manufacture by the two-enzyme method according to claim 6, wherein the beet pulp is put into a perforated cylinder, then a ventilation opening of a fan blows, the air is diffused from the cavity through an air diffusing opening and blown to the material in the perforated cylinder for drying, during the drying process, a motor drives a cutting and stirring piece to properly stir, after the drying process, the power of the motor is increased, the cutting and stirring piece is rotated at high speed, the material is crushed to a proper particle size and falls down through a powder through hole of the perforated cylinder to enter a material powder storage area, then a buffer solution is put through a buffer solution inlet, and a mixture outlet pipe enters the next process.