CN103232555B - A kind of method utilizing enzyme process to extract pectin from lemon peel slag - Google Patents
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Abstract
A kind of method utilizing enzyme process to extract pectin from lemon peel slag disclosed by the invention first lemon peel slag is carried out pre-treatment, then be the damping fluid mixing of 4 ~ 8 by weight 1:15 ~ 1:30 and pH, add again with lemon peel slag weighing scale 0.01 ~ 0.3% zymin stir and at 40 ~ 70 DEG C, be incubated extraction 1 ~ 5h, continue after centrifugation successively, vacuum concentration, cooling, decolouring, alcohol precipitation, desugar, drying and pulverizing after namely obtain pectin powder finished product.Because the present invention is while use zymin, also added pH be 4 ~ 8 damping fluid mixed to coordinate with it, thus the extraction time of pectin is not only substantially reduced, improve extraction efficiency, decrease the usage quantity of zymin, reduce extraction cost, and also improve pectin extraction rate and product purity, in addition proteolytic enzyme used is mostly the natural plant extracted in plant, and price is lower, is more conducive to realizing its commercial applications.
Description
Technical field
The invention belongs to and utilize Enzymatic Extraction pectin technical field, be specifically related to a kind of method utilizing enzyme process to extract pectin from lemon peel slag.
Background technology
Along with modern nectar industrial expansion, from fresh fruit squeezing juice the course of processing in a large amount of discarded pomaces of producing, fail for a long time to be utilized effectively, cause the huge waste of resource, simultaneously also serious environment pollution.If carry out comprehensive process to these processing fruits wastes, resource not only can be made to be used adequately reasonably, greatly to improve the overall economic efficiency of processing fruits, also can reduce the pollution to environment.Thus, a lot of scientific worker begins one's study and extract soluble dietary fibre from discarded pomace---pectin.
Pectin is identified as one of 21st century food function composition, is called as " balancing agent of HUMAN HEALTH ".Pectin is mainly used in foodstuff additive, as thickening material, gelifying agent, stablizer, for fat agent, emulsifying agent etc., also can be used as water-soluble dietary fibre for reducing blood pressure and blood lipoid, promote the absorption of VITAMIN, prevent lead poisoning, prevent and treat the health cares such as arteriosclerosis (Voragen AGJ, et al. Struct Chem, 2009,20,263).
Current report pectin starting material is more than 40 kinds, but the main raw material of commodity pectin is still peel of Citrus reticulata Blanco and Pericarpium Mali pumilae (May CD. Carbohydrate Polymers, 1990,12 (1): 79).Lemon is a kind of excellent body-care fruit, there is very high economic worth, the lemon of China processes the products such as mainly production lemon oil, lemon juice (lemon beverage), lemon dryslice, and based on roughing, so lemon about produces the skin slag of 40 ~ 50% after roughing.After deliberation, lemon peel slag is rich in pectin, and higher than general Citrus raw material pectin content, and orange peel residue pectin content is only 20%, and the pectin content of lemon peel slag can up to 30%(Hai Xiang Wei etc. food research and development, 2006,27,157).Moreover, in citrus pectin or all pectin, performance is best, and color and luster is best, the pectin that price is also the highest, but does not regrettably domesticly at present also have commercial citrus pectin.
The main method of existing extraction pectin have classical acid extraction method, ion exchange method, microwave method, microbial method, Continuous Countercurrent Extraction method, enzyme process etc. (Tian Hui etc. Food Additives Used in China, 2007, (1): 126).
Domestic goods pectin production many employings traditional method---acid extraction ethanol precipitation.Even so, but the shortcoming that sour formulation exists is obvious: 1) pectin molecule, in leaching process, local hydrolysis easily occurs, and can reduce the relative molecular weight of pectin, affect pectin yield and quality; 2) need use a large amount of mineral acids when extracting, this can increase cost on the one hand, on the other hand acid solution meeting etching apparatus, and has pollution to environment; 3) when extracting pectin with alcohol settling, the amount of alcohol of consumption is too large, in addition high the and recycling difficulty of ethanol price, and make the cost of extraction too high, economic benefit is not obvious.So people are devoted to improve acid technological process.As (foodstuffs industry science and technology, 2007,28 (6): 182) such as Xu Dan improve pretreatment process, namely adopt microwave deactivating enzyme and composite phosphate pre-treatment lemon peel, thus improve pectin yield.Liu Yiwu etc. (food research and development, 2012,33 (9): 76) then adopt organic acid to extract pectin from lemon peel, significantly reduce the erosion rate of acid solution to equipment.It is raw material with lemon peel that Wu Yongxian etc. disclose a kind of in CN 1107858, the method for acid extraction pectin.The method ultrafiltration and concentration replaces vacuum concentration, overcomes the shortcoming that vacuum concentration energy consumption is high.It is raw material with lemon peel that Lv Weixue discloses a kind of in CN102731679, the method of acid extraction, combining ultrafiltration nanofiltration, spraying dry extraction pectin, reduce the usage quantity of organic solvent, remain original biological activity, just can increase effective period of food quality, nourishing function etc. when pectin adds in food like this.Masmoudi M etc. find again to extract pectin from lemon peel with acidifying jujube juice, can increase local flavor and the gellifying property of pectin from lemon peel and reduce pectin esterification degree (Carbohyd Polym, 2008,74,185; Food Sci Technol Int, 2010,16,105; Food Bioproc Technol 2012,5,687.).The research of the people such as Wan Guofu shows effectively to improve pectin from lemon peel extraction yield (foodstuffs industry science and technology by composite phosphate pre-treatment; 27 (2): 78); and utilize ultrasonic wave, microwave-assisted can improve pectin from lemon peel extraction yield (Wan Guofu etc. food research and development; 2006,27 (7): 115; Li Mingyuan. food research and development, 2007,28 (5): 91).
Ion exchange method production cost is high, needs higher temperature and long-time heating, and the pectin contained in raw material inevitably produces sex change Sum decomposition and destroys, and reduces the quality and quantity of pectin extraction.Microwave rule exists: microwave heating speed is too fast, wayward Heating temperature; The wavelength of microwave and power need strict control, otherwise can cause pectin product form difference to some extent; Microwave method misoperation is easily leaked, and produces harm to biology.Microbial method extract pectin by raw material pre-treatment, reaction time solid-to-liquid ratio, the microbial growth time, soaking time and pH value impact larger.So these extracting method are all difficult to realize industrialization.
Enzymatic Extraction pectin has phase of history.In the phase at the end of the twenties in last century, Paul etc. just take off ester with Rohapect MPE and prepare low-ester pectin.Sakamoto etc. adopt protopectinase to extract pectin (Process Biochemistry, 1995,30 (5): 403).(the Food Hydrocolloids such as Shkodina, 1998,12 (3): 313) then by comparing acid system and enzyme process (cellulase, hemicellulase and Glycosylase) extracts pectin from pumpkin, find that the output of Enzymatic Extraction pectin is all higher than acid system, the output of its cellulase is the highest, be acid system twice more than, but the time reach 20 h.Zhang Yiqing etc. have studied and extract pectin in Cirtus peel with homemade protopectinase crude enzyme liquid (not having enzyme analysis content), and pectin productive rate reaches 35.7%(Food science, and 2005,26 (1): 150).The pectin such as cellulase extraction apple peel, orange peel, pineapple peel, orange peel and premna microphylla that utilize of nearest disclosure are studied also more.As Gu Zeke etc. discloses a kind of method utilizing cellulase, hemicellulase and mixed preparation thereof to extract pectin in citrus fruits, tropical fruit, apple, beet, Sunflower Receptacle, soybean and composition thereof in CN 101160327.But extraction time is longer, the longest extraction time reaches 72h.Zhao Yue etc. disclose the method adopting cellulase, hemicellulase, Glycosylase and mixed enzyme thereof by enzymolysis, ultrafiltration and concentration, drying process with atomizing continuous production high purity pectin in CN 102161713.Contreras-Esquivel etc. (Food Sci Biotechnol 2006,15,163) find that endo-polygalacturonase (genus polygalacturonase) is conducive to reducing pectin from lemon peel gamma value.Although Enzymatic Extraction pectin is because of the high efficiency of enzyme effect and specificity, pectin yield is high, the technological process of production and equipment simple, technique is easy to control, and the degree of gelation of product can be kept better, but because the enzyme used is mainly derived from microorganism culturing, therefore can because of the difference of bacterial classification, the difference of culture condition, make the composition of enzyme different, in addition the complex structure of enzyme itself, even if make to use the extraction efficiency of different manufacturers enzyme of the same race also different, and both at home and abroad in the research of Enzymatic Extraction pectin, all the cost of ubiquity extraction enzyme is high, be difficult to realize industrialization problem (Han Dongmei etc. foodstuffs industry science and technology, 2011, 32 (2): 183).
Summary of the invention
The object of the invention is from lemon peel slag, to extract pectin Problems existing for existing enzyme process, a kind of method utilizing enzyme process to extract pectin from lemon peel slag is provided.
A kind of method utilizing enzyme process to extract pectin from lemon peel slag provided by the invention, processing step and the condition of the method are as follows:
1) lemon peel slag is put in the water of tare weight 2 ~ 3 times, heated and boiled 5 ~ 10min, with clear water rinsing to colourless, dry, pulverize;
2) by the damping fluid mixing that the lemon peel slag pulverized is 4 ~ 8 by weight 1:15 ~ 1:30 and pH, then add and slowly stir with lemon peel slag weighing scale 0.01 ~ 0.3% zymin, at 40 ~ 70 DEG C, be incubated extraction 1 ~ 5 h;
3) by pectin extracting soln centrifugation, gained pectin stoste is concentrated into 1/4 ~ 1/2 of concentrated front extracting liquid volume, namely cools fast;
4) in fructose concentrating liquid, add the hydrogen peroxide of fructose concentrating liquid quality meter 0.5 ~ 3%, decolour under room temperature 30 ~ 60 min, then adding 1 ~ 3 times of concentration is the ethanol of 95%, leaves standstill 30 ~ 60 min, filters, 75% washing with alcohol of 2 ~ 3 times of weight is added again in filter cake, repeatedly wash 3 ~ 5 times, filter, washing with alcohol is dewatered, pectin powder finished product is after dry, pulverize.
Aforesaid method damping fluid used is that citric acid one citric acid is received or citric acid one Sodium phosphate dibasic composition, and its ratio is 4 ~ 8 to be as the criterion with the pH regulator of damping fluid.
Aforesaid method zymin used is at least one in cellulase, hemicellulase, papoid, bromeline and ficin, the natural plant enzyme preferably extracted from plant---at least one in papoid, bromeline and ficin.
The lemon peel slag pulverized in aforesaid method is the damping fluid mixing of 6 ~ 8 by weight preferred 1:20 ~ 1:30 and pH.
The consumption of used enzyme preparation preferably 0.1 ~ 0.3% in aforesaid method; Holding temperature preferably 50 ~ 60 DEG C, soaking time is 2 ~ 3 h preferably; Hydrogen peroxide consumption preferably 2.0%, bleaching time is 50 ~ 60 min preferably.
Hydrogen peroxide used in aforesaid method is commercially available concentration is the hydrogen peroxide of 30%.
Compared with prior art, advantage of the present invention is:
1. because the inventive method is while use zymin extracts pectin, also added pH be 4 ~ 8 damping fluid coordinate with it, thus not only substantially reduce the extraction time of pectin, improve extraction efficiency, and also reduce the usage quantity of zymin, reduce extraction cost.
2. because the inventive method adopts natural plant enzyme to extract pectin, under the prerequisite keeping higher extraction yield, not only the consumption of enzyme can reduce, and price is lower, thus both production cost can be reduced further, and product purity high (>=65%) can be improved, make it foodstuff additive---the standard of pectin meeting China (GB 25533-2010) and European Union's formulation, directly come into the market as products in circulation.
3. due to the inventive method adopt natural plant enzyme not only cost is low, consumption is few, and its pectin extraction rate is high, and the economic benefit of thus industrialization is very considerable.
4., owing to have employed the medical disinfecting level ethanol of concentration 75% in the desugar technique of the inventive method, thus reduce the impact on product such as microorganism, bacterium, further increase pectin quality.
5. operation is simple for extracting method provided by the invention, is suitable for suitability for industrialized production.
Embodiment
Below by embodiment, the present invention is specifically described; what be necessary to herein means out is that the present embodiment is only used to further illustrate the present invention; can not be interpreted as limiting the scope of the invention, the person skilled in the art in this field can make some nonessential improvement and adjustment according to the content of the invention described above.
What deserves to be explained is: extraction yield, the weight loss on drying, always galacturonic acid and gamma value of following embodiment gained pectin all measure according to the method for pectin analysis part in GB 25533-2010.
Embodiment 1
Put into by lemon peel slag in the water of tare weight 2 times, heated and boiled 5 min, to destroy the polygalacturonase in lemon peel slag, with clear water rinsing to colourless, tentatively removes the various non-colloidalmaterials such as aromatoising substance, sugar, bitter substance and pigment, dries, pulverizes; The lemon peel slag of pulverizing is received damping fluid by weight citric acid one citric acid that 1:15 and pH is 5 mix, then add and slowly stir with lemon peel slag weighing scale 0.01% cellulase, at 50 DEG C, be incubated extraction 3 h; Pectin extracting soln centrifugation is obtained pectin stoste; By the equal-volume water washing of pectin stoste, then centrifugation, merging filtrate, the filtrate after merging is concentrated into 1/2 of concentrated front extracting liquid volume, namely cools fast; In fructose concentrating liquid, add the hydrogen peroxide of fructose concentrating liquid quality meter 0.5%, decolour under room temperature 30 min, and then adding 3 times of concentration is the ethanol of 95%, leaves standstill 30min, filters; In filter cake, add 75% washing with alcohol of 2 times of weight again, repeatedly wash 3 times, filter, the washing with alcohol dehydration of 95%, is pectin powder finished product after dry, pulverize.Collect filtrate, vacuum concentration, reclaim ethanol and recycle.
The extraction yield of the present embodiment pectin is 21.2%, weight loss on drying 7.0%, total galacturonic acid 70.7%, gamma value 71.1%.
Embodiment 2
Lemon peel slag put in the water of tare weight 2 times, heated and boiled 5 min, dry, pulverize; The lemon peel slag of pulverizing is received damping fluid by weight citric acid one citric acid that 1:30 and pH is 6 mix, then add and slowly stir with lemon peel slag weighing scale 0.05% cellulase, at 40 DEG C, be incubated extraction 4 h; Pectin extracting soln centrifugation is obtained pectin stoste; By the equal-volume water washing of pectin stoste, then centrifugation, merging filtrate, the filtrate after merging is concentrated into 1/3 of concentrated front extracting liquid volume, namely cools fast; In fructose concentrating liquid, add the hydrogen peroxide of fructose concentrating liquid quality meter 1.0%, decolour under room temperature 40 min, and then adding 2 times of concentration is the ethanol of 95%, leaves standstill 30min, filters; In filter cake, add 75% washing with alcohol of 2 times of weight again, repeatedly wash 4 times, filter, with the washing with alcohol dehydration of 95%, after dry, pulverize, be pectin powder finished product.Collect filtrate, vacuum concentration, reclaim ethanol and recycle.
The extraction yield of the present embodiment pectin is 22.6%, weight loss on drying 7.2%, total galacturonic acid 70.3%, gamma value 71.0%.
Embodiment 3
Lemon peel slag put in the water of tare weight 2.3 times, heated and boiled 5 min, dry, pulverize; The lemon peel slag of pulverizing is mixed by weight the citric acid one Sodium phosphate dibasic damping fluid that 1:25 and pH is 7, then adds and slowly stir with lemon peel slag weighing scale 0.1% ficin, at 70 DEG C, be incubated extraction 5 h; Pectin extracting soln centrifugation is obtained pectin stoste; By the equal-volume water washing of pectin stoste, then centrifugation, merging filtrate, the filtrate after merging is concentrated into 1/3 of concentrated front extracting liquid volume, namely cools fast; In fructose concentrating liquid, add the hydrogen peroxide of fructose concentrating liquid quality meter 2.0%, decolour under room temperature 50 min, and then adding 2 times of concentration is the ethanol of 95%, leaves standstill 60min, filters; In filter cake, add 75% washing with alcohol of 2.3 times of weight again, repeatedly wash 5 times, filter, with the washing with alcohol dehydration of 95%, after dry, pulverize, be pectin powder finished product.Collect filtrate, vacuum concentration, reclaim ethanol and recycle.
The extraction yield of the present embodiment pectin is 26.6%, weight loss on drying 7.6%, total galacturonic acid 70.4%, gamma value 60.2%.
Embodiment 4
Lemon peel slag put in the water of tare weight 2.6 times, heated and boiled 10 min, dry, pulverize; The lemon peel slag of pulverizing is mixed by weight the citric acid one Sodium phosphate dibasic damping fluid that 1:30 and pH is 7.5, then adds and slowly stir with lemon peel slag weighing scale 0.3% ficin, at 55 DEG C, be incubated extraction 2 h; Pectin extracting soln centrifugation is obtained pectin stoste; By the equal-volume water washing of pectin stoste, then centrifugation, merging filtrate, the filtrate after merging is concentrated into 1/4 of concentrated front extracting liquid volume, namely cools fast; In fructose concentrating liquid, add the hydrogen peroxide of fructose concentrating liquid quality meter 2.0%, decolour under room temperature 50 min, and then adding 1 times of concentration is the ethanol of 95%, leaves standstill 60min, filters; In filter cake, add 75% washing with alcohol of 2.3 times of weight again, repeatedly wash 4 times, filter, with the washing with alcohol dehydration of 95%, after dry, pulverize, be pectin powder finished product.Collect filtrate, vacuum concentration, reclaim ethanol and recycle.
The extraction yield of the present embodiment pectin is 31.4%, weight loss on drying 7.8%, total galacturonic acid 66.0%, gamma value 63.4%.
Embodiment 5
Lemon peel slag put in the water of tare weight 3 times, heated and boiled 8 min, dry, pulverize; The lemon peel slag of pulverizing is mixed by weight the citric acid one Sodium phosphate dibasic damping fluid that 1:30 and pH is 4, then adds and slowly stir with lemon peel slag weighing scale 0.2% hemicellulase, at 60 DEG C, be incubated extraction 2 h; Pectin extracting soln centrifugation is obtained pectin stoste; By the equal-volume water washing of pectin stoste, then centrifugation, merging filtrate, the filtrate after merging is concentrated into 1/2 of concentrated front extracting liquid volume, namely cools fast; In fructose concentrating liquid, add the hydrogen peroxide of fructose concentrating liquid quality meter 2.0%, decolour under room temperature 60 min, and then adding 1.5 times of concentration is the ethanol of 95%, leaves standstill 40min, filters; In filter cake, add 75% washing with alcohol of 2 times of weight again, repeatedly wash 4 times, filter, with the washing with alcohol dehydration of 95%, after dry, pulverize, be pectin powder finished product.Collect filtrate, vacuum concentration, reclaim ethanol and recycle.
The extraction yield of the present embodiment pectin is 23.6%, weight loss on drying 7.4%, total galacturonic acid 71.0%, gamma value 65.8%.
Embodiment 6
Lemon peel slag put in the water of tare weight 2 times, heated and boiled 7 min, dry, pulverize; The lemon peel slag of pulverizing is mixed by weight citric acid one sodium citrate buffer solution that 1:15 and pH is 8, then adds and slowly stir with lemon peel slag weighing scale 0.25% bromeline, at 45 DEG C, be incubated extraction 3 h; Pectin extracting soln centrifugation is obtained pectin stoste; By the equal-volume water washing of pectin stoste, then centrifugation, merging filtrate, the filtrate after merging is concentrated into 1/2 of concentrated front extracting liquid volume, namely cools fast; In fructose concentrating liquid, add the hydrogen peroxide of fructose concentrating liquid quality meter 2.0%, decolour under room temperature 60 min, and then adding 1.5 times of concentration is the ethanol of 95%, leaves standstill 40min, filters; In filter cake, add 75% washing with alcohol of 2.6 times of weight again, repeatedly wash 3 times, filter, with the washing with alcohol dehydration of 95%, after dry, pulverize, be pectin powder finished product.Collect filtrate, vacuum concentration, reclaim ethanol and recycle.
The extraction yield of the present embodiment pectin is 26.4%, weight loss on drying 7.3%, total galacturonic acid 67.8%, gamma value 68.4%.
Embodiment 7
Lemon peel slag put in the water of tare weight 2 times, heated and boiled 7 min, dry, pulverize; The lemon peel slag of pulverizing is mixed by weight citric acid one sodium citrate buffer solution that 1:20 and pH is 6.5, then adds and slowly stir with lemon peel slag weighing scale 0.1% papoid, at 65 DEG C, be incubated extraction 1 h; Pectin extracting soln centrifugation is obtained pectin stoste; By the equal-volume water washing of pectin stoste, then centrifugation, merging filtrate, the filtrate after merging is concentrated into 1/2 of concentrated front extracting liquid volume, namely cools fast; In fructose concentrating liquid, add the hydrogen peroxide of fructose concentrating liquid quality meter 2.0%, decolour under room temperature 55 min, and then adding 3 times of concentration is the ethanol of 95%, leaves standstill 50min, filters; In filter cake, add 75% washing with alcohol of 2.6 times of weight again, repeatedly wash 3 times, filter, with the washing with alcohol dehydration of 95%, after dry, pulverize, be pectin powder finished product.Collect filtrate, vacuum concentration, reclaim ethanol and recycle.
The extraction yield of the present embodiment pectin is 29.4%, weight loss on drying 7.1%, total galacturonic acid 67.6%, gamma value 65.5%.
Embodiment 8
Lemon peel slag put in the water of tare weight 2.6 times, heated and boiled 8 min, dry, pulverize; Mixed by weight the citric acid one Sodium phosphate dibasic damping fluid that 1:20 and pH is 8 by the lemon peel slag of pulverizing, then add and slowly stir with lemon peel slag weighing scale 0.05% cellulase and 0.05% papoid, at 55 DEG C, 2h is extracted in insulation; Pectin extracting soln centrifugation is obtained pectin stoste; By the equal-volume water washing of pectin stoste, then centrifugation, merging filtrate, the filtrate after merging is concentrated into 1/3 of concentrated front extracting liquid volume, namely cools fast; In fructose concentrating liquid, add the hydrogen peroxide of fructose concentrating liquid quality meter 2.0%, decolour under room temperature 50 min, and then adding 2 times of concentration is the ethanol of 95%, leaves standstill 50min, filters; In filter cake, add 75% washing with alcohol of 3 times of weight again, repeatedly wash 3 times, filter, with the washing with alcohol dehydration of 95%, after dry, pulverize, be pectin powder finished product.Collect filtrate, vacuum concentration, reclaim ethanol and recycle.
The extraction yield of the present embodiment pectin is 33.4%, weight loss on drying 7.5%, total galacturonic acid 65.4%, gamma value 64.0%.
Embodiment 9
Lemon peel slag put in the water of tare weight 3 times, heated and boiled 10 min, dry, pulverize; Mixed by weight citric acid one sodium citrate buffer solution that 1:20 and pH is 6 by the lemon peel slag of pulverizing, then add and slowly stir with lemon peel slag weighing scale 0.05% hemicellulase and 0.05% ficin, at 60 DEG C, 2.5h is extracted in insulation; Pectin extracting soln centrifugation is obtained pectin stoste; By the equal-volume water washing of pectin stoste, then centrifugation, merging filtrate, the filtrate after merging is concentrated into 1/2 of concentrated front extracting liquid volume, namely cools fast; In fructose concentrating liquid, add the hydrogen peroxide of fructose concentrating liquid quality meter 2.0%, decolour under room temperature 50 min, and then adding 2 times of concentration is the ethanol of 95%, leaves standstill 60min, filters; In filter cake, add 75% washing with alcohol of 3 times of weight again, repeatedly wash 3 times, filter, with the washing with alcohol dehydration of 95%, after dry, pulverize, be pectin powder finished product.Collect filtrate, vacuum concentration, reclaim ethanol and recycle.
The extraction yield of the present embodiment pectin is 37.6%, weight loss on drying 7.6%, total galacturonic acid 65.1%, gamma value 62.4%.
Embodiment 10
Lemon peel slag put in the water of tare weight 3 times, heated and boiled 10 min, dry, pulverize; Mixed by weight the citric acid one Sodium phosphate dibasic damping fluid that 1:25 and pH is 7 by the lemon peel slag of pulverizing, then add and slowly stir with lemon peel slag weighing scale 0.1% papoid and 0.1% bromeline, at 50 DEG C, 3h is extracted in insulation; Pectin extracting soln centrifugation is obtained pectin stoste; By the equal-volume water washing of pectin stoste, then centrifugation, merging filtrate, the filtrate after merging is concentrated into 1/3 of concentrated front extracting liquid volume, namely cools fast; In fructose concentrating liquid, add the hydrogen peroxide of fructose concentrating liquid quality meter 3.0%, decolour under room temperature 50 min, and then adding 2 times of concentration is the ethanol of 95%, leaves standstill 60min, filters; In filter cake, add 75% washing with alcohol of 3 times of weight again, repeatedly wash 3 times, filter, with the washing with alcohol dehydration of 95%, after dry, pulverize, be pectin powder finished product.Collect filtrate, vacuum concentration, reclaim ethanol and recycle.
The extraction yield of the present embodiment pectin is 36.9%, weight loss on drying 7.7%, total galacturonic acid 65.4%, gamma value 61.3%.
Claims (3)
1. utilize enzyme process from lemon peel slag, extract a method for pectin, processing step and the condition of the method are as follows:
1) lemon peel slag is put in the water of tare weight 2 ~ 3 times, heated and boiled 5 ~ 10min, with clear water rinsing to colourless, dry, pulverize;
2) by the damping fluid mixing that the lemon peel slag of pulverizing is 4 ~ 8 by weight 1:15 ~ 1:30 and pH, then add and slowly stir with lemon peel slag weighing scale 0.01 ~ 0.3% zymin, at 40 ~ 70 DEG C, be incubated extraction 1 ~ 5h;
3) by pectin extracting soln centrifugation, gained pectin stoste is concentrated into 1/4 ~ 1/2 of concentrated front extracting liquid volume, namely cools fast;
4) add in fructose concentrating liquid in the hydrogen peroxide of fructose concentrating liquid quality 0.5 ~ 3%, decolour under room temperature 30 ~ 60min, and then adding 1 ~ 3 times of concentration is the ethanol of 95%, leaves standstill 30 ~ 60min, filter, in filter cake, add 75% washing with alcohol of 2 ~ 3 times of weight again, repeatedly wash 3 ~ 5 times, filter, washing with alcohol is dewatered, pectin powder finished product is after dry, pulverize
Zymin wherein used is papoid, at least one in bromeline and ficin or any one mixed enzyme formed with cellulase or hemicellulase in papoid, bromeline and ficin.
2. the method utilizing enzyme process to extract pectin from lemon peel slag according to claim 1, the method damping fluid used is that citric acid one citric acid is received or citric acid one Sodium phosphate dibasic composition.
3. the method utilizing enzyme process to extract pectin from lemon peel slag according to claim 1 and 2, the lemon peel slag pulverized in the method is the damping fluid mixing of 6 ~ 8 by weight 1:20 ~ 1:30 and pH.
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| CN111543629A (en) * | 2020-04-07 | 2020-08-18 | 杭州莱恩纳尔生物科技有限公司 | Method for simultaneously preparing pectin, fruit vinegar, ferment, probiotics and carbon black from peel residues |
| CN113712189B (en) * | 2021-08-27 | 2024-05-17 | 南宁学院 | Biological enzyme extraction process of pectin in papaya peel |
| CN115417936B (en) * | 2022-10-23 | 2023-04-07 | 华南理工大学 | Method for extracting pectin from waste beet pulp after sugar production by double-enzyme method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101507482A (en) * | 2009-03-26 | 2009-08-19 | 四川省食品发酵工业研究设计院 | Low methoxyi pectin preparation technique using orange peel |
| CN102161713A (en) * | 2011-03-24 | 2011-08-24 | 铜川市耀州爱普乐生物科技有限公司 | Method for continuously preparing high-purity pectin by using enzymolysis-ultrafiltration concentration-spray drying process |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101507482A (en) * | 2009-03-26 | 2009-08-19 | 四川省食品发酵工业研究设计院 | Low methoxyi pectin preparation technique using orange peel |
| CN102161713A (en) * | 2011-03-24 | 2011-08-24 | 铜川市耀州爱普乐生物科技有限公司 | Method for continuously preparing high-purity pectin by using enzymolysis-ultrafiltration concentration-spray drying process |
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