CN108148149A - A kind of method of pectin in Enzymatic Extraction orange peel - Google Patents
A kind of method of pectin in Enzymatic Extraction orange peel Download PDFInfo
- Publication number
- CN108148149A CN108148149A CN201611102318.6A CN201611102318A CN108148149A CN 108148149 A CN108148149 A CN 108148149A CN 201611102318 A CN201611102318 A CN 201611102318A CN 108148149 A CN108148149 A CN 108148149A
- Authority
- CN
- China
- Prior art keywords
- orange peel
- pectin
- enzyme
- mentioned
- trapped fluid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0045—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid alpha-D-Galacturonans, e.g. methyl ester of (alpha-1,4)-linked D-galacturonic acid units, i.e. pectin, or hydrolysis product of methyl ester of alpha-1,4-linked D-galacturonic acid units, i.e. pectinic acid; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
Abstract
The invention discloses a kind of methods of pectin in Enzymatic Extraction orange peel, include the following steps：1) it is 0.5~2mm to crush orange peel to its grain size；2) by orange peel under 80~90 DEG C of temperature conditions 5~15min of washing by soaking；3) by treated orange peel and 8~10 times of citric acid sodium citrate buffer mixing, enzyme reaction tank is put into；4) start enzyme reaction tank to be heated, put into mixing enzyme preparation；5) after above-mentioned enzyme digestion reaction after filtering, enzymolysis liquid is collected using steam enzyme deactivation；6) enzymolysis liquid of above-mentioned collection is continuously filtered using high speed tube centrifuge, removes tiny orange peel, collects trapped fluid；7) trapped fluid of the above-mentioned collection of refined filtration is continuously filtered using UF membrane/concentrator, removes remaining orange peel, collects trapped fluid；8) the low temperature thin layer drying equipment of the pectin solution after above-mentioned concentrating and impurity removing is handled to get to pectin sample, the present invention is environmentally friendly, efficient, and simple for process, easy to operate, quality of finished product good reduces energy consumption.
Description
Technical field
The present invention relates to pectin production technical field, the method for pectin in specially a kind of Enzymatic Extraction orange peel.
Background technology
Orange is either eaten raw or processes, and skin is its Main By product, accounts for about the 20%~40% of fruit weight, in skin
Pectin content is the important source material of commercialization pectin up to 20%~30%.At present, it is domestic to be used to orange peel except a few peoples produce perfume (or spice)
It outside essential oil and feed, is largely simply discarded or fills, not only serious waste resource, more causes environment huge danger
Evil.Pectin is extracted from orange peel not only has very big economic value, but also the comprehensive utilization ratio to improving resource, reduces environment
Pollution keeps the sustainable development of China's Citrus Industry to be of great significance.Pectin is natural food additives, not only can be with
As stabilizer, emulsifier and thickener, but also there is hypoglycemic, reducing blood lipid and norcholesterol, therefore to pectin
The exploitation of production method has great importance.
Current main preparation method has acidic ethanol method, alkalization method, amidation method, microwave method, microbial method (mainly black song
It is mould) etc..Although there are many method, all different degrees of there are highly energy-consuming, high pollution, unstable product quality, production cycle are long
The shortcomings of.Such as acid hydrolyzation, this is the most common method of industrial production pectin.Water can be changed by being heated in diluted acid according to pectin
The principle of dissolubility pectin, raw material is crushed, rinse after add in suitable water, pH value of solution is adjusted to 2.0 or so with acid, 80 DEG C-
Heating extracting a period of time, most of pectin extraction is come out in the range of 90 DEG C.Mostly acid used is sulfuric acid, hydrochloric acid, phosphoric acid etc.
Inorganic acid, in order to improve the color and luster of pectin finished product, it is also possible to sulfurous acid.At present extracting method the problem is that：1st, it needs big
Amount is extracted using inorganic acid, and acid solution that treated becomes new pollution sources；2nd, local hydrolysis easily occurs for pectin molecule, causes
Pectin molecule amount reduces, and so as to influence the yield of pectin and quality, current yield can only be up to 10% or so；3rd, acid system condition
Under, influence of each operating parameter to final pectin is complicated, so as to which the control for making result between different process generates difficult, causes entire
The increasing of technique controlling difficulty.
Invention content
The purpose of the present invention is to provide a kind of methods of pectin in Enzymatic Extraction orange peel, and pectin is extracted using this method,
Pectin is hardly degraded, and products obtained therefrom molecular mass is high, and is avoided using inorganic acid and reduced organic solvent in extraction
Application, reduce environmental pollution, to solve the problems mentioned in the above background technology.
To achieve the above object, the present invention provides following technical solution：
The method of pectin, includes the following steps in a kind of Enzymatic Extraction orange peel：
1) it is 0.5~2mm to crush orange peel to its grain size；
2) by orange peel under 80~90 DEG C of temperature conditions 5~15min of washing by soaking, remove soluble sugar, impurity, simultaneously
The enzyme of passivation itself, is then dried；
3) by treated orange peel and 8~10 times of citric acid-sodium citrate buffer mixing, enzyme reaction is put into
Tank adds the orange peel that a certain amount of buffer solution flushing is attached at tank skin, stirs and evenly mixs；
4) start enzyme reaction tank to be heated, put into mixing enzyme preparation, additive amount is 1%~5%, in 40~60 DEG C of temperature
Under degree, 500r/min rotating speeds, reaction is continuously stirred 1~3 hour；
5) after above-mentioned enzyme digestion reaction, while hot with after screen filtration, enzymolysis liquid is collected using steam 5~20min of enzyme deactivation；
6) enzymolysis liquid of above-mentioned collection is continuously filtered using high speed tube centrifuge, removes tiny orange peel, collects retention
Liquid；
7) trapped fluid of the above-mentioned collection of refined filtration is continuously filtered using UF membrane/concentrator, removes remaining orange peel, is collected
Trapped fluid, i.e., 2%~5% concentration pectin solution；
8) the low temperature thin layer drying equipment of the pectin solution after above-mentioned concentrating and impurity removing is handled, adjusts heating temperature to 60 DEG C
~90 DEG C, material web is opened, with distributing device uniform distribution, fabric thickness 1.5mm~2mm adjusts material web movement rate, it is ensured that
Material heating time is 15min~30min, collects dried material, is crushed after cooling to get to pectin sample.
As further embodiment of the present invention, mixing enzyme preparation in the step 4) by cellulase, hemicellulase,
One or more of protopectinase, polygalacturonase are made.
Compared with prior art, the beneficial effects of the invention are as follows：Entire technique does not use any organic reagent and soda acid object
Matter, it is environmentally friendly, efficient, it is simple for process, it is easy to operate；The enzyme preparation used in the present invention, has efficient specificity, and gained pectin is several
It is not degraded, molecular mass is big, quality of finished product good；Using low temperature thin layer drying technology in the present invention, pectin is effectively improved
Final product quality reduces energy consumption.
Specific embodiment
The technical solution in the embodiment of the present invention is clearly and completely described below, it is clear that described embodiment
Only part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, the common skill in this field
Art personnel all other embodiments obtained without making creative work belong to the model that the present invention protects
It encloses.
Embodiment 1：
1) it is 2mm to crush orange peel to its grain size；
2) by orange peel under 80 DEG C of temperature conditions washing by soaking 15min, remove soluble sugar, impurity, while be passivated this
The enzyme of body, is then dried；
3) by treated orange peel and 9 times of citric acid-sodium citrate buffer mixing, enzyme reaction tank is put into,
It adds 1 times of buffer solution and rinses the orange peel for being attached at tank skin, stir and evenly mix；
4) start enzyme reaction tank to be heated, put into mixing enzyme preparation, additive amount 4%, in 50 DEG C of temperature, 500r/min
Under rotating speed, reaction 2.5 hours is continuously stirred；
5) after above-mentioned enzyme digestion reaction, while hot with after screen filtration, enzymolysis liquid is collected using steam enzyme deactivation 10min；
6) enzymolysis liquid of above-mentioned collection is continuously filtered using high speed tube centrifuge, removes tiny orange peel, collects retention
Liquid；
7) trapped fluid of the above-mentioned collection of refined filtration is continuously filtered using UF membrane/concentrator, removes remaining orange peel, is collected
Trapped fluid, i.e., 4.5% concentration pectin solution；
8) the low temperature thin layer drying equipment of the pectin solution after above-mentioned concentrating and impurity removing is handled, adjusts heating temperature to 75
DEG C, material web is opened, with distributing device uniform distribution, fabric thickness 1.5mm~2mm adjusts material web movement rate, it is ensured that object
Material heating time is 25min, collects dried material, is crushed after cooling to get to pectin sample.Wherein, in the step 4)
Mixing enzyme preparation is made of one or more of cellulase, hemicellulase, protopectinase, polygalacturonase.
Present example final result is：Pectin yield is 14.7%, average molecular weight 180kDa.
Embodiment 2：
1) it is 1mm to crush orange peel to its grain size；
2) by orange peel under 85 DEG C of temperature conditions washing by soaking 15min, remove soluble sugar, impurity, while be passivated this
The enzyme of body, is then dried；
3) by treated orange peel and 8.5 times of citric acid-sodium citrate buffer mixing, enzyme reaction is put into
Tank adds 1.5 times of buffer solution and rinses the orange peel for being attached at tank skin, stirs and evenly mixs；
4) start enzyme reaction tank to be heated, put into mixing enzyme preparation, additive amount 5%, in 45 DEG C of temperature, 500r/min
Under rotating speed, reaction 3 hours is continuously stirred；
5) after above-mentioned enzyme digestion reaction, while hot with after screen filtration, enzymolysis liquid is collected using steam enzyme deactivation 15min；
6) enzymolysis liquid of above-mentioned collection is continuously filtered using high speed tube centrifuge, removes tiny orange peel, collects retention
Liquid；
7) trapped fluid of the above-mentioned collection of refined filtration is continuously filtered using UF membrane/concentrator, removes remaining orange peel, is collected
Trapped fluid, i.e., 5% concentration pectin solution；
8) the low temperature thin layer drying equipment of the pectin solution after above-mentioned concentrating and impurity removing is handled, adjusts heating temperature to 90
DEG C, material web is opened, with distributing device uniform distribution, fabric thickness 1.5mm~2mm adjusts material web movement rate, it is ensured that material
Heating time is 15min, collects dried material, is crushed after cooling to get to pectin sample.Wherein, it is mixed in the step 4)
Synthase preparation is made of one or more of cellulase, hemicellulase, protopectinase, polygalacturonase.
Present example final result is：Pectin yield is 17.3%, average molecular weight 230kDa.
Embodiment 3：
1) it is 1.5mm to crush orange peel to its grain size；
2) by orange peel under 90 DEG C of temperature conditions washing by soaking 10min, remove soluble sugar, impurity, while be passivated this
The enzyme of body, is then dried；
3) by treated orange peel and 8 times of citric acid-sodium citrate buffer mixing, enzyme reaction tank is put into,
It adds 2 times of buffer solution and rinses the orange peel for being attached at tank skin, stir and evenly mix；
4) start enzyme reaction tank to be heated, put into mixing enzyme preparation, additive amount 3%, in 55 DEG C of temperature, 500r/min
Under rotating speed, reaction 2 hours is continuously stirred；
5) after above-mentioned enzyme digestion reaction, while hot with after screen filtration, enzymolysis liquid is collected using steam enzyme deactivation 5min；
6) enzymolysis liquid of above-mentioned collection is continuously filtered using high speed tube centrifuge, removes tiny orange peel, collects retention
Liquid；
7) trapped fluid of the above-mentioned collection of refined filtration is continuously filtered using UF membrane/concentrator, removes remaining orange peel, is collected
Trapped fluid, i.e., 4% concentration pectin solution；
8) the low temperature thin layer drying equipment of the pectin solution after above-mentioned concentrating and impurity removing is handled, adjusts heating temperature to 80
DEG C, material web is opened, with distributing device uniform distribution, fabric thickness 1.5mm~2mm adjusts material web movement rate, it is ensured that material
Heating time is 20min, collects dried material, is crushed after cooling to get to pectin sample.Wherein, it is mixed in the step 4)
Synthase preparation is made of one or more of cellulase, hemicellulase, protopectinase, polygalacturonase.
Present example final result is：Pectin yield is 13.3%, average molecular weight 220kDa.
It is obvious to a person skilled in the art that the present invention is not limited to the details of above-mentioned exemplary embodiment, Er Qie
In the case of without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, the present embodiments are to be considered as illustrative and not restrictive, and the scope of the present invention is by appended power
Profit requirement rather than above description limit, it is intended that all by what is fallen within the meaning and scope of the equivalent requirements of the claims
Variation is included within the present invention.
Claims (2)
1. a kind of method of pectin in Enzymatic Extraction orange peel, it is characterised in that include the following steps：
1) it is 0.5~2mm to crush orange peel to its grain size；
2) by orange peel, 5~15min of washing by soaking, the soluble sugar of removal, impurity are passivated simultaneously under 80~90 DEG C of temperature conditions
The enzyme of itself, is then dried；
3) by treated orange peel and 8~10 times of citric acid-sodium citrate buffer mixing, enzyme reaction tank is put into,
The orange peel that a certain amount of buffer solution flushing is attached at tank skin is added, is stirred and evenly mixed；
4) start enzyme reaction tank heated, put into mixing enzyme preparation, additive amount be 1%~5%, 40~60 DEG C of temperature,
Under 500r/min rotating speeds, reaction is continuously stirred 1~3 hour；
5) after above-mentioned enzyme digestion reaction, while hot with after screen filtration, enzymolysis liquid is collected using steam 5~20min of enzyme deactivation；
6) enzymolysis liquid of above-mentioned collection is continuously filtered using high speed tube centrifuge, removes tiny orange peel, collects trapped fluid；
7) trapped fluid of the above-mentioned collection of refined filtration is continuously filtered using UF membrane/concentrator, removes remaining orange peel, collects retention
Liquid, i.e., 2%~5% concentration pectin solution；
8) the low temperature thin layer drying equipment of the pectin solution after above-mentioned concentrating and impurity removing is handled, adjusts heating temperature to 60 DEG C~90
DEG C, material web is opened, with distributing device uniform distribution, fabric thickness 1.5mm~2mm adjusts material web movement rate, it is ensured that material
Heating time is 15min~30min, collects dried material, is crushed after cooling to get to pectin sample.
2. the method for pectin in a kind of Enzymatic Extraction orange peel according to claim 1, it is characterised in that：In the step 4)
Mixing enzyme preparation be made of one or more of cellulase, hemicellulase, protopectinase, polygalacturonase.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611102318.6A CN108148149A (en) | 2016-12-05 | 2016-12-05 | A kind of method of pectin in Enzymatic Extraction orange peel |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611102318.6A CN108148149A (en) | 2016-12-05 | 2016-12-05 | A kind of method of pectin in Enzymatic Extraction orange peel |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108148149A true CN108148149A (en) | 2018-06-12 |
Family
ID=62469728
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611102318.6A Pending CN108148149A (en) | 2016-12-05 | 2016-12-05 | A kind of method of pectin in Enzymatic Extraction orange peel |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108148149A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103232555A (en) * | 2013-05-16 | 2013-08-07 | 内江师范学院 | Method for extracting pectin from lemon peel residues by utilizing enzymatic method |
| CN103641930A (en) * | 2013-11-27 | 2014-03-19 | 威海市桢昊生物技术有限公司 | Method for extraction of pectin from pineapple peels and residue by enzymatic process |
-
2016
- 2016-12-05 CN CN201611102318.6A patent/CN108148149A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103232555A (en) * | 2013-05-16 | 2013-08-07 | 内江师范学院 | Method for extracting pectin from lemon peel residues by utilizing enzymatic method |
| CN103641930A (en) * | 2013-11-27 | 2014-03-19 | 威海市桢昊生物技术有限公司 | Method for extraction of pectin from pineapple peels and residue by enzymatic process |
Non-Patent Citations (6)
| Title |
|---|
| 李再新主编: "《制药分离工程实验》", 30 June 2016, 西南交通大学出版社 * |
| 汪多仁编著: "《有机食品营养强化剂》", 31 August 2008, 科学技术文献出版社 * |
| 赵黎明主编: "《膜分离技术在食品发酵工业中的应用》", 31 July 2011, 中国纺织出版社 * |
| 郝利平等主编: "《食品添加剂》", 31 July 2016, 中国农业大学出版社 * |
| 阮尚全等: ""酶解-柠檬酸提取塔罗科血橙皮中的果胶及理化性质研究"，", 《食品工业科技》 * |
| 韩冬梅: ""酶法制取橙皮果胶的研究"", 《食品工业科技》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101224022B (en) | Processing method for simultaneously preparing sesame oil and protein by water enzymatic extraction | |
| CN104086667A (en) | Method for preparing pectin from citrus fruit peel residues by employing ultrasonic-assisted extraction | |
| CN102747637B (en) | Process for producing cellulose by residues of pomace after pectin extraction | |
| CN104558238B (en) | Process for extracting sodium alginate | |
| CN104223125A (en) | Method for preparing DF (Dietary Fiber) of potato pulp | |
| CN102351944B (en) | Method for extracting alfalfa leaf protein by ultrasonic-assisted enzyme process | |
| CN102559808B (en) | Method for preparing xylo-oligosaccharide by using corn stalk cores | |
| CN106349405A (en) | Method for extracting pectin from shaddock peel through enzymolysis and ultrasonic waves | |
| CN106282266B (en) | Method for preparing enteromorpha prolifera oligosaccharide by using naringinase | |
| CN102286111B (en) | Process for extracting pectin from pear skin at normal temperature | |
| CN104694587A (en) | Method for producing lactic acid from bagasse | |
| CN101153306B (en) | Method for producing tangerine peel low methoxy group pectin by using pectinase | |
| CN103636917B (en) | Utilize Hydrodynamic Cavitation Technology from rice residue, extract the method for rice protein | |
| CN101213994A (en) | Method for using ultrasound wave auxiliary enzyme to extract apple seed oil | |
| CN105249481A (en) | Method for preparing high-swelling-property dietary fibers from residues generated after folium mori extraction | |
| CN109485746A (en) | A method of extracting plant pectin from hemp hemp skin | |
| CN108998491A (en) | A method of extracting protein in the peony seeds dregs of rice | |
| CN102399303A (en) | Extraction method of pectin | |
| CN102532344A (en) | Method for extracting orange peel pectin by softening orange peel and inactivating pectinesterase | |
| CN100532396C (en) | Process for preparing high purity pectin by using apple pomace | |
| CN102181076B (en) | Method for cleanly preparing starch containing abundant flavones and comprehensively utilizing radix puerariae resource | |
| CN108148149A (en) | A kind of method of pectin in Enzymatic Extraction orange peel | |
| CN101805416B (en) | Preparation method of canna edulis ker pectin | |
| CN103397061B (en) | Method for treating cellulose | |
| CN102210458B (en) | Method for preparing algal residue dietary fiber |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180612 |