CN103221105B - 生物聚合物色谱的系统和工艺 - Google Patents

生物聚合物色谱的系统和工艺 Download PDF

Info

Publication number
CN103221105B
CN103221105B CN201180057837.4A CN201180057837A CN103221105B CN 103221105 B CN103221105 B CN 103221105B CN 201180057837 A CN201180057837 A CN 201180057837A CN 103221105 B CN103221105 B CN 103221105B
Authority
CN
China
Prior art keywords
column
biopolymer
tank
storage tank
outlet
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201180057837.4A
Other languages
English (en)
Chinese (zh)
Other versions
CN103221105A (zh
Inventor
M.哈尔
K.拉基
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cytiva Sweden AB
Original Assignee
GE Healthcare Bio Sciences Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GE Healthcare Bio Sciences Corp filed Critical GE Healthcare Bio Sciences Corp
Publication of CN103221105A publication Critical patent/CN103221105A/zh
Application granted granted Critical
Publication of CN103221105B publication Critical patent/CN103221105B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
    • B01D15/1864—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns
    • B01D15/1871—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns placed in series
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/24—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the treatment of the fractions to be distributed
    • B01D15/242—Intermediate storage of effluents
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3847—Multimodal interactions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/44—Flow patterns using recycling of the fraction to be distributed
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G01N30/461—Flow patterns using more than one column with serial coupling of separation columns
    • G01N30/465—Flow patterns using more than one column with serial coupling of separation columns with specially adapted interfaces between the columns
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G01N2030/8831—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/42—Flow patterns using counter-current

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Molecular Biology (AREA)
  • Sustainable Development (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
CN201180057837.4A 2010-12-03 2011-12-02 生物聚合物色谱的系统和工艺 Active CN103221105B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
SE1051279-6 2010-12-03
SE1051279 2010-12-03
PCT/SE2011/051468 WO2012074481A1 (en) 2010-12-03 2011-12-02 System and process for biopolymer chromatography

Publications (2)

Publication Number Publication Date
CN103221105A CN103221105A (zh) 2013-07-24
CN103221105B true CN103221105B (zh) 2015-11-25

Family

ID=46172172

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201180057837.4A Active CN103221105B (zh) 2010-12-03 2011-12-02 生物聚合物色谱的系统和工艺

Country Status (5)

Country Link
US (1) US20130248451A1 (enExample)
EP (1) EP2675540B1 (enExample)
JP (1) JP5802760B2 (enExample)
CN (1) CN103221105B (enExample)
WO (1) WO2012074481A1 (enExample)

Families Citing this family (52)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10967298B2 (en) 2012-03-15 2021-04-06 Flodesign Sonics, Inc. Driver and control for variable impedence load
US9458450B2 (en) 2012-03-15 2016-10-04 Flodesign Sonics, Inc. Acoustophoretic separation technology using multi-dimensional standing waves
US9950282B2 (en) 2012-03-15 2018-04-24 Flodesign Sonics, Inc. Electronic configuration and control for acoustic standing wave generation
US10704021B2 (en) 2012-03-15 2020-07-07 Flodesign Sonics, Inc. Acoustic perfusion devices
EP2888595B1 (en) * 2012-08-22 2021-03-17 Cytiva Sweden AB Versatile rotary valve
CN110075568A (zh) 2012-08-24 2019-08-02 通用电气健康护理生物科学股份公司 控制液相色谱系统的系统和方法
US9322014B1 (en) * 2012-11-28 2016-04-26 Sandia Corporation Multiplexed microfluidic approach for nucleic acid enrichment
AU2014226126B2 (en) 2013-03-08 2019-02-14 Genzyme Corporation Continuous purification of therapeutic proteins
CN103157295B (zh) * 2013-03-19 2014-12-24 利穗科技(苏州)有限公司 一种双柱循环色谱系统
US10099156B2 (en) † 2013-04-08 2018-10-16 Chromacon Ag Chromatographic purification method
CN105939767B (zh) 2014-01-08 2018-04-06 弗洛设计声能学公司 具有双声电泳腔的声电泳装置
TWI709570B (zh) 2014-01-17 2020-11-11 美商健臻公司 無菌層析法及製法
TWI709569B (zh) 2014-01-17 2020-11-11 美商健臻公司 無菌層析樹脂及其用於製造方法的用途
GB201401010D0 (en) 2014-01-21 2014-03-05 Nat Nuclear Lab Ltd Improved separation apparatus and method
US11267869B2 (en) * 2014-02-07 2022-03-08 Cmc Biologics A/S Chromatography system and method for capturing a biopolymer
JP6612242B2 (ja) * 2014-02-14 2019-11-27 ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ 自動化された多段階精製システム及び多段階クロマトグラフィー精製方法
NZ724826A (en) 2014-04-16 2022-07-29 Cmc Biologics As A high cell density fill and draw fermentation process
WO2016098169A1 (ja) * 2014-12-15 2016-06-23 株式会社島津製作所 液体クロマトグラフ
US11938418B2 (en) 2014-12-18 2024-03-26 Cytiva Sweden Ab Automated chromatography column switching control based on pressure detection
CN104614458B (zh) * 2015-01-21 2016-06-22 柳仁民 一种带多通阀的循环制备高效液相色谱仪
US11708572B2 (en) 2015-04-29 2023-07-25 Flodesign Sonics, Inc. Acoustic cell separation techniques and processes
US11021699B2 (en) 2015-04-29 2021-06-01 FioDesign Sonics, Inc. Separation using angled acoustic waves
US11377651B2 (en) 2016-10-19 2022-07-05 Flodesign Sonics, Inc. Cell therapy processes utilizing acoustophoresis
EP3037513A1 (de) * 2015-05-13 2016-06-29 Bayer Technology Services GmbH Verfahren zur kontinuierlichen elution eines produktes von chromatographiesäulen
US11474085B2 (en) 2015-07-28 2022-10-18 Flodesign Sonics, Inc. Expanded bed affinity selection
US11459540B2 (en) 2015-07-28 2022-10-04 Flodesign Sonics, Inc. Expanded bed affinity selection
GB201515339D0 (en) 2015-08-28 2015-10-14 Ge Healthcare Bio Sciences Ab A seperation matrix and a method of seperating antibodies
US11099159B2 (en) 2016-03-07 2021-08-24 Waters Technologies Corporation Systems, methods and devices for reducing band dispersion in chromatography
US11085035B2 (en) 2016-05-03 2021-08-10 Flodesign Sonics, Inc. Therapeutic cell washing, concentration, and separation utilizing acoustophoresis
US11214789B2 (en) 2016-05-03 2022-01-04 Flodesign Sonics, Inc. Concentration and washing of particles with acoustics
EP4574227A3 (en) 2016-09-29 2025-09-03 Bio-Rad Laboratories, Inc. Protein-nanoparticle conjugate purification methods
EP3529347A1 (en) 2016-10-19 2019-08-28 Flodesign Sonics, Inc. Affinity cell extraction by acoustics
GB201703116D0 (en) 2017-02-27 2017-04-12 Ge Healthcare Bioprocess R&D Ab A seperation matrix and a method of seperating antibodies
EP3381529A1 (fr) 2017-03-29 2018-10-03 EMD Millipore Corporation Installation de traitement de liquide biologique
EP3381530A1 (fr) 2017-03-29 2018-10-03 EMD Millipore Corporation Installation de traitement de liquide biologique
JP6790963B2 (ja) * 2017-03-30 2020-11-25 株式会社島津製作所 液体クロマトグラフ
CN108854158B (zh) * 2017-05-16 2023-11-07 湖北生物医药产业技术研究院有限公司 树脂柱洗脱液收集装置
GB201710130D0 (en) 2017-06-26 2017-08-09 Ge Healthcare Bioprocess R & D Ab A Method of seperation
JP7333779B2 (ja) * 2017-11-16 2023-08-25 クロマコン アーゲー 循環クロマトグラフィー精製プロセスを監視、評価及び制御する方法
US12029999B2 (en) 2017-11-30 2024-07-09 Corning Incorporated Package for batch chromatography
CN114900773A (zh) 2017-12-14 2022-08-12 弗洛设计声能学公司 声泳系统及其操作方法、控制声换能器及声学系统的方法
SG11202101369XA (en) * 2018-08-17 2021-03-30 Regeneron Pharma Method and chromatography system for determining amount and purity of a multimeric protein
GB202006310D0 (en) 2020-04-29 2020-06-10 Puridify Ltd Preparative chromatography system and method for chromatography separations
CN111957072A (zh) * 2020-09-10 2020-11-20 北京莱伯泰科仪器股份有限公司 不锈钢自密封凝胶净化柱装置
US12458908B2 (en) * 2020-11-30 2025-11-04 PAK Biosolutions Inc. End-to-end continuous purification system
GB202109015D0 (en) * 2021-06-23 2021-08-04 Puridify Ltd A method for purifying a feed in a chromatography system
CN117794628A (zh) * 2021-08-13 2024-03-29 蓝科有限公司 用于从液体混合物中分离分析物的工业方法
EP4486476A1 (en) 2022-03-03 2025-01-08 ChromaCon AG Chromatographic purification method and uses thereof
CN115015458A (zh) * 2022-07-01 2022-09-06 江苏汉邦科技股份有限公司 一种分流色谱系统和利用分流色谱系统制备二十碳五烯酸乙酯的方法
CN116474419B (zh) * 2023-06-09 2025-07-18 焦作市华康糖醇科技有限公司 一种木糖生产连续脱色系统及其使用方法
WO2025031868A1 (en) 2023-08-08 2025-02-13 Cytiva Sweden Ab Multi modular chromatography system
CN119345745B (zh) * 2024-12-23 2025-03-14 成都英德生物医药设备有限公司 一种生产级连续流层析系统及方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101053705A (zh) * 2007-05-24 2007-10-17 江苏汉邦科技有限公司 五分区模拟移动床色谱装置
WO2008064242A2 (en) * 2006-11-22 2008-05-29 Genitope Corporation Chromatography systems comprising single-use components
US20090149638A1 (en) * 2007-10-03 2009-06-11 Ley Arthur C Systems and methods for purifying proteins
CN101687119A (zh) * 2007-06-15 2010-03-31 通用电气健康护理生物科学股份公司 色谱方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US39398A (en) * 1863-08-04 Improvement in blow-pipes
US5683916A (en) * 1988-10-31 1997-11-04 Hemasure Inc. Membrane affinity apparatus and purification methods related thereto
US20060273013A1 (en) * 2004-03-01 2006-12-07 Chin Nien-Hwa L Versatile simulated moving bed systems
US8920645B2 (en) * 2005-12-07 2014-12-30 Tarpon Biosystems Inc. Disposable chromatography valves and system

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008064242A2 (en) * 2006-11-22 2008-05-29 Genitope Corporation Chromatography systems comprising single-use components
CN101053705A (zh) * 2007-05-24 2007-10-17 江苏汉邦科技有限公司 五分区模拟移动床色谱装置
CN101687119A (zh) * 2007-06-15 2010-03-31 通用电气健康护理生物科学股份公司 色谱方法
US20090149638A1 (en) * 2007-10-03 2009-06-11 Ley Arthur C Systems and methods for purifying proteins

Also Published As

Publication number Publication date
EP2675540A4 (en) 2016-12-28
EP2675540B1 (en) 2018-03-21
CN103221105A (zh) 2013-07-24
JP2014505236A (ja) 2014-02-27
US20130248451A1 (en) 2013-09-26
WO2012074481A1 (en) 2012-06-07
JP5802760B2 (ja) 2015-11-04
EP2675540A1 (en) 2013-12-25

Similar Documents

Publication Publication Date Title
EP2675540B1 (en) System and process for biopolymer chromatography
CN101687119B (zh) 色谱方法
CN103180727B (zh) 具有保护柱的色谱系统
CN101340960B (zh) 用于色谱分离的装置
EP3105580B1 (en) Automated multi-step purification system
US10843104B2 (en) System and process for biopolymer chromatography
US20260061337A1 (en) Method in Continuous Chromatography
US20080116122A1 (en) Chromatography systems comprising single-use components
CN101678244A (zh) 用于连续膜吸附的方法和装置
US20170361245A1 (en) Valve Manifolds for Simulated Moving Bed Chromatography
US12616919B2 (en) Optimizing the operation of a chromatography system
US20250369840A1 (en) Sample preparation system and method for preparing a sample using the sample preparation system
CN212999359U (zh) 一种自动超滤膜包装置
US12201922B2 (en) Method for operating a chromatography setup
CN216273870U (zh) 一种全自动密度梯度分离仪与蛋白纯化系统联用装置
CN116367902A (zh) 用于膜色谱的系统

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
CP01 Change in the name or title of a patent holder
CP01 Change in the name or title of a patent holder

Address after: uppsala

Patentee after: Stoivan Sweden Limited

Address before: uppsala

Patentee before: GE HEALTHCARE BIO-SCIENCES AB