CN103202385A - Feeding super probiotic production method - Google Patents

Abstract

The invention relates to a feeding super probiotic production method. The method includes: on the basis of carrying out directed functional screening to different probiotics lactobacilli and probiotics bacilli, selecting three types of lactobacilli with antibacterial, amylase secretory and bile salt hydrolase activities and one type of bacilli, subjecting the lactobacilli and the bacilli to three-stage amplification fermentation and flocculating settling to obtain lactobacilli concentrated slurries and a bacilli concentrated slurry, adding peridium wall materials into the lactobacilli concentrated slurries and the bacilli concentrated slurry, and performing spray drying to obtain respective microcapsule lactobacilli powders and microcapsule bacilli powder; using acid and alkali for treating shrimp shells or crab shells in sequence to prepare chitosan, performing enzymolysis for the chitosan, and controlling enzyme concentration and hydrolysis time to obtain mixed hydrolysate containing oligose, oligosaccharide and polysaccharide in a specific proportion, and concentrating and drying the hydrolysate to obtain complex functional carbohydrate; and uniformly mixing the microcapsule lactobacilli powders and the microcapsule bacilli powder with the complex functional carbohydrate according to a specific proportion so that a novel green feeding super probiotic feed additive product is obtained.

Description

The production method of feeding super probiotic

Technical field

The present invention relates to a kind of production method of feeding super probiotic product, spore bacillus that different benefits is sprouted, benefit lactogenesis acidfast bacilli carries out on the basis of directed functional screening, through strict Combination Design, to have antibiotic, secreting amylase, 3 genera lactobacillus of bile salt hydrolase activity and 1 series bacillus with secretion antibacterial peptide function with contain the special ratios oligosaccharides, compound sugar, the composite functional sugar of polysaccharide is organic assembling in specific proportions, obtain feeding super probiotic product, make it from immunity, little ecology, number of ways such as organism metabolism guarantee livestock birds health, improve Production of Livestock and Poultry power, guarantee product safety, healthy, efficiently, belong to novel green feed addictive production field.

Background technology

In actual production, people often add antibiotic artificially in order to reach certain production and economic aim in feed.Antibiotic has that consumption is few, and cost is low, but the tangible characteristics of action effect, so be subjected to the producer's favor very soon, and be extensive use of aborning.Yet residues of antibiotics, toxic and side effect, drug resistance etc. but are the key factors of harm health of people.Especially in actual production, in order to reach certain economic aim, strengthen using dosage, exceed the time limit to use the phenomenon of (ignoring the restriction of antibiotic withdrawal time) and violated use quite general.Along with the raising of people's living standard and health perception, food-safety problem has obtained extensive concern in the world, forbids using in herding is produced antibiotic cry more and more higher.At present, European Union has prohibited and has used antibiotic as growth accelerator in animal feed, comprises that many countries of China are also progressively banning use of the part antibiotic.Therefore, seek to develop the antibiotic substitute of natural, green and environmental protection, become the emphasis that animal husbandry is paid close attention to.

Food security is to guarantee that people are healthy, improves the important assurance of fitness.Raising along with people's living standard, animal products quantitatively is protected substantially, and people pay close attention to the animal products quality more, calls " quality-assured meat ", green safety products such as " antibiotic-free milks ", particularly in recent years, the outburst of rabid ox disease, bird flu, " swine flu ", aftosa popular, etc., coverd with one deck shade for people's life, people the feared state of mind also occurred to the consumption of livestock products, call more " safety food ".Yet, do not have livestock and poultry " safe feed ", the safe animal products of yet just not knowing where to begin.From the angle analysis of food chain, " feed safety is exactly food security " guarantees animal products safety from the feed source, will be the important measures that ensure meat, eggs and milk based food safety undoubtedly.

Therefore, how under the condition that does not influence ordinary production deduction and exemption and alternative antibiotic, use effective substitute, be the focus that current people pay close attention to, also be the focus of research.Though is all trying to explore countries in the world at present, effective substitute products are still few, and the exploitation of green feed additive uses task very urgent.

At present, some main antibiotic natural substitute for mainly contain 3 classes, i.e. probiotics, oligosaccharides and plant extracts, and wherein probiotic receives much concern.

So-called probiotic refers to the microorganism to live, after the animal absorption is a certain amount of, the intestines and stomach privileged site have the bacterial strain of some can adhesion, field planting and growth, thereby mainly by improving the balance of micro ecology of gastrointestinal tract, to growth of animal and the healthy preparation that plays beneficial effect.The main effect of probiotic is to improve the general level of the health of animal by improving GI microorganism species balance, and then improves the ability of animal opposing pathogen and promote growth of animal.

Comparatively extensive to the research of probiotic both at home and abroad, mainly concentrate in the exploitation of physiological action, the mechanism of action and probiotics preparation of the distribution situation of beneficial microbe in animal gastrointestinal tract, probio, and obtained certain technological achievement.Technology such as enteron aisle location, separation, evaluation, directive breeding and the cultivation of probio are all very ripe.Now finish the separation of multiple bacterium in the gastrointestinal tract of livestock and fowls and identified, and developed multiple probiotics preparation product.

Probiotic can be classified according to the bacterial classification kind, also can be divided into single culture probiotic and composite bacteria probiotic according to contained microorganism fungus kind quantity in the probiotic, the single culture probiotic mainly contains lactic acid bacteria class, bacillus category and yeast class etc., and the probiotic of composite bacteria then is the probiotic that is combined into by two or more bacterial classifications.

Probiotic action effect variation at present is very big, and main cause has: viable count is few in the product; The product specific aim is poor; Microorganism in the compound formulation is formed no clear and definite scientific basis etc.Therefore, we think, has only the composite prebiotics through strict composite design, just can carry more probiotic properties, could in intestines and stomach, have bigger deciding and grow the location potentiality, just can have the better application effect, therefore, the present invention is in national spark program " feeding super probiotic industrialization development (2010GA690108) ", Jiangsu Province scientific and technological enterprises innovation fund " feeding super probiotic production key technology research and industrialization development (BC2010142) ", Huai science and technology support plan countries such as " seed selection and the feed addictive research and development (SN0874) thereof of piglet benefit lactogenesis acidfast bacilli ", economize, under the support of city's project, through the years of researches exploitation, propose to be based upon the feeding super probiotic production method on different probiotic strains and the composite functional sugar specific function combination foundation.

Summary of the invention

The present invention proposes a kind of production method of feeding super probiotic product, carry out at spore bacillus that different benefits is sprouted, beneficial lactogenesis acidfast bacilli on the basis of directed functional screening, that selection has is antibiotic, secreting amylase, 3 genera lactobacillus of bile salt hydrolase activity and 1 series bacillus with secretion antibacterial peptide function, behind 3 grades of amplification fermentations, flocculating setting, obtain the thalline concentrated slurry respectively, the thalline concentrated slurry adds bag by the wall material and carries out spray-drying, obtains microcapsule bacterial powder separately; With shrimp shell or crab shell with the post processing of soda acid elder generation after, prepare shitosan, with the shitosan enzymolysis, by control enzyme concentration and hydrolysis time, obtain containing the mixed hydrolysis liquid of special ratios oligosaccharides, compound sugar, polysaccharide, hydrolyzate concentrates, obtain composite functional sugar after the drying; Microcapsule bacterial powder and composite functional sugar are mixed in specific proportions, obtain feeding super probiotic product.Different strains is brought into play distinctive prebiotic effect separately in the product, and the compound action by these bacterial strains, producing more benefit comes into force really, meanwhile, bacillus has " biology is taken oxygen by force " effect to lactic acid bacteria, consumes the oxygen of animal gastrointestinal tract, significantly promote the growth of Bacillus acidi lactici, good synergy is arranged between functional shitosan, chitosan oligosaccharide and the probio, can promote the propagation of probio, play the effect of " prebiotics ".

In the selection of producing bacterial strain, require selected bacterial classification to have specific function, that selection has is antibiotic, the Bacillus acidi lactici bacterial classification of secreting amylase, bile salt hydrolase activity each more than 1 strain, select to have more than bacillus 1 strain of prebiotic effect and secretion antibacterial peptide dual-use function; All bacterial strains all can tolerate the feed high-copper of hydrochloric acid in gastric juice level, 0.3% cholate and 250 mg/kg of pH 3.0, between each bacterial strain of tool favorable compatibility are arranged, not antagonism mutually.

The viable bacteria content proportion control of 3 genera lactobacillus and 4 kinds of probios of 1 series bacillus is 1:1:1:1 ~ 2 in the product, and total viable count is no less than 1010/g.To be controlled to be 100 ~ 500g/kg to composite functional sugar content in the product, and the chitosan oligosaccharide content that contains 2 ~ 6 monose is 50 ~ 100g/kg.

Description of drawings

Accompanying drawing is feeding super probiotic production technology route map.

The specific embodiment

The present invention will be further elaborated by following embodiment.

Embodiment:

On the basis of collecting a large amount of Bacillus acidi lacticis and Bacillus subtilis strain, by experiment sieving, obtain meeting Bacillus acidi lactici 9 strains of following feature, bacillus 3 strains:

1. acid resistance: handle 12 h through pH 3.0, viable count can significantly not reduce;

2. bile tolerance: in the environment that contains 0.3% cholate, can well grow;

3. anti-high-copper: in the nutrient broth that contains 250 mg/kg copper, can well grow;

4. stick ability: non-specific adhesive force (thalline hydrophobicity) is the surface hydrophobicity power to hexadecane (Hexadecane)〉85%; The specificity adhesive force is that the cell number of each pig ileal epithelium cell adhesion is above 15.

By the test to the bacterial strain biological function, filter out have antibiotic, secreting amylase respectively, each 1 strain of the Bacillus acidi lactici of bile salt hydrolase activity and bacillus subtilis 1 strain with secretion antibacterial peptide function, serve as to produce bacterial classification with it, behind 3 grades of amplification fermentations, flocculating setting, obtain the thalline concentrated slurry respectively, the thalline concentrated slurry adds beta-schardinger dextrin-as wrapping by the wall material and carrying out spray-drying, obtains microcapsule bacterial powder separately; 4 kinds of bacterium powder are evenly mixed in the ratio of 1:1:1:1, obtain composite micro-capsule bacterium powder.

Dry shrimp shell meal is soaked 24 h with 5% watery hydrochloric acid, 4 h are boiled in washing back in concentration is 5% NaOH weak solution, 1.5 h are handled in the washing back under 110 ℃ in 50% NaOH solution, washing and drying obtains shitosan, shitosan is mixed with the shitosan acetic acid solution of 0.03g/ mL with acetic acid solution, add cellulose hydrolyzation 4 h, the hydrolyzate vacuum concentrates, obtain composite functional sugar after the spray-drying.

Composite micro-capsule bacterium powder and composite functional sugar are mixed in the 2:1 ratio, obtain feeding super probiotic product.

In the full price mixed fodder, add 0.1%～0.2% feeding super probiotic product, can reach the result of use similar to antibiotic, feed conversion rate improves 6%～11% to chicken, pig is improved 7%～15%, can substitute the use of fodder antibiotics, produce " nonreactive " animal products of green safety.

Claims (4)

1. the production method of a feeding super probiotic product, carry out at spore bacillus that different benefits is sprouted, beneficial lactogenesis acidfast bacilli on the basis of directed functional screening, that selection has is antibiotic, secreting amylase, 3 genera lactobacillus of bile salt hydrolase activity and 1 series bacillus with secretion antibacterial peptide function, behind 3 grades of amplification fermentations, flocculating setting, obtain the thalline concentrated slurry respectively, the thalline concentrated slurry adds bag by the wall material and carries out spray-drying, obtains microcapsule bacterial powder separately; With shrimp shell or crab shell with the post processing of soda acid elder generation after, prepare shitosan, with the shitosan enzymolysis, by control enzyme concentration and hydrolysis time, obtain containing the mixed hydrolysis liquid of special ratios oligosaccharides, compound sugar, polysaccharide, hydrolyzate concentrates, obtain composite functional sugar after the drying; Microcapsule bacterial powder and composite functional sugar are mixed in specific proportions, obtain feeding super probiotic product.

2. method according to claim 1, it is characterized in that: in the selection of producing bacterial strain, selected bacterial classification has specific function, require to select to have antibiotic, secreting amylase, bile salt hydrolase activity the Bacillus acidi lactici bacterial classification each more than 1 strain, select to have more than bacillus 1 strain of prebiotic effect and secretion antibacterial peptide dual-use function; All bacterial strains all can tolerate the feed high-copper of hydrochloric acid in gastric juice level, 0.3% cholate and 250 mg/kg of pH 3.0, and between each bacterial strain favorable compatibility are arranged, not antagonism mutually.

3. method according to claim 1, it is characterized in that: the viable bacteria content ratio of 3 genera lactobacillus and 4 kinds of probios of 1 series bacillus is 1:1:1:1 ~ 2 in the feeding super probiotic product, and total viable count is no less than 10 ¹⁰Individual/g.

4. method according to claim 1 is characterized in that: composite functional sugar content is 100 ~ 500 g/kg in the feeding super probiotic product, and the chitosan oligosaccharide content that contains 2 ~ 6 monose is 50 ~ 100 g/kg.