CN103202286B - Electronic program controls at the uniform velocity to lower the temperature freezing plant - Google Patents
Electronic program controls at the uniform velocity to lower the temperature freezing plant Download PDFInfo
- Publication number
- CN103202286B CN103202286B CN201310124666.3A CN201310124666A CN103202286B CN 103202286 B CN103202286 B CN 103202286B CN 201310124666 A CN201310124666 A CN 201310124666A CN 103202286 B CN103202286 B CN 103202286B
- Authority
- CN
- China
- Prior art keywords
- temperature
- electronic program
- uniform velocity
- freezing
- lock chamber
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Abstract
The invention provides a kind of electronic program and control at the uniform velocity to lower the temperature freezing plant, this device has an at the uniform velocity cooling can putting into cryogenic freezing refrigerator-freezer to freeze cavity, its interior metal lead the lumen pore warm lock chamber evenly having and lays multiple cryopreservation tube, bosom has temperature sensor to be connected to be placed on the electronic program controller of cryogenic freezing refrigerator-freezer outside, lead warm lock chamber periphery and be closely surrounded by the metal temperature-control cover that a circle is equipped with micro-heating element heater, controlled by electronic program controller; The insulated enclosure upper cover that its top has loose-leaf connecting band to seal clamp ensures its inner sealing.This device can be different to different Cell and organism setting tissue freezing procedures, accurately control the speed that inner cryopreservation tube freezes, to ensure the Cell and organism intact tissue after freezing.Beneficial effect is: provide the freezing plant that a kind of intelligence accurately controls, and avoids Cell and organism sample and causes because freezing speed is unreasonable membranolysis and inside to analyse the phenomenons such as liquid crystallization, improve the frozen survival rate of Cell and organism sample.
Description
Technical field
The invention provides a kind of electronic program and control at the uniform velocity to lower the temperature freezing plant.
Background technology
In the lab, a lot of scientific research and experiment need experiment sample as cell, skin histology, biological specimens etc. freeze, prior art be the cryopreservation tube that experiment sample is housed directly is put into carry out in cryogenic freezing refrigerator-freezer frozen, or only add an isolating seal cover and reduce freezing speed, owing to needing different freezing temperatures and freezing speed when different Cell and organism organizes quick-frozen to preserve, and directly freezing speed that is frozen or that only add isolating seal cover is all out of contior, therefore cause common membranolysis and inside to analyse the bad phenomenon such as liquid crystallization because freezing speed is unreasonable, directly affects the result of experiment, experiment needs can not be met, for various scientific research causes inconvenience.Existing electronic program cooling instrument adopts liquid nitrogen cooling method to need to consume a large amount of liquid nitrogen and price is high, large etc. the disadvantages affect application of operation inconvenience, floor space in the market, therefore developing a kind of not only simple and efficiently does not need to use liquid nitrogen but also can the freezing plant of accurately control freezing temperature and freezing speed, improve the frozen survival rate of Cell and organism sample in scientific research and experiment, become current urgent needs.
Summary of the invention
For solving the problem, the object of this invention is to provide a kind of electronic program and control at the uniform velocity to lower the temperature freezing plant, difference when package unit can be preserved according to different cell, skin histology and biological tissue's quick-frozen requires to set freezing procedures, and accurately control the speed that Internal biological cryopreservation tube freezes, to ensure the Cell and organism intact tissue after freezing according to the cooling rate that program setting is good.
For achieving the above object, technical scheme of the present invention is to provide a kind of electronic program and controls at the uniform velocity to lower the temperature freezing plant, this device includes the at the uniform velocity cooling can putting into the storage of cryogenic freezing refrigerator-freezer and freezes cavity, its inside be with metal make lead warm lock chamber, evenly have the lumen pore laying multiple cryopreservation tube above, the position, bosom of cavity is provided with temperature sensor, be connected to by lead-in wire on the electronic program controller being placed in cryogenic freezing refrigerator-freezer outside, lead and to be closely surrounded by a circle outside warm lock chamber the metal temperature-control cover that micro-heating element heater heats by Electric heating is installed, metal temperature-control cover is also connected to electronic program controller by lead-in wire, at the uniform velocity cooling freezes cavity top by gemel connection to the insulated enclosure upper cover having sealing clamp, ensure that at the uniform velocity the inner sealing of cavity is freezed in cooling, difference when quick-frozen can be organized to preserve according to different Cell and organism requires to set freezing procedures, and accurately control according to the cooling rate that program setting is good the speed that Internal biological cryopreservation tube freezes, to ensure the Cell and organism intact tissue after freezing.
Its beneficial effect is: provide the freezing plant that a kind of intelligence accurately controls, thus greatly reduce Cell and organism sample in freezing process, cause because freezing speed is unreasonable common membranolysis and inside to analyse the bad phenomenon such as liquid crystallization, improve the frozen survival rate of Cell and organism sample in scientific research and experiment, fill up this respect blank.
Accompanying drawing explanation
Fig. 1 is that electronic program of the present invention controls at the uniform velocity to lower the temperature the structural representation of freezing plant.
In figure:
1, clamp 2, insulated enclosure upper cover 3, silica gel ring 4, hinge is sealed
5, lead-in wire 6, electronic program controller 7, temperature comparison module 8, program writing module
9, temperature control modules 10, micro-heating element heater 11, temperature sensor 12, cavity
13, metal temperature-control cover 14, lumen pore 15, lead warm lock chamber.
Embodiment
By reference to the accompanying drawings and embodiment the present invention is illustrated.
As shown in Figure 1, the invention provides a kind of electronic program and control at the uniform velocity to lower the temperature freezing plant, this device includes the at the uniform velocity cooling can putting into the storage of cryogenic freezing refrigerator-freezer and freezes cavity 12, its inside be with metal make lead warm lock chamber 15, evenly have the lumen pore 14 laying multiple cryopreservation tube above, the position, bosom of cavity is provided with temperature sensor 11, by go between 5 be connected to be placed in cryogenic freezing refrigerator-freezer outside electronic program controller 6 on, lead closely to be surrounded by outside warm lock chamber 15 that a circle is provided with can the metal temperature-control cover 13 that heats by Electric heating of micro-heating element heater, metal temperature-control cover 13 is also connected to electronic program controller 6 by lead-in wire 5, at the uniform velocity cavity 12 top is connected to sealing clamp 1 insulated enclosure upper cover 2 by hinge 4 is freezed in cooling, ensure that at the uniform velocity the inner sealing of cavity 12 is freezed in cooling, leading warm lock chamber 15 is be made up of the corrosion-resistant metal materials of temperature good conductor, in cylindrical shape, a temperature sensors of high precision 11 is equipped with at its center, around the lumen pore 14 that temperature sensor 11 is the conical or circle hole shape compositions in a circle lower end, will the combine closely all kinds of cryopreservation tube appearance and size of laying and size of its size conforms is beneficial to fully conduct refrigeration, metal temperature-control cover 13 is that of being made up of the corrosion-resistant metal materials of temperature good conductor is enclosed in the cylinder of leading warm lock chamber 15 periphery closely, the inner barrel of cylinder is evenly arranged micro-heating element heater 10, controls to drive micro-heating element heater (10) to form trace to the cylinder of metal temperature-control cover (13) entirety electrified regulation mode to heat to control to lead the cryopreservation tube chilling rate that warm lock chamber (15) lumen orifice (14) manages down at electronic program controller (6), insulated enclosure upper cover 2 is domes that the insulator of a bad heat conduction is made, and fixes at the uniform velocity cooling freeze cavity 12 and sealing upper cover 2 by hinge 4 and sealing clamp 1, and sealing cover rim is equipped with silica gel ring 3 that can be low temperature resistant and carrys out the cryopreservation tube of sealed inside, electronic program controller 6 is made up of the different circuit modules be arranged in an electrical appliance kit, and program writing module 8 is connected on the supervisory keyboard of electrical appliance kit upper surface, temperature comparison module 7 is from the signal of telecommunication sense temperature decrease speed being arranged on temperature sensor 11 transmission led warm lock chamber 15, be presented on screen, be delivered to temperature control modules 9 simultaneously, temperature control modules 9 controls the micro-heating element heater 10 on metal temperature-control cover 13 according to the signal of temperature comparison module 7 again, different extent control of heating leads Internal biological cryopreservation tube cooling freezing speed on warm lock chamber 15, to ensure the Cell and organism intact tissue after freezing.
Embodiment
A kind of electronic program of the present invention is used to control at the uniform velocity to lower the temperature before freezing plant and should first get out experiment sample and cryopreservation tube, experiment sample is installed to good seal in cryopreservation tube, open at the uniform velocity cooling and freeze the sealing clamp 1 of the insulated enclosure upper cover 2 above cavity 12, raise insulated enclosure upper cover 2, the cryopreservation tube installing experiment sample being put into successively at the uniform velocity cooling freezes inside the lumen pore 14 of leading warm lock chamber 15 li of cavity 12 li, then build insulated enclosure upper cover 2, lock sealing clamp 1 keeps cavity 12 to seal, the cavity of good seal is put into cryogenic freezing refrigerator-freezer to store, electronic program controller 6 is placed on the outside of cryogenic freezing refrigerator-freezer, open the power supply of electronic program controller 6, by the supervisory keyboard of electronic program controller upper surface input freeze the freezing procedures of experiment sample, laboratory sample starts to freeze, from the signal of telecommunication sense temperature decrease speed being arranged on temperature sensor 11 transmission led warm lock chamber 15, be presented on electronic program controller 6 screen, be delivered to temperature control modules 9 simultaneously, temperature control modules 9 controls the micro-heating element heater 10 on metal temperature-control cover 13 according to the signal of temperature comparison module 7 again, Internal biological cryopreservation tube cooling freezing speed is led on warm lock chamber 15 by Electric heating different extent control of heating, to ensure the Cell and organism intact tissue after freezing, freeze the power supply of rear closedown electronic program controller 6, from cryogenic freezing refrigerator-freezer, taken out cavity 12, opened sealing clamp 1, start insulated enclosure upper cover 2, taken out cryopreservation tube and carry out the freezing of routine.
Claims (5)
1. an electronic program controls at the uniform velocity to lower the temperature freezing plant, it is characterized in that: this device includes the at the uniform velocity cooling can putting into the storage of cryogenic freezing refrigerator-freezer and freezes cavity (12), its inside be with metal make lead warm lock chamber (15), evenly have the lumen pore (14) laying multiple cryopreservation tube above, the position, bosom of cavity is provided with temperature sensor (11), be connected to by lead-in wire (5) on the electronic program controller (6) being placed in cryogenic freezing refrigerator-freezer outside, electronic program controller (6) is by the program writing module (8) be arranged in an electrical appliance kit, temperature comparison module (7) and temperature control modules (9) composition, electronic program controller (6) is connected to metal temperature-control cover (13) by lead-in wire (5), lead warm lock chamber (15) outside and be closely surrounded by a circle metal temperature-control cover (13), metal temperature-control cover (13) is the cylinder be made up of the corrosion-resistant metal materials of temperature good conductor, the inner barrel of metal temperature-control cover (13) is provided with micro-heating element heater (10), under electronic program controller (6) controls, drive micro-heating element heater (10) to form trace to the overall electrified regulation mode of the cylinder of metal temperature-control cover (13) heat to control to lead the inner cryopreservation tube chilling rate of warm lock chamber (15) lumen orifice (14), at the uniform velocity cavity (12) top is connected to sealing clamp (1) insulated enclosure upper cover (2) by hinge (4) is freezed in cooling, ensure that at the uniform velocity the inner sealing of cavity (12) is freezed in cooling.
2. electronic program according to claim 1 controls at the uniform velocity to lower the temperature freezing plant, it is characterized in that: described in lead warm lock chamber (15) be made up of the corrosion-resistant metal materials of temperature good conductor, in cylindrical shape, a temperature sensors of high precision (11) is equipped with at its center, around the lumen pore (14) that temperature sensor (11) is the conical or circle hole shape composition in a circle lower end, will the combine closely all kinds of cryopreservation tube appearance and size of laying and size of its size conforms is beneficial to fully conduct refrigeration.
3. electronic program according to claim 1 controls at the uniform velocity to lower the temperature freezing plant, it is characterized in that: described metal temperature-control cover (13) is that of being made up of the corrosion-resistant metal materials of temperature good conductor is closely enclosed in and leads the peripheral cylinder of warm lock chamber (15), the inner barrel of cylinder is evenly arranged micro-heating element heater (10), under electronic program controller (6) controls, drive micro-heating element heater (10) to form trace to the overall electrified regulation mode of the cylinder of metal temperature-control cover (13) heat to control to lead the inner cryopreservation tube chilling rate of warm lock chamber (15) lumen orifice (14).
4. electronic program according to claim 1 controls at the uniform velocity to lower the temperature freezing plant, it is characterized in that: described insulated enclosure upper cover (2) is the dome that the insulator of a bad heat conduction is made, fix at the uniform velocity cooling by hinge (4) and sealing clamp (1) and freeze cavity (12) and sealing upper cover (2), seal cover rim be equipped with can be low temperature resistant silica gel ring (3) carry out the cryopreservation tube of sealed inside.
5. electronic program according to claim 1 controls at the uniform velocity to lower the temperature freezing plant, it is characterized in that: described electronic program controller (6) is made up of the different circuit modules be arranged in an electrical appliance kit, program writing module (8) is connected on the supervisory keyboard of electrical appliance kit upper surface; Temperature comparison module (7) is from the signal of telecommunication sense temperature decrease speed being arranged on the temperature sensor (11) of leading warm lock chamber (15) and transmitting, be presented on screen, be delivered to temperature control modules (9) simultaneously, temperature control modules (9) controls the micro-heating element heater (10) in metal temperature-control cover (13) again according to the signal of temperature comparison module (7), different extent control of heating leads warm lock chamber (15) upper cryopreservation tube cooling freezing speed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310124666.3A CN103202286B (en) | 2013-04-11 | 2013-04-11 | Electronic program controls at the uniform velocity to lower the temperature freezing plant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310124666.3A CN103202286B (en) | 2013-04-11 | 2013-04-11 | Electronic program controls at the uniform velocity to lower the temperature freezing plant |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103202286A CN103202286A (en) | 2013-07-17 |
| CN103202286B true CN103202286B (en) | 2015-09-02 |
Family
ID=48749874
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310124666.3A Expired - Fee Related CN103202286B (en) | 2013-04-11 | 2013-04-11 | Electronic program controls at the uniform velocity to lower the temperature freezing plant |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103202286B (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103704199B (en) * | 2013-09-30 | 2015-07-22 | 杭州柏恒科技有限公司 | Cell freezing box |
| CN103548815B (en) * | 2013-11-18 | 2015-06-10 | 王意 | Programmed cell freezing box with direct-in liquid nitrogen |
| CN107318827B (en) * | 2017-07-03 | 2020-12-01 | 上海理工大学 | Medical speed-regulating and cooling device with communication function |
| CN110192551A (en) * | 2018-12-28 | 2019-09-03 | 广东省心血管病研究所 | Cooling system and control method used in a kind of stem cell cryopreserving process |
| CN109580703A (en) * | 2019-01-25 | 2019-04-05 | 石家庄铁道大学 | Soil body freezing point measuring system and soil body freezing point measurement method |
| CN114739075B (en) * | 2022-03-31 | 2023-06-16 | 青岛海尔生物医疗股份有限公司 | Method and device for cooling program cooling instrument and program cooling instrument |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1367380A (en) * | 2001-01-21 | 2002-09-04 | 中国科学院理化技术研究所 | Detection method for detecting frozen and stored biological sample quality and its equipment |
| CN101085979A (en) * | 2007-06-26 | 2007-12-12 | 上海交通大学医学院附属瑞金医院 | Cell freezing apparatus |
| CN101158520A (en) * | 2007-11-13 | 2008-04-09 | 上海理工大学 | Biological material low temperature storage device |
| CN101849535A (en) * | 2010-05-24 | 2010-10-06 | 中国水产科学研究院黄海水产研究所 | Portable fish sperm frozen cooling device |
| WO2010129817A1 (en) * | 2009-05-07 | 2010-11-11 | Tissue Genesis, Inc. | Adipose tissue collection and pre-processing devices for use in liposuction procedure |
| CN201752216U (en) * | 2010-06-04 | 2011-03-02 | 北京慧荣和科技有限公司 | Program temperature reduction box |
| CN202404466U (en) * | 2011-12-30 | 2012-08-29 | 潍坊阳光传媒科技职业培训学校 | Electronic automatic temperature and pressure control device for reaction container |
-
2013
- 2013-04-11 CN CN201310124666.3A patent/CN103202286B/en not_active Expired - Fee Related
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1367380A (en) * | 2001-01-21 | 2002-09-04 | 中国科学院理化技术研究所 | Detection method for detecting frozen and stored biological sample quality and its equipment |
| CN101085979A (en) * | 2007-06-26 | 2007-12-12 | 上海交通大学医学院附属瑞金医院 | Cell freezing apparatus |
| CN101158520A (en) * | 2007-11-13 | 2008-04-09 | 上海理工大学 | Biological material low temperature storage device |
| WO2010129817A1 (en) * | 2009-05-07 | 2010-11-11 | Tissue Genesis, Inc. | Adipose tissue collection and pre-processing devices for use in liposuction procedure |
| CN101849535A (en) * | 2010-05-24 | 2010-10-06 | 中国水产科学研究院黄海水产研究所 | Portable fish sperm frozen cooling device |
| CN201752216U (en) * | 2010-06-04 | 2011-03-02 | 北京慧荣和科技有限公司 | Program temperature reduction box |
| CN202404466U (en) * | 2011-12-30 | 2012-08-29 | 潍坊阳光传媒科技职业培训学校 | Electronic automatic temperature and pressure control device for reaction container |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103202286A (en) | 2013-07-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103202286B (en) | Electronic program controls at the uniform velocity to lower the temperature freezing plant | |
| CN103941778B (en) | The temperature control system of micro constant-temperature case and method | |
| CN103677011B (en) | One is applicable to extend blackbody wide temperature range control system under vacuum condition | |
| CN207258375U (en) | A kind of short distance blood collection low temperature carrying case | |
| CN203181880U (en) | Constant-speed temperature-reducing and freezing device controlled by electronic program | |
| CN207221947U (en) | A kind of segmented micro-fluidic chip temperature regulating device | |
| CN109628297A (en) | A kind of microfluidic high-throughput biological sample drop jelly save set | |
| CN201136864Y (en) | Programmed cell freezing instrument with quick temperature reduction and temperature recovery functions | |
| CN204232157U (en) | A kind of use for laboratory cryopreservation tube freezing plant | |
| CN201464703U (en) | Cryomicroscope object stage | |
| CN210270643U (en) | Liquid nitrogen cooling automatic controller | |
| CN104299482A (en) | Bi-direction adjustable semiconductor temperature control teaching experiment system | |
| CN201729836U (en) | Cell culture dish convenient for perfusion and heat insulation | |
| RU2305233C1 (en) | Portable thermostat for biological investigations | |
| CN201097226Y (en) | A mini-temperature adjusting device | |
| CN203812113U (en) | Metal bath temperature control system | |
| CN209555392U (en) | Constant temperature electrophoresis control device | |
| CN203269947U (en) | Miniature carbon dioxide cell incubator with fluid pipe interface and electric interface | |
| CN208175928U (en) | A kind of cell save set | |
| CN203551507U (en) | Simple temperature control PCR-SSCP gel electrophoresis device | |
| CN206037543U (en) | Medicament easily transports save set with constant temperature | |
| CN205342134U (en) | Superconductive cable vacuum soldering system | |
| CN203264385U (en) | Funnel with functions of temperature display and temperature regulation | |
| CN220132221U (en) | Culture dish for microbiological measurement and analysis | |
| CN217628420U (en) | Biological safety cabinet and multifunctional constant temperature platform thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150902 Termination date: 20170411 |