CN217628420U - Biological safety cabinet and multifunctional constant temperature platform thereof - Google Patents
Biological safety cabinet and multifunctional constant temperature platform thereof Download PDFInfo
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- CN217628420U CN217628420U CN202220925011.0U CN202220925011U CN217628420U CN 217628420 U CN217628420 U CN 217628420U CN 202220925011 U CN202220925011 U CN 202220925011U CN 217628420 U CN217628420 U CN 217628420U
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Abstract
The application discloses a biological safety cabinet and a multifunctional constant temperature table thereof, wherein the multifunctional constant temperature table comprises a constant temperature layer frame, a constant temperature box, a heating module, a refrigerating module and a temperature control module, and a clamping groove for placing a culture dish is arranged on the constant temperature layer frame; the constant temperature box is connected with the constant temperature layer frame, and a slotted hole for placing the pipe body is formed in the constant temperature box; the heating module is arranged on the constant-temperature layer frame and used for heating the constant-temperature layer frame; the refrigeration module is arranged in the constant temperature box and is used for refrigerating the constant temperature box; the temperature control module is arranged in the constant temperature box and connected with the heating module and the refrigerating module, and the temperature control module is used for controlling the heating module and the refrigerating module to work. Above-mentioned multi-functional constant temperature platform has the dual function of cell digestion and cell cryopreserving, during the operation, directly places multi-functional constant temperature platform in the biological safety cabinet, reduces and removes, avoids polluting, simplifies the operation flow to reduce personnel's operation degree of difficulty and work load, meanwhile, need not the transfer, reduce innocent cell pollution risk.
Description
Technical Field
The application relates to the technical field of cell culture, in particular to a multifunctional constant-temperature table. The application also relates to a biological safety cabinet with the multifunctional constant temperature table.
Background
It is known that cells are continuously proliferated during in vitro culture and easily grow in a multilayer aggregate, and single cells are often needed in subsequent experiments, and proper pancreatin is usually added into a culture dish to digest the cells into single cells, wherein the digestion condition of the pancreatin is 37 ℃.
The scheme of the prior art is as follows: after the cell confluence reaches 80-90%, the cells can be passaged (cell passage: after the cells grow into a dense monolayer in a flask/dish, the cells are substantially saturated and must be passaged (re-cultured) in order to continue to grow and expand the number of cells). Adding a proper amount of pancreatin into a plurality of cell culture dishes which are washed by saline water or PBS, timing after adding the pancreatin, taking out the culture dishes from the biological safety cabinet, transferring the culture dishes into a carbon dioxide incubator at 37 ℃ for digestion, taking out the culture dishes from the incubator after digesting for a certain time, transferring the culture dishes into the biological safety cabinet for the next operation by an operator, however, if the number of the dishes is large during transferring the culture dishes, the cover opening and the dropping of the culture dishes can be caused by a slight improper transferring process. In addition, when the cells are frozen, the cells are stored at low temperature by a freezing ice box in the traditional mode, but the freezing ice box needs to be placed in a refrigerator at the temperature of-20 ℃ in advance and then transferred into a biological safety cabinet for cell freezing. Therefore, in order to meet the operation requirements of cell digestion and cryopreservation, a person skilled in the art needs to provide a multifunctional thermostatic table which is multifunctional and can perform cell digestion and cryopreservation operations in a biosafety cabinet.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a multi-functional constant temperature platform can carry out cell digestion and cryopreserving operation in a biosafety cabinet to can reduce and remove, avoid polluting. Another object of the present application is to provide a biosafety cabinet comprising the above multifunctional thermostatic station.
In order to achieve the above objects, the present application provides a multifunctional thermostatic table, which is used to be built in a biosafety cabinet, comprising:
the constant-temperature shelf is provided with a clamping groove for placing a culture dish;
the constant temperature box is connected with the constant temperature layer frame, and a slotted hole for placing the pipe body is formed in the constant temperature box;
the heating module is arranged on the constant-temperature layer frame and used for heating the constant-temperature layer frame;
the refrigerating module is arranged in the constant temperature box and is used for refrigerating the constant temperature box;
and the temperature control module is arranged in the constant temperature box, is connected with the heating module and the refrigerating module and is used for controlling the heating module and the refrigerating module to work.
In some embodiments, the constant temperature shelf comprises at least two layers of platens, and two rows of the clamping grooves are arranged on any one of the platens, and the two rows of the clamping grooves are different in size.
In some embodiments, at least two layers of the platens are arranged in parallel and equally spaced apart.
In some embodiments, said incubator is provided with:
a plurality of rows of first slots for placing freezing tubes;
a plurality of rows of second slotted holes for placing centrifuge tubes;
wherein the diameters of the rows of first slotted holes are smaller than the diameters of the rows of second slotted holes.
In some embodiments, the incubator further comprises a pressure detection assembly, the pressure detection assembly is arranged on the constant-temperature shelf, and the pressure detection assembly is used for detecting whether a culture dish is placed on the constant-temperature shelf.
In some embodiments, the incubator further comprises a time and temperature display control module, the time and temperature display control module is arranged on the incubator and connected with the pressure detection assembly and the temperature control module, and the time and temperature display control module is used for timing after placing a culture dish on the constant temperature shelf and displaying the current temperature of the constant temperature shelf.
In some embodiments, a prompt module is arranged on the incubator, the prompt module is connected with the time and temperature display control module, and the prompt module gives an alarm prompt after the time and temperature display control module finishes timing so as to remind an operator to take away the culture dish.
In some embodiments, the thermostatic shelf and the incubator are a unitary structure.
In some embodiments, a power interface is provided on the incubator, and the power interface is used for connecting a power supply.
The application also provides a biological safety cabinet, which comprises a cabinet body and a multifunctional constant temperature table arranged in the cabinet body.
Compared with the background art, the multifunctional constant temperature table provided by the embodiment of the application is used for being arranged in a biological safety cabinet, and comprises a constant temperature layer frame, a constant temperature box, a heating module, a refrigerating module and a temperature control module, wherein the constant temperature layer frame is provided with a clamping groove for placing a culture dish; the constant temperature box is connected with the constant temperature layer frame, and a slotted hole for placing the pipe body is formed in the constant temperature box; the heating module is arranged on the constant-temperature layer frame and used for heating the constant-temperature layer frame; the refrigeration module is arranged in the constant temperature box and is used for refrigerating the constant temperature box; the temperature control module is arranged in the constant temperature box and connected with the heating module and the refrigerating module, and the temperature control module is used for controlling the heating module and the refrigerating module to work. Specifically, when cells are digested, the culture dish is placed in a clamping groove on a constant-temperature shelf and fixed, and the heating module is controlled by the temperature control module to heat the constant-temperature shelf so as to meet the temperature requirement of cell digestion; when carrying out cell cryopreserving, place the body in the slotted hole on the thermostated container fixedly to refrigerate the thermostated container through temperature control module control refrigeration module, in order to satisfy the temperature demand that the cell cryopreserved. Compare in traditional constant temperature platform, the multi-functional constant temperature platform that this application embodiment provided has the dual function of cell digestion and cell cryopreserving, during the operation, directly arranges multi-functional constant temperature platform in the biosafety cabinet, reduces and removes, avoids polluting, simplifies the operation flow to reduce personnel's operation degree of difficulty and work load, meanwhile, need not to shift, avoid uncapping, fall the lid risk, reduce innocent cell pollution risk, make it keep steady state.
Drawings
In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly introduced below, it is obvious that the drawings in the following description are only embodiments of the present application, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.
Fig. 1 is a schematic structural diagram of a multifunctional thermostatic table in an embodiment of the present application.
Wherein:
1-a thermostat, 2-a time and temperature display control module, 3-a power interface, 4-a first slot, 5-a second slot, 6-a constant temperature shelf and 7-a card slot.
Detailed Description
The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all of the embodiments. All other embodiments obtained by a person of ordinary skill in the art based on the embodiments in the present application without making any creative effort belong to the protection scope of the present application.
The core of this application is to provide a multi-functional constant temperature platform, can carry out cell digestion and cryopreserving operation in a biosafety cabinet to can reduce and remove, avoid polluting. Another core of the present application is to provide a biosafety cabinet comprising the multifunctional thermostatic station.
In order to enable those skilled in the art to better understand the scheme of the present application, the present application will be described in further detail with reference to the accompanying drawings and the detailed description.
It should be noted that the following directional terms such as "upper end, lower end, left side, right side" and the like are defined based on the drawings of the specification.
Referring to fig. 1, fig. 1 is a schematic structural diagram of a multifunctional thermostatic table in an embodiment of the present application.
The multifunctional constant temperature table provided by the embodiment of the application is used for being arranged in a biological safety cabinet, and comprises a constant temperature layer frame 6, a constant temperature box 1, a heating module, a refrigerating module and a temperature control module, wherein a clamping groove 7 for placing a culture dish is arranged on the constant temperature layer frame 6; the constant temperature box 1 is connected with a constant temperature layer frame 6, and a slotted hole for placing a pipe body is formed in the constant temperature box 1; the heating module is arranged on the constant-temperature layer frame 6 and is used for heating the constant-temperature layer frame 6; the refrigeration module is arranged in the thermostat 1 and is used for refrigerating the thermostat 1; the temperature control module is arranged in the constant temperature box 1 and connected with the heating module and the refrigerating module, and the temperature control module is used for controlling the heating module and the refrigerating module to work.
Specifically, when cells are digested, the culture dish is placed in the clamping groove 7 on the constant-temperature shelf 6 for fixation, and the heating module is controlled by the temperature control module to heat the constant-temperature shelf 6 so as to meet the temperature requirement of cell digestion; when carrying out cell cryopreserving, place the body in the slotted hole on thermostated container 1 fixed to refrigerate thermostated container 1 through temperature control module control refrigeration module, in order to satisfy the temperature demand that cell cryopreserved.
Compare in traditional constant temperature platform, the multi-functional constant temperature platform that this application embodiment provided has the dual function of cell digestion and cell cryopreserving, during the operation, directly arranges multi-functional constant temperature platform in biosafety cabinet, reduces and removes, avoids polluting, and the simplified operation flow to reduce personnel's operation degree of difficulty and work load, meanwhile, need not to shift, avoid uncapping, fall and cover the risk, reduce innocent cell pollution risk, make it keep steady state.
It should be noted that the heating function of the heating module, the cooling function of the cooling module, and the temperature control function of the temperature control module are all well known by those skilled in the art, and are not important for protection in the present application, and the important point in the present application is to realize the temperature control function for the constant temperature layer rack 6 and the incubator 1 through the connection relationship of the temperature control module, the heating module, and the cooling module.
Of course, according to actual needs, the heating module may be a heating wire, and the refrigeration module may be a condenser tube, a compressor, or other components. The temperature control module may be a PLC.
In some embodiments, the constant temperature shelf 6 comprises at least two layers of platens, and two rows of slots 7 are provided on any one platen, the two rows of slots 7 being different in size.
Specifically, the thickness of any table board is 2cm, the diameter of the first row of clamping grooves 7 is 10cm, the depth of the first row of clamping grooves 7 is 0.5cm, and the diameter of the second row of clamping grooves 7 is 6cm, and the depth of the second row of clamping grooves 7 is 0.5cm.
In some embodiments, at least two layers of platens are arranged in parallel and equally spaced apart. For example, the constant temperature shelf 6 includes four layers of platens, and the spacing between any two adjacent layers of platens is the same.
In some embodiments, the incubator 1 is provided with a plurality of rows of first slots 4 and a plurality of rows of second slots 5, wherein the first slots 4 are used for placing the cryopreservation tubes; the second slotted hole 5 is used for placing a centrifugal tube; wherein the diameter of the plurality of rows of first slots 4 is smaller than the diameter of the plurality of rows of second slots 5.
Specifically, the diameter of any first slot 4 is 11mm, the depth is 20mm, and 20 freezing tubes/EP tubes of 2ml can be placed in a plurality of rows of first slots 4; the second slotted hole 5 comprises two slotted holes, wherein one slotted hole is 15mm in diameter and 85mm in depth, the number of the slotted holes is 8, and a 15ml centrifugal tube can be placed in the slotted hole, the other slotted hole is 28mm in diameter and 100mm in depth, the number of the slotted holes is 4, and a 50ml centrifugal tube can be placed in the slotted hole.
In some embodiments, the multifunctional thermostatic table further comprises a pressure detection component, the pressure detection component is arranged on the thermostatic layer frame 6, and the pressure detection component is used for detecting whether a culture dish is placed on the thermostatic layer frame 6. The pressure detection assembly is specifically a gravity sensor, the number of the gravity sensors is the same as that of the clamping grooves 7, and the gravity sensors are arranged at the bottom of the clamping grooves 7 in a one-to-one correspondence mode.
In some embodiments, the multifunctional thermostatic table further comprises a time and temperature display control module 2, the time and temperature display control module 2 is disposed on the incubator 1, the time and temperature display control module 2 is connected to the pressure detection assembly and the temperature control module, and the time and temperature display control module 2 is configured to time after placing the culture dish on the thermostatic shelf 6 and is configured to display the current temperature of the thermostatic shelf 6.
That is to say, the time and temperature display control module 2 can not only display the current temperature of the constant temperature shelf 6, but also after the cell culture dish is placed in the clamping groove 7, the gravity sensor in the bedplate senses the gravity of the dish and transmits a signal into the time and temperature display control module 2 to start timing, so that the timing function during cell digestion is met.
In addition, the real-time temperature can be visually displayed, and the time and temperature display control module 2 can also set the temperature and the heating time of the constant temperature table. The time and temperature display control module 2 can adopt a control panel, and the arrangement of the control panel can refer to the content of the prior art.
In some embodiments, the incubator 1 is provided with a prompting module, the prompting module is connected with the time and temperature display control module 2, and the prompting module sends out an alarm prompt after the time and temperature display control module 2 completes timing so as to remind an operator to take away the culture dish. The prompting module can be specifically an indicator light.
In some embodiments, thermostatic shelf 6 is a unitary structure with incubator 1. For example, the constant temperature layer frame 6 and the thermostat 1 are both made of heat conducting metal, and the constant temperature layer frame 6 and the thermostat 1 are integrally formed by the heat conducting metal.
In order to facilitate power-on, the thermostat 1 is provided with a power interface 3, and the power interface 3 is used for connecting a power supply.
The following describes the embodiments of cell digestion and cell cryopreservation.
Cell digestion example:
1. after the cultured cells reach 80% -90%, the operator takes out the cells to be passaged from the carbon dioxide incubator and transfers the cells to a biological safety cabinet for the passaging digestion operation.
2. And (3) opening the multifunctional constant temperature table which is positioned in the biological safety cabinet and is connected with the power supply, setting the temperature to be 37 ℃ through the time and temperature display control module 2, and placing the multifunctional constant temperature table for induction for 5-8 minutes (suitable for mesenchymal stem cells).
3. The supernatant was collected from the dish, the residual medium was washed off with PBS/saline, and appropriate amount of pancreatin was added.
4. Directly placing the culture dish of phi 10cm into which the pancreatin is added into the clamping groove 7 of the constant-temperature shelf 6, and automatically starting timing after the culture dish is placed.
After 5.5-8 minutes, the digestion is finished, the time and temperature display control module 2 controls the prompt module to give an alarm for prompting, and an operator takes the vessel down from the constant-temperature shelf 6 to stop the digestion and carry out the next operation.
Cell cryopreservation example:
1. when the cells are frozen, the temperature of the incubator 1 is set to 4 ℃ in advance, and the time is set to ∞ (constant temperature is maintained until the power is turned off).
2. When the operator centrifugates the cells, the frozen stock solution is prepared and placed in the second slot hole 5 for precooling.
3. After cell centrifugation is complete, the operator discards the culture medium supernatant and resuspends the cell pellet with the pre-cooled frozen stock solution.
4. After uniform mixing, the suspension is subpackaged into 2ml cryopreservation tubes according to the standard of 1.5ml per tube, the cryopreservation tube covers are screwed down and then placed into the pre-cooled first slotted holes 4, and the cryopreservation of the cells of the next tube can be continued.
After all cells are packed and cryopreserved, the cryopreserved tubes can be uniformly transferred to a program-controlled cooling box and placed in a refrigerator at the temperature of minus 80 ℃.
In conclusion:
firstly, in the traditional digestion process, after an operator adds a certain amount of pancreatin into a culture dish, because the digestion condition of the pancreatin is 37 ℃, the culture dish added with the pancreatin needs to be stacked and taken out from a biological safety cabinet and transferred to a carbon dioxide incubator. And the cells need a sterile culture environment, the cleanness grade in the biological safety cabinet is highest, and the pollution risk can be increased when the culture dish frequently enters and exits the safety cabinet. The embodiment of the application directly arranges the multifunctional constant-temperature table in the biological safety cabinet, reduces movement, avoids pollution and simplifies operation flow.
Secondly, if the number of the dishes is large when the culture dishes are transferred in the traditional culture dish transferring method, the dishes are not tightly clamped by the clamping grooves 7 when being stacked, and the transferring process needs to be very careful. According to the embodiment of the application, the cover opening and the dropping of the culture dish in the transfer process can be avoided.
Thirdly, the digestion time of different types of cells is indefinite, and an operator needs to count time by means of a timer to judge the digestion state of the cells, so that after the operator adds pancreatin, the operator needs to press the time outside the biological safety cabinet with assistance; meanwhile, when the culture dish is transferred traditionally, an operator needs to open and close the double-layer door of the carbon dioxide culture box, spray alcohol and the like by one hand, and the operation difficulty is high. This application embodiment is equipped with timing function, and need not to shift the culture dish, reducible labour and work load.
Fourthly, the frequent opening and closing of the incubator door is required during the traditional cell digestion, which can cause the carbon dioxide content and the temperature fluctuation in the incubator, is not favorable for the cells being cultured, and also increases the possibility of the pollution of the cells being cultured. The embodiment of the application can reduce the pollution risk of innocent cells and keep the innocent cells in a stable state.
Fifthly, when cells are frozen, the cells can be stored at low temperature by means of a freezing ice box in the traditional mode, but the freezing ice box needs to be placed in a refrigerator at the temperature of-20 ℃ in advance, and the cells need to be wiped by alcohol when being transferred into a biological safety cabinet for cell freezing, so that the operation is complex. And if the number of the freezing storage tubes is large, the temperature of the freezing storage box is increased, and the constant temperature cannot be kept. The embodiment of the application can set the temperature to be constant at 4 ℃, so that the operation can be simplified, the pollution can be avoided, and the situation that residual alcohol on the freezing ice box removes marks of the freezing pen can be avoided.
Sixthly, to the liquid that needs rewarming unfreezing, need place in the water bath usually, the water bath pollution risk is big, and the pollution emergence can be avoided in the operation of the no water source of this application embodiment.
The biological safety cabinet provided by the application comprises the multifunctional constant temperature table described in the specific embodiment; other parts of the biosafety cabinet can be referred to the prior art and are not expanded.
It is noted that, in this specification, relational terms such as first and second, and the like are used solely to distinguish one entity from another entity without necessarily requiring or implying any actual such relationship or order between such entities.
The biosafety cabinet and the multifunctional constant temperature table thereof provided by the application are described in detail above. The principle and the implementation of the present application are explained herein by using specific examples, and the above descriptions of the examples are only used to help understand the scheme and the core idea of the present application. It should be noted that, for those skilled in the art, it is possible to make several improvements and modifications to the present application without departing from the principle of the present application, and such improvements and modifications also fall within the scope of the claims of the present application.
Claims (10)
1. A multifunctional constant temperature table is arranged in a biological safety cabinet and is characterized by comprising:
the constant-temperature shelf is provided with a clamping groove for placing a culture dish;
the constant temperature box is connected with the constant temperature layer frame, and a slotted hole for placing the pipe body is formed in the constant temperature box;
the heating module is arranged on the constant-temperature layer frame and used for heating the constant-temperature layer frame;
the refrigeration module is arranged in the constant temperature box and is used for refrigerating the constant temperature box;
and the temperature control module is arranged in the constant temperature box, is connected with the heating module and the refrigerating module and is used for controlling the heating module and the refrigerating module to work.
2. The multi-functional thermostatic stage of claim 1, wherein said thermostatic stage comprises at least two stages, each of said stages having two rows of said notches, said two rows of said notches being of different sizes.
3. The multi-function thermostatic stage of claim 2, wherein at least two of said decks are parallel and equally spaced.
4. The multifunctional thermostatic table of claim 1, wherein said oven is provided with:
a plurality of rows of first slotted holes for placing the freezing storage tubes;
a plurality of rows of second slotted holes for placing centrifuge tubes;
wherein the diameters of the rows of first slotted holes are smaller than the diameters of the rows of second slotted holes.
5. The multifunctional thermostatic table of any one of claims 1 to 4, further comprising a pressure detection assembly, wherein the pressure detection assembly is arranged on the thermostatic shelf and is used for detecting whether a culture dish is placed on the thermostatic shelf.
6. The multifunctional thermostatic table of claim 5, further comprising a time and temperature display control module, wherein the time and temperature display control module is disposed on the thermostatic tank and is connected to the pressure detection assembly and the temperature control module, and the time and temperature display control module is configured to time after a culture dish is placed on the thermostatic shelf and is configured to display a current temperature of the thermostatic shelf.
7. The multifunctional thermostatic table of claim 6, wherein a prompting module is arranged on the thermostatic tank, the prompting module is connected with the time and temperature display control module, and the prompting module gives an alarm prompt after the time and temperature display control module finishes timing so as to remind an operator to take away a culture dish.
8. The multi-functional thermostatic table of claim 1, wherein said thermostatic shelf and said incubator are of a unitary construction.
9. The multifunctional thermostatic table of claim 1, wherein said thermostatic chamber is provided with a power interface for accessing a power source.
10. A biosafety cabinet, comprising a cabinet body and characterized by further comprising a multifunctional constant temperature table which is arranged in the cabinet body and is as claimed in any one of claims 1 to 9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220925011.0U CN217628420U (en) | 2022-04-20 | 2022-04-20 | Biological safety cabinet and multifunctional constant temperature platform thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220925011.0U CN217628420U (en) | 2022-04-20 | 2022-04-20 | Biological safety cabinet and multifunctional constant temperature platform thereof |
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| Publication Number | Publication Date |
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| CN217628420U true CN217628420U (en) | 2022-10-21 |
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| CN202220925011.0U Active CN217628420U (en) | 2022-04-20 | 2022-04-20 | Biological safety cabinet and multifunctional constant temperature platform thereof |
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Address after: 266000 No. 499, qichangcheng Road, Huangdao District, Qingdao, Shandong Patentee after: Qingdao Ruiyuan Cell Biotechnology Development Co.,Ltd. Address before: 266000 No. 499, qichangcheng Road, Huangdao District, Qingdao, Shandong Patentee before: Qingdao Zhongkang yuanneng Cell Biotechnology Co.,Ltd. |
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