CN209054824U - It is a kind of for preventing the alcohol ice chest of nucleic acid renaturation - Google Patents

Abstract

The utility model discloses a kind of for preventing the alcohol ice chest of nucleic acid renaturation, including being contained with the box body of alcoholic solution, the orifice plate at the top of box body to fixed amplified production reaction vessel；Orifice plate has multiple to realize the circular hole that amplified production reaction vessel is fixed with the cooperation of amplified production reaction vessel.Above-mentioned alcohol ice chest cooling rate is fast and maintains the low temperature time long, and nucleic acid renaturation can be prevented in the long period, improves hybridization efficiency when PCR amplification.

Description

It is a kind of for preventing the alcohol ice chest of nucleic acid renaturation

Technical field

The utility model relates to a kind of for preventing the alcohol ice chest of nucleic acid renaturation.

Background technique

When doing the hybrid experiment of amplified production in PCR Lab, needs amplified production to remain DNA single-chain state and be used for With probe hybridization check.

DNA double chain structure can be denaturalized with high temperature (90~100 DEG C) be dissociated into it is single-stranded, it is single-stranded in suitable temperature (30~50 DEG C) renaturation is in conjunction with for DNA double chain, the generation of renaturation in order to prevent, needing to avoid the temperature range of nucleic acid renaturation, (30~50 take the photograph Family name's degree).In order to avoid the temperature range of nucleic acid renaturation, following two methods usually can be used: before hybridization, amplified production being reacted Container is placed in elevated temperature vessel, is maintained high temperature (>=100 DEG C)；Before hybridization, amplified production reaction vessel is placed on low temperature and is held In device.Due to high temperature be unfavorable for personnel operation and there are the risk that high temperature is hurted sb.'s feelings, generally select to using low-temperature (low temperature) vessel to amplification Product reaction vessel is saved.

Therefore, a kind of skill that the low-temperature (low temperature) vessel for preventing nucleic acid renaturation needs to solve as those skilled in the art how is designed Art problem.

Utility model content

The purpose of the utility model is to provide a kind of for preventing the alcohol ice chest of nucleic acid renaturation, for preventing nucleic acid multiple Property, alcohol ice chest cooling velocity is fast, cryogenic hold-time is long, easy to use for this, effectively increases hybridization efficiency.

To achieve the above object, the utility model provides a kind of for preventing the alcohol ice chest of nucleic acid renaturation, including holds There are the box body of alcoholic solution, the orifice plate at the top of the box body to fixed amplified production reaction vessel；

The orifice plate has multiple to realize with the cooperation of amplified production reaction vessel to the progress of amplified production reaction vessel Fixed circular hole.

It preferably, further include bag film to hold alcoholic solution, the bag film is placed in the box body.

Preferably, multiple circular holes with column distribution in the orifice plate, and the circular hole equidistantly distributed of same row, adjacent column The adjacent circular hole be dislocatedly distributed.

Preferably, the box body is the rectangular parallelepiped structure of uncovered, and the orifice plate is the rectangular slab matched with the box body, The orifice plate is fixed at the top of the box body.

Preferably, the side wall at the top of the box body offers the sliding slot to the fixation orifice plate, and the orifice plate is plugged in The sliding slot, which is realized, to be fixed.

Preferably, the circular hole has the cylindric lug boss extended along the vertical hole plate thickness direction.

Preferably, which is characterized in that there are four side walls for the box body tool, and wherein three side walls are contour, are higher than surplus A remaining side wall.

Preferably, the box body is PVC box body.

It is provided by the utility model that nucleic acid product reaction vessel is cooled down using the box body for being contained with alcoholic solution, The freezing point of absolute alcohol namely dehydrated alcohol is -117 DEG C, and far below the freezing point of water, the freezing point of alcoholic solution is also much smaller than 0 DEG C, so that alcoholic solution can be to keep liquid under lower temperature, appearance can sufficiently be reacted with amplified production by meeting cooling requirement Device is contacted, and cooling velocity is accelerated, and using the maintenance low temperature of temperature lower alcoholic solution energy long period, is effectively prevent Nucleic acid renaturation, so that hybridization efficiency is improved, it is convenient that amplified production reaction vessel is consolidated by being provided with the orifice plate of circular hole It is fixed and above-mentioned for preventing the alcohol ice box structure of nucleic acid renaturation simple, easy to use.

Detailed description of the invention

In order to illustrate the embodiment of the utility model or the technical proposal in the existing technology more clearly, below will be to embodiment Or attached drawing needed to be used in the description of the prior art is briefly described, it should be apparent that, the accompanying drawings in the following description is only It is the embodiments of the present invention, for those of ordinary skill in the art, without creative efforts, also Other attached drawings can be obtained according to the attached drawing of offer.

Fig. 1 is provided by the utility model embodiment for preventing the alcohol ice chest schematic diagram of nucleic acid renaturation；

Fig. 2 is in Fig. 1 for preventing the box body schematic diagram of the alcohol ice chest of nucleic acid renaturation；

Fig. 3 is in Fig. 1 for preventing the orifice plate top view of the alcohol ice chest of nucleic acid renaturation；

Fig. 4 is in Fig. 1 for preventing the convex structure figure of the orifice plate of the alcohol ice chest of nucleic acid renaturation.

Specific embodiment

The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without making creative work Every other embodiment obtained, fall within the protection scope of the utility model.

In order to make those skilled in the art more fully understand the utility model, with reference to the accompanying drawing and specifically The utility model is described in further detail for embodiment.

Please refer to figs. 1 to 4, and Fig. 1 is provided by the utility model embodiment for preventing the alcohol ice of nucleic acid renaturation Box schematic diagram；Fig. 2 is in Fig. 1 for preventing the box body schematic diagram of the alcohol ice chest of nucleic acid renaturation；Fig. 3 is in Fig. 1 for preventing The orifice plate top view of the alcohol ice chest of nucleic acid renaturation；Fig. 4 be Fig. 1 in for prevent nucleic acid renaturation alcohol ice chest orifice plate it is convex Play portion's structure chart.

The alcohol ice chest provided by the utility model for being used to prevent nucleic acid renaturation, the box body 1 including being equipped with alcoholic solution, Cooling is carried out to amplified production container and nucleic acid using alcoholic solution subzero characteristic, prevents nucleic acid renaturation；1 top of box body Equipped with the orifice plate 2 for being used to fix amplified production reaction vessel, multiple and amplified production reaction vessel is offered on orifice plate 2 and is matched It closes, to the circular hole 21 of amplified reaction container fixed function.Amplified production reaction vessel is provided with the single stranded DNA of denaturation, passes through The cooling temperature for making single stranded DNA is carried out lower than renaturation temperature (30~50 DEG C) to amplified production container, reaching prevents single stranded DNA multiple Property purpose, improve hybridization efficiency.

Amplified production reaction vessel is conical shell, has circular hole by what setting was matched with amplified production reaction vessel 21 orifice plate 2 can be crossed and fix multiple amplified production reaction vessels, the box body for holding alcoholic solution 1 is made to play cooling effect to it. For guarantee cooling effect, the alcohol ice chest for being used to prevent nucleic acid renaturation is put into -20 DEG C of refrigerator carries out refrigeration cooling in advance, When alcoholic solution is fully cooled, the amplified production reaction vessel equipped with the DNA after denaturation treatment is put into the circular hole 21 of orifice plate 2 Interior fixation can be completed cooling and keep renaturation temperature of the temperature lower than nucleic acid, side easy to operate in amplified production reaction vessel Just；Using alcoholic solution as the reason of coolant liquid be dehydrated alcohol freezing point (- 117 DEG C) it is lower, and by dehydrated alcohol and The freezing point of the alcoholic solution of water configuration is also far below 0 DEG C, this is allowed for, and alcoholic solution can be in liquid form to amplified production Reaction vessel is cooled down, and film-cooled heat is increased, and improves cooling effect, and alcoholic solution is by the temperature far below 0 DEG C (such as -20 DEG C) restores longer to the time of the renaturation temperature of nucleic acid, can keep for a long time low in amplified production reaction vessel Warm situation guarantees that DNA is in the single-chain state for meeting hybridization and requiring, improves hybridization efficiency.

In a kind of specific embodiment provided by the utility model, referring to FIG. 1, for preventing the wine of nucleic acid renaturation Smart ice chest includes the half-headed box body 1 an of rectangular-shape, is provided at the top of box body 1 for fixing amplified production reaction vessel Orifice plate 2, orifice plate 2 be rectangle or rectangular plate structure are matched with the box body 1 of rectangular-shape, offered on orifice plate 2 It is multiple to be used to fix the circular holes 21 of amplified production reaction vessel, it has been contained inside box body 1 and cooling is risen to amplified production reaction vessel Act on alcoholic solution.Inner sidewall at 1 top of box body opens up the sliding slot 11 along side wall circumferentially extending, and what orifice plate 2 can be convenient inserts It is connected to sliding slot 11 and realizes that orifice plate 2 is fixed on the top of box body 1.

To avoid equipped with the alcohol volatilization in alcoholic solution box body 1, in another specific embodiment of the utility model, A kind of bag film 3 is also especially set and holds alcoholic solution, the bag film 3 for being contained with alcoholic solution is placed in box body 1.By thin 3 pairs of alcoholic solutions of film bag are sealed, and effectively prevent the volatilization of alcohol, keep alcohol ice chest provided by the utility model more than enough Secondary use, environment friendly convenient；Alcoholic solution can choose the alcoholic solution that volume fraction is 20%~55%, and the utility model is selected 20% alcoholic solution, freezing point is low, good cooling results, and low temperature is held time length.

The utility model provide 1 structure of box body for preventing the alcohol ice chest of nucleic acid renaturation specifically can as shown in Fig. 2, Box body 1 is the box body 1 of a not no rectangular-shape of 12 × 8 × 5cm of top cover, and the inner sidewall at 1 top of box body opens up useful Carry out the sliding slot 11 of fixed orifice plate 2；Wherein, three side walls of the box body 1 of rectangular-shape are contour and a slightly above remaining side wall Height, specific settable difference in height is 1~2cm, in order to which orifice plate 2 is plugged in sliding slot 11 from the side wall of the lower side of box body 1, Realize the fixation to orifice plate 2；Box body 1 can take PVC plastic to complete the process, and reduce manufacturing cost.

The specific structure is shown in FIG. 3 for orifice plate 2, and orifice plate 2 is to match rectangular plate body, hole with rectangular-shaped box body 1 Multiple circular holes 21 for being used to fix amplified production reaction vessel are offered on plate 2, and amplified production container is plugged in the circle of orifice plate 2 Fixation can be realized in hole 21；In a specific embodiment, 12 × 8 96 circular holes 21 are offered on single orifice plate 2, also 96 amplified production reaction vessel monomers to be fixed；96 circular holes 21 divide 8 column, 12 circular holes 21 of each column and 12 circular holes 21 equidistantly distributeds, the adjacent circular holes 21 between adjacent column are dislocatedly distributed, so that orifice plate 2 is fully used.Amplification can be produced The monomer of object reaction vessel or 12 connecting leg structures are plugged in a column of orifice plate 2.Box body 1 can also use other shapes, on orifice plate 2 Circular hole 21 can also be opened up according to actual needs, as long as guarantee orifice plate 2 and box body 1 cooperation.

The circular hole 21 of orifice plate 2 also has the cylindric lug boss 22 extended along 2 thickness direction of orifice plate, such as Fig. 1 and Fig. 4 institute Show, and then guarantee verticality when amplified production reaction vessel is fixed on orifice plate 2, the height of lug boss 22 adds the thickness of orifice plate 2 Less than the difference in height of three side walls of box body 1 and a remaining side wall, in order to which orifice plate 2 is plugged in sliding slot 11 from lower side wall It realizes and fixes；If the side wall of lower side is lower than its excess-three side wall 2cm, orifice plate 2 is with a thickness of 0.5cm, the setting of lug boss 22 0.5 ~1cm.

For prevent the alcohol ice chest of nucleic acid renaturation box body 1 and 2 shape of orifice plate can flexible setting, size can also basis The specification of container is adjusted.Such as the cylindric box body 1 of uncovered can be used, the circular orifice with the cooperation of cylindric box body 1 2, orifice plate 2 is overlapped in 1 top of box body, the plate body extended downwardly can be arranged in the periphery of circular orifice 2, realizes that the overlap joint of orifice plate 2 is solid It can be opened up along the circumferencial direction of circular orifice 2 due to the circular hole 21 on box body 1, orifice plate 2.

When stating the alcohol ice chest for preventing nucleic acid renaturation in use, orifice plate 2 is taken out, -20 DEG C will be frozen in advance The bag film 3 equipped with alcoholic solution in refrigerator is placed in box body 1, installs fixed orifice plate 2, amplified production reaction vessel is inserted Cooling to amplified production reaction vessel, and the low temperature being maintained for a long time can be completed in the circular hole 21 for being fixed on orifice plate 2, relatively In pure water ice chest and can, good cooling results, cryogenic hold-time are long, to effectively prevent nucleic acid renaturation, improve hybridization Efficiency, and the alcohol ice box structure is simple, it is easy to operate.Cooling effect comparison can refer to following table table 1.

Table 1 is used to prevent the cooling effect table of the alcohol ice chest of nucleic acid renaturation

It should be noted that in the present specification, such as first and second etc relational terms are used merely to one Entity is distinguished with other several entities, and without necessarily requiring or implying between these entities, there are any this actual Relationship or sequence.

Above to provided by the utility model for preventing the alcohol ice chest of nucleic acid renaturation to be described in detail.Herein In apply specific case the principles of the present invention and embodiment be expounded, the explanation of above embodiments is only used The method and its core concept of the utility model are understood in help.It should be pointed out that for those skilled in the art For, without departing from the principle of this utility model, several improvements and modifications can be made to this utility model, these Improvement and modification are also fallen into the protection scope of the utility model claims.

Claims (8)

1. a kind of for preventing the alcohol ice chest of nucleic acid renaturation, which is characterized in that including be contained with alcoholic solution box body (1), Orifice plate (2) at the top of the box body (1) to fixed amplified production reaction vessel；

The orifice plate (2) has multiple to consolidate with the cooperation realization of amplified production reaction vessel to amplified production reaction vessel Fixed circular hole (21).

2. according to claim 1 for preventing the alcohol ice chest of nucleic acid renaturation, which is characterized in that further include to hold The bag film (3) of alcoholic solution, the bag film (3) are placed in the box body (1).

3. according to claim 1 for preventing the alcohol ice chest of nucleic acid renaturation, which is characterized in that multiple circular holes (21) with column distribution in the orifice plate (2), and the circular hole (21) equidistantly distributed of same row, the adjacent circle of adjacent column Hole (21) is dislocatedly distributed.

4. according to claim 3 for preventing the alcohol ice chest of nucleic acid renaturation, which is characterized in that the box body (1) is The rectangular parallelepiped structure of uncovered, the orifice plate (2) are the rectangular slab matched with the box body (1), and the orifice plate (2) is fixed on institute It states at the top of box body (1).

5. according to claim 4 for preventing the alcohol ice chest of nucleic acid renaturation, which is characterized in that box body (1) top The side wall in portion offers the sliding slot (11) to the fixation orifice plate (2), and the orifice plate (2) is plugged in the sliding slot (11) realization It is fixed.

6. according to claim 5 for preventing the alcohol ice chest of nucleic acid renaturation, which is characterized in that circular hole (21) tool There is the cylindric lug boss (22) extended along vertical orifice plate (2) thickness direction.

7. according to claim 6 for preventing the alcohol ice chest of nucleic acid renaturation, which is characterized in that box body (1) tool There are four side walls, and wherein three side walls are contour, are higher than a remaining side wall.

8. according to any one of claims 1 to 7 for preventing the alcohol ice chest of nucleic acid renaturation, which is characterized in that described Box body (1) is PVC box body.