CN207307904U - A kind of molecular biosciences use for laboratory ice-water bath box - Google Patents
A kind of molecular biosciences use for laboratory ice-water bath box Download PDFInfo
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- CN207307904U CN207307904U CN201720725437.0U CN201720725437U CN207307904U CN 207307904 U CN207307904 U CN 207307904U CN 201720725437 U CN201720725437 U CN 201720725437U CN 207307904 U CN207307904 U CN 207307904U
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- pcr reaction
- ice
- water bath
- transparent cartridge
- bath box
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Abstract
The utility model provides a kind of molecular biosciences use for laboratory ice-water bath box, including open-topped transparent cartridge;The transparent cartridge inside points offer water filling port every being placed with several ice making cells at the top of the ice making cell;The transparent cartridge top open part is fixed with PCR reaction pipe supports, and offering several PCR reaction tubes on the PCR reactions pipe support places mouth.It is the compartment for increasing ice making that it, which is mainly improved, so that ice cube is not easy to float;In PCR reaction pipe supports bottom side increase flexible plastic projection, PCR reaction tubes are made to be not easy to float in frozen water, so that time and contacting volume of the sample of nucleic acid being effectively ensured in PCR reaction tubes in ice-water bath, and then effectively maintain the denatured state of nucleic acid.
Description
Technical field
The field of medical instrument technology is the utility model is related to, specifically discloses a kind of molecular biosciences use for laboratory ice-water bath box.
Background technology
In molecule hybrid experiment, for maintain the single-stranded structure of nucleic acid, it is necessary to after nucleic acid denaturation ice-water bath immediately.At present
Laboratory uses the container provided for oneself in refrigerator placement overnight, and water is added after ice making, forms ice-water bath, then adds PCR reactions
Pipe, this method problems faced have:PCR reaction tubes since buoyancy can float in ice-water bath, receive ice-water bath time and
Volume is not abundant enough;Time is slightly long, and ice cube will dissolve floating, and PCR reaction tubes are jacked up, and prevent sample cell from ice-water bath
Dipping bath.
The content of the invention
The purpose of this utility model is to provide a kind of molecular biosciences use for laboratory ice-water bath box, is mainly used for molecular biosciences reality
Test the maintenance of the single nucleic acid strands structure of room.
The technical solution of the utility model is:A kind of molecular biosciences use for laboratory ice-water bath box, including it is open-topped
Bright box body;
The transparent cartridge inside points offer water filling every being placed with several ice making cells at the top of the ice making cell
Mouthful;
The transparent cartridge top open part is fixed with PCR reaction pipe supports, several are offered on the PCR reactions pipe support
PCR reaction tubes place mouth.
Further, be provided with PCR reaction pipe support fixed card buckles at the top of the transparent cartridge, the PCR reaction pipe supports with
PCR reactions pipe support fixed card buckle corresponds to junction and is provided with fixed bayonet, the PCR reaction pipe support fixed card buckles and fixed bayonet
Supporting installation is fixed.It is fixedly connected reliable.
Further, symmetrical two corners are respectively arranged with a PCR reaction pipe support fixed card buckle at the top of the transparent cartridge,
PCR reaction pipe supports junction corresponding with two PCR reactions pipe support fixed card buckles is each provided with a fixed bayonet, described
PCR reacts pipe support fixed card buckle and the supporting installation of fixed bayonet is fixed.Two corners are symmetrically fixed, and are fixedly connected reliable.
Further, the PCR reaction tubes placement mouth, which is circumferentially evenly arranged with several, can clamp PCR reaction tube appearances
The flexible plastic projection in face, several flexible plastic projections are placed mouth central shaft towards PCR reaction tubes and are drawn close.It is anti-PCR can effectively to be fixed
Ying Guan.
Further, the flexible plastic projection places downward 30 °~45 ° of mouth planar tilt with respect to PCR reaction tubes.It is more convenient
Insertion and effectively fixed PCR reaction tubes.
Further, the transparent cartridge is cube structure.
Further, the PCR reaction pipe supports react pipe support for 96 hole 200ulPCR.
Further, the ice making cell has 5, respectively positioned at transparent cartridge inside bottom centre and corner.
Further, the height of the ice making cell is less than 1.5-2.5cm at the top of transparent cartridge, the body of all ice making cells
Product and the half for transparent cartridge volume.
The beneficial effect of the utility model ice-water bath box:
It is the compartment for increasing ice making that it, which is mainly improved, so that ice cube is not easy to float;In PCR reaction pipe supports bottom side
Increase flexible plastic projection, make PCR reaction tubes be not easy to float in frozen water, so that the nucleic acid in PCR reaction tubes be effectively ensured
Time and contacting volume of the sample in ice-water bath, and then effectively maintain the denatured state of nucleic acid.
Brief description of the drawings
Fig. 1 ice-water bath box ice maker container structure diagrams;
Fig. 2 PCR react pipe support schematic diagram;
The anti-seam allowance of PCR reaction tubes of Fig. 3 PCR reaction pipe supports places the structure diagram that mouth carries flexible plastic protrusion.
In figure:1 is transparent cartridge, and 2 be ice making cell, and 3 be water filling port, and 4 react pipe support fixed card buckle for PCR, and 5 be fixation
Bayonet, 6 react pipe support for PCR, and 7 place mouth for PCR reaction tubes, and 8 is raised for flexible plastic.
Embodiment
Structure Figure is described further the utility model below.
As shown in Figure 1, a kind of molecular biosciences use for laboratory ice-water bath box, including open-topped cube transparent cartridge 1.Thoroughly
Bright 1 inside points of box body are every being placed with 5 ice making cells 2, respectively positioned at transparent cartridge inside bottom centre and corner, ice making cell
2 tops offer water filling port 3.The height of ice making cell 2 is less than 1 top 2cm of transparent cartridge, the volume of all ice making cells 2 and
For the half of 1 volume of transparent cartridge.
As shown in Fig. 2, 1 top open part of transparent cartridge is fixed with 96 hole 200ulPCR reaction pipe supports 6, PCR reaction pipe supports 6
On there are 96 PCR reaction tubes to place mouths 7.
As preferred embodiment, the symmetrical two corners in the top of transparent cartridge 1 are respectively arranged with a PCR reaction pipe support and consolidate
Surely 4, the PCR that is buckled reacts pipe support 6 and is each provided with a fixed bayonet with two 4 corresponding junctions of PCR reaction pipe supports fixed card buckle
5, PCR reaction pipe support fixed card buckles 4 and the supporting installation of fixed bayonet 5 are fixed.Two corners are symmetrically fixed, and are fixedly connected reliable.
In order to more effectively fixed PCR reaction tubes, place mouth 7 in PCR reaction tubes and be circumferentially evenly arranged with several energy
The flexible plastic projection 8 of PCR reaction tube outer surfaces is enough clamped, several flexible plastic projections 8 place mouth central shaft towards PCR reaction tubes
Draw close.Likewise it is preferred that, flexible plastic projection 8 places downward 30 °~45 ° of 7 planar tilt of mouth with respect to PCR reaction tubes.More easily
Insertion and effectively fixed PCR reaction tubes.
The method in clinical application of this ice-water bath box:
At least shift to an earlier date 8 it is small when 5 cells of the cuboid plastic containers of ice-water bath box are filled into water, in the refrigerating chamber of refrigerator
Place, treat that whole Cheng Binghou take out, PCR reaction pipe supports are fixed on cuboid plastic containers, then note whole cuboid
Full water, after ten minutes can be placed on it by the PCR reaction tubes containing the sample of nucleic acid being denatured, after placing 5 minutes, you can
To carry out follow-up hybrid experiment.
It is the compartment for increasing ice making that the utility model, which mainly improves, so that ice cube is not easy to float.Reacted in PCR
Pipe support bottom side increases flexible plastic projection, makes PCR reaction tubes be not easy to float in frozen water, so that PCR reaction tubes be effectively ensured
In time and contacting volume of the sample of nucleic acid in ice-water bath, and then effectively maintain the denatured state of nucleic acid.
It the above is only the preferred embodiment of the present invention, it is noted that come for those skilled in the art
Say, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications also should be regarded as
Protection scope of the present invention.
Claims (9)
- A kind of 1. molecular biosciences use for laboratory ice-water bath box, it is characterised in that:Including open-topped transparent cartridge (1);Transparent cartridge (1) inside points are offered every being placed with several ice making cells (2) at the top of the ice making cell (2) Water filling port (3);Transparent cartridge (1) top open part is fixed with PCR reaction pipe supports (6), is offered on the PCR reaction pipe supports (6) Several PCR reaction tubes place mouth (7).
- A kind of 2. molecular biosciences use for laboratory ice-water bath box according to claim 1, it is characterised in that:The transparent cartridge (1) top is provided with PCR reaction pipe support fixed card buckles (4), the PCR reaction pipe supports (6) and PCR reaction pipe support fixed card buckles (4) corresponding junction is provided with fixed bayonet (5), the PCR reaction pipe support fixed card buckles (4) and fixed bayonet (5) supporting peace Dress is fixed.
- A kind of 3. molecular biosciences use for laboratory ice-water bath box according to claim 2, it is characterised in that:The transparent cartridge (1) the symmetrical two corners in top are respectively arranged with a PCR reaction pipe support fixed card buckle (4), the PCR reaction pipe supports (6) and two A corresponding junction of PCR reaction pipe support fixed card buckles (4) is each provided with a fixed bayonet (5), and the PCR reactions pipe support is fixed It is buckled (4) and the supporting installation of fixed bayonet (5) is fixed.
- A kind of 4. molecular biosciences use for laboratory ice-water bath box according to claim 1, it is characterised in that:The PCR reactions Pipe placement mouth (7), which is circumferentially evenly arranged with several, can clamp the flexible plastic projection (8) of PCR reaction tube outer surfaces, several Flexible plastic projection (8) is placed mouth central shaft towards PCR reaction tubes and is drawn close.
- A kind of 5. molecular biosciences use for laboratory ice-water bath box according to claim 4, it is characterised in that:The flexible plastic is dashed forward Play (8) and place downward 30 °~45 ° of mouth (7) planar tilt with respect to PCR reaction tubes.
- A kind of 6. molecular biosciences use for laboratory ice-water bath box according to claim 1, it is characterised in that:The transparent cartridge (1) it is cube structure.
- A kind of 7. molecular biosciences use for laboratory ice-water bath box according to claim 1, it is characterised in that:The PCR reactions Pipe support (6) reacts pipe support for 96 hole 200ulPCR.
- A kind of 8. molecular biosciences use for laboratory ice-water bath box according to claim 1, it is characterised in that:The ice making cell (2) there are 5, respectively positioned at transparent cartridge inside bottom centre and corner.
- A kind of 9. molecular biosciences use for laboratory ice-water bath box according to claim 1, it is characterised in that:The ice making cell (2) height is less than 1.5-2.5cm at the top of transparent cartridge (1), the volume of all ice making cells (2) and be transparent cartridge (1) body Long-pending half.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201720725437.0U CN207307904U (en) | 2017-06-21 | 2017-06-21 | A kind of molecular biosciences use for laboratory ice-water bath box |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CN201720725437.0U CN207307904U (en) | 2017-06-21 | 2017-06-21 | A kind of molecular biosciences use for laboratory ice-water bath box |
Publications (1)
Publication Number | Publication Date |
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CN207307904U true CN207307904U (en) | 2018-05-04 |
Family
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CN201720725437.0U Active CN207307904U (en) | 2017-06-21 | 2017-06-21 | A kind of molecular biosciences use for laboratory ice-water bath box |
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CN (1) | CN207307904U (en) |
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2017
- 2017-06-21 CN CN201720725437.0U patent/CN207307904U/en active Active
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