CN206986163U - A kind of 96 orifice plates for being used to preserve reagent seal up plate film - Google Patents
A kind of 96 orifice plates for being used to preserve reagent seal up plate film Download PDFInfo
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- CN206986163U CN206986163U CN201720913653.8U CN201720913653U CN206986163U CN 206986163 U CN206986163 U CN 206986163U CN 201720913653 U CN201720913653 U CN 201720913653U CN 206986163 U CN206986163 U CN 206986163U
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Abstract
The utility model discloses a kind of 96 orifice plates for being used to preserve reagent to seal up plate film, include sealing up plate successively from top to bottom and be laid on the aerated film for sealing up plate bottom and tempering film, tempering film is arranged to the arc spherical structure of indent, aerated film and tempering film connect through inert gas relatively and fill to form spindle cavity, and the both ends of spindle cavity, which stretch out, is provided with inflating balloon；Seal up plate and be arranged to the upper sealing plate of horizontal parallel arrangement and lower shrouding, domed transparent shell is embedded with upper sealing plate, the outstanding suspension for setting ball float, lower shrouding and the tangent connection of aerated film are filled with hyaline test；Plate is sealed up to be fastened on 96 orifice plates by tempering film.Seal up plate film and play a part of support balance, bumper and absorbing shock, can control the line stability of reagent in 96 orifice plates.The mode that more person-portion reagents are filled with 1.5ml or 2.0ml centrifuge tubes is changed to the mode dispensed in advance with 96 orifice plates, can effectively prevent remaining reagent to be contaminated and degrade.
Description
Technical field
The utility model belongs to detection device technical field, and in particular to a kind of 96 orifice plates for being used to preserve reagent seal up plate
Film.
Background technology
Fluorescent quantitative PCR technique be grow up based on traditional PCR technique and with reference to spectral technique a kind of it is sensitiveer,
More special, more accurate nucleic acid detection technique.Testing result is accurate, and repeatability is high, and dynamic reaction patient treats forward and backward cause of disease
Body dynamic change and the relation with clinic, and the problem of normal PCR need to post-process is avoided in whole process, reduce pollution.
Real-Time Fluorescent Quantitative PCR Technique uses a kind of PCR amplification instrument with fluorescence detection device, and fluorescence detection device can be according to one
The exciting light of specific wavelength is sent to fixed routines periodically, detection fluorescence signal is collected, by the dynamic for detecting fluorescence signal
The level of amplification of change reflection PCR each circulation in real time, can automatically analyze acquisition amplification curve by software after off-test,
According to amplification curve and the intersection point (i.e. Ct values) of fluorescence threshold line and the shape of amplification curve, it can be determined that yin and yang attribute result；
If having the qualitative reference product or standard items of concentration known in same reaction, acquisition standard song can be automatically analyzed by software
Line, it is achieved in the definite value (i.e. quantitative detection) to unknown sample.
Kit is widely used in fluorescent quantitative PCR detection method at present, but existing kit is by more person-portions
Reagent reacting liquid is centrally saved in the mode of a centrifuge tube, if be used for multiple times, is limited to preservation condition, reagent can multigelation
For several times, the degraded of primed probe in reagent is caused, influences the collection of fluorescence signal.
Utility model content
For problems of the prior art, the purpose of this utility model, which is to provide, a kind of to be used to preserve the 96 of reagent
Orifice plate seals up plate film.
The technical scheme that the utility model is taken is：
A kind of 96 orifice plates for being used to preserve reagent seal up plate film, include sealing up plate and being laid on sealing up successively from top to bottom
The aerated film and tempering film of plate bottom, tempering film are arranged to the arc spherical structure of indent, aerated film and the relative connection of tempering film
Fill to form spindle cavity by inert gas, the both ends of spindle cavity, which stretch out, is provided with inflating balloon；It is described to add
Shrouding is arranged to the upper sealing plate of horizontal parallel arrangement and lower shrouding, is embedded with domed transparent shell in upper sealing plate, is filled out in hyaline test
Filled with the outstanding suspension for setting ball float, lower shrouding and the tangent connection of aerated film；Plate is sealed up to be fastened on 96 orifice plates by tempering film.
Further, the lower shrouding is arranged to the structure that section is formed by connecting in the dividing plate circulation of inverted trapezoidal.
Further, the lower surface of the tempering film and the tangent arrangement of through hole hole wall of 96 orifice plates.
Further, the inflating balloon is set with the tangent arrangement in the lower surface for sealing up plate, and the diameter of inflating balloon is less than
The maximum gauge of spindle cavity.
Further, the aerated film is prepared using elastic rubber material, the song of curvature >=tempering film of aerated film
Rate.
Further, the diameter of the ball float is less than the radial height of hyaline test, and the liquid level of suspension is the 1/ of hyaline test
2-2/3。
Further, for the plate of sealing up in when being placed in parallel, ball float is suspended in the center of hyaline test.
The beneficial effects of the utility model are：
Tempering film in the utility model plays the synergy of support balanced action, aerated film and inflating balloon, plays
The effect of bumper and absorbing shock, by the degree of balance of ball float Real Time Observation upper sealing plate, and then ensure the liquid level stabilizing of reagent in 96 orifice plates
Property.The mode that the mode that more person-portion reagents are filled with 1.5ml or 2.0ml centrifuge tubes is changed to be dispensed in advance with 96 orifice plates is, it is necessary to use
When, 96 orifice plates of pre- reagent adding are taken out, the DNA for adding sample to be detected per hole to room temperature is balanced, light is covered with again after of short duration centrifugation
Learn machine testing on shrouding film.Remaining reagent can be effectively prevented to be contaminated and degrade.
Brief description of the drawings
Fig. 1 is the 96 AND DEWATERING FOR ORIFICE STRUCTURE schematic diagrames that band seals up plate film in the utility model；
Fig. 2 is the 96 orifice plate schematic cross-sections that band seals up plate film in the utility model；
Fig. 3 is the reagent cartridge configuration schematic diagram with Reagent Tube in the utility model；
Wherein, 1,96 orifice plate；2nd, plate film is sealed up；3rd, tempering film；4th, aerated film；5th, inflating balloon；6th, dividing plate；7th, it is transparent
Shell；8th, suspension；9th, ball float；10th, kit；11st, Reagent Tube.
Embodiment
The utility model is further illustrated below in conjunction with the accompanying drawings.
Embodiment 1
As depicted in figs. 1 and 2, a kind of 96 orifice plates for being used to preserve reagent seal up plate film, include sealing up from top to bottom successively
Plate and the aerated film 4 and tempering film 3 for sealing up plate bottom are laid on, tempering film 3 is arranged to the arc spherical structure of indent, inflation
Film 4 and tempering film 3 connect through inert gas relatively and fill to form spindle cavity, seal up plate and are arranged to horizontal parallel arrangement
Upper sealing plate and lower shrouding.
Specifically used process is：As shown in figure 3, according to the explanation of the specification reagent preparation process of kit 10, according to every
Every kind of reaction solution in Reagent Tube 11 in kit 10 is added separately in 96 orifice plates 1 by hole dispensed loading amount, and it is horizontal will to seal up plate film 2
It is placed on the through hole of 96 orifice plates 1, the lower surface of tempering film 3 and the tangent arrangement of through hole hole wall of 96 orifice plates 1, is sealed up with shrouding film
96 orifice plates 1 are placed in -20 DEG C of preservations to prevent the pollution of reagent.When needing to use, 96 orifice plates 1 of pre- reagent adding are taken out, balance is extremely
Room temperature, the DNA of sample to be detected is added per hole, is covered with machine testing on optics shrouding film after of short duration centrifugation again.1.5ml will be used
Or 2.0ml centrifuge tubes fill the modes of more person-portion reagents and are changed to the mode dispensed in advance with 96 orifice plates 1, can effectively prevent remaining reagent by
To pollution and degraded.
The tempering film 3 of the lower surface of spindle cavity in the utility model plays support balanced action, and upper surface is filled
Air film 4 is prepared using elastic rubber material, the curvature of curvature >=tempering film 3 of aerated film 4, has certain elasticity, is risen
To the effect of bumper and absorbing shock, the both ends of spindle cavity, which stretch out, is provided with inflating balloon 5, and inflating balloon 5 is with sealing up plate
The tangent arrangement in lower surface is set, and the diameter of inflating balloon 5 is less than the maximum gauge of spindle cavity.Seal up plate and the phase of 96 orifice plate 1
When cutting placement, inflating balloon 5 is fastened on the dividing plate 6 on 96 orifice plates 1 between adjacent through-holes, is had necessarily by sealing up plate itself
Weight, seal up plate and produce certain elastic pressure with inflating balloon 5, aerated film 4, be advantageous to increase contact area, play buffering
With the effect of sealing.
Domed transparent shell 7 is embedded with upper sealing plate, the outstanding suspension 8 for setting ball float 9 is filled with hyaline test 7, ball float 9
Diameter is less than the radial height of hyaline test 7, and the liquid level of suspension 8 is the 1/2-2/3 of hyaline test 7.Put when sealing up plate in parallel
When putting, ball float 9 is suspended in the center of hyaline test 7；When sealing up plate and being not at being placed in parallel, ball float 9 is suspended in hyaline test 7
Lateral location；Pass through the position where ball float 9, the degree of balance of Real Time Observation upper sealing plate, so as to keep the horizontal of 96 orifice plates 1
State, and then ensure the line stability of reagent in 96 orifice plates 1.
Lower shrouding is arranged to section and the structure being formed by connecting is circulated in the dividing plate 6 of inverted trapezoidal, and lower shrouding and aerated film 4 are tangent
Connection.While the dividing plate 6 of lower shrouding serves stable supportive, processing cost is fully saved, is easy to production scale.
Described above is not to limitation of the present utility model, it should be pointed out that：For the ordinary skill people of the art
For member, on the premise of the utility model in essence scope is not departed from, some variations, modifications, additions or substitutions can also be made,
These improvements and modifications also should be regarded as the scope of protection of the utility model.
Claims (7)
1. it is a kind of be used to preserving 96 orifice plates of reagent seal up plate film, it is characterised in that include sealing up from top to bottom successively plate and
The aerated film for sealing up plate bottom and tempering film are laid on, tempering film is arranged to the arc spherical structure of indent, aerated film and tempering
Film connects through inert gas relatively and fills to form spindle cavity, and the both ends of spindle cavity, which stretch out, is provided with inflatable ball
Capsule；The plate of sealing up is arranged to the upper sealing plate of horizontal parallel arrangement and lower shrouding, is embedded with domed transparent shell in upper sealing plate, thoroughly
Filled with the outstanding suspension for setting ball float, lower shrouding and the tangent connection of aerated film in bright shell；Seal up plate and be fastened on 96 by tempering film
On orifice plate.
2. a kind of 96 orifice plates for being used to preserve reagent seal up plate film according to claim 1, it is characterised in that the lower shrouding
It is arranged to the structure that section is formed by connecting in the dividing plate circulation of inverted trapezoidal.
3. a kind of 96 orifice plates for being used to preserve reagent seal up plate film according to claim 1, it is characterised in that the tempering film
Lower surface and 96 orifice plates the tangent arrangement of through hole hole wall.
4. a kind of 96 orifice plates for being used to preserve reagent seal up plate film according to claim 1, it is characterised in that the inflatable ball
Capsule and the tangent arrangement setting in lower surface for sealing up plate, the diameter of inflating balloon are less than the maximum gauge of spindle cavity.
5. a kind of 96 orifice plates for being used to preserve reagent seal up plate film according to claim 1, it is characterised in that the aerated film
It is prepared using elastic rubber material, the curvature of curvature >=tempering film of aerated film.
6. a kind of 96 orifice plates for being used to preserve reagent seal up plate film according to claim 1, it is characterised in that the ball float
Diameter is less than the radial height of hyaline test, and the liquid level of suspension is the 1/2-2/3 of hyaline test.
7. a kind of 96 orifice plates for being used to preserve reagent seal up plate film according to claim 1, it is characterised in that described to seal up plate
In when being placed in parallel, ball float is suspended in the center of hyaline test.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720913653.8U CN206986163U (en) | 2017-07-26 | 2017-07-26 | A kind of 96 orifice plates for being used to preserve reagent seal up plate film |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720913653.8U CN206986163U (en) | 2017-07-26 | 2017-07-26 | A kind of 96 orifice plates for being used to preserve reagent seal up plate film |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206986163U true CN206986163U (en) | 2018-02-09 |
Family
ID=61416869
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720913653.8U Active CN206986163U (en) | 2017-07-26 | 2017-07-26 | A kind of 96 orifice plates for being used to preserve reagent seal up plate film |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206986163U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114032175A (en) * | 2021-11-02 | 2022-02-11 | 美东汇成生命科技(昆山)有限公司 | A shrouding membrane that is used for 96 hole reaction plates of full shirt rim to seal |
| CN114316826A (en) * | 2021-11-16 | 2022-04-12 | 美东汇成生命科技(昆山)有限公司 | Shrouding membrane that practicality is high |
| CN114958966A (en) * | 2022-07-05 | 2022-08-30 | 济凡生物科技（北京）有限公司 | Kit for high-throughput determination of concentration of double-stranded DNA and application thereof |
| WO2023065418A1 (en) * | 2021-10-20 | 2023-04-27 | 美东汇成生命科技(昆山)有限公司 | Pcr plate-sealing film having good sealing effect |
-
2017
- 2017-07-26 CN CN201720913653.8U patent/CN206986163U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023065418A1 (en) * | 2021-10-20 | 2023-04-27 | 美东汇成生命科技(昆山)有限公司 | Pcr plate-sealing film having good sealing effect |
| CN114032175A (en) * | 2021-11-02 | 2022-02-11 | 美东汇成生命科技(昆山)有限公司 | A shrouding membrane that is used for 96 hole reaction plates of full shirt rim to seal |
| CN114316826A (en) * | 2021-11-16 | 2022-04-12 | 美东汇成生命科技(昆山)有限公司 | Shrouding membrane that practicality is high |
| CN114958966A (en) * | 2022-07-05 | 2022-08-30 | 济凡生物科技（北京）有限公司 | Kit for high-throughput determination of concentration of double-stranded DNA and application thereof |
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| CP01 | Change in the name or title of a patent holder |
Address after: 266000 floor 6, No. 168, Zhuzhou Road, Laoshan District, Qingdao City, Shandong Province Patentee after: Qingdao Renhe medical laboratory Co.,Ltd. Address before: 266000 floor 6, No. 168, Zhuzhou Road, Laoshan District, Qingdao City, Shandong Province Patentee before: QINGDAO JIEMA MEDICAL EXAMINATION Co.,Ltd. |