CN204325344U - The detection chip of cell iophenoxic acid naphthols esterase - Google Patents
The detection chip of cell iophenoxic acid naphthols esterase Download PDFInfo
- Publication number
- CN204325344U CN204325344U CN201420775503.1U CN201420775503U CN204325344U CN 204325344 U CN204325344 U CN 204325344U CN 201420775503 U CN201420775503 U CN 201420775503U CN 204325344 U CN204325344 U CN 204325344U
- Authority
- CN
- China
- Prior art keywords
- chip
- cell
- nido
- framework
- cell capture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to micro-current controlled cell chip technical field, discloses a kind of detection chip measuring cell iophenoxic acid naphthols esterase.Be embodied in, cell chip structure contains cell microfluidic control passage, and in cell is by process, there is cell capture nest.The beneficial effects of the utility model are: the nido structure of this chip effectively can catch cell, make detection easy, quick, stable.
Description
Technical field
The utility model relates to biomedical sector, has design biochip.
Technical background
Cell chip is a kind of biochip technology using cell as research object, and it is the emerging technology adapting to gene, the molecule epoch produce for exploring life science demand.Cell chip not only keep the advantage of tradition research cellular processes as but also meet the features such as high-throughput, large sample and quick obtaining cellular informatics.
The purpose of this utility model there are provided one can catch specific cells, and before can effectively overcoming, chip can measure the shortcoming effectively can not catching cell type specific expression bioinformation in cell biological process.
Summary of the invention
The technical problem that the utility model solves overcomes utilizing chip technology to measure in cell α-naphthylacetate enzyme process because body fluid cell type complicated difficult is with Accurate Determining cell expressing α-naphthylacetate enzymic activity.
Cell α-naphthyl acetate esterase detection chip part comprise chip upper cover (2), upper nido cell capture framework (3), lower nido cell capture framework (4), chip pad (5) and input, output channel.Chi frame is that macromolecule polymeric material is made, and the material of die substrate is any one in glass, macromolecule polymeric material, cellulose membrane, quartz slide.The framework of the upper attached all round closure of chip pad (5), the height of all round closure framework is 3mm.The upper end of closed frame is connected with chip upper cover (2), between chip pad (5) and chip upper cover (2), inlay nido cell capture framework (3), lower nido cell capture framework (4).Chip covers with solution input channel (1), chip pad is with solution output channel (6).
Chip covers the solution input channel (1) had, the shape of passage is circular pipe or square pipe, the length of side of pipe diameter or square pipe is 8mm, utilizes tackiness agent by both compact siro spinning technology, form enclosed construction between pipeline and chip upper cover.Chip upper cover and solution input channel also can be set to integrated structure.
Be embedded on upper nido cell capture framework (3) between chip upper cover and chip pad and lower nido cell capture framework (4) and have the spherical sunk structure of semicircle, form nido cell capture structure.Upper nido cell capture framework (3) has the short block of 2 hemispherical depression containing a long block with 4 hemispherical depression and two, and short block is symmetrically set in the two ends of long block, and the distance of block between block is 50nm; Lower nido cell capture framework (4) has the long block of 4 hemispherical depression containing two, be closely connected between two pieces.
With solution output channel (6) in chip pad, the shape of passage is circular pipe or square pipe, the length of side of pipe diameter or square pipe is 10mm, utilize tackiness agent by both compact siro spinning technology between pipeline and chip upper cover, form enclosed construction, chip pad and solution output channel also can be set to integrated structure.
The nido structure of this chip effectively can catch cell, makes detection easier, quick, stable.
Accompanying drawing explanation
Fig. 1 is the schematic diagram of chip
Fig. 2 chip schematic side view
Fig. 3 chip upper cover schematic diagram
Nido cell capture block schematic illustration on Fig. 4 chip
Nido cell capture block schematic illustration under Fig. 5 chip
Fig. 6 chip pad schematic diagram
Embodiment
A kind of detection chip measuring cell iophenoxic acid naphthols esterase that the utility model provides, explains invention further with specific embodiment below, but does not limit the scope of the invention.
See accompanying drawing 1-6, cell α-naphthyl acetate esterase detection chip of the present invention is primarily of chip upper cover (2), upper nido cell capture framework (3), lower nido cell capture framework (4), chip pad (5) and solution input channel (1), solution output channel (6) composition.Chip can adopt macromolecule polymeric material, and base adopts glass material.
Cell α-naphthyl acetate esterase detection chip part comprise chip upper cover (2), upper nido cell capture framework (3), lower nido cell capture framework (4), chip pad (5) and input, output channel.The framework of the upper attached all round closure of chip pad (5), the height of all round closure framework is 3mm.The upper end of closed frame is connected with chip upper cover (2), between chip pad (5) and chip upper cover (2), inlay nido cell capture framework (3), lower nido cell capture framework (4).
Chip covers with solution input channel (1), the shape of passage is circular pipe, and pipe diameter is 8mm, utilizes tackiness agent by both compact siro spinning technology, form enclosed construction between pipeline and chip upper cover.
Be embedded on upper nido cell capture framework (3) between chip upper cover and chip pad and lower nido cell capture framework (4) and have the spherical sunk structure of semicircle, form nido cell capture structure.Upper nido cell capture framework (3) has the short block of 2 hemispherical depression containing a long block with 4 hemispherical depression and two, and short block is symmetrically set in the two ends of long block, and the distance of block between block is 50nm; Lower nido cell capture framework (4) has the long block of 4 hemispherical depression containing two, be closely connected between two pieces.
With solution output channel (6) in chip pad, the shape of passage is circular pipe, and pipe diameter is 10mm, utilizes tackiness agent by both compact siro spinning technology, form enclosed construction between pipeline and chip upper cover.
Above embodiment of the present utility model has been described in detail, but described content being only preferred embodiment of the present utility model, can not being considered to for limiting practical range of the present utility model.All equalization changes done according to the utility model application range with improve, within the patent covering scope that still all should belong to utility model.
Claims (7)
1. measure the detection chip of cell iophenoxic acid naphthols esterase, it is characterized in that: described chip comprises chip upper cover, upper nido cell capture framework, lower nido cell capture framework, chip pad and input, output channel, chip cover with solution input channel, chip pad is with solution output channel, between chip pad and chip upper cover, inlay nido cell capture framework, lower nido cell capture framework, the framework of attached all round closure in described chip pad, the upper end of closed frame is connected with chip upper cover, be embedded on upper nido cell capture framework between chip upper cover and chip pad and lower nido cell capture framework and have the spherical sunk structure of semicircle, form nido cell capture structure.
2. the detection chip of mensuration cell iophenoxic acid naphthols esterase according to claim 1, it is characterized in that: the material of chi frame is macromolecule polymeric material, the material of die substrate is any one in glass, macromolecule polymeric material, cellulose membrane, quartz slide.
3. the detection chip of mensuration cell iophenoxic acid naphthols esterase according to claim 1, is characterized in that: the height of described all round closure framework is 3mm.
4. the detection chip of mensuration cell iophenoxic acid naphthols esterase according to claim 1, is characterized in that:
Chip covering the solution input channel had is circular pipe, and pipe diameter is 8mm, utilizes tackiness agent by both compact siro spinning technology, form enclosed construction between pipeline and chip upper cover; With solution output channel in chip pad, the shape of passage is circular pipe, and pipe diameter is 10mm, utilizes tackiness agent by both compact siro spinning technology, form enclosed construction between pipeline and chip pad.
5. the detection chip of mensuration cell iophenoxic acid naphthols esterase according to claim 1, is characterized in that:
Chip covering the solution input channel had is square pipe, and the length of side of square pipe is 8mm, utilizes tackiness agent by both compact siro spinning technology, form enclosed construction between pipeline and chip upper cover; With solution output channel in chip pad, the shape of passage is square pipe, and the length of side of square pipe is 10mm, utilizes tackiness agent by both compact siro spinning technology, form enclosed construction between pipeline and chip pad.
6. the detection chip of the mensuration cell iophenoxic acid naphthols esterase according to claim 4 or 5, is characterized in that: the structure that chip upper cover and solution input channel are formed in one; The structure that chip pad and solution output channel are formed in one.
7. the detection chip of mensuration cell iophenoxic acid naphthols esterase according to claim 1, it is characterized in that: upper nido cell capture framework contains the short block that a long block with 4 hemispherical depression and two have 2 hemispherical depression, and short block is symmetrically set in the two ends of long block, the distance of block between block is 50nm; Lower nido cell capture framework contains the long block that two have 4 hemispherical depression, is closely connected between two pieces.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420775503.1U CN204325344U (en) | 2014-12-11 | 2014-12-11 | The detection chip of cell iophenoxic acid naphthols esterase |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420775503.1U CN204325344U (en) | 2014-12-11 | 2014-12-11 | The detection chip of cell iophenoxic acid naphthols esterase |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204325344U true CN204325344U (en) | 2015-05-13 |
Family
ID=53161722
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420775503.1U Active CN204325344U (en) | 2014-12-11 | 2014-12-11 | The detection chip of cell iophenoxic acid naphthols esterase |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204325344U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105651579A (en) * | 2015-12-07 | 2016-06-08 | 上海太阳生物技术有限公司 | Alpha-naphthol butyrate esterase staining kit |
-
2014
- 2014-12-11 CN CN201420775503.1U patent/CN204325344U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105651579A (en) * | 2015-12-07 | 2016-06-08 | 上海太阳生物技术有限公司 | Alpha-naphthol butyrate esterase staining kit |
| CN105651579B (en) * | 2015-12-07 | 2018-10-23 | 上海太阳生物技术有限公司 | A kind of iophenoxic acid naphthol ester enzyme staining reagent kit |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ATE464834T1 (en) | SENSOR WITH IMPROVED BIOCOMPATIBILITY | |
| CN102836751A (en) | Hydrogel micro-fluidic chip and processing method thereof | |
| EP3486645A3 (en) | Analyte sensor | |
| WO2006117422A3 (en) | Method of preparing micro- and nanometric particles with labile products | |
| CN103898058B (en) | A kind of three-dimensional culture method of novel gum knurl stem cell and its application | |
| SG178581A1 (en) | Reversible current gel electrophoresis device for separating biological macromolecules | |
| WO2022148114A1 (en) | Sensor for detecting plant active small molecules and manufacturing method | |
| CN204325344U (en) | The detection chip of cell iophenoxic acid naphthols esterase | |
| CN202099277U (en) | Simulated rumen in vitro fermentation gas producing device | |
| IL245163B (en) | Kit and method for collecting body fluid for medical diagnosis | |
| CN204198745U (en) | A kind of multifunctional unit based on micro-fluidic chip is analyzed porous cell and is cultivated chip | |
| EP3805357A3 (en) | Cell culture flasks, sensor inserts, and systems and methods comprising the same | |
| CN202152343U (en) | Artificial rumen in-vitro fermentation gas-producing device | |
| CN209695205U (en) | A kind of peripheral blood collecting tube of Anti-solidification | |
| CN203448119U (en) | Transfer pipette for experiments on microbiology | |
| CN206489076U (en) | A kind of Novel ATP fluoroscopic examination swab | |
| CN206601394U (en) | A kind of Medical Devices of hand-held quick detection biochemical indicator | |
| CN207376045U (en) | A kind of cell cryopreservation device based on micro-fluidic chip | |
| CN208780672U (en) | A kind of gel release oxygen detection device | |
| CN201959809U (en) | Test tube for blood inspection | |
| CN207540824U (en) | Seismological observation underground water-sampling device | |
| CN202516585U (en) | Blood checkbox type test tube rack | |
| CN202421115U (en) | Multi-index biological capacitance sensor | |
| CN202024944U (en) | Multi-passage blood viscosity measuring device | |
| CN207330914U (en) | A kind of semicolumn Contiuum type Oxford cup |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |