CN101849535A - Portable fish sperm frozen cooling device - Google Patents
Portable fish sperm frozen cooling device Download PDFInfo
- Publication number
- CN101849535A CN101849535A CN201010189738A CN201010189738A CN101849535A CN 101849535 A CN101849535 A CN 101849535A CN 201010189738 A CN201010189738 A CN 201010189738A CN 201010189738 A CN201010189738 A CN 201010189738A CN 101849535 A CN101849535 A CN 101849535A
- Authority
- CN
- China
- Prior art keywords
- floating block
- cover plate
- housing
- spiral shell
- cooling device
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000251468 Actinopterygii Species 0.000 title claims abstract description 20
- 238000001816 cooling Methods 0.000 title claims abstract description 19
- 239000007788 liquid Substances 0.000 claims abstract description 35
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 34
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 17
- 239000000463 material Substances 0.000 claims abstract description 12
- 238000005138 cryopreservation Methods 0.000 claims abstract description 6
- 210000000582 semen Anatomy 0.000 claims description 12
- 239000002184 metal Substances 0.000 claims description 11
- 229910001220 stainless steel Inorganic materials 0.000 claims description 10
- 239000010935 stainless steel Substances 0.000 claims description 10
- 229920003023 plastic Polymers 0.000 claims description 5
- 239000004033 plastic Substances 0.000 claims description 5
- 239000004744 fabric Substances 0.000 claims description 3
- 238000012856 packing Methods 0.000 claims description 3
- 238000007710 freezing Methods 0.000 abstract description 10
- 230000008014 freezing Effects 0.000 abstract description 10
- 238000005070 sampling Methods 0.000 abstract description 3
- 230000007547 defect Effects 0.000 abstract 1
- 235000019688 fish Nutrition 0.000 description 11
- 238000000034 method Methods 0.000 description 6
- 238000004321 preservation Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a portable fish sperm frozen cooling device which comprises a screw axis, a casing, a floating block, a hanging ring, a cover plate and a screw block. The casing is in the shape of a cylindrical cup, a lowest liquid level line and a highest liquid level line of liquid nitrogen loading are marked at the lower part of the inner wall. The middle lower part in the casing is provided with the cylinder floating block; the top part of the floating block is provided with the hanging ring supported by landing legs, and the upper part is provided with the cover plate which can fix the position of a sperm freezing tube; the screw axis passes through the central holes of the hanging ring, the cover plate and the floating block and is vertically positioned in the casing, and the lower end of the screw axis is fixed at the bottom of the casing; and the screw block is assembled on a screw rod of the upper end of the screw axis, and both sides of the crew block are respectively provided with a locking bolt. The invention has reasonable concept, elaborate structure, convenient operation and convenient carrying and use, can effectively overcome the defects of big frozen error and bad repeatability by eyeballing according to liquid nitrogen liquid level, is suitable for sperm sampling two-step freezing of wild fish, and is also suitable for researchers to carry out laboratory precision research of cooling cryopreservation on fish sperm and other materials.
Description
Technical field:
The invention belongs to fish germ plasma resource ultralow temperature and preserve equipment, is a kind of portable fish sperm freezing programmed cooling instrument.
Fish sperm is frozen for a long time, and fish germ plasma resource is preserved and the aquaculture of improved seeds has far-reaching theory significance and using value.To the freezing preservation of some important economic fishs and endangered fish sperm, mainly adopt easy two-step method, multistep processes and programmed cooling method at present.The above two are finished to the distance of liquid nitrogen liquid level by the frozen pipe of artificial manually control seminal fluid usually, will rely on operator's experience in operating process to a great extent, therefore often exist observe difficult, error is big, the repeated shortcoming such as low of refrigerating effect; The latter then needs complexity, expensive programmed cooling instrument to realize, such as the silent program control cooling instrument of CryoMed Freezers that flies your company's production of generation of U.S.'s match, its principle is to utilize pump and the flow velocity of solenoid control liquid nitrogen in cooling recirculation system to reach the purpose of programmed cooling, because it has passed through the reliable control of computer programming controller, thereby can realize accurately freezing from the cell to embryo; But its shortcoming be complex structure, bulky, equipment is heavy, price is high, and be subjected to constraints such as instruments weight, size and be difficult to the field taking a sample to research.
Summary of the invention
The purpose of this invention is to provide a kind of portable fish sperm freezing programmed cooling instrument, to satisfy the needs of the accurate research of open-air sampling and laboratory.
Its structure is to be made of spiral shell axle, housing, floating block, handle, cover plate, spiral shell piece; Housing is that cylinder is cup-shaped, and height is 30-60cm, and diameter is 25-50cm, and inwall below indicates the minimum of the liquid nitrogen of packing into and high liquid level (HLL) line; The middle and lower part is provided with the cylinder floating block in the housing, and thickness is 7-10cm; Diameter 22-48cm; The top of floating block is provided with the handle that is supported by supporting leg, the equipped cover plate that makes spermatozoa cryopreservation pipe fixed-site in top; The spiral shell axle is by the vertical housing that is positioned at of centre bore of handle, cover plate and floating block, and the bottom of housing is fixed in the lower end; The spiral shell piece is the long column body, is fitted on the screw rod of spiral shell axle upper end, each side is provided with a lock screw.
Minimum and the high liquid level (HLL) line of the liquid nitrogen that described container inner wall below is provided with, minimum level line-spacing housing bottom surface is 7cm, high liquid level (HLL) line-spacing housing bottom surface is 12cm.
Described floating block, material are the middle and higher density foamed plastics; The center is provided with the through hole that diameter is 1-4cm; Floating block can be purchased 10-20, and each can vertically be laid with 4 or 16 hollow manholes respectively on the floating block of central through hole periphery, and the manhole diameter is 1-3cm; Manhole inside is provided with the metal filter at 3-5cm place, distance floating block bottom, and the metal filter is a stainless steel cloth, width of mesh 2-5mm; The frozen pipe of seminal fluid is placed in metal filter top in the manhole.
Described handle, material are stainless steel, and the top is a square plate, and the center is provided with centre bore, are positioned on the floating block by the supporting leg support fixation, and square plate passes cover plate, are positioned at the housing upper port.
Described cover plate, material are stainless steel, and thickness is 0.3-1cm, and the center is provided with the square mesopore that handle is passed through, and cover plate is to lean on the support of the frozen pipe of each seminal fluid to be positioned at the floating block top.
Described spiral shell axle, the first half are the screw rod that is provided with screw thread, external diameter 6-20mm, pitch 0.5-3mm.
The present invention compares prior art and is characterized in:
1, reasonable idea of the present invention, delicate structure, easy and simple to handle, of many uses;
2, it is freezing to be suitable for open-air fish sperm sampling two-step method, the freezing preservation research of the programmed cooling of also can experimentize chamber fish sperm or other materials;
3, the present invention has brought very big facility for the sperm freezing preservation work at scientific research personnel and breeding production family, has overcome effectively to adopt that liquid nitrogen liquid level range estimation frozen error is big, the shortcoming of poor repeatability;
4, than other programmed cooling instrument, this device can carry separately after pouring liquid nitrogen into and use, and is easy to carry and operates, and is particularly suitable for field acquisition sample and emergent the use.
Description of drawings:
Fig. 1: structural representation of the present invention.
1-spiral shell axle, 2-housing, the 3-liquid nitrogen is minimum and high liquid level (HLL) line, 4-floating block, 5-central through hole, 6-filter, 7-handle, 8-cover plate, 9-spiral shell piece, 10-lock screw.
Embodiment:
Be described in detail structure of the present invention and using method in conjunction with the accompanying drawings below by embodiment:
1, structure of the present invention:
Shown in the accompanying drawing 1: its structure is to be made of spiral shell axle 1, housing 2, floating block 4, handle 7, cover plate 8, spiral shell piece 9; Housing 2 is that cylinder is cup-shaped, and material is a stainless steel, and height is 40cm, diameter is 35cm, and the upper end is uncovered, the lower end back cover, inwall below indicates the minimum of the liquid nitrogen of packing into and high liquid level (HLL) line 3, and minimum level line 3 is 7cm apart from the housing bottom surface, and high liquid level (HLL) line 3 is 12cm apart from the housing bottom surface; The middle part is provided with cylinder floating block 4 in the housing 2, and floating block 4 thickness are 8cm, and diameter is 32cm, and material is the middle and higher density foamed plastics, and the center is provided with the central through hole that diameter is 2.5cm; Vertically be laid with the hollow manhole 5 that 8 diameters are 1.5cm at floating block 4 central through hole peripheries, manhole 5 inside are provided with metal filter 6 at 4cm place, distance floating block bottom, the frozen pipe 11 of seminal fluid is positioned at manhole 5 metal filters 6 tops, metal filter 6 is a stainless steel cloth, width of mesh 3mm; The top of floating block 4 is provided with the handle 7 that is supported by supporting leg, the equipped cover plate 8 that makes spermatozoa cryopreservation pipe fixed-site in top; Handle 7 its effects, one can portablely propose floating block 4 or put into from housing 2, and two can move by the rotation of spiral shell piece 9, to align the position that floating block 4 and frozen pipe 11 integral body of seminal fluid move down; Handle 7 materials are stainless steel, are positioned on the floating block 4 by the supporting leg support fixation, and the top is a square plate, and the center is provided with centre bore, pass cover plate 8 and are positioned at housing 2 upper port; Cover plate 8 materials are stainless steel, and thickness is 0.5cm, are provided with the square mesopore that handle 7 is passed through, and cover plate 8 is to lean on the support of the frozen pipe 11 of each seminal fluid to be positioned at floating block 4 tops, and the spermatozoa cryopreservation pipe 11 of placement is fixed; Spiral shell axle 1 is by the vertical circular cylindrical shell 2 that is positioned at of centre bore of handle 7, cover plate 8 and floating block 4; Spiral shell axle 1 upper end is the screw rod that is provided with screw thread, external diameter 15mm, and pitch 2mm, the bottom of housing 2 is fixed in the lower end; Spiral shell piece 9 is the long column body, is fitted on the spiral shell axle 1 upper end screw rod, each side is provided with a lock screw 10,
2, using method of the present invention:
Before using this device frozen sperm, earlier liquid nitrogen is poured in this device, make liquid level between minimum and ceiling for accumulation metal line 3, hand-held then handle 7, the floating block 4 of placing the frozen pipe 11 of fish sperm will be arranged, penetrate the centre bore of floating block 4 and handle 7 by vertical spiral shell axle 1, put into housing 2 middle parts, and it is floated on the liquid nitrogen liquid level, bonnet upper cover plate 8 makes spermatozoa cryopreservation pipe 11 fixed-site, spiral shell piece 9 is installed on the spiral shell axle 1 again, rotation by spiral shell piece 9 is moved, and slowly moves down the frozen pipe 11 whole pan position down of levelling floating block 4 and seminal fluid; Because foamed plastics floating block 4 has fabulous buoyancy, therefore can make the frozen pipe 11 of seminal fluid on the floating block 4 interior metal filters 6 be positioned at 3-5cm on the liquid nitrogen liquid level all the time.Owing to the good thermal insulation property of floating block 4 foamed plastics, can completely cut off the rapid osmotic of liquid nitrogen cold air, can make the temperature in the circular circular hole 5 be unlikely to reduce rapidly.Be provided with lock screw 10 in spiral shell piece 9 left and right sides, can lock the position of floating block 4, the frozen pipe 11 of its seminal fluid is positioned on the liquid nitrogen liquid level, thereby finish the freezing preservation of program of seminal fluid.
Claims (6)
1. portable fish sperm frozen cooling device, the structure that it is characterized in that it is to be made of spiral shell axle 1, housing 2, floating block 4, handle 7, cover plate 8, spiral shell piece 9; Housing 2 is that cylinder is cup-shaped, and height is 30-60cm, and diameter is 25-50cm, and inwall below indicates the minimum of the liquid nitrogen of packing into and high liquid level (HLL) line 3; The middle and lower part is provided with cylinder floating block 4 in the housing 2, and thickness is 7-10cm, diameter 22-48cm; The top of floating block 4 is provided with the handle 7 that is supported by supporting leg, the equipped cover plate 8 that makes spermatozoa cryopreservation pipe 11 fixed-site in top; Spiral shell axle 1 is by the vertical circular cylindrical shell 2 that is positioned at of centre bore of handle 7, cover plate 8 and floating block 4, and the bottom of housing 2 is fixed in the lower end; Spiral shell piece 9 is the long column body, is fitted on the spiral shell axle 1 upper end screw rod, each side is provided with a lock screw 10.
2. a kind of portable fish sperm frozen cooling device according to claim 1 is characterized in that the minimum and high liquid level (HLL) line 3 of liquid nitrogen that described container inner wall below is provided with.Minimum level line-spacing housing 2 bottom surfaces are 7cm, and high liquid level (HLL) line-spacing housing 2 bottom surfaces are 12cm.
3. a kind of portable fish sperm frozen cooling device according to claim 1 is characterized in that described floating block 4, and material is the middle and higher density foamed plastics, and the center is provided with the through hole that diameter is 1-4cm; Floating block 4 can be purchased 10-20, and each can vertically be laid with the hollow manhole 5 that 4 or 16 diameters are 1-3cm respectively on the floating block 4 of central through hole periphery, and manhole 5 inside are provided with metal filter 6 at 3-5cm place, distance floating block 4 bottom; Metal filter 6 is a stainless steel cloth, width of mesh 2-5mm; The frozen pipe 11 of seminal fluid is placed in metal filter 6 tops in the manhole 5.
4. a kind of portable fish sperm frozen cooling device according to claim 1, it is characterized in that described handle 7, material is a stainless steel, the top is a square plate, the center is provided with centre bore, be positioned on the floating block 4 by the supporting leg support fixation, square plate passes cover plate 8, is positioned at housing 2 upper port.
5. a kind of portable fish sperm frozen cooling device according to claim 1, it is characterized in that described cover plate 8, material is a stainless steel, and thickness is 0.3-1cm, the center is provided with the square mesopore that handle 7 is passed through, and cover plate 8 is to lean on the support of the frozen pipe 11 of each seminal fluid to be positioned at floating block 4 tops.
6. a kind of portable fish sperm frozen cooling device according to claim 1 is characterized in that described spiral shell axle 1, and its first half is the screw rod that is provided with screw thread, external diameter 6-20mm, pitch 0.5-3mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101897389A CN101849535B (en) | 2010-05-24 | 2010-05-24 | Portable fish sperm frozen cooling device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101897389A CN101849535B (en) | 2010-05-24 | 2010-05-24 | Portable fish sperm frozen cooling device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101849535A true CN101849535A (en) | 2010-10-06 |
| CN101849535B CN101849535B (en) | 2013-04-17 |
Family
ID=42801377
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010101897389A Expired - Fee Related CN101849535B (en) | 2010-05-24 | 2010-05-24 | Portable fish sperm frozen cooling device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101849535B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103202286A (en) * | 2013-04-11 | 2013-07-17 | 天津开发区合普工贸有限公司 | Electronic program-controlled const-speed cooling and freezing device |
| CN106742745A (en) * | 2016-12-06 | 2017-05-31 | 山东大学 | A kind of device for being capable of achieving multiple batches of freezing of semen |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02200601A (en) * | 1989-01-30 | 1990-08-08 | Nozawagumi:Kk | Freezer of zygote, sperm or the like |
| CN101088511A (en) * | 2007-02-15 | 2007-12-19 | 中国水产科学研究院长江水产研究所 | Simple method for ultralow temperature preservation of fish sperm |
| CN101124444A (en) * | 2004-01-08 | 2008-02-13 | B·西克斯特 | Transport container for keeping frozen material chilled |
| CN101502256A (en) * | 2009-03-11 | 2009-08-12 | 宁波大学 | Ultra low temperature cryopreservation method for sperm of large yellow crocker and cryopreservation device |
| CN201365480Y (en) * | 2009-01-14 | 2009-12-23 | 上海交通大学医学院附属仁济医院 | Two-step ultra-low temperature hand-operated sperm programmable freezer |
-
2010
- 2010-05-24 CN CN2010101897389A patent/CN101849535B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02200601A (en) * | 1989-01-30 | 1990-08-08 | Nozawagumi:Kk | Freezer of zygote, sperm or the like |
| CN101124444A (en) * | 2004-01-08 | 2008-02-13 | B·西克斯特 | Transport container for keeping frozen material chilled |
| CN101088511A (en) * | 2007-02-15 | 2007-12-19 | 中国水产科学研究院长江水产研究所 | Simple method for ultralow temperature preservation of fish sperm |
| CN201365480Y (en) * | 2009-01-14 | 2009-12-23 | 上海交通大学医学院附属仁济医院 | Two-step ultra-low temperature hand-operated sperm programmable freezer |
| CN101502256A (en) * | 2009-03-11 | 2009-08-12 | 宁波大学 | Ultra low temperature cryopreservation method for sperm of large yellow crocker and cryopreservation device |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103202286A (en) * | 2013-04-11 | 2013-07-17 | 天津开发区合普工贸有限公司 | Electronic program-controlled const-speed cooling and freezing device |
| CN103202286B (en) * | 2013-04-11 | 2015-09-02 | 天津开发区合普工贸有限公司 | Electronic program controls at the uniform velocity to lower the temperature freezing plant |
| CN106742745A (en) * | 2016-12-06 | 2017-05-31 | 山东大学 | A kind of device for being capable of achieving multiple batches of freezing of semen |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101849535B (en) | 2013-04-17 |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20171214 Address after: 536000 the Guangxi Zhuang Autonomous Region Beihai Seaview Avenue (Zhu Linduan) marine scientific research innovation park, one road B1-1 building Patentee after: Beihai Nanfang marine science and Technology Development Co.,Ltd. Address before: 266071 Shandong Province, Qingdao city Nanjing Road No. 106 Patentee before: YELLOW SEA FISHERIES Research Institute CHINESE ACADEMY OF FISHERY SCIENCES |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130417 |