CN101849535B - Portable fish sperm frozen cooling device - Google Patents
Portable fish sperm frozen cooling device Download PDFInfo
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- CN101849535B CN101849535B CN2010101897389A CN201010189738A CN101849535B CN 101849535 B CN101849535 B CN 101849535B CN 2010101897389 A CN2010101897389 A CN 2010101897389A CN 201010189738 A CN201010189738 A CN 201010189738A CN 101849535 B CN101849535 B CN 101849535B
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- floating block
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- housing
- spiral shell
- cooling device
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Abstract
The invention relates to a portable fish sperm frozen cooling device which comprises a screw axis, a casing, a floating block, a hanging ring, a cover plate and a screw block. The casing is in the shape of a cylindrical cup, a lowest liquid level line and a highest liquid level line of liquid nitrogen loading are marked at the lower part of the inner wall. The middle lower part in the casing is provided with the cylinder floating block; the top part of the floating block is provided with the hanging ring supported by landing legs, and the upper part is provided with the cover plate which can fix the position of a sperm freezing tube; the screw axis passes through the central holes of the hanging ring, the cover plate and the floating block and is vertically positioned in the casing, and the lower end of the screw axis is fixed at the bottom of the casing; and the screw block is assembled on a screw rod of the upper end of the screw axis, and both sides of the crew block are respectively provided with a locking bolt. The invention has reasonable concept, elaborate structure, convenient operation and convenient carrying and use, can effectively overcome the defects of big frozen error and bad repeatability by eyeballing according to liquid nitrogen liquid level, is suitable for sperm sampling two-step freezing of wild fish, and is also suitable for researchers to carry out laboratory precision research of cooling cryopreservation on fish sperm and other materials.
Description
Technical field:
The invention belongs to fish germ plasma resource ultralow temperature and preserve equipment, is a kind of portable fish sperm freezing programmed cooling instrument.
The fish sperm Long-term Cryopreservation is preserved and the aquaculture of improved seeds has far-reaching theory significance and using value fish germ plasma resource.To the freezing preservation of some Important Economic fish and endangered fish sperm, mainly adopt easy two-step method, multistep processes and programmed cooling method at present.The above two are finished to the distance of liquid nitrogen liquid level by artificial manually control seminal fluid cryopreservation tube usually, will rely on to a great extent operator's experience in operating process, therefore often exist observe difficult, error is large, the repeated shortcoming such as low of refrigerating effect; Latter needs complexity, expensive programmed cooling instrument to realize, such as the silent program control cooling instrument of CryoMed Freezers that flies your company's production of generation of U.S.'s match, its principle is to utilize pump and the flow velocity of solenoid control liquid nitrogen in cooling recirculation system to reach the purpose of programmed cooling, because it has passed through the reliable control of computer programming controller, thereby can realize accurately freezing from the cell to embryo; But its shortcoming be complex structure, bulky, equipment is heavy, price is high, and be subject to the constraint such as instruments weight, size and be difficult to field sampling research.
Summary of the invention
The purpose of this invention is to provide a kind of portable fish sperm freezing programmed cooling instrument, to satisfy the needs of the accurate research in field sampling and laboratory.
Its structure is to be made of spiral shell axle, housing, floating block, handle, cover plate, spiral shell piece; Housing is that cylinder is cup-shaped, and height is 30-60cm, and diameter is 25-50cm, and inwall below indicates the minimum of the liquid nitrogen of packing into and high liquid level (HLL) line; The middle and lower part is provided with the cylinder floating block in the housing, and thickness is 7-10cm; Diameter 22-48cm; The top of floating block is provided with the handle that is supported by supporting leg, the equipped fixing cover plate in spermatozoa cryopreservation pipe position that makes in top; The spiral shell axle is by the vertical housing that is positioned at of centre bore of handle, cover plate and floating block, and the bottom of housing is fixed in the lower end; The spiral shell piece is the long column body, is fitted on the screw rod of spiral shell axle upper end, each side is provided with a lock screw.
Minimum and the high liquid level (HLL) line of the liquid nitrogen that described container inner wall below arranges, minimum level line-spacing housing bottom surface is 7cm, high liquid level (HLL) line-spacing housing bottom surface is 12cm.
Described floating block, material are the middle and higher density foamed plastics; The center is provided with the through hole that diameter is 1-4cm; Floating block can be purchased 10-20, and each can vertically be laid with respectively 4 or 16 hollow manholes on the floating block of central through hole periphery, and the manhole diameter is 1-3cm; Manhole inside is provided with the metal filter at 3-5cm place, distance floating block bottom, and the metal filter is stainless steel cloth, width of mesh 2-5mm; The seminal fluid cryopreservation tube is placed in metal filter top in the manhole.
Described handle, material are stainless steel, and the top is a square plate, and the center is provided with centre bore, are supported being fixed on the floating block by supporting leg, and square plate passes cover plate, is positioned at the housing upper port.
Described cover plate, material are stainless steel, and thickness is 0.3-1cm, and the center is provided with the square mesopore that handle is passed through, and cover plate is to lean on the support of each seminal fluid cryopreservation tube to be positioned at the floating block top.
Described spiral shell axle, the first half is for establishing threaded screw rod, external diameter 6-20mm, pitch 0.5-3mm.
The present invention compares prior art and is characterized in:
1, reasonable idea of the present invention, delicate structure, easy and simple to handle, of many uses;
2, it is freezing to be suitable for open-air fish sperm sampling two-step method, also can carry out the freezing preservation research of programmed cooling of laboratory fish sperm or other materials;
3, the present invention brings great convenience to the sperm freezing preservation work at scientific research personnel and breeding production family, has effectively overcome to adopt that liquid nitrogen liquid level range estimation frozen error is large, the shortcoming of poor repeatability;
4, than other programmed cooling instrument, this device can carry separately after pouring liquid nitrogen into and use, and is easy to carry and operates, and is particularly suitable for field acquisition sample and Emergency use.
Description of drawings:
Fig. 1: structural representation of the present invention.
1-spiral shell axle, 2-housing, the 3-liquid nitrogen is minimum and high liquid level (HLL) line, 4-floating block, 5-central through hole, 6-filter, 7-handle, 8-cover plate, 9-spiral shell piece, 10-lock screw.
Embodiment:
Be described in detail by reference to the accompanying drawings structure of the present invention and using method below by embodiment:
1, structure of the present invention:
Shown in the accompanying drawing 1: its structure is to be made of spiral shell axle 1, housing 2, floating block 4, handle 7, cover plate 8, spiral shell piece 9; Housing 2 is that cylinder is cup-shaped, and material is stainless steel, and height is 40cm, diameter is 35cm, and the upper end is uncovered, the lower end back cover, inwall below indicates the minimum of the liquid nitrogen of packing into and high liquid level (HLL) line 3, and minimum level line 3 is 7cm apart from the housing bottom surface, and high liquid level (HLL) line 3 is 12cm apart from the housing bottom surface; Housing 2 interior middle parts are provided with cylinder floating block 4, and floating block 4 thickness are 8cm, and diameter is 32cm, and material is the middle and higher density foamed plastics, and the center is provided with the central through hole that diameter is 2.5cm; Vertically be laid with the hollow manhole 5 that 8 diameters are 1.5cm at floating block 4 central through hole peripheries, manhole 5 inside are provided with metal filter 6 at 4cm place, distance floating block bottom, seminal fluid cryopreservation tube 11 is positioned at manhole 5 metal filters 6 tops, metal filter 6 is stainless steel cloth, width of mesh 3mm; The top of floating block 4 is provided with the handle 7 that is supported by supporting leg, the equipped fixing cover plate 8 in spermatozoa cryopreservation pipe position that makes in top; Handle 7 its effects, one can portablely propose floating block 4 or put into from housing 2, and two can be by the rotary moving of spiral shell piece 9, the position that moves down to align floating block 4 and seminal fluid cryopreservation tube 11 integral body; Handle 7 materials are stainless steel, are supported being fixed on the floating block 4 by supporting leg, and the top is a square plate, and the center is provided with centre bore, passes cover plate 8 and is positioned at housing 2 upper port; Cover plate 8 materials are stainless steel, and thickness is 0.5cm, are provided with the square mesopore that handle 7 is passed through, and cover plate 8 is to lean on the support of each seminal fluid cryopreservation tube 11 to be positioned at floating block 4 tops, and the spermatozoa cryopreservation pipe 11 of placement is fixed; Spiral shell axle 1 is by the vertical circular cylindrical shell 2 that is positioned at of centre bore of handle 7, cover plate 8 and floating block 4; Spiral shell axle 1 upper end is for establishing threaded screw rod, external diameter 15mm, and pitch 2mm, the bottom of housing 2 is fixed in the lower end; Spiral shell piece 9 is the long column body, is fitted on the spiral shell axle 1 upper end screw rod, each side is provided with a lock screw 10,
2, using method of the present invention:
Before using this device frozen sperm, liquid nitrogen is poured in this device first, make liquid level between minimum and ceiling for accumulation metal line 3, then hand handle 7, the floating block 4 of placing fish sperm cryopreservation tube 11 will be arranged, penetrated the centre bore of floating block 4 and handle 7 by vertical spiral shell axle 1, put into housing 2 middle parts, and it is floated on the liquid nitrogen liquid level, bonnet upper cover plate 8 is fixed spermatozoa cryopreservation pipe 11 positions, spiral shell piece 9 is installed on the spiral shell axle 1 again, by the rotary moving of spiral shell piece 9, slowly move down levelling floating block 4 and seminal fluid cryopreservation tube 11 whole lower pan position; Because foamed plastics floating block 4 has fabulous buoyancy, therefore can make the seminal fluid cryopreservation tube 11 on the floating block 4 interior metal filters 6 be positioned at all the time 3-5cm on the liquid nitrogen liquid level.Owing to the good thermal insulation property of floating block 4 foamed plastics, can completely cut off the rapid osmotic of liquid nitrogen cold air, can make the temperature in the circular circular hole 5 be unlikely to reduce rapidly.Be provided with lock screw 10 in spiral shell piece 9 left and right sides, can lock the position of floating block 4, its seminal fluid cryopreservation tube 11 is positioned on the liquid nitrogen liquid level, thereby finish the freezing preservation of program of seminal fluid.
Claims (5)
1. portable fish sperm frozen cooling device, the structure that it is characterized in that it are to be made of spiral shell axle (1), housing (2), floating block (4), handle (7), cover plate (8), spiral shell piece (9); Housing (2) is that cylinder is cup-shaped, and height is 30-60cm, and diameter is 25-50cm, and inwall below indicates the minimum of the liquid nitrogen of packing into and high liquid level (HLL) line (3); The interior middle and lower part of housing (2) is provided with cylinder floating block (4), and thickness is 7-10cm, diameter 22-48cm; The top of floating block (4) is provided with the handle (7) that is supported by supporting leg, the equipped fixing cover plate (8) in spermatozoa cryopreservation pipe (11) position that makes in top; Spiral shell axle (1) is by the vertical circular cylindrical shell (2) that is positioned at of centre bore of handle (7), cover plate (8) and floating block (4), and the bottom of housing (2) is fixed in the lower end; Spiral shell piece (9) is the long column body, is fitted on the screw rod of spiral shell axle (1) upper end, each side is provided with a lock screw (10),
Described floating block (4), material are the middle and higher density foamed plastics; The center is provided with the through hole that diameter is 1-4cm; Floating block (4) can be purchased 10-20, each can vertically be laid with respectively the hollow manhole (5) that 4 or 16 diameters are 1-3cm on the floating block (4) of central through hole periphery, manhole (5) is inner to be provided with metal filter (6) at 3-5cm place, distance floating block (4) bottom; Metal filter (6) is stainless steel cloth, width of mesh 2-5mm; Seminal fluid cryopreservation tube (11) is placed in the interior metal filter of manhole (5) (6) top.
2. a kind of portable fish sperm frozen cooling device according to claim 1, it is characterized in that the minimum and high liquid level (HLL) index line (3) of liquid nitrogen that described container inner wall below arranges, minimum level line-spacing housing (2) bottom surface is 7cm, and high liquid level (HLL) line-spacing housing (2) bottom surface is 12cm.
3. a kind of portable fish sperm frozen cooling device according to claim 1, it is characterized in that described handle (7), material is stainless steel, the top is a square plate, the center is provided with centre bore, be fixed on the floating block (4) by the supporting leg support, square plate passes cover plate (8), is positioned at housing (2) upper port.
4. a kind of portable fish sperm frozen cooling device according to claim 1, it is characterized in that described cover plate (8), material is stainless steel, thickness is 0.3-1cm, the center is provided with the square mesopore that handle (7) is passed through, and cover plate (8) is to lean on the support of each seminal fluid cryopreservation tube (11) to be positioned at floating block (4) top.
5. a kind of portable fish sperm frozen cooling device according to claim 1 is characterized in that described spiral shell axle (1), and its first half is for establishing threaded screw rod, external diameter 6-20mm, pitch 0.5-3mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101897389A CN101849535B (en) | 2010-05-24 | 2010-05-24 | Portable fish sperm frozen cooling device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101897389A CN101849535B (en) | 2010-05-24 | 2010-05-24 | Portable fish sperm frozen cooling device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101849535A CN101849535A (en) | 2010-10-06 |
| CN101849535B true CN101849535B (en) | 2013-04-17 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010101897389A Active CN101849535B (en) | 2010-05-24 | 2010-05-24 | Portable fish sperm frozen cooling device |
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| CN (1) | CN101849535B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103202286B (en) * | 2013-04-11 | 2015-09-02 | 天津开发区合普工贸有限公司 | Electronic program controls at the uniform velocity to lower the temperature freezing plant |
| CN106742745B (en) * | 2016-12-06 | 2018-03-23 | 山东大学 | A kind of device that multiple batches of freezing of semen can be achieved |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101088511A (en) * | 2007-02-15 | 2007-12-19 | 中国水产科学研究院长江水产研究所 | Simple method for ultralow temperature preservation of fish sperm |
| CN101124444A (en) * | 2004-01-08 | 2008-02-13 | B·西克斯特 | Transport container for keeping frozen material chilled |
| CN101502256A (en) * | 2009-03-11 | 2009-08-12 | 宁波大学 | Ultra low temperature cryopreservation method for sperm of large yellow crocker and cryopreservation device |
| CN201365480Y (en) * | 2009-01-14 | 2009-12-23 | 上海交通大学医学院附属仁济医院 | Two-step ultra-low temperature hand-operated sperm programmable freezer |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02200601A (en) * | 1989-01-30 | 1990-08-08 | Nozawagumi:Kk | Freezer of zygote, sperm or the like |
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2010
- 2010-05-24 CN CN2010101897389A patent/CN101849535B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101124444A (en) * | 2004-01-08 | 2008-02-13 | B·西克斯特 | Transport container for keeping frozen material chilled |
| CN101088511A (en) * | 2007-02-15 | 2007-12-19 | 中国水产科学研究院长江水产研究所 | Simple method for ultralow temperature preservation of fish sperm |
| CN201365480Y (en) * | 2009-01-14 | 2009-12-23 | 上海交通大学医学院附属仁济医院 | Two-step ultra-low temperature hand-operated sperm programmable freezer |
| CN101502256A (en) * | 2009-03-11 | 2009-08-12 | 宁波大学 | Ultra low temperature cryopreservation method for sperm of large yellow crocker and cryopreservation device |
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| CN101849535A (en) | 2010-10-06 |
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Effective date of registration: 20171214 Address after: 536000 the Guangxi Zhuang Autonomous Region Beihai Seaview Avenue (Zhu Linduan) marine scientific research innovation park, one road B1-1 building Patentee after: Beihai Nanfang marine science and Technology Development Co Ltd Address before: 266071 Shandong Province, Qingdao city Nanjing Road No. 106 Patentee before: Inst of Huanghai Sea Marine Products, Chinese Academy of Aquatic Product Science |