CN110192551A - Cooling system and control method used in a kind of stem cell cryopreserving process - Google Patents
Cooling system and control method used in a kind of stem cell cryopreserving process Download PDFInfo
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- CN110192551A CN110192551A CN201811620380.3A CN201811620380A CN110192551A CN 110192551 A CN110192551 A CN 110192551A CN 201811620380 A CN201811620380 A CN 201811620380A CN 110192551 A CN110192551 A CN 110192551A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0278—Physical preservation processes
- A01N1/0284—Temperature processes, i.e. using a designated change in temperature over time
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- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
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- Devices That Are Associated With Refrigeration Equipment (AREA)
Abstract
The invention belongs to freeze control system technical field, disclose cooling system and control method used in a kind of stem cell cryopreserving process, including temperature control modules, rate of temperature fall control module, refrigeration module, temperature monitoring module, leakproofness monitoring modular, rate of temperature fall monitoring modular, display module；The temperature value and rate of temperature fall of decline needed for setting stem cell cryopreserving by temperature control modules and rate of temperature fall control module；The cooling of stem cell cryopreserving environment is carried out according to the temperature value and rate of temperature fall of setting by refrigeration module；The parameter in the temperature-fall period of stem cell cryopreserving environment is monitored by temperature monitoring module, leakproofness monitoring modular and rate of temperature fall monitoring modular, obtains the data of temperature value, rate of temperature fall, leakage rate；It will acquire data by display module to show, be supplied to staff's analysis and regulation；The data that will acquire are transferred to through temperature control modules and rate of temperature fall control module, carry out system intelligent control.
Description
Technical field
Cool down used in control system technical field more particularly to a kind of stem cell cryopreserving process the invention belongs to freeze
System and control method.
Background technique
Currently, the purpose that the prior art commonly used in the trade is such that freezen protective is subtracted under the low temperature environment of liquid nitrogen
The metabolic activity of slow cell simultaneously sustains life；In the refrigerating process of cell suspension, if frozen, rate is too fast cannot to be allowed carefully
Moisture intracellular goes out born of the same parents completely, and so as to cause intracellular ice crystal is formed and causes to damage to cell, the formation of ice crystal is to cause
One of the main reason for cell death；Frozen stock solution has certain toxic effect to cell, freezes the too low cell that will lead to of rate and is freezing
In liquid storage exposure overlong time and cause cell by poison；Traditional compression refigerating machine components are more, and noise is big, uses refrigerant
Not only cause environmental pollution and also for temperature control it is inflexible, be not suitable for stem cell cryopreserving；
In conclusion problem of the existing technology is:
(1) freezing rate is not controlled in traditional refrigeration system, in the refrigerating process of cell suspension, if
It is too fast and intracellular ice crystal is caused to be formed and cause to damage to cell to freeze rate；The too low cell that will lead to of rate is frozen to freeze
In liquid storage exposure overlong time and cause cell by poison.
(2) traditional refrigerator temperature rate control system has non-linear, time variation, it is difficult to realize that high-precision is controlled
System, is easy to form ice crystal during stem cell cryopreserving, damage to the stem cell for depositing jelly.
(3) it not to the detection of leakproofness in traditional refrigeration system, is not only easy to generate extra heat with external environment
Exchange influences rate of temperature fall and can seriously affect cell safety to microbial contamination is generated.
Summary of the invention
In view of the problems of the existing technology, the present invention provides cooling systems used in a kind of stem cell cryopreserving process
And control method.
The invention is realized in this way cooling system used in stem cell cryopreserving process includes:
Temperature control modules, rate of temperature fall control module, refrigeration module, temperature monitoring module, leakproofness monitoring modular, drop
Warm rate monitoring modular, display module.
Temperature control modules are connect with refrigeration module；Temperature control modules can control the temperature frozen, carry out setting and
Refrigeration machine is opened, refrigeration machine used in refrigeration module is semiconductor cooler.
Rate of temperature fall control module is connect with refrigeration module, and rate of temperature fall control module can control at a temperature of refrigeration module
The rate of drop guarantees that cell is injury-free.
Refrigeration module is connect with temperature monitoring module, and refrigeration module is connect with leakproofness monitoring modular, refrigeration module and drop
Warm rate monitoring modular connection；Temperature monitoring module can monitor the real time temperature in temperature-fall period, and leakproofness monitoring modular can be real
When monitoring leakproofness guarantee to cool down in strict accordance with the rate of setting, rate of temperature fall monitoring modular can reduction of speed under continuous monitoring temperature
Rate.
Temperature monitoring module is connect with display module, and leakproofness monitoring modular is connect with display module, rate of temperature fall monitoring
Module is connect with display module, and display module can show monitoring data, facilitates artificial regulatory system.
Temperature monitoring module is connect with temperature control modules, and temperature monitoring module passes real time data back temperature control mould
Block carries out temperature regulation.
Rate of temperature fall monitoring modular is connect with rate of temperature fall control module；Rate of temperature fall monitoring modular passes real time data back
Rate of temperature fall control module guarantees the steady of rate of temperature fall.
The control method of cooling system used in stem cell cryopreserving process the following steps are included:
Step S101: decline needed for setting stem cell cryopreserving by temperature control modules and rate of temperature fall control module
Temperature value and rate of temperature fall.
Step S102: the drop of stem cell cryopreserving environment is carried out according to the temperature value and rate of temperature fall of setting by refrigeration module
Temperature.
Step S103: stem cell is frozen by temperature monitoring module, leakproofness monitoring modular and rate of temperature fall monitoring modular
Parameter in the temperature-fall period of dis environment is monitored, and obtains the data of temperature value, rate of temperature fall, leakage rate.
Step S104: the temperature value that will acquire by display module, rate of temperature fall, leakage rate data show, mention
Supply staff's analysis and regulation.
Step S105: the temperature that will acquire by temperature monitoring module, leakproofness monitoring modular and rate of temperature fall monitoring modular
Angle value, rate of temperature fall, leakage rate data be transferred to through temperature control modules and rate of temperature fall control module, carry out system intelligence
It can regulation.
The positive effect of the present invention has:
(1) control by setting to freezing rate can flexibly control temperature, control rate of temperature fall, and real-time monitoring,
Avoid stem cell because cooling rate is too fast and it is excessively slow due to caused by destruction, improve the quality of frozen cell.
(2) by using PID control to refrigerator temperature rate control system, local pole is easily fallen into for single neuron
The defect of small value improves the initial value of weighting coefficient, effectively increases the control precision of temperature rate, avoids and is depositing jelly
It is injured caused by stem cell in the process.
(3) present invention is obtained by setting sealing propertytest module using the algorithm for calculating pressure monitoring sealing performance
Leakproofness result is sensitive effectively to ensure that the sealing performance of refrigeration module, avoid refrigeration space and external environment generate it is more
Waste heat exchange, it is more efficient to the control of rate of temperature fall, pollution of the microorganism to cell is avoided, ensure that the safety of cell.
Detailed description of the invention
Fig. 1 is that cooling system used in stem cell cryopreserving process provided in an embodiment of the present invention and control method structure are shown
It is intended to.
Fig. 2 is cooling system schematic diagram used in stem cell cryopreserving process provided in an embodiment of the present invention.
In figure: 1, temperature control modules；2, rate of temperature fall control module；3, refrigeration module；4, temperature monitoring module；5, close
Envelope property monitoring modular；6, rate of temperature fall monitoring modular；7, display module.
Specific embodiment
In order to further understand the content, features and effects of the present invention, the following examples are hereby given, and cooperate attached drawing
Detailed description are as follows.
Structure of the invention is explained in detail with reference to the accompanying drawing.
As shown in Figure 1, the control method of cooling system used in stem cell cryopreserving process provided in an embodiment of the present invention
The following steps are included:
S101: the temperature of decline needed for setting stem cell cryopreserving by temperature control modules and rate of temperature fall control module
Value and rate of temperature fall.
S102: the cooling of stem cell cryopreserving environment is carried out according to the temperature value and rate of temperature fall of setting by refrigeration module.
S103: by temperature monitoring module, leakproofness monitoring modular and rate of temperature fall monitoring modular to stem cell cryopreserving ring
Parameter in the temperature-fall period in border is monitored, and obtains the data of temperature value, rate of temperature fall, leakage rate.
S104: the temperature value that will acquire by display module, rate of temperature fall, leakage rate data show, be supplied to
Staff's analysis and regulation.
S105: the temperature value that will acquire by temperature monitoring module, leakproofness monitoring modular and rate of temperature fall monitoring modular,
Rate of temperature fall, leakage rate data be transferred to through temperature control modules and rate of temperature fall control module, carry out system and intelligently adjust
Control.
As shown in Fig. 2, cooling system used in stem cell cryopreserving process provided in an embodiment of the present invention includes: temperature control
Molding block 1, rate of temperature fall control module 2, refrigeration module 3, temperature monitoring module 4, leakproofness monitoring modular 5, rate of temperature fall prison
Survey module 6, display module 7.
Temperature control modules 1 are connect with refrigeration module 3；Temperature control modules can control the temperature frozen, be set
With open refrigeration machine, refrigeration machine used in refrigeration module is semiconductor cooler.
Rate of temperature fall control module 2 is connect with refrigeration module 3, and rate of temperature fall control module can control refrigeration module temperature
The rate of decline guarantees that cell is injury-free.
Refrigeration module 3 is connect with temperature monitoring module 4, and refrigeration module 3 is connect with leakproofness monitoring modular 5, refrigeration module 3
It is connect with rate of temperature fall monitoring modular 6；Temperature monitoring module can monitor the real time temperature in temperature-fall period, leakproofness monitoring modular
Energy real-time monitoring leakproofness guarantees to cool down in strict accordance with the rate of setting, and rate of temperature fall monitoring modular can continuously monitoring temperature decline
Rate.
Temperature monitoring module 4 is connect with display module 7, and leakproofness monitoring modular 5 is connect with display module 7, rate of temperature fall
Monitoring modular 6 is connect with display module 7, and display module can show monitoring data, facilitates artificial regulatory system.
Temperature monitoring module 4 is connect with temperature control modules 1, and temperature monitoring module passes real time data back temperature control mould
Block carries out temperature regulation.
Rate of temperature fall monitoring modular 6 is connect with rate of temperature fall control module 2；Rate of temperature fall monitoring modular passes real time data
Rate of temperature fall control module is returned, guarantees the steady of rate of temperature fall.
The above is only the preferred embodiments of the present invention, and is not intended to limit the present invention in any form,
Any simple modification made to the above embodiment according to the technical essence of the invention, equivalent variations and modification, belong to
In the range of technical solution of the present invention.
Claims (2)
1. cooling system and control method used in a kind of stem cell cryopreserving process, which is characterized in that the stem cell is frozen
Depositing cooling system used in process includes:
Temperature control modules, rate of temperature fall control module, refrigeration module, temperature monitoring module, leakproofness monitoring modular, cooling speed
Rate monitoring modular, display module.
Temperature control modules are connect with refrigeration module；Temperature control modules can control the temperature frozen, set and opened
Refrigeration machine, refrigeration machine used in refrigeration module are semiconductor cooler.
Rate of temperature fall control module is connect with refrigeration module, and rate of temperature fall control module can control the decline of refrigeration module temperature
Rate guarantees that cell is injury-free.
Refrigeration module is connect with temperature monitoring module, and refrigeration module is connect with leakproofness monitoring modular, refrigeration module and cooling speed
The connection of rate monitoring modular；Temperature monitoring module can monitor the real time temperature in temperature-fall period, and leakproofness monitoring modular can supervise in real time
It surveys leakproofness to guarantee to cool down in strict accordance with the rate of setting, rate of temperature fall monitoring modular can continuous monitoring temperature fall off rate.
Temperature monitoring module is connect with display module, and leakproofness monitoring modular is connect with display module, rate of temperature fall monitoring modular
It is connect with display module, display module can show monitoring data, facilitate artificial regulatory system.
Temperature monitoring module is connect with temperature control modules, and temperature monitoring module passes real time data back temperature control modules, into
Trip temperature regulation.
Rate of temperature fall monitoring modular is connect with rate of temperature fall control module；Rate of temperature fall monitoring modular passes real time data back cooling
Rate control module guarantees the steady of rate of temperature fall.
2. cooling system and control method used in stem cell cryopreserving process as described in claim 1, which is characterized in that institute
State the control method of cooling system used in stem cell cryopreserving process the following steps are included:
Step 1: the temperature value of decline needed for setting stem cell cryopreserving by temperature control modules and rate of temperature fall control module and
Rate of temperature fall, temperature control modules use digital incremental algorithm to the control of temperature, specifically:
Δ u=u(k)-u(k-1)
=Kp[e(k)-e(k-1)]+Ki e(k)+Kd[e(k)-2e(k-1)+e(k-2)]
Wherein:eIt (k) is the difference of measured value and given value；KpFor proportionality coefficient；KiFor integral coefficient；KdFor differential coefficient；
The control method of rate of temperature fall control module is the input/output amount of temperature control system, for heating and cooling speed per minute
Rate uses Vset、VoutIt indicates,
In formula: usIt (k) is Seebeck reverse potential；R is three-level thermoelectric cooler all-in resistance；αiFor i-stage galvanic couple arm Seebeck system
Number；
Step 2: the cooling of stem cell cryopreserving environment is carried out according to the temperature value and rate of temperature fall of setting by refrigeration module；
Step 3: by temperature monitoring module, leakproofness monitoring modular and rate of temperature fall monitoring modular to stem cell cryopreserving environment
Temperature-fall period in parameter be monitored, obtain temperature value, rate of temperature fall, leakage rate data, leakproofness monitoring modular master
Leakage rate, pressure modification are calculated and the amendment to pressure are as follows:
In formula: T1、T0- it is thermodynamic temperature (T0=293.15K)；P0=400KPa；P1- in T1At a temperature of the pressure that needs to inflate
Force value；
Slip is calculated using static drop method after being modified to pressure, specifically:
In formula, Q-slip；Pt1- by t1The pressure assembly pressure gauge reading combination t that moment reads1The environment temperature at moment turns
It is changed to corresponding pressure value under the conditions of 293.15K；Pt0- by t0The pressure assembly pressure gauge reading combination t that moment reads0Moment
Environment temperature be converted to corresponding pressure value under the conditions of 293.15K；Ve- dischargeable capacity；
Step 4: the temperature value that will acquire by display module, rate of temperature fall, leakage rate data show, be supplied to work
Make personnel's analysis and regulation；
Step 5: the temperature value that will acquire by temperature monitoring module, leakproofness monitoring modular and rate of temperature fall monitoring modular, drop
Warm rate, the data of leakage rate are transferred to through temperature control modules and rate of temperature fall control module, carry out system intelligent control.
In formula, Q-slip；Pt1- by t1The pressure assembly pressure gauge reading combination t that moment reads1The environment temperature at moment；
Be converted to corresponding pressure value under the conditions of 293.15K；Pt0- by t0The pressure assembly pressure gauge reading combination t that moment reads0When
The environment temperature at quarter；Be converted to corresponding pressure value under the conditions of 293.15K；Ve- dischargeable capacity.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811620380.3A CN110192551A (en) | 2018-12-28 | 2018-12-28 | Cooling system and control method used in a kind of stem cell cryopreserving process |
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| CN201811620380.3A CN110192551A (en) | 2018-12-28 | 2018-12-28 | Cooling system and control method used in a kind of stem cell cryopreserving process |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110864497A (en) * | 2019-12-25 | 2020-03-06 | 重庆斯克特科技有限公司 | Cooling system and method for heating sealing ring in front box bearing hole of transmission |
| CN117603809A (en) * | 2023-12-06 | 2024-02-27 | 威海紫光科技园有限公司 | Temperature control method and system applied to resuscitation in NK cell preparation process |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102023070A (en) * | 2009-09-15 | 2011-04-20 | 珠海格力电器股份有限公司 | Device for mounting and checking pressure sensor and method for correcting pressure sensor |
| CN103202286A (en) * | 2013-04-11 | 2013-07-17 | 天津开发区合普工贸有限公司 | Electronic program-controlled const-speed cooling and freezing device |
| CN205124851U (en) * | 2015-11-24 | 2016-04-06 | 广州吉妮欧生物科技有限公司 | Cell freezing storage device |
| US20180055042A1 (en) * | 2015-09-24 | 2018-03-01 | Alberto Jose Sarmentero Ortiz | Portable, insulated capsules for cryopreservation of biological materials |
-
2018
- 2018-12-28 CN CN201811620380.3A patent/CN110192551A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102023070A (en) * | 2009-09-15 | 2011-04-20 | 珠海格力电器股份有限公司 | Device for mounting and checking pressure sensor and method for correcting pressure sensor |
| CN103202286A (en) * | 2013-04-11 | 2013-07-17 | 天津开发区合普工贸有限公司 | Electronic program-controlled const-speed cooling and freezing device |
| US20180055042A1 (en) * | 2015-09-24 | 2018-03-01 | Alberto Jose Sarmentero Ortiz | Portable, insulated capsules for cryopreservation of biological materials |
| CN205124851U (en) * | 2015-11-24 | 2016-04-06 | 广州吉妮欧生物科技有限公司 | Cell freezing storage device |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110864497A (en) * | 2019-12-25 | 2020-03-06 | 重庆斯克特科技有限公司 | Cooling system and method for heating sealing ring in front box bearing hole of transmission |
| CN110864497B (en) * | 2019-12-25 | 2024-01-30 | 重庆斯克特科技有限公司 | Transmission front box bearing hole heating seal ring cooling system and method |
| CN117603809A (en) * | 2023-12-06 | 2024-02-27 | 威海紫光科技园有限公司 | Temperature control method and system applied to resuscitation in NK cell preparation process |
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Application publication date: 20190903 |